| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 732,548 | A→T | 16.2% | E695D (GAA→GAT) | clpB → | chaperone protein ClpB |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 732,548 | 0 | A | T | 16.2% | 54.1 / 5.5 | 25 | E695D (GAA→GAT) | clpB | chaperone protein ClpB |
| Reads supporting (aligned to +/- strand): ref base A (9/11); new base T (2/2); total (11/14) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.49e-01 | |||||||||||
TATGTAGGGTATGAAGAGGGCGGTTACCTGACCGAGGCCGTGCGGCGCAAGCCTTACTCGGTGGTGCTGCTGGACGAGGTGGAAAAAGCCCACCCGGATGTGTTCAACGTGCTGTTGCAGGTGCTGGAAGACGGCCGCCTGACCGACAGCCACGGGCGCACCGTGGACTTCCGCAACACCGTGATCGTGATGACCTCCAACCTGGGCTCGGCGCAGATTCAGGAACTGGTGGGTGACCGCGAGGCACAGCGTGCGGCAGTG > NC_002947/732420‑732680 | tATGTAGGGTATGAAGAGGGCGGTTACCTGACCGAGGCCGTGCGGCGCAAGCCTTACTCGGTGGTGCTGCTGGACGAGGTGGAAAAAGCCCACCCGGATGTGTTCAACGTGCTGTTGCAGGTGCTGGAAGACGgcc > 2:276681/1‑136 (MQ=255) tcggctATGAAGAGGGTGGCGTTCTGACGGAAGCTGTGCGTCGCAGACCATATCAGGTGATCCTGTTCGATGAGATCGAAAAGGCGCATCCGGATGTGTTCAACGTGCTGCTGCAGGTTCTTGATGACGGTCGTCt > 1:374781/6‑136 (MQ=255) aGGGTATGAAGAGGGCGGTTACCTGACCGAGGCCGTGCGGCGCAAGCCTTACTCGGTGGTGCTGCTGGACGAGGTGGAAAAAGCCCACCCGGATGTGTTCAACGTGCTGTTGCAGGTGCTGGAAGACGGCCGCCTg > 1:134095/1‑136 (MQ=255) aGGGTATGAAGAGGGCGGTTACCTGACCGAGGCCGTGCGGCGCAAGCCTTACTCGGTGGTGCTGCTGGACGAGGTGGAAAAAGCCCACCCGGATGTGTTCAACGTGCTGTTGCAGGTGCTGGAAGACGGCCGCCTg > 2:281694/1‑136 (MQ=255) ggctATGAAGAGGGTGGCGTTCTGACGGAAGCTGTGCGTCGCAGACCATATCAGGTGATCCTGTTCGATGAGATCGAAAAGGCGCATCCGGATGTGTTCAACGTGCTGCTGCAGGTTCTTGATGACGGTCGTCTGa < 2:502383/133‑1 (MQ=255) ggTATGAAGAGGGCGGTTACCTGACCGAGGCCGTGCGGCGCAAGCCTTACTCGGTGGTGCTGCTGGACGAGGTGGAAAAAGCCCACCCGGATGTGTTCAACGTGCTGTTGCAGGTGCTGGAAGACGGCCGCCTGAc < 1:110769/136‑1 (MQ=255) ggTATGAAGAGGGCGGTTACCTGACCGAGGCCGTGCGGCGCAAGCCTTACTCGGTGGTGCTGCTGGACGAGGTGGAAAAAGCCCACCCGGATGTGGTCACCGGGCTGTTGCAGGTGCTGGAAGACGGCCGCCTGAc < 1:276681/136‑1 (MQ=255) aaGAGGGCGGTTACCTGACCGAGGCCGTGCGGCGCAAGCCTTACTCGGTGGTGCTGCTGGACGAGGTGGAAAAAGCCCACCCGGATGTGTTCAACGTGCTGTTGCAGGTGCTGGAAGACGGCCGCCTGACCGACAg < 2:511257/136‑1 (MQ=255) aaGAGGGCGGTTACCTGACCGAGGCCGTGCGGCGCAAGCCTTACTCGGTGGTGCTGCTGGACGAGGTGGAAAAAGCCCACCCGGATGTGTTCAACGTGCTGCTGCAGGTGCTGGAGGACGGCCGCCTGACCGACAg < 2:335251/136‑1 (MQ=255) ggTTACCTGACCGAGGCCGTGCGGCGCAAGCCTTACTCGGTGGTGCTGCTGGACGAGGTGGAAAAAGCCCACCCGGATGTGTTCAACGTGCTGTTGCAGGTGCTGGAAGACGGCCGCCTGACCGACAGCCACGGgc > 1:474384/1‑136 (MQ=255) ggTTACCTGACCGAGGCCGTGCGGCGCAAGCCTTACTCGGTGGTGCTGCTGGACGAGGTGGAAAAAGCCCACCCGGATGTGTTCAACGTGCTGTTGCAGGTGCTGGAAGACGGCCGCCTGACCGACAGCCACGGgc > 1:426130/1‑136 (MQ=255) cGTGCGGCGCAAGCCTTACTCGGTGGTGCTGCTGGACGAGGTGGAAAAAGCCCACCCGGATGTGTTCAACGTGCTGTTGCAGGTGCTGGAAGACGGCCGCCTGACCGACAGCCACGGGCGCACCGTGGACTTCCGc > 1:186546/1‑136 (MQ=255) ggcgCAAGCCTTACTCGGTGGTGCTGCTGGACGAGGTGGAAAAAGCCCACCCGGATGTGTTCAACGTGCTGTTGCAGGTGCTGGAAGACGGCCGCCTGACCGACAGCCACGGGCGCACCGTGGACTTCCGCAacac > 1:412922/1‑136 (MQ=255) tgggtggtGCTGCTGGACGTGGTTGAAAAAGCCTACCCGTATGTGTTCAACGTGCTTTTGCAGGTGCTGGAAGACGGCCGCCTGACCGACAGCCACGGGCGCACCGTGGACTTCCGCAACACCGTGATCGtgttga < 2:412922/134‑1 (MQ=255) tCGGTTGTGCTGATGGACGAGGTGGAAAAAGCCCACCCGGATGTGTTCAACGTGCTGTTGCAGGTGCTGGAAGACGGCCGCCTGACCGACAGCCACGGGCGCACCGTGGACTTCCGCAACACCGTGATCGtgatga < 1:486396/136‑1 (MQ=255) caggtgatCCTGTTCGATGAGATCGAAAAGGCGCATCCGGATGTGTTCAACGTGCTGCTGCAGGTTCTTGATGACGGTCGTCTGa < 1:470901/83‑1 (MQ=255) caggtgatCCTGTTCGATGAGATCGAAAAGGCGCATCCGGATGTGTTCAACGTGCTGCTGCAGGTTCTTGATGACGGTCGTCTGa > 2:470901/3‑85 (MQ=255) gtgCTGCTGGACGAGGTGGAAAAAGCCCACCCGGATGTGTTCAACGTGCTGTTGCAGGTGCTGGAAGACGGCCGCCTGACCGACAGCCACGGGCGCACCGTGGACTTCCGCAACACCGTGATCGTGATGACCTCCa > 1:447534/1‑136 (MQ=255) tgctgcTGGACGAGGTGGAAAAAGCCCACCCGGATGTGTTCAACGTGCTGTTGCAGGTGCTGGAAGACGGCCGCCTGACCGACAGCCACGGGCGCACCGTGGACTTCCGCAACACCGTGATCGTGATGACCTCCaa > 2:459689/1‑136 (MQ=255) aGGTGGAAAAAGCCCACCCGGATGTGTTCAACGTGCTGTTGCAGGTGCTGGAAGACGGCCGCCTGACCGACAGCCACGGGCGCACCGTGGACTTCCGCAACACCGTGATCGTGATGACCTCCAACCTGGGCTCGgc < 2:134095/136‑1 (MQ=255) gCCCACCCGGATGTGTTCAACGTGCTGTTGCAGGTGCTGGAAGACGGCCGCCTGACCGACAGCCACGGGCGCACCGTGGACTTCCGCAACACCGTGATCGTGATGACCTCCAACCTGGGCTCGGCGCAGATTCAgg < 2:426130/136‑1 (MQ=255) gCTGGAAGACGGCCGCCTGACCGACAGCCACGGGCGCACCGTGGACTTCCGCAACACCGTGATCGTGATGACCTCCAACCTGGGCTCGGCGCAGATTCAGGAACTGGTGGGTGACCGCGAGGCACAGCGTGCGGCa < 1:394523/136‑1 (MQ=255) gCTGGAAGACGGCCGCCTGACCGACAGCCACGGGCGCACCGTGGACTTCCGCAACACCGTGATCGTGATGACCTCCAACCTGGGCTCGGCGCAGATTCAGGAACTGGTGGGTGACCGCGAGGCACAGCGTGCGGCa < 1:112659/136‑1 (MQ=255) ggAAGACGGCCGCCTGACCGACAGCCACGGGCGCACCGTGGACTTCCGCAACACCGTGATCGTGATGACCTCCAACCTGGGCTCGGCGCAGATTCAGGAACTGGTGGGTGACCGCGAGGCACAGCGTGCGGCAGTg < 2:474384/136‑1 (MQ=255) ggAAGACGGCCGCCTGACCGACAGCCACGGGCGCACCGTGGACTTCCGCAACACCGTGATCGTGATGACCTCCAACCTGGGCTCGGCGCAGATTCAGGAACTGGTGGGTGACCGCGAGGCACAGCGTGCGGCAGTg < 1:153966/136‑1 (MQ=255) | TATGTAGGGTATGAAGAGGGCGGTTACCTGACCGAGGCCGTGCGGCGCAAGCCTTACTCGGTGGTGCTGCTGGACGAGGTGGAAAAAGCCCACCCGGATGTGTTCAACGTGCTGTTGCAGGTGCTGGAAGACGGCCGCCTGACCGACAGCCACGGGCGCACCGTGGACTTCCGCAACACCGTGATCGTGATGACCTCCAACCTGGGCTCGGCGCAGATTCAGGAACTGGTGGGTGACCGCGAGGCACAGCGTGCGGCAGTG > NC_002947/732420‑732680 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |