Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_002947 5,489,180 G→A G180D (GGC→GAC)  PP_4824 → two‑component system sensor histidine kinase/response regulator

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029475,489,1800GA98.2% 189.8 / NA 55G180D (GGC→GAC) PP_4824two‑component system sensor histidine kinase/response regulator
Reads supporting (aligned to +/- strand):  ref base G (0/0);  major base A (18/36);  minor base C (1/0);  total (19/36)
Fisher's exact test for biased strand distribution p-value = 3.45e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GCAGGCAACCGCTGGACATCTACCTGCGCCTGGTCTCAGAGCACCAGCTGCGTCCGGCCATCAGCCTTGAGCCTGCGGCCGAGGCAGCCTCGGATCAACGCCAGCCGCTGCTGTTCGGCATGCTGTTCGGCGGCCTGATCATGCTGATCATGCACAACCTGATCCGCTTCCTCTACACCCGCTCCAGCACCACCCTGATCCTGGCGCTGTACCACGGCCTGATGCTGCTCAGCGGCCTGATCCTGCTGAACCTCAGC  >  NC_002947/5489048‑5489304
                                                                                                                                    |                                                                                                                            
gcagGCAACCGCTGGACATCTACCTGCGCCTGGTCTCAGAGCACCAGCTGCGTCCGGCCATCAGCCTTGAGCCTGCGGCCGAGGCAGCCTCGGATCAACGCCAGCCGCTGCTGTTCGGCATGCTGTTCGGCGACct                                                                                                                           >  2:811137/1‑136 (MQ=255)
 cagGCAACCGCTGGACATCTACCTGCGCCTGGTCTCAGAGCACCAGCTGCGTCCGGCCATCAGCCTTGAGCCTGCGGCCGAGGCAGCCTCGGATCAACGCCAGCCGCTGCTGTTCGGCATGCTGTTCGGCGACctg                                                                                                                          >  1:791519/1‑136 (MQ=255)
     cAACCGCTGGACATCTACCTGCGCCTGGTCTCAGAGCACCAGCTGCGTCCGGCCATCAGCCTTGAGCCTGCGGCCGAGGCAGCCTCGGATCAACGCCAGCCGCTGCTGTTCGGCATGCTGTTCGGCGACctgatca                                                                                                                      <  1:1154015/136‑1 (MQ=255)
     cAACCGCTGGACATCTACCTGCGCCTGGTCTCAGAGCACCAGCTGCGTCCGGCCATCAGCCTTGAGCCTGCGGCCGAGGCAGCCTCGGATCAACGCCAGCCGCTGCTGTTCGGCATGCTGTTCGGCGACctgatca                                                                                                                      <  1:146672/136‑1 (MQ=255)
     cAACCGCTGGACATCTACCTGCGCCTGGTCTCAGAGCACCAGCTGCGTCCGGCCATCAGCCTTGAGCCTGCGGCCGAGGCAGCCTCGGATCAACGCCAGCCGCTGCTGTTCGGCATGCTGTTCGGCGACctgatca                                                                                                                      <  1:525802/136‑1 (MQ=255)
         cGCTGGACATCTACCTGCGCCTGGTCTCAGAGCACCAGCTGCGTCCGGCCATCAGCCTTGAGCCTGCGGCCGAGGCAGCCTCGGATCAACGCCAGCCGCTGCTGTTCGGCATGCTGTTCGGCGACctgatcatgct                                                                                                                  <  2:138145/136‑1 (MQ=255)
         cGCTGGACATCTACCTGCGCCTGGTCTCAGAGCACCAGCTGCGTCCGGCCATCAGCCTTGAGCCTGCGGCCGAGGCAGCCTCGGATCAACGCCAGCCGCTGCTGTTCGGCATGCTGTTCGGCGACctgatcatgct                                                                                                                  <  2:297340/136‑1 (MQ=255)
         cGCTGGACATCTACCTGCGCCTGGTCTCAGAGCACCAGCTGCGTCCGGCCATCAGCCTTGAGCCTGCGGCCGAGGCAGCCTCGGATCAACGCCAGCCGCTGCTGTTCGGCATGCTGTTCGGCGACctgatcatgct                                                                                                                  <  1:1291165/136‑1 (MQ=255)
         cGCTGGACATCTACCTGCGCCTGGTCTCAGAGCACCAGCTGCGTCCGGCCATCAGCCTTGAGCCTGCGGCCGAGGCAGCCTCGGATCAACGCCAGCCGCTGCTGTTCGGCATGCTGTTCGGCGACctgatcatgct                                                                                                                  <  1:304339/136‑1 (MQ=255)
              gACATCTACCTGCGCCTGGTCTCAGAGCACCAGCTGCGTCCGGCCATCAGCCTTGAGCCTGCGGCCGAGGCAGCCTCGGATCAACGCCAGCCGCTGCTGTTCGGCATGCTGTTCGGCGAcct‑atcatgctgatcat                                                                                                            <  1:1355432/136‑1 (MQ=255)
              gACATCTACCTGCGCCTGGTCTCAGAGCACCAGCTGCGTCCGGCCATCAGCCTTGAGCCTGCGGCCGAGGCAGCCTCGGATCAACGCCAGCCGCTGCTGTTCGGCATGCTGTTCGGCGACctgatcatgctgatca                                                                                                             <  1:57891/136‑1 (MQ=255)
                      ccTGCGCCTGGTCTCAGAGCACCAGCTGCGTCCGGCCATCAGCCTTGAGCCTGCGGCCGAGGCAGCCTCGGATCAACGCCAGCCGCTGCTGTTCGGCATGCTGTTCGGCGACCTGATCATGCTGATCATGCACAAc                                                                                                     >  1:985257/1‑136 (MQ=255)
                            ccTGGTCTCAGAGCACCAGCTGCGTCCGGCCATCAGCCTTGAGCCTGCGGCCGAGGCAGCCTCGGATCAACGCCAGCCGCTGCTGTTCGGCATGCTGTTCGGCGACCTGATCATGCTGATCATGCACAACCTGATc                                                                                               <  2:302389/136‑1 (MQ=255)
                             cTGGTCTCAGAGCACCAGCTGCGTCCGGCCATCAGCCTTGAGCCTGCGGCCGAGGCAGCCTCGGATCAACGCCAGCCGCTGCTGTTCGGCATGCTGTTCGGCGACCTGATCATGCTGATCATGCACAACCTGATcc                                                                                              <  1:1071386/136‑1 (MQ=255)
                             cTGGTCTCAGAGCACCAGCTGCGTCCGGCCATCAGCCTTGAGCCTGCGGCCGAGGCAGCCTCGGATCAACGCCAGCCGCTGCTGTTCGGCATGCTGTTCGGCGACCTGATCATGCTGATCATGCACAACCTGATcc                                                                                              >  2:1345340/1‑136 (MQ=255)
                             cTGGTCTCAGAGCACCAGCTGCGTCCGGCCATCAGCCTTGAGCCTGCGGCCGAGGCAGCCTCGGATCAACGCCAGCCGCTGCTGTTCGGCATGCTGTTCGGCGACCTGATCATGCTGATCATGCACAACCTGATcc                                                                                              <  1:475431/136‑1 (MQ=255)
                             cTGGTCTCAGAGCACCAGCTGCGTCCGGCCATCAGCCTTGAGCCTGCGGCCGAGGCAGCCTCGGATCAACGCCAGCCGCTGCTGTTCGGCATGCTGTTCGGCGACCTGATCATGCTGATCATGCACAACCTGATcc                                                                                              >  1:395209/1‑136 (MQ=255)
                                  ctcAGAGCACCAGCTGCGTCCGGCCATCAGCCTTGAGCCTGCGGCCGAGGCAGCCTCGGATCAACGCCAGCCGCTGCTGTTCTGCATGCTGTTCGGCGACCTGATCATGCTGATCATGCACAACCTGATCCGCtta                                                                                         >  2:616935/1‑135 (MQ=255)
                                      gagCACCAGCTGCGTCCGGCCATCAGCCTTGAGCCTGCGGCCGAGGCAGCCTCGGATCAACGCCAGCCGCTGCTGTTCGGCATGCTGTTCGGCGACCTGATCATGCTGATCATGCACAACCTGATCCGCTTCctct                                                                                     <  1:906114/136‑1 (MQ=255)
                                        gCACCAGCTGCGTCCGGCCATCAGCCTTGAGCCTGCGGCCGAGGCAGCCTCGGATCAACGCCAGCCGCTGCTGTTCGGCATGCTGTTCGGCGACCTGATCATGCTGATCATGCACAACCTGATCCGCTTCCTCTac                                                                                   >  2:754545/1‑136 (MQ=255)
                                        gCACCAGCTGCGTCCGGCCATCAGCCTTGAGCCTGCGGCCGAGGCAGCCTCGGATCAACGCCAGCCGCTGCTGTTCGGCATGCTGTTCGGCGACCTGATCATGCTGATCATGCACAACCTGATCCGCTTCCTCTac                                                                                   <  2:625324/136‑1 (MQ=255)
                                        gCACCAGCTGCGTCCGGCCATCAGCCTTGAGCCTGCGGCCGAGGCAGCCTCGGATCAACGCCAGCCGCTGCTGTTCGGCATGCTGTTCGGCGACCTGATCATGCTGATCATGCACAACCTGATCCGCTTCCTCTac                                                                                   <  1:783965/136‑1 (MQ=255)
                                              gCTGCGTCCGGCCATCAGCCTTGAGCCTGCGGCCGAGGCAGCCTCGGATCAACGCCAGCCGCTGCTGTTCGGCATGCTGTTCGGCGACCTGATCATGCTGATCATGCACACCCTGATCCGCTTCCTCTACACgcgc                                                                             >  2:1169029/1‑136 (MQ=255)
                                              gCTGCGTCCGGCCATCAGCCTTGAGCCTGCGGCCGAGGCAGCCTCGGATCAACGCCAGCCGCTGCTGTTCGGCATGCTGTTCGGCGACCTGATCATGCTGATCATGCACAACCTGATCCGCTTCCTCTACACCCGc                                                                             >  1:168499/1‑136 (MQ=255)
                                               cTGCGTCCGGCCATCAGCCTTGAGCCTGCGGCCGAGGCAGCCTCGGATCAACGCCAGCCGCTGCTGTTCGGCATGCTGTTCGGCGACCTGATCATGCTGATCATGCACAACCTGATCCGCTTCCTCTACACCCGCt                                                                            >  1:1213942/1‑136 (MQ=255)
                                                  cGTCCGGCCATCAGCCTTGAGCCTGCGGCCGAGGCAGCCTCGGATCAACGCCAGCCGCTGCTGTTCGGCATGCTGTTCGGCGACCTGATCATGCTGATCATGCACAACCTGATCCGCTTCCTCTACACCCGCTCCa                                                                         <  1:405615/136‑1 (MQ=255)
                                                  cGTCCGGCCATCAGCCTTGAGCCTGCGGCCGAGGCAGCCTCGGATCAACGCCAGCCGCTGCTGTTCGGCATGCTGTTCGGCGACCTGATCATGCTGATCATGCACAACCTGATCCGCTTCCTCTACACCCGCTCCa                                                                         <  1:811137/136‑1 (MQ=255)
                                                  cGTCCGGCCATCAGCCTTGAGCCTGCGGCCGAGGCAGCCTCGGATCAACGCCAGCCGCTGCTGTTCGGCATGCTGTTCGGCGACCTGATCATGCTGATCATGCACAACCTGATCCGCTTCCTCTACACCCGCTCCa                                                                         <  1:351302/136‑1 (MQ=255)
                                                         ccATCAGCCTTGAGCCTGCGGCCGAGGCAGCCTCGGATCAACGCCAGCCGCTGCTGTTCGGCATGCTGTTCGGCGACCTGATCATGCTGATCATGCACAACCTGATCCGCTTCCTCTACACCCGCTCCAGcaccac                                                                  <  2:882102/136‑1 (MQ=255)
                                                         ccATCAGCCTTGAGCCTGCGGCCGAGGCAGCCTCGGATCAACGCCAGCCGCTGCTGTTCGGCATGCTGTTCGGCGACCTGATCATGCTGATCATGCACAACCTGATCCGCTTCCTCTACACCCGCTCCAGcaccac                                                                  <  1:218817/136‑1 (MQ=255)
                                                              tgCCTTGAGCCTGCGGCCGAGGCAGCCTCGGATCAACGGCAGCGGCTGCTGTTCGGCATGCTGTTCGGCGACCTGATCATGCTGATCATGCTCAACCTGATCCGCTTCCTCTACACCCGCTCCAGCACCACGCTGa                                                             <  2:533733/135‑1 (MQ=255)
                                                                   tGAGCCTGCGGCCGAGGCAGCCTCGGATCAACGCCAGCCGCTGCTGTTCGGCATGCTGTTCGGCGACCTGATCATGCTGATCATGCACAACCTGATCCGCTTCCTCTACACCCGCTCCAGCACCACCCTGATCCTg                                                        <  1:1232862/136‑1 (MQ=255)
                                                                   tGAGCCTGCGGCCGAGGCAGCCTCGGATCAACGCCAGCCGCTGCTGTTCGGCATGCTGTTCGGCGACCTGATCATGCTGATCATGCACAACCTGATCCGCTTCCTCTACACCCGCTCCAGCACCACCCTGATCCTg                                                        <  2:27610/136‑1 (MQ=255)
                                                                     aGCCTGCGGCCGAGGCAGCCTCGGATCAACGCCAGCCGCTGCTGTTCGGCATGCTGTTCGGCGACCTGATCATGCTGATCATGCACAACCTGATCCGCTTCCTCTACACCCGCTCCAGCACCACCCTGATCCTGgc                                                      <  2:1035114/136‑1 (MQ=255)
                                                                        cTGCGGCCGAGGCAGCCTCGGATCAACGCCAGCCGCTGCTGTTCGGCATGCTGTTCGGCGACCTGATCATGCTGATCATGCACAACCTGATCCGCTTCCTCTACACCCGCTCCAGCACCCCCCTGATCCTGgcgcg                                                   >  2:659729/1‑135 (MQ=255)
                                                                           cGGCCGAGGCAGCCTCGGATCAACGCCAGCCGCTGCTGTTCGGCATGCTGTTCGGCGACCTGATCATGCTGATCATGCACAACCTGATCCGCTTCCTCTACACCCGCTCCAGCACCACCCTGATCCTGGCGCTGTa                                                <  2:718068/136‑1 (MQ=255)
                                                                           cGGCCGAGGCAGCCTCGGATCAACGCCAGCCGCTGCTGTTCGGCATGCTGTTCGGCGACCTGATCATGCTGATCATGCACAACCTGATCCGCTTCCTCTACACCCGCTCCAGCACCACCCTGATCCTGGCGCTGTa                                                <  1:1042246/136‑1 (MQ=255)
                                                                           cGGCCGAGGCAGCCTCGGATCAACGCCAGCCGCTGCTGTTCGGCATGCTGTTCGGCGACCTGATCATGCTGATCATGCACAACCTGATCCGCTTCCTCTACACCCGCTCCAGCACCACCCTGATCCTGGCGCTGTa                                                <  2:293519/136‑1 (MQ=255)
                                                                           cGGCCGAGGCAGCCTCGGATCAACGCCAGCCGCTGCTGTTCGGCATGCTGTTCGGCGACCTGATCATGCTGATCATGCACAACCTGATCCGCTTCCTCTACACCCGCTCCAGCACCACCCTGATCCTGGCGCTGTa                                                <  2:997382/136‑1 (MQ=255)
                                                                           cGGCCGAGGCAGCCTCGGATCAACGCCAGCCGCTGCTGTTCGGCATGCTGTTCGGCGACCTGATCATGCTGATCATGCACAACCTGATCCGCTTCCTCTACACCCGCTCCAGCACCACCCTGATCCTGGCGCTGTa                                                <  2:1213942/136‑1 (MQ=255)
                                                                           cGGCCGAGGCAGCCTCGGATCAACGCCAGCCGCTGCTGTTCGGCATGCTGTTCGGCGACCTGATCATGCTGATCATGCACAACCTGATCCGCTTCCTCTACACCCGCTCCAGCACCACCCTGATCCTGGCGCTGTa                                                <  1:718505/136‑1 (MQ=255)
                                                                           cGGCCGAGGCAGCCTCGGATCAACGCCAGCCGCTGCTGTTCGGCATGCTGTTCGGCGACCTGATCATGCTGATCATGCACAACCTGATCCGCTTACTCTACACCCGCTCCAGCACCACCCTGATCCTGGCGCTGTa                                                <  1:923595/136‑1 (MQ=255)
                                                                             gCCGAGGCAGCCTCGGATCAACGCCAGCCGCTGCTGTTCGGCATGCTGTTCGGCGCCCTGATCATGCTGATCATGCACAACCTGATCCGCTTCCTCTACACCCGCTCCAGCACCACCCTGATCCTGGCGCTGTAcc                                              >  2:1357425/1‑136 (MQ=255)
                                                                             gCCGAGGCAGCCTCGGATCAACGCCAGCCGCTGCTGTTCGGCATGCTGTTCGGCGACCTGATCATGCTGATCATGCACAACCTGATCCGCTTCCTCTACACCCGCTCCAGCACCACCCTGATCCTGGCGCTGTAcc                                              >  2:209890/1‑136 (MQ=255)
                                                                             gCCGAGGCAGCCTCGGATCAACGCCAGCCGCTGCTGTTCGGCATGCTGTTCGGCGACCTGATCATGCTGATCATGCACAACCTGATCCGCTTCCTCTACACCCGCTCCAGCACCACCCTGATCCTGGCGCTGTAcc                                              >  2:1306223/1‑136 (MQ=255)
                                                                             gCCGAGGCAGCCTCGGATCAACGCCAGCCGCTGCTGTTCGGCATGCTGTTCGGCGACCTGATCATGCTGATCATGCACAACCTGATCCGCTTCCTCTACACCCGCTCCAGCACCACCCTGATCCTGGCGCTGTAcc                                              >  2:37306/1‑136 (MQ=255)
                                                                             gCCGAGGCAGCCTCGGATCAACGCCAGCCGCTGCTGTTCGGCATGCTGTTCGGCGACCTGATCATGCTGATCATGCACAACCTGATCCGCTTCCTCTACACCCGCTCCAGCACCACCCTGATCCTGGCGCTGTAcc                                              >  2:1148946/1‑136 (MQ=255)
                                                                             gCCGAGGCAGCCTCGGATCAACGCCAGCCGCTGCTGTTCGGCATGCTGTTCGGCGACCTGATCATGCTGATCATGCACAACCTGATCCGCTTCCTCTACACCCGCTCCAGCACCACCCTGATCCTGGCGCTGTAcc                                              >  1:525967/1‑136 (MQ=255)
                                                                                             aTCAACGCCAGCCGCTGCTGTTCGGCATGCTGTTCGGCGACCTGATCATGCTGATCATGCACAACCTGATCCGCTTCCTCTACACCCGCTCCAGCACCACCCTGATCCTGGCGCTGTACCACGGCCTGATgctgct                              <  1:405091/136‑1 (MQ=255)
                                                                                                  cGCCAGCCGCTGCTGTTCGGCATGCTGTTCGGCGACCTGATCATGCTGATCATGCACAACCTGATCCGCTTCGTCTACACCCGCTCCAGCACCACCCTGATCCTGGCGCTGTACCACGGCCTGATGCTGCTCAGCg                         <  2:1237470/136‑1 (MQ=255)
                                                                                                    ccAGCCGCTGCTGTTCGGCATGCTGTTCAGCGACCTGATCATGCTGATCATGCACAACCTGATCCGCTTCCTCTACACCCGCTCCAGCACCACCCTGATCCTGGCGCTGTACCACGGCCTGATGCTGCTCAGCGGc                       >  1:180595/1‑136 (MQ=255)
                                                                                                     cAGCCGCTGCTGTTCGGCATGCTGTTCGGCGACCTGATCATGCTGATCATGCACAACCTGATCCGCTTCCTCTACACCCGCTCCAGCACCACCCTGATCCTGGCGCTGTACCACGGCCTGATGCTGCTCAGCGGcc                      <  1:1363249/136‑1 (MQ=255)
                                                                                                          gctgctGTTCGGCATGCTGTTCGGCGACCTGATCATGCTGATCATGCACAACCTGATCCGCTTCCTCTACACCCGCTCCAGCACCACCCTGATCCTGgc                                                      <  2:143434/99‑1 (MQ=255)
                                                                                                          gctgctGTTCGGCATGCTGTTCGGCGACCTGATCATGCTGATCATGCACAACCTGATCCGCTTCCTCTACACCCGCTCCAGCACCACCCTGATCCTGgc                                                      >  1:143434/1‑99 (MQ=255)
                                                                                                                         gCTGTTCGGCGACCTGATCATGCTGATCATGCACAACCTGATCCGCTTCCTCTACACCCGCTCCAGCACCACCCTGATCCTGGCGCTGTACCACGGCCTGATGCTGCTCAGCGGCCTGATCCTGCTGAACCTCAGc  <  2:49239/136‑1 (MQ=255)
                                                                                                                                    |                                                                                                                            
GCAGGCAACCGCTGGACATCTACCTGCGCCTGGTCTCAGAGCACCAGCTGCGTCCGGCCATCAGCCTTGAGCCTGCGGCCGAGGCAGCCTCGGATCAACGCCAGCCGCTGCTGTTCGGCATGCTGTTCGGCGGCCTGATCATGCTGATCATGCACAACCTGATCCGCTTCCTCTACACCCGCTCCAGCACCACCCTGATCCTGGCGCTGTACCACGGCCTGATGCTGCTCAGCGGCCTGATCCTGCTGAACCTCAGC  >  NC_002947/5489048‑5489304

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑