Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_002947	3,942,797	G→A	S123S (AGC→AGT)	PP_3475 ←	type II secretion system pseudopilin PulG‑like signal peptide protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	3,942,797	0	G	A	100.0%	290.0 / NA	84	S123S (AGC→AGT)	PP_3475	type II secretion system pseudopilin PulG‑like signal peptide protein
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (47/37);  total (47/37)

TCTGCACACCGCGTGCACTTTCGGTAAAGGCCTGCTCGGCGACGAACTGCCAGTCGCTGTAGTGCTCCATCCCCTCGAACCCGCTCCATTCGGCGCTGAAGCCACTGCGCTTGAAAGGGGTGGCGTCCGAACGGCTGTACACTCCGGTGATACGGCCATCGATCGAGCGCAGCAGGCCCCATTCGTGGCTGTTGCTGATCGGGTCAGGGTAAAGCCTGCGAATGTGCCGTTTGGCCTGCGGGTAGCGGTTGTCCTGTAGCAGGTC  >  NC_002947/3942664‑3942928                                                                                                                                      |                                                                                                                                    tCTGCACACCGCGTGCACTTTCGGTAAAGGCCTGCTCGGCGACGAACTGCCAGTCGCTGTAGTGCTCCATCCCCTCGAACCCGCTCCATTCGGCGCTGAAGCCACTGCGCTTGAAAGGGGGGGCGTCCGAACGACt                                                                                                                                   <  1:14093/136‑1 (MQ=255)   tGCACACCGCGTGCACTTTCGGTAAAGGCCTGCTCGGCGACGAACTGCCAGTCGCTGTAGTGCTCCATCCCCTCGAACCCGCTCCATTCGGCGCTGAAGCCACTGCGCTTGAAAGGGGTGGCGTCCGAACGACTGt                                                                                                                                 >  1:24067/1‑136 (MQ=255)    gCACACCGCGTGCACTTTCGGTAAAGGCCTGCTCGGCGACGAACTGCCAGTCGCTGTAGTGCTCCATCCCCTCGACCCCGCTCCATTCGGCGCTGAAGCCACTGCGCTTGAAAGGGGTGGCGTCCGAACGACTGTa                                                                                                                                <  2:704660/136‑1 (MQ=255)    gCACACCGCGTGCACTTTCGGTAAAGGCCTGCTCGGCGACGAACTGCCAGTCGCTGTAGTGCTCCATCCCCTCGAACCCGCTCCATTCGGCGCTGAAGCCACTGCGCTTGAAAGGGGTGGCGTCCGAACGACTGTa                                                                                                                                >  2:842827/1‑136 (MQ=255)        acCGCGTGCACTTTCGGTAAAGGCCTGCTCGGCGACGAACTGCCAGTCGCTGTAGTGCTCCATCCCCTCGAACCCGCTCCATTCGGCGCTGAAGCCACTGCGCTTGAAAGGGGTGGCGTCCGAACGACTGTACACt                                                                                                                            >  2:853680/1‑136 (MQ=255)        acCGCGTGCACTTTCGGTAAAGGCCTGCTCGGCGACGAACTGCCAGTCGCTGTAGTGCTCCATCCCCTCGAACCCGCTCCATTCGGCGCTGAAGCCACTGCGCTTGAAAGGGGTGGCGTCCGAACGACTGTACACt                                                                                                                            <  1:859036/136‑1 (MQ=255)        acCGCGTGCACTTTCGGTAAAGGCCTGCTCGGCGACGAACTGCCAGTCGCTGTAGTGCTCCATCCCCTCGAACCCGCTCCATTCGGCGCTGAAGCCACTGCGCTTGAAAGGGGTGGCGTCCGAACGACTGTACACt                                                                                                                            >  2:291519/1‑136 (MQ=255)        acCGCGTGCACTTTCGGTAAAGGCCTGCTCGGCGACGAACTGCCAGTCGCTGTAGTGCTCCATCCCCTCGAACCCGCTCCATTCGGCGCTGAAGCCACTGCGCTTGAAAGGGGTGGCGTCCGAACGACTGTACACt                                                                                                                            >  2:335365/1‑136 (MQ=255)            cgTGCACTTTCGGTAAAGGCCTGCTCGGCGACGAACTGCCAGTCGCTGTAGTGCTCCATCCCCTCGAACCCGCTCCATTCGGCGCTGAAGCCACTGCGCTTGAAAGGGGTGGCGTCCGAACGACTGTACACTCCgg                                                                                                                        <  1:1361176/136‑1 (MQ=255)             gTGCACTTTCGGTAAAGGCCTGCTCGGCGACGAACTGCCAGTCGCTGTAGTGCTCCATCCCCTCGAACCCGCTCCATTCGGCGCTGAAGCCACTGCGCTTGAAAGGGGTGGCGTCCGAACGACTGTACACTCCGGt                                                                                                                       >  2:612440/1‑136 (MQ=255)             gTGCACTTTCGGTAAAGGCCTGCTCGGCGACGAACTGCCAGTCGCTGTAGTGCTCCATCCCCTCGAACCCGCTCCATTCGGCGCTGAAGCCACTGCGCTTGAAAGGGGTGGCGTCCGAACGACTGTACACTCCGGt                                                                                                                       >  1:453890/1‑136 (MQ=255)              tGCACTTTCGGTAAAGGCCTGCTCGGCGACGAACTGCCAGTCGCTGTAGTGCTCCATCCCCTCGAACCCGCTCCATTCGGCGCTGAAGCCACTGCGCTTGAAAGGGGTGGCGTCCGAACGACTGTACACTCCGGTg                                                                                                                      >  2:1213488/1‑136 (MQ=255)              tGCACTTTCGGTAAAGGCCTGCTCGGCGACGAACTGCCAGTCGCTGTAGTGCTCCATCCCCTCGAACCCGCTCCATTCGGCGCTGAAGCCACTGCGCTTGAAAGGGGTGGCGTCCGAACGACTGTACACTCCGGTg                                                                                                                      <  1:335365/136‑1 (MQ=255)                cACTTTCGGTAAAGGCCTGCTCGGCGACGAACTGCCAGTCGCTGTAGTGCTCCATCCCCTCGAACCCGCTCCATTCGGCGCTGATGCCACTGCGCTTGAAAGGGGTGGCGTCCGAACGACTGTACACTCCGGTGAt                                                                                                                    <  1:1109432/136‑1 (MQ=255)                cACTTTCGGTAAAGGCCTGCTCGGCGACGAACTGCCAGTCGCTGTAGTGCTCCATCCCCTCGAACCCGCTCCATTCGGCGCTGAAGCCACTGCGCTTGAAAGGGGTGGCGTCCGAACGACTGTACACTCCGGTGAt                                                                                                                    <  1:1255746/136‑1 (MQ=255)                cACTTTCGGTAAAGGCCTGCTCGGCGACGAACTGCCAGTCGCTGTAGTGCTCCATCCCCTCGAACCCGCTCCATTCGGCGCTGAAGCCACTGCGCTTGAAAGGGGTGGCGTCCGAACGACTGTACACTCCGGTGAt                                                                                                                    <  1:294061/136‑1 (MQ=255)                             ggCCTGCTCGGCGACGAACTGCCAGTCGCTGTAGTGCTCCATCCCCTCGAACCCGCTCCATTCGGCGCTGAAGCCACTGCGCTTGAAAGGGGTGGCGTCCGAACGACTGTACACTCCGGTGATACGGCCatcgatc                                                                                                       <  2:453890/136‑1 (MQ=255)                             ggCCTGCTCGGCGACGAACTGCCAGTCGCTGTAGTGCTCCATCCCCTCGAACCCGCTCCATTCGGCGCTGAAGCCACTGCGCTTGAAAGGGGTGGCGTCCGAACGACTGTACACTCCGGTGATACGGCCatcgatc                                                                                                       <  2:740231/136‑1 (MQ=255)                             ggCCTGCTCGGCGACGAACTGCCAGTCGCTGTAGTGCTCCATCCCCTCGAACCCGCTCCATTCGGCGCTGAAGCCACTGCGCTTGAAAGGGGTGGCGTCCGAACGACTGTACACTCCGGTGATACGGCCatcgatc                                                                                                       <  2:768586/136‑1 (MQ=255)                                cTGCTCGGCGACGAACTGCCAGTCGCTGTAGTGCTCCATCCCCTCGAACCCGCTCCATTCGGCGCTGAAGCCACTGCGCTTGAAAGGGGTGGCGTCCGAACGACTGTACACTCCGGTGATACGGCCATCGATCGAg                                                                                                    >  2:1216619/1‑136 (MQ=255)                                cTGCTCGGCGACGAACTGCCAGTCGCTGTAGTGCTCCATCCCCTCGAACCCGCTCCATTCGGCGCTGAAGCCACTGCGCTTGAAAGGGGTGGCGTCCGAACGACTGTACACTCCGGTGATACGGCCATCGATCGAg                                                                                                    >  2:9864/1‑136 (MQ=255)                                   cTCGGCGACGAACTGCCAGTCGCTGTAGTGCTCCATCCCCTCGAACCCGCTCCATTCGGCGCTGAAGCCACTGCGCTTGAAAGGGGTGGCGTCCGAACGACTGTACACTCCGGTGATACGGCCATCGATCGAgcgc                                                                                                 <  1:905540/136‑1 (MQ=255)                                    tCGGCGACGAACTGCCAGTCGCTGTAGTGCTCCATCCCCTCGAACCCGCTCCATTCGGCGCTGAAGCCACTGCGCTTGAAAGGGGTGGCGTCCGAACGACTGTACACTCCGGTGATACGGCCATCGATCGAgcgcc                                                                                                >  1:1231016/1‑135 (MQ=255)                                    tCGGCGACGAACTGCCAGTCGCTGTAGTGCTCCATCCCCTCGAACCCGCTCCATTCGGCGCTGAAGCCACTGCGCTTGAAAGGGGTGGCGTCCGAACGACTGTACACTCCGGTGATACGGCCATCGATCGAGCgca                                                                                                >  1:1255461/1‑136 (MQ=255)                                     cGGCGACGAACTGCCAGTCGCTGTAGTGCTCCATCCCCTCGAACCCGCTCCATTCGGCGCTGAAGCCACTGCGCTTGAAAGGGGTGGCGTCCGAACGACTGTACACTCCGGTGATACGGCCATCGATCGAGCgcag                                                                                               >  1:849382/1‑136 (MQ=255)                                                     gTCGCTGTAGTGCTCCATCCCCTCGAACCCGCTCCATTCGGCGCTGAAGCCACTGCGCTTGAAAGGGGTGGCGTCCGAACGACTGTACACTCCGGTGATACGGCCATCGATCGAGCGCAGCAGGCCCCATTCGTgg                                                                               >  2:68959/1‑136 (MQ=255)                                                             tgTGCTCCATCCCCTCGAACCCGCTCCATTCGGCGCTGAAGCCACTGCGCTTGAAAGGGGTGGCGTCCGAACGACTGTACACTCCGGTGATACGGCCATCGATCGAGCGCAGCAGGCCCCATTCGTGGCTGTTGCt                                                                       <  1:853680/135‑1 (MQ=255)                                                             aGTGCTCCATCCCCTCGAACCCGCTCCATTCGGCGCTGAAGCCACTGCGCTTGAAAGGGGTGGCGTCCGAACGACTGTACACTCCGGTGATACGGCCATCGATCGAGCGCAGCAGGCCCCATTCGTGGCTGTTGCt                                                                       <  1:470730/136‑1 (MQ=255)                                                             aGTGCTCCATCCCCTCGAACCCGCTCCATTCGGCGCTGAAGCCACTGCGCTTGAAAGGGGTGGCGTCCGAACGACTGTACACTCCGGTGATACGGCCATCGATCGAGCGCAGCAGGCCCCATTCGTGGCTGTTGCt                                                                       >  1:834680/1‑136 (MQ=255)                                                               tGCTCCATCCCCTCGAACCCGCTCCATTCGGCGCTGAAGCCACTGCGCTTGAAAGGGGTGGCGTCCGAACGACTGTACACTCCGGTGATACGGCCATCGATCGAGCGCAGCAGGCCCCATTCGTGGCTGTTGCTgt                                                                     >  2:1147920/1‑135 (MQ=255)                                                               tGCTCCATCCCCTCGAACCCGCTCCATTCGGCGCTGAAGCCACTGCGCTTGAAAGGGGTGGCGTCCGAACGACTGTACACTCCGGTGATACGGCCATCGATCGAGCGCAGCAGGCCCCATTCGTGGCTGTTGCTGa                                                                     >  2:739279/1‑136 (MQ=255)                                                                  tCCATCCCCTCGAACCCGCTCCATTCGGCGCTGAAGCCACTGCGCTTGAAAGGGGTGGCGTCCGAACGACTGTACACTCCGGTGATACGGCCATCGATCGAGCGCAGCAGGCCCCATTCGTGGCTGTTGCTGATCg                                                                  <  1:1213488/136‑1 (MQ=255)                                                                  tCCATCCCCTCGAACCCGCTCCATTCGGCGCTGAAGCCACTGCGCTTGAAAGGGGTGGCGTCCGAACGACTGTACACTCCGGTGATACGGCCATCGATCGAGCGCAGCAGGCCCCATTCGTGGCTGTTGCTGATCg                                                                  <  1:1354055/136‑1 (MQ=255)                                                                  tCCATCCCCTCGAACCCGCTCCATTCGGCGCTGAAGCCACTGCGCTTGAAAGGGGTGGCGTCCGAACGACTGTACACTCCGGTGATACGGCCATCGATCGAGCGCAGCAGGCCCCATTCGTGGCTGTTGCTGATCg                                                                  <  2:16729/136‑1 (MQ=255)                                                                  cccATCCCCTCGAACCCGCTCCATTCGGCGCTGAAGCCACTGCGCTTGAAAGGGGTGGCGTCCGAACGACTGTACACTCCGGTGATACGGCCATCGATCGAGCGCAGCAGGCCCCATTCGTGGCTGTTGCTGATCg                                                                  <  1:336283/135‑1 (MQ=255)                                                                   ccATCCCCTCGAACCCGCTCCATTCGGCGCTGAAGCCACTGCGCTTGAAAGGGGTGGCGTCCGAACGACTGTACACTCCGGTGATACGGCCATCGATCGAGCGCAGCAGGCCCCATTCGTGGCTGTTGCTGATCgg                                                                 >  1:629008/1‑136 (MQ=255)                                                                   ccATCCCCTCGAACCCGCTCCATTCGGCGCTGAAGCCACTGCGCTTGAAAGGGGTGGCGTCCGAACGACTGTACACTCCGGTGATACGGCCATCGATCGAGCGCAGCAGGCCCCATTCGTGGCTGTTGCTGATCgg                                                                 <  1:389881/136‑1 (MQ=255)                                                                    cATCCCCTCGAACCCGCTCCATTCGGCGCTGAAGCCACTGCGCTTGAAAGGTGTGGCGTCCGAACGACTGTACACTCCCGTGATACGGCCATCGATCGAGCGCAGCAGGCCCCAATCGACGCTCTACCTGATCtgg                                                                >  2:155316/1‑136 (MQ=255)                                                                    cATCCCCTCGAACCCGCTCCATTCGGCGCTGAAGCCACTGCGCTTGAAAGGGGTGGCGTCCGAACGACTGTACACTCCGGTGATACGGCCATCGATCGAGCGCAGCAGGCCCCATTCGTGGCTGTTGCTGATCggg                                                                >  1:423163/1‑136 (MQ=255)                                                                    cATCCCCTCGAACCCGCTCCATTCGGCGCTGAAGCCACTGCCCTAGAAAGGGGTGGCGTCCGAACGACTGTACACTCCGGTGATACGGCCATCGATCGAGCGCAGCAGGCCCCATTCGTGGCTGTTGCTGATCggg                                                                >  2:163979/1‑136 (MQ=255)                                                                       ccccTCGAACCCGCTCCATTCGGCGCTGAAGCCACTGCGCTTGAAAGGGGTGGCGTCCGAACGACTGTACACTCCGGTGATACGGCCATCGATCGAGCGCAGCAGGCCCCATTCGt                                                                                 >  1:1360455/1‑116 (MQ=255)                                                                       ccccTCGAACCCGCTCCATTCGGCGCTGAAGCCACTGCGCTTGAAAGGGGTGGCGTCCGAACGACTGTACACTCCGGTGATACGGCCATCGATCGAGCGCAGCAGGCCCCATTCGt                                                                                 <  2:1360455/116‑1 (MQ=255)                                                                       ccccTCGAACCCGCTCCATTCGGCGCTGAAGCCACTGCGCTTGAAAGGGGTGGCGTCCGAACGACTGTACACTCCGGTGATACGGCCATCGATCGAGCGCAGCAGGCCCCATTCGTGGCTGTTGCTGATCGGGTCa                                                             >  2:899462/1‑136 (MQ=255)                                                                         ccTCGAACCCGCTCCATTCGGCGCTGAAGCCACTGCGCTTGAAAGGGGTGGCGTCCGAACGACTGTACACTCCGGTGATACGGCCATCGATCGAGCGCAGCAGGCCCCATTCGTGGCTGTTGCTGATCgg                                                                 <  1:1025350/130‑1 (MQ=255)                                                                         ccTCGAACCCGCTCCATTCGGCGCTGAAGCCACTGCGCTTGAAAGGGGTGGCGTCCGAACGACTGTACACTCCGGTGATACGGCCATCGATCGAGCGCAGCAGGCCCCATTCGTGGCTGTTGCTGATCgg                                                                 >  2:1025350/1‑130 (MQ=255)                                                                         ccTCGAACCCGCTCCATTCGGCGCTGAAGCCACTGCGCTTGAAAGGGGTGGCGTCCGAACGACTGTACACTCCGGTGATACGGCCATCGATCGAGCGCAGCAGGCCCCATTCGTGGCTGTTGCTGATCGGGTCAgg                                                           >  1:1073626/1‑136 (MQ=255)                                                                         ccTCGAACCCGCTCCATTCGGCGCTGAAGCCACTGCGCTTGAAAGGGGTGGCGTCCGAACGACTGTACACTCCGGTGATACGGCCATCGATCGAGCGCAGCAGGCCCCATTCGTGGCTGTTGCTGATCGGGTCAgg                                                           >  1:59994/1‑136 (MQ=255)                                                                         ccTCGAACCCGCTCCATTCGGCGCTGAAGCCACTGCGCTTGAAAGGGGTGGCGTCCGAACGACTGTACACTCCGGTGATACGGCCATCGATCGAGCGCAGCAGGCCCCATTCGTGGCTGTTGCTGATCGGGTCAgg                                                           >  1:994592/1‑136 (MQ=255)                                                                         ccTCGAACCCGCTCCATTCGGCGCTGAAGCCACTGCGCTTGAAAGGGGTGGCGTCCGAACGACTGTACACTCCGGTGATACGGCCATCGATCGAGCGCAGCAGGCCCCATTCGTGGCTGTTGCTGATCGGGTCAgg                                                           >  1:628208/1‑136 (MQ=255)                                                                            cGAACCCGCTCCATTCGGCGCTGAAGCCACTGCGCTTGAAAGGGGTGGCGTCCGAACGACTGTACACTCCGGTGATACGGCCATCGATCGAGCGCAGCAGGCCCCATTCGTGGCTGTTGCTGATCGGGTCAGGGTa                                                        >  2:1133036/1‑136 (MQ=255)                                                                             gAACCCGCTCCATTCGGCGCTGAAGCCACTGCGCTTGAAAGGGGTGGCGTCCGAACGACTGTACACTCCGGTGATACGGCCATCGATCGAGCGCAGCAGGCCCCATTCGTGGCTGTTGCTGATCGGGTCAGGGTaa                                                       >  2:702837/1‑136 (MQ=255)                                                                              aaCCCGCTCCATTCGGCGCTGAAGCCACTGCGCTTGAAAGGGGTGGCGTCCGAACGACTGTACACTCCGGTGATACGGCCATCGATCGAGCGCAGCAGGCCCCATTCGTGGCTGTTGCTGATCGGGTCAGGGTaaa                                                      >  1:1073728/1‑136 (MQ=255)                                                                              aaCCCGCTCCATTCGGCGCTGAAGCCACTGCGCTTGAAAGGGGTGGCGTCCGAACGACTGTACACTCCGGTGATACGGCCATCGATCGAGCGCAGCAGGCCCCATTCGTGGCTGTTGCTGATCGGGTCAGGGTaaa                                                      >  2:621053/1‑136 (MQ=255)                                                                              aaCCCGCTCCATTCGGCGCTGAAGCCACTGCGCTTGAAAGGGGTGGCGTCCGAACGACTGTACACTCCGGAGATACGGCCATCGAACGAGCGCAGCAGGCCCCATTCGTGTCTGTTGCTGATCGGCTCAGAGTaaa                                                      >  2:794617/1‑136 (MQ=255)                                                                               ccccGCTCCATTCGGCGCTGAAGCCACTGCGCTTGAAAGGGGTGGCGTCCGAACGACTGTACACTCCGGTGATACGGCCATCGATCGAGCGCAGCAGGCCCCATTCGTGGCTGTTGCTGATCGGGTCAGGGTAAAg                                                     <  1:253642/135‑1 (MQ=255)                                                                                cccGCTCCATTCGGCGCTGAAGCCACTGCGCTTGAAAGGGGTGGCGTCCGAACGACTGTACACTCCGGTGATACGGCCATCGATCGAGCGCAGCAGGCCCCATTCGTGGCTGTTGCTGATCGGGTCAGGGTAAAGc                                                    <  1:67296/136‑1 (MQ=255)                                                                                      ccATTCGGCGCTGAAGCCACTGCGCTTGAAAGGGGTGGCGTCCGAACGACTGTACACTCCGGTGATACGGCCATCGATCGAGCGCAGCAGGCCCCATTCGTGGCTGTTGCTGATCGGGTCAg                                                            <  1:1229939/122‑1 (MQ=255)                                                                                      ccATTCGGCGCTGAAGCCACTGCGCTTGAAAGGGGTGGCGTCCGAACGACTGTACACTCCGGTGATACGGCCATCGATCGAGCGCAGCAGGCCCCATTCGTGGCTGTTGCTGATCGGGTCAg                                                            >  2:1229939/1‑122 (MQ=255)                                                                                       cATTCGGCGCTGATGCCACTGCGCTTGAAAGGGGTGGCGTCCGAACGACTGTACACTCCGGTGATACGGCCATCGATCGAGCGCAGCAGGCCCCATTCGTGGCTGTTGCTGATCGGGTCAGGGTAAAGCCTGCGaa                                             <  1:568028/136‑1 (MQ=255)                                                                                       cATTCGGCGCTGAAGCCACTGCGCTTGAAAGGGGTGGCGTCCGAACGACTGTACACTCCGGTGATACGGCCATCGATCGAGCGCAGCAGGCCCCATTCGTGGCTGTTGCTGATCGGGTCAGGGTAAAGCCTGCGaa                                             <  1:413287/136‑1 (MQ=255)                                                                                       cATTCGGCGCTGAAGCCACTGCGCTTGAAAGGGGTGGCGTCCGAACGACTGTACACTCCGGTGATACGGCCATCGATCGAGCGCAGCAGGCCCCATTCGTGGCTGTTGCTGATCGGGTCAGGGTAAAGCCTGCGaa                                             >  1:1101079/1‑136 (MQ=255)                                                                                       cATTCGGCGCTGAAGCCACTGCGCTTGAAAGGGGTGGCGTCCGAACGACTGTACACTCCGGTGATACGGCCATCGATCGAGCGCAGCAGGCCCCATTCGTGGCTGTTGCTGATCGGGTCAGGGTAAAGCCTGCGaa                                             <  1:561086/136‑1 (MQ=255)                                                                                            ggCGCTGAAGCCACTGCGCTTGAAAGGGGTGGCGTCCGAACGACTGTACACTCCGGTGATACGGCCATCGATCGAGCGCAGCAGGCCCCATTCGTGGCTGTTGCTGATCGGGTCAGGGTAAAGCCTGCGAATGTGc                                        >  2:480371/1‑136 (MQ=255)                                                                                             gcgcTGAAGCCACTGCGCTTGAAAGGGGTGGCGTCCGAACGACTGTACACTCCGGTGATACGGCCATCGATCGAGCGCAGCAGGCCCCATTCGTGGCTGTTGCTGATCGGGTCAGGGTAAAGCCTGCGAATGTGcc                                       <  1:886466/136‑1 (MQ=255)                                                                                             gcgcGGAAGCCACTGCGCTTGAAAGGGGTGGCGTCCGAACGACTGTACACTCCGGTGATACGGCCATCGATCGAGCGCAGCAGGCCCCATTCGTGGCTGTTGCTGATCGGGTCAGGGTAAAGCCTGCGAATGTGcc                                       <  1:419375/136‑1 (MQ=255)                                                                                                 tGAAGCCACTGCGCTTGAAAGGGGTGGCGTCCGAACGACTGTACACTCCGGTGATACGGCCATCGATCGAGCGCAGCAGGCCCCATTCGTGGCTGTTGCTGATCGGGTCAGGGTAAAGCCTGCGAATGTGCCGttt                                   >  2:1146096/1‑136 (MQ=255)                                                                                                  gAAGCCACTGCGCTTGAAAGGGGTGGCGTCCGAACGACTGTACACTCCGGTGATACGGCCATCGATCGAGCGCAGCAGGCCCCATTCGTGGCTGTTGCTGATCGGGTCAGGGTAAAGCCTGCGAATGTGCCGTTTg                                  >  2:815814/1‑136 (MQ=255)                                                                                                   aaGCCACTGCGCTTGAAAGGGGTGGCGTCCGAACGACTGTACACTCCGGTGATACGGCCATCGATCGAGCGCAGCAGGCCCCATTCGTGGCTGTTGCTGATCGGGTCAGGGTAAAGCCTGCGAATGTGCCGTTTgg                                 <  2:348120/136‑1 (MQ=255)                                                                                                    aGCCACTGCGCTTGAAAGGGGTGGCGTCCGAACGACTGTACACTCCGGTGATACGGCCATCGATCGAGCGCAGCAGGCCCCATTCGTGGCTGTTGCTGATCGGGTCAGGGTAAAGCCTGCGAATGTGCCGTTTGGc                                >  2:287963/1‑136 (MQ=255)                                                                                                    aGCCACTGCGCTTGAAAGGGGTGGCGTCCGAACGACTGTACACTCCGGTGATACGGCCATCGATCGAGCGCAGCAGGCCCCATTCGTGGCTGTTGCTGATCGGGTCAGGGTAAAGCCTGCGAATGTGCCGTTTGGc                                >  1:928987/1‑136 (MQ=255)                                                                                                    aGCCACTGCGCTTGAAAGGGGTGGCGTCCGAACGACTGTACACTCCGGTGATACGGCCATCGATCGAGCGCAGCAGGCCCCATTCGTGGCTGTTGCTGATCGGGTCAGGGTAAAGCCTGCGAATGTGCCGTTTGGc                                >  2:235156/1‑136 (MQ=255)                                                                                                           gcgcTTGAAAGGGGTGGCGTCCGAACGACTGTACACTCCGGTGATACGGCCATCGATCGAGCGCAGCAGGCCCCATTCGTGGCTGTTGCTGATCGGGTCAGGGTAAAGCCTGCGAATGTGCCGTTTGGCCTGCggg                         >  1:398266/1‑136 (MQ=255)                                                                                                             gcTTGAAAGGGGTGGCGTCCGAACGACTGTACACTCCGGTGATACGGCCATCGATCGAGCGCAGCAGGCCCCATTCGTGGCTGTTGCTGATCGGGTCAGGGTAAAGCCTGCGAATGTGCCGTTTGGCCTGCGGGTa                       >  2:1340515/1‑136 (MQ=255)                                                                                                              cTTGAAAGGGGTGGCGTCCGAACGACTGTACACTCCGGTGATACGGCCATCGATCGAGCGCAGCAGGCCCCATTCGTGGCTGTTGCTGATCGGGTCAGGGTAAAGCCTGCGAATGTGCCGTTTGGCCTGCGGGTAg                      >  2:737691/1‑136 (MQ=255)                                                                                                              cTTGAAAGGGGTGGCGTCCGAACGACTGTACACTCCGGTGATACGGCCATCGATCGAGCGCAGCAGGCCCCATTCGTGGCTGTTGCTGATCGGGTCAGGGTAAAGCCTGCGAATGTGCCGTTTGGCCTGCGGGTAg                      <  2:628208/136‑1 (MQ=255)                                                                                                              cTTGAAAGGGGTGGCGTCCGAACGACTGTACACTCCGGTGATACGGCCATCGATCGAGCGCAGCAGGCCCCATTCGTGGCTGTTGCTGATCGGGTCAGGGTAAAGCCTGCGAATGTGCCGTTTGGCCTGCGGGTAg                      <  2:59994/136‑1 (MQ=255)                                                                                                               ttGAAAGGGGTGGCGTCCGAACGACTGTACACTCCGGTGATACGGCCATCGATCGAGCGCAGCAGGCCCCATTCGTGGCTGTTGCTGATCGGGTCAGGGTAAAGCCTGCGAATGTGCCGTTTGGCCTGCGGGTAGc                     <  1:155316/136‑1 (MQ=255)                                                                                                                   aaGGGGTGGCGTCCGAACGACTGTACACTCCGGTGATACGGCCATCGATCGAGCGCAGCAGGCCCCATTCGTGGCTGTTGCTGATCGGGTCAGGGTAAAGCCTGCGAATGTGCCGTTTGGCCTGCGGGTAGCGGtt                 <  2:1196370/136‑1 (MQ=255)                                                                                                                   aaGGGGTGGCGTCCGAACGACTGTACACTCCGGTGATACGGCCATCGATCGAGCGCAGCAGGCCCCATTCGTGGCTGTTGCTGATCGGGTCAGGGTAAAGCCTGCGAATGTGCCGTTTGGCCTGCGGGTAGCGGtt                 <  2:846365/136‑1 (MQ=255)                                                                                                                           gCGTCCGAACGACTGTACACTCCGGTGATACGGCCATCGATCGAGCGCAGCAGGCCCCATTCGTGGCTGTTGCTGATCGGGTCAGGGTAAAGCCTGCGAATGTGCCGTTTGGCCTGCGGGTAGCGGTTGTCCTGTa         >  2:526269/1‑136 (MQ=255)                                                                                                                            cGTCCGAACGACTGTACACTCCGGTGATACGGCCatcgatc                                                                                                       <  2:1024234/41‑1 (MQ=255)                                                                                                                            cGTCCGAACGACTGTACACTCCGGTGATACGGCCatcgatc                                                                                                       >  1:1024234/1‑41 (MQ=255)                                                                                                                            cGTCCGAACGACTGTACACTCCGGTGATACGGCCATCGATCGAGCGCAGCAGGCCCCATTCGTGGCTGTTGCTGATCGGGTCAGGGTAAAGCCTGCGAATGTGCCGTTTGGCCTGCGGGTAGCGGTTGTCCTGTAg        >  2:242956/1‑136 (MQ=255)                                                                                                                                  aaCGACTGTACACTCCGGTGATACGGCCATCGATCGAGCGCAGCAGGCCCCATTCGTGGCTGTTGCTGATCGGGTCAGGGTAAAGCCTGCGAATGTGCCGTTTGGCCTGCGGGTAGCGGTTGTCCTGTAGCAGGTc  <  1:818255/136‑1 (MQ=255)                                                                                                                                      |                                                                                                                                    TCTGCACACCGCGTGCACTTTCGGTAAAGGCCTGCTCGGCGACGAACTGCCAGTCGCTGTAGTGCTCCATCCCCTCGAACCCGCTCCATTCGGCGCTGAAGCCACTGCGCTTGAAAGGGGTGGCGTCCGAACGGCTGTACACTCCGGTGATACGGCCATCGATCGAGCGCAGCAGGCCCCATTCGTGGCTGTTGCTGATCGGGTCAGGGTAAAGCCTGCGAATGTGCCGTTTGGCCTGCGGGTAGCGGTTGTCCTGTAGCAGGTC  >  NC_002947/3942664‑3942928

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑