Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_002947	4,958,434	(C)5→6	coding (288/1374 nt)	fliI ←	flagellum‑specific ATP synthase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	4,958,429	1	.	C	100.0%	208.0 / NA	55	A98G (GCC→GGC)	fliI	flagellum‑specific ATP synthase
Reads supporting (aligned to +/- strand):  ref base . (0/0);  new base C (24/31);  total (24/31)

CCGTCCATCGGCACCCAGTCCTCGGCCTTCATCCCGCCCTTGCCATCCAGCGCACGGCCGGCGCCGTCGAGCACCCGGCCCAGCATGCTCATGCCCATGGGCAGACGGCCGCCGTCATCCAGCGGCACGACCCGGG‑CCCCCGGCGCGATGCCGACGATACTGCCCACCGGCATCAGAAACACCTTGGGCCCGGCAAAGCCCATGACTTCGGCCTCGACCTGCACCGGGTGATAACTGTCATCGTTGATCACCAGGCAGCGGCTGC  >  NC_002947/4958294‑4958558                                                                                                                                         |                                                                                                                                  ccGTCCATCGGCACCCAGTCCTCGGCCTTCATCCCGCCCTTGCCATCCAGCGCACGGCCGGCGCCGTCGAGCACCCGGCCCAGCATGCTCATGCCCATGGGCAGACGGCCGCCGTCATCCAGCGGCACGACCCggg                                                                                                                                    >  2:861531/1‑136 (MQ=255)     ccATCGGCACCCAGTCCTCGGCCTTCATCCCGCCCTTGCCATCCAGCGCACGGCCGGCGCCGTCGAGCACCCGGCCCAGCATGCTCATGCCCATGGGCAGACGGCCGCCGTCATCCAGCGGCACGACCCGGG‑cccc                                                                                                                               >  1:1323579/1‑136 (MQ=255)     ccATCGGCACCCAGTCCTCGGCCTTCATCCCGCCCTTGCCATCCAGCGCACGGCCGGCGCCGTCGAGCACCCGGCCCAGCATGCTCATGCCCATGGGCAGACGGCCGCCGTCATCCAGCGGCACGACCCGGG‑cccc                                                                                                                               >  2:511241/1‑136 (MQ=255)       aTCGGCACCCAGTCCTCGGCCTTCATCCCGCCCTTGCCATCCAGCGCACGGCCGGCGCCGTCGAGCACCCGGCCCAGCATGCTCATGCCCATGGGCAGACGGCCGCCGTCATCCAGCGGCACGACCCGGG‑cccccc                                                                                                                             >  2:620454/1‑135 (MQ=255)       aTCGGCACCCAGTCCTCGGCCTTCATCCCGCCCTAGCCATCCAGCGCACGGCCGGCGCCGTCGAGCACCCGGCCCAGCATGCTCATGCCCATGGGCAGACGGCCGCCGTCATCCAGCGGCACGACCCGGG‑cccccc                                                                                                                             >  2:938284/1‑135 (MQ=255)       aTCGGCACCCAGTCCTCGGCCTTCATCCCGCCCATGCCATCCAGCGCACGGCCGGCGCCGTCGAGCACCCCGCCCAGCATGCTCATGCCCATGGGCAGACGGCCGCCGTCATCCAGCGGCACGACCCGGG‑cccccc                                                                                                                             >  2:1117726/1‑135 (MQ=255)        tCGGCACCCAGTCCCGGGCCTTCATCCCGCCCTTCCCATCCAGCGCCCGGCCGGCGCCGTCGAGCACCCGGCCCAGCATGCTCATGCCCATGGGCAGACGGCCGCCGTCATCCAGCGGCACGACCCGGG‑ccccccg                                                                                                                            <  1:1265315/136‑3 (MQ=255)            cACCCAGTCCTCGGCCTTCAACCCGCCCTTGCCATCCAGCGCACGGCCGGCGCCGTCGAGCACCCGGCCCAGCATGCTCATGCCCATGGGCAGACGGCCGCCGTCATCCAGCGGCACGACCCGGGCCCCCCGgcgc                                                                                                                         <  2:1260268/136‑1 (MQ=255)                 cggccTCGGCCTCCATCCCGCCCTGGCCACCCAGCGCCCGGCCGGCGCCGCCGAGCACCCGGCCCAGCATGCCCATGCCCAGGGGCAGACGGCCGCCGTCATCCAGCGGCACGACCCGGGCCCCCCGGCGCGATGc                                                                                                                    <  2:1121838/133‑1 (MQ=255)                 agaccTCGGCCTTCATCCCGCCCTTGCCATCCAGCGCACGGCCGGCGCCGTCGAGCACCCGGCCCAGCATGCTCATCCCCATGGGCAGACGGCCGCCGTCATCCAGCGGCACGACCCGGGCCCCCCGGCGCGATGc                                                                                                                    <  2:905175/133‑1 (MQ=255)                 aGTCCTCGGCCTTCATCCCGCCCTTGCCATCCAGCGCACGGCCGGCGCCGTCGAGCACCCGGCCCAGCATGCTCATGCCCATGGGCAGACGGCCGCCGTCATCCAGCGGCACGACCCGGGCCCCCCGGCGCGATGc                                                                                                                    <  2:1166220/136‑1 (MQ=255)                 aGTCCTCGGCCTTCATCCCGCCCTTGCCATCCAGCGCACGGCCGGCGCCGTCGAGCACCCGGCCCAGCATGCTCATGCCCATGGGCAGACGGCCGCCGTCATCCAGCGGCACGACCCGGGCCCCCCGGCGCGATGc                                                                                                                    <  2:234210/136‑1 (MQ=255)                 aGTCCTCGGCCTTCATCCCGCCCTTGCCATCCAGCGCACGGCCGGCGCCGTCGAGCACCCGGCCCAGCATGCTCATGCCCATGGGCAGACGGCCGCCGTCATCCAGCGGCACGACCCGGGCCCCCCGGCGCGATGc                                                                                                                    >  2:47647/1‑136 (MQ=255)                      tCGGCCTTCATCCCGCCCTTGCCATCCAGCGCACGGCCGGCGCCGTCGAGCACCCGGCCCAGCATGCTCATGCCCATGGGCAGACGGCCGCCGTCATCCAGCGGCACGACCCGGGCCCCCCGGCGCGATGCcgacg                                                                                                               <  1:531539/136‑1 (MQ=255)                                tCCCGCCCTTGCCATCCAGCGTTGGGCGGGGGCCGTGGATCAGCCGGCCCAGCATGCTAATGCCCATGGGAAGACGGCCGCCGTCATCCTGCGGCACGACCCGGGCCCCCCGGCGCGATGCCGACGATACTGCCCa                                                                                                     <  2:547979/136‑1 (MQ=255)                                    gCCCTTGCCATCCAGCGCACGGCCGGCGCCGTCGAGCACCCGGCCCAGCATGCTCATGCCCATGGGCAGACGGCCGCCGTCATCCAGCGGCACGACCCGGGCCCCCCGGCGCGATGCCGACGATACTGCCCACCgg                                                                                                 <  2:1300146/136‑1 (MQ=255)                                       cTTGCCATCCAGCGCACGGCCGGCGCCGTCGAGCACCCGGCCCAGCATGCTCATGCCCATGGGCAGCCGGCCGCCGTCATCCAGCGGCACGACCCGGGCCCCCCGGCGCGATGCCGACGATACTGCCCACCGGCAt                                                                                              >  1:1009135/1‑136 (MQ=255)                                       cTTGCCATCCAGCGCACGGCCGGCGCCGTCGAGCACCCGGCCCAGCATGCTCATGCCCATGGGCAGACGGCCGCCGTCATCCAGCGGCACGACCCGGGCCCCCCGCCGCGATGCCGACGATACTGCCCACCGGCAt                                                                                              >  1:1355820/1‑136 (MQ=255)                                        ttGCCATCCAGCGCACGGCCGGCGCCGTCGAGCACCCGGCCCAGCATGCTCATGCCCATGGGCAGACGGCCGCCGTCATCCAGCGGCACGACCCGGGCCCCCCGGCGCGATGCCGACGATACTGCCCACCGGCATc                                                                                             <  1:228556/136‑1 (MQ=255)                                          gCCATCCAGCGCACGGCCGGCGCCGTCGAGCACCCGGCCCAGCATGCTCATGCCCATGGGCAGACGGCCGCCGTCATCCAGCGGCACGACCCGGGCCCCCCGGCGCGATGCCGACGATACTGCCCACCGGCATCAg                                                                                           <  1:868390/136‑1 (MQ=255)                                                  gcgcACGGCCGGCGCCGTCGAGCACCCGGCCCAGCATGCTCATGCCCATGGGCAGACGGCCGCCGTCATCCAGCGGCACGACCCGGGCCCCCCGGCGCGATGCCGACGATACTGCCCACCGGCATCAGAAACACCt                                                                                   >  1:842341/1‑136 (MQ=255)                                                     cACGGCCGGCGCCGTCGAGCACCCGGCCCAGCATGCTCATGCCCATGGGCAGACGGCCGCCGTCATCCAGCGGCACGACCCGGGCCCCCCGGCGCGAAGCCGACGAAACTGCCCACCGGCAACAGCAACACCTTgg                                                                                >  1:167959/1‑136 (MQ=255)                                                      aCGGCCGGCGCCGTCGAGCACCCGGCCCAGCATGCTCATGCCCATGGGCAGACGGCCGCCGTCATCCAGCGGCACGACCCGGGCCCCCCGGCGCGATGCCGACGATACTGCCCACCGGCATCAGAAACACCTTggg                                                                               >  1:1056832/1‑136 (MQ=255)                                                          ccggcgccggcgAGCACCCGGCCCAGCATGCTCATGCCCATGGGCAGACGGCCGCCGGCATCCAGCGGCACGACCCGGGCCCCCCGGCGCGATGCCGACGATACTGCCCACCGGCATCAGAAACACCTTGGGCCCg                                                                           <  1:952698/136‑1 (MQ=255)                                                          ccggcGCCGTCGAGCACCCGGCCCAGCATGCTCATGCCCATGGGCAGACGGCCGCCGTCATCCAGCGGCACGACCCGGGCCCCCCGGCGCGATGCCGACGATACTGCCCACCGGCATCAGAAACACCTTGGGCCCg                                                                           <  1:834836/136‑1 (MQ=255)                                                           cggcGCCGTCGAGCACCCGGCCCAGCATGCTCATGCCCATGGGCAGACGGCCGCCGTCATCCAGCGGCACGACCCGGGCCCCCCGGCGCGATGCCGACGATACTGCCCACCGGCATCAGAAACACCTTGGGCCCgg                                                                          <  2:1323579/136‑1 (MQ=255)                                                            ggcGCCGTCGAGCACCCGGCCCAGCATGCTCATGCCCATGGGCAGACGGCCGCCGTCATCCAGCGGCACGACCCGGGCCCCCCGGCGCGATGCCGACGATACTGCCCACCGGCATCa                                                                                            <  1:1043335/117‑1 (MQ=255)                                                            ggcGCCGTCGAGCACCCGGCCCAGCATGCTCATGCCCATGGGCAGACGGCCGCCGTCATCCAGCGGCACGACCCGGGCCCCCCGGCGCGATGCCGACGATACTGCCCACCGGCATCa                                                                                            >  2:1043335/1‑117 (MQ=255)                                                                 cGTCGAGCACCCGGCCCAGCATGCTCATGCCCATGGGCAGACGGCCGCCGTCATCCAGCGGCACGACCCGGGCCCCCCGGCGCGATGCCGACGATACTGCCCACCGGCATCAGAAACACCTTGGGCCCGGCAAAGc                                                                    <  2:89492/136‑1 (MQ=255)                                                                  gTCGAGCACCCGGCCCTGCATGCTCATGCCCTTGGGCAGACGGCCGCCGTCATCCAGCGGCACGACCCGGGCCCCCCGGCGCGATGCCGACGATACTGCCCACCGGCATCAGAAACACCTTGGGCCAGGCAAAGcc                                                                   <  1:973733/136‑1 (MQ=255)                                                                      aGCACCCGGCCCAGCATGCTCATGCCCATGGGCAGACGGCCGCCGTCATCCAGCGGCACGACCCGGGCCCCCCGGCGCGATGCCGACGATACTGCCCACCGGCATCAGAAACACCTTGGGCCCGGCAAAGCCCATg                                                               >  2:46616/1‑136 (MQ=255)                                                                           ccGGCCCAGCATGCTCATGCCCATGGGCAGACGGCCGCCGTCATCCAGCGGCACGACCCGGGCCCCCCGGCGCGATGCCGACGATACTGCCCACCGGCATCAGAAACACCTTGGGCCCGGCAAAGCCCATGACTTc                                                          >  1:557038/1‑136 (MQ=255)                                                                            cGGCCCAGCATGCTCATGCCCATGGGCAGACGGCCGCCGTCATCCAGCGGCACGACCCGGGCCCCCCGGCGCGATGCCGACGATACTGCCCACCGGCATCAGAAACACCTTGGGCCCGGCAAAGCCCATGACTTCg                                                         <  1:907835/136‑1 (MQ=255)                                                                            cGGCCCAGCATGCTCATGCCCATGGGCAGACGGCCGCCGTCATCCAGCGGCACGACCCGGGCCCCCCGGCGCGATGCCGACGATACTGCCCACCGGCATCAGAAACACCTTGGGCCCGGCAAAGCCCATGACTTCg                                                         <  1:328864/136‑1 (MQ=255)                                                                                ccAGCATGCTCATGCCCATGGGCAGACGGCCGCCGTCATCCAGCGGCACGACCCGGGCCCCCCGGCGCGATGCCGCCGATACTGCCCACCGGCATCAGAAACACCTTGGGCCCGGCAAAGCCCATGACTTCGGCCt                                                     >  2:836154/1‑136 (MQ=255)                                                                                ccAGCATGCTCATGCCCATGGGCAGACGGCCGCCGTCATCCAGCGGCACGACCCGGGCCCCCCGGCGCGATGCCGACGATACTGCCCACCGGCATCAGAAACACCTTGGGCCCGGCAAAGCCCATGACTTCGGcca                                                     >  1:363894/1‑135 (MQ=255)                                                                                ccAGCATGCTCATGCCCATGGGCAGACGGCCGCCGTCATCCAGCGGCACGACCCGGGCCCCCCGGCGCGATGCCGACGATACTGCCCACCGGCATCAGAAACACCTTGGGCCCGGCAAAGCCCATGACTTCGGCCt                                                     >  2:638188/1‑136 (MQ=255)                                                                                ccAGCATGCTCATGCCCATGGGCAGACGGCCGCCGTCATCCAGCGGCACGACCCGGGCCCCCCGGCGCGATGCCGACGATACTGCCCACCGGCATCAGAAACACCTTGGGCCCGGCAAAGCCCATGACTTCGGCCt                                                     <  2:1353459/136‑1 (MQ=255)                                                                                       gCTCATGCCCTTGGGCAGACGGCCGCCGTCATCCAGCGGCACGACCCGGGCCCCCCGGCGCGATGCCGACGATACTGCCCACCGGCATCAGAAACACCTTGGGCCCGGCAAAGCCCATGACTTCGGCCTCGACCTg                                              <  2:1275092/136‑1 (MQ=255)                                                                                       gCTCATGCCCATGGGCAGACGGCCGCCGTCATCCAGCGGCACGACCCGGGCCCCCCGGCGCGATGCCGACGATACTGCCCACCGGCATCAGAAACACCTTGGGCCCGGCAAAGCCCATGACTTCGGCCTCGACCTg                                              <  1:45468/136‑1 (MQ=255)                                                                                         tcttGCCCTTGGTCAGAAGGCCGCCGTCATCCAGGTTCACGACGCGGGCCCCCCGGCGCGATGCCGACGATACTGCCCACCGTCATCAGAATCACCTTGGGCCCGGCAAAGCCCATGACTTCGGCCTCGACCTGCa                                            <  1:891152/133‑1 (MQ=255)                                                                                         tCATGCCCATGGGCTGACGGCCGCCGTCATCCAGCGGCACGACCCGGGCCCCCCGGCGCGATGCCGACGATACTGCCCACCGGCATCAGAAACACCTTGGGCCCGGCAAAGCCCATGACTTCGGCCTCGACCTGCa                                            <  2:1103409/136‑1 (MQ=255)                                                                                         ccATGCCCATGGGCAGACGGCCGCCGTCATCCAGCGGCACGACCCGGGCCCCCCGGCGCGATGCCGACGATACTGCCCACCGGCATCAGAAACACCTTGGGCCCGGCAAAGCCCATGACTTCGGCCTCGACCTGCa                                            <  2:301463/135‑1 (MQ=255)                                                                                            tGCCCATGGGCAGACGGCCGCCGTCATCCAGCGGCACGACCCGGGCCCCCCGGCGCGATGCCGACGATACTGCCCACCGGCATCAGAAACACCTTGGGCCCGGCAAAGCCCATGACTTCGGCCTCGACCTGCACCg                                         >  1:527891/1‑136 (MQ=255)                                                                                              cccATGGGCAGACGGCCGCCGTCATCCAGCGGCACGACCCGGGCCCCCCGGCGCGATGCCGACGATACTGCCCACCGGCATCAGAAACACCTTCGGCCCGGCAAAGCCCATGACTTCGGCCTCGACCAGCAccccg                                       >  2:1072265/1‑133 (MQ=255)                                                                                               ccATGGGCAGACGGCCGCCGTCATCCAGCGGCACGACCCGGGCCCCCCGGCGCGATGCCGACGATACTGCCCACCGGCATCAGAAACACCTTGGGCCCGGCAAAGCCCATGACTTCGGCCTCGACCTGCACCGGGt                                      <  1:47647/136‑1 (MQ=255)                                                                                               ccATGGGCAGACGGCCGCCGTCATCCAGCGGCACGACCCGGGCCCCCCGGCGCGATGCCGACGATACTGCCCACCGGCATCAGAAACACCTTGGGCCCGGCAAAGCCCATGACTTCGGCCTCGACCTGCACCGGGt                                      <  1:46616/136‑1 (MQ=255)                                                                                                cATGGGCAGACGGCCGCCGTCATCCAGCGGCACGACCCGGGCCCCCCGGCGCGATGCCGACGATACTGCCCACCCGCATCAGAAACACCTTGGGCCCGGCAAAGCCCATGACTTCGGCCTCGCCCTGCACCGGGTg                                     >  1:254994/1‑136 (MQ=255)                                                                                                     gCAGACGGCCGCCGTCATCCAGCGGCACGACCCGGGCCCCCCGGCGCGATGCCGACGATACTGCCCACCGGCATCAGAAACACCTTGGGCCCGGCAAAGCCCATGACTTCGGCCTCGACCTGCACCGGGTGATAAc                                >  1:539118/1‑136 (MQ=255)                                                                                                      cAGACGGCCGCCGTCATCCAGCGGCACGACCCGGGCCCCCCGGCGCGATGCCGACGATACTGCCCACCGGCATCAGAAACACCTTGGGCCCGGCAAAGCCCATGACTTCGGCCTCGACCTGCACCGGGTGATAACt                               >  2:362823/1‑136 (MQ=255)                                                                                                      cAGACGGCCGCCGTCATCCAGCGGCACGACCCGGGCCCCCCGGCGCGATGCCGACGATACTGCCCACCGGCATCAGAAACACCTTGGGCCCGGCAAAGCCCATGACTTCGGCCTCGACCTGCACCGGGTGATAACt                               >  2:821185/1‑136 (MQ=255)                                                                                                            gccgccGTCATCCAGCGGCACGACCCGGGCCCCCCGGCGCGATGCCGACGATACTGCCCACCGGCATCAGAAACACCTTGGGCCCGGCAAAGCCCATGACTTCGGCCTCGACCTGCACCGGGTGATAACTGTCATc                         >  1:756377/1‑136 (MQ=255)                                                                                                            gccgccGTCATCCACCGGCACGACCCGGGCCCCCCGGCGCGATGCCGACGATACTGCCCACCGGCATCAGAAACACCTTGGGCCCGGCAAAGCCCATGACTTCGGCCTCGACCTGCACCGGGTGATAACTGTCATc                         <  1:818063/136‑1 (MQ=255)                                                                                                                       ccAGCGGCACGACCCGGGCCCCCCGGCGCGATGCCGACGATACTGCCCACCGGCATCAGAAACACCTTGGGCCCGGCAAAGCCCATGACTTCGGCCTCGACCTGCACCGGGTGATAACTGTCATCGTTGATCACCa              <  1:938284/136‑1 (MQ=255)                                                                                                                        cAGCGGCACGACCCGGGCCCCCCGGCGCGATGCCGACGATACTGCCCACCGGCATCAGAAACACCTTGGGCCCGGCAAAGCCCATGACTTCGGCCTCGACCTGCACCGGGTGATAACTGTCATCGTTGATCACCAg             >  1:1109034/1‑136 (MQ=255)                                                                                                                         aGCGGCACGACCCGGGCCCCCCGGCGCGATGCCGACGATACTGCCCACCGGCATCAGAAACACCTTGGGCCCGGCAAAGCCCATGACTTCGGCCTCGACCTGCACCGGGTGATAACTGTCATCGTTGATCACCAgg            <  1:162186/136‑1 (MQ=255)                                                                                                                            ggCACGACCCGGGCCCCCCGGCGCGATGCCGACGATACTGCCCACCGGCATCAGAAACACCTTGGGCCCGGCAAAGCCCATGACTTCGGCCTCGACCTGCACCGGGTGATAACTGTCATCGTTGATCACCAGGCAg         <  2:842341/136‑1 (MQ=255)                                                                                                                              cACGACCCGGGCCCCCCGGCGCGATGCCGACGATACTGCCCACCGGCATCAGAAACACCTTGGGCCCGGCAAAGCCCATGACTTCGGCCTCGACCTGCACCGGGTGATAACTGTCATCGTTGATCACCAGGCAGCg       >  2:524112/1‑136 (MQ=255)                                                                                                                              cACGACCCGGGCCCCCCGGCGCGATGCCGACGATACTGCCCACCGGCATCAGAAACACCTAGGGCCCGGCAAAGCCCATGACTTCGGCCTCGCCCTGCACCGGGAGATAACAGTCATCGTTGATCACCAGGCAGCg       >  1:498525/1‑136 (MQ=255)                                                                                                                                cGACCCGGGCCCCCCGGCGCGATGCCGACGATACTGCCCACCGGCATCAGAAACACCTTGGGCCCGGCAAAGCCCATGACTTCGGCCTCGACCTGCACCGGGTGATAACTGTCATCGTTGATCACCAGGCAGCGGc     >  2:64489/1‑136 (MQ=255)                                                                                                                                 gACCCGGGCCCCCCGGCGCGATGCCGACGATACTGCCCACCGGCATCAGAAACACCTTGGGCCCGGCAAAGCCCATGACTTCGGCCTCGACCTGCACCGGGTGATAACTGTCATCGTTGATCACCAGGCAGCGGCt    <  1:325119/136‑1 (MQ=255)                                                                                                                                   cccGGGCCCCCCGGCGCGATGCCGACGATACTGCCCACCGGCATCAGAAACACCTTGGGCCCGGCAAAGCCCATGACTTCGGCCTCGACCTGCACCGGGTGATAACTGTCATCGTTGATCACCAGGCAGCGGCTGc  >  1:968537/1‑136 (MQ=255)                                                                                                                                         |                                                                                                                                  CCGTCCATCGGCACCCAGTCCTCGGCCTTCATCCCGCCCTTGCCATCCAGCGCACGGCCGGCGCCGTCGAGCACCCGGCCCAGCATGCTCATGCCCATGGGCAGACGGCCGCCGTCATCCAGCGGCACGACCCGGG‑CCCCCGGCGCGATGCCGACGATACTGCCCACCGGCATCAGAAACACCTTGGGCCCGGCAAAGCCCATGACTTCGGCCTCGACCTGCACCGGGTGATAACTGTCATCGTTGATCACCAGGCAGCGGCTGC  >  NC_002947/4958294‑4958558

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑