| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 3,758,459 | C→T | 21.0% | S645S (AGC→AGT) | PP_3319 → | GGDEF domain‑containing sensory box protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 3,758,459 | 0 | C | T | 21.0% | 36.0 / 4.9 | 19 | S645S (AGC→AGT) | PP_3319 | GGDEF domain‑containing sensory box protein |
| Reads supporting (aligned to +/- strand): ref base C (8/7); new base T (2/2); total (10/9) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.72e-01 | |||||||||||
GGAGATAACCGAGTCACTCTTGCTGCATAACGTTGAACAAGCAATCGCAACCTTGCACCGCCTCAAAGACTTGGGTGTACGCATTGCGCTGGATGATTTCGGTACGGGGTTCTCCAGCCTGAGTTATCTCAAGTGCTTGCCAATAGACAAAATCAAGATTGACCGCTCCTTTATCCAGGACATCTCGACGAACCCACACGATGCAGCAATCACTCAAGGCATCATTTCGATGGCCCATCATC > NC_002947/3758342‑3758583 | ggAGATAACCGAGTCACTCTTGCTGCATAACGTTGAACAAGCAATCGCAACCTTGCACCGCCTCAAAGACTTGGGTGTACGCATTGCGCTGGATGATTTCGGTACGGGGTTCTCCAGCCTGAGTTATCTCAAGTGc > 2:130939/1‑136 (MQ=255)ggAGATAACCGAGTCACTCTTGCTGCATAACGTTGAACAAGCAATCGCAACCTTGCACCGCCTCAAAGACTTGGGTGTACGCATTGCGCTGGATGATTTCGGTACGGGGTTCTCCAGCCTGAGTTATCTCAAGAGc > 2:227831/1‑136 (MQ=255) tAACCGAGTCACTCTTGCTGCATAACGTTGAACAAGCAATCGCAACCTTGCACCGCCTCAAAGACTTGGGTGTACGCATTGCGCTGGATGATTTCGGTACGGGGTTCTCCAGCCTGAGTTATCTCAAGTGCTTGcc > 2:349881/1‑136 (MQ=255) cGAGTCACTCTTGCTGCATAACGTTGAACAAGCAATCGCAACCTTGCACCGCCTCAAAGACTTGGGTGTACGCATTGCGCTGGATGATTTCGGTACGGGGTTCTCCAGCCTGAGTTATCTCAAGTGCTTGCCAATa < 1:208973/136‑1 (MQ=255) cACTCTTGCTGCATAACGTTGAACAAGCAATCGCAACCTTGCACCGCCTCAAAGACTTGGGTGTACGCATTGCGCTGGATGATTTCGGTACGGGGTTCTCCAGCCTGAGTTATCTCAAGTGCTTGCCAATAGACaa > 1:157762/1‑136 (MQ=255) ctctTGCTGCATAACGTTGAACGAGCAATCGCAACCTTGCACCGCCTCAAAGACTTGGGTGTACGCATTGCGCTGGATGATTTCGGTACGGGGTTCTCCAGTCTGAGTTATCTCAAGTGCTTGCCAATAGACaaaa > 1:101374/1‑136 (MQ=255) ctctTGCTGCATAACGTTGAACGAGCAATCGCAACCTTGCACCGCCTCAAAGACTTGGGTGTACGCATTGCGCTGGATGATTTCGGTACGGGGTTCTCCAGTCTGAGTTATCTCAAGTGCTTGCCAATAGACaaaa > 1:98924/1‑136 (MQ=255) aTAACGTTGAACGAGCAATCGCAACCTTGCACCGCCTCAAAGACTTGGGTGTACGCATTGCGCTGGATGATTTCGGTACGGGGTTCTCCAGTCTGAGTTATCTCAAGTGCTTGCCAATAGACAAAATCAAGATTGa < 2:98924/136‑1 (MQ=255) aTAACGTTGAACGAGCAATCGCAACCTTGCACCGCCTCAAAGACTTGGGTGTACGCATTGCGCTGGATGATTTCGGTACGGGGTTCTCCAGTCTGAGTTATCTCAAGTGCTTGCCAATAGACAAAATCAAGATTGa < 2:101374/136‑1 (MQ=255) gTTGAACAAGCAATCGCAACCTTGCACCGCCTCAAAGACTTGGGTGTACGCATTGCGCTGGATGATTTCGGTACGGGGTTCTCCAGCCTGAGTTATCTCAAGTGCTTGCCAATAGACAAAATCAAGATTGACCGCt < 1:138886/136‑1 (MQ=255) gTTGAACAAGCAATCGCAACCTTGCACCGCCTCAAAGACTTGGGTGTACGCATTGCGCTGGATGATTTCGGTACGGGGTTCTCCAGCCTGAGTTATCTCAAGTGCTTGCCAATAGACAAAATCAAGATTGACCGCt < 1:505988/136‑1 (MQ=255) gTTGAACAAGCAATCGCAACCTTGCACCGCCTCAAAGACTTGGGTGTACGCATTGCGCTGGATGATTTCGGTACGGGGTTCTCCAGCCTGAGTTATCTCAAGTGCTTGCCAATAGACAAAATCAAGATTGACCGCt < 1:394438/136‑1 (MQ=255) aTCGCAACCTTGCACCGCCTCAAAGACTTGGGTGTACGCATTGCGCTGGATGATTTCGGTACGGGGTTCTCCAGCCTGAGTTATCTCAAGTGCTTGCCAATAGACAAAATCAAGATTGACCGCTCCTTTATCCAgg > 2:123775/1‑136 (MQ=255) aCCTTGCACCGCCTCAAAGACTTGGGTGTACGCATTGCGCTGGATGATTTCTGTACGGGGTTCTCCAGCCTGAGTTATCTCAAGTGCTTGCCAATAGACAAAATCAAGATTGACCTCTCCTTTATCCATGACAtct < 1:34740/136‑1 (MQ=255) gCCTCAAAGACTTGGGTGTACGCATTGCGCTGGATGATTTCGGTACGGGGTTCTCCAGCCTGAGTTATCTCAAGTGCTTGCCAATAGACAAAATCAAGATTGACCGCTCCTTTATCCAGGACATCTCGACGAAccc < 2:430529/136‑1 (MQ=255) cAAAGACTTGGGTGTACGCATTGCGCTGGATGATTTCGGTACGGGGTTCTCCAGCCTGAGTTATCTCAAGTGCTTGCCAATAGACAAAATCAAGATTGACCGCTCCTTTATCCAGGACATCTCGACGAACCCacac > 2:365048/1‑136 (MQ=255) gtgtACGCATTGCGCTGGATGATTTCGGTACGGGGTTCTCCAGCCTGAGTTATCTCAAGTGCTTGCCAATAGACAAAATCAAGATTGACCGCTCCTTTATCCAGGACATCTCGACGAACCCACACGATGCAGCAAt > 2:88734/1‑136 (MQ=255) gatTTCGGTTCGGGGTTCTCCAGCCTGAGTTATCTCAAGTGCTTGCCAATAGACAAAATCAAGATTGACCGCTCCTTTATCCAGGACATCTCGACGAACCCACACGATGCAGCAATCACTCAAGGCATCATTTCGa > 2:8285/1‑136 (MQ=255) gggTTCTCCAGCCTGAGTTATCTCAAGTGCTTGCCAATAGACAAAATCAAGATTGACCGCTCCTTTATCCAGGACATCTCGACGAACCCACACGATGCAGCAATCACTCAAGGCATCATTTCGATGGCCCatcatc < 2:25735/136‑1 (MQ=255) | GGAGATAACCGAGTCACTCTTGCTGCATAACGTTGAACAAGCAATCGCAACCTTGCACCGCCTCAAAGACTTGGGTGTACGCATTGCGCTGGATGATTTCGGTACGGGGTTCTCCAGCCTGAGTTATCTCAAGTGCTTGCCAATAGACAAAATCAAGATTGACCGCTCCTTTATCCAGGACATCTCGACGAACCCACACGATGCAGCAATCACTCAAGGCATCATTTCGATGGCCCATCATC > NC_002947/3758342‑3758583 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |