| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 2,601,596 | A→G | 41.7% | T324A (ACC→GCC) | PP_2277 → | hypothetical protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 2,601,596 | 0 | A | G | 41.7% | 0.1 / 9.6 | 12 | T324A (ACC→GCC) | PP_2277 | hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base A (2/5); new base G (3/2); total (5/7) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 5.58e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
GGTTCGCTGCTTATGAGAATATTAAGTCTGCACTTGATTTGTATTTCTCCGTAGCTACTGGCGCACACCGTTACACAGATGGTCGATTCCTTGCTTTGATTCAAGGTTTGGAGACCTTCCATAGAACCATTAGTGACGAAACCCTTAAAGATGAAGCTGTTTTTCGAAGAGATTTGGCTAGGGTGCTATGGAGTGCGCCAAAAGACTTGCGTAGATGGGTCCACGGCAGGACTCGCTATGG > NC_002947/2601471‑2601711 | ggTTCGCTGCTTATGAGAATATTAAGTCTGCACTTGATTTGTATTTCTCCGTAGCTACTGGCGCACACCGTTACACAGATGGTCGATTCCTTGCTTTGATTCAAGGTTTGGAGACCTTCCATAGAACCATTAGTGa < 2:235042/136‑1 (MQ=255) gTTCGCTGCTTATGAGAATATTAAGTCTGCACTTGATTTGTATTTCTCCGTAGCTACTGGCGCACACCGTTACACAGATGGTCGATTCCTTGCTTTGATTCAAGGTTTGGAGACCTTCCATAGAACCATTAGTGAc > 2:179998/1‑136 (MQ=255) tttCTCCGTAGCTACTGGCGCACACCGTTACACAGATGGTCGATTCCTTGCTTTGATTCAAGGTTTGGAGACCTTCCATAGAACCATTAGTGACGAAACCCTTAAAGATGAAGCTGTTTTTCGAAGAGATTTGGCt < 1:206473/136‑1 (MQ=255) tttCTCCGTAGCTACTGGCGCACACCGTTACACAGATGGTCGATTCCTTGCTTTGATTCAAGGTTTGGAGACCTTCCATAGAACCATTAGTGACGAAACCCTTAAAGATGAAGCTGTTTTTCGAAGAGATTTGGCt < 2:101014/136‑1 (MQ=255) tttCTCCGTAGCTACTGGCGCACACCGTTACACAGATGGTCGATTCCTTGCTTTGATTCAAGGTTTGGAGACCTTCCATAGAACCATTAGTGACGAAACCCTTAAAGATGAAGCTGTTTTTCGAAGAGATTTGGCt < 2:13011/136‑1 (MQ=255) gTCGATTCCTTGCTTTGATTCAAGGTTTGGAGACCTTCCATAGAGCCATTAGTGACGAAACCCTTAAAGATGAAGCTGTTTTTCGAAGAGATTTGGCTAGGGTGCTATGGAGTGCGCCAAAAGACTTGCGTAGATg > 1:236184/1‑136 (MQ=255) gTCGATTCCTTGCTTTGATTCAAGGTTTGGAGACCTTCCATAGAGCCATTAGTGACGAAACCCTTAAAGATGAAGCTGTTTTTCGAAGAGATTTGGCTAGGGTGCTATGGAGTGCGCCAAAAGACTTGCGTAGATg > 1:411693/1‑136 (MQ=255) gTCGATTCCTTGCTTTGATTCAAGGTTTGGAGACCTTCCATAGAGCCATTAGTGACGAAACCCTTAAAGATGAAGCTGTTTTTCGAAGAGATTTGGCTAGGGTGCTATGGAGTGCGCAAAAAGACTTGCGTAGATg > 1:493121/1‑136 (MQ=255) gTTTGGATACCATCCATAGAGCCATTATTGACGAAACCCTTAAAGATGAAGCTGTTTTTCGAAGAGATTTGGCTAGGGTGCTATGGAGTGCGCCAAAAGACTTGCGTAGATGGGTCCACGGCAGGACTCGCTATgg < 2:493121/136‑1 (MQ=255) gTTTGGAGACCTTCCATAGAGCCATTAGTGACGAAACCCTTAAAGATGAAGCTGTTTTTCGAAGAGATTTGGCTAGGGTGCTATGGAGTGCGCCAAAAGACTTGCGTAGATGGGTCCACGGCAGGACTCGCTATgg < 2:411693/136‑1 (MQ=255) agaCCTTCCATAGAACCATTAGTGACGAAACCCTTAAAGATGAAGCTGTTTTTCGAAGAGATTTGGCTAGGGTGCTATGGAGTGCGCCAAAAGACTTGCGTa < 1:284/102‑1 (MQ=255) agaCCTTCCATAGAACCATTAGTGACGAAACCCTTAAAGATGAAGCTGTTTTTCGAAGAGATTTGGCTAGGGTGCTATGGAGTGCGCCAAAAGACTTGCGTa > 2:284/1‑102 (MQ=255) | GGTTCGCTGCTTATGAGAATATTAAGTCTGCACTTGATTTGTATTTCTCCGTAGCTACTGGCGCACACCGTTACACAGATGGTCGATTCCTTGCTTTGATTCAAGGTTTGGAGACCTTCCATAGAACCATTAGTGACGAAACCCTTAAAGATGAAGCTGTTTTTCGAAGAGATTTGGCTAGGGTGCTATGGAGTGCGCCAAAAGACTTGCGTAGATGGGTCCACGGCAGGACTCGCTATGG > NC_002947/2601471‑2601711 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |