Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	2,758,975	T→C	21.7%	A394A (GCA→GCG)	PP_2411 ←	MFS transporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	2,758,975	0	T	C	21.7%	38.6 / 8.3	23	A394A (GCA→GCG)	PP_2411	MFS transporter
Reads supporting (aligned to +/- strand):  ref base T (8/10);  new base C (3/2);  total (11/12)
Fisher's exact test for biased strand distribution p-value = 6.40e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.08e-01

ACACCGTCCACACCCAGGTCGTCATGCAAAGAAATCCGCGACGTTTCCGGCAACGCCAACCCGCCCACCAACGCCACCAGCGCAATCACCACCAGGTAGAACGCCGGTGCCAGGCTGCTGCCGGTCTGTGCGATGAGCCAGGTTGCCACCAGCGGCGCTGTGCCGCCAAACAGCGTGTAGGCCACGTTGTAGGTAATCGCCGAAGCGGTGTAGCGGGTACGGGTAGGGAAGCTTTCCGACAGCAATGCTGCGGTAA  >  NC_002947/2758847‑2759102                                                                                                                                 |                                                                                                                                acacCGTCCACACCCAGGTCGTCATGCAAAGAAATCCGCGACGTTTCCGGCAACGCCAACCCGCCCACCAACGCCACCAGCGCAATCACCACCAGGTAGAACGCCGGTGCCAGGCTGCTGCCGGTCTGCGCGATGa                                                                                                                          >  2:344956/1‑136 (MQ=255)         cacaCCCAGGTCGTCATGCAAAGAAATCCGCGACGTTTCCGGCAACGCCAACCCGCCCACCAACGCCCCCAGCGCAATCACCACCAGGTAGAACGCCGGTGCCAGGCTGCTGCCGGTCTGTGCGATGAGCCAGGtt                                                                                                                  >  2:440612/1‑136 (MQ=255)         cacaCCCAGGTCGTCATGCAAAGAAATCCGCGACGTTTCCGGCAACGCCAACCCGCCCACCAACGCCACCAGCGCAATCACCACCAGGTAGAACGCCGGTGCCAGGCTGCTGCCGGTCTGTGCGATGAGCCAGGtt                                                                                                                  <  1:13773/136‑1 (MQ=255)         cacaCCCAGGTCGTCATGCAAAGAAATCCGCGACGTTTCCGGCAACGCCAACCCGCCCACCAACGCCACCAGCGCAATCACCACCAGGTAGAACGCCGGTGCCAGGCTGCTGCCGGTCTGTGCGATGAGCCAGGtt                                                                                                                  <  1:248125/136‑1 (MQ=255)         cacaCCCAGGTCGTCATGCAAAGAAATCCGCGACGTTTCCGGCAACGCCAACCCGCCCACCAACGCCACCAGCGCAATCACCACCAGGTAGAACGCCGGTGCCAGGCTGCTGCCGGTCTGTGCGATGAGCCAGCtt                                                                                                                  >  2:28050/1‑136 (MQ=255)          acacCCAGGTCGTCATGCAAAGAAATCCGCGACGTTTCCGGCAACGCCAACCCGCCCACCAACGCCACCAGCGCAATCACCACCAGGTAGAACGCCGGTGCCAGGCTGCTGCCGGTCTGCGCGATGAGCCAGGTTg                                                                                                                 >  1:266387/1‑136 (MQ=255)              ccAGGTCGTCATGCAAAGAAATCCGCGACGTTTCCGGCAACGCCAACCCGCCCACCAACGCCACCAGCGCAATCACCACCAGGTAGAACGCCGGTGCCAGGCTGCTGCCGGTCTGCGCGATGAGCCAGGTTGccac                                                                                                             >  2:245915/1‑136 (MQ=255)                           cAAAGAAATCCGCGACGTTTCCGGCAACGCCAACCCGCCCACCAACGCCACCAGCGCAATCACCACCAGGTAGAACGCCGGTGCCAGGCTGCTGCCGGTCTGTGCGATGAGCCAGGTTGCCACCAGCGGCGCtgtg                                                                                                >  1:317047/1‑136 (MQ=255)                                  aTCCGCGACGTTTCCGGCAACGCCAACCCGCCCACCAACGCCACCAGCGCAATCACCACCAGGTAGAACGCCGGTGCCAGGCTGCTGCCGGTCTGCGCGATGAGCCAGGTTGCCACCAGCGGCGCTGTGCCGCCaa                                                                                         <  1:222706/136‑1 (MQ=255)                                             ttCCGGCAACGCCAACCCGCCCACCAACGCCACCAGCGCAATCACCACCAGGTAGAACGCCGGTGCCAGGCTGCTGCCGGTCTGTGCGATGAGCCAGGTTGCCACCAGCGGCGCTGTGCCGCCAAACAGCGTGTAg                                                                              <  1:440612/136‑1 (MQ=255)                                                cGGCAACGCCAACCCGCCCACCAACGCCACCAGCGCAATCACCACCAGGTAGAACGCCGGTGCCAGGCTGCTGCCGGTCTGTGCGATGAGCCAGGTTGCCACCAGCGGCGCTGTGCCGCCAAACAGCGTGTAGGcc                                                                           <  2:78137/136‑1 (MQ=255)                                                        ccAACCCGCCCACCAACGCCACCAGCGCAATCACCACCAGGTAGAACGCCGGTGCCAGGCTGCTGCCGGTCTGTGCGATGAGCCAGGTTGCCACCAGCGGCGCTGTGCCGCCAAACAGCGTGTAGGCCACGTTGTa                                                                   <  2:356546/136‑1 (MQ=255)                                                                  caccaACGCCACCAGCGCAATCACCACCAGGTAGAACGCCGGTGCCAGGCTGCTGCCGGTCTGTGCGATGAGCCAGGTTGCCACCAGCGGCGCTGTGCCGCCAAACAGCGTGTAGGCCACGTTGTAGGTAATCGcc                                                         <  2:250055/136‑1 (MQ=255)                                                                  caccaACGCCACCAGCGCAATCACCACCAGGTAGAACGCCGGTGCCAGGCTGCTGCCGGTCTGTGCGATGAGCCAGGTTGCCACCAGCGGCGCTGTGCCGCCAAACAGCGTGTAGGCCACGTTGTAGGTAATCGcc                                                         <  2:333180/136‑1 (MQ=255)                                                                   accaACGCCACCAGCGCAATCACCACCAGGTAGAACGCCGGTGCCAGGCTGCTGCCGGTCTGTGCGATGAGCCAGGTTGCCACCAGCGGCGCTGTGCCGCCAAACAGCGTGTAGGCCACGTTGTAGGTAATCGCCg                                                        >  1:26292/1‑136 (MQ=255)                                                                   accaACGCCACCAGCGCAATCACCACCAGGTAGAACGCCGGTGCCAGGCTGCTGCCGGTCTGTGCGATGAGCCAGGTTGCCACCAGCGGCGCTGTGCCGCCAAACAGCGTGTAGGCCACGTTGTAGGTAATCGCCg                                                        >  1:23788/1‑136 (MQ=255)                                                                   accaACGCCACCAGCGCAATCACCACCAGGTAGAACGCCGGTGCCAGGCTGCTGCCGGTCTGCGCGATGAGCCAGGTTGCCACCAGCGGCGCTGTGCCGCCAAACAGCGTGTAGGCCACGTTGTAGGTAATCGCCg                                                        <  1:344956/136‑1 (MQ=255)                                                                          ccaccaGCGCAATCACCACCAGGTAGAACGCCGGTGCCAGGCTGCTGCCGGTCTGTGCGATGAGCCAGGTTGCCACCAGCGGCGCTGTGCCGCCAAACAGCGTGTAGGCCACGTTGTAGGTAATCGCCGAAGCGgt                                                 >  2:193837/1‑136 (MQ=255)                                                                                    aaTCACCACCAGGTAGAACGCCGGTGCCAGGCTGCTGCCGGTCTGTGCGATGAGCCAGGTTGCCACCAGCGGCGCTGTGCCGCCAAACAGCGTGTAGGCCACGTTGTAGGTAATCGCCGAAGCGGTGTAGcgggta                                       >  1:258049/1‑136 (MQ=255)                                                                                                       gCCGGTGCCAGGCTGCTGCCGGTCTGTGCGATGAGCCAGGTTGCCACCAGCGGCGCTGTGCCGCCAAACAGCGTGTAGGCCACGTTGTAGGTAATCGCCGAAGCGGTGTAGCGGGTACGGGTAGGGAAGCTTTCCg                    >  1:418590/1‑136 (MQ=255)                                                                                                             gCCAGGCTGCTGCCGGTCTGTGCGATGAGCCAGGTTGCCACCAGCGGCGCTGTGCCGCCAAACAGCGTGTAGGCCACGTTGTAGGTAATCGCCGAAGCGGTGTAGCGGGTACGGGTAGGGAAGCTTTCCGACAGCa              <  2:317047/136‑1 (MQ=255)                                                                                                                         ccGGTCTGTGCGATGAGCCAGGTTGCCACCAGCGGCGCTGTGCCGCCAAACAGCGTGTAGGCCACGTTGTAGGTAATCGCCGAAGCGGTGTAGCGGGTACGGGTAGGGAAGCTTTCCGACAGCAATGCTGCGGTaa  <  2:23788/136‑1 (MQ=255)                                                                                                                         ccGGTCTGTGCGATGAGCCAGGTTGCCACCAGCGGCGCTGTGCCGCCAAACAGCGTGTAGGCCACGTTGTAGGTAATCGCCGAAGCGGTGTAGCGGGTACGGGTAGGGAAGCTTTCCGACAGCAATGCTGCGGTaa  <  2:26292/136‑1 (MQ=255)                                                                                                                                 |                                                                                                                                ACACCGTCCACACCCAGGTCGTCATGCAAAGAAATCCGCGACGTTTCCGGCAACGCCAACCCGCCCACCAACGCCACCAGCGCAATCACCACCAGGTAGAACGCCGGTGCCAGGCTGCTGCCGGTCTGTGCGATGAGCCAGGTTGCCACCAGCGGCGCTGTGCCGCCAAACAGCGTGTAGGCCACGTTGTAGGTAATCGCCGAAGCGGTGTAGCGGGTACGGGTAGGGAAGCTTTCCGACAGCAATGCTGCGGTAA  >  NC_002947/2758847‑2759102

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑