| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 1,804,865 | C→A | 17.4% | G19G (GGC→GGA) | tilS → | tRNA lysidine(34) synthetase TilS |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 1,804,865 | 0 | C | A | 17.4% | 56.6 / 6.1 | 23 | G19G (GGC→GGA) | tilS | tRNA lysidine(34) synthetase TilS |
| Reads supporting (aligned to +/- strand): ref base C (13/6); new base A (2/2); total (15/8) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 5.89e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.29e-01 | |||||||||||
GCTGCCCTTGAGGCTTGCGCCGTACGTGTGGGAGCGGGCATGCCCGCGAATGCTTTCTGGAATCGAGCGAGGGCTAGATGATCAACCTCACTCCTTGGCTCAACGCCCCCACCTGGTACGTCGCCTTCTCCGGCGGCCTCGATTCCACCGTCCTCCTGCACCTGCTGGCCGAATACGCCCGCAACCACGCATCCCCCCCGCTGCGCGCCATCCATATCCACCACGGCCTGCAAGCTGCTGCCGACGCCTGGCCCGCCCACTGCCAGGCC > NC_002947/1804732‑1805000 | gcTGCCCTTGAGGCTTGCGCCGTACGTGTGGGAGCGGGCATGCCCGCGAATGCTTTCTGGAATCGAGCGAGGGCTAGATGATCAACCTCACTCCTTGGCTCAACGCCCCCACCTGGTACGTCGCCTTCTCcggcgg > 1:175337/1‑136 (MQ=255) tGAGGCTTGCGCCGTACGTGTGGGAGCGGGCATGCCCGCGAATGCTTTCTGGAATCGAGCGAGGGCTAGATGATCAACCTCACTCCTTGGCTCAACGCCCCCACCTGGTACGTCGCCTTCTCCGGCGGCCTCGAtt < 2:468086/136‑1 (MQ=255) gcCGTACGTGTGGGAGCGGGCATGCCCGCGAATGCTTTCTGGAATCGAGCGAGGGCTAGATGATCAACCTCACTCCTTGGCTCAACGCCCCCACCTCGTACGTCGCCTTCTCCGGCGGCCTCGATTCCACCGtcct > 2:404744/1‑136 (MQ=255) gggCATGCCCGCGAATGCTTTCTGGAATCGAGCGAGGGCTAGATGATCAACCTCACTCCTTGGCTCAACGCCCCCACCTGGTACGTCGCCTTCTCCGGCGGCCTCGATTCCACCGTCCTCCTGCACCTGCTGGCCg > 2:276820/1‑136 (MQ=255) gggCATGCCCGCGAATGCTTTCTGGAATCGAGCGAGGGCTAGATGATCAACCTCACTCCTTGGCTCAACGCCCCCACCTGGTACGTCGCCTTCTCCGGCGGCCTCGATTCCACCGTCCTCCTGCACCTGCTGGCCg > 1:228936/1‑136 (MQ=255) gggCATGCCCGCGAATGCTTTCTGGAATCGAGCGAGGGCTAGATGATCAACCTCACTCCTTGGCTCAACGCCCCCACCTGGTACGTCGCCTTCTCCGGCGGCCTCGATTCCACCGTCCTCCTGCACCTGCTGGCCg > 1:292323/1‑136 (MQ=255) aTGCCCGCGAATGCTTTCTGGAATCGAGCGAGGGCTAGATGATCAACCTCACTCCTTGGCTCAACGCCCCCACCTGGTACGTCGCCTTCTCCGGCGGCCTCGATTCCACCGTCCTCCTGCACCTGCTGGCCGAATa > 1:104865/1‑136 (MQ=255) cTGGAATCGAGCGAGGGCTAGATGATCAACCTCACTCCTTGGCTCAACGCCTCCACCTGGTACGTCGCCTTCTCCGGCGGCCTCGATTCCACCGTCCTCCTGCACCTGCTGGCCGAATACGCCCGCAACCACGCAt < 2:509953/136‑1 (MQ=255) cgagcgagGGCTAGATGATCAACCTCACTCCTTGGCTCAACGCCCCCACCTGGTACGTCGCCTTCTCCGGCGGCCTCGATTCCACCGTCCTCCTGCACCTGCTGGCCGAATACGCCCGCAACCACGCATccccccc < 1:436607/136‑1 (MQ=255) gagGGCTAGATGATCAACCTCACTCCTTGGCTCAACGCCCCCACCTGGTACGTCGCCTTCTCCGGAGGCCTCGATTCCACCGTCCTCCTGCACCTGCTGGCCGAATACGCCCGCAACCACGCATCCCCCCCGCTgc > 1:124847/1‑136 (MQ=255) gagGGCTAGATGATCAACCTCACTCCTTGGCTCAACGCCCCCACCTGGTACGTCGCCTTCTCCGGAGGCCTCGATTCCACCGTCCTCCTGCACCTGCTGGCCGAATACGCCCGCAACCACGCATCCCCCCCGCTgc > 1:127349/1‑136 (MQ=255) agGGCTAGATGATCAACCTCGCTCCTTGGCTCAACGCCCCCACCTGGTACGTCGCCTTCTCCGGCGGCCTCGATTCCACCGTCCTCCTGCACCTGCTGGCCGAATACGCCCGCAACCACGCATCCCCCCCGCTgcg < 1:433067/136‑1 (MQ=255) agGGCTAGATGATCAACCTCACTCCTTGGCTCAACGCCCCCACCTGGTACGTCGCCTTCTCCGGCGGCCTCGATTCCACCGTCCTCCTGCACCTGCTGGCCGAATACGCCCGCAACCACGCATCCCCCCCGCTgcg < 2:175337/136‑1 (MQ=255) gCTAGATGATCAACCACACTCCAAGCCACAACGCCCCCACCTGGTACGTCGCCTTCTCCGGCGGCCTCGATTCCAAAGTCCTCCTGCACCTGCTGGCCGAATACGCACGCAACCACGCATCCCCCCCGCTgcgcgc > 1:228069/1‑136 (MQ=255) ccTTGGCTCAACGCCCCCACCTGGTACGTCGCCTTCTCCGGCGGCCTCGATTCCACCGTCCTCCTGCACCTGCTGGCCGAATACGCCCGCAACCACGCATCCCCCCCGCTGCGCGCCATCCATATCCACCACGGcc > 2:419661/1‑136 (MQ=255) cTTGGCTCAACGCCCCCACCTGGTACGTCGCCTTCTCCGGCGGCCTCGATTCCACCGTCCTCCTGCACCTGCTGGCCGAATACGCCCGCCACCACGCATCCCCCCCGCTGAGCGCCATCCATATCCACCACGGcca > 2:464063/1‑135 (MQ=255) cTTGGCTCAACGCCCCCACCTGGTACGTCGCCTTCTCCGGCGGCCTCGATTCCACCGTCCTCCTGCACCTGCTGGCCGAATACGCCCGCAACCACGCATCCCCCCCGCTGCGCGCCATCCATATCCACCACGGcca > 1:184012/1‑135 (MQ=255) cTTGGCTCAACGCCCCCACCTGGTACGTCGCCTTCTCCGGCGGCCTCGATTCCACCGTCCTCCTGCACCTGCTGGCCGAATACGCCCGCAACCACGCATCCCCCCCGCTGCGCGCCATCCATATCCACCACGGCCt > 1:208346/1‑136 (MQ=255) gCCCCCACCTGGTACGTCGCCTTCTCCGGCGGCCTCGATTCCACCGTCCTCCTGCACCTGCTGGCCGAATACGCCCGCAACCACGCATCCCCCCCGCTGCGCGCCATCCATATCCACCACGGCCTGCCACctgctg > 2:168403/1‑136 (MQ=255) ggTACGTCGCCTTCTCCGGCGGCCTCGATTCCACCGTCCTCCTGCACCTGCTGGCCGAATACGCCCGCAACCACGCATCCCCCCCGCTGCGCGCCATCCATATCCACCACGGCCTGCAAGCTGCTGCCGACGCCTg < 1:75599/136‑1 (MQ=255) cGTCGCCTTCTCCGGAGGCCTCGATTCCACCGTCCTCCTGCACCTGCTGGCCGAATACGCCCGCAACCACGCATCCCCCCCGCTGCGCGCCATCCATATCCACCACGGCCTGCAAGCTGCTGCCGACGCCTGgccc < 2:124847/136‑1 (MQ=255) cGTCGCCTTCTCCGGAGGCCTCGATTCCACCGTCCTCCTGCACCTGCTGGCCGAATACGCCCGCAACCACGCATCCCCCCCGCTGCGCGCCATCCATATCCACCACGGCCTGCAAGCTGCTGCCGACGCCTGgccc < 2:127349/136‑1 (MQ=255) ccTTCTCCGGCGGCCTCGATTCCACCGTCCTCCTGCACCTGCTGGCCGAATACGCCCGCAACCACGCATCCCCCCCGCTGCGCGCCATCCATATCCACCACGGCCTGCAAGCTGCTGCCGACGCCTGgcccgcccc > 1:409462/1‑135 (MQ=255) ccTTCTCCGGCGGCCTCGATTCCACCGTCCTCCTGCACCTGCTGGCCGAAAACGCCCGCAACCACGCATCCCCCCCGCTGCGCGCCATCCATATCCACCACGGCCTGCAAGCTGCTGCCGACGCCTGgcccgcccc > 1:257534/1‑135 (MQ=255) cggcCTCGATTCCACCGTCCTCCTGCACCTGCTGGCCGAATACGCCCGCAACCACGCATCCCCCCCGCTGCGCGCCATCCATATCCACCACGGCCTGCAAGCTGCTGCCGACGCCTGGCCCGCCCACTGCCAGGcc < 1:419661/136‑1 (MQ=255) | GCTGCCCTTGAGGCTTGCGCCGTACGTGTGGGAGCGGGCATGCCCGCGAATGCTTTCTGGAATCGAGCGAGGGCTAGATGATCAACCTCACTCCTTGGCTCAACGCCCCCACCTGGTACGTCGCCTTCTCCGGCGGCCTCGATTCCACCGTCCTCCTGCACCTGCTGGCCGAATACGCCCGCAACCACGCATCCCCCCCGCTGCGCGCCATCCATATCCACCACGGCCTGCAAGCTGCTGCCGACGCCTGGCCCGCCCACTGCCAGGCC > NC_002947/1804732‑1805000 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |