| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 264,056 | C→T | 45.4% | D269D (GAC→GAT) | gabD‑I → | succinate‑semialdehyde dehydrogenase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 264,056 | 0 | C | T | 45.4% | 2.3 / 24.8 | 22 | D269D (GAC→GAT) | gabD‑I | succinate‑semialdehyde dehydrogenase |
| Reads supporting (aligned to +/- strand): ref base C (6/6); new base T (4/6); total (10/12) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.91e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.73e-01 | |||||||||||
CGACCGAAATCGGTCGCCAGCTGATGGAAGAATGCGCCAAGGACATCAAGAAGGTTTCCCTGGAGCTGGGTGGCAACGCCCCGTTCATCGTGTTCGACGACGCCGACCTGGACAAGGCGGTCGAGGGCGCGATCATCTCCAAGTACCGTAACAACGGCCAGACCTGCGTCTGCGCCAACCGTATCTACGTGCAGGACGGCGTCTACGACGCGTTCGCCGAGAAGCTGGCC > NC_002947/263950‑264179 | cGACCGAAATCGGTCGCCAGCTGATGGAAGAATGCGCCAAGGACATCAAGAAGGTTTCCCTGGAGCTGGGTGGCAACGCCCCGTTCATCGTGTTCGACGACGCCGACCTGGACAAGGCGGTCGAGGGCGCGatcat > 1:439623/1‑136 (MQ=255) ccAGCTGATGGAAGAATGCGCCAAGGACATCAAGAAGGTTTCCCTGGAGCTGGGTGGCAACGCCCCGTTCATCGTGTTCGACGACGCCGACCTGGACAAGGCGGTCGAGGGCGCGATCATCTCCAAGTACCGTaac > 1:252345/1‑136 (MQ=255) gcgcCAAGGACATCAAGAAGGTTTCCCTGGAGCTGGGTGGCAACGCCCCGTTCATCGTGTTCGACGACGCCGACCTGGAc < 2:234681/80‑1 (MQ=255) gcgcCAAGGACATCAAGAAGGTTTCCCTGGAGCTGGGTGGCAACGCCCCGTTCATCGTGTTCGACGACGCCGACCTGGAc > 1:234681/1‑80 (MQ=255) cgcCAAGGACATCAAGAAGGTTTCCCTGGAGCTGGGTGGCAACGCCCCGTTCATCGTGTTCGACGACGCCGACCTGGACAAGGCGGTCGAGGGCGCGATCATCTCCAAGAACCGTAACAACGGCCAGACCTGCgaa > 1:510322/1‑134 (MQ=255) gcCAAGGACATCAAGAAGGTTTCCCTGGAGCTGGGTGGCAACGCCCCGTTCATCGTGTTCGACGACGCCGACCTGGACAAGGCGGTCGAGGGCGCGATCATCTCCAAGTACCGTAACAACGGCCAGACCTGCGTCt < 1:469427/136‑1 (MQ=255) gccgaccATCAAGAAGCTTGGGCTTGAGCTTGGCGGCAACGCGCCTTTCATCGTGTTCGACGACGCCGATCTCGATGCCGCCGTCGAAGGTGCTCTTATCGCCAAATTCCGCAACAACGGCCAAACCTGCGTCTgc < 2:124196/130‑1 (MQ=255) cAAGGACATCAAGAAGGTTTCCCTGGAGCTGGGTGGCAACGCCCCGTTCATCGTGTTCGACGACGCCGACCTGGACAAGGCGGTCGAGGGCGCGATCATCTCCAAGTACCGTAACAACGGCCAGACCTGCGTCTgc < 2:51321/136‑1 (MQ=255) cgaccATCAAGAAGCTTGGGCTTGAGCTTGGCGGCAACGCGCCTTTCATCGTGTTCGACGACGCCGATCTCGATGCCGCCGTCGAAGGTGCTCTTATCGCCAAATTCCGCAACAACGGCCAAACCTGCGTCTgcgc < 1:268263/132‑1 (MQ=255) tCAAGAAGGTTTCCCTGGAGCTGGGTGGCAACGCCCCGTTCATCGTGTTCGACGACGCCGACCTGGACAAGGCGGTCGAGGGCGCGATCATCTCCAAGTACCGTAACAACGGCCAGACCTGCGTCTGCGCCAACCg < 2:362394/136‑1 (MQ=255) tCAAGAAGGTTTCCCTGGAGCTGGGTGGCAACGCCCCGTTCATCGTGTTCGACGACGCCGACCTGGACAAGGCGGTCGAGGGCGCGATCATCTCCAAGTACCGTAACAACGGCCAGACCTGCGTCTGCGCCAACCg < 2:442516/136‑1 (MQ=255) tCAAGAAGCTTGGGCTTGAGCTTGGCGGCAACGCGCCTTTCATCGTGTTCGACGACGCCGATCTCGATGCCGCCGTCGAAGGTGCTCTTATCGCCAAATTCCGCAACAACGGCCAAACCTGCGTCTGCGCCAACCg < 2:94062/136‑1 (MQ=255) tCAAGAAGCTTGGGCTTGAGCTTGGCGGCAACGCGCCTTTCATCGTGTTCGACGACGCCGATCTCGATGCCGCCGTCGAAGGTGCTCTTATCGCCAAATTCCGCAACAACGGCCAAACCTGCGTCTGCGCCAACCg < 1:273245/136‑1 (MQ=255) cccTGGAGCTGGGTGGCAACGCCCCGTTCATCGTGTTCGACGACGCCGACCTGGACAAGGCGGTCGAGGGCGCGATCATCTCCAAGTACCGTAACAACGGCCAGACCTGCGTCTGCGCCAACCGTATCTACGTGCa > 1:354944/1‑136 (MQ=255) gcttgAGCTTGGCGGCAACGCGCCTTTCATCGTGTTCGACGACGCCGATCTCGATGCCGCCGTCGAAGGTGCTCTTATCGCCAAATTCCGCAACAACGGCCAAACCTGCGTCTGCGCCAACCGTCTCTATGTACAg < 1:439697/132‑1 (MQ=255) gcttgAGCTTGGCGGCAACGCGCCTTTCATCGTGTTCGACGACGCCGATCTCGATGCCGCCGTCGAAGGTGCTCTTATCGCCAAATTCCGCAACAACGGCCAAACCTGCGTCTGCGCCAACCGTCTCTATGTACAg > 2:401591/5‑136 (MQ=255) tGGAGCTGGGTGGCAACGCCCCGTTCATCGTGTTCGACGACGCCGACCTGGACAAGGCGGTCGAGGGCGCGATCATCTCCAAGTACCGTAACAACGGCCAGACCTGCGTCTGCGCCAACCGTATCTACGTGCAGGa < 2:472063/136‑1 (MQ=255) gAGCTTGGCGGCAACGCGCCTTTCATCGTGTTCGACGACGCCGATCTCGATGCCGCCGTCGAAGGTGCTCTTATCGCCAAATTCCGCAACAACGGCCAAACCTGCGTCTGCGCCAACCGTCTCTATGTACAGGACg < 2:490748/136‑1 (MQ=255) ggCAACGCGCCTTTCATCGTGTTCGACGACGCCGATCTCGATGCCGCCGTCGAAGGTGCTCTTATCGCCAAATTCCGCAACAACGGCCAAACCTGCGTCTGCGCCAACCGTCTCTATGTACAGGACGGCGTCTatg > 2:275773/1‑134 (MQ=255) ggCAACGCGCCTTTCATCGTGTTCGACGACGCCGATCTCGATGCCGCCGTCGAAGGTGCTCTTATCGCCAAATTCCGCAACAACGGCCAAACCTGCGTCTGCGCCAACCGTCTCTATGTACAGGACGGCGTCTatg > 2:248918/1‑134 (MQ=255) cGTTCATCGTGTTCGACGACGCCGACCTGGACAAGGCGGTCGAGGGCGCGATCATCTCCAAGTACCGTAACAACGGCCAGACCTGCGTCTGCGCCCACCGTATCTACGTGCAGGCCGGCGTCTACGACGCGTTCGc > 2:88661/1‑136 (MQ=255) cgacgaCGCCGATCTCGATGCCGCCGTCGAAGGTGCTCTTATCGCCAAATTCCGCAACAACGGCCAAACCTGCGTCTGCGCCAACCGTCTCTATGTACAGGACGGCGTCTATGATGCCTTTGCCGAGAAGCtctcc > 1:401379/1‑132 (MQ=255) | CGACCGAAATCGGTCGCCAGCTGATGGAAGAATGCGCCAAGGACATCAAGAAGGTTTCCCTGGAGCTGGGTGGCAACGCCCCGTTCATCGTGTTCGACGACGCCGACCTGGACAAGGCGGTCGAGGGCGCGATCATCTCCAAGTACCGTAACAACGGCCAGACCTGCGTCTGCGCCAACCGTATCTACGTGCAGGACGGCGTCTACGACGCGTTCGCCGAGAAGCTGGCC > NC_002947/263950‑264179 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |