| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 3,150,691 | G→A | 22.2% | V222I (GTC→ATC) | msuE → | FMN reductase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 3,150,691 | 0 | G | A | 22.2% | 30.7 / 4.9 | 18 | V222I (GTC→ATC) | msuE | FMN reductase |
| Reads supporting (aligned to +/- strand): ref base G (11/3); new base A (2/2); total (13/5) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 5.33e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.81e-01 | |||||||||||
GCCCGCTATTCGCTTTCTTCCAGGCCCACACCCTGCCGTACGGCCTGTACGCCAGTGTCGAGTCTTTCGACGATCAGCGCCTGGCTGACCCTGCGCAGTTCGAACGGATCGAGCGGGTACTCGATACCGTCGGCGCCTTCTTTCATATCCCGGTCGCCCGCGCGGCCTGACTTTCCGAGTTACAGGAGACATCGCCATGAGCCAACCGATCAAATTCGCCTACTGGGTTCCTAACGTCAGCGGTGGCCTCGTTGTC > NC_002947/3150563‑3150818 | gcCCGCTATTCGCTTTCTTCCAGGCCCACACCCTGCCGTACGGCCTGTACGCCAGTGTCGAGTCTTTCGACGATCAGCGCCTGGCTGACCCTGCGCAGTTCGAACGGATCGAGCGGGTACTCGATACCGTCGgcgc < 2:263194/136‑1 (MQ=255) cccTGCCGTACGGCCTGTACGCCAGTGTCGAGTCTTTCGACGATCAGCGCCTGGCTGACCCTGCGCAGTTCGAACGGATCGAGCGGGTACTCGATACCATCGGCGCCTTCTTTCATATCCCGGTCGCCCGCGCGGc > 1:38322/1‑136 (MQ=255) cccTGCCGTACGGCCTGTACGCCAGTGTCGAGTCTTTCGACGATCAGCGCCTGGCTGACCCTGCGCAGTTCGAACGGATCGAGCGGGTACTCGATACCATCGGCGCCTTCTTTCATATCCCGGTCGCCCGCGCGGc > 1:40785/1‑136 (MQ=255) cGTACGGCCTGTACGCCAGTGTCGAGTCTTTCGACGATCAGCGCCTGGCTGACCCTGCGCAGTTCGAACGGATCGAGCGGGTACTCGATACCGTCGGCGCCTTCTTTCATATCCCGGTCGCCCGCGCGGCCTGACt > 1:450051/1‑136 (MQ=255) cGTACGGCCTGTACGCCAGTGTCGAGTCTTTCGACGATCAGCGCCTGGCTGACCCTGCGCAGTTCGAACGGATCGAGCGGGTACTCGATACCGTCGGCGCCTTCTTTCATATCCCGGTCGCCCGCGCGGCCTGACt > 2:292852/1‑136 (MQ=255) tACGGCCTGTACGCCAGTGTCGAGTCTTTCGACGATCAGCGCCTGGCTGACCCTGCGCAGTTCGAACGGATCGAGCGGGTACTCGATACCATCGGCGCCTTCTTTCATATCCCGGTCGCCCGCGCGGCCTGACttt < 2:40785/136‑1 (MQ=255) tACGGCCTGTACGCCAGTGTCGAGTCTTTCGACGATCAGCGCCTGGCTGACCCTGCGCAGTTCGAACGGATCGAGCGGGTACTCGATACCATCGGCGCCTTCTTTCATATCCCGGTCGCCCGCGCGGCCTGACttt < 2:38322/136‑1 (MQ=255) aCGCCAGTGTCGAGTCTTTCGACGATCAGCGCCTGGCTGACCCTGCGCAGTTCGAACGGATCGAGCGGGTACTCGATACCGTCGGCGCCTTCTTTCATATCCCGGTCGCCCGCGCGGCCTGACTTTCCGAGTTACa > 2:414990/1‑136 (MQ=255) cAGTGTCGAGTCTTTCGACGATCAGCGCCTGGCTGACCCTGCGCAGTTCGAACGGATCGAGCGGGTACTCGATACCGTCGGCGCCTTCTTTCATATCCCGGTCGCCCGCGCGGCCTGACTTTCCGAGTTACAGgag < 1:126065/136‑1 (MQ=255) gtCGAGTCTTTCGACGATCAGCGCCTGGCTGACCCTGCGCAGTTCGAACGGATCGAGCGGGTACTCGATACCGTCGGCGCCTTCTTTCATATCCCGGTCGCCCGCGCGGCCTGACTTTCCGAGTTACAGGAGACAt > 2:460165/1‑136 (MQ=255) cTTTCGACGATCAGCGCCTGGCTGACCCTGCGCAGTTCGAACGGATCGAGCGGGTACTCGATACCGTCGGCGCCTTCTTTCATATCCCGGTCGCCCGCGCGGCCAGACTTTCCGACTTACAGGAGACATAGCCATg > 1:326494/1‑136 (MQ=255) cgcAGTTCGAACGGATCGAGCGGGTACTCGATAGCGTCGGCGCCTTCTTTCATATCCCGGTCGCCCGCGCGGCCTGACTTTCCGAGTTACAGGAGACATCGCCATGAGCCAACCGATCAAATTCGCCTACTGGGtt < 1:258273/136‑1 (MQ=255) aGTTCGAACGGATCGAGCGGGTACTCGATACCGTCGGCGCCTTCTTTCATATCCCGGTCGCCCGCGCGGCCTGACTTTCCGAGTTTCAGGAGACATCGCCATGAGCCAACCGATCAAATTAGCCTACTGGGTTGCt > 2:305273/1‑136 (MQ=255) aGTTCGAACGGATCGAGCGGGTACTCGATACCGTCGGCGCCTTCTTTCATATCCCGGTCGCCCGCGCGGCCTGACTTTCCGAGTTACAGGAGACATCGCCATGAGCCAACCGATCAAATTCGCCTACTGGGTTCCt > 1:443805/1‑136 (MQ=255) aGCGGGTACTCGATACCGTCGGCGCCTTCTTTCATATCCCGGTCGCCCGCGCGGCCTGACTTTCCGAGTTACAGGAGACATCGCCATGAGCCAACCGATCAAATTCGCCTACTGGGTTCCTAACGTCAGCGGTggg > 2:378843/1‑135 (MQ=255) aGCGGGTACTCGATACCGTCGGCGCCTTCTTTCATATCCCGGTCGCCCGCGCGGCCTGACGTTCCGAGTTACAGGAGACCTCGCCATGAGCCAACCGCTCAAATTCGCCTTCTGGGTTCCTAACGTCAGCGGTGGc > 1:174880/1‑136 (MQ=255) tCGATACCGTCGGCGCCTTCTTTCATATCCCGGTCGCCCGCGCGGCCTGACTTTCCGAGTTACAGGAGACATCGCCATGAGCCAACCGATCAAATTCGCCTACTGGGTTCCTAACGTCAGCGGTGGCCTCGTTGTc > 2:316354/1‑136 (MQ=255) tCGATACCGTCGGCGCCTTCTTTCATATCCCGGTCGCCCGCGCGGCCTGACATTACGAGTTAAAGGAGACATAGCCATGAGCCAACCGAACAAATTAGCCTAAAGGGTTACTAACGTAAGCGGTTGCCTCGttttt > 2:405324/1‑133 (MQ=255) | GCCCGCTATTCGCTTTCTTCCAGGCCCACACCCTGCCGTACGGCCTGTACGCCAGTGTCGAGTCTTTCGACGATCAGCGCCTGGCTGACCCTGCGCAGTTCGAACGGATCGAGCGGGTACTCGATACCGTCGGCGCCTTCTTTCATATCCCGGTCGCCCGCGCGGCCTGACTTTCCGAGTTACAGGAGACATCGCCATGAGCCAACCGATCAAATTCGCCTACTGGGTTCCTAACGTCAGCGGTGGCCTCGTTGTC > NC_002947/3150563‑3150818 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |