| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 1,486,766 | G→C | 21.4% | L180L (CTG→CTC) | yhdZ → | amino acid ABC transporter ATP‑binding protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 1,486,766 | 0 | G | C | 21.4% | 98.8 / 17.0 | 37 | L180L (CTG→CTC) | yhdZ | amino acid ABC transporter ATP‑binding protein |
| Reads supporting (aligned to +/- strand): ref base G (14/15); new base C (3/5); total (17/20) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 7.01e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
GTGTGCGGATTCCGGAGCAGGCACACAAGTACCCTGGCCAGTTGTCTGGCGGCCAGCAGCAGCGTGTGGCCATTGCCCGCGCGTTGTGCATGAAGCCCAAGATCATGCTGTTCGACGAGCCGACCTCGGCACTGGACCCGGAAATGGTCAAGGAAGTGCTCGATACCATGGTGGGCCTGGCCGAGGATGGCATGACCATGCTCTGCGTGACCCACGAAATGGGCTTTGCCCGTACCGTGGCGAACCGGGTTATCTTCATG > NC_002947/1486633‑1486892 | gtgtGCGGATTCCGGAGCAGGCACACAAGTACCCTGGCCAGTTGTCTGGCGGCCAGCAGCAGCGTGTGGCCATTGCCCGCGCGTTGTGCATGAAGCCCAAGATCATGCTGTTCGACGAGCCGACCTCGGCACTGGa > 1:27457/1‑136 (MQ=255) tgCGGATTCCGGAGCAGGCACACAAGTACCCTGGCCAGTTGTCTGGCGGCCAGCAGCAGCGTGTGGCCATTGCCCGCGCGTTGTGCATGAAGCCCAAGATCATGCTGTTCGACGAGCCGACCTCGGCACTGGAccc > 2:362665/1‑136 (MQ=255) tgCGGATTCCGGAGCAGGCACACAAGTACCCTGGCCAGTTGTCTGGCGGCCAGCAGCAGCGTGTGGCCATTGCCCGCGCGTTGTGCATGAAGCCCAAGATCATGCTGTTCGACGAGCCGACCTCGGCACTGGAccc > 1:231284/1‑136 (MQ=255) gCGGATTCCGGAGCAGGCACACAAGTACCCTGGCCAGTTGTCTGGCGGCCAGCAGCAGCGTGTGGCCATTGCCCGCGCGTTGTGCATGAAGCCCAAGATCATGCTGTTCGACGAGCCGACCTCGGCACTGGACCCg < 1:356319/136‑1 (MQ=255) cGGATTCCGGAGCAGGCACACAAGTACCCTGGCCAGTTGTCTGGCGGCCAGCAGCAGCGTGTGGCCATTGCCCGCGCGTTGTGCATGAAGCCCAAGATCATGCTGTTCGACGAGCCGACCTCGGCACTGGACCCgg > 1:123704/1‑136 (MQ=255) cGGATTCCGGAGCAGGCACACAAGTACCCTGGCCAGTTGTCTGGCGGCCAGCAGCAGCGTGTGGCCATTGCCCGCGCGTTGTGCATGAAGCCCAAGATCATGCTGTTCGACGAGCCGACCTCGGCACTGGACCCgg > 1:138310/1‑136 (MQ=255) gtaTTCCCGATCAGGCCGATAAATATCCAAGCCAGTTGTCCGGCGGGCAGCAGCAGCGTGTCGCCATAGCGCGTTCCTTGTGCATGCAGCCCGACATCATCCTGTTCGACGAGCCGACCTCAGCCCTCGACCCTGa > 1:264948/3‑136 (MQ=12) ggATTCCGGAGCAGGCACACAAGTACCCTGGCCAGTTGTCTGGCGGCCAGCAGCAGCGTGTGGCCATTGCCCGTGCGTTGTGCATGAAGCCCAAGATCATGCTGTTCGACGAGCCGACCTCGGCACTGGACCCGGa > 2:486887/1‑136 (MQ=255) gaacAGGCCAACAAATATCCGGGCCAGCTTTCAGGCGGCCAGCAGCAGCGTGTGGCGATTGCCCGTGCGCTTTGCATGAACCCGAAGGTCATGCTGTTCGACGAGCCGACCTCGGCGCTCGATCCGGAAATGGTCa < 2:287990/133‑1 (MQ=9) caacAGGCCAACAAATATCCGGGCCAGCTTTCAGGCGGCCAGCAGCAGCGTGTGGCGATTGCCCGTGCGCTTTGCATGAACCCGAAGGTCATGCTGTTCGACGAGCCGACCTCGGCGCTCGATCCGGAAATGGTCa < 1:416217/133‑1 (MQ=9) cacacaAGTACCCTGGCCAGTTGTCTGGCGGCCAGCAGCAGCGTGTGGCCATTGCCCGCGCGTTGTGCATGAAGCCCAAGATCATGCTGTTCGACGAGCCGACCTCGGCACTGGACCCGGAAATGGTCAAGGAAGt < 1:496324/136‑1 (MQ=255) cacacaAGTACCCTGGCCAGTTGTCTGGCGGCCAGCAGCAGCGTGTGGCCATTGCCCGCGCGTTGTGCATGAAGCCCAAGATCATGCTGTTCGACGAGCCGACCTCGGCACTGGACCCGGAAATGGTCAAGGAAGt < 1:263422/136‑1 (MQ=255) cacacaAGTACCCTGGCCAGTTGTCTGGCGGCCAGCAGCAGCGTGTGGCCATTGCCCGCGCGTTGTGCATGAAGCCCAAGATCATGCTGTTCGACGAGCCGACCTCGGCACTGGACCCGGAAATGGTCAAGGAAGt < 1:262922/136‑1 (MQ=255) cacaAGTACCCTGGCCAGTTGTCTGGCGGCCAGCAGCAGCGTGTGGCCATTGCCCGCGCGTTGTGCATGAAGCCCAAGATCATGCTGTTCGACGAGCCGACCTCGGCACTGGACCCGGAAATGGTCAAg > 1:1012/1‑129 (MQ=255) cacaAGTACCCTGGCCAGTTGTCTGGCGGCCAGCAGCAGCGTGTGGCCATTGCCCGCGCGTTGTGCATGAAGCCCAAGATCATGCTGTTCGACGAGCCGACCTCGGCACTGGACCCGGAAATGGTCAAg < 2:1012/129‑1 (MQ=255) aaGTACCCTGGCCAGTTGTCTGGCGGCCAGCAGCAGCGTGTGGCCATTGCCCGCGCGTTGTGCATGAAGCCCAAGATCATGCTGTTCGACGAGCCGACCTCGGCACTGGACCCGGAAATGGTCAAGGAAGTGCTCg < 1:55615/136‑1 (MQ=255) cTGGCCAGTTGTCTGGCGGCCAGCAGCAGCGTGTGGCCATTGCCCGCGCGTTGTGCATGAAGCCCAAGATCATGCTGTTCGACGAGCCGACCTCGGCACTGGACCCGGAAATGGTCAAGGAAGTGCTCGATACCAt < 2:435037/136‑1 (MQ=255) agCCAGTTGTCCGGCGGGCAGCAGCAGCGTGTCGCCATAGCGCGTTCCTTGTGCATGCAGCCCGACATCATCCTGTTCGACGAGCCGACCTCAGCCCTCGACCCTGAACTGGTGCGCGAAGTTCTCGATACGAtgg < 2:399601/135‑1 (MQ=12) ccAGTTGTCTGGCGGCCAGCAGCAGCGTGTGGCCATTGCCCGCGCGTTGTGCATGAAGCCCAAGATCATGCTGTTCGACGAGCCGACCTCGGCACTGGACCCGGAAATGGTCAAGGAAGTGCTCGATACCAtggtg > 1:120438/1‑136 (MQ=255) gTCCGGCGGGCAGCAGAAGCGTGTCGCCATAGCGCGTGCCTGGTGCATGCAGCCCGACATCATCCTGTTCGACGAGCCGACCTCAGGCCTCGACCCTGAACTGGTGCGCGAAGTTCTCGATACGAtggtgtctctc < 2:29903/136‑7 (MQ=255) aggcggcCAGCAGCAGCGTGGGGCGATTGCCCGTGCGCTTTGCATGAACCCGAAGGTCATGCTGTTCGACGAGCCGACCTCGGCGCTCGATCCGGAAATGGTCAAGGAAGTGCTGGATACGATGGTGAGTCTTGcc < 2:198124/135‑1 (MQ=17) cagcagcagCGTGTGGCCATTGCCCGCGCGTTGTGCATGAAGCCCAAGATCATGCTGTTCGACGAGCCGACCTCGGCACTGGACCCGGAAATGGTCAAGGAAGTGCTCGATACCATGGTGGGCCTGGCCGAGGATg < 1:267491/136‑1 (MQ=255) cagcagcagCGTGTGGCCATTGCCCGCGCGTTGTGCATGAAGCCCAAGATCATGCTGTTCGACGAGCCGACCTCGGCACTGGACCCGGAAATGGTCAAGGAAGTGCTCGATACCATGGTGGGCCTGGCCGAGGATg < 2:250628/136‑1 (MQ=255) agcagcagcGTGTGGCCATTGCCCGCGCGTTGTGCATGAAGCCCAAGATCATGCTGTTCGACGAGCCGACCTCGGCACTGGACCCGGAAATGGTCAAGGAAGTGCTCGATACCATGGTGGGCCTGGCCGAGGATgg > 1:452903/1‑136 (MQ=255) aTTGCCCGCGCGTTGTGCATGAAGCCCAAGATCATGCGGTTCGACGAGCCGACCTCGGCACTGGACCCGGAAATGGTCAAGGAAGTGCTCGATACCATGGGGGGCCTGGCCGAGGATGGCATGACCATGCTCTGCg < 2:345550/136‑1 (MQ=255) tGCCCGCGCGTTGTGCATGAAGCCGAAGATCATGCTGTTCGACGAGCAGACCTCGGCACTGGTCCGGGTAATGTTCTAGGAAGTGCTAGATACCATGGTGTGCCTGGCCGAGGATGGCATGACCATGCTCTGCGTg < 2:178066/136‑1 (MQ=38) cccGCGCGTTGTGCATGAAGCCCAAGATCATGCTGTTCGACGAGCCGACCTCGGCACTGGACCCGGAAATGGTCAAGGAAGTGCTCGATACCATGGTGGGCCTGGCCGAGGATGGCATGACCATGCTCTGCGTGAc > 1:437068/1‑136 (MQ=255) cccGCGCGTTGTGCATGAAGCCCAAGATCATGCTGTTCGACGAGCCGACCTCGGCACTGGACCCGGAAATGGTCAAGGAAGTGCTCGATACCATGGTGGGCCTGGCCGACGATGGCATGACCATGCTCTGCGTGAc > 1:205139/1‑136 (MQ=255) gctttgCATGAACCCGAAGGTCATGCTGTTCGACGAGCCGACCTCGGCGCTCGATCCGGAAATGGTCAAGGAAGTGCTGGATACGATGGTGAGTCTTGCCGATGAAGGCATGACCATGATCTGTGTGACGCACGat > 1:352492/5‑135 (MQ=12) gtgCATGAAGNCCAAGATCATGCTGTTCGACGAGCCGACCTCGGNACTGGANCCGGAAATGGTCAAGGAAGTGCTCGATACCATGGTGGGCCTGGCCGAGGATGGCATGACCNTGCTCTGCGTGACCCACGAAATg < 2:171157/136‑1 (MQ=255) gtgCATGAAGCCCAAGATCATGCTGTTCGACGAGCCGACCTCGGCACTGGACCCGGAAATGGTCAAGGAAGTGCTCGATACCATGGTGGGCCTGGCCGAGGATGGCATGACCATGCTCTGCGTGACCCACGAAATg < 2:452903/136‑1 (MQ=255) aTGAAGCCCAAGATCATGCTGTTCGACGAGCCGACCTCGGCACTGGACCCGGAAATGGTCAAGGAAGTGCTCGATACCATGGTGGGCCTGGCCGAGGATGGCATGACCATGCTCTGCGTGACCCACGAAATGGGCt > 2:118542/1‑136 (MQ=255) aGATCATGCTGTTCGACGAGCCGACCTCGGCACTGGACCCGGAAATGGTCAAGGAAGTGCTCGATACCATGGTGGGCCTGGCCGAGGATGGCATGACCATGCTCTGCGTGACCCACGAAATGGGCTTTGCCCGTAc > 2:26675/1‑136 (MQ=255) gtCATGCTGTTCGACGAGCCGACCTCGGCGCTCGATCCGGAAATGGTCAAGGAAGTGCTGGATACGATGGTGAGTCTTGCCGATGAAGGCATGACCATGATCTGTGTGACGCACGAAATGGGCTTTGCGCGtcagg > 2:466503/2‑132 (MQ=17) tGTTCGACGAGCCGACCTCGGCACTGGACCCGGAAATGGTCAAGGAAGTGCTCGATACCATGGTGGGCCTGGCCGAGGATGGCATGACCATGCTCTGCGTGACCCACGAAATGGGCTTTGCCCGTACCGTGGCGaa > 1:483496/1‑136 (MQ=255) cTCGGCACTGGACCCGGAAATGGTCAAGGAAGTGCTCGATACCATGGTGGGCCTGGCCGAGGATGGCATGACCATGCTCTGCGTGACCCACGAAATGGGCTTTGCCCGTACCGTGGCGAACCGGGTTATCTTCATg < 1:145190/136‑1 (MQ=255) cTCGGCACTGGACCCGGAAATGGTCAAGGAAGTGCTCGATACCATGGTGGGCCTGGCCGAGGATGGCATGACCATGCTCTGCGTGACCCACGAAATGGGCTTTGCCCGTACCGTGGCGAACCGGGTTATCTTCATg < 2:483496/136‑1 (MQ=255) | GTGTGCGGATTCCGGAGCAGGCACACAAGTACCCTGGCCAGTTGTCTGGCGGCCAGCAGCAGCGTGTGGCCATTGCCCGCGCGTTGTGCATGAAGCCCAAGATCATGCTGTTCGACGAGCCGACCTCGGCACTGGACCCGGAAATGGTCAAGGAAGTGCTCGATACCATGGTGGGCCTGGCCGAGGATGGCATGACCATGCTCTGCGTGACCCACGAAATGGGCTTTGCCCGTACCGTGGCGAACCGGGTTATCTTCATG > NC_002947/1486633‑1486892 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |