| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 4,748,112 | C→A | 44.4% | V289V (GTG→GTT) | etfA ← | electron transfer flavoprotein subunit alpha |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 4,748,112 | 0 | C | A | 44.4% | 3.5 / 22.1 | 18 | V289V (GTG→GTT) | etfA | electron transfer flavoprotein subunit alpha |
| Reads supporting (aligned to +/- strand): ref base C (4/6); new base A (6/2); total (10/8) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.88e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.41e-01 | |||||||||||
GGGCCGGGTTCTTTATAAGTGAAGCAGGCGGATCAGACCAGCTTTTCCAGCTCCGGAACCGCTTCGAACAGGTCAGCGACCAGGCCGTAGTCGGCCACCTGGAAGATCGGCGCTTCTTCGTCCTTGTTGATCGCCACGATCACTTTGGAGTCTTTCATGCCGGCCAGGTGCTGGATCGCGCCGGAGATACCGACGGCGATGTACAGCTGTGGCGCAACGATCTTGCC > NC_002947/4748017‑4748243 | gggCCGGGTTCTTTATATGTGATGCAGGCGGATCAGACCAGCTTTTCCAGCTCCGGAACCGCTTCGTACAGGTCAGCGACCAGGCCGTAGTCGGCCACCTGGAAGATCGGCGCTTCTTCGTCCTTGTTGATCGCCa < 1:409725/136‑1 (MQ=255) ggCCGGGTTCTTTATAAGTGAAGCAGGCGGATCAGACCAGCTTTTCCAGCTCCGGAACCGCTTCGAACAGGTCAGCGACCAGGCCGTAGTCGGCCACCTGGAAGATCGGCGCTTCTTCGTCCTTGTTGATCGCCAc < 2:12233/136‑1 (MQ=255) cAGGCGGATCAGACCAGCTTTTCCAGCTCCGGAACCGCTTCGAACAGGTCAGCGACCAGGCCGTAGTCGGCCACCTGGAAGATCGGCGCTTCTTCGTCCTTGTTGATCGCCCCGATCACTTTGGAGTCTTTCATGc > 2:407941/1‑136 (MQ=255) cAGGCGGATCAGACCAGCTTTTCCAGCTCCGGAACCGCTTCGAACAGGTCAGCGACCAGGCCGTAGTCGGCCACCTGGAAGATCGGCGCTTCTTCGTCCTTGTTGATCGCCACGATCACTTTGGAGTCTTTCATGc > 2:412680/1‑136 (MQ=255) ggCGGATCAGACCAGGTTTTCCAGCTCCGGAACCGCTTCGAACAGGTCTGCGACCAGGCCGTAGTCGGCCACCTGGAAGATCGGCGCTTCTTCGTCCTTGTAGATCGCCACGATCACTTTGGAGTCTTTCATGCCg < 2:390423/136‑1 (MQ=255) ggCGGATCAGACCAGCTTTTCCAGCTCCGGAACCGCTTCGAACAGGTCAGCGACCAGGCCGTAGTCGGCCACCTGGAAGATCGGCGCTTCTTCGTCCTTGTTGATCGCCACGATCACTTTGGAGTCTTTCATGCCg < 2:227756/136‑1 (MQ=255) ggCGGATCAGACCAGCTTTTCCAGCTCCGGAACCGCTTCGAACAGGTCAGCGACCAGGCCGTAGTCGGCCACCTGGAAGATCGGCGCTTCTTCGTCCTTGTTGATCGCCACGATCACTTTGGAGTCTTTCATGCCg < 2:186486/136‑1 (MQ=255) ttCCAGCTCCGGAACCGCTTCGAACAGGTCAGCGACCAGGCCGTAGTCGGCCACCTGGAAGATCGGCGCTTCTTCGTCCTTGTTGATCGCCACGATCACTTTGGAGTCTTTCATGCCGGCCAGGTGCTGGATcgcg < 2:291647/136‑1 (MQ=255) ggAACCGCTTCGAACAGGTCAGCGACCAGGCCGTAGTCGGCCACCTGGAAGATCGGCGCTTCTTCGTCCTTGTTGATCGCCACGATCACTTTGGAGTCTTTCATGCCGGCCAGGTGCTGGATCGCGCCGGAGATAc > 2:297688/1‑136 (MQ=255) tgAAGAGATCACCGACGAGGCCATAATCGGCAACCTGGAAGATCGGCGCTTCTTCGTCCTTGTTGATGGCGACGATGACGCGTGAGTCCTTCATGCCAGCCAGATGCTGGATCGCGCCGGAAATACCGACAGCGAt > 2:237815/2‑136 (MQ=255) cGAAGAGATCACCGACGAGGCCATAATCGGCAACCTGGAAGATCGGCGCTTCTTCGTCCTTGTTGATGGCGACGATGACGCGTGAGTCCTTCATGCCAGCCAGATGCTGGATCGCGCCGGAAATACCGACAGCGAt < 1:230781/136‑1 (MQ=255) accgacgaGGCCATAATCGGCAACCTGGAAGATCGGCGCTTCTTCGTCCTTGTTGATGGCGACGATGACGCGTGAGTCCTTCATGCCAGCCAGATGCTGGATcg < 2:294148/102‑1 (MQ=255) accgacgaGGCCATAATCGGCAACCTGGAAGATCGGCGCTTCTTCGTCCTTGTTGATGGCGACGATGACGCGTGAGTCCTTCATGCCAGCCAGATGCTGGATcg > 1:294148/3‑104 (MQ=255) gACGAGGCCATAATCGGCAACCTGGAAGATCGGCGCTTCTTCGTCCTTGTTGATGGCGACGATGACGCGTGAGTCCTTCATGCCAGCCAGATGCTGGATCGCGCCGGAAATACCGACAGCGATGTAAAGCtccggt > 1:417825/1‑131 (MQ=255) gACGAGGCCATAATCGGCAACCTGGAAGATCGGCGCTTCTTCGTCCTTGTTGATGGCGACGATGACGCGTGAGTCCTTCATGCCAGCCAGATGCTGGATCGCGCCGGAAATACCGACAGCGATGTAAAGCtccggt > 1:414346/1‑131 (MQ=255) ccGTAGTCGGCCACCTGGAAGATCGGCGCTTCTTCGTCCTTGTTGATCGCCACGATCACTTTGGAGTCTTTCATGCCGGCCAGGTGCTGGATCGCGCCGGAGATACCGACGGCGATGTACAGCTGTGGCGCAACGa > 2:180586/1‑136 (MQ=255) cGGCAACCTGGAAGATCGGCGCTTCTTCGTCCTTGTTGATGGCGACGATGACGCGTGAGTCCTTCATGCCAGCCAGATGCTGGATCGCGCCGGAAATACCGACAGCGATGTAAAGCTCCGGTGCAACCACCTTGcc > 2:318974/1‑136 (MQ=255) cGGCAACCTGGAAGATCGGCGCTTCTTCGTCCTTGTTGATGGCGACGATGACGCGTGAGTCCTTCATGCCAGCCAGATGCTGGATCGCGCCGGAAATACCGACAGCGATGTAAAGCTCCGGTGCAACCACCTTGcc > 1:403618/1‑136 (MQ=255) | GGGCCGGGTTCTTTATAAGTGAAGCAGGCGGATCAGACCAGCTTTTCCAGCTCCGGAACCGCTTCGAACAGGTCAGCGACCAGGCCGTAGTCGGCCACCTGGAAGATCGGCGCTTCTTCGTCCTTGTTGATCGCCACGATCACTTTGGAGTCTTTCATGCCGGCCAGGTGCTGGATCGCGCCGGAGATACCGACGGCGATGTACAGCTGTGGCGCAACGATCTTGCC > NC_002947/4748017‑4748243 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |