Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	732,534	T→C	20.8%	L691L (TTG→CTG)	clpB →	chaperone protein ClpB

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	732,534	0	T	C	20.8%	40.8 / 8.6	24	L691L (TTG→CTG)	clpB	chaperone protein ClpB
Reads supporting (aligned to +/- strand):  ref base T (12/7);  new base C (2/3);  total (14/10)
Fisher's exact test for biased strand distribution p-value = 6.15e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.04e-01

ATCGGTGCACCACCAGGCTATGTAGGGTATGAAGAGGGCGGTTACCTGACCGAGGCCGTGCGGCGCAAGCCTTACTCGGTGGTGCTGCTGGACGAGGTGGAAAAAGCCCACCCGGATGTGTTCAACGTGCTGTTGCAGGTGCTGGAAGACGGCCGCCTGACCGACAGCCACGGGCGCACCGTGGACTTCCGCAACACCGTGATCGTGATGACCTCCAACCTGGGCTCGGCGCAGATTCAGG  >  NC_002947/732402‑732642                                                                                                                                     |                                                                                                             attggTGCACCTCCCGGTTATGTCGGCTATGAAGAGGGTGGCGTTCTGACGGAAGCTGTGCGTCGCAGACCATATCAGGTGATCCTGTTCGATGAGATCGAAAAGGCGCATCCGGATGTGTTCAACGtgctgctgc                                                                                                           <  2:508314/133‑1 (MQ=255)         accaccAGGCTATGTAGGGTATGAAGAGGGTGGTTACCTGACCGAGGCCGTGCGGCGCAAGCCTTACTCGGTGGTGCTGCTGGACGAGGTGGAAAAAGCCCACCCGGATGTGTTCAACGTGCTGTTGCAGGTGCTg                                                                                                   >  2:112659/1‑136 (MQ=255)         accaccAGGCTATGTAGGGTATGAAGAGGGCGGTTACCTGACCGAGGCCGTGCGGCGCAAGCCTTACTCGGTGGTGCTGCTGGACGAGGTGGAAAAAGCCCACCCGGATGTGTTCAACGTGCTGTTGCAGGTGCTg                                                                                                   >  2:394523/1‑136 (MQ=255)         accaccAGGCTATGTAGGGTATGAAGAGGGCGGTTACCTGACCGAGGCCGTGCGGCGCAAGCCTTACTCGGTGGTGCTGCTGGACGAGGTGGAAAAAGCCCACCCGGATGTGTTCAACGTGCTGTTGCAGGTGCTg                                                                                                   >  2:110769/1‑136 (MQ=255)                   tATGTAGGGTATGAAGAGGGCGGTTACCTGACCGAGGCCGTGCGGCGCAAGCCTTACTCGGTGGTGCTGCTGGACGAGGTGGAAAAAGCCCACCCGGATGTGTTCAACGTGCTGTTGCAGGTGCTGGAAGACGgcc                                                                                         >  2:276681/1‑136 (MQ=255)                       tcggctATGAAGAGGGTGGCGTTCTGACGGAAGCTGTGCGTCGCAGACCATATCAGGTGATCCTGTTCGATGAGATCGAAAAGGCGCATCCGGATGTGTTCAACGTGCTGCTGCAGGTTCTTGATGACGGTCGTCt                                                                                     >  1:374781/6‑136 (MQ=255)                        aGGGTATGAAGAGGGCGGTTACCTGACCGAGGCCGTGCGGCGCAAGCCTTACTCGGTGGTGCTGCTGGACGAGGTGGAAAAAGCCCACCCGGATGTGTTCAACGTGCTGTTGCAGGTGCTGGAAGACGGCCGCCTg                                                                                    >  1:134095/1‑136 (MQ=255)                        aGGGTATGAAGAGGGCGGTTACCTGACCGAGGCCGTGCGGCGCAAGCCTTACTCGGTGGTGCTGCTGGACGAGGTGGAAAAAGCCCACCCGGATGTGTTCAACGTGCTGTTGCAGGTGCTGGAAGACGGCCGCCTg                                                                                    >  2:281694/1‑136 (MQ=255)                         ggctATGAAGAGGGTGGCGTTCTGACGGAAGCTGTGCGTCGCAGACCATATCAGGTGATCCTGTTCGATGAGATCGAAAAGGCGCATCCGGATGTGTTCAACGTGCTGCTGCAGGTTCTTGATGACGGTCGTCTGa                                                                                   <  2:502383/133‑1 (MQ=255)                          ggTATGAAGAGGGCGGTTACCTGACCGAGGCCGTGCGGCGCAAGCCTTACTCGGTGGTGCTGCTGGACGAGGTGGAAAAAGCCCACCCGGATGTGTTCAACGTGCTGTTGCAGGTGCTGGAAGACGGCCGCCTGAc                                                                                  <  1:110769/136‑1 (MQ=255)                          ggTATGAAGAGGGCGGTTACCTGACCGAGGCCGTGCGGCGCAAGCCTTACTCGGTGGTGCTGCTGGACGAGGTGGAAAAAGCCCACCCGGATGTGGTCACCGGGCTGTTGCAGGTGCTGGAAGACGGCCGCCTGAc                                                                                  <  1:276681/136‑1 (MQ=255)                                aaGAGGGCGGTTACCTGACCGAGGCCGTGCGGCGCAAGCCTTACTCGGTGGTGCTGCTGGACGAGGTGGAAAAAGCCCACCCGGATGTGTTCAACGTGCTGTTGCAGGTGCTGGAAGACGGCCGCCTGACCGACAg                                                                            <  2:511257/136‑1 (MQ=255)                                aaGAGGGCGGTTACCTGACCGAGGCCGTGCGGCGCAAGCCTTACTCGGTGGTGCTGCTGGACGAGGTGGAAAAAGCCCACCCGGATGTGTTCAACGTGCTGCTGCAGGTGCTGGAGGACGGCCGCCTGACCGACAg                                                                            <  2:335251/136‑1 (MQ=255)                                        ggTTACCTGACCGAGGCCGTGCGGCGCAAGCCTTACTCGGTGGTGCTGCTGGACGAGGTGGAAAAAGCCCACCCGGATGTGTTCAACGTGCTGTTGCAGGTGCTGGAAGACGGCCGCCTGACCGACAGCCACGGgc                                                                    >  1:426130/1‑136 (MQ=255)                                        ggTTACCTGACCGAGGCCGTGCGGCGCAAGCCTTACTCGGTGGTGCTGCTGGACGAGGTGGAAAAAGCCCACCCGGATGTGTTCAACGTGCTGTTGCAGGTGCTGGAAGACGGCCGCCTGACCGACAGCCACGGgc                                                                    >  1:474384/1‑136 (MQ=255)                                                         cGTGCGGCGCAAGCCTTACTCGGTGGTGCTGCTGGACGAGGTGGAAAAAGCCCACCCGGATGTGTTCAACGTGCTGTTGCAGGTGCTGGAAGACGGCCGCCTGACCGACAGCCACGGGCGCACCGTGGACTTCCGc                                                   >  1:186546/1‑136 (MQ=255)                                                              ggcgCAAGCCTTACTCGGTGGTGCTGCTGGACGAGGTGGAAAAAGCCCACCCGGATGTGTTCAACGTGCTGTTGCAGGTGCTGGAAGACGGCCGCCTGACCGACAGCCACGGGCGCACCGTGGACTTCCGCAacac                                              >  1:412922/1‑136 (MQ=255)                                                                            tgggtggtGCTGCTGGACGTGGTTGAAAAAGCCTACCCGTATGTGTTCAACGTGCTTTTGCAGGTGCTGGAAGACGGCCGCCTGACCGACAGCCACGGGCGCACCGTGGACTTCCGCAACACCGTGATCGtgttga                                <  2:412922/134‑1 (MQ=255)                                                                            tCGGTTGTGCTGATGGACGAGGTGGAAAAAGCCCACCCGGATGTGTTCAACGTGCTGTTGCAGGTGCTGGAAGACGGCCGCCTGACCGACAGCCACGGGCGCACCGTGGACTTCCGCAACACCGTGATCGtgatga                                <  1:486396/136‑1 (MQ=255)                                                                            caggtgatCCTGTTCGATGAGATCGAAAAGGCGCATCCGGATGTGTTCAACGTGCTGCTGCAGGTTCTTGATGACGGTCGTCTGa                                                                                   <  1:470901/83‑1 (MQ=255)                                                                            caggtgatCCTGTTCGATGAGATCGAAAAGGCGCATCCGGATGTGTTCAACGTGCTGCTGCAGGTTCTTGATGACGGTCGTCTGa                                                                                   >  2:470901/3‑85 (MQ=255)                                                                                  gtgCTGCTGGACGAGGTGGAAAAAGCCCACCCGGATGTGTTCAACGTGCTGTTGCAGGTGCTGGAAGACGGCCGCCTGACCGACAGCCACGGGCGCACCGTGGACTTCCGCAACACCGTGATCGTGATGACCTCCa                          >  1:447534/1‑136 (MQ=255)                                                                                   tgctgcTGGACGAGGTGGAAAAAGCCCACCCGGATGTGTTCAACGTGCTGTTGCAGGTGCTGGAAGACGGCCGCCTGACCGACAGCCACGGGCGCACCGTGGACTTCCGCAACACCGTGATCGTGATGACCTCCaa                         >  2:459689/1‑136 (MQ=255)                                                                                               aGGTGGAAAAAGCCCACCCGGATGTGTTCAACGTGCTGTTGCAGGTGCTGGAAGACGGCCGCCTGACCGACAGCCACGGGCGCACCGTGGACTTCCGCAACACCGTGATCGTGATGACCTCCAACCTGGGCTCGgc             <  2:134095/136‑1 (MQ=255)                                                                                                          gCCCACCCGGATGTGTTCAACGTGCTGTTGCAGGTGCTGGAAGACGGCCGCCTGACCGACAGCCACGGGCGCACCGTGGACTTCCGCAACACCGTGATCGTGATGACCTCCAACCTGGGCTCGGCGCAGATTCAgg  <  2:426130/136‑1 (MQ=255)                                                                                                                                     |                                                                                                             ATCGGTGCACCACCAGGCTATGTAGGGTATGAAGAGGGCGGTTACCTGACCGAGGCCGTGCGGCGCAAGCCTTACTCGGTGGTGCTGCTGGACGAGGTGGAAAAAGCCCACCCGGATGTGTTCAACGTGCTGTTGCAGGTGCTGGAAGACGGCCGCCTGACCGACAGCCACGGGCGCACCGTGGACTTCCGCAACACCGTGATCGTGATGACCTCCAACCTGGGCTCGGCGCAGATTCAGG  >  NC_002947/732402‑732642

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑