| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 3,824,717 | C→A | 70.1% | M144I (ATG→ATT) | kguT ← | 2‑ketogluconate transporter, putative |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 3,824,717 | 0 | C | A | 70.1% | 15.7 / 9.4 | 15 | M144I (ATG→ATT) | kguT | 2‑ketogluconate transporter, putative |
| Reads supporting (aligned to +/- strand): ref base C (2/2); new base A (5/5); total (8/7) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
CAGATCACGGCAGGCAGGCCTTCGATGATGAACATCCAGCGCCAGTCGTAATGCTTGATCAGGTAACCGGACACCACCGACATCCACAGAATGGTCACCGGGTTGCCCAGCATCAGGAAGGTGTTGGCGCGTGAACGCTCGGCCCGGGTGAACCAATGGCACAGGTACACCAGCATGGCCGGCATGACCGCAGCCTCCACCACCCCGAGCAGGAAGCGGATGCCGATCAGCAT > NC_002947/3824607‑3824839 | cAGATCACGGCAGGCAGGCCTTCGATGATGAACATCCAGCGCCAGTCGTAATGCTTGATCAGGTAACCGGACACCACCGACATCCACAGAATGGTCACCGGGTTGCCCAGAATCAGGAAGGTGTTGGCGCGTGAAc < 1:183784/136‑1 (MQ=255) aggcaggcCTTCGATGATGAACATCCAGCGCCAGTCGTAATGCTTGATCAGGTAACCGGACACCACCGACATCCACAGAATGGTCACCGGGTTGCCCAGAATCAGGAAGGTGTTGGCGCGTGAACGCTCGGCCCgg > 1:447520/1‑136 (MQ=255) aggcaggcCTTCGATGATGAACATCCAGCGCCAGTCGTAATGCTTGATCAGGTAACCGGACACCACCGACATCCACAGAATGGTCACCGGGTTGCCCAGAATCAGGAAGGTGTTGGCGCGTGAACGCTCGGCCCgg > 1:51345/1‑136 (MQ=255) ttCGATGATGAACATCCAGCGCCAGTCGTAATGCTTGATCAGGTAACCGGACACCACCGACATCCACAGAATGGTCACCGGGTTGCCCAGAATCAGGAAGGTGTTGGCGCGTGAACGCTCGGCCCGGGTGAACCaa > 2:312218/1‑136 (MQ=255) gtCGCTGATGAACATCCAGCGCCAGTCGTAATGCTTGATCAGGGAACCGGACACCACCGACATCCACAGACTGGTCACCGGGTTGCCCAGCATCAGGAAGGTGTTGGCGCGTGAACGCTCGGCCCGGGTGAACCaa < 2:474372/135‑1 (MQ=255) tCGATGATGAACATCCAGCGCCAGTCGTAATGCTTGATCAGGTAACCGGACACCACCGACATCCACAGAATGGTCACCGGGTTGCCCAGAATCAGGAAGGTGTTGGCGCGTGAACGCTCGGCCCGGGTGAACCAAt < 2:447520/136‑1 (MQ=255) tCGATGATGAACATCCAGCGCCAGTCGTAATGCTTGATCAGGTAACCGGACACCACCGACATCCACAGAATGGTCACCGGGTTGCCCAGAATCAGGAAGGTGTTGGCGCGTGAACGCTCGGCCCGGGTGAACCAAt < 2:51345/136‑1 (MQ=255) cATCCAGCGCCAGTCGTAATGCTTGATCAGGTAACCGGACACCACCGACATCCACAGAATGGTCACCGGGTTGCCCAGTATCAGGAAGGTGTTGGCGCGTGAACGCTCGGCCCGGGTGAACCAATGGCACAGGTac > 1:111930/1‑136 (MQ=255) attcTTGATCAGGTAACCGGACACCACCGACATCCACAGAATGGTCACCGGGTTGCCCAGAATCAGGAAGGTGTTGGCGCGTGAACGCTCGGCCCGGGTGAACCAATGGCACAGGTACACCAGCATGGCCGGCATg < 1:289935/133‑1 (MQ=255) cAGGTAACCGGACACCACCGACATCCACAGAATGGTCACCGGGTTGCCCAGCATCAGGAAGGTGTTGGCGCGTGAACGCTCGGCCCGGGTGAACCaa < 1:4701/97‑1 (MQ=255) cAGGTAACCGGACACCACCGACATCCACAGAATGGTCACCGGGTTGCCCAGCATCAGGAAGGTGTTGGCGCGTGAACGCTCGGCCCGGGTGAACCaa > 2:4701/1‑97 (MQ=255) acaGAATGGTCACCGGGTTGCCCAGAATCAGGAAGGTGTTGGCGCGTGAACGCTCGGCCCGGGTGAACCAATGGCACAGGTACACCAGCATGGCCGGCATGACCGCAGCCTCCACCACCCCGAGCAGGAAGCGGAt < 2:239374/136‑1 (MQ=255) gTCACCGGGTTGCCCAGAATCAGGAAGGTGTTGGCGCGTGAACGCTCGGCCCGGGTGAACCAATGGCACAGGTACACCAGCATGGCCGGCATGACCGCAGCCTCCACCACCCCGAGCAGGAAGCGGATGCCGATCa > 1:80929/1‑136 (MQ=255) ccGGGTTGCCCAGCACCAGGAAGGTGTTGGCGCGTGAACGCTCGGCCCGGGTGAACCAATGGCACAGGTACACCAGCATGGCCGGCATGACCGCAGCCTCCACCACCCCGAGCAGGAAGCGGATGCCGATCAGCAt > 1:318897/1‑136 (MQ=255) ccGGGTTGCCCAGAATCAGGAAGGTGTTGGCGCGTGAACGCTCGGCCCGGGTGAACCAATGGCACAGGTACACCAGCATGGCCGGCATGACCGCAGCCTCCACCACCCCGAGCAGGAAGCGGATGCCGATCAGCAt > 1:216344/1‑136 (MQ=255) | CAGATCACGGCAGGCAGGCCTTCGATGATGAACATCCAGCGCCAGTCGTAATGCTTGATCAGGTAACCGGACACCACCGACATCCACAGAATGGTCACCGGGTTGCCCAGCATCAGGAAGGTGTTGGCGCGTGAACGCTCGGCCCGGGTGAACCAATGGCACAGGTACACCAGCATGGCCGGCATGACCGCAGCCTCCACCACCCCGAGCAGGAAGCGGATGCCGATCAGCAT > NC_002947/3824607‑3824839 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |