| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 264,140 | G→A | 37.5% | V297V (GTG→GTA) | gabD‑I → | succinate‑semialdehyde dehydrogenase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 264,140 | 0 | G | A | 37.5% | 9.2 / 12.2 | 16 | V297V (GTG→GTA) | gabD‑I | succinate‑semialdehyde dehydrogenase |
| Reads supporting (aligned to +/- strand): ref base G (5/5); new base A (4/2); total (9/7) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.33e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.65e-01 | |||||||||||
CCCTGGAGCTGGGTGGCAACGCCCCGTTCATCGTGTTCGACGACGCCGACCTGGACAAGGCGGTCGAGGGCGCGATCATCTCCAAGTACCGTAACAACGGCCAGACCTGCGTCTGCGCCAACCGTATCTACGTGCAGGACGGCGTCTACGACGCGTTCGCCGAGAAGCTGGCCGCTGCAGTTGCCAAGCTGAAGATCGGTAACGGCCTGGAAGAAGGCACCACCACTGGCCCGCTGATCGATGGCAAG > NC_002947/264007‑264254 | cccTGGAGCTGGGTGGCAACGCCCCGTTCATCGTGTTCGACGACGCCGACCTGGACAAGGCGGTCGAGGGCGCGATCATCTCCAAGTACCGTAACAACGGCCAGACCTGCGTCTGCGCCAACCGTATCTACGTGCa > 1:354944/1‑136 (MQ=255) gcttgAGCTTGGCGGCAACGCGCCTTTCATCGTGTTCGACGACGCCGATCTCGATGCCGCCGTCGAAGGTGCTCTTATCGCCAAATTCCGCAACAACGGCCAAACCTGCGTCTGCGCCAACCGTCTCTATGTACAg < 1:439697/132‑1 (MQ=255) gcttgAGCTTGGCGGCAACGCGCCTTTCATCGTGTTCGACGACGCCGATCTCGATGCCGCCGTCGAAGGTGCTCTTATCGCCAAATTCCGCAACAACGGCCAAACCTGCGTCTGCGCCAACCGTCTCTATGTACAg > 2:401591/5‑136 (MQ=255) tGGAGCTGGGTGGCAACGCCCCGTTCATCGTGTTCGACGACGCCGACCTGGACAAGGCGGTCGAGGGCGCGATCATCTCCAAGTACCGTAACAACGGCCAGACCTGCGTCTGCGCCAACCGTATCTACGTGCAGGa < 2:472063/136‑1 (MQ=255) gAGCTTGGCGGCAACGCGCCTTTCATCGTGTTCGACGACGCCGATCTCGATGCCGCCGTCGAAGGTGCTCTTATCGCCAAATTCCGCAACAACGGCCAAACCTGCGTCTGCGCCAACCGTCTCTATGTACAGGACg < 2:490748/136‑1 (MQ=255) ggCAACGCGCCTTTCATCGTGTTCGACGACGCCGATCTCGATGCCGCCGTCGAAGGTGCTCTTATCGCCAAATTCCGCAACAACGGCCAAACCTGCGTCTGCGCCAACCGTCTCTATGTACAGGACGGCGTCTatg > 2:248918/1‑134 (MQ=255) ggCAACGCGCCTTTCATCGTGTTCGACGACGCCGATCTCGATGCCGCCGTCGAAGGTGCTCTTATCGCCAAATTCCGCAACAACGGCCAAACCTGCGTCTGCGCCAACCGTCTCTATGTACAGGACGGCGTCTatg > 2:275773/1‑134 (MQ=255) cGTTCATCGTGTTCGACGACGCCGACCTGGACAAGGCGGTCGAGGGCGCGATCATCTCCAAGTACCGTAACAACGGCCAGACCTGCGTCTGCGCCCACCGTATCTACGTGCAGGCCGGCGTCTACGACGCGTTCGc > 2:88661/1‑136 (MQ=255) cgacgaCGCCGATCTCGATGCCGCCGTCGAAGGTGCTCTTATCGCCAAATTCCGCAACAACGGCCAAACCTGCGTCTGCGCCAACCGTCTCTATGTACAGGACGGCGTCTATGATGCCTTTGCCGAGAAGCtctcc > 1:401379/1‑132 (MQ=255) gTCGAGGGCGCGATCATCTCCAAGTACCGTAACAACGGCCAGACCTGCGTCTGCGCCAACCGTATCTACGTGCAGGACGGCGTCTACGACGCGTTCGCCGAGAAGCTGGCCGCTGCAGTTGCCAAGCTGAAGATCg < 2:510322/136‑1 (MQ=255) aGGGCGCGATCATCTCCAAGTACCGTAACAACGGCCAGACCTGCGTCTGCGCCAACCGTATCTACGTGCAGGACGGCGTCTACGACGCGTTCGCCGAGAAGCTGGCCGCTGCAGTTGCCAAGCTGAAGATCGGTaa > 1:120496/1‑136 (MQ=255) aGGGCGCGATCATCTCCAAGTACCGTAACAACGGCCAGACCTGCGTCTGCGCCAACCGTATCTACGTGCAGGACGGCGTCTACGACGCGTTCGCCGAGAAGCTGGCCGCTGCAGTTGCCAAGCTGAAGATCGGTaa > 1:182090/1‑136 (MQ=255) aGGGCGCGATCATCTCCAAGTACCGTAACAACGGCCAGACCTGCGTCTGCGCCAACCGTATCTACGTGCAGGACGGCGTCTACGACGCGTTCGCCGAGAAGCTGGCCGCTGCAGTTGCCAAGCTGAAGATCGGTaa > 1:82102/1‑136 (MQ=255) ccAAGTACCGTAACAACGGCCAGACCTGCGTCTGCGCCAACCGTATCTACGTGCAGGACGGCGTCTACGACGCGTTCGCCGAGAAGCTGGCCGCTGCAGTTGCCAAGCTGAAGATCGGTAACGGCCTGGAAGAAgg < 2:484410/136‑1 (MQ=255) ccAGACCTGCGTCTGCGCCAACCGTATCTACGTGCAGGACGGCGTCTACGACGCGTTCGCCGAGAAGCTGGCCGCTGCAGTTGCCAAGCTGAAGATCGGTAACGGCCTGGAAGAAGGCACCACCACTGGCCCGCTg < 2:439623/136‑1 (MQ=255) cTGCGCCAACCGTATCTACGTGCAGGACGGCGTCTACGACGCGTTCGCCGAGAAGCTGGCCGCTGCAGTTGCCAAGCTGAAGATCGGTAACGGCCTGGAAGAAGGCACCACCACTGGCCCGCTGATCGATGGCAAg < 2:182090/136‑1 (MQ=255) | CCCTGGAGCTGGGTGGCAACGCCCCGTTCATCGTGTTCGACGACGCCGACCTGGACAAGGCGGTCGAGGGCGCGATCATCTCCAAGTACCGTAACAACGGCCAGACCTGCGTCTGCGCCAACCGTATCTACGTGCAGGACGGCGTCTACGACGCGTTCGCCGAGAAGCTGGCCGCTGCAGTTGCCAAGCTGAAGATCGGTAACGGCCTGGAAGAAGGCACCACCACTGGCCCGCTGATCGATGGCAAG > NC_002947/264007‑264254 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |