Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_002947	2,533,751	C→T	S14L (TCG→TTG)	PP_2222 →	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	2,533,751	0	C	T	100.0%	89.5 / NA	26	S14L (TCG→TTG)	PP_2222	hypothetical protein
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (15/11);  total (15/11)

GAAACACTGCTGTACCGCAATGCGCTGTTTCCGCACCACTGTCAGGCGTATCATGGCTTTATCGTTAGGGTGCTAATTAAATTCCGGAGGGGATGATGAATAGCCGAGTCGATGTGGCTGTGATGATAGGGTCGGGTGTGCCGGCCACCTTGCAGGCGCTGGGCAAGCGTATTTGCTGGGTAGTGCTGGTCAATGGCGAGCGTCGCGGCACCGCCTTCGCCACGCGCGAAGAAGCGCTTGAGTGCCAGGCTGCCTGGCAGCAGC  >  NC_002947/2533619‑2533882                                                                                                                                     |                                                                                                                                    gAAACACTGCTGTACCGCAATGCGCTGTTTCCGCACCACTGTCAGGCGTATCATGGCTTTATCGTTAGGGTGCTAATTAAATTCCGGAGGGGATGATGAATAGCCGAGTCGATGTGGCTGTGATGATAGGGTTggg                                                                                                                                  >  2:1260329/1‑136 (MQ=255)                     tGCGCTGTTTCCGCACCACTGTCAGGCGTATCATGGCTTTATCGTTAGGGTGCTAATTAAATTCCGGAGGGGATGATGAATAGCCGAGTCGATGTGGCTGTGATGATAGGGTTGGGTGTGCCGGCCACCTTGCAgg                                                                                                              >  2:148193/1‑136 (MQ=255)                                  caccacTGTCAGGCGTATCATGGCTTTATCGTTAGGGTGCTAATTAAATTCCGGAGGGGATGATGAATAGCCGAGTCGATGTGGCTGTGATGATAGGGTTGGGTGTGCCGGCCACCTTGCAGGCGCTGGGCAAGCg                                                                                                 <  1:1164304/136‑1 (MQ=255)                                     cacTGTCAGGCGTATCATGGCTTTATCGTTAGGGTGCTAATTAAATTCCGGAGGGGATGATGAATAGCCGAGTCGATGTGGCTGTGATGATAGGGTTGGGTGTGCCGGCCACCTTGCAGGCGCTGGGCAAGCGTAt                                                                                              <  2:44884/136‑1 (MQ=255)                                         gTCAGGCGTATCATGGCTTTATCGTTAGGGTGCTAATTAAATTCCGGAGGGGATGATGAATAGCCGAGTCGATGTGGCTGTGATGATAGGGTTGGGTGTGCCGGCCACCTTGCAGGCGCTGGGCAAGCGTATTTgg                                                                                          >  1:1152939/1‑135 (MQ=255)                                         gTCAGGCGTATCATGGCTTTATCGTTAGGGTGCTAATTAAATTCCGGAGGGGATGATGAATAGCCGAGTCGATGTGGCTGTGATGATAGGGTTGGGTGTGCCGGCCACCTTGCAGGCGCTGGGCAAGCGTATTTGc                                                                                          >  1:883438/1‑136 (MQ=255)                                          tCAGGCGTATCATGGCTTTATCGTTAGGGTGCTAATTAAATTCCGGAGGGGATGATGAATAGCCGAGTCGATGTGGCTGTGATGATAGGGTTGGGTGTGCCGGCCACCTTGCAGGCGCTGGGCAAGCGTATTTGCt                                                                                         <  2:3790/136‑1 (MQ=255)                                          tCAGGCGTATCATGGCTTTATCGTTAGGGTGCTAATTAAATTCCGGAGGGGATGATGAATAGCCGAGTCGATGTGGCTGTGATGATAGGGTTGGGTGTGCCGGCCACCTTGCAGGCGCTGGGCAAGCGTATTTGCt                                                                                         <  2:237556/136‑1 (MQ=255)                                          tCAGGCGTATCATGGCTTTATCGTTAGGGTGCTAATTAAATTCCGGAGGGGATGATGAATAGCCGAGTCGATGTGGCTGTGATGATAGGGTTGGGTGTGCCGGCCACCTTGCAGGCGCTGGGCAAGCGTATTTGCt                                                                                         <  2:1118167/136‑1 (MQ=255)                                               cGTATCATGGCTTTATCGTTAGGGTGCTAATTAAATTCCGGAGGGGATGATGAATAGCCGAGTCGATGTGGCTGTGATGATAGGGTTGGGTGTGCCGGCCACCTTGCAGGCGCTGGGCAAGCGTATTTGCTGGGTa                                                                                    <  1:1173261/136‑1 (MQ=255)                                                    cATGGCTTTATCGTTAGGGTGCTAATTAAATTCCGGAGGGGATGATGAATAGCCGAGTCGATGTGGCTGTGATGATAGGGTTGGGTGTGCCGGCCACCTTGCAGGCGCTGGGCAAGCGTATTTGCTGGGTAGTGCt                                                                               >  1:936625/1‑136 (MQ=255)                                                    cATGGCTTTATCGTTAGGGTGCTAATTAAATTCCGGAGGGGATGATGAATAGCCGAGTCGATGTGGCTGTGATGAAAGGGTTGGGTGTGCCGGCCACCTTGCAGGCGCTGGGCAAGCGTATTTGCTGGGTAGTGCt                                                                               >  2:1128140/1‑136 (MQ=255)                                                              tCGTTAGGGTGCTAATTAAATTCCGGAGGGGATGATGAATAGCCGAGTCGATGTGGCTGTGATGATAGGGTTGGGTGTGCCGGCCACCTTGCAGGCGCTGGGCAAGCGTATTTGCTGGGTAGTGCTGGTCAATGGc                                                                     <  1:1153114/136‑1 (MQ=255)                                                                      gTGCTAATTAAATTCCGGAGGGGATGATGAATAGCCGAGTCGATGTGGCTGTGATGATAGGGTTGGGTGTGCCGGCCACCTTGCAGGCGCTGGGCAAGCGTATTTGCTGGGTAGTGCTGGTCAATGGCGAGCGTcg                                                             >  2:864904/1‑136 (MQ=255)                                                                         cTAATTAAATTCCGGAGGGGATGATGAATAGCCGAGTCGATGTGGCTGTGATGATAGGGTTGGGTGTGCCGGCCACCTTGCAGGCGCTGGGCAAGCGTATTTGCTGGGTAGTGCTGGTCAATGGCGAGCGTCGCgg                                                          <  1:148193/136‑1 (MQ=255)                                                                           aaTTAAATTCCGGAGGGGATGATGAATAGCCGAGTCGATGTGGCTGTGATGATAGGGTTGGGTGTGCCGGCCACCTTGCAGGCGCTGGGCAAGCGTATTTGCTGGGTAGTGCTGGTCAATGGCGAGCGTCGCGGCa                                                        >  1:1077946/1‑136 (MQ=255)                                                                            aTTAAAATCCGGAGGCGATGATCAATAGCCGAGTCCATGTGGCTGTGATGATAGGGTTGGGTGTGCCGGCCACCTAGCACGCGCAGCCCAAGCCTATTTCCAGGGTAGAGCTGGTCAATGGCGAGCCTCGCGGCAc                                                       >  1:841114/1‑136 (MQ=255)                                                                                   tCCGGAGGGGATGATGAATAGCCGAGTCGATGTGGCTGTGATGATAGGGTTGGGTGTGCCGGCCACCTTGCAGGCGCTGGGCAAGCGTATTTGCTGGGTAGTGCTGGTCAATGGCGAGCGTCGCGGCACCGCCTTc                                                >  2:1070625/1‑136 (MQ=255)                                                                                               gatgaATAGCCGAGTCGATGTGGCTGTGATGATAGGGTTGGGTGTGCCGGCCACCTTGCAGGCGCTGGGCAAGCGTATTTGCTGGGTAGTGCTGGTCAATGGCGAGCGTCGCGGCACCGCCTTCGCCACGCGCgaa                                    >  1:787835/1‑136 (MQ=255)                                                                                                       gCCGAGTCGATGTGGCTGTGATGATAGGGTTGGGTGTGCCGGCCACCTTGCAGGCGCTGGGCAAGCGTATTTGCTGGGTAGTGCTGGTCAATGGCGAGCGTCGCGGCACCGCCTTCGCCACGCGCGAAGAAGCGCt                            >  1:1089436/1‑136 (MQ=255)                                                                                                              cGATGTTGCTGTGATGATAGGGTTGGGTGTGCCGGCCACCTTGCAGGCGCTGGGCAAGCGTATTTGCTGGGTAGTGCTGGTCAATGGCGAGCGTCGCGGCACCGCCTTCGCCACGCGCGAAGAAGCGCTTGAGTGc                     >  1:1185821/1‑136 (MQ=255)                                                                                                                aTGTGGCTGTGATGATAGGGTTGGGTGTGCCGGCCACCTTGCAGGCGCTGGGCAAGCGTATTTGCTGGGTAGTGCTGGTCAATGGCGAGCGTCGCGGCACCGCCTTCGCCACGCGCGAAGAAGCGCTTGAGTGccc                   >  1:468509/1‑135 (MQ=255)                                                                                                                            tgatAGGGTTGGGTGTGCCGGCCACCTTGCAGGCGCTGGGCAAGCGTATTTGCTGGGTAGTGCTGGTCAATGGCGAGCGTCGCGGCACCGCCTTCGCCACGCGCGAAGAAGCGCTTGAGTGCCAGGCTGCCTGgca       >  2:716605/1‑136 (MQ=255)                                                                                                                             gatAGGGTTGGGTGTGCCGGCCACCTTGCAGGCGCTGGGCAAGCGTATTTGCTGGGTAGTGCTGGTCAATGGCGAGCGTCGCGGCACCGCCTTCGCCACGCGCGAAGAAGCGCTTGAGTGCCAGGCTGCCTGgcag      <  1:199169/136‑1 (MQ=255)                                                                                                                                aGGGTTGGGTGTGCCGGCCACCTTGCAGGCGCTGGGCAAGCGTATTTGCTGGGTAGTGCTGGTCAATGGCGAGCGTCGCGGCACCGCCTTCGCCACGCGCGAAGAAGCGCTTGAGTGCCAGGCTGCCTGgcagcag   <  2:1077946/136‑1 (MQ=255)                                                                                                                                 gggTTGGGTGTGCCGGCCACCTTGCAGGCGCTGGGCAAGCGTATTTGCTGGGTAGTGCTGGTCAATGGCGAGCGTCGCGGCACCGCCTTCGCGACGCGCGAAGAAGCGCTTGAGTGCCAGGCTGCCTGgcagcagc  <  1:1128140/136‑1 (MQ=255)                                                                                                                                     |                                                                                                                                    GAAACACTGCTGTACCGCAATGCGCTGTTTCCGCACCACTGTCAGGCGTATCATGGCTTTATCGTTAGGGTGCTAATTAAATTCCGGAGGGGATGATGAATAGCCGAGTCGATGTGGCTGTGATGATAGGGTCGGGTGTGCCGGCCACCTTGCAGGCGCTGGGCAAGCGTATTTGCTGGGTAGTGCTGGTCAATGGCGAGCGTCGCGGCACCGCCTTCGCCACGCGCGAAGAAGCGCTTGAGTGCCAGGCTGCCTGGCAGCAGC  >  NC_002947/2533619‑2533882

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑