| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 4,665,145 | G→C | 20.6% | G681R (GGC→CGC) | nuoG → | NADH‑quinone oxidoreductase subunit G |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 4,665,145 | 0 | G | C | 20.6% | 43.4 / 3.8 | 19 | G681R (GGC→CGC) | nuoG | NADH‑quinone oxidoreductase subunit G |
| Reads supporting (aligned to +/- strand): ref base G (5/10); new base C (2/2); total (7/12) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.03e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
GCATCCAGATCCACGAAGGCTGGCGCTGGATGCACGCCCTGCGTGCCACCCTGCTGAACAAGCCGGTCGACTGGACCCAGCTGGACCACGTCACCAGCGCCTGCGCCGAAGCCGCTCCGCAACTGGCTGGCATCGTCAACGCCGCGCCGAGCGCTGCGTTCCGCATCAAGGGCATGAAGCTGGCCCGTGAGCCGCTGCGCTACTCCGGCCGTACCGCCATGCGTGCCAACATCAGTGTGCACGAGCCGCG > NC_002947/4665017‑4665266 | gcaTCCAGATCCACGAAGGCTGGCGCTGGATGCACGCCCTGCGTGCCACCCTGCTGAACAAGCCGGTCGACTGGACCCAGCTGGACCACGTCACCAGCGCCTGCGCCGAAGCCGCTCCGCAACTGGCTGGCATCGt > 2:361734/1‑136 (MQ=255)gcaTCCAGATCCACGAAGGCTGGCGCTGGATGCACGCCCTGCGTGCCACCCTGCTGAACAAGCCGGTCGACTGGACCCAGCTGGACCACGTCACCAGCGCCTGCGCCGAAGCCGCTCCGCAACTGGCTGGCATCGt > 2:168595/1‑136 (MQ=255) aTCCAGATCCACGAAGGCTGGCGCTGGATGCACGCCCTGCGTGCCACCCTGCTGAACAAGCCGGTCGACTGGACCCAGCTGGACCACGTCACCAGCGCCTGCGCCGAAGCCGCTCCGCAACTGGCTGGCATCGTCa < 1:361734/136‑1 (MQ=255) aTCCAGATCCACGAAGGCTGGCGCTGGATGCACGCCCTGCGTGCCACCCTGCTGAACAAGCCGGTCGACTGGACCCAGCTGGACCACGTCACCAGCGCCTGCGCCGAAGCCGCTCCGCAACTGGCTGGCATCGTCa < 1:313801/136‑1 (MQ=255) aTCCAGATCCACGAAGGCTGGCGCTGGATGCACGCCCTGCGTGCCACCCTGCTGAACAAGCCGGTCGACTGGACCCAGCTGGACCACGTCACCAGCGCCTGCGCCGAAGCCGCTCCGCAACTGGCTGGCATCGTCa < 1:290778/136‑1 (MQ=255) aTCCAGATCCACGAAGGCTGGCGCTGGATGCACGCCCTGCGTGCCACCCTGCTGAACAAGCCGGTCGACTGGACCCAGCTGGACCACGTCACCAGCGCCTGCGCCGAAGCCGCTCCGCAACTGGCTGGCATCGTCa < 1:280260/136‑1 (MQ=255) aGGCTGGCGCTGGATGCACGCCCTGCGTGCCACCCTGCTGAACAAGCCGGTCGACTGGACCCAGCTGGACCACGTCACCAGCGCCTGCGCCGAAGCCGCTCCGCAACTGGCTGGCATCGTCAACGCCGCGCCGAgc > 1:151325/1‑136 (MQ=255) aTGCACGCCCTGCGTGCCACCCTGCTGAACAAGCCGGTCGACTGGACCCAGCTGGACCACGTCACCAGCGCCTGCGCCGAAGCCGCTCCGCAACTGGCTGGCATCGTCAACGCCGCGCCGAGCGCTGCGTTCCGCa < 2:6807/136‑1 (MQ=255) tGCCACCCTGCTGAACAAGCCGGTCGACTGGACCCAGCTGGACCACGTCACCAGCGCCTGCGCCGAAGCCGCTCCGCAACTGGCTCGCATCGTCAACGCCGCGCCGAGCGCTGCGTTCCGCATCAAGGGCATGAAg > 1:264498/1‑136 (MQ=255) tGCCACCCTGCTGAACAAGCCGGTCGACTGGACCCAGCTGGACCACGTCACCAGCGCCTGCGCCGAAGCCGCTCCGCAACTGGCTCGCATCGTCAACGCCGCGCCGACCGCTGCGTTCCGCATCAAGGGCATGAAg > 1:261973/1‑136 (MQ=255) ctgAACAAGCCGGTCGACTGGACCCAGCTGGACCACGTCACCAGCGCCTGCGCCGAAGCCGCTCCGCAACTGGCTGGCATCGTCAACGCCGCGCCGAGCGCTGCGTTCCGCATCAAGGGCATGAAGCTGGCCCGTg < 2:451592/136‑1 (MQ=255) gAACAAGCCGGTCGACTGGACCCAGCTGGACCACGTCACCAGCGCCTGCGCCGAAGCCGCTCCGCAACTGGCTGGCATCGTCAACGCCGCGCCGAGCGCTGCGTTCCGCATCAAGGGCATGAAGCTGGCCCGTGAg < 1:168595/136‑1 (MQ=255) ggACCCAGCTGGACCACGTCACCAGCGCCTGCGCCGAAGCCGCTCCGCAACTGGCTGGCATCGTCAACGCCGCGCCGAGCGCTGCGTTCCGCATCAAGGGCATGAAGCTGGCCCGTGAGCCGCTGCGCTACTCCgg < 1:397050/136‑1 (MQ=255) tGGACCACGTCACCAGCGCCTGCGCCGAAGCCGCTCCGCAACTGGCTGGCATCGTCAACGCCGCGCCGAGCGCTGCGTTCCGCATCAAGGGCATGAAGCTGGCCCGTGAGCCGCTGCGCTACTCCGGCCGTACCGc > 2:242262/1‑136 (MQ=255) gACCACGTCACCAGCGCCTGCGCCGAAGCCGCTCCGCAACTGGCTGGCATCGTCAACGCCGCGCCGAGCGCTGCGTTCCGCATCAAGGGCATGAAGCTGGCCCGTGAGCCGCTGCGCTACTCCGGCCGTACCGCCa > 1:286652/1‑136 (MQ=255) gACCACGTCACCAGCGCCTGCGCCGAAGCCGCTCCGCAACTGGCTCGCATCGTCAACGCCGCGCCGAGCGCTGCGTTCCGCATCAAGGGCATGAAGCTGGCCCGTGAGCCGCTGCGCTACTCCGGCCGTACCGCCa < 2:261973/136‑1 (MQ=255) gACCACGTCACCAGCGCCTGCGCCGAAGCCGCTCCGCAACTGGCTCGCATCGTCAACGCCGCGCCGAGCGCTGCGTTCCGCATCAAGGGCATGAAGCTGGCCCGTGAGCCGCTGCGCTACTCCGGCCGTACCGCCa < 2:264498/136‑1 (MQ=255) aGCGCCTGCGCCGAAGCCGCTCCGCAACTGGCTGGCATCGTCAACGCCGCGCCGAGCGCTGCGTTCCGCATCAAGGGCATGAAGCTGGCCCGTGAGCCGCTGCGCTACTCCGGCCGTACCGCCATGCGTGCCAACa < 1:139635/136‑1 (MQ=255) cTCCGCAACTGGCTGGCATCGTCAACGCCGCGCCGAGCGCTGCGTTCCGCATAAAGGGCATGAAGCTGGCCCGTGAGCCGCTGCGCTACTCCGGCCGTACCGCCATGCGTGCCAACATCAGTGTGCACGAGCcgcg < 1:27850/136‑1 (MQ=255) | GCATCCAGATCCACGAAGGCTGGCGCTGGATGCACGCCCTGCGTGCCACCCTGCTGAACAAGCCGGTCGACTGGACCCAGCTGGACCACGTCACCAGCGCCTGCGCCGAAGCCGCTCCGCAACTGGCTGGCATCGTCAACGCCGCGCCGAGCGCTGCGTTCCGCATCAAGGGCATGAAGCTGGCCCGTGAGCCGCTGCGCTACTCCGGCCGTACCGCCATGCGTGCCAACATCAGTGTGCACGAGCCGCG > NC_002947/4665017‑4665266 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |