Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 3,827,925 G→A 100% S29F (TCC→TTC)  ptxS ← 2‑ketogluconate utilization repressor

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029473,827,9250GA96.4% 32.9 / ‑6.6 12S29F (TCC→TTC) ptxS2‑ketogluconate utilization repressor
Reads supporting (aligned to +/- strand):  ref base G (0/0);  major base A (5/6);  minor base T (0/1);  total (5/7)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

ATCAGTCGCGTCTGGCCGCGCTTTAGGCCGCGAGCCATCTGGTTGGGGCGATAGCCGAGGCGTTCGATGACCTCTTCCAGCCGCTTGGCCGTGGCTTCGGCCAGCAATTGGCGGTCGCCACCGATGTAACGGGAGACGGTGGCTTTGGAAACGCCAGCAACACGCGCCACTTCACTGATGGTGACCCGTTCGCGGGTATGGGCAGGCGCGTCGGTCATGGGTTTTCCTTATGATTGTTTTAATAGTGATGAAA  >  NC_002947/3827793‑3828045
                                                                                                                                    |                                                                                                                        
aTCAGTCGCGTCTGGCCGCGCTTTAGGCCGCGAGCCATCTGGTTGGGGCGATAGCCGAGGCGTTCGATGACCTCTTCCAGCCGCTTGGCCGTGGCTTCGGCCAGCAATTGGCGGTCGCCACCGATGTAACGGaaga                                                                                                                       <  2:401510/136‑1 (MQ=255)
            tGGCCGCGCTTTAGGCCGCGAGCCATCTGGTTGGGGCGATAGCCGAGGCGTTCGATGACCTCTTCCAGCCGCTTGGCCGTGGCTTCGGCCAGCAATTGGCGGTCGCCACCGATGTAACGGAAGACGGTGGCTTTgg                                                                                                           <  2:91608/136‑1 (MQ=255)
                       tAGGCCGCGAGCCATCTGGTTGGGGCGATAGCCGAGGCGTTCGATGACCTCTTCCAGCCGCTTGGCCGTGGCTTCGGCCAGCAATTGGCGGTCACCACCGATGTAACGGTAGACGGTGGCTTTGGAAACGCCAGCa                                                                                                <  1:330875/136‑1 (MQ=255)
                                 gCCATCTGGTTGGGGCGATAGCCGAGGCGTTCGATGCCCTCTTCCAGCCGCTTGGCCGTGGCTTCGGCCAGCAATTGGCGGTCGCCACCGATGTAACGGAAGACGGTGGCTTTGGAAACGCCAGCAACACGCGCCa                                                                                      >  1:132418/1‑136 (MQ=255)
                                   cATCTGGTTGGGGCGATAGCCGAGGCGTTCGATGACCTCTTCCAGCCGCTTGGCCGTGGCTTCGGCCAGCAATTGGCGGTCGCCACCGATGTAACGGAAGACGGTGGCTTTGGAAACGCCAGCAACACGCGCCACt                                                                                    <  1:19724/136‑1 (MQ=255)
                                              ggCGATAGCCGAGGCGTTCGATGACCTCTTCCAGCCGCTTGGCCGTGGCTTCGGCCAGCAATTGGCGGTCGCCACCGATGTAACGGAAGACGGTGGCTTTGGAAACGCCAGCAACACGCGCCACTTCACTGATGGt                                                                         <  2:384667/136‑1 (MQ=255)
                                                                       ctctTCCAGCCGCTTGGCCGTGGCTTCGGCCAGCAATTGGCGGTCGCCACCGATGTAACGGAAGACGGTGGCTTTGGAAACGCCAGCAACACGCGCCACTTCACTGATGGTGACCCGTTCGCGGGTATGGGCAGgc                                                <  2:283779/136‑1 (MQ=255)
                                                                                       gCCGTGGCTTCGGCCAGCAATTGGCGGTCGCCACTGATGTAACGGAAGACGGTGGCTTTGGAAACGCCAGCAACACGCGCCACTTCACTGATGGTGACCCGTTCGCGGGTATGGGCAGGCGCGTCGGTCATGGGtg                                >  1:60273/1‑135 (MQ=255)
                                                                                              cTTCGGCCAGCAATTGGCGGTCGCCACCGATGTAACGGAAGACGGTGGCTTTGGAAACGCCAGCAACACGCGCCACTTCACTGATGGTGACCCGTTCGC‑GGTATGGGCAGGCGCGTCGGTCATGGGTTTTCCTTAt                        <  2:37506/136‑1 (MQ=255)
                                                                                               ttCGGCCAGCAATTGGCGGTCGCCACCGATGTAACGGAAGACGGTGGCTTTGGAAACGCCAGCAACACGCGCCACTTCACTGATGGTGACCCGTTCGCGGGTATGGGCAGGCGCGTCGGTCATGGGTTTTCCTTAt                        <  2:355822/136‑1 (MQ=255)
                                                                                                     cAGCAATTGGCGGTCGCCACCGATGTAACGGAAGACGGTGGCTTTGGAAACGCCAGCAACACGCGCCACTTCACTGATGGTGACCCGTTCGCGGGTATGGGCAGGCGCGTCGGTCATGGGTTTTCCTTATGATTgg                  >  1:453383/1‑135 (MQ=255)
                                                                                                                     ccACCGATGTAACGGAAGACGGTGGCTTTGGAAACGCCAGCAACACGCGCCACTTCACTGATGGTGACCCGTTCGCGGGTATGGGCAGGCGCGTCGGTCATGGGTTTTCCTTATGATTGTTTTAATAGTGATGaaa  >  1:470647/1‑136 (MQ=255)
                                                                                                                     ccACCGATGTAACGGAAGACGGTGGCTTTGGAAACGCCAGCAACACGCGCCACTTCACTGATGGTGACCCGTTCGCGGGTATGGGCAGGCGCGTCGGTCATGGGTTTTCCTTATGATTGTTTTAATAGTGATGaaa  >  2:203164/1‑136 (MQ=255)
                                                                                                                                    |                                                                                                                        
ATCAGTCGCGTCTGGCCGCGCTTTAGGCCGCGAGCCATCTGGTTGGGGCGATAGCCGAGGCGTTCGATGACCTCTTCCAGCCGCTTGGCCGTGGCTTCGGCCAGCAATTGGCGGTCGCCACCGATGTAACGGGAGACGGTGGCTTTGGAAACGCCAGCAACACGCGCCACTTCACTGATGGTGACCCGTTCGCGGGTATGGGCAGGCGCGTCGGTCATGGGTTTTCCTTATGATTGTTTTAATAGTGATGAAA  >  NC_002947/3827793‑3828045

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑