| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 3,824,314 | C→G | 100% | A279P (GCG→CCG) | kguT ← | 2‑ketogluconate transporter, putative |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 3,824,314 | 0 | C | G | 87.5% | 39.4 / ‑0.2 | 16 | A279P (GCG→CCG) | kguT | 2‑ketogluconate transporter, putative |
| Reads supporting (aligned to +/- strand): ref base C (2/0); new base G (5/9); total (7/9) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.75e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.51e-01 | |||||||||||
| Rejected as polymorphism: E-value score below prediction cutoff. | |||||||||||
| Rejected as polymorphism: Variant not supported by required number of reads on each strand. | |||||||||||
TGCTCGCTGCCCAAGGCGTAGGAACCATAGAACGCCAACGCGGCAATCAGCAGCGGCGGCCAGACGAAGCGCTTGCGCTTTTGCAGGCGGTCGGACGCCCAGGACACGCCCACCATTGCCAGCACCGCCGCCAGGTAAGGCACCGAGGCCAGCCAGCCGGCCTGCACGATATCGACGTTCGCCGCCTGCTTGAGGATCGACGGCAGCCACAGCACGAAGCCATACACGCCGATGCTCCAGCAGAAGTACTGCAGCGCCAGGAT > NC_002947/3824184‑3824446 | tGCTCGCTGCCCAAGGCGTAGGAACCATAGAACGCCAACGCGGCAATCAGCAGCGGCGGCCAGACGAAGCGCTTGCGCTTTTGCAGGCGGTCGGACGCCCAGGACACGCCCACCATTGCCAGCACCGCCGGCAGGt < 2:485801/136‑1 (MQ=255) ggAACCATAGAACGCCAACGCGGCAATCAGCAGCGGCGGCCAGACGAAGCGCTTGCGCTTTTGCAGGCGGTCGGACGCCCAGGACACGCCCACCATTGCCAGCACCGCCGGCAGGTAAGGCACCGAGGccagccag < 1:217196/136‑1 (MQ=255) aaCCATAGAACGCCAACGCGGCAATCAGCAGCGGCGGCCAGACGAAGCGCTTGCGCTTTTGCAGGCGGTCGGACGCCCAGGACACGCCCACCATTGCCAGCACCGCCGGCAGGTAAGGCACCGAGGCCagccagcc > 1:164503/1‑136 (MQ=255) aaCCATAGAACGCCAACGCGGCAATCAGCAGCGGCGGCCAGACGAAGCGCTTGCGCTTTTGCAGGCGGTCGGACGCCCAGGACACGCCCACCATTGCCAGCACCGCCGGCAGGTAAGGCACCGAGGCCagccagcc > 2:270219/1‑136 (MQ=255) aaCCATAGAACGCCAACGCGGCAATCAGCAGCGGCGGCCAGACGAAGCGCTTGCGCTTATGCAGGCGGTCGGACGCCCAGGACACGCCCACCATTCCCAGCACCGCCGGCAGGTAAGGCACCGAGGCCagccagcc > 1:169691/1‑136 (MQ=255) gCAATCAGCAGCGGCGGCCAGACGAAGCGCTTGCGCTTTTGCAGGCGGTCGGACGCCCAGGACACGCCCACCATTGCCAGCACCGCCGGCAGGTAAGGCACCGAGGCCAGCCAGCCGGCCTGCACGATATCGACGt < 1:153301/136‑1 (MQ=255) aGACGAAGCGCTTGCGCTTTTGCAGGCGGTCGGACGCCCAGGACACGCCCACCATTGCCAGCACCGCCGGCAGGTAAGGCACCGAGGCCAGCCAGCCGGCCTGCACGATATCGACGTTCGCCGCCTGCTTGAGGAt > 1:79470/1‑136 (MQ=255) tgcgcttgggcttTTGCTGGCGGTCGGACGCCCTGGTCACGCCCACCATTGCCAGCACGGCCGGCAGGTAAGGCACGGAGGCCAGCCTGCCGGCCTGCACGTTATCGACGTTCGCCGCCTGCTTGAGGATCGACgg < 1:58874/135‑1 (MQ=255) gcttgcgcttTTGCAGGCGGTCGGACGCCCAGGACACGCCCACCATTGCCAGCACCGCCGGCAGGTAAGGCACCGAGGCCAGCCAGCCGGCCTGCACGATATCGACGTTCGCCGCCTGCTTGAGGATCGACGGCAg < 2:248526/136‑1 (MQ=255) ttgcgcttTTGCAGGCGGTCGGACGCCCAGGACACGCCCACCATTGCCAGCACCGCCGGCAGGTAAGGCACCGAGGCCAGCCAGCCGGCCTGCACGATATCGACGTTCGCCGCCTGCTTGAGGATCGACGGCAGcc < 2:448396/136‑1 (MQ=255) cttTTGCAGGCGGTCGGACGCCCAGGACACGCCCACCATTGCCAGCACCGCCGGCAGGTAAGGCACCGAGGCCAGCCAGCCGGCCTGCACGATATCGACGTTCGCCGCCTGCTTGAGGATCGACGGCAGCCACAGc < 1:161/136‑1 (MQ=255) ttGCAGGCGGTCGGACGCCCAGGACACGCCCACCATTGCTAGCACCGCCGGCAGGTAAGGCACCGAGGCCAGCCAGCCGGCCTGCACGATATCGACGTTCGCCGCCTGCTTGAGGATCGACGGCAGCCACAGCACg < 2:117059/136‑1 (MQ=255) cAGCACACGCCCACCATTGCCAGCACCGCCGGCAGGTAAGGCACCGAGCCCAGCCAGCCGGCCTGCACGATATCGACGTTCGCCGCCTGCTTGAGGATCGACGGCAGCCACAGCACGAAGCCATACACGCCGATGc < 1:33471/136‑1 (MQ=255) caccaTTGCCAGCACCGCCGCCAGGTAAGGCACCGAGGCCAGCCAGCCGGCCTGCACGATATCGACGTTCGCCGCCTGCTTGAGGATCGACGGCAGCCACAGCACGAAGCCATACACGCCGGTGCTCCAGCAGAAg > 2:22258/1‑136 (MQ=255) ttGCCAGCACCGCCGGCAGGTAAGGCACCGAGGCCAGCCAGCCGGCCTGCACGATATCGACGTTCGCCGCCTGCTTGAGGATCGACGGCAGCCACAGCACGAAGCCATACACGCCGATGCTCCAGCAGAAGTACTg > 2:19166/1‑136 (MQ=255) ccAGCACCGCCGCCAGGTAAGGCACCGAGGCCAGCCAGCCGGCCTGCACGATATCGACGTTCGCCGCCTGCTTGAGGATCGACGGCAGCCACAGCACGAAGCCATACACGCCGGTGCTCCAGCAGAAGTACTGCAg > 1:474372/1‑136 (MQ=255) ccggcAGGTAAGGCACCGAGGCCAGCCAGCCGGCCTGCACGATATCGACGTTCGCCGCCTGCTTGAGGATCGACGGCAGCCACAGCACGAAGCCATACACGCCGATGCTCCAGCAGAAGTACTGCAGCGCCAGgat < 2:130708/136‑1 (MQ=255) | TGCTCGCTGCCCAAGGCGTAGGAACCATAGAACGCCAACGCGGCAATCAGCAGCGGCGGCCAGACGAAGCGCTTGCGCTTTTGCAGGCGGTCGGACGCCCAGGACACGCCCACCATTGCCAGCACCGCCGCCAGGTAAGGCACCGAGGCCAGCCAGCCGGCCTGCACGATATCGACGTTCGCCGCCTGCTTGAGGATCGACGGCAGCCACAGCACGAAGCCATACACGCCGATGCTCCAGCAGAAGTACTGCAGCGCCAGGAT > NC_002947/3824184‑3824446 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |