Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_002947	5,641,230	(C)7→8	coding (349/1320 nt)	PP_4950 →	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	5,641,223	1	.	C	90.9%	186.0 / 11.2	55	coding (342/1320 nt)	PP_4950	hypothetical protein
Reads supporting (aligned to +/- strand):  ref base . (3/2);  new base C (20/30);  total (23/32)
Fisher's exact test for biased strand distribution p-value = 6.39e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.86e-01

GGCGACGTGCAACTGGATCGCCAGCGCCTGAACGTTGCGCTCGAGCAGCTGCGCCAGACCTTGCCGCTGCTGGCGCTCGACCCTTACCTGCGCCTGGACGAAAACGCCTGGCACAGCCATAGCCTGCAGGA‑A‑‑CCCCCC‑CTGCCCGCACTGAACGAAGTACTGGCCCTGCTCGAGCGTGAAGCAGGTGACCTGGACCTGGTCGGCATCTACGCCGCCGGCCCGGTATGCCGCGGCTTCGCCAGCTCGTTCGGCGCCTTCGGCTGG  >  NC_002947/5641092‑5641355                                                                                                                                      |                                                                                                                                       ggcgACGTGCAACTGGATCGCCAGCGCCTGAACGTTGCGCTCGAGCAGCTGCGCCAGACCTTGCCGCTGCTGGCGCTCGACCCTTACCTGCGCCTGGACGAAAACGCCTGGCACAGCCATAGCCTGCAGGA‑A‑‑cccc                                                                                                                                   >  1:661231/1‑136 (MQ=255)     aCGTGCAACTGGATCGCCAGCGCCTGAACGTTGCGCTCGAGCAGCTGCGCCAGACCTTGCCGCTGCTGGCGCTCGACCCTTACCTGCGCCTGGACGAAAACGCCTGGCACAGCCATAGCCTGCAGGA‑A‑‑cccccc‑cc                                                                                                                              <  1:1093265/136‑2 (MQ=255)     aCGTGCAACTGGATCGCCAGCGCCTGAACGTTGCGCTCGAGCAGCTGCGCCAGACCTTGCCGCTGCTGGCGCTCGACCCTTACCTGCGCCTGGACGAAAACGCCTGGCACAGCCATAGCCTGCAGGA‑A‑‑cccccc‑cc                                                                                                                              >  1:570568/1‑135 (MQ=255)     aCGTGCAACTGGATCGCCAGCGCCTGAACGTTGCGCTCGAGCAGCTGCGCCAGACCTTGCCGCTGCTGGCGCTCGACCCTTACCTGCGCCTGGACGAAAACGCCTGGCACAGCCATAGCCTGCAGGA‑A‑‑cccccc‑cc                                                                                                                              >  1:646089/1‑135 (MQ=255)          cAACTGGATCGCCAGCGCCTGAACGTTGCGCTCGAGCAGCTGCGCCAGACCTTGCCGCTGCTGGCGCTCGACCCTTACCTGCGCCTGGACGAAAACGCCTGGCACAGCCATAGCCTGCAGGA‑AC‑CCCCCC‑CTGccc                                                                                                                          <  1:485110/136‑1 (MQ=255)           aaCTGGATCGCCAGCGCCTGAACGTTGCGCTCGAGCAGCTGCGCCAGACCTTGCCGCTGCTGGCGCTCGACCCTTACCTGCGCCTGGACGAAAACGCCTGGCACAGCCATAGCCTGCAGGA‑AC‑CCCCCC‑CTGCCCg                                                                                                                         >  2:1176043/1‑136 (MQ=255)           aaCTGGATCGCCAGCGCCTGAACGTTGCGCTCGAGCAGCTGCGCCAGACCTTGCCGCTGCTGGCGCTCGACCCTTACCTGCGCCTGGACGAAAACGCCTGGCACAGCCATAGCCTGCAGGA‑AC‑CCCCCC‑CTGCCCg                                                                                                                         >  1:721341/1‑136 (MQ=255)               ggATCGCCAGCGCCTGAACGTTGCGCTCGAGCAGCTGCGCCAGACCTTGCCGCTGCTGGCGCTCGACCCTTACCTGCGCCTGGACGAAAACGCCTGGCACAGCCATAGCCTGCAGGA‑AC‑CCCCCC‑CTGCCCGCACt                                                                                                                     <  2:721341/136‑1 (MQ=255)                gATCGCCAGCGCCTGAACGTTGCGCTCGAGCAGCTGCGTCAGACCTTGCCGCTGCTGGCGCTCGACCCTTACCTGCGCCTGGACGAAAACGCCTGGCACAGCCATAGCCTGCAGGA‑A‑‑CCCCCC‑CTGCCCGCACTGa                                                                                                                   <  2:541290/136‑1 (MQ=255)                gATCGCCAGCGCCTGAACGTTGCGCTCGAGCAGCTGCGCCAGACCTTGCCGCTGCTGGCGCTCGACCCATACCTGCGCCTGGACGAAAACGCCTGGCACAGCCATAGCCTGCAGGA‑AC‑CCCCCC‑CTGCCCGCACTg                                                                                                                    <  1:679770/136‑1 (MQ=255)                 aTCGCCAGCGCCTGAACGTTGCGCTCGAGCAGCTGCGCCAGACCTTGCCGCTGCTGGCGCTCGACCCTTACCTGCGCCTGGACGAAAACGCCTGGCACAGCCATAGCCTGCAGGA‑AC‑CCCCCC‑CTGCCCGCACTGa                                                                                                                   <  1:158288/136‑1 (MQ=255)                 aTCGCCAGCGCCTGAACGTTGCGCTCGAGCAGCTGCGCCAGACCTTGCCGCTGCTGGCGCTCGACCCTTACCTGCGCCTGGACGAAAACGCCTGGCACAGCCATAGCCTGCAGGA‑AC‑CCCCCC‑CTGCCCGCACTGa                                                                                                                   <  1:29264/136‑1 (MQ=255)                  tCGCCAGCGCCTGAACGTTGCGCTCGAGCAGCTGCGCCAGACCTTGCCGCTGCTGGCGCTCGACCCTTACCTGCGCCTGGACGAAAACGCCTGGCACAGCCATAGCCTGCAGGA‑AC‑CCCCCC‑CTGCCCGCACTGaa                                                                                                                  <  1:1059732/136‑1 (MQ=255)                   cGCCAGCGCCTGAACGTTGCGCTCGAGCAGCTGCGCCAGACCTTGCCGCTGCTGGCGCTCGACCCTTACCTGCGCCTGGACGAAAACGCCTGGCACAGCCATAGCCTGCAGGA‑AC‑CCCCCC‑CTGCCCGCACTGAAc                                                                                                                 >  1:411308/1‑136 (MQ=255)                      cAGCGCCTGAACGTTGCGCTCGAGCAGCTGCGCCAGACCTTGCCGCTGCTGGCGCTCGACCCTTACCTGCGCCTGGACGAAAACGCCTGGCACAGCCATAGCCTGCAGGA‑AC‑CCCCCC‑CTGCCCGCACTGAACGaa                                                                                                              <  1:1014625/136‑1 (MQ=255)                      cAGCGCCTGAACGTTGCGCTCGAGCAGCTGCGCCAGACCTTGCCGCTGCTGGCGCTCGACCCTTACCTGCGCCTGGACGAAAACGCCTGGCACAGCCATAGCCTGCAGGA‑AC‑CCCCCC‑CTGCCCGCACTGAACGaa                                                                                                              <  1:979542/136‑1 (MQ=255)                      cAGCGCCTGAACGTTGCGCTCGAGCAGCTGCGCCAGACCTTGCCGCTGCTGGCGCTCGACCCTTACCTGCGCCTGGACGAAAACGCCTGGCACAGCCATAGCCTGCAGGA‑AC‑CCCCCC‑CTGCCCGCACTGAACGaa                                                                                                              <  1:1145694/136‑1 (MQ=255)                         cgcCTGAACGTTGCGCTCGAGCAGCTGCGCCAGACCTTGCCGCTGCTGGCGCTCGACCCTTACCTGCGCCTGGACGAAAACGCCTGGCACAGCCATAGCCTGCAGGA‑A‑‑CCCCCC‑CTGCCCGCACTGAACGAAGTAc                                                                                                          <  1:605742/136‑1 (MQ=255)                          gcCTGAACGTTGCGCTCGAGCAGCTGCGCCAGACCTTGCCGCTGCTGGCGCTCGACCCTTACCTGCGCCTGGACGAAAACGCCTGGCACAGCCATAGCCTGCAGGA‑AC‑CCCCCC‑CTGCCCGCACTGAACGAAGTAc                                                                                                          <  1:258673/136‑1 (MQ=255)                          gcCTGAACGTTGCGCTCGAGCAGCTGCGCCAGACCTTGCCGCTGCTGGCGCTCGACCCTTACCTGCGCCTGGACGAAAACGCCTGGCACAGCCATAGCCTGCAGGA‑AC‑CCCCCC‑CTGCCCGCACTGAACGAAGTAc                                                                                                          <  1:510508/136‑1 (MQ=255)                            cTGAACGTTGCGCTCGAGCAGCTGCGCCAGACCTTGCCGCTGCTGGCGCTCGACCCTTACCTGCGCCTGGACGAAAACGCCTGGCACAGCCATAGCCTGCAGGA‑AC‑CCCCCC‑CTGCCCGCACTGAACGAAGTACTg                                                                                                        <  2:863064/136‑1 (MQ=255)                                 cGTTGCGCTCGAGCAGCTGCGCCAGACCTTGCCGCTGCTGGCGCTCGACCCTTACCTGCGCCTGGACGAAAACGCCTGGCACAGCCATAGCCTGCAGGA‑AC‑CCCCCC‑CTGCCCGCACTGAACGAAGTACTGGCCCt                                                                                                   <  1:659297/136‑1 (MQ=255)                                  gTTGCGCTCGAGCAGCTGCGCCAGACCTTGCCGCTGCTGGCGCTCGACCCTTACCTGCGCCTGGACGAAAACGCCTGGCACAGCCATAGCCTGCAGGA‑AC‑CCCCCC‑CTGCCCGCACTGAACGAAGTACTGGCCCTg                                                                                                  <  1:382673/136‑1 (MQ=255)                                  cgtGCGCTCGAGCAGCTGCGCCAGACCTTGCCGCTGCTGGCGCTCGACCCTTACCTGCGCCTGGACGAAAACGCCTGGCACAGCCATAGCCTGCAGGA‑AC‑CCCCCC‑CTGCCCGCACTGAACGAAGTACTGGCCCTg                                                                                                  <  1:218079/134‑1 (MQ=255)                                       gcTCGAGCAGCTGCGCCAGACCTTGCCGCTGCTGGCGCTCGACCCTTACCTGCGCCTGGACGAAAACGCCTGGCACAGCCATAGCCTGCAGGA‑AC‑CCCCCC‑CTGCCCGCACTGAACGAAGTACTGGCCCTGCTCgc                                                                                             >  2:529401/1‑135 (MQ=255)                                       gcTCGAGCAGCTGCGCCAGACCTTGCCGCTGCTGGCGCTCGACCCTTACCTGCGCCTGGACGAAAACGCCTGGCACAGCCATAGCCTGCAGGA‑AC‑CCCCCC‑CTGCCCGCACGGACCGAAGTACTGGCCCTGCTCgg                                                                                             >  2:504423/1‑135 (MQ=255)                                         tCGAGCAGCTGCGCCAGACCTTGCCGCTGCTGGCGCTCGACCCTTACCTGCGCCTGGACGAAAACGCCTGGCACAGCCATAGCCTGCAGGA‑AC‑CCCCCC‑CTGCCCGCACTGAACGAAGTACTGGCCCTGCTCGAGc                                                                                           <  1:705275/136‑1 (MQ=255)                                              cagcTGCGCCAGACCTTGCCGCTGCTGGCGCTCGACCCTTACCTGCGCCTGGACGAAAACGCCTGGCACAGCCATAGCCTGCAGGA‑AC‑CCCCCC‑CTGCCCGCACTGAACGAAGTACTGGCCCTGCTCGAGCGTGaa                                                                                      >  1:650846/1‑136 (MQ=255)                                                       cAGACCTTGCCGCTGCTGGCGCTCGACCCTTACCTGCGCCTGGACGAAAACGCCTGGCACAGCCATAGCCTGCAGGA‑AC‑CCCCCC‑CTGCCCGCACTGAACGAAGTACTGGCCCTGCTCGAGCGTGAAGCAGGTgac                                                                             <  1:335873/136‑1 (MQ=255)                                                          aCCTTGCCGCTGCTGGCGCTCGACCCTTACCTGCGCCTGGACGAAAACGCCTGGCACAGCCATAGCCTGCAGGA‑AC‑CCCCCC‑CTGCCCGCACTGAACGAAGTACTGGCCCTGCTCGAGCGTGAAGCAGGTgacctg                                                                          <  1:630366/136‑1 (MQ=255)                                                          aCCTTGCCGCTGCTGGCGCTCGACCCTTACCTGCGCCTGGACGAAAACGCCTGGCACAGCCATAGCCTGCAGGA‑AC‑CCCCCC‑CTGCCCGCACTGAACGAAGTACTGGCCCTGCTCGAGCGTGAAGCAGGTgacctg                                                                          <  1:1276470/136‑1 (MQ=255)                                                                ccGCTGCTGGCGCTCGACCCTTACCTGCGCCTGGACGAAAACGCCTGGCACAGCCATAGCCTGCAGGA‑AC‑CCCCCC‑CTGCCCGCACTGAACGAAGTACTGGCCCTGCTCGAGCGTGAAGCAGGTgacctggacctg                                                                    <  2:322858/136‑1 (MQ=255)                                                                ccGCTGCTGGCGCTCGACCCTTACCTGCGCCTGGACGAAAACGCCTGGCACAGCCATAGCCTGCAGGA‑AC‑CCCCCC‑CTGCCCGCACTGAACGAAGTACTGGCCCTGCTCGAGCGTGAAGCAGGTgacctggacctg                                                                    <  2:650846/136‑1 (MQ=255)                                                                 cGCTGCTGGCGCTCGACCCTTACCTGCGCCTGGACGAAAACGCCTGGCACAGCCATAGCCTGCAGGA‑ACCCCCCCC‑CTGCCCGCACTGAACGAAGTACTGGCCCTGCGCGAGCGTGAAGCAGGTgacctggacctg                                                                    >  2:60317/1‑136 (MQ=255)                                                                 cGCTGCTGGCGCTCGACCCTTACCTGCGCCTGGACGAAAACGCCTGGCACAGCCATAGCCTGCAGGA‑AC‑CCCCCC‑CTGCCCGCACTGa                                                                                                                   >  2:1353034/1‑88 (MQ=255)                                                                 cGCTGCTGGCGCTCGACCCTTACCTGCGCCTGGACGAAAACGCCTGGCACAGCCATAGCCTGCAGGA‑AC‑CCCCCC‑CTGCCCGCACTGa                                                                                                                   <  1:1353034/88‑1 (MQ=255)                                                                 cGCTGCTGGCGCTCGACCCTTACCTGCGCCTGGACGAAAACGCCTGGCACAGCCATAGCCTGCAGGA‑AC‑CCCCCC‑CTGCCCGCACTGAACGAAGTACTGGCCCTGCTCGAGCGGGAAGCAGGTGacctggacctgg                                                                   >  2:502918/1‑136 (MQ=255)                                                                        ggCGCTCGACCCTTACCTGCGCCTGGACGAAAACGCCTGGCACAGCCATAGCCTGCAGGA‑AC‑CCCCCC‑CTGCCCGCACTGAACGAAGTACTGGCCCTGCTCGAGCGTGAAGCAGGTGACCTGGACCTGGTCGGCAt                                                            <  1:875298/136‑1 (MQ=255)                                                                        ggCGCTCGACCCTTACCTGCGCCTGGACGAAAACGCCTGGCACAGCCATAGCCTGCAGGA‑AC‑CCCCCC‑CTGCCCGCACTGAACGAAGTACTGGCCCTGCTCGAGCGTGAAGCAGGGGACCTGGACCTGGTCGGCAt                                                            <  1:956350/136‑1 (MQ=255)                                                                                   cTTACCTGCGCCTGGACGAAAACGCCTGGCACAGCCATAGCCTGCAGGATC‑‑CCCCCC‑CTGCCCGCACTGAACGAAGTACTGGCCCTGCTCGAGCGTGAAGCAGGTGACCTGGACCTGGTCGGCATCTACGccgccg                                                 >  1:636496/1‑136 (MQ=255)                                                                                         tGCGCCTGGACGAAAACGCCTGGCACAGCCATAGCCTGCAGGA‑AC‑CCCCCC‑CTGCCCGCACTGAACGAAGTACTg                                                                                                        >  2:106975/1‑75 (MQ=255)                                                                                         tGCGCCTGGACGAAAACGCCTGGCACAGCCATAGCCTGCAGGA‑AC‑CCCCCC‑CTGCCCGCACTGAACGAAGTACTg                                                                                                        <  1:106975/75‑1 (MQ=255)                                                                                              cTGGACGAAAACGCCTGGCACAGCCTTAGCCTGCAGGA‑AC‑CCCCCC‑CTGCCCGCACTGAACGAAGTACTGGCCCTGCTCGAGCGTGAAGCAGGTGACCTGGACCTGGTCGGCATCTACGCCGCCGGCCCGGTATGc                                      >  2:844461/1‑136 (MQ=255)                                                                                              cTGGACGAAAACGCCTGGCACAGCCATAGCCTGCAGGA‑AC‑CCCCCC‑CTGCCCGCACTGAACGAAGTTCTGGCCCTGCTCGAGCGTGAAGCAGGTGACCTGGTCCTGGTCGGCATCTACGCCGCCGGCCCGGTATGc                                      >  1:270952/1‑136 (MQ=255)                                                                                              cTGGACGAAAACGCCTGGCACAGCCATAGCCTGCAGGA‑AC‑CCCCCC‑CTGCCCGCACTGAACGAAGTACTGGCCCTGCTCGAGCGTGAAGCAGGTGACCTGGCCCTGGTCGGCCTCTACGCCGCCGGCCCGGTATGc                                      >  1:1047859/1‑136 (MQ=255)                                                                                              cTGGACGAAAACGCCTGGCACAGCCATAGCCTGCAGGA‑AC‑CCCCCC‑CTGCCCGCACTGAACGAAGTACTGGCCCTGCTCGAGCGTGAAGCAGGTGACCTGGACCTGGTCGGCATCTACGCCGCCGGCCCGGTATGc                                      >  2:719371/1‑136 (MQ=255)                                                                                              cTGGAAGAAAACGCCTGGCACAGCCATAGCCTGCAGGA‑AC‑CCCCCC‑CTGCACGCACTGAACGAAGTACTGCCCAAGCACGAGCCAGAACCAGCTGACCAGGACCAGGTCGGCATCAACACCGCCGGCCCCGTATGc                                      >  2:541094/1‑136 (MQ=255)                                                                                                          gCCTGGCACAGCCATAGCCTGCAGGA‑AC‑CCCCCC‑CTGCCCGCACTGAACGAAGTACTGGCCCTGCTCGAGCGTGAAGCAGGTGACCTGGACCTGGTCGGCATCTACGCCGCCGGCCCGGTATGCCGCGGCTTCGcc                          <  2:1145747/136‑1 (MQ=255)                                                                                                                 acaGCCATAGCCTGCAGGA‑AC‑CCCCCC‑CTGCCCGCACTGAACGAAGTACTGGCCCTGCTCGAGCGTGAAGCAGGTGACCTGGACCTGGTCGGCATCTACGCCGCCGGCCCGGTATGCCGCGGCTTCGCCAGCTCGt                   >  1:438175/1‑136 (MQ=255)                                                                                                                       aTAGCCTGCAGGA‑AC‑CCCCCC‑CTGCCCGCACTGAACGAAGTACTGGCCCTGCTCGAGCGTGAAGCAGGTGACCTGGACCTGGTCGGCATCTACGCCGCCGGCCCGGTATGCCGCGGCTTCGCCAGCTCGTTCGgcg             <  2:122512/136‑1 (MQ=255)                                                                                                                       aTAGCCTGCAGGA‑AC‑CCCCCC‑CTGCCCGCACTGAACGAAGTACTGGCCCTGCTCGAGCGTGAAGCAGGTGACCTGGACCTGGTCGGCATCTACGCCGCCGGCCCGGTATGCCGCGGCTTCGCCAGCTCGTTCGgcg             <  2:646089/136‑1 (MQ=255)                                                                                                                       aTAGCCTGCAGGA‑A‑‑CCACCC‑CTGCCCGCACTGAACGAAGTACTGGCCCTGCTAGAGCGTGAAGCAGGTGACCTGGACCTGGTCGGCATCTACGCCGCCTGCCCGGTATGCCGCGGCTTCGCCAGCTCGTTCGgcgc            >  1:871181/1‑136 (MQ=255)                                                                                                                        tAGCCTGCAGGA‑AC‑CCCCCC‑CTGCCCGCACTGAACGAAGTACTGGCCCTGCTCGAGCGTGAAGCAGGTGACCTGGACCTGGTCGGCATCTACGCCGCCGGCCCGGTATGCCGCGGCTTCGCCAGCTCGTTCGgcgc            >  1:1120327/1‑136 (MQ=255)                                                                                                                        tAGCCTGCAGGA‑AC‑CCCCCC‑CTGCCCGCACTGAACGAAGTACTGGCCCTGCTCGAGCGTGAAGCAGGTGACCTGGACCTGGTCGGCATCTACGCCGCCGGCCCGGTATGCCGCGGCTTCGCCAGCTCGTTCGgcgc            >  1:1233747/1‑136 (MQ=255)                                                                                                                        tAGCCTGCAGGA‑AC‑CCCCCC‑CTGCCCGCACTGAACGAAGTACTGGCCCTGCTCGAGCGTGAAGCAGGTGACCTGGACCTGGTCGGCATCTACGCCGCCGGCCCGGTATGCCGCGGCTTCGCCAGCTCGTTCGgcgc            <  2:626985/136‑1 (MQ=255)                                                                                                                            cTGCAGGA‑AC‑CCCCCC‑CTGCCCGCACTGAACGAAGTACTGGCCCTGCTCGAGCGTGAAGCAGGTGACCTGGACCTGGTCGGCATCTACGCCGCCGGCCCGGTATGCCGCGGCTTCGCCAGCTCGTTCGGCGCCTTc        <  2:770970/136‑1 (MQ=255)                                                                                                                             tGCAGGA‑AC‑CCCCCC‑CTGCCCGCACTGAACGAAGTACTGGCCCTGCTCGAGCGTGAAGCAGGTGACCTGGACCTGGTCGGCATCTACGCCGCCGGCCCGGTATGCCGCGGCTTCGCCAGCTCGTTCGGCGCCTTCg       >  2:452745/1‑136 (MQ=255)                                                                                                                                aGGA‑AC‑CCCCCC‑CTGCCCGCACTGAACGAAGTACTGGCCCTGCTCGAGCGTGAAGCAGGTGACCTGGACCTGGTCGGCATCTACGCCGCCGGCCCGGTATGCCGCGGCGTCGCCCGCTCGTTCGGCGCCTTCGGcg    >  1:616132/1‑135 (MQ=255)                                                                                                                                  gA‑A‑‑CCCCCCACTGCCCGCACTGAACGAAGTACTGGCACTGCTCGAGCGTGAAGCAGGTGACCTGGACATGGACGGCAACTACGCCGCCGGCCCGGTATGCCGCGGCATCGCCAGCTCCTTCGGCGCCTACGGCTgg  >  2:146853/1‑136 (MQ=255)                                                                                                                                      |                                                                                                                                       GGCGACGTGCAACTGGATCGCCAGCGCCTGAACGTTGCGCTCGAGCAGCTGCGCCAGACCTTGCCGCTGCTGGCGCTCGACCCTTACCTGCGCCTGGACGAAAACGCCTGGCACAGCCATAGCCTGCAGGA‑A‑‑CCCCCC‑CTGCCCGCACTGAACGAAGTACTGGCCCTGCTCGAGCGTGAAGCAGGTGACCTGGACCTGGTCGGCATCTACGCCGCCGGCCCGGTATGCCGCGGCTTCGCCAGCTCGTTCGGCGCCTTCGGCTGG  >  NC_002947/5641092‑5641355

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑