| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 1,800,621 | C→T | 18.2% | A188V (GCC→GTC) | dnaEA → | DNA polymerase III subunit alpha |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 1,800,621 | 0 | C | T | 18.2% | 46.1 / 4.6 | 22 | A188V (GCC→GTC) | dnaEA | DNA polymerase III subunit alpha |
| Reads supporting (aligned to +/- strand): ref base C (7/11); new base T (2/2); total (9/13) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.80e-01 | |||||||||||
GCGGCAAGACGAAGCCGAAGCGCTTCTTCAAGACTGGATGGGCATGTTCCCCGAGCGCTTCTACGTTGAAGTGCAGCGCACCAATCGGGCACGCGACGAAGAATACGTGCACGCGGCGGTGGCATTGGCCGACAAGCTTGGCGCCCCGCTGGTGGCTACCAACGACGTACGTTTCATCAAGCAGGCGGACTTCGACGCCCACGAGACCCGCGTATGCATTGGCGAGGGATGGACCCTTGACGACCCGCGCCGT > NC_002947/1800493‑1800745 | gcggcAAGACGAAGCCGAAGCGCTTCTTCAAGACTGGATGGGCATGTTCCCCGAGCGCTTCTACGTTGAAGTGCAGCGCACCAATCGGGCACGCGACGAAGAATACGTGCACGCGGCGGTGGCATTGGCCGACAAg > 2:296752/1‑136 (MQ=255) gcAAGACGAAGCCGAAGCGCTTCTTCAAGACTGGATGGGCATGTTCCCCGAGCGCTTCTACGTTGAAGTGCAGCGCACCAATCGGGCACGCGACGAAGAATACGTGCACGCGGCGGTGGCATTGGTCGACAAGCtt > 2:377751/1‑136 (MQ=255) gcAAGACGAAGCCGAAGCGCTTCTTCAAGACTGGATGGGCATGTTCCCCGAGCGCTTCTACGTTGAAGTGCAGCGCACCAATCGGGCACGCGACGAAGAATACGTGCACGCGGCGGTGGCATTGGTCGACAAGCtt > 2:100469/1‑136 (MQ=255) aaGACGAAGCCGAAGCGCTTCTTCAAGACTGGATGGGCATGTTCCCCGAGCGCTTCTACGTTGAAGTGCAGCGCACCAATCGGGCACGCGACGAAGAATACGTGCACGCGGCGGTGGCATTGGCCGACAAGCTTgg < 1:296752/136‑1 (MQ=255) agccgaagcGCTTCTTCAAGACTGGATGGGCATGTTCCCCGAGCGCTTCTACGTTGAAGTGCAGCGCACCAATCGGGCACGCGACGAAGAATACGTGCACGCGGCGGTGGCATTGGCCGACAAGCTTGGCGCCCCg < 1:44057/136‑1 (MQ=255) tGGGCATGTTCCCCGAGCGCTTCTACGTTGAAGTGCAGCGCACCAATCGGGCACGCGACGAAGAATACGTGCACGCGGCGGTGGCATTGGCCGACAAGCTTGGCGCCCCGCTGGTGGCTACCAACGACGTACGttt < 2:469674/136‑1 (MQ=255) gggCATGTTCCCCGAGCGCTTCTACGTTGAAGTGCAGCGCACCAATCGGGCACGCGACGAAGAATACGTGCACGCGGCGGTGGCATTGGCCGACAAGCTTGGCGCCCCGCTGGTGGCTACCAACGACGTACGTTtc > 1:432634/1‑136 (MQ=255) gggCATGTTCCCCGAGCGCTTCTACGTTGAAGTGCAGCGCACCAATCGGGCACGCGACGAAGAATACGTGCACGCGGCGGTGGCATTGGCCGACAAGCTTGGCGCCCCGCTGGTGGCTACCAACGACGTACGTTtc > 1:216515/1‑136 (MQ=255) tCCCCGAGCGCTTCTACGTTGAAGTGCAGCGCACCAATCGGGCACGCGACGAAGAATACGTGCACGCGGCGGTGGCATTGGCCGACAAGCTTGGCGCCCCGCTGGTGGCTACCAACGACGTACGTTTCATCAAGCa > 2:35942/1‑136 (MQ=255) ccccTAGCGCTTCTAGGTTTAAGTGCAGCGCACCATTCGGGCTCGCGACGAATAATAGGTGCAGGGGGCGGTGGCATTGGTCGACAAGCTTGGCGCCCCGCTGGTGGCTACCAACGACGTACGTTTCATCAAGCAg < 1:377751/136‑1 (MQ=255) ccccGAGCGCTTCTACGTTGAAGTGCAGCGCACCAATCGGGCACGCGACGAAGAATACGTGCACGCGGCGGTGGCATTGGTCGACAAGCTTGGCGCCCCGCTGGTGGCTACCAACGACGTACGTTTCATCAAGCAg < 1:100469/136‑1 (MQ=255) ccccGAGCGCTTCTACGTTGAAGTGCAGCGCACAAATCTGGAACGCGACGAAGAATACGTGCACGCGGCGGTGGCATTGGCCGACAAGCTTGTCGCCCCGCTGGTGGCTACCAACGACGTACGTTTCATCAAGCAg < 2:264306/136‑1 (MQ=255) gcTTCTATGTTGAAGTGCAGCGCACCAATCGGGCACGCGACGAAGAATACGTGCACGCGGCGGTGGCATTGGCCGACAAGCTTGGCGCCCCGCTGGTGGCTACCAACGACGTACGTTTCATCAAGCAGGCGGACtt > 1:239172/1‑136 (MQ=255) taaGGGCAGCGCACCAATCGGGCACGCGACGAAGAATACGTGCACGCGGCGGTGGCATTGGCCGACAAGCTTGGCGCCCCGCTGGTGGCTACCAACGACGTACGTTTCATCAAGCAGGCGGACTTCGACGCCCACg < 1:297530/135‑1 (MQ=255) gAAGTGCAGCGCACCAATCGGGCACGCGACGAAGAATACGTGCACGCGGCGGTGGCATTGGCCGACAAGCTTGGCGCCCCGCTGGTGGCTACCAACGACGTACGTTTCATCAAGCAGGCGGACTTCGACGCCCACg < 1:103796/136‑1 (MQ=255) aCCAATCGGGCACGCGACGAAGAATACGTGCACGCGGCGGTGGCATTGGCCGACAAGCTTGGCGCCCCGCTGGTGGCTACCAACGACGTACGTTTCATCAAGCAGGCGGACTTCGACGCCCACGAGACCCGCGTAt > 1:344351/1‑136 (MQ=255) gcgACGAAGAATACGTGCACGCGGCGGTGGCATTGGCCGACAAGCTTGGCGCCCCGCTGGTGGCTACCAACGACGTACGTTTCATCAAGCAGGCGGACTTCGACGCCCACGAGACCCGCGTATGCATTGGCGAggg < 2:20957/136‑1 (MQ=255) gaaTACGTGCACGCGGCGGTGGCATTGGCCGACAAGCTTGGCGCCCCGCTGGTGGCTACCAACGACGTACGTTTCATCAAGCAGGCGGACTTCGACGCCCACGAGACCCGCGTATGCATTGGCGAGGGATGGAccc < 2:344351/136‑1 (MQ=255) cggcggTGGCATTGGCCGACAAGCTTGGCGCCCCGCTGGTGGCTACCAACGACGTACGTTTCATCAAGCAGGCGGACTTCGACGCCCACGAGACCCGCGTATGCATTGGCGAggg < 2:196436/115‑1 (MQ=255) cggcggTGGCATTGGCCGACAAGCTTGGCGCCCCGCTGGTGGCTACCAACGACGTACGTTTCATCAAGCAGGCGGACTTCGACGCCCACGAGACCCGCGTATGCATTGGCGAggg > 1:196436/1‑115 (MQ=255) ggTGGCATTGGCCGACAAGCTTGGCGCCCCGCTGGTGGCTACCAACGACTTACGTTTCATCAAGCAGTCGGACTTCGACGCCCACTAGACCCGCTTATGCATTGGCGAGGGATGGACCCTTGACGACCCGCGCCGt < 1:323964/136‑1 (MQ=255) ggTGGCATTGGCCGACAAGCTTGGCGCCCCGCTGGTGGCTACCAACGACGTACGTTTCATCAAGCAGGCGGACTTCGACGCCCACGAGACCCGCGTATGCATTGGCGAGGGATGGACCCTTGACGACCCGCGCCGt < 1:35942/136‑1 (MQ=255) | GCGGCAAGACGAAGCCGAAGCGCTTCTTCAAGACTGGATGGGCATGTTCCCCGAGCGCTTCTACGTTGAAGTGCAGCGCACCAATCGGGCACGCGACGAAGAATACGTGCACGCGGCGGTGGCATTGGCCGACAAGCTTGGCGCCCCGCTGGTGGCTACCAACGACGTACGTTTCATCAAGCAGGCGGACTTCGACGCCCACGAGACCCGCGTATGCATTGGCGAGGGATGGACCCTTGACGACCCGCGCCGT > NC_002947/1800493‑1800745 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |