| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 922,569 | C→T | 15.4% | L154L (CTG→CTA) | PP_0803 ← | protein secretion ABC transporter permease |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 922,569 | 0 | C | T | 15.4% | 62.5 / 4.2 | 26 | L154L (CTG→CTA) | PP_0803 | protein secretion ABC transporter permease |
| Reads supporting (aligned to +/- strand): ref base C (8/14); new base T (2/2); total (10/16) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.25e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.09e-01 | |||||||||||
CGCTGGCGGTTCAGGCGGATCACTTCAACCCGGCTGGAGGCACCCATCTTTGCCAGGTTCTCGGTGATCTTCAGCTCGCTGCGCACCAGCTGCAACGAGTCCTGAATACCCGCCAGGGTCTGTTCCAGGCCGCGGCGGCGGGTCTGGTACAACGCGGTTTCGGCATCGATCAGGTCAGGAGAGTCGCGCAGGCTCTCCGGGAAGGTCAGTGGCTTGCCGGTCACCTCTGCCTGCAGGCGGGCCTGGCTGGCCCTGGCAGC > NC_002947/922444‑922703 | ctcTGGCGGTTCAGGCGGATCACTTCAACCCGGCTGGAGGCACCCATCTTTGCCAGGTTCTCGGTGATCTTCAGCTCGCTGCGCACCAGCTGCAACGAGTCCTGAATACCCGCCAGGGTCTGTTCCAGGCCgcggc < 1:345803/134‑1 (MQ=255)cGCTGGCGGTTCAGGCGGATCACTTCAACCCGGCTGGAGGCACCCATCTTTGCCAGGTTCTCGGTGATCTTCAGCTCGCTGCGCACCAGCTGCAACGAGTCCTGAATACCCGCCAGGGTCTGTTCCAGGCCgcggc < 2:59258/136‑1 (MQ=255)cGCTGGCGGTTCAGGCGGATCACTTCAACCCGGCTGGAGGCACCCATCTTTGCCAGGTTCTCGGTGATCTTCAGCTCGCTGCGCACCAGCTGCAACGAGTCCTGAATACCCGCCAGGGTCTGTTCCAGGCCgcggc > 2:305829/1‑136 (MQ=255)cGCTGGCGGTTCAGGCGGATCACTTCAACCCGGCTGGAGGCACCCATCTTTGCCAGGTTCTCGGTGATCTTCAGCTCGCTGCGCACCAGCTGCAACGAGTCCTGAATACCCGCCAGGGTCTGTTCCAGGCCgcggc > 2:173325/1‑136 (MQ=255)cGCTGGCGGTTCAGGCGGATCACTTCAACCCGGCTGGAGGCACCCATCTTTGCCAGGTTCTCGGTGATCTTCAGCTCGCTGCGCACCAGCTGCAACGAGTCCTGAATACCCGCCAGGGTCTGTTCCAGGCCgcggc < 1:165646/136‑1 (MQ=255) aTCACTTCAACCCGGCTGGAGGCACCCATCTTTGCCAGGTTCTCGGTGATCTTCAGCTCGCTGCGCACCAGCTGCAACGAGTCCTGAATACCCGCCAGGGTCTGTTCCAGGCCGCGGCGGCGGGTCTGGTACAAcg > 1:282798/1‑136 (MQ=255) aCTTCAACCCGGCTGGAGGCACCCATCTTTGCCAGGTTCTCGGTGATCTTCAGCTCGCTGCGCACCAGCTGCAACGAGTCCTGAATACCCGCCAGGGTCTGTTCCAGGCCGCGGCGGCGGGTCTGGTACAACGCgg < 1:472094/136‑1 (MQ=255) aaCCCGGCTGGAGGCACCCATCTTTGCCAGGTTCTCGGTGATCTTCAGCTCGCTGCGCACCAGCTGCAACGAGTCCTGAATACCCGCCAGGGTCTGTTCCAGGCCGCGGCGGCGGGTCTGGTACAACGCGGTTTCg < 1:306369/136‑1 (MQ=255) aaCCCGGCTGGAGGCACCCATCTTTGCCAGGTTCTCGGTGATCTTCAGCTCGCTGCGCACCAGCTGCAACGAGTCCTGAATACCCGCCAGGGTCTGTTCCAGGCCGCGGCGGCGGGTCTGGTACAACGCGGTTTCg < 1:253667/136‑1 (MQ=255) ggCTGGAGGCACCCATCTTTGCCAGGTTCTCGGTGATCTTCAGCTCGCTGCGCACCAGCTGCAACGAGTCCTGAATACCCGCCAGGGTCTGTTCCAGGCCGCGGCGGCGGGTCTGGTACAACGCGGTTTCGGCATc < 1:305829/136‑1 (MQ=255) ggCTGGAGGCACCCATCTTTGCCAGGTTCTCGGTGATCTTCAGCTCGCTGCGCACCAGCTGCAACGAGTCCTGAATACCCGCCAGGGTCTGTTCCAGGCCGCGGCGGCGGGTCTGGTACAACGCGGTTTCGGCATc > 1:426043/1‑136 (MQ=255) ggCTGGAGGCACCCATCTTTGCCAGGTTCTCGGTGATCTTCAGCTCGCTGCGCACCAGCTGCAACGAGTCCTGAATACCCGCCAGGGTCTGTTCCAGGCCGCGGCGGCGGGTCTGGTACAACGCGGTTTCGGCATc > 1:196667/1‑136 (MQ=255) ggCTGGAGGCACCCATCTTTGCCAGGTTCTCGGTGATCTTCAGCTCGCTGCGCACCAGCTGCAACGAGTCCTGAATACCCGCCAGGGTCTGTTCCAGGCCGCGGCGGCGGGTCTGGTACAACGCGGTTTCGGCATc < 1:173325/136‑1 (MQ=255) ggCTGGAGGCACCCATCTTTGCCAGGTTCTCGGTGATCTTCAGCTCGCTGCGCACCAGCTGCAACGAGTCCTGAATACCCACCAGGGTCTGTTCCAGGCCGCGGCGGCGGGTCTGGTACAACGCGGTTTCGGCATc < 2:392382/136‑1 (MQ=255) cAGGTTCTCGGTGATCTTCAGCTCGCTGCGCACCAGCTGCAACGAGTCCTGAATACCCGCCAGGGTCTGTTCCAGGCCGCGGCGGCGGGTCTGGTACAACGCGGTTTCGGCATCGATCAGGTCAGGAGAGTCgcgc < 2:282798/136‑1 (MQ=255) tcGGTGATCTTCAGCTCGCTGCGCACCAGCTGCAACGAGTCCTGAATACCCGCCAGGGTCTGTTCTAGGCCGCGGCGGCGGGTCTGGTACAACGCGGTTTCGGCATCGATCAGGTCAGGAGAGTCGCGCAGGctct > 2:416096/1‑136 (MQ=255) tcGGTGATCTTCAGCTCGCTGCGCACCAGCTGCAACGAGTCCTGAATACCCGCCAGGGTCTGTTCTAGGCCGCGGCGGCGGGTCTGGTACAACGCGGTTTCGGCATCGATCAGGTCAGGAGAGTCGCGCAGGctct > 2:386794/1‑136 (MQ=255) cGAGTCCTGAATACCCGCCAGGGTCTGTTCCAGGCCgcgg > 2:72945/1‑40 (MQ=255) cGAGTCCTGAATACCCGCCAGGGTCTGTTCCAGGCCgcgg < 1:72945/40‑1 (MQ=255) gTCCTGAATACCCGCCAGGGTCTGTTCCAGGCCGCGGCGGCGGGTCTGGTACAACGCGGTTTCGGCATCGATCAGGTCAGGAGAGTCGCGCAGGCTCTCCGGGAAGGTCAGTGGCTTGCCGGTCACCTctgcctgc < 2:426043/136‑1 (MQ=255) tGAATACCCGCCAGGGTCTGTTCCAGGCCGCGGCGGCGGGTCTGGTAc > 1:498930/1‑48 (MQ=255) tGAATACCCGCCAGGGTCTGTTCCAGGCCGCGGCGGCGGGTCTGGTAc < 2:498930/48‑1 (MQ=255) gAATACCCGCCAGGGTCTGTTCCAGGCCGCGGCGGCGGGTCTGGTACAACGCGGTTTCGGCATCGATCAGGTCAGGAGAGTCGCGCAGGCTCTCCGGGAAGGTCAGTGGCTTGCCGGTCACCTCTGCCTGCAGGCg < 2:287192/136‑1 (MQ=255) aCCCGCCAGGGTCTGTTCTAGGCCGCGGCGGCGGGTCTGGTACAACGCGGTTTCGGCATCGATCAGGTCAGGAGAGTCGCGCAGGCTCTCCGGGAAGGTCAGTGGCTTGCCGGTCACCTCTGCCTGCAGGCGGGcc < 1:416096/136‑1 (MQ=255) aCCCGCCAGGGTCTGTTCTAGGCCGCGGCGGCGGGTCTGGTACAACGCGGTTTCGGCATCGATCAGGTCAGGAGAGTCGCGCAGGCTCTCCGGGAAGGTCAGTGGCTTGCCGGTCACCTCTGCCTGCAGGCGGGcc < 1:386794/136‑1 (MQ=255) ccAGGGTCTGTTCCAGGCCGCGGCGGCGGGTCTGGTACAACGCGGTTTCGGCATCGATCAGGTCAGGAGAGTCGCGCAGGCTCTCCGGGAAGGTCAGTGGCTTGCCGGTCACCTCTGCCTGCAGGCGGGCctggct > 1:31927/1‑136 (MQ=255) ccAGGCCGCGGCGGCGGGTCTGGTACAACGCGGTTTCGGCATCGATCAGGTCAGGAGAGTCGCGCAGGCTCTCCGGGAAGGTCAGTGGCTTGCCGGTCACCTCTGCCTGCAGGCGGGCCTGGCTGGCCCTGGCAgc > 2:48926/1‑136 (MQ=255) | CGCTGGCGGTTCAGGCGGATCACTTCAACCCGGCTGGAGGCACCCATCTTTGCCAGGTTCTCGGTGATCTTCAGCTCGCTGCGCACCAGCTGCAACGAGTCCTGAATACCCGCCAGGGTCTGTTCCAGGCCGCGGCGGCGGGTCTGGTACAACGCGGTTTCGGCATCGATCAGGTCAGGAGAGTCGCGCAGGCTCTCCGGGAAGGTCAGTGGCTTGCCGGTCACCTCTGCCTGCAGGCGGGCCTGGCTGGCCCTGGCAGC > NC_002947/922444‑922703 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |