Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 1,486,712 C→T 20.5% R162R (CGC→CGT)  yhdZ → amino acid ABC transporter ATP‑binding protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029471,486,7120CT20.5% 79.1 / 11.4 34R162R (CGC→CGT) yhdZamino acid ABC transporter ATP‑binding protein
Reads supporting (aligned to +/- strand):  ref base C (13/14);  new base T (2/5);  total (15/19)
Fisher's exact test for biased strand distribution p-value = 4.26e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.60e-01

GATGCCACGGCGCAAGGCCGAGGAAATTGCCATGCACTACCTGGAGCGTGTGCGGATTCCGGAGCAGGCACACAAGTACCCTGGCCAGTTGTCTGGCGGCCAGCAGCAGCGTGTGGCCATTGCCCGCGCGTTGTGCATGAAGCCCAAGATCATGCTGTTCGACGAGCCGACCTCGGCACTGGACCCGGAAATGGTCAAGGAAGTGCTCGATACCATGGTGGGCCTGGCCGAGGATGGCATGACCATGCTCTGCGTGAC  >  NC_002947/1486586‑1486843
                                                                                                                              |                                                                                                                                   
gATGCCACGGCGCAAGGCCGAGGAAATTGCCATGCACTACCTGGAGCGTGTGCGGATTCCGGAGCAGGCACACAAGTACCCTGGCCAGTTGTCTGGCGGCCAGCAGCAGCGTGTGGCCATTGCCCGCGCGTTGTGc                                                                                                                            <  2:244470/136‑1 (MQ=255)
gATGCCACGGCGCAAGGCCGAGGAAATTGCCATGCACTACCTGGAGCGTGTGCGGATTCCGGAGCAGGCACACAAGTACCCTGGCCAGTTGTCTGGCGGCCAGCAGCAGCGTGTGGCCATTGCCCGCGCGTTGTGc                                                                                                                            <  1:221703/136‑1 (MQ=255)
             aaGGCCGAGGAAATTGCCATGCACTACCTGGAGCGTGTGCGGATTCCGGAGCAGGCACACAAGTACCCTGGCCAGTTGTCTGGCGGCCAGCAGCAGCGTGTGGCCATTGCCCGCGCGTTGTGCATGAAGCCCAAGa                                                                                                               <  2:253668/136‑1 (MQ=255)
                      gAAATTGCCATGCACTACCTGGAGCGTGTGCGGATTCCGGAGCAGGCACACAAGTACCCTGGCCAGTTGTCTGGCGGCCAGCAGCAGCGTGTGGCCATTGCCCGCGCGTTGTGCATGAAGCCCAAGATCATGCTGt                                                                                                      >  2:55615/1‑136 (MQ=255)
                       aaaTTGCCATGCACTACCTGGAGCGTGTGCGGATTCCGGAGCAGGCACACAAGTACCCTGGCCAGTTGTCTGGCGGCCAGCAGCAGCGTGTGGCCATTGCCCGCGCGTTGTGCATGAAGCCCAAGATCATGCTGtt                                                                                                     >  2:496324/1‑136 (MQ=255)
                       aaaTTGCCATGCACTACCTGGAGCGTGTGCGGATTCCGGAGCAGGCACACAAGTACCCTGGCCAGTTGTCTGGCGGCCAGCAGCAGCGTGTGGCCATTGCCCGCGCGTTGTGCATGAAGCCCAAGATCATGCTGtt                                                                                                     >  2:356319/1‑136 (MQ=255)
                                               gtgtGCGGATTCCGGAGCAGGCACACAAGTACCCTGGCCAGTTGTCTGGCGGCCAGCAGCAGCGTGTGGCCATTGCCCGCGCGTTGTGCATGAAGCCCAAGATCATGCTGTTCGACGAGCCGACCTCGGCACTGGa                                                                             >  1:27457/1‑136 (MQ=255)
                                                  tgCGGATTCCGGAGCAGGCACACAAGTACCCTGGCCAGTTGTCTGGCGGCCAGCAGCAGCGTGTGGCCATTGCCCGCGCGTTGTGCATGAAGCCCAAGATCATGCTGTTCGACGAGCCGACCTCGGCACTGGAccc                                                                          >  1:231284/1‑136 (MQ=255)
                                                  tgCGGATTCCGGAGCAGGCACACAAGTACCCTGGCCAGTTGTCTGGCGGCCAGCAGCAGCGTGTGGCCATTGCCCGCGCGTTGTGCATGAAGCCCAAGATCATGCTGTTCGACGAGCCGACCTCGGCACTGGAccc                                                                          >  2:362665/1‑136 (MQ=255)
                                                   gCGGATTCCGGAGCAGGCACACAAGTACCCTGGCCAGTTGTCTGGCGGCCAGCAGCAGCGTGTGGCCATTGCCCGCGCGTTGTGCATGAAGCCCAAGATCATGCTGTTCGACGAGCCGACCTCGGCACTGGACCCg                                                                         <  1:356319/136‑1 (MQ=255)
                                                    cGGATTCCGGAGCAGGCACACAAGTACCCTGGCCAGTTGTCTGGCGGCCAGCAGCAGCGTGTGGCCATTGCCCGCGCGTTGTGCATGAAGCCCAAGATCATGCTGTTCGACGAGCCGACCTCGGCACTGGACCCgg                                                                        >  1:138310/1‑136 (MQ=255)
                                                    cGGATTCCGGAGCAGGCACACAAGTACCCTGGCCAGTTGTCTGGCGGCCAGCAGCAGCGTGTGGCCATTGCCCGCGCGTTGTGCATGAAGCCCAAGATCATGCTGTTCGACGAGCCGACCTCGGCACTGGACCCgg                                                                        >  1:123704/1‑136 (MQ=255)
                                                     gtaTTCCCGATCAGGCCGATAAATATCCAAGCCAGTTGTCCGGCGGGCAGCAGCAGCGTGTCGCCATAGCGCGTTCCTTGTGCATGCAGCCCGACATCATCCTGTTCGACGAGCCGACCTCAGCCCTCGACCCTGa                                                                       >  1:264948/3‑136 (MQ=12)
                                                     ggATTCCGGAGCAGGCACACAAGTACCCTGGCCAGTTGTCTGGCGGCCAGCAGCAGCGTGTGGCCATTGCCCGTGCGTTGTGCATGAAGCCCAAGATCATGCTGTTCGACGAGCCGACCTCGGCACTGGACCCGGa                                                                       >  2:486887/1‑136 (MQ=255)
                                                             gaacAGGCCAACAAATATCCGGGCCAGCTTTCAGGCGGCCAGCAGCAGCGTGTGGCGATTGCCCGTGCGCTTTGCATGAACCCGAAGGTCATGCTGTTCGACGAGCCGACCTCGGCGCTCGATCCGGAAATGGTCa                                                               <  2:287990/133‑1 (MQ=9)
                                                             caacAGGCCAACAAATATCCGGGCCAGCTTTCAGGCGGCCAGCAGCAGCGTGTGGCGATTGCCCGTGCGCTTTGCATGAACCCGAAGGTCATGCTGTTCGACGAGCCGACCTCGGCGCTCGATCCGGAAATGGTCa                                                               <  1:416217/133‑1 (MQ=9)
                                                                    cacacaAGTACCCTGGCCAGTTGTCTGGCGGCCAGCAGCAGCGTGTGGCCATTGCCCGCGCGTTGTGCATGAAGCCCAAGATCATGCTGTTCGACGAGCCGACCTCGGCACTGGACCCGGAAATGGTCAAGGAAGt                                                        <  1:496324/136‑1 (MQ=255)
                                                                    cacacaAGTACCCTGGCCAGTTGTCTGGCGGCCAGCAGCAGCGTGTGGCCATTGCCCGCGCGTTGTGCATGAAGCCCAAGATCATGCTGTTCGACGAGCCGACCTCGGCACTGGACCCGGAAATGGTCAAGGAAGt                                                        <  1:263422/136‑1 (MQ=255)
                                                                    cacacaAGTACCCTGGCCAGTTGTCTGGCGGCCAGCAGCAGCGTGTGGCCATTGCCCGCGCGTTGTGCATGAAGCCCAAGATCATGCTGTTCGACGAGCCGACCTCGGCACTGGACCCGGAAATGGTCAAGGAAGt                                                        <  1:262922/136‑1 (MQ=255)
                                                                      cacaAGTACCCTGGCCAGTTGTCTGGCGGCCAGCAGCAGCGTGTGGCCATTGCCCGCGCGTTGTGCATGAAGCCCAAGATCATGCTGTTCGACGAGCCGACCTCGGCACTGGACCCGGAAATGGTCAAg                                                             >  1:1012/1‑129 (MQ=255)
                                                                      cacaAGTACCCTGGCCAGTTGTCTGGCGGCCAGCAGCAGCGTGTGGCCATTGCCCGCGCGTTGTGCATGAAGCCCAAGATCATGCTGTTCGACGAGCCGACCTCGGCACTGGACCCGGAAATGGTCAAg                                                             <  2:1012/129‑1 (MQ=255)
                                                                         aaGTACCCTGGCCAGTTGTCTGGCGGCCAGCAGCAGCGTGTGGCCATTGCCCGCGCGTTGTGCATGAAGCCCAAGATCATGCTGTTCGACGAGCCGACCTCGGCACTGGACCCGGAAATGGTCAAGGAAGTGCTCg                                                   <  1:55615/136‑1 (MQ=255)
                                                                                cTGGCCAGTTGTCTGGCGGCCAGCAGCAGCGTGTGGCCATTGCCCGCGCGTTGTGCATGAAGCCCAAGATCATGCTGTTCGACGAGCCGACCTCGGCACTGGACCCGGAAATGGTCAAGGAAGTGCTCGATACCAt                                            <  2:435037/136‑1 (MQ=255)
                                                                                  agCCAGTTGTCCGGCGGGCAGCAGCAGCGTGTCGCCATAGCGCGTTCCTTGTGCATGCAGCCCGACATCATCCTGTTCGACGAGCCGACCTCAGCCCTCGACCCTGAACTGGTGCGCGAAGTTCTCGATACGAtgg                                          <  2:399601/135‑1 (MQ=12)
                                                                                    ccAGTTGTCTGGCGGCCAGCAGCAGCGTGTGGCCATTGCCCGCGCGTTGTGCATGAAGCCCAAGATCATGCTGTTCGACGAGCCGACCTCGGCACTGGACCCGGAAATGGTCAAGGAAGTGCTCGATACCAtggtg                                        >  1:120438/1‑136 (MQ=255)
                                                                                          gTCCGGCGGGCAGCAGAAGCGTGTCGCCATAGCGCGTGCCTGGTGCATGCAGCCCGACATCATCCTGTTCGACGAGCCGACCTCAGGCCTCGACCCTGAACTGGTGCGCGAAGTTCTCGATACGAtggtgtctctc                                  <  2:29903/136‑7 (MQ=255)
                                                                                             aggcggcCAGCAGCAGCGTGGGGCGATTGCCCGTGCGCTTTGCATGAACCCGAAGGTCATGCTGTTCGACGAGCCGACCTCGGCGCTCGATCCGGAAATGGTCAAGGAAGTGCTGGATACGATGGTGAGTCTTGcc                               <  2:198124/135‑1 (MQ=17)
                                                                                                    cagcagcagCGTGTGGCCATTGCCCGCGCGTTGTGCATGAAGCCCAAGATCATGCTGTTCGACGAGCCGACCTCGGCACTGGACCCGGAAATGGTCAAGGAAGTGCTCGATACCATGGTGGGCCTGGCCGAGGATg                        <  2:250628/136‑1 (MQ=255)
                                                                                                    cagcagcagCGTGTGGCCATTGCCCGCGCGTTGTGCATGAAGCCCAAGATCATGCTGTTCGACGAGCCGACCTCGGCACTGGACCCGGAAATGGTCAAGGAAGTGCTCGATACCATGGTGGGCCTGGCCGAGGATg                        <  1:267491/136‑1 (MQ=255)
                                                                                                     agcagcagcGTGTGGCCATTGCCCGCGCGTTGTGCATGAAGCCCAAGATCATGCTGTTCGACGAGCCGACCTCGGCACTGGACCCGGAAATGGTCAAGGAAGTGCTCGATACCATGGTGGGCCTGGCCGAGGATgg                       >  1:452903/1‑136 (MQ=255)
                                                                                                                      aTTGCCCGCGCGTTGTGCATGAAGCCCAAGATCATGCGGTTCGACGAGCCGACCTCGGCACTGGACCCGGAAATGGTCAAGGAAGTGCTCGATACCATGGGGGGCCTGGCCGAGGATGGCATGACCATGCTCTGCg      <  2:345550/136‑1 (MQ=255)
                                                                                                                        tGCCCGCGCGTTGTGCATGAAGCCGAAGATCATGCTGTTCGACGAGCAGACCTCGGCACTGGTCCGGGTAATGTTCTAGGAAGTGCTAGATACCATGGTGTGCCTGGCCGAGGATGGCATGACCATGCTCTGCGTg    <  2:178066/136‑1 (MQ=38)
                                                                                                                          cccGCGCGTTGTGCATGAAGCCCAAGATCATGCTGTTCGACGAGCCGACCTCGGCACTGGACCCGGAAATGGTCAAGGAAGTGCTCGATACCATGGTGGGCCTGGCCGAGGATGGCATGACCATGCTCTGCGTGAc  >  1:437068/1‑136 (MQ=255)
                                                                                                                          cccGCGCGTTGTGCATGAAGCCCAAGATCATGCTGTTCGACGAGCCGACCTCGGCACTGGACCCGGAAATGGTCAAGGAAGTGCTCGATACCATGGTGGGCCTGGCCGACGATGGCATGACCATGCTCTGCGTGAc  >  1:205139/1‑136 (MQ=255)
                                                                                                                              |                                                                                                                                   
GATGCCACGGCGCAAGGCCGAGGAAATTGCCATGCACTACCTGGAGCGTGTGCGGATTCCGGAGCAGGCACACAAGTACCCTGGCCAGTTGTCTGGCGGCCAGCAGCAGCGTGTGGCCATTGCCCGCGCGTTGTGCATGAAGCCCAAGATCATGCTGTTCGACGAGCCGACCTCGGCACTGGACCCGGAAATGGTCAAGGAAGTGCTCGATACCATGGTGGGCCTGGCCGAGGATGGCATGACCATGCTCTGCGTGAC  >  NC_002947/1486586‑1486843

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑