Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 459,290 T→A 12.6% T190S (ACC→TCC)  pqqB ← coenzyme PQQ synthesis protein B

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_002947459,2900TA12.6% 74.9 / 5.1 32T190S (ACC→TCC) pqqBcoenzyme PQQ synthesis protein B
Reads supporting (aligned to +/- strand):  ref base T (15/12);  new base A (2/2);  total (18/14)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.58e-01

ACGCCACGGCGCTGCATTTCGTCGTCTTCCCACAGGGTGCCGTCGACCAGCAGGCAGTCGGCACCGTGCATCATCGCCAGCAGCTTTTCGTCTACCTGGCCCAGGCCCGGGGCGTAGAACAGCTTGCCGCCGGTGCGGGTGTCTTCGACCATCAAACCAAGGTTATCGCCCGGGTGCGGGTCGAAGCGGTGTGGCGAATAAGGCGGCGCGGCGCTGCGCAGGGGGAAGGGAGTGAACTTGAGGTTGGGGCAGGCGTCGA  >  NC_002947/459157‑459415
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acgccacgGCGCTGCATTTCGTCGTCTTCCCACAGGGTGCCGTCGACCAGCAGGCAGTCGGCACCGTGCATCATCGCCAGCAGCTTTTCGTCTACCTGGCCCAGGCCCGGGGCGTAGAACAGCTTGCCGCCGGTGc                                                                                                                             <  1:363305/136‑1 (MQ=255)
  gccacgGCGCTGCATTTCGTCGTCTTCCCACAGGGTGCCGTCGACCAGCAGGCAGTCGGCACCGTGCATCATCGCCAGCAGCTTTTCGTCTACCTGGCCCAGGCCCGGGGCGTAGAACAGCTTGCCGCCGGTGCgg                                                                                                                           >  2:46164/1‑136 (MQ=255)
  gccacgGCGCTGCATTTCGTCGTCTTCCCACAGGGTGCCGTCGACCAGCAGGCAGTCGGCACCGTGCATCATCGCCAGCAGCTTTTCGTCTACCTGGCCCAGGCCCGGGGCGTAGAACAGCTTGCCGCCGGGGCgg                                                                                                                           >  2:90633/1‑136 (MQ=255)
  gccacgGCGCTGCATTTCGTCGTCTTCCCACAGGGTGCCGTCGACCAGCAGGCAGTCGGCACCGTGCATCATCGCCAGCAGCTTTTCGTCTACCTGGCCCAGGCCCGGGGCGTAGAACAGCTTGCCGCCGCTGCgg                                                                                                                           >  2:133458/1‑136 (MQ=255)
               aTTTCGTCGTCTTCCCACAGGGTGCCGTCGACCAGCAGGCAGTCGGCACCGTGCATCATCGCCAGCAGCTTTTCGTCTACCTGGCCCAGGCCCGGGGCGTAGAACAGCTTGCCGCCGGTGCGGGTGTCTTCGACCa                                                                                                              >  2:463277/1‑136 (MQ=255)
               aTTTCGTCGTCTTCCCACAGGGTGCCGTCGACCAGCAGGCAGTCGGCACCGTGCATCATCGCCAGCAGCTTTTCGTCTACCTGGCCCAGGCCCGGGGCGTAGAACAGCTTGCCGCCGGTGCGGGTGTCTTCGACCa                                                                                                              >  1:154557/1‑136 (MQ=255)
                 ttCGTCGTCTTCCCACAGGGTGCCGTCGACCAGCAGGCAGTCGGCACCGTGCATCATCGCCAGCAGCTTTTCGTCTACCTGGCCCAGGCCCGGGGCGTAGAACAGCTTGCCGCCGGTGCGGGTGTCTTCGACCATc                                                                                                            >  1:67103/1‑136 (MQ=255)
                           tCCCACAGGGTGCCGTCGACCAGCAGGCAGTCGGCACCGTGCATCATCGCCAGCAGCTTTTCGTCTACCTGGCCCAGGCCCGGGGCGTAGAACAGCTTGCCGCCGGTGCGGGTGTCTTCGACCATCAAACCAAGGt                                                                                                  <  1:462620/136‑1 (MQ=255)
                           acccACAGGGTGCCGTCGACCAGCAGGCAGTCGGCACCGTGCATCATCGCCAGCAGCTTTTCGTCTACCTGGCCCAGGCCCGGGGCGTAGAACAGCTTGCCGCCGGTGCGGGTGTCTTCGACCATCAAACCAAGGt                                                                                                  <  1:85233/135‑1 (MQ=255)
                                       ccGTCGACCAGCAGGCAGTCGGCACCGTGCATCATCGCCAGCAGCTTTTCGTCTACCTGGCCCAGGCCCGGGGCGTAGAACAGCTTGCCGCCGGTGCGGGTGTCTTCGACCATCAAACCAAGGTTATCGCCCGGGt                                                                                      >  1:197296/1‑136 (MQ=255)
                                       ccGTCGACCAGCAGGCAGTCGGCACCGTGCATCATCGCCAGCAGCTTTTCGTCTACCTGGCCCAGGCCCGGGGCGTAGAACAGCTTGCCGCCGGTGCGGGTGTCTTCGACCATCAAACCAAGGTTATCGCCCGGGt                                                                                      >  1:473502/1‑136 (MQ=255)
                                         gTCGACCAGCAGGCAGTCGGCACCGTGCATCATCGCCAGCAGCTTTTCGTCTACCTGGCCCAGGCCCGGGGCGTAGAACAGCTTGCCGCCGGTGCGGGTGTCTTCGACCATCAAACCAAGGTTATCGCCCGGGTGc                                                                                    <  1:373945/136‑1 (MQ=255)
                                             aCCAGCAGGCAGTCGGCACCGTGCATCATCGCCAGCAGCTTTTCGTCTACCTGGCCCAGGCCCGGGGCGTAGAACAGCTTGCCGCCGGAGCGGGTGTCTTCGACCATCAAACCAAGGTTATCGCCCGGGTGCGGGt                                                                                >  1:347455/1‑136 (MQ=255)
                                             aCCAGCAGGCAGTCGGCACCGTGCATCATCGCCAGCAGCATTTCGTCTACCTGGCCCAGGCCCGGGGCGTAGAACAGCTTGCCGCCGGAGCGGGTGTCTTCGACCATCAAACCAAGGTTATCGCCCGGGTGCgggg                                                                                >  1:350005/1‑135 (MQ=255)
                                                agcagGCAGTCGGCACCGTGCATCATCGCCAGCAGCTTTTCGTCTACCTGGCCCAGGCCCGGGGCGTAGAACAGCTTGCCGCCGGTGCGGGTGTCTTCGACCATCAAACCAAGGTTATCGCCCGGGTGCGGGTCGa                                                                             >  2:255403/1‑136 (MQ=255)
                                                agcagGCAGTCGGCACCGTGCATCATCGCCAGCAGCTTTTCGTCTACCTGGCCCAGGCCCGGGGCGTAGAACAGCTTGCCGCCGGTGCGGGTGTCTTCGACCATCAAACCAAGGTTATCGCCCGGGTGCGGGTCGa                                                                             >  2:286882/1‑136 (MQ=255)
                                                                cGTGCATCATCGCCAGCAGCTTTTCGTCTACCTGGCCCAGGCCCGGGGCGTAGAACAGCTTGCCGCCGGTGCGGGTGTCTTCGACCATCAAACCAAGGTTATCGCCCGGGTGCGGGTCGAAGCGGTGTGGCGAATa                                                             <  2:234253/136‑1 (MQ=255)
                                                                          ggCCAGCAGCTTTTCGTCTACCTGGCCCAGGCCCGGGGCGTAGAACAGCTTGCCGCCGGTGCGGGTGTCTTCGACCATCAAACCAAGGTTATCGCCCGGGTGCGGGTCGAAGCGGTGTGGCGAATAAGgcggcgcg                                                   <  1:425541/135‑1 (MQ=255)
                                                                          cGCCAGCAGCTTTTCGTCTACCTGGCCCAGGCCCGGGGCGTAGAACAGCTTGCCGCCGGTGCGGGTGTCTTCGACCATCAAACCAAGGTTATCGCCCGGGTGCGGGTCGAAGCGGTGTGGCGAATAAGgcggcgcg                                                   <  1:317308/136‑1 (MQ=255)
                                                                           gCCAGCAGCTTTTCGTCTACCTGGCCCAGGCCCGGGGCGTAGAACAGCTTGCCGCCGGTGCGGGTGTCTTCGACCATCAAACCAAGGTTATCGCCCGGGTGCGGGTCGAAGCGGTGTGGCGAATAAGgcggcgcgg                                                  <  2:36134/136‑1 (MQ=255)
                                                                           gCCAGCAGCTTTTCGTCTACCTGGCCCAGGCCCGGGGCGTAGAACAGCTTGCCGCCGGTGCGGGTGTCTTCGACCATCAAACCAAGGTTATCGCCCGGGTGCGGGTCGAAGCGGTGTGGCGAATAAGgcggcgcgg                                                  <  2:235427/136‑1 (MQ=255)
                                                                            ccAGCAGCTTTTCGTCTACCTGGCCCAGGCCCGGGGCGTAGAACAGCTTGCCGCCGGTGCGGGTGTCTTCGACCATCAAACCAAGGTTATCGCCCGGGTGCGGGTCGAAGCGGTGTGGCGAATAAGgcggcgcggc                                                 >  1:475295/1‑136 (MQ=255)
                                                                                cagcTTTTCGTCTACCTGGCCCAGGCCCGGGGCGTAGAACAGCTTGCCGCCGGTGCGGGTGTCTTCGACCATCAAACCAAGGTTATCGCCCGGGTGCGGGTCGAAGCGGTGTGGCGAATAAGGCGGCGCGGCGCTg                                             <  1:286882/136‑1 (MQ=255)
                                                                                      ttCGTCTACCTGGCCCAGGCCCGGGGCGTAGAACAGCTTGCCGCCGGTGCGGGTGTCTTCGACCATCAAACCAAGGTTATCGCCCGGGTGCGGGTCGAAGCGGTGTGGCGAATAAGGCGGCGCGGCGCTGCGCAgg                                       >  2:464847/1‑136 (MQ=255)
                                                                                      ttCGTCTACCTGGCCCAGGCCCGGGGCGTAGAACAGCTTGCCGCCGGTGCGGGTGTCTTCGACCAGCACACCAAGGTTATCGCCCGGGTGCGGGTCGAAGCGGTGTGGCGAATAAGGCGGCGCGGCGCTGCGCAgg                                       >  2:413003/1‑136 (MQ=255)
                                                                                                              ggCGTAGAACAGCTTGCCGCCGGTGCGGGTGTCTTCGACCATCAAACCAAGGTTATCGCCCGGGTGCGGGTCGAAGCGGTGTGGCGAATAAGGCGGCGCGGCGCTGCGCAGGGGGAAGGGAGTGAACTTGAGGTTg               <  2:197296/136‑1 (MQ=255)
                                                                                                              ggCGTAGAACAGCTTGCCGCCGGTGCGGGTGTCTTCGACCATCAAACCAAGGTTATCGCCCGGGTGCGGGTCGAAGCGGTGTGGCGAATAAGGCGGCGCGGCGCTGCGCAGGGGGAAGGGAGTGAACTTGAGGTTg               <  2:473502/136‑1 (MQ=255)
                                                                                                                cGTAGAACAGCTTGCCGCCGGTGCGGGTGTCTTCGACCATCAAACCAAGGTTATCGCCCGGGTGCGGGTCGAAGCGGTGTGGCGAATAAGGCGGCGCGGCGCTGCGCAGGGGGAAGGGAGTGAACTTGAGGTTggg             >  2:335882/1‑136 (MQ=255)
                                                                                                                     aaCAGCTTGCCGCCGGTGCGGGTGTCTTCGACCATCAAACCAAGGTTATCGCCCGGGTGCGGGTCGAAGCGGTGTGGCGAATAAGGCGGCGCGGCGCTGCGCAGGGGGAAGGGAGTGAACTTGAGGTTGGGGCAgg        >  1:384527/1‑136 (MQ=255)
                                                                                                                     aaCAGCTTGCCGCCGGTGCGGGTGTCTTCGACCATCAAACCAAGGTTATCGCCCGGGTGCGGGTCGAAGCGGTGTGGCGAATAAGGCGGCGCGGCGCTGCGCAGGGGGAAGGGAGTGAACTTGAGGTTGGGGCAgg        >  1:490726/1‑136 (MQ=255)
                                                                                                                           ttGCCGCCGGAGCGGGTGTCTTCGACCATCAAACCAAGGTTATCGCCCGGGTGCGGGTCGATGCGGTGTGGCGAATAAGGCGGCGCGGCGCTGCGCAGGGGGAAGGGAGTGAACTTGAGGTTGGGGCAGGCGTCGa  <  2:350005/136‑1 (MQ=255)
                                                                                                                           ttGCCGCCGGAGCGGGTGTCTTCGACCATCAAACCAAGGTTATCGCCCGGGTGCGGGTCGAAGCGGTGTGGCGAATAAGGCGGCGCGGCGCTGCGCAGGGGGAAGGGAGTGAACTTGAGGTTGGGGCAGGCGTCGa  <  2:347455/136‑1 (MQ=255)
                                                                                                                                     |                                                                                                                             
ACGCCACGGCGCTGCATTTCGTCGTCTTCCCACAGGGTGCCGTCGACCAGCAGGCAGTCGGCACCGTGCATCATCGCCAGCAGCTTTTCGTCTACCTGGCCCAGGCCCGGGGCGTAGAACAGCTTGCCGCCGGTGCGGGTGTCTTCGACCATCAAACCAAGGTTATCGCCCGGGTGCGGGTCGAAGCGGTGTGGCGAATAAGGCGGCGCGGCGCTGCGCAGGGGGAAGGGAGTGAACTTGAGGTTGGGGCAGGCGTCGA  >  NC_002947/459157‑459415

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑