| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 264,110 | G→A | 41.6% | Q287Q (CAG→CAA) | gabD‑I → | succinate‑semialdehyde dehydrogenase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 264,110 | 0 | G | A | 41.6% | 11.1 / 22.1 | 24 | Q287Q (CAG→CAA) | gabD‑I | succinate‑semialdehyde dehydrogenase |
| Reads supporting (aligned to +/- strand): ref base G (6/8); new base A (4/6); total (10/14) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
CGCCAAGGACATCAAGAAGGTTTCCCTGGAGCTGGGTGGCAACGCCCCGTTCATCGTGTTCGACGACGCCGACCTGGACAAGGCGGTCGAGGGCGCGATCATCTCCAAGTACCGTAACAACGGCCAGACCTGCGTCTGCGCCAACCGTATCTACGTGCAGGACGGCGTCTACGACGCGTTCGCCGAGAAGCTGGCCGCTGCAGTTGCCAAGCTGAAGATCGGTAACGGCCTGGAAGAAGGCACCACCACTGGCCCGCTG > NC_002947/263984‑264242 | cgcCAAGGACATCAAGAAGGTTTCCCTGGAGCTGGGTGGCAACGCCCCGTTCATCGTGTTCGACGACGCCGACCTGGACAAGGCGGTCGAGGGCGCGATCATCTCCAAGAACCGTAACAACGGCCAGACCTGCgaa > 1:510322/1‑134 (MQ=255) gcCAAGGACATCAAGAAGGTTTCCCTGGAGCTGGGTGGCAACGCCCCGTTCATCGTGTTCGACGACGCCGACCTGGACAAGGCGGTCGAGGGCGCGATCATCTCCAAGTACCGTAACAACGGCCAGACCTGCGTCt < 1:469427/136‑1 (MQ=255) gccgaccATCAAGAAGCTTGGGCTTGAGCTTGGCGGCAACGCGCCTTTCATCGTGTTCGACGACGCCGATCTCGATGCCGCCGTCGAAGGTGCTCTTATCGCCAAATTCCGCAACAACGGCCAAACCTGCGTCTgc < 2:124196/130‑1 (MQ=255) cAAGGACATCAAGAAGGTTTCCCTGGAGCTGGGTGGCAACGCCCCGTTCATCGTGTTCGACGACGCCGACCTGGACAAGGCGGTCGAGGGCGCGATCATCTCCAAGTACCGTAACAACGGCCAGACCTGCGTCTgc < 2:51321/136‑1 (MQ=255) cgaccATCAAGAAGCTTGGGCTTGAGCTTGGCGGCAACGCGCCTTTCATCGTGTTCGACGACGCCGATCTCGATGCCGCCGTCGAAGGTGCTCTTATCGCCAAATTCCGCAACAACGGCCAAACCTGCGTCTgcgc < 1:268263/132‑1 (MQ=255) tCAAGAAGGTTTCCCTGGAGCTGGGTGGCAACGCCCCGTTCATCGTGTTCGACGACGCCGACCTGGACAAGGCGGTCGAGGGCGCGATCATCTCCAAGTACCGTAACAACGGCCAGACCTGCGTCTGCGCCAACCg < 2:442516/136‑1 (MQ=255) tCAAGAAGGTTTCCCTGGAGCTGGGTGGCAACGCCCCGTTCATCGTGTTCGACGACGCCGACCTGGACAAGGCGGTCGAGGGCGCGATCATCTCCAAGTACCGTAACAACGGCCAGACCTGCGTCTGCGCCAACCg < 2:362394/136‑1 (MQ=255) tCAAGAAGCTTGGGCTTGAGCTTGGCGGCAACGCGCCTTTCATCGTGTTCGACGACGCCGATCTCGATGCCGCCGTCGAAGGTGCTCTTATCGCCAAATTCCGCAACAACGGCCAAACCTGCGTCTGCGCCAACCg < 2:94062/136‑1 (MQ=255) tCAAGAAGCTTGGGCTTGAGCTTGGCGGCAACGCGCCTTTCATCGTGTTCGACGACGCCGATCTCGATGCCGCCGTCGAAGGTGCTCTTATCGCCAAATTCCGCAACAACGGCCAAACCTGCGTCTGCGCCAACCg < 1:273245/136‑1 (MQ=255) cccTGGAGCTGGGTGGCAACGCCCCGTTCATCGTGTTCGACGACGCCGACCTGGACAAGGCGGTCGAGGGCGCGATCATCTCCAAGTACCGTAACAACGGCCAGACCTGCGTCTGCGCCAACCGTATCTACGTGCa > 1:354944/1‑136 (MQ=255) gcttgAGCTTGGCGGCAACGCGCCTTTCATCGTGTTCGACGACGCCGATCTCGATGCCGCCGTCGAAGGTGCTCTTATCGCCAAATTCCGCAACAACGGCCAAACCTGCGTCTGCGCCAACCGTCTCTATGTACAg < 1:439697/132‑1 (MQ=255) gcttgAGCTTGGCGGCAACGCGCCTTTCATCGTGTTCGACGACGCCGATCTCGATGCCGCCGTCGAAGGTGCTCTTATCGCCAAATTCCGCAACAACGGCCAAACCTGCGTCTGCGCCAACCGTCTCTATGTACAg > 2:401591/5‑136 (MQ=255) tGGAGCTGGGTGGCAACGCCCCGTTCATCGTGTTCGACGACGCCGACCTGGACAAGGCGGTCGAGGGCGCGATCATCTCCAAGTACCGTAACAACGGCCAGACCTGCGTCTGCGCCAACCGTATCTACGTGCAGGa < 2:472063/136‑1 (MQ=255) gAGCTTGGCGGCAACGCGCCTTTCATCGTGTTCGACGACGCCGATCTCGATGCCGCCGTCGAAGGTGCTCTTATCGCCAAATTCCGCAACAACGGCCAAACCTGCGTCTGCGCCAACCGTCTCTATGTACAGGACg < 2:490748/136‑1 (MQ=255) ggCAACGCGCCTTTCATCGTGTTCGACGACGCCGATCTCGATGCCGCCGTCGAAGGTGCTCTTATCGCCAAATTCCGCAACAACGGCCAAACCTGCGTCTGCGCCAACCGTCTCTATGTACAGGACGGCGTCTatg > 2:275773/1‑134 (MQ=255) ggCAACGCGCCTTTCATCGTGTTCGACGACGCCGATCTCGATGCCGCCGTCGAAGGTGCTCTTATCGCCAAATTCCGCAACAACGGCCAAACCTGCGTCTGCGCCAACCGTCTCTATGTACAGGACGGCGTCTatg > 2:248918/1‑134 (MQ=255) cGTTCATCGTGTTCGACGACGCCGACCTGGACAAGGCGGTCGAGGGCGCGATCATCTCCAAGTACCGTAACAACGGCCAGACCTGCGTCTGCGCCCACCGTATCTACGTGCAGGCCGGCGTCTACGACGCGTTCGc > 2:88661/1‑136 (MQ=255) cgacgaCGCCGATCTCGATGCCGCCGTCGAAGGTGCTCTTATCGCCAAATTCCGCAACAACGGCCAAACCTGCGTCTGCGCCAACCGTCTCTATGTACAGGACGGCGTCTATGATGCCTTTGCCGAGAAGCtctcc > 1:401379/1‑132 (MQ=255) gTCGAGGGCGCGATCATCTCCAAGTACCGTAACAACGGCCAGACCTGCGTCTGCGCCAACCGTATCTACGTGCAGGACGGCGTCTACGACGCGTTCGCCGAGAAGCTGGCCGCTGCAGTTGCCAAGCTGAAGATCg < 2:510322/136‑1 (MQ=255) aGGGCGCGATCATCTCCAAGTACCGTAACAACGGCCAGACCTGCGTCTGCGCCAACCGTATCTACGTGCAGGACGGCGTCTACGACGCGTTCGCCGAGAAGCTGGCCGCTGCAGTTGCCAAGCTGAAGATCGGTaa > 1:120496/1‑136 (MQ=255) aGGGCGCGATCATCTCCAAGTACCGTAACAACGGCCAGACCTGCGTCTGCGCCAACCGTATCTACGTGCAGGACGGCGTCTACGACGCGTTCGCCGAGAAGCTGGCCGCTGCAGTTGCCAAGCTGAAGATCGGTaa > 1:82102/1‑136 (MQ=255) aGGGCGCGATCATCTCCAAGTACCGTAACAACGGCCAGACCTGCGTCTGCGCCAACCGTATCTACGTGCAGGACGGCGTCTACGACGCGTTCGCCGAGAAGCTGGCCGCTGCAGTTGCCAAGCTGAAGATCGGTaa > 1:182090/1‑136 (MQ=255) ccAAGTACCGTAACAACGGCCAGACCTGCGTCTGCGCCAACCGTATCTACGTGCAGGACGGCGTCTACGACGCGTTCGCCGAGAAGCTGGCCGCTGCAGTTGCCAAGCTGAAGATCGGTAACGGCCTGGAAGAAgg < 2:484410/136‑1 (MQ=255) ccAGACCTGCGTCTGCGCCAACCGTATCTACGTGCAGGACGGCGTCTACGACGCGTTCGCCGAGAAGCTGGCCGCTGCAGTTGCCAAGCTGAAGATCGGTAACGGCCTGGAAGAAGGCACCACCACTGGCCCGCTg < 2:439623/136‑1 (MQ=255) | CGCCAAGGACATCAAGAAGGTTTCCCTGGAGCTGGGTGGCAACGCCCCGTTCATCGTGTTCGACGACGCCGACCTGGACAAGGCGGTCGAGGGCGCGATCATCTCCAAGTACCGTAACAACGGCCAGACCTGCGTCTGCGCCAACCGTATCTACGTGCAGGACGGCGTCTACGACGCGTTCGCCGAGAAGCTGGCCGCTGCAGTTGCCAAGCTGAAGATCGGTAACGGCCTGGAAGAAGGCACCACCACTGGCCCGCTG > NC_002947/263984‑264242 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |