Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	230,432	G→A	23.4%	V31V (GTG→GTA)	PP_0179 →	putative efflux transporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	230,432	0	G	A	23.4%	30.9 / 2.1	17	V31V (GTG→GTA)	PP_0179	putative efflux transporter
Reads supporting (aligned to +/- strand):  ref base G (11/2);  new base A (2/2);  total (13/4)
Fisher's exact test for biased strand distribution p-value = 2.19e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

TGCGACGGTGTCGATCATCGAGGGCCAGCGCCCATGAAACAGCTGATCCTGGCTGGCCTGTGCCTGTCGCTGGGGGCCTGCATGATGGTGGGGCCCGACTATGAGGTGCCTGGGGATGCGGCCGTGCAACGCAATGACCTGAACGGCCCGCTGCGCCAGGATGCCGACAGCGTCGTGTCAGCGCCGGTCCCCGAGGACTGGTGGCAGCTGTATCAGGATCAGCGGCTGAACGAGCTGGTGCGTCAGGCGCTGAGTGC  >  NC_002947/230307‑230563                                                                                                                              |                                                                                                                                    tGCGACGGTGTCGATCATCGAGGGCCAGCGCCCATGAAACAGCTGATCCTGGCTGGCCTGTGACGGTCGCTGGGGGCCTGCATGATGGTGGGGCCCGACTATGAGGTGCCTGGGGATGCGGCCGTGCCACGCACTg                                                                                                                           >  2:430724/1‑136 (MQ=255)            cGATCATCGAGGGCCAGCGCCCATGAAACAGCTGATCCTGGCTGGCCTGTGCCTGTCGCTGGGGGCCTGCATGATGGTGGGGCCCGACTATGAGGTGCCTGGGGATGCGGCCGTGCAACGCAATGACCTGAACGGc                                                                                                                >  2:468517/1‑136 (MQ=255)                      gggCCAGCGCCCATGACACAGCTGATCCTGGCTGGCCTGTGCCTGTCGCTGGGGGCCGGCATGATGGTGGGGCCCGACTATGAGGTGCCTGGGGATGCGGCCGTGCAACGCAATGACCTGAACGGCCCGCTGCGcc                                                                                                      <  2:311819/136‑1 (MQ=255)                                                                ctgtCGCTGGGGGCCTGCATGATGGTGGGGCCCGACTATGAGGTGCCTGGGGATGCGGCCGTGCAACGCAATGACCTGAACGGCCCGCTGCGCCAGGATGCCGACAGCGTCGTGTCAGCGCCGGTCCCCGAGGAcg                                                            >  1:497821/1‑135 (MQ=255)                                                                     gCTGGGGGCCTGCATGATGGTGGGGCCCGACTATGAGGTGCCTGGGGATGCGGCCGTGCAACGCAATGACCTGAACGGCCCGCTGCGCCAGGATGCCGACAGCGTCGTGTCAGCGCCGGTCCCCGAGGACtggtgg                                                       >  1:83428/1‑136 (MQ=255)                                                                     gCTGGGGGCCTGCATGATGGTGGGGCCCGACTATGAGGTGCCTGGGGATGCGGCCGTGCAACGAAATGACCTGAACGGCCCGCTGCGCCAGGATGCCGACAGCGTCGTGTCAGCGCCGGTCCCCGAGGCCtggtgg                                                       >  2:459410/1‑136 (MQ=255)                                                                      cTGGGGGCCTGCATGATGGTGGGGCCCGACTATGAGGTGCCTGGGGATGCGGCCGTGCAACGCAATGACCTGAACGGCCCGCTGCGCCAGGATGCCGACAGCGTCGTGTCAGCGCCGGTCCCCGAGGACTGGTGGc                                                      >  1:124141/1‑136 (MQ=255)                                                                                    gatgGTGGGGCCCGACTATGAGGTGCCTGGGGATGCGGCCGTGCAACGCAATGACCTGAACGGCCCGCTGCGCCAGGATGCCGACAGCGTCGTGTCAGCGCCGGTCCCCGAGGACTGGTGGCAGCTGTATCAGGAt                                        >  1:308356/1‑136 (MQ=255)                                                                                            ggCCCGACTATGAGGTGCCTGGGGATGCGGCCGTGCAACGCAATGACCTGAACGGCCCGCTGCGCCAGGATGCCGACAGCGTCGTGTCAGCGCCGCTCCCCGAGGACTGGTGGCAGCTGTATCAGGATCAGCGGCt                                >  2:252725/1‑136 (MQ=255)                                                                                              cccGACTATGAGGTGCCTGGGGATGCGGCCGTACAACGCAATGACCTGAACGGCCCGCTGCGCCAGGATGCCGACAGCGTCGTGTCAGCGCCGGTCCCCGAGGACtggt                                                         <  1:21243/109‑1 (MQ=255)                                                                                              cccGACTATGAGGTGCCTGGGGATGCGGCCGTACAACGCAATGACCTGAACGGCCCGCTGCGCCAGGATGCCGACAGCGTCGTGTCAGCGCCGGTCCCCGAGGACtggt                                                         <  1:23709/109‑1 (MQ=255)                                                                                              cccGACTATGAGGTGCCTGGGGATGCGGCCGTACAACGCAATGACCTGAACGGCCCGCTGCGCCAGGATGCCGACAGCGTCGTGTCAGCGCCGGTCCCCGAGGACtggt                                                         >  2:21243/1‑109 (MQ=255)                                                                                              cccGACTATGAGGTGCCTGGGGATGCGGCCGTACAACGCAATGACCTGAACGGCCCGCTGCGCCAGGATGCCGACAGCGTCGTGTCAGCGCCGGTCCCCGAGGACtggt                                                         >  2:23709/1‑109 (MQ=255)                                                                                                         ggTGCCTGGGGATGCGGCCGTGCAACGCAATGACCTGAACGGCCCGCTGCGCCAGGATGCCGACAGCGTCGTGTCAGCGCCGGTCCCCGAGGACTGGTGGCAGCTGTATCAGGATCAGCGGCTGAACGAGCTGGTg                   <  2:83428/136‑1 (MQ=255)                                                                                                           tGCCTGGGGATGCGGCCGTGCAACGCAATGACCTGAACGGCCCGCTGCGCCAGGATGCCGACAGCGTCGTGTCAGCGCCGGTCCCCGAGGACTGGTGGCAGCTGTATCAGGATCAGCCGCTGAACGAGCTGGTGCg                 >  1:382624/1‑136 (MQ=255)                                                                                                                ggggATGCGGCAGTGAAACTCAATGACCTGAACGGCCCGCTGAGCCAGGATGCCTAAAGAGGCGGGTCAGCGCACGTACCCGAGGACTTGTGGCCTCGGTATCAGGAGAAGCGGCGGAACGAGCGGGTGCGGCAgg            >  2:107744/1‑136 (MQ=255)                                                                                                                          ccGTGCAACGCAATGACCTGAACGGCCCGCTGCGCCAGGATGCCGACAGCGTCGTGTCAGCGCCGGTCCCCGAGGACTGGTGGCCGCTGTTTCAGGATCAGCGGCTGAACGAGCTGGTGCGTGAGGCGCGGAGTgg  >  1:296409/1‑135 (MQ=255)                                                                                                                              |                                                                                                                                    TGCGACGGTGTCGATCATCGAGGGCCAGCGCCCATGAAACAGCTGATCCTGGCTGGCCTGTGCCTGTCGCTGGGGGCCTGCATGATGGTGGGGCCCGACTATGAGGTGCCTGGGGATGCGGCCGTGCAACGCAATGACCTGAACGGCCCGCTGCGCCAGGATGCCGACAGCGTCGTGTCAGCGCCGGTCCCCGAGGACTGGTGGCAGCTGTATCAGGATCAGCGGCTGAACGAGCTGGTGCGTCAGGCGCTGAGTGC  >  NC_002947/230307‑230563

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑