Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_002947	963,645	C→T	A84V (GCG→GTG)	phnC →	phosphonate ABC transporter ATP‑binding protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	963,645	0	C	T	100.0%	190.0 / NA	55	A84V (GCG→GTG)	phnC	phosphonate ABC transporter ATP‑binding protein
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (35/20);  total (35/20)

GGGCCGTCGGGGGCGGGCAAGTCCAGCTTGCTGCACCTGATGGCCACGGCCATCCAGCCCAGCAGTGGGCGCCTGGAGTTGCTTGGCGAGCAGCCTTGGGCACTGTCGGCAAGGGCACGCCAGCGACTGCGTGCGCGGGTTGGCCTGGTGCATCAGTCGCCGCCTTTGCCACCGCGTCAGCGGGTGGTGACTGCCGTACTGGCGGGTCGCCTGGGGCAGTGGGGGACGCTGCGCGGCTTGCTCAACCTGCTGTATCCCAGCG  >  NC_002947/963512‑963773                                                                                                                                      |                                                                                                                                 gggCCGTCGGGGGCGGGCAAGTCCAGCTTGCTGCACCTGATGGCCACGGCCATCCAGCCCAGCAGTGGGCGCCTGGAGTTGCTTGGCGAGCAGCCTTGGGCACTGTCGGCAAGGGCACGCCAGCGACTGCGTgtgc                                                                                                                                <  2:1274512/136‑1 (MQ=255)     cGTCGGGGGCGGGCAAGTCCAGCTTGCTGCACCTGATGGCCACGGCCATCCAGCCCAGCAGTGGGCGCCTGGAGTTGCTTGGCGAGCAGCCTTGGGCACTGTCGGCAAGGGCACGCCAGCGACTGCGTGTGCGGGt                                                                                                                            >  2:957639/1‑136 (MQ=255)         gggggCGGGCAAGTCCAGGTGGGTTCCCGTGTTGCCCACGGCCATCCAGCCCTGCAGTGGGCGCCTGGAGTTGCTTGGCGAGCAGCCTTGGGCACTGTCGGCAAGGGCACGCCAGCGACTGCGTGTGCGGGTTGGc                                                                                                                        <  2:1164138/136‑1 (MQ=255)                         aGCTTGCTGCACCTGATGGCCACGGCCATCCAGCCCAGCAGTGGGCGCCTGGAGTTGCTTGGCGAGCAGCCTTGGGCACTGTCGGCAAGGGCACGCCAGCGACTGCGTGTGCGGGTTGGCCTGGTGCATCAGTcgc                                                                                                        >  1:660119/1‑136 (MQ=255)                             tgctgcACCTGATGGCCACGGCCATCCAGCCCAGCAGTGGGCGCCTGGAGTTGCTTGGCGAGCAGCCTTGGGCACTGTCGGCAAGGGCACGCCAGCGACTGCGTGTGCGGGTTGGCCTGGTGCATCAGTCgccgcc                                                                                                    >  2:657061/1‑136 (MQ=255)                                        aTGGCCACGGCCATCCAGCCCAGCAGTGGGCGCCTGGAGTTGCTTGGCGAGCAGCCATGGGCACTGTCGGCAAGGGCACGCCAGCGACTGCGTGTGCGGGTTGGCCTGGTGCATCAGTCGCCGGCAATGCCACcgc                                                                                         >  2:669764/1‑136 (MQ=255)                                          ggCCACGGCCATCCAGCCCAGCAGTGGGCGCCTGGAGTTGCTTGGCGAGCAGCCTTGGGCACTGTCGGCAAGGGCACGCCAGCGACTGCGTGTGCGGGTTGGCCTGGTGCATCAGTCGCCGCCTTTGCCACcgcgg                                                                                       >  2:485061/1‑135 (MQ=255)                                          ggCCACGGCCATCCAGCCCAGCAGTGGGCGCCTGGAGTTGCTTGGCGAGCAGCCTTGGGCACTGTCGGCAAGGGCACGCCAGCGACTGCGTGTGCGGGTTGGCCTGGTGCATCAGTCGCCGCCTTTGCCACCGCGt                                                                                       <  2:267810/136‑1 (MQ=255)                                           gCCACGGCCATCCAGCCCATCAGTGGGCGCCTGGAGTTGCTTGGCGAGCAGCCTTGGGCACTGTCGGCAAGGGCACGCCAGCGACTGCGTGTGCGGGTTGGCCTGGTGCATCAGTCGCCGCCTTTGCCAGCGCGTc                                                                                      <  2:1135354/136‑1 (MQ=255)                                             cACGGCCATCCAGCCCAGCAGTGGGCGCCTGGAGTTGCTTGGCGAGCAGCCTTGGGCACTGTCGGCAAGGGCACGCCAGCGACTGCGTGTGCGGGTTGGCCTGGTGCATCAGTCGCCGCCTTTGCCACCGCGTCAg                                                                                    <  1:82801/136‑1 (MQ=255)                                                  ccATCCAGCCCAGCAGTGGGCGCCTGGAGTTGCTTGGCGAGCAGCCTTGGGCACTGTCGGCAAGGGCACGCCAGCGACTGCGTGTGCGGGTTGGCCTGGTGCATCAGTCGCCGCCTTTGCCACCGCGTCAGCGggt                                                                               >  2:541539/1‑136 (MQ=255)                                                  ccATCCAGCCCAGCAGTGGGCGCCTGGAGTTGCTTGGCGAGCAGCCTTGGGCACTGTCGGCAAGGGCACGCCAGCGACTGCGTGTGCGGGTTGGCCAGGTGCATCAGTCGCCCCCTTTTCCACCCCGTCAGCGggt                                                                               >  2:548682/1‑136 (MQ=255)                                                        agcccagcAGTGGGCGCCTGGAGTTGCTTGGCGAGCAGCCTTGGGCACTGTCGGCAAGGGCACGCCAGCGACTGCGTGTGCGGGTTGGCCTGGTGCATCAGTCGCCGCCTTTGCCACCGCGTCAGCGGGTGGTGAc                                                                         >  2:29987/1‑136 (MQ=255)                                                             agcagTGGGCGCCTGGAGTTGCTTGGCGAGCAGCCTTGGGCACTGTCGGCAAGGGCACGCCAGCGACTGCGTGTGCGGGTTGGCCTGGTGCATCAGTCGCCGCCTTTGCCACCGCGTCAGCGGGTGGTGACTGCCg                                                                    >  2:136861/1‑136 (MQ=255)                                                             agcagTGGGCGCCTGGAGTTGCTTGGCGAGCAGCCTTGGGCACTGTCGGCAAGGGCACGCCAGCGACTGCGTGTGCGGGTTGGCCTGGTGCATCAGTCGCCGCCTTTGCCACCGCGTCAGCGGGTGGTGACTGCCg                                                                    >  1:968733/1‑136 (MQ=255)                                                             agcagTGGGCGCCTGGAGTTGCTTGGCGAGCAGCCTTGGGCACTGTCGGCAAGGGCACGCCAGCGACTGCGTGTGCGGGTTGGCCTGGTGCATCAGTCGCCGCCTTTGCCACCGCGTCAGCGGCTGGTGACTGCCg                                                                    >  1:305840/1‑136 (MQ=255)                                                                 gTGGGCGCCTGGAGTTGCTTGGCGAGCAGCCTTGGGCACTGTCGGCAAGGGCACGCCAGCGACTGCGTGTGCGGGTTGGCCTGGTGCATCAGTCGCCGCCTTTGCCACCGCGTCAGCGGGTGGTGACTGCCGTACt                                                                >  1:940128/1‑136 (MQ=255)                                                                    ggCGCCTGGAGTTGCTTGGCGAGCAGCCTTGGGCACTGTCGGCAAGGGCACGCCAGCGACTGCGTGTGCGGGTTGGCCTGGTGCATCAGTCGCCGCCTTTGCCACCGCGTCAGCGGGTGGTGACTGCCGTACTGGc                                                             <  1:1006810/136‑1 (MQ=255)                                                                    ggCGCCTGGAGTTGCTTGGCGAGCAGCCTTGGGCACTGTCGGCAAGGGCACGCCAGCGACTGCGTGTGCGGGTTGGCCTGGTGCATCAGTCGCCGCCTTTGCCACCGCGTCAGCGGGTGGTGACTGCCGTACTGGc                                                             <  2:300556/136‑1 (MQ=255)                                                                    ggCGCCTGGAGTTGCTTGGCGAGCAGCCTTGGGCACTGTCGGCAAGGGCACGCCAGCGACTGCGTGTGCGGGTTGGCCTGGTGCATCAGTCGCCGCCTGTGCCACCGCGTCAGCGGGTGGTGACTGCCGTACTGGc                                                             <  1:1140774/136‑1 (MQ=255)                                                                       gcCTGGAGTTGCTTGGCGAGCAGCCTTGGGCACTGTCGGCAAGGGCACGCCAGCGACTGCGTGTGCGGGTTGGCCTGGTGCATCAGTCGCCGCCTTTGCCACCGCGTCAGCGGGTGGTGACTGCCGTACTGGCggg                                                          >  2:326568/1‑136 (MQ=255)                                                                       gcCTGGAGTTGCTTGGCGAGCAGCCTTGGGCACTGTCGGCAAGGGCACGCCAGCGACTGCGTGTGCGGGTTGGCCTGGTGCATCAGTCGCCGCCTTTGCCACCGCGTCAGCGGGTGGTGACTGCCGTACTGGCggg                                                          >  2:1224598/1‑136 (MQ=255)                                                                       gcCTGGAGTTGCTTGGCGAGCAGCCTTGGGCACTGTCGGCAAGGGCACGCCAGCGACTGCGTGTGCGGGTTGGCCTGGTGCATCAGTCGCCGCCTTTGCCACCGCGTCAGCGGGTGGTGACTGCCGTACTGGCggg                                                          >  1:1347538/1‑136 (MQ=255)                                                                         cTGGAGTTGCTTGGCGAGCAGCCTTGGGCACTGTCGGCAAGGGCACGCCAGCGACTGCGTGTGCGGGTTGGCCTGGTGCATCAGTCGCCGCCTTTGCCACCGCGTCAGCGGGTGGTGACTGCCGTACTGGCGGGTc                                                        <  2:10630/136‑1 (MQ=255)                                                                            gAGTTGCTTGGCGAGCAGCCTTGGGCACTGTCGGCAAGGGCACGCCAGCGACTGCGTGTGCGGGTTGGCCTGGTGCATCAGTCGCCGCCTTTGCCACCGCGTCAGCGGGTGGTGACTGCCGTACTGGCGGGTCGcc                                                     >  2:733243/1‑136 (MQ=255)                                                                              gTTGCTTGGCGAGCAGCCTTGGGCACTGTCGGCAAGGGCACGCCAGCGACTGCGTGTGCGGGTTGGCCTGGTGCATCAGTCGCCGCCTTTGCCACCGCGTCAGCGGGTGGTGACTGCCGTACTGGCGGGTCGCCTg                                                   <  1:1202853/136‑1 (MQ=255)                                                                              gTTGCTTGGCGAGCAGCCTTGGGCACTGTCGGCAAGGGCACGCCAGCGACTGCGTGTGCGGGTTGGCCTGGTGCATCAGTCGCCGCCTTTGCCACCGCGTCAGCGGGTGGTGACTGCCGTACTGGCGGGTCGCCTg                                                   <  2:968733/136‑1 (MQ=255)                                                                                tGCTTGGCGAGCAGCCTTGGGCACTGTCGGCAAGGGCTCGCCAGCGACTGCGTGTGCGGGTTGGCCTGGTGCATCAGTCGCCGCCTTTGCCACCGCGTCAGCGGGTGGTGACTGCCGTACTGGCGGGTCGCCTggg                                                 <  2:1331056/136‑1 (MQ=255)                                                                                         agcagcCTTGGGCACTGTCGGCAAGGGCACGCCAGCGACTGCGTGTGCGGGTTGGCCTGGTGCATCAGTCGCCGCCTTTGCCACCGCGTCAGCGGGTGGTGACTGCCGTACTGGCGGGTCGCCTGGGGCAGTgggg                                        >  2:760931/1‑136 (MQ=255)                                                                                          gcagcCTTGGGCACTGTCGGCAAGGGCACGCCAGCGACTGCGTGTGCGGGTTGGCCTGGTGCATCAGTCGCCGCCTTTGCCACCGCGTCAGCGGGTGGTGACTGCCGTACTGGCGGGTCGCCTGGGGCAGTggggg                                       >  1:117253/1‑136 (MQ=255)                                                                                          gcagcCTTGGGCACTGTCGGCAAGGGCACGCCAGCGACTGCGTGTGCGGGTTGGCCTGGTGCATCAGTCGCCGCCTTTGCCACCGCGTCAGCGGGTGGTGACTGCCGTACTGGCGGGTCGCCTGGGGCAGTggggg                                       >  1:466425/1‑136 (MQ=255)                                                                                          gcagcCTTGGGCACTGTCGGCAAGGGCACGCCAGCGACTGCGTGTGCGGGTTGGCCTGGTGCATCAGTCGCCGCCTTTGCCACCGCGTCAGCGGGTGGTGACTGCCGTACTGGCGGGTCGCCTGGGGCAGTggggg                                       >  1:460144/1‑136 (MQ=255)                                                                                              ccTTGGGCACTGTCGGCAAGGGCACGCCAGCGACTGCGTGTGCGGGTTGGCCTGGTGCATCAGTCGCCGCCTTTGCCACCGCGTCAGCGGGTGGTGACTGCCGTACTGGCGGGTCGCCTGGGGCAGTGGGGGACGc                                   <  2:714653/136‑1 (MQ=255)                                                                                                           cGGCAAGGGCACGCCAGCGACTGCGTGTGCGGGTTGGCCTGGTGCATCAGTCGCCGCCTTTGCCACCGCGTCAGCGGGTGGTGACTGCCGTACTGGCGGGTCGCCTGGGGCAGTGGGGGACGCTGCGCGGCTTGCt                      >  1:525688/1‑136 (MQ=255)                                                                                                           cGGCAAGGGCACGCCAGCGACTGCGTGTGCGGGTTGGCCTGGTGCATCAGTCGCCGCCTTTGCCACCGCGTCAGCGGGTGGTGACTGCCGTACTGGCGGGTCGCCTGGGGCAGTGGGGGACGCTGCGCGGCTTGCt                      >  1:523774/1‑136 (MQ=255)                                                                                                           cGGCAAGGGCACGCCAGCGACTGCGTGTGCGGGTTGGCCTGGTGCATCAGTCGCCGCCTTTGCCACCGCGTCAGCGGGTGGTGACTGCCGTACTGGCGGGTCGCCTGGGGCAGTGGGGGACGCTGCGCGGCTTGCt                      >  1:307409/1‑136 (MQ=255)                                                                                                           cGGCAAGGGCACGCCAGCGACTGCGTGTGCGGGTTGGCCTGGTGCATAAGTCGCCGCCTTTGCCACCGCGTCAGCGGGTGGTGACTGCCGTACTGGCGGGTCGCCAGGGGCAGTGGGGGACGCTGCGCGGCTTGcc                      >  1:277763/1‑135 (MQ=255)                                                                                                            ggCAAGGGCACGCCAGCGACTGCGTGTGCGGGTTGGCCTGGTGCATCAGTCGCCGCCTTTGCCACCGCGTCAGCGGGTGGTGACTGCCGTACTGGCGGGTCGCCTGGGGCAGTGGGGGACGCTGCGCGGCTTGCTc                     <  1:1085085/136‑1 (MQ=255)                                                                                                            ggCAAGGGCACGCCAGCGACTGCGTGTGCGGGTTGGCCTGGTGCATCAGTCGCCGCCTTTGCCACCGCGTCAGCGGGTGGTGACTGCCGTACTGGCGGGTCGCCTGGGGCAGTGGGGGACGCTGCGCGGCTTGCTc                     <  1:1195376/136‑1 (MQ=255)                                                                                                             gCAAGGGCACGCCAGCGACTGCGTGTGCGGGTTGGCCTGGTGCATCAGTCGCCGCCTTTGCCACCGCGTCAGCGGGTGGTGACTGCCGTACTGGc                                                             <  2:214993/95‑1 (MQ=255)                                                                                                             gCAAGGGCACGCCAGCGACTGCGTGTGCGGGTTGGCCTGGTGCATCAGTCGCCGCCTTTGCCACCGCGTCAGCGGGTGGTGACTGCCGTACTGGc                                                             >  1:214993/1‑95 (MQ=255)                                                                                                                aGGGCACGCCAGCGACTGCGTGTGCGGGTTGGCCTGGTGCATCAGTCGCCGCCTTTGCCACCGCGTCAGCGGGTGGTGACTGCCGTACTGGCGGGTCGCCTGGGGCAGTGGGGGACGCTGCGCGGCTTGCTCAAcc                 >  1:565969/1‑136 (MQ=255)                                                                                                                aGGGCACGCCAGCGACTGCGTGTGCGGGTTGGCCTGGTGCATCAGTCGCCGCCTTTGCCACCGCGTCAGCGGGTGGTGACTGCCGTACTGGCGGGTCGCCTGGGGCAGTGGGGGACGCTGCGCGGCTTGCTCAAcc                 >  1:498965/1‑136 (MQ=255)                                                                                                                 gggCACGCCAGCGACTGCGTGTGCGGGTTGGCCTGGTGCATCAGTCGCCGCCTTTGCCACCGCGTCAGCGGGTGGTGACTGCCGTACTGGCGGGTCGCCTGGGGCAGTGGGGGACGCTGCGCGGCTTGCTCAACct                <  1:326568/136‑1 (MQ=255)                                                                                                                       gCCAGCGACTGCGTGTGCGGGTTGGCCTGGTGCATCAGTCGCCGCCTTTGCCACCGCGTCAGCGGGTGGTGACTGCCGTACTGGCGGGTCGCCTGGGGCAGTGGGGGACGCTGCGCGGCTTGCTCAACctg               >  1:666738/1‑131 (MQ=255)                                                                                                                       gCCAGCGACTGCGTGTGCGGGTTGGCCTGGTGCATCAGTCGCCGCCTTTGCCACCGCGTCAGCGGGTGGTGACTGCCGTACTGGCGGGTCGCCTGGGGCAGTGGGGGACGCTGCGCGGCTTGCTCAACctg               <  2:666738/131‑1 (MQ=255)                                                                                                                       gCCAGCGACTGCGTGTGCGGGTTGGCCTGGTGCATCAGTCGCCGCCTTTGCCACCGCGTCAGCGGGTGGTGACTGCCGTACTGGCGGGTCGCCTGGGGCAGTGGGGGACGCTGCGCGGCTTGCTCAACCTGCTGTa          >  1:687477/1‑136 (MQ=255)                                                                                                                         cAGCGACTGCGTGTGCGGGTTGGCCTGGTGCATCAGTc                                                                                                          <  1:60665/38‑1 (MQ=255)                                                                                                                         cAGCGACTGCGTGTGCGGGTTGGCCTGGTGCATCAGTc                                                                                                          >  2:60665/1‑38 (MQ=255)                                                                                                                         cAGCGACTGCGTGTGCGGGTTGGCCTGGTGCATCAGTCGCCGCCTTTGCCACCGCGTCAGCGGGTGGTGACTGCCGTACTGGCGGGTCGCCTGGGGCAGTGGGGGACGCTGCGCGGCTTGCTCAACCTGCTGTATc        >  2:750150/1‑136 (MQ=255)                                                                                                                            cGACTGCGTGTGCGGGTTGGCCTGGTGCATCAGTCGCCGCCTTTGCCACCGCGTCAGCGGGTGGTGACTGCCGTACTGGCGGGTCGCCTGGGGCAGTGGGGGACGCTGCGCGGCTTGCTCAACCTGCTGTATCCCa     <  1:1119559/136‑1 (MQ=255)                                                                                                                             gACTGCGTGTGCGGGTTGGCCTGGTGCATCAGTCGCCGCCTTTGCCACCGCGTCAGCGGGTGGTGACTGCCGTACTGGCGGGTCGCCTGGGGCAGTGGGGGACGCTGCGCGGCTTGCTCAACCTGCTGTATCCCAg    <  1:579723/136‑1 (MQ=255)                                                                                                                               cTGCGTGTGCGGGTTGGCCTGGTGCATCAGTCGCCGCCTTTGCCACCGCGTCAGCGGGTGGTGACTGCCGTACTGGCGGGTCGCCTGGGGCAGTGGGGGACGCTGCGCGGCTTGCTCAACCTGCTGTATCCCAGCg  >  2:972204/1‑136 (MQ=255)                                                                                                                               cTGCGTGTGCGGGTTGGCCTGGTGCATCAGTCGCCGCCTTTGCCACCGCGTCAGCGGGTGGTGACTGCCGTACTGGCGGGTCGCCTGGGGCAGTGGGGGACGCTGCGCGGCTTGCTCAACCTGCTGTATCCCAGCg  >  1:1170358/1‑136 (MQ=255)                                                                                                                               cTGCGTGTGCGGGTTGGCCTGGTGCATCAGTCGCCGCCTTTGCCACCGCGTCAGCGGGTGGTGACTGCCGTACTGGCGGGTCGCCTGGGGCAGTGGGGGACGCTGCGCGGCTTGCTCAACCTGCTGTATCCCAGCg  >  2:1195104/1‑136 (MQ=255)                                                                                                                               cTGCGTGTGCGGGTTGGCCTGGTGCATCAGTCGCCGCCTTTGCCACCGCGTCAGCGGGTGGTGACTGCCGTACTGGCGGGTCGCCTGGGGCAGTGGGGGACGCTGCGCGGCTTGCTCAACCTGCTGTATCCCAGCg  >  2:1291976/1‑136 (MQ=255)                                                                                                                                      |                                                                                                                                 GGGCCGTCGGGGGCGGGCAAGTCCAGCTTGCTGCACCTGATGGCCACGGCCATCCAGCCCAGCAGTGGGCGCCTGGAGTTGCTTGGCGAGCAGCCTTGGGCACTGTCGGCAAGGGCACGCCAGCGACTGCGTGCGCGGGTTGGCCTGGTGCATCAGTCGCCGCCTTTGCCACCGCGTCAGCGGGTGGTGACTGCCGTACTGGCGGGTCGCCTGGGGCAGTGGGGGACGCTGCGCGGCTTGCTCAACCTGCTGTATCCCAGCG  >  NC_002947/963512‑963773

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑