| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 1,486,664 | C→T | 19.2% | Y146Y (TAC→TAT) | yhdZ → | amino acid ABC transporter ATP‑binding protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 1,486,664 | 0 | C | T | 19.2% | 59.6 / 6.8 | 26 | Y146Y (TAC→TAT) | yhdZ | amino acid ABC transporter ATP‑binding protein |
| Reads supporting (aligned to +/- strand): ref base C (11/10); new base T (3/2); total (14/12) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.29e-01 | |||||||||||
CCCGCACCTGAGCATCCTGGAGAACTGCACCCTGGCGCCGATGTGGGTGCGCAAGATGCCACGGCGCAAGGCCGAGGAAATTGCCATGCACTACCTGGAGCGTGTGCGGATTCCGGAGCAGGCACACAAGTACCCTGGCCAGTTGTCTGGCGGCCAGCAGCAGCGTGTGGCCATTGCCCGCGCGTTGTGCATGAAGCCCAAGATCATGCTGTTCGACGAGCCGACCTCGGCACTGGACCCGGAAATGGTCAAGGAAGTGCTCG > NC_002947/1486532‑1486794 | cccGCACCTGAGCATCCTGGAGAACTGCACCCTGGCGCCGATGTGGGTGCGCAAGATGCCACGGCGCAAGGCCGAGGAAATTGCCATGCACTACCTGGAGCGTGTGCGGATTCCGGAGCAGGCACACAAGTACCCt > 1:253668/1‑136 (MQ=255) aCTGCACCCTGGCGCCGATGTGGGTGCGCAAGATGCCACGGCGCAAGGCCGAGGAAATTGCCATGCACTACCTGGAGCGTGTGCGGATTCCGGAGCAGGCACACAAGTACCCTGGCCAGTTGTCTGGCGGCcagca < 1:215121/136‑1 (MQ=255) ccGATTTGGGTACGCAAGATGCCAAAGAAGAAGGCGGAAGAAATCGCCATGCATTATCTGGCGCGCGTGAAAATTCCGGAACAGGCCAACAAATATCCGGGCCAGCTTTCAGGCGGCCAGCAGCAGCGTGTGGcga > 2:350972/1‑134 (MQ=255) ccGATTTGGGTACGCAAGATGCCAAAGAAGAAGGCGGAAGAAATCGCCATGCATTATCTGCCGCGCGTGAAAATTCCGGAACAGGCCAACAAATATCCGGGCCAGCTTTCAGGCGGCCAGCAGCAGCGTGTGGcga > 2:294619/1‑134 (MQ=255) gATGCCACGGCGCAAGGCCGAGGAAATTGCCATGCACTACCTGGAGCGTGTGCGGATTCCGGAGCAGGCACACAAGTACCCTGGCCAGTTGTCTGGCGGCCAGCAGCAGCGTGTGGCCATTGCCCGCGCGTTGTGc < 1:221703/136‑1 (MQ=255) gATGCCACGGCGCAAGGCCGAGGAAATTGCCATGCACTACCTGGAGCGTGTGCGGATTCCGGAGCAGGCACACAAGTACCCTGGCCAGTTGTCTGGCGGCCAGCAGCAGCGTGTGGCCATTGCCCGCGCGTTGTGc < 2:244470/136‑1 (MQ=255) aaGGCCGAGGAAATTGCCATGCACTACCTGGAGCGTGTGCGGATTCCGGAGCAGGCACACAAGTACCCTGGCCAGTTGTCTGGCGGCCAGCAGCAGCGTGTGGCCATTGCCCGCGCGTTGTGCATGAAGCCCAAGa < 2:253668/136‑1 (MQ=255) gAAATTGCCATGCACTACCTGGAGCGTGTGCGGATTCCGGAGCAGGCACACAAGTACCCTGGCCAGTTGTCTGGCGGCCAGCAGCAGCGTGTGGCCATTGCCCGCGCGTTGTGCATGAAGCCCAAGATCATGCTGt > 2:55615/1‑136 (MQ=255) aaaTTGCCATGCACTACCTGGAGCGTGTGCGGATTCCGGAGCAGGCACACAAGTACCCTGGCCAGTTGTCTGGCGGCCAGCAGCAGCGTGTGGCCATTGCCCGCGCGTTGTGCATGAAGCCCAAGATCATGCTGtt > 2:496324/1‑136 (MQ=255) aaaTTGCCATGCACTACCTGGAGCGTGTGCGGATTCCGGAGCAGGCACACAAGTACCCTGGCCAGTTGTCTGGCGGCCAGCAGCAGCGTGTGGCCATTGCCCGCGCGTTGTGCATGAAGCCCAAGATCATGCTGtt > 2:356319/1‑136 (MQ=255) gtgtGCGGATTCCGGAGCAGGCACACAAGTACCCTGGCCAGTTGTCTGGCGGCCAGCAGCAGCGTGTGGCCATTGCCCGCGCGTTGTGCATGAAGCCCAAGATCATGCTGTTCGACGAGCCGACCTCGGCACTGGa > 1:27457/1‑136 (MQ=255) tgCGGATTCCGGAGCAGGCACACAAGTACCCTGGCCAGTTGTCTGGCGGCCAGCAGCAGCGTGTGGCCATTGCCCGCGCGTTGTGCATGAAGCCCAAGATCATGCTGTTCGACGAGCCGACCTCGGCACTGGAccc > 1:231284/1‑136 (MQ=255) tgCGGATTCCGGAGCAGGCACACAAGTACCCTGGCCAGTTGTCTGGCGGCCAGCAGCAGCGTGTGGCCATTGCCCGCGCGTTGTGCATGAAGCCCAAGATCATGCTGTTCGACGAGCCGACCTCGGCACTGGAccc > 2:362665/1‑136 (MQ=255) gCGGATTCCGGAGCAGGCACACAAGTACCCTGGCCAGTTGTCTGGCGGCCAGCAGCAGCGTGTGGCCATTGCCCGCGCGTTGTGCATGAAGCCCAAGATCATGCTGTTCGACGAGCCGACCTCGGCACTGGACCCg < 1:356319/136‑1 (MQ=255) cGGATTCCGGAGCAGGCACACAAGTACCCTGGCCAGTTGTCTGGCGGCCAGCAGCAGCGTGTGGCCATTGCCCGCGCGTTGTGCATGAAGCCCAAGATCATGCTGTTCGACGAGCCGACCTCGGCACTGGACCCgg > 1:123704/1‑136 (MQ=255) cGGATTCCGGAGCAGGCACACAAGTACCCTGGCCAGTTGTCTGGCGGCCAGCAGCAGCGTGTGGCCATTGCCCGCGCGTTGTGCATGAAGCCCAAGATCATGCTGTTCGACGAGCCGACCTCGGCACTGGACCCgg > 1:138310/1‑136 (MQ=255) gtaTTCCCGATCAGGCCGATAAATATCCAAGCCAGTTGTCCGGCGGGCAGCAGCAGCGTGTCGCCATAGCGCGTTCCTTGTGCATGCAGCCCGACATCATCCTGTTCGACGAGCCGACCTCAGCCCTCGACCCTGa > 1:264948/3‑136 (MQ=12) ggATTCCGGAGCAGGCACACAAGTACCCTGGCCAGTTGTCTGGCGGCCAGCAGCAGCGTGTGGCCATTGCCCGTGCGTTGTGCATGAAGCCCAAGATCATGCTGTTCGACGAGCCGACCTCGGCACTGGACCCGGa > 2:486887/1‑136 (MQ=255) gaacAGGCCAACAAATATCCGGGCCAGCTTTCAGGCGGCCAGCAGCAGCGTGTGGCGATTGCCCGTGCGCTTTGCATGAACCCGAAGGTCATGCTGTTCGACGAGCCGACCTCGGCGCTCGATCCGGAAATGGTCa < 2:287990/133‑1 (MQ=9) caacAGGCCAACAAATATCCGGGCCAGCTTTCAGGCGGCCAGCAGCAGCGTGTGGCGATTGCCCGTGCGCTTTGCATGAACCCGAAGGTCATGCTGTTCGACGAGCCGACCTCGGCGCTCGATCCGGAAATGGTCa < 1:416217/133‑1 (MQ=9) cacacaAGTACCCTGGCCAGTTGTCTGGCGGCCAGCAGCAGCGTGTGGCCATTGCCCGCGCGTTGTGCATGAAGCCCAAGATCATGCTGTTCGACGAGCCGACCTCGGCACTGGACCCGGAAATGGTCAAGGAAGt < 1:496324/136‑1 (MQ=255) cacacaAGTACCCTGGCCAGTTGTCTGGCGGCCAGCAGCAGCGTGTGGCCATTGCCCGCGCGTTGTGCATGAAGCCCAAGATCATGCTGTTCGACGAGCCGACCTCGGCACTGGACCCGGAAATGGTCAAGGAAGt < 1:263422/136‑1 (MQ=255) cacacaAGTACCCTGGCCAGTTGTCTGGCGGCCAGCAGCAGCGTGTGGCCATTGCCCGCGCGTTGTGCATGAAGCCCAAGATCATGCTGTTCGACGAGCCGACCTCGGCACTGGACCCGGAAATGGTCAAGGAAGt < 1:262922/136‑1 (MQ=255) cacaAGTACCCTGGCCAGTTGTCTGGCGGCCAGCAGCAGCGTGTGGCCATTGCCCGCGCGTTGTGCATGAAGCCCAAGATCATGCTGTTCGACGAGCCGACCTCGGCACTGGACCCGGAAATGGTCAAg < 2:1012/129‑1 (MQ=255) cacaAGTACCCTGGCCAGTTGTCTGGCGGCCAGCAGCAGCGTGTGGCCATTGCCCGCGCGTTGTGCATGAAGCCCAAGATCATGCTGTTCGACGAGCCGACCTCGGCACTGGACCCGGAAATGGTCAAg > 1:1012/1‑129 (MQ=255) aaGTACCCTGGCCAGTTGTCTGGCGGCCAGCAGCAGCGTGTGGCCATTGCCCGCGCGTTGTGCATGAAGCCCAAGATCATGCTGTTCGACGAGCCGACCTCGGCACTGGACCCGGAAATGGTCAAGGAAGTGCTCg < 1:55615/136‑1 (MQ=255) | CCCGCACCTGAGCATCCTGGAGAACTGCACCCTGGCGCCGATGTGGGTGCGCAAGATGCCACGGCGCAAGGCCGAGGAAATTGCCATGCACTACCTGGAGCGTGTGCGGATTCCGGAGCAGGCACACAAGTACCCTGGCCAGTTGTCTGGCGGCCAGCAGCAGCGTGTGGCCATTGCCCGCGCGTTGTGCATGAAGCCCAAGATCATGCTGTTCGACGAGCCGACCTCGGCACTGGACCCGGAAATGGTCAAGGAAGTGCTCG > NC_002947/1486532‑1486794 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |