| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 923,649 | 0 | T | G | 41.4% | 69.9 / 19.4 | 58 | Q516P (CAG→CCG) | PP_0804 | protein secretion ABC transporter permease/ATP‑binding protein |
| Reads supporting (aligned to +/- strand): ref base T (2/32); new base G (24/0); total (26/32) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.53e-14 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
AGGTGGGCCATGGCGATGCCGTCCAGGCTGATCTCGCCCTGCACCAGATCCATGGCCCCACCCAGCGCCTGCAGCAAGGTTGATTTGCCGGCGCCGTTGCGGCCCAGCACGGCAATGCGCTCGCCCGGCTGGATATCCA‑GCCGGCCAATGTTCAGCACTGGCGGGTAGTCTTCGCTGTAGCGGAAGTTGGCCTGGCGCAGGCGGTACTCGCCGTGAATGGCCGGCAGGTGCACGCGGGCTTCGCCTTCGGGGTGATCGACCGG > NC_002947/923520‑923782 | aGGTGGGCCATGGCGATGCCGTCCAGGCTGATCTCGCCCTGCACCAGATCCATGGCCCCACCCAGCGCCTGCAGCAAGGTTGATTTGCCGGCGCCGTTGCGGCCCAGCACGGCAATGCGCTCGCGCGGCgggggat > 1:97575/1‑132 (MQ=255)aGGTGGGCCATGGCGATGCCGTCCAGGCTGATCTCGCCCTGCACCAGATCCATGGCCCCACCCAGCGCCTGCAGCAAGGTTGATTTGCCGGCGCCGTTGCGGCCCAGCACGGCAATGCGCTCGCGCGGCgggcgtt > 2:496653/1‑132 (MQ=255)aGGTGGGCCATGGCGATGCCGTCCAGGCTGATCTCGCCCTGCACCAGATCCATGGCCCCACCCAGCGCCTGCAGCAAGGTTGATTTGCCGGCGCCGTTGCGGCCCAGCACGGCAATGCGCTCGCGCGGCGGGgtat > 2:1234965/1‑136 (MQ=255)aGGTGGGCCATGGCGATGCCGTCCAGGCTGATCTCGCCCTGCACCAGATCCATGGCCCCACCCAGCGCCTGCAGCAAGGTTGATTTGCCGGCGCCGTTGCGGCCCAGCACGGCAATGCGCTCGCCCGGCGGGagag > 1:524513/1‑133 (MQ=255) ggTGGGCCATGGCGATGCCGTCCAGGCTGATCTCGCCCTGCACCAGATCCATGGCCCCACCCAGCGCCTGCAGCAAGGTTGATTTGCCGGTGCCGTTGCGGCCCAGCACGGCAATGCGCTCGCCCGGCTGGATATc < 1:579073/136‑1 (MQ=255) ggTGGGCCATGGCGATGCCGTCCAGGCTGATCTCGCCCTGCACCAGATCCATGGCCCCACCCAGCGCCTGCAGCAAGGTTGATTTGCCGGCGCCGTTGCGGCCCAGCACGGCAATGCGCTCGCCCGGCTGGATATc < 1:517188/136‑1 (MQ=255) ggTGGGCCATGGCGATGCCGTCCAGGCTGATCTCGCCCTGCACCAGATCCATGGCCCCACCCAGCGCCTGCAGCAAGGTTGATTTGCCGGCGCCGTTGCGGCCCAGCACGGCAATGCGCTCGCCCGGCTGGATATc < 1:31494/136‑1 (MQ=255) gTGGGCCATGGCGATGCCGTCCAGGCTGATCTCGCCCTGCACCAGATCCATGGCCCCACCCAGCGCCTGCAGCAAGGTTGATTTGCCGGCGCCGTTGCGGCCCAGCACGGCAATGCGCTCGCGCGGCggggtgggc > 1:121661/1‑130 (MQ=255) tGGGCCATGGCGATGCCGTCCAGGCTGATCTCGCCCTGCACCAGATCCATGGCCCCACCCAGCGCCTGCAGCAAGGTTGATTTGCCGGCGCCGTTGCGGCCCAGCACGGCAATGCGCTCGCCCGGCTGGATATCCa < 1:217462/136‑1 (MQ=255) cATGGCGATGCCGTCCAGGCTGATCTCGCCCTGCACCAGATCCATGGCCCCACCCAGCGCCTGCAGCAAGGTTGATTTGCCGGCGCCGTTGCGGCCCAGCACGGCAATGCGCTCGCCCGGCTGGATATCCA‑GCCgg < 1:284272/136‑1 (MQ=255) aTGGCGATGCCGTCCAGGCTGATCTCGCCCTGCACCAGATCCATGGCCCCACCCAGCGCCTGCAGCAAGGTTGATTTGCCGGCGCCGTTGCGGCCCAGCACGGCAATGCGCTCGCCCGGCGGGTTATACA‑GCCCGc > 1:568555/1‑136 (MQ=255) tGGCGATGCCGTCCAGGCTGATCTCGCCCTGCACCAGATCCATGGCCCCACCCAGCGCCTGCAGCAAGGTTGATTTGCCGGCGCCGTTGCGGCCCAGCACGGCAATGCGCTCGCCCCGCGGGATATACA‑GCCCGcc > 2:831862/1‑136 (MQ=255) cGATGCCGTCCAGGCTGATCTCGCCCTGCACCAGATCCATGGCCCCACCCAGCGCCTGCAGCAAGGTTGATTTGCCGGCGCCGTTGCGGCCCAGCACGGCAATGCGCTCGCCCGGCTGGATATCCA‑GCCGGCCAAt < 2:222205/136‑1 (MQ=255) cGATGCCGTCCAGGCTGATCTCGCCCTGCACCAGATCCATGGCCCCACCCAGCGCCTGCAGCAAGGTTGATTTGCCGGCGCCGTTGCGGCCCAGCACGGCAATGCGCTCGCCCGGCTGGATATCCA‑GCCGGCCAAt < 2:1020727/136‑1 (MQ=255) gATGCCGTCCAGGCTGATCTCGCCCTGCACCAGATCCATGGCCCCACCCAGCGCCTGCAGCAAGGTTGATTTGCCGGCGCCGTTGCGGCCCAGCACGGCAATGCGCTCGCGCGGGGGGGGATACA‑GCCGGccccgg > 1:1003393/1‑132 (MQ=255) gATGCCGTCCAGGCTGATCTCGCCCTGCACCAGATCCATGGCCCCACCCAGCGCCTGCAGCAAGGTTGATTTGCCGGCGCCGTTGCGGCCCAGCACGGCAATGCGCTCGCCCGGCGGGGGATACA‑CCCGGCCaagg > 1:1062768/1‑134 (MQ=255) ccGTCCAGGCTGATCTCGCCCTGCACCAGATCCATGGCCCCACCCAGCGCCTGCAGCAAGGTTGATTTGCCGGCGCCGTTGCGGCCCAGCACGGCAATGCGCTCGCCCGGCTGGGTATACA‑GCCGGCCCAAGTTCa > 1:1069551/1‑136 (MQ=255) ccGTCCAGGCTGATCTCGCCCTGCACCAGATCCATGGCCCCACCCAGCGCCTGCAGCAAGGTTGATTTGCCGGCGCCGTTGCGGCCCAGCACGGCAATGCGCTCGCCCGGCTGGATATCCA‑GCCGGCCAATGTTCa < 2:822821/136‑1 (MQ=255) ccGTCCAGGCTGATCTCGCCCTGCACCAGATCCATGGCCCCACCCAGCGCCTGCAGCAAGGTTGATTTGCCGGCGCCGTTGCGGCCCAGCACGGCAATGCGCTCGCCCGGCTGGATATCCA‑GCCGGCCAATGTTCa < 2:870224/136‑1 (MQ=255) gTCCAGGCTGATCTCGCCCTGCACCAGATCCATGGCCCCACCCAGCGCCTGCAGCAAGGTTGATTTGCCGGCGCCGTTGCGGCCCAGCACGGCAATGCGCTCGCCCGGCTGGCTATCCA‑GCCCGCCCATGTTCAGc > 2:1262299/1‑136 (MQ=255) tCCAGGCTGATCTCGCCCTGCACCAGATCCATGGCCCCACCCAGCGCCTGCAGCAAGGTTGATTTGCCGGCGCCGTTGCGGCCCAGCACGGCAATGCGCTCGCCCGGCTGGATATCCA‑GCCGGCCAATGTTCAGCa < 1:523187/136‑1 (MQ=255) aGGCTGATCTCGCCCTGCACCAGATCCATGGCCCCACCCAGCGCCTGCAGCAAGGTTGATTTGCCGGCGCCGTTGCGGCCCAGCACGGCAATGCGCTCGCCCGGCGGGATTTCCA‑CCCGGCCCATGTTGAGCAcgg > 2:37050/1‑134 (MQ=255) gATCTCGCCCTGCACCAGATCCATGGCCCCACCCAGCGCCTGCAGCAAGGTTGATTTGCCGGCGCCGTTGCGGCCCAGCACGGCAATGCGCTCGCCCGGCGGGATATCAC‑GCCGGCCAATGTTGAGCACTGGCggg > 2:1026510/1‑136 (MQ=255) gATCTCGCCCTGCACCAGATCCATGGCCCCACCCAGCGCCTGCAGCAAGGTTGATTTGCCGGCGCCGTTGCGGCCCAGCACGGCAATGCGCTCGCCCCGCGGGGTATACA‑CCCCGCCCAAGTTAAGCACAGGCggg > 2:1341276/1‑136 (MQ=255) aTCTCGCCCTGCACCAGATCCATGGCCCCACCCAGCGCCTGCAGCAAGGTTGATTTGCCGGCGCCGTTGCGGCCCAGCACGGCAATGTGCTCGCCCGGCTGGATATCCA‑GCCGGCCAATGTTCAGCACTGGCGGGt < 2:1054921/136‑1 (MQ=255) aTCTCGCCCTGCACCAGATCCATGGCCCCACCCAGCGCCTGCAGCAAGGTTGATTTGCCGGCGCCGTTGCGGCCCAGCACGGCAATGCGCTCGCCCGGCTGGATATCCA‑GCCGGCCAATGTTCAGCACTGGCGGGt < 2:69509/136‑1 (MQ=255) tcGCCCTGCACCAGATCCATGGCCCCACCCAGCGCCTGCAGCAAGGTTGATTTGCCGGCGCCGTTGCGGCCCAGCACGGCAATGCGCTCGCCCGGGGGGGTTTACA‑GCCGGCCCAAGTTGAGCACTGGGGGGGAGt > 1:462334/1‑136 (MQ=255) cGCCCTGCACCAGATCCATGGCCCCACCCAGCGCCTGCAGCAAGGTTGATTTGCCGGCGCCGTTGCGGCCCAGCACGGCAATGCGCTCGCCCGGGGGGGTATACA‑CCCGGCCAATGTTGAGGCCAGGCggggtgtg > 2:1125836/1‑131 (MQ=255) cGCCCTGCACCAGATCCATGGCCCCACCCAGCGCCTGCAGCAAGGTTGATTTGCCGGCGCCGTTGCGGCCCAGCACGGCAATGCGCTCGCCCGGCGGGGTATACA‑GCCCGCCCATGTTCAGCACTGGCGGGTAGtg > 2:241874/1‑135 (MQ=255) cGCCCTGCACCAGATCCATGGCCCCACCCAGCGCCTGCAGCAAGGTTGATTTGCCGGCGCCGTTGCGGCCCAGCACGGCAATGCGCTCGCCCGGCGGGGGATCCC‑GCCCGCCAATGTCCAGCGCAgggggggtggg > 1:1353927/1‑131 (MQ=255) cGCCCTGCACCAGATCCATGGCCCCACCCAGCGCCTGCAGCAAGGTTGATTTGCCGGCGCCGTTGCGGCCCAGCACGGCAATGCGCTCGCCCCGCGGGATATACA‑GCCGGCCAAAGTTAAGCCCAGGCGGGGAGta > 2:1236242/1‑135 (MQ=255) ccAGATCCATGGCCCCACCCAGCGCCTGCAGCAAGGTTGATTTGCCGGCGCCGTTGCGGCCCAGCACGGCAATGCGCTCGCCCGGCGGGGTATACA‑CCCGGCCCAAGTTCAGGACGGGCGGGGAATCTTCgggggt > 2:1077573/1‑131 (MQ=255) tGGCCCCACCCAGCGCCTGCAGCAAGGTTGATTTGCCGGCGCCGTTGCGGCCCAGCACGGCAATGCGCTCGCCCGGCTGGATATCCA‑GCCGGCCAATGTTCAGCACTGGCGGGTAGTCTTCGCTGTAGCGGAAGtt < 2:784552/136‑1 (MQ=255) tGGCCCCACCCAGCGCCTGCAGCAAGGTTGATTTGCCGGCGCCGTTGCGGCCCAGCACGGCAATGCGCGCGCCCGGCTGGATATCCA‑GCCGGCCAATGTTCAGCACTGGCGGGTAGTCTTCGCTGTAGCGGAAGtt < 1:720118/136‑1 (MQ=255) ggCCCCACCCAGCGCCTGCAGCAAGGTTGATTTGCCGGCGCCGTTGCGGCCCAGCACGGCAATGCGCTCGCCCGGCTGGATATCCA‑GCCGGCCAATGTTCAGCACTGGCGGGTAGTCTTCGCTGTAGCGGAAGTTg < 2:744514/136‑1 (MQ=255) gCCCCACCCAGCGCCTGCAGCAAGGTTGATTTGCCGGCGCCGTTGCGGCCCAGCACGGCAATGCGCTCGCGCGGGGGGATATACA‑GCCGGCCAAAGTTAAGCCCGGGCGGGTTTTCTTCGCGGTAGGGGGAGTTgg > 2:71918/1‑136 (MQ=255) gCCCCACCCAGCGCCTGCAGCAAGGTTGATTTGCCGGCGCCGTTGCGGCCCAGCACGGCAATGCGCTCGCGCCGCGGGATTTCAC‑GCCGGGCAATGTTGAGCACAGGGGGGTAGTCTTAGCGGTAGGGGGAggttg > 2:643984/1‑132 (MQ=255) gCCCCACCCAGCGCCTGCAGCAAGGTTGATTTGCCGGCGCCGTTGCGGCCCAGCACGGCAATGCGCTCGCCCGGCTGGATATCCA‑GCCGGCCAATGTTCAGCACTGGCGGGTAGTCTTCGCTGTAGCGGAAGTTgg < 1:1234965/136‑1 (MQ=255) caGCGCCTGCAGCAAGGTTGATTTGCCGGCGCCGTTGCGGCCCAGCACGGCAATGCGCTCGCCCGGGGGGGTATACA‑GCCCGGCAATGTTCAGCACAGGCGGGGAGTGTTGGCTGGTGGGGAAGTTGGCCTGgcgc > 2:1055695/1‑136 (MQ=255) caGCGCCTGCAGCAAGGTTGATTTGCCGGCGCCGTTGCGGCCCAGCACGGCAATGCGCTCGCCCCGCGGGATATACA‑GCCCGCCAAAGTTCAGCCCGGGCGGGGAGTCTTCGCAGTTGCGGAAGTTTGCCGGgcgc > 2:742979/1‑136 (MQ=255) cTGCAGCAAGGTTGATTTGCCGGCGCCGTTGCGGCCCAGCACGGCAATGCGCTCGCCCGGCGGGGTATGCA‑GCCCGCCCAAGTTCAGCACAGGCGGGGGGTCGTCGCTGTAGCGGGAGTTGGCCGGGCGCCGgggg > 1:663072/1‑136 (MQ=255) cTGCAGCAAGGTTGATTTGCCGGCGCCGTTGCGGCCCAGCACGGCAATGCGCTCGCCCGGCGGGCTATCCACCCCGCCCAATG‑TGAGCACAGGGGGGGTGTGTTGGCTGTAGGGGAAGGTGGGCTGGCGGAGGCgg > 2:1159901/1‑136 (MQ=255) gTTGATTTGCCGGCGCCGTTGCGGCCCAGCACGGCAATGCGCTCGCCCGGCTGGATATCCA‑GCCGGCCAATGTTCAGCACTGGCGGGTAGTCTTCGCTGTAGCGGAAGTTGGCCTGGCGCAGGCGGTACTCGCCGt < 1:1341276/136‑1 (MQ=255) gTTGATTTGCCGGCGCCGTTGCGGCCCAGCACGGCAATGCGCTCGCCCGGCTGGATATCCA‑GCCGGCCAATGTTCAGCACTGGCGGGTAGTCTTCGCTGTAGCGGAAGTTGGCCTGGCGCAGGCGGTACTCGCCGt < 1:1026510/136‑1 (MQ=255) tGCCGGCGCCGTTGCGGCCCAGCACGGCAATGCGCTCGCCCGGCGGGGTTTACC‑CCCCGCCCAAGTTGAGCACGGGGCGGGAGTCTTGGCTGTTGGCGGTCTTGGGCTGGCGCGGGCGGGACACGCCGAGAATGGc > 2:501740/1‑136 (MQ=255) tGCCGGCGCCGTTGCGGCCCAGCACGGCAATGCGCTCGCCCGGCGGGGTATACA‑GCCCGCCAATGTTGAGCACTGGCGGGTTGTCTTGGCTGTAGCGGAAGTTGGGCTGGCGCAGGCGGTTCTCGCCGTGAATggg > 2:753274/1‑135 (MQ=255) gCCGGCGCCGTTGCGGCCCAGCACGGCAATGCGCTCGCCCGGCTGGATATCCA‑GCCGGCCAATGTTCAGCACTGGCGGGTAGTCTTCGCTGTAGCGGAAGTTGGCCTGGCGCAGGCGGTACTCGCCGTGAATGGcc < 1:37050/136‑1 (MQ=255) gTTGCGGCCCAGCACGGCAATGCGCTCGCCCGGCTGGATATCCA‑GCCGGCCAATGTTCAGCACTGGCGGGTAGTCTTCGCTGTAGCGGAAGTTGGCCTGGCGCAGGCGGTACTCGCCGTGAATGGCCGGCAGGTGc < 2:281497/136‑1 (MQ=255) gTTGCGGCCCAGCACGGCAATGCGCTCGCCCGGCTGGATATCCA‑GCCGGCCAATGTTCAGCACTGGCGGGTAGTCTTCGCTGTAGCGGAAGTTGGCCTGGCGCAGGCGGTACTCGCCGTGAATGGCCGGCAGGTGc < 1:13784/136‑1 (MQ=255) gTTGCGGCCCAGCACGGCAATGCGCGCGCCCGGCTGGATATCCA‑GCCGGCCAATGTTCAGCACTGGCGGGTAGTCTTCGCTGTAGCGGAAGTTGGCCTGGCGCAGGCGGTACTCGCCGTGAATGGCCGGCAGGTGc < 1:955355/136‑1 (MQ=255) cccAGCACGGCAATGCGGTCGCCCGGCTGTATATCCA‑GCCGGCCAATGTTCAGCACTGGCGGGTAGTCTTCGCTGTAGCGGAAGTTGGCCTGGCGCAGGCGGTACTCGCCGTGAATGGCCGGCAGGTGCACGCggg < 2:1163957/136‑1 (MQ=255) cccAGCACGGCAATGCGCTCGCCCGGCTGGATATCCA‑GCCGGCCAATGTTCAGCACTGGCGGGTAGTCTTCGCTGTAGCGGAAGTTGGCCTGGCGCAGGCGGTACTCGCCGTGAATGGCCGGCAGGTGCACGCggg < 1:1030712/136‑1 (MQ=255) cccAGCACGGCAATGCGCTCGCCCGGCTGGATATCCA‑GCCGGCCAATGTTCAGCACTGGCGGGTAGTCTTCGCTGTAGCGGAAGTTGGCCTGGCGCAGGCGGTACTCGCCGTGAATGGCCGGCAGGTGCACGCggg < 1:831862/136‑1 (MQ=255) cccAGCACGGCAATGCGCTCGCCCGGCTGGATATCCA‑GCCGGCCAATGTTCAGCACTGGCGGGTAGTCTTCGCTGTAGCGGAAGTTGGCCTGGCGCAGGCGGTACTCGCCGTGAATGGCCGGCAGGTGCACGCggg < 2:662565/136‑1 (MQ=255) ggCAATGCGCTCGCCCGGCTGTATATCCA‑GCCGGCCAATGTTCAGCACTGGCGGGTAGTCTTCGCTGTAGCGGAAGTTGGCCTGGCGCAGGCGGTACTCGCCGTGAATGGCCGGCAGGTGCACGCGGGCTTCGCCt < 1:1081772/136‑1 (MQ=255) gcTCGCCCGGGGGGATATACA‑GCCGGCCAATGTTGAGCACTGGGGGGGTGTGTTAGGGGTTGCGGAAGTTGGGCGGGCGCAGGCGGTACTCGCCGGGAATGGCCGGCAGGTGCACGCGGGCGTCGGCGTGGGGGTg > 2:449156/1‑136 (MQ=255) gcGCCCGGCTGGATATCCA‑GCCGGCCAATGTTCAGCACTGGCGGGTAGTCTTCGCTGTAGCGGAAGTTGGCCTGGCGCAGGCGGTACTCGCCGTGAATGGCCGGCAGGTGCACGCGGGCTTCGCCTTCGGGGTGAt < 2:212320/135‑1 (MQ=255) gCCCGGCTGGATATCCA‑GCCGGCCAATGTTCAGCACTGGCGGGTAGTCTTCGCTGTAGCGGAAGTTGGCCTGGCGCAGGCGGTACTCGCCGTGAATGGCCGGCAGGTGCACGCGGGCTTCGCCTTCGGGGTGATCg < 1:970586/136‑1 (MQ=255) gCTGGATATCCA‑GCCGGCCAATGTTCAGCACTGGCGGGTAGTCTTCGCTGTAGCGGAAGTTGGCCTGGCGCAGGCGGTACTCGCCGTGAATGGCCGGCAGGTGCACGCGGGCTTCGCCTTCGGGGTGATCGACCgg < 1:1236242/136‑1 (MQ=255) gCTGGATATCCA‑GCCGGCCAATGTTCAGCACTGGCGGGTAGTCTTCGCTGTAGCGGAAGTTGGCCTGGCGCAGGCGGTACTCGCCGTGAATGGCCGGCAGGTGCACGCGGGCTTCGCCTTCGGGGTGATCGACCgg < 2:1308357/136‑1 (MQ=255) gCTGGATATCCA‑GCCGGCCAATGTTCAGCACTGGCGGGTAGTCTTCGCTGTAGCGGAAGTTGGCCTGGCGCAGGCGGTACTCGCCGTGAATGGCCGGCAGGTGCACGCGGGCTTCGCCTTCGGGGTGATCGACCgg < 1:93693/136‑1 (MQ=255) | AGGTGGGCCATGGCGATGCCGTCCAGGCTGATCTCGCCCTGCACCAGATCCATGGCCCCACCCAGCGCCTGCAGCAAGGTTGATTTGCCGGCGCCGTTGCGGCCCAGCACGGCAATGCGCTCGCCCGGCTGGATATCCA‑GCCGGCCAATGTTCAGCACTGGCGGGTAGTCTTCGCTGTAGCGGAAGTTGGCCTGGCGCAGGCGGTACTCGCCGTGAATGGCCGGCAGGTGCACGCGGGCTTCGCCTTCGGGGTGATCGACCGG > NC_002947/923520‑923782 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |