Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_002947195,6340CT52.5% ‑5.8 / 51.7 40T380T (ACC→ACT) PP_0168putative surface adhesion protein
Reads supporting (aligned to +/- strand):  ref base C (5/14);  new base T (11/10);  total (16/24)
Fisher's exact test for biased strand distribution p-value = 1.17e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: E-value score below prediction cutoff.
Rejected as consensus: Frequency below/above cutoff threshold.

CCAATGACGTCTACAACAACGGTTCGACCGTCAGCGTCACCATCGAGAACGCCACCGGCGGTAATTTCGAGCAACTGACCCCGAATCCGACCCCGGCTCAGACCACGATCAACGACTCGGTCGACACCACCACCGCGACCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTGATCACTTACACCGTGACCCTGAGCAACCCTGCCCAGACGCCGGTAACCGTGAACCTGTCCAACGGCCAAACGATTACCGTTGAAGCGG  >  NC_002947/195504‑195767
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ccAATGACGTCTACAACAACGGTTCGACCGTCAGCGTCACCATCGAGAACGCCACCGGCGGTAATTTCGAGCAACTGACCCCGAATCCGACCCCGGCTCAGACCACGATCAACGACTCGGTCGACACCACCACcgc                                                                                                                                  >  2:939852/1‑136 (MQ=39)
   aTGACGTCTACAACAACGGCTCGACCGTCAGCGTCACCATCGAGAACGCCACCGGCGGTAATTTCGAGCAACTGACCCCGAATCCGACCCCGGCTCAGACCACGATCAACGACTCGGTCGACACCACCACCGCGAc                                                                                                                               <  1:246568/136‑1 (MQ=37)
             aaacaccGGTTGTACCGTAAGCTTCACCTTCTTTAAGGCAACGGGCGTTATTTTCTATCAAATGACCCCGAATGCGACCCGGGCTCTCCCCAGTAACCACGACTCGGTCGACACCACCACCGCGACCCTGACGGCg                                                                                                                     <  2:474320/135‑1 (MQ=2)
                   cGGTTCGACCGTCAGCGTCACCATCGAGAACGCCACAGGCGGTAATTTCGAGCAACTGACCCCGAATCCGACCCCGGCTCAGACCACGATCAACGACTCGGTCGACACCACTACCGCGACCCTGACGGCGAGCCCg                                                                                                               >  1:409071/1‑136 (MQ=17)
                   cGGTTCGACCGTCAGCGTCACCATCGAGAACGCCACAGGCGGTAATTTCGAGCAACTGACCCCGAATCCGACCCCGGCTCAGACCACGATCAACGACTCGGTCGACACCACTACCGCGACCCTGACGGCGAGCCCg                                                                                                               >  1:343800/1‑136 (MQ=17)
                    ggTTCGACCGCCAGCGCCACCATCGACACCGCCACAGGCGGAAATTTCGACCAACTCACCCCGAATCCCACCCCGGCTCACCCCACGATCAACGACTCGGTCGACACCACTACCGCGACCCTGACGGCGAGCCCGt                                                                                                              <  1:986459/136‑1 (MQ=11)
                     gTTCGACCGTCAGCGTCACCATCGAGAACGCCACCGGCGGTAATTTCGAGCAACTGACCCCGAATCCGACCCCGGCTCAGACCACGATCAACGACTCGGTCGACACCACCACCGCGACCCTGACGGCGAGCCCGTc                                                                                                             >  1:46158/1‑136 (MQ=37)
                     gTTCGACCGTCAGCGTCACCATCGAGAACGCCACAGGCGGTAATTTCGAGCAACTGACCCCGAATCCGACCCCGGCTCAGACCACGATCAACGACTCGGTCGACACCACTACCGCGACCCTGACGGCGAGCCCGTc                                                                                                             >  1:713568/1‑136 (MQ=17)
                      ttCGACCGTCAGCGTCACCATCGAGAACGCCACAGGCGGTAATTTCGAGCAACTGACCCCGAATCCGACCCCGGCTCAGACCACGATCAACGACTCGGTCGACACCTCTACCGCGACCCTGCCGGCGAGCCCGTCg                                                                                                            >  1:1119060/1‑136 (MQ=255)
                      ttCGACCGTCAGCGTCACCATCGAGAACGCCACAGGCGGTAATTTCGAGCAACTGACCCCGAATCCGACCCCGGCTCAGACCACGATCAACGACTCGGTCGACACCACTACCGCGACCCTGACGGCGAGCCCGTCg                                                                                                            >  2:684800/1‑136 (MQ=17)
                                   gTCACCATCGAGAACGCCACAGGCGGTAATTTCGAGCAACTGACCCCGAATCCGACCCCGGCTCAGACCACGATCAACGACTCGGTCGACACCACCACCGCGACCCTGACGGCGAGCCCGTCGGTCACCGAAggcg                                                                                               <  1:607406/136‑1 (MQ=17)
                                     cactttCGACACCGCCCCCGGCGTAAATTCCGACCAACTGCCCCCGAATCCCACCCCGGCTCAGACCACGATCAACGACTCGGTCGACACCACCACCGCGACCCTGACGGCGAGCCCGTCGGTCACCGAAggcggc                                                                                             <  1:989235/131‑1 (MQ=11)
                                     cACCATCGAGACCGCCACCGGCGTTAATTTCGACCAACTCCCCCCCAAACCCCCCCCGGCTCACACCACGATCAACGACTCGGTCGACACCACTACCGCGACCCTGACGGCGAGCCCGTCGGTCACCGAAggcggc                                                                                             <  1:1285239/136‑1 (MQ=11)
                                     cACCATCGAGAACGCCACAGGCGGTAATTTCGAGCAACTGACCCCGAATCCGACCCCGGCTCAGACCACGATCAACGACTCGGTCGACACCACTACCGCGACCCTGACGGCGAGCCCGTCGGTCACCGAAggcggc                                                                                             <  1:315889/136‑1 (MQ=17)
                                     cACCATCGAGAACGCCACAGGCGGTAATTTCGAGCAACTGACCCCGAATCCGACCCCGGCTCAGACCACGATCAACGACTCGGTCGACACCACTACCGCGACCCTGACGGCGAGCCCGTCGGTCACCGAAggcggc                                                                                             <  1:401518/136‑1 (MQ=17)
                                     cACCATCGACACCCCCACCGGGGTAAATTTCGAGCAACTGACCCCTAATCCGACCCCGGCTCACACCACGATCAACGACTCGGTCGACACCACCACCGCGACCCTGACGGCGAGCCCGTCGGTCACCGAAggcggc                                                                                             <  1:982376/136‑1 (MQ=11)
                                       ccacccagaACCCCACCGGCGTAAATTTCGACCAACTCCCCCCCAACCCCCCCCCGGCCCCGCCCCCGAACAACGACTCGGTCGACACCACCACCGCGACCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGt                                                                                           <  1:890750/130‑1 (MQ=11)
                                         aTCGAGAACGCCACAGGCGGTAATTTCGAGCAACTGACCCCGAATCCGACCCCGGCTCAGACCACGATCAACGACTCGGTCGACACCACTACCGCGACCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTGa                                                                                         >  1:386559/1‑136 (MQ=17)
                                         aTCGAGAACGCCACAGGCGGTAATTTCGAGCAACTGACCCCGAATCCGACCCCGGCTCAGACCACGATCAACGACTCGGTCGACACCACTACCGCGACCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTGa                                                                                         >  1:354896/1‑136 (MQ=17)
                                           ccccaaCCCCACCGGCGTTAATTTGGCCCCACTGCCCCCGAAACCGCCCCCGGCGCACACCACGATCAACGACTCGGTCGACACCACCACCGCGACCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTGATc                                                                                       <  1:978848/132‑1 (MQ=11)
                                               aaCGCCACAGGCGGTAATTTCGAGCAACTGACCCCGAATCCGACCCCGGCTCAGACCACGATCAACGACTCGGTCGACACCACTACCGCGACCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTGATCACtt                                                                                   >  1:1041348/1‑136 (MQ=17)
                                               aaCGCCACAGGCGGTAATTTCGAGCAACTGACCCCGAATCCGACCCCGGCTCAGACCACGATCAACGACTCGGTCGACACCACTACCGCGACCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTGATCACtt                                                                                   >  2:150799/1‑136 (MQ=17)
                                                  gCCACAGGCGGTAATTTCGAGCAACTGACCCCGAATCCGACCCCGGCTCAGACCACGATCAACGACTCGGTCGACACCACTACCGCGACCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTGATCACTTaca                                                                                <  2:354896/136‑1 (MQ=17)
                                                       aggcggTAATTTCGAGCAACTGACCCCGAATCCGACCCCGGCTCAGACCACGATCAACGACTCGGTCGACACCACTACCGCGACCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTGATCACTTACACCGTg                                                                           <  2:343800/135‑1 (MQ=17)
                                                                     tgCAATTGACCCCGAATCCGACCCCGGCTCAGACCACTATCAACGACTTGGTCTATGCCATCACCGCGACCTTGACGGCGAGCCCGTCGGTCACCGATGGTGGCGTGATCACTTACACCGTGACTCTGAGCAAccc                                                             <  2:159607/135‑1 (MQ=11)
                                                                        aaCTGACCCCGAATCCGACCCCGGCTCAGACCACGATCAACGACTCGGTCGACACCACTACCGCGACCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTGATCACTTACACCGTGACCCTGAGCAACCCTGc                                                          >  1:445151/1‑136 (MQ=21)
                                                                                 ccaaTCGGCCCCCGGCTCACCCCACTATCAACGACTCGGTCGACACCACTACCGCGACCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTGATCACTTACACCGTGACCCTGAGCAACCCTGCCCAGACGcc                                                 <  1:784106/134‑1 (MQ=11)
                                                                                        ccccccCGCTCAAACCCCTCTCAACGACACGTTCGACACAACCACCGCGACCCTGACGGCGAGCCCTTGGGTCACCGAAGGCGGCGTGATCACTTACACCGTGACCCTGAGCAACCCTGCCCAGACGCCGGTAAcc                                          <  1:174961/134‑1 (MQ=11)
                                                                                          ccccGGCTCAGACCACGATCAACGACTCGGTCGACACCACCACCGCGACCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTGATCAc                                                                                     <  1:1282487/91‑1 (MQ=32)
                                                                                          ccccGGCTCAGACCACGATCAACGACTCGGTCGACACCACCACCGCGACCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTGATCAc                                                                                     >  2:1282487/1‑91 (MQ=32)
                                                                                           cccGGCTCAGACCACCATCAACGACTCGGTCGATGCCACCACCGCGACCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTGATCACTTACACCGTGACCCTGAGCAACCCTGCCCAGACGCCGGTGTCCGTg                                       <  1:1262692/136‑1 (MQ=11)
                                                                                                       tgcttacAACGACTCGGGCGACACCACCACCGCGACCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTGATCACTTACACCGTGACCCTGAGCAACCCTGCCCAGACGCCGGTAACCGTGAACCTGTCCAAc                           <  1:568168/130‑1 (MQ=255)
                                                                                                       cACGATCACCGACTCGGTCGACACCACCACCGCGACCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTGATCACTTACACCGTGACCCTGAGCAACCCTGCCCAGACGCCGGTAACCGTGAACCTGTCCAAc                           <  2:1026502/136‑1 (MQ=255)
                                                                                                               acgacgCGGTCCACACCACTACCGCTCCCCTGACGGCGAGCCCTTCGTTCACCGAAGGCGTCGTTATCACTTACCCCCTGACAATTAGCAAAACTGCCAACACGCCGGTAACAGTGAACCTGTCCAACGGCCaaaa                   <  2:1257903/136‑2 (MQ=255)
                                                                                                                  aCTCGGTCGACACCACCACCGCGACCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTGATCACTTACACCGTGACCCTGAGCAACCCTGCCCAGACGCCGGTAACCGTGAACCTGTCCAACGGCCAAACGAt                >  2:1101006/1‑136 (MQ=40)
                                                                                                                       gTCGACACCACTACCGCGACCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTGATCACTTACACCGTGACCCTGAGCAACCCTGCCCAGACGCCGGTAACCGTGAACCTGTCCAACGGCCAAACGATTACCg           >  2:947035/1‑136 (MQ=255)
                                                                                                                         cGACACCTCTCCCGCGACCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTGATCACTTACACCGTGACCCTGAGCAACCCTGCCCAGACGCCGGTAACCGTGAACCTGTCCAACGGCCAAACGATTACCGtt         <  2:1119060/136‑1 (MQ=255)
                                                                                                                         cGACACCACCACCGCGACCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTGATCACTTACACCGTGACCCTGAGCAACCCTGCCCAGACGCCGGTAACCGTGAACCTGTc                               <  2:501224/114‑1 (MQ=255)
                                                                                                                         cGACACCACCACCGCGACCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTGATCACTTACACCGTGACCCTGAGCAACCCTGCCCAGACGCCGGTAACCGTGAACCTGTc                               >  1:501224/1‑114 (MQ=255)
                                                                                                                           atgccaccaccGCGACCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTGATCACTTACACCGTGACCCTGAGCAACCCTGCCCAGACGCCGGTGACCGTGACCCTGTCCAACGGCCAAACGATTACCGTTGa       >  2:20873/4‑136 (MQ=12)
                                                                                                                           acacCACTACCGCGACCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTGATCACTTACACCGTGACCCTGAGCAACCCTGCCCAGACGCCGGTAACCGTGAACCTGTCCAACGGCCAAACGATTACCGTTGa       <  2:1041348/136‑1 (MQ=255)
                                                                                                                              ccaccaccGCGACCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTGATCACTTACACCGTGACCCTGAGCAACCCTGCCCAGACGCCGGTAACCGTGAACCTGTCCAACGGCCAAACGATTACCGTTGAAGc    >  2:880621/1‑136 (MQ=40)
                                                                                                                                accaccGCGCCCCTGCCCGCGAGCCCGGCGGTCCCCGTCGGCGGCGGGAACATTTCCACCCGCACCCGGAGCAACCCTGCCCAGACGCCGGTAACCGTGAACCTGTCCAACGGCCAAACGATTACCGTTGAAGCgg  <  1:815977/136‑1 (MQ=14)
                                                                                                                                accaccGCGACCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTGATCACTTACACCGTGACCCTGAGCAACCCTGCCCAGACGCCGGTAACCGTGAACCTGTCCAACGGCCAAACGATTACCGTTGAAGCgg  <  1:1101006/136‑1 (MQ=40)
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CCAATGACGTCTACAACAACGGTTCGACCGTCAGCGTCACCATCGAGAACGCCACCGGCGGTAATTTCGAGCAACTGACCCCGAATCCGACCCCGGCTCAGACCACGATCAACGACTCGGTCGACACCACCACCGCGACCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTGATCACTTACACCGTGACCCTGAGCAACCCTGCCCAGACGCCGGTAACCGTGAACCTGTCCAACGGCCAAACGATTACCGTTGAAGCGG  >  NC_002947/195504‑195767

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑