Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_002947	3,827,925	G→A	S29F (TCC→TTC)	ptxS ←	2‑ketogluconate utilization repressor

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	3,827,925	0	G	A	100.0%	164.5 / NA	46	S29F (TCC→TTC)	ptxS	2‑ketogluconate utilization repressor
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (17/29);  total (17/29)

GATCAGTCGCGTCTGGCCGCGCTTTAGGCCGCGAGCCATCTGGTTGGGGCGATAGCCGAGGCGTTCGATGACCTCTTCCAGCCGCTTGGCCGTGGCTTCGGCCAGCAATTGGCGGTCGCCACCGATGTAACGGGAGACGGTGGCTTTGGAAACGCCAGCAACACGCGCCACTTCACTGATGGTGACCCGTTCGCGGGTATGGGCAGGCGCGTCGGTCATGGGTTTTCCTTATGATTGTTTTAATAGTGATGAAACCGGTTTCA  >  NC_002947/3827792‑3828054                                                                                                                                      |                                                                                                                                  gATCAGTCGCGTCTGGCCGCGCTTTAGGCCGCGAGCCATCTGGTTGGGGCGATAGCCGAGGCGTTCGATGACCTCTTCCAGCCGCTTGGCCGTGGCTTCGGCCAGCAATTGGCGGTCGCCACCGATGTAACGGaag                                                                                                                                 >  1:672926/1‑136 (MQ=255)   tCAGTCGCGTCTGGCCGCGCTTTAGGCCGCGAGCCATCTGGTTGGGGCGATAGCCGAGGCGTTCGATGACCTCTTCCAGCCGCTTGGCCGTGGCTTCGGCCAGCAATTGGCGGTCGCCACCGATGTAACGGAAGAc                                                                                                                               <  2:293126/136‑1 (MQ=255)     aGTCGCGTCTGGCCGCGCTTTAGGCCGCGAGCCATCTGGTTGGGGCGCTAGCCGAGGCGTTCGATGCCCTCTTCCAGCCGCTTGGCCGTGGCTTCGGCCAGCAATTGGCGGTCGCCACCGATGTAACGGAAGACgg                                                                                                                             >  2:468839/1‑136 (MQ=255)        cgcgTCTGGCCGCGCTTTAGGCCGCGAGCCATCTGGTTGGGGCGATAGCCGAGGCGTTCGATGACCTCTTCCAGCCGCTTGGCCGTGGCTTCGGCCAGCAATTGGCGGTCGCCACCGATGTAACGGAAGACGGTgg                                                                                                                          >  2:915176/1‑136 (MQ=255)            tCTGGCCGCGCTTTAGGCCGCGAGCCATCTGGTTGGGGCGATAGCCGAGGCGTTCGATGACCTCTTCCAGCCGCTTGGCCGTGGCTTCGGCCAGCAATTGGCGGTCGCCACCGATGTAACGGAAGACGGTGGCttt                                                                                                                      <  1:265599/136‑1 (MQ=255)            gcTGGCCGCGCTTTAGGCCGCGAGCCATCTGGTTGGGGCGATAGCCGAGGCGTTCGATGACCTCTTCCAGCCGCTTGGCCGTGGCTTCGGCCAGCAATTGGCGGTCGCCACCGATGTAACGGAAGACGGTGGCttt                                                                                                                      <  1:256050/135‑1 (MQ=255)                    cgcTTTAGGCCGCGAGCCATCTGGTTGGGGCGATAGCCGAGGCGTTCGATGACCTCTTCCAGCCGCTTGGCCGTGGCTTCGGCCAGCAATTGGCGGTCGCCACCGATGTAACGGAAGACGGTGGCTTTGGAAACGc                                                                                                              <  1:1064876/136‑1 (MQ=255)                      cTTTAGGCCGCGAGCCATCTGGTTGGGGCGATAGCCGAGGCGTTCGATGACCTCTTCCAGCCGCTTGGCCGTGGCTTCGGCCAGCAATTGGCGGTCGCCACCGATGTAACGGAAGACGGTGGCTTTGGAAACGCCa                                                                                                            <  2:1004491/136‑1 (MQ=255)                         tAGGCCGCGAGCCATCTGGTTGGGGCGATAGCCGAGGCGTTCGATGACCTCTTCCAGCCGCTTGGCCGTGGCTTCGGCCAGCAATTGGCGGTCGCCACCGATGTAACGGAAGACGGTGGCTTTGGAAACGCCAGCa                                                                                                         <  2:3655/136‑1 (MQ=255)                              cgcgAGCCATCTGGTTGGGGCGATAGCCGAGGCGTTCGATGACCTCTTCCAGCCGCTTGGCCGTGGCTTCGGCCAGCAATTGGCGGTCGCCACCGATGTAACGGAAGACGGTGGCTTTGGAAACGCCAGCAACAcg                                                                                                    <  2:885150/136‑1 (MQ=255)                                cgAGCCATCTGGTTGGGGCGATAGCCGAGGCGTTCGATGACCTCTTCCAGCCGCTTGGCCGTGGCTTCGGCCAGCAATTGGCGGTCGCCACCGATGTAACGGAAGACGGTGGCTTTGGAAACGCCAGCAACAcgcg                                                                                                  <  1:193743/136‑1 (MQ=255)                                 gAGCCATCTGGTTGGGGCGATAGCCGAGGCGTTCGATGACCTCTTCCAGCCGCTTGGCCGTGGCTTCGGCCAGCAATTGGCGGTCGCCACCGATGTAACGGAAGACGGTGGCTTTGGAAACGCCAGCAACACgcgc                                                                                                 <  1:451335/136‑1 (MQ=255)                                  aGCCATCTGGTTGGGGCGATAGCCGAGGCGTTCGATGACCTCTTCCAGCCGCTTGGCCGTGGCTTCGGCCAGCAATTGGCGGTCGCCACCGATGTAACGGAAGACGGTGGCTTTGGAAACGCCAGCAACACGCGcc                                                                                                >  1:32134/1‑136 (MQ=255)                                   gCCATCTGGTTGGGGCGATAGCCGAGGCGTTCGATGACCTCTTCCAGCCGCTTGGCCGTGGCTTCGGCCAGCAATTGGCGGTCGCCACCGATGTAACGGAAGACGGTGGCTTTGGAAACGCCAGCAACACGCGCCa                                                                                               >  2:680488/1‑136 (MQ=255)                                   gCCATCTGGTTGGGGCGATAGCCGAGGCGTTCGATGACCTCTTCCAGCCGCTTGGCCGTGGCTTCGGCCAGCAATTGGCGGTCGCCACCGATGTAACGGAAGACGGTGGCTTTGGAAACGCCAGCAACACGCGCCa                                                                                               <  1:56168/136‑1 (MQ=255)                                   gCCATCTGGTTGGGGCGATAGCCGAGGCGTTCGATGACCTCTTCCAGCCGCTTGGCCGTGGCTTCGGCCAGCAATTGGCGGTCGCCACCGATGTAACGGAAGACGGTGGCTTTGGAAACGCCAGCAACACGCGCCa                                                                                               >  2:506775/1‑136 (MQ=255)                                   gCCATCTGGTTGGGGCGATAGCCGAGGCGTTCGATGACCTCTTCCAGCCGCTTGGCCGTGGCTTCGGCCAGCAATTGGCGGTCGCCACCGATGTAACGGAAGACGGTGGCTTTGGAAACGCCAGCAACACGCGCCa                                                                                               >  2:335744/1‑136 (MQ=255)                                   gCCATCTGGTTGGGGCGATAGCCGAGGCGTTCGATGACCTCTTCCAGCCGCTTGGCCGTGGCTTCGGCCAGCAATTGGCGGTCGCCACCGATGTAACGGAAGACGGTGGCTTTGGAAACGCCAGCAACACGCGCCa                                                                                               >  1:1171750/1‑136 (MQ=255)                                    ccATCTGGTTGGGGCGATAGCCGAGGCGTTCGATGACCTCTTCCAGCCGCTTGGCCGTGGCTTCGGCCAGCAATTGGCGGTCGCCACCGATGTAACGGAAGACGGTGGCTTTGGAAACGCCAGCAACACGCGCCAc                                                                                              >  2:270775/1‑136 (MQ=255)                                      aTCTGGTTGGGGCGATAGCCGAGGCGTTCGATGACCTCTTCCAGCCGCTTGGCCGTGGCTTCGGCCAGCAATTGGCGGTCGCCACCGATGTAACGGAAGACGGTGGCTTTGGAAACGCCAGCAACACGCGCCACtt                                                                                            <  1:407948/136‑1 (MQ=255)                                                    aTAGCCGAGGCGTTCGATGACCTCTTCCAGCCGCTTGGCCGTGGCTTCGGCCAGCAATTGGCGGTCGCCACCGATGTAACGGAAGACGGTGGCTTTGGAAACGCCAGCAACACGCGCCACTTCACTGATGGTGAcc                                                                              >  1:386011/1‑136 (MQ=255)                                                    aTAGCCGAGGCGTTCGATGACCTCTTCCAGCCGCTTGGCCGTGGCTTCGGCCAGCAATTGGCGGTCGCCACCGATGTAACGGAAGACGGTGGCTTTGGAAACGCCAGCAACACGCGCCACTTCACTGATGGTGAcc                                                                              >  1:393050/1‑136 (MQ=255)                                                    aTAGCCGAGGCGTTCGATGACCTCTTCCAGCCGCTTGGCCGTGGCTTCGGCCAGCAATTGGCGGTCGCCACCGATGTAACGGAAGACGGTGGCTTTGGAAACGCCAGCAACACGCGCCACTTCACTGATGGTGAcc                                                                              >  2:514698/1‑136 (MQ=255)                                                       gcggAGGCGTTCGATGACCTCTTCCAGCCGCTTGGCCGTGGCTTCGGCCAGCAATTGGCGGTCGCCACCGATGTAACGGAAGACGGTGGCTTTGGAAACGCCAGCAACACGCGCCACTTCACTGATGGTGACCCGt                                                                           <  1:1084891/133‑1 (MQ=255)                                                       gCCGAGGCGTTCGATGACCTCTTCCAGCCGCTTGGCCGTGGCTTCGGCCAGCAATTGGCGGTCGCCACCGATGTAACGGAAGACGGTGGCTTTGGAAACGCCAGCAACACGCGCCACTTCACTGATGGTGACCCGt                                                                           <  2:875423/136‑1 (MQ=255)                                                       gCCGAGGCGTTCGATGACCTCTTCCAGCCGCTTGGCCGTGGCTTCGGCCAGCAATTGGCGGTCGCCACCGATGTAACGGAAGACGGTGGCTTTGGAAACGCCAGCAACACGCGCCACTTCACTGATGGTGACCCGt                                                                           <  2:1052816/136‑1 (MQ=255)                                                       gCCGAGGCGTTCGATGACCTCTTCCAGCCGCTTGGCCGTGGCTTCGGCCAGCAATTGGCGGTCGCCACCGATGTAACGGAAGACGGTGGCTTTGGAAACGCCAGCAACACGCGCCACTTCACTGATGGTGACCCGt                                                                           <  1:1139412/136‑1 (MQ=255)                                                       gCCGAGGCGTTCGATGACCTCTTCCAGCCGCTTGGCCGTGGCTTCGGCCAGCAATTGGCGGTCGCCACCGATGTAACGGAAGACGGTGGCTTTGGAAACGCCAGCAACACGCGCCACTTCACTGATGGTGACCCGt                                                                           <  1:270775/136‑1 (MQ=255)                                                          gAGGCGTTCGATGACCTCTTCCAGCCGCTTGGCCGTGGGTTCGGCCAGCAATTGGCGGTCGCCACCGATGTAACGGAAGACGGTGGCTTTGGAAACGCCAGCAACACGCGCCACTTCACTGATGGTGAACCGTTcg                                                                        <  2:971346/136‑1 (MQ=255)                                                                     tGACCTCTTCCAGCCGCTTGGCCGTGGCTTCGGCCAGCAATTGGCGGTCGCCACCGATGTAACGGAAGACGGTGGCTTTGGAAACGCCAGCAACACGCGCCACTTCACTGATGGTGACCCGTTCGCGGGTATGGGc                                                             >  1:1107093/1‑136 (MQ=255)                                                                         ctctTCCAGCCGCTTGGCCGTGGCTTCGGCCAGCAATTGGCGGTCGCCACCGATGTAACGGAAGACGGTGGCTTTGGAAACGCCAGCAACACGCGCCACTTCACTGATGGTGACCCGTTCGCGGGTATGGGCAGgc                                                         <  2:940738/136‑1 (MQ=255)                                                                          tctTCCAGCCGCTTGGCCGTGGCTTCGGCCAGCAATTGGCGGTCGCCACCGATGTAACGGAAGACGGTGGCTTTGGAAACGCCAGCAACACGCGCCACTTCACTGATGGTGACCCGTTCGCGGGTATGGGCAGgcg                                                        <  2:970755/136‑1 (MQ=255)                                                                              ccAGCCGCTTGGCCGTGGCTTCGGCCAGCAATTGGCGGTCGCCACCGATGTAACGGAAGACGGTGGCTTTGGAAACGCCAGCAGCACGCGCCACTTCACTGATGGTGACCCGTTCGCGGGTATGGGCAGgc                                                         >  2:263216/1‑131 (MQ=255)                                                                              ccAGCCGCTTGGCCGTGGCTTCGGCCAGCAATTGGCGGTCGCCACCGATGTAACGGAAGACGGTGGCTTTGGAAACGCCAGCAGCACGCGCCACTTCACTGATGGTGACCCGTTCGCGGGTATGGGCAGgc                                                         <  1:263216/131‑1 (MQ=255)                                                                                                 ttCGGCCAGCAATTGGCGGTCGCCACCGATGTAACGGAAGACGGTGGCTTTGGAAACGCCAGCAACACGCGCCACTTCACTGATGGTGACCCGTTCGCGGGTATGGGCAGGCGCGTCGGTCATGGGTTTTCCTTAt                                 <  2:1026778/136‑1 (MQ=255)                                                                                                 ttCGGCCAGCAATTGGCGGTCGCCACCGATGTAACGGAAGACGGTGGCTTTGGAAACGCCAGCAACACGCGCCACTTCACTGATGGTGACCCGTTCGCGGGTATGGGCAGGCGCGTCGGTCATGGGTTTTCCTTAt                                 <  1:1252195/136‑1 (MQ=255)                                                                                                 ttCGGCCAGCAATTGGCGGTCGCCACCGATGTAACGGAAGACGGTGGCTTTGGAAACGCCAGCAACACGCGCCACTTCACTGATGGTGACCCGTTCGCGGGTATGGGCAGGCGCGTCGGTCATGGGTTTTCCTTAt                                 <  2:887692/136‑1 (MQ=255)                                                                                                 ttCGGCCAGCAATTGGCGGTCGCCACCGATGTAACGGAAGACGGTGGCTTTGGAAACGCCAGCAACACGCGCCACTTCACTGATGGTGACCCGTTCGCGGGTATGGGCAGGCGCGTCGGTCATGGGTTTTCCTTAt                                 <  1:327118/136‑1 (MQ=255)                                                                                                             ttGGCGGTCGCCACCGCTGTAACGGAAGACGGTGGCTTTGGAAACGCCAGCAACACGCGCCACTTCACTGATGGTGACCCGTTCGCGGGTATGGGCAGGCGCGTCGGTCATGGGTTTTCCTTATGATTGTTTTAAt                     >  1:557461/1‑136 (MQ=255)                                                                                                             ttGGCGGTCGCCACCGATGTAACGGAAGACGGTGGCTTTGGAAACGCCAGCAACACGCGCCACTTCACTGATGGTGACCCGTTCGCGGGTATGGGCAGGCGCGTCGGTCATGGGTTTTCCTTATGATTGTTTTAAt                     >  2:54342/1‑136 (MQ=255)                                                                                                                gCGGTCGCCACCGATGTAACGGAAGAGGGTGGCTTTGGAAACGCCAGCAACACGCGCCACTTCACTGATGGTGACCCGTTCGCGGGTATGGGCAGGCGCGTCGGTCATGGGTTTTCCTTATGATTGTTTTAATAGt                  <  1:1284560/136‑1 (MQ=255)                                                                                                                      gCCACCGATGTAACGGAAGACGGTGGCTTTGGAAACGCCAGCAACACGCGCCACTTCACTGATGGTGACCCGTTCGCGGGTATGGGCAGGCGCGTCGGTCATGGGTTTTCCTTATGATTGTTTTAATAGTGATGaa            <  1:915176/136‑1 (MQ=255)                                                                                                                       ccACCGATGTAACGGAAGACGGTGGCTTTGGAAACGCCAGCAACACGCGCCACTTCACTGATGGTGACCCGTTCGCGGGTATGGGCAGGCGCGTCGGTCATGGGTTTTCCTTATGATTGTTTTAATAGTGATGaaa           >  1:900621/1‑136 (MQ=255)                                                                                                                       ccACCGATGTAACGGAAGACGGTGGCTTTGGAAACGCCAGCAACACGCGCCACTTCACTGATGGTGACCCGTTCGCGGGTATGGGCAGGCGCGTCGGTCATGGGTTTTCCTTATGATTGTTTTAATAGTGATGaaa           >  1:1076157/1‑136 (MQ=255)                                                                                                                              tGTAACGGAAGACGGTGGCTTTGGAAACGCCAGCAACACGCGCCACTTCACTGATGGTGACCCGTTCGCGTGTATGGGCAGGCGCGTCGGTCATGGGTTTTCCTTATGATTGTTTTAATAGTGATGAAACCGGttt    <  2:348337/136‑1 (MQ=255)                                                                                                                              tGTAACGGAAGACGGTGGCTTTGGAAACGCCAGCAACACGCGCCACTTCACTGATGGTGACCCGTTCGCGGGTATGGGCAGGCGCGTCGGTCATGGGTTTTCCTTATGATCGTTTTAATAGTGATGAAACCGGttt    <  2:509320/136‑1 (MQ=255)                                                                                                                                tAACGGAAGACGGTGGCTTTGGAAACGCCAGCAACACGCTCCACTTCACTGATGGTGACCCGTTCGCGGGTATGGGCAGGCGCGTCGGTCATGGGTTTTCCTTATGATTGTTTTAATAGTGATGAAACCGGTTTCa  <  2:250115/136‑1 (MQ=255)                                                                                                                                      |                                                                                                                                  GATCAGTCGCGTCTGGCCGCGCTTTAGGCCGCGAGCCATCTGGTTGGGGCGATAGCCGAGGCGTTCGATGACCTCTTCCAGCCGCTTGGCCGTGGCTTCGGCCAGCAATTGGCGGTCGCCACCGATGTAACGGGAGACGGTGGCTTTGGAAACGCCAGCAACACGCGCCACTTCACTGATGGTGACCCGTTCGCGGGTATGGGCAGGCGCGTCGGTCATGGGTTTTCCTTATGATTGTTTTAATAGTGATGAAACCGGTTTCA  >  NC_002947/3827792‑3828054

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑