Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_002947 1,201,211 C→T S134S (AGC→AGT) 
P5S (CCG→TCG) 		 xcpU →
xcpV →		 type II secretion pathway protein XcpU
type II secretion pathway protein XcpV

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029471,201,2110CT98.8% 303.6 / NA 86S134S (AGC→AGT) 
P5S (CCG→TCG) 		xcpU
xcpV		type II secretion pathway protein XcpU
type II secretion pathway protein XcpV
Reads supporting (aligned to +/- strand):  ref base C (0/0);  major base T (45/40);  minor base A (1/0);  total (46/40)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

TGGCCCAAGGGCCTGCACCCGGACTGGCCGGCCAGCCCGCAACCGCGCGTACTGTTCACGCCGCATGGCTGGGCGCAGGCGGGTAGTGTGCGCTGGCGCTGGGCCGATGGCAGTCAGCAATGGGCCTGGAGCCGCACCGATGGCATGTGGTTAGTGCCATGAAGCAAGCACAGCGTGGTTTCACTCTGCTGGAGGTGACCGTAGCCCTGGCAATCGCTGCCGTGCTGGCGGTGATCACCAGCCAGGTACTGCACCAGCGGC  >  NC_002947/1201080‑1201340
                                                                                                                                   |                                                                                                                                 
tGGCCCAAGGGCCTGCACCCGGACTGGCCGGCCAGCCCGCAACCGCGCGTACTGTTCACGCCGCATGGCTGGGCGCAGGCGGGTAGTGTGCGCTGGCGCTGGGCCGATGGCAGTCAGCAATGGGCCTGGAGTCGCa                                                                                                                               <  1:1364529/136‑1 (MQ=255)
  gCCCAAGGGCCTGCACCCGGACTGGCCGGCCAGCCCGCAACCGCGCGTACTGTTCACGCCGCATGGCTGGGCGCAGGCGGGTAGTGTGCGCTGGCGCTGGGCCGATGGCAGTCAGCAATGGGCCTGGAGTCGCAcc                                                                                                                             <  2:1160589/136‑1 (MQ=255)
  gCCCAAGGGCCTGCACCCGGACTGGCCGGCCAGCCCGCAACCGCGCGTACTGTTCACGCCGCATGGCTGGGCGCAGGCGGGTAGTGTGCGCTGGCGCTGGGCCGATGGCAGTCAGCAATGGGCCTGGAGTCGCAcc                                                                                                                             <  2:933186/136‑1 (MQ=255)
   cccAAGGGCCTGCACCCGGACTGGCCGGCCAGCCCGCAACCGCGCGTACTGTTCACGCCGCATGGCTGGGCGCAGGCGGGTAGTGTGCGCTGGCGCTGGGCCGATGGCAGTCAGCAATGGGCCTGGAGTCGCACCg                                                                                                                            >  2:84656/1‑136 (MQ=255)
     cAAGGGCCTGCACCCGGACTGGCCGGCCAGCCCGCAACCGCGCGTACTGTTCACGCCGCATGGCTGGGCGCAGGCGGGTAGTGTGCGCTGGCGCTGGGCCGATGGCAGTCAGCAATGGGCCTGGAGTCGCACCGAt                                                                                                                          <  2:929838/136‑1 (MQ=255)
     cAAGGGCCTGCACCCGGACTGGCCGGCCAGCCCGCAACCGCGCGTACTGTTCACGCCGCATGGCTGGGCGCAGGCGGGTAGTGTGCGCTGGCGCTGGGCCGATGGCAGTCAGCAATGGGCCTGGAGTCGCACCGAt                                                                                                                          >  2:721229/1‑136 (MQ=255)
     cAAGGGCCTGCACCCCGACTGGCCGGCCAGCCCGCAACCGCGCGTACTGTTCACGCCGCCTGGCTGGGCGCAGGCGGGTAGTGTGCGCTGGCGCTGGGCCGATGGCAGTCAGCAAAGGGCCTGGAGTCGCACCGAt                                                                                                                          >  1:1338008/1‑136 (MQ=255)
       aGGGCCTGCACCCGGACTGGCCGGCCAGCCCGCAACCGCGCGTACTGTTCACGCCGCATGGCTGGGCGCAGGCGGGTAGTGTGCGCTGGCGCTGGGCCGATGGCAGTCAGCAATGGGCCTGGAGTCGCACCGATgg                                                                                                                        <  1:721229/136‑1 (MQ=255)
       aGGGCCTGCACCCGGACTGGCCGGCCAGCCCGCAACCGCGCGTACTGTTCACGCCGCATGGCTGGGCGCAGGCGGGTAGTGTGCGCTGGCGCTGGGCCGATGGCAGTCAGCAATGGGCCTGGAGTCGCACCGATgg                                                                                                                        <  1:1151238/136‑1 (MQ=255)
       aGGGCCTGCACCCGGACTGGCCGGCCAGCCCGCAACCGCGCGTACTGTTCACGCCGCATGGCTGGGCGCAGGCGGGTAGTGTGCGCTGGCGCTGGGCCGATGGCAGTCAGCAATGGGCCTGGAGTCGCACCGATgg                                                                                                                        <  1:771026/136‑1 (MQ=255)
       aGGGCCTGCACCCGGACTGGCCGGCCAGCCCGCAACCGCGCGTACTGTTCACGCCGCATGGCTGGGCGCAGGCGGGTAGTGTGCGCTGGCGCTGGGCCGATGGCAGTCAGCAATGGGCCTGGAGTCGCACCGATgg                                                                                                                        <  1:411079/136‑1 (MQ=255)
       aGGGCCTGCACCCGGACTGGCCGGCCAGCCCGCAACCGCGCGTACTGTTCACGCCGCATGGCTGGGCGCAGGCGGGTAGTGTGCGCTGGCGCTGGGCCGATGGCAGTCAGCAATGGGCCTGGAGTCGCACCGATgg                                                                                                                        <  1:450750/136‑1 (MQ=255)
        gggCCTGCACCCGGACTGGCCGGCCAGCCCGCAACCGCGCGTACTGTTCACGCCGCATGGCTGGGCGCAGGCGGGTAGTGTGCGCTGGCGCTGGGCCGATGGCAGTCAGCAATGGGCCTGGAGTCGCACCGATGGc                                                                                                                       <  1:174501/136‑1 (MQ=255)
            cTGCACCCGGACTGGCCGGCCAGCCCGCAACCGCGCGTACTGTTCACGCCGCATGGCTGGGCGCAGGCGGGTAGTGTGCGCTGGCGCTGGGCCGATGGCAGTCAGCAATGGGCCTGGAGTCGCACCGATGGCAtgt                                                                                                                   <  2:217587/136‑1 (MQ=255)
              gCACCCGGACTGGCCGGCCAGCCCGCAACCGCGCGTACTGTTCACGCCGCATGGCTGGGCGCAGGCGGGTAGTGTGCGCTGGCGCTGGGCCGATGGCAGTCAGCAATGGGCCTGGAGTCGCACCGATGGCATGTgg                                                                                                                 <  1:323007/136‑1 (MQ=255)
              gCACCCGGAATCGCCGGCCAGCCAGCAAACGCGCGTAGTGTTCACGCCGCTTGGCTGGGCGCAGGCGGGTAGTGTGCGCTGGCGCTGGGCCGATGGCAGTCAGCAATGGGCCTGGAGTCGCACCGATGGCATGTgg                                                                                                                 <  2:490784/136‑1 (MQ=255)
               cACCCGGACTGGCCGGCCAGCCCGCAACCGCGCGTACTGTTCACGCCGCATGGCTGGGCGCAGGCGGGTAGTGTGCGCTGGCGCTGGGCCGATGGCAGTCAGCAATGGGCCTGGAGTCGCACCGATGGCATGTGGt                                                                                                                <  1:537/136‑1 (MQ=255)
                aCCCGGACTGGCCGGCCAGCCCGCAACCGCGCGTACTGTTCACGCCGCATGGCTGGGCGCAGGCGGGTAGTGTGCGCTGGCGCTGGGCCGATGGCAGTCAGCAATGGGCCTGGAGTCGCACCGATGGCATGTGGtt                                                                                                               >  2:237998/1‑136 (MQ=255)
                aCCCGGACTGGCCGGCCAGCCCGCAACCGCGCGTACTGTTCACGCCGCATGGCTGGGCGCAGGCGGGTAGTGTGCGCTGGCGCTGGGCCGATGGCAGTCAGCAATGGGCCTGGAGTCGCACCGATGGCATGTGGtt                                                                                                               >  2:1107492/1‑136 (MQ=255)
                aCCCGGACTGGCCGGCCAGCCCGCAACCGCGCGTACTGTTCACGCCGCATGGCTGGGCGCAGGCGGGTAGTGTGCGCTGGCGCTGGGCCGATGGCAGTCAGCAATGGGCCTGGAGTCGCACCGATGGCATGTGGtt                                                                                                               >  1:940777/1‑136 (MQ=255)
                aCCCGGACTGGCCGGCCAGCCCGCAACCGCGCGTACTGTTCACGCCGCATGGCTGGGCGCAGGCGGGTAGTGTGCGCTGGCGCTGGGCCGATGGCAGTCAGCAATGGGCCTGGAGTCGCACCGATCGCATGTGGtt                                                                                                               >  1:829198/1‑136 (MQ=255)
                     gACTGGCCGGCCAGCCCGCAACCGCGCGTACTGTTCACGCCGCATGGCTGGGCGCAGGCGGGTAGTGTGCGCTGGCGCTGGGCCGATGGCAGTCAGCAATGGGCCTGGAGTCGCACCGATGGCATGTGGTTAGTGc                                                                                                          <  1:322080/136‑1 (MQ=255)
                     gACTGGCCGGCCAGCCCGCAACCGCGCGTACTGTTCACGCCGCATGGCTGGGCGCAGGCGGGTAGTGTGCGCTGGCGCTGGGCCGATGGCAGTCAGCAATGGGCCTGGAGTCGCACCGATGGCATGTGGTTAGTGc                                                                                                          <  1:346674/136‑1 (MQ=255)
                     gACTGGCCGGCCAGCCCGCAACCGCGCGTACTGTTCACGCCGCATGGCTGGGCGCAGGCGGGTAGTGTGCGCTGGCGCTGGGCCGATGGCAGTCAGCAATGGGCCTGGAGTCGCACCGATGGCATGTGGTTAGTGc                                                                                                          <  2:682359/136‑1 (MQ=255)
                     gACTGGCCGGCCAGCCCGCAACCGCGCGTACTGTTCACGCCGCATGGCTGGGCGCAGGCGGGTAGTGTGCGCTGGCGCTGGGCCGATGGCAGTCAGCAATGGGCCTGGAGTCGCACCGATGGCATGTGGTTAGTGc                                                                                                          <  1:488146/136‑1 (MQ=255)
                     gACTGGCCGGCCAGCCCGCAACCGCGCGTACTGTTCACGCCGCATGGCTGGGCGCAGGCGGGTAGTGTGCGCTGGCGCTGGGCCGATGGCAGTCAGCAATGGGCCTGGAGTCGCACCGATGGCATGTGGTTAGTGc                                                                                                          <  2:1325381/136‑1 (MQ=255)
                     gACTGGCCGGCCAGCCCGCAACCGCGCGTACTGTTCACGCCGCATGGCTGGGCGCAGGCGGGTAGTGTGCGCTGGCGCTGGGCCGATGGCAGTCAGCAATGGGCCTGGAGTCGCACCGATGGCATGTGGTTAGTGc                                                                                                          <  1:1130525/136‑1 (MQ=255)
                       cTGGCCGGCCAGCCCGCAACCGCGCGTACTGTTCACGCCGCATGGCTGGGCGCAGGCGGGTAGTGTGCGCTGGCGCTGGGCCGATGGCAGTCAGCAATGGGCCTGGAGTCGCACCGATGGCATGTGGTTAGTGCCa                                                                                                        >  1:19339/1‑136 (MQ=255)
                       cTGGCCGGCCAGCCCGCAACCGCGCGTACTGTTCACGCCGCATGGCTGGGCGCAGGCGGGTAGTGTGCGCTGGCGCTGGGCCGATGGCAGTCAGCAATGGGCCTGGAGTCGCACCGATGGCATGTGGTTAGTGCCa                                                                                                        >  1:463852/1‑136 (MQ=255)
                       cTGGCCGGCCAGCCCGCAACCGCGCGTACTGTTCACGCCGCATGGCTGGGCGCAGGCGGGTAGTGTGCGCTGGCGCTGGGCCGATGGCAGTCAGCAATGGGCCTGGAGTCGCACCGATGGCATGTGGTTAGTGCCa                                                                                                        >  2:92425/1‑136 (MQ=255)
                        tGGCCGGCCAGCCCGCAACCGCGCGTACTGTTCACGCCGCATGGCTGGGCGCAGGCGGGTAGTGTGCGCTGGCGCTGGGCCGATGGCAGTCAGCAATGGGCCTGGAGTCGCACCGATGGCATGTGGTTAGTGCCAt                                                                                                       >  1:1366124/1‑136 (MQ=255)
                          gccggccAGCCCGCAACCGCGCGTACTGTTCACGCCGCATGGCTGGGCGCAGGCGGGTAGTGTGCGCTGGCGCTGGGCCGATGGCAGTCAGCAATGGGCCTGGAGTCGCACCGATGGCATGTGGTTAGTGCCATGa                                                                                                     <  1:739736/136‑1 (MQ=255)
                             ggccAGCCCGCAACCGCGCGTACTGTTCACGCCGCATGGCTGGGCGCAGGCGGGTAGTGTGCGCTGGCGCTGGGCCGATGGCAGTCAGCAATGGGCCTGGAGTCGCACCGATGGCATGTGGTTAGTGCCATGaagc                                                                                                  >  1:469463/1‑136 (MQ=255)
                             ggccAGCCCGCAACCGCGCGTACTGTTCACGCCGCATGGCTGGGCGCAGGCGGGTAGTGTGCGCTGGCGCTGGGCCGATGGCAGTCAGCAATGGGCCTGGAGTCGCACCGATGGCATGTGGTTAGTGCCATGaagc                                                                                                  >  1:136605/1‑136 (MQ=255)
                             ggccAGCCCGCAACCGCGCGTACTGTTCACGCCGCATGGCTGGGCGCAGGCGGGTAGTGTGCGCTGGCGCTGGGCCGATGGCAGTCAGCAATGGGCCTGGAGTCGCACCGATGGCATGTGGTTAGTGCCATGaagc                                                                                                  >  1:1151152/1‑136 (MQ=255)
                              gccAGCCCGCAACCGCGCGTACTGTTCACGCCGCATGGCTGGGCGCAGGCGGGTAGTGTGCGCTGGCGCTGGGCCGATGGCAGTCAGCAATGGGCCTGGAGTCGCACCGATGGCATGTGGTTAGTGCCATGaagca                                                                                                 >  1:293210/1‑136 (MQ=255)
                               ccAGCCCGCAACCGCGCGTACTGTTCACGCCGCATGGCTGGGCGCAGGCGGGTAGTGTGCGCTGGCGCTGGGCCGATGGCAGTCAGCAATGGGCCTGGAGTCGCACCGATGGCATGTGGTTAGTGCCATGaagcaa                                                                                                <  1:1081655/136‑1 (MQ=255)
                               ccAGCCCGCAACCGCGCGTACTGTTCACGCCGCATGGCTGGGCGCAGGCGGGTAGTGTGCGCTGGCGCTGGGCCGATGGCAGTCAGCAATGGGCCTGGAGTCGCACCGATGGCATGTGGTTAGTGCCATGaagcaa                                                                                                <  2:1289861/136‑1 (MQ=255)
                                     cGCAACCGCGCGTACTGTTCACGCCGCATGGCTGGGCGCAGGCGGGTAGTGTGCGCTGGCGCTGGGCCGATGGCAGTCAGCAATGGGCCTGGAGTCGCACCGATGGCATGTGGTTAGTGCCATGAAGCAAGCACAg                                                                                          >  1:645303/1‑136 (MQ=255)
                                             cgcgTACTGTTCACGCCGCATGGCTGGGCGCAGTCGGGTAGTGTGCGCTGGCGCTGGGCCGATGGCAGTCAGCAATGGGCCTGGAGTCGCACCGATGGCATGTGGTTAGTGCCATGAAGCAAGCACAGCGTGGttt                                                                                  <  2:518796/136‑1 (MQ=255)
                                             cgcgTACTGTTCACGCCGCATGGCTGGGCGCAGGCGGGTAGTGTGCGCTGGCGCTGGGCCGATGGCAGTCAGCAATGGGCCTGGAGTCGCACCGATGGCATGTGGTTAGTGCCATGAAGCAAGCACAGCGTGGttt                                                                                  <  1:335573/136‑1 (MQ=255)
                                              gcgTAGTGTTCACGCCGCATGGCTGGGCGCAGGCGGGTAGTGTGCGCTGGCGCTGGGCCGATGGCAGTCAGCAATGGGCCTGGAGTCGCACCGATGGCATGTGGTTAGTGCAATGAAGCAAGCACAGCGTGGTTTc                                                                                 <  1:270979/136‑1 (MQ=255)
                                                gTACTGTTCACGCCGCATGGCTGGGCGCAGGCGGGTAGTGTGCGCTGGCGCTGGGCCGATGGCAGTCAGCAATGGGCCTGGAGTCGCACCGATGGGATGTGGTTAGTGCCATGAAGCAAGCACAGCGTGGTTTCAc                                                                               >  2:980653/1‑136 (MQ=255)
                                                gTACTGTTCACGCCGCATGGCTGGGCGCAGGCGGGTAGTGTGCGCTGGCGCTGGGCCGATGGCAGTCAGCAATGGGCCTGGAGTCGCACCGATGGCATGTGGTTAGTGCCATGAAGCAAGCACAGCGTGGTTTCAc                                                                               >  2:1164284/1‑136 (MQ=255)
                                                gTACTGTTCACGCCGCATGGCTGGGCGCAGGCGGGTAGTGTGCGCTGGCGCTGGGCCGATGGCAGTCAGCAATGGGCCTGGAGACGCACCGATGGCATGTGGTTAGTGCCATGAAGCAAGCACAGCGTGGTTTCaa                                                                               >  2:1330532/1‑135 (MQ=255)
                                                        cACGCCGCATGGCTGGGCGCAGGCGGGTAGTGTGCGCTGGCGCTGGGCCGATGGCAGTCAGCAATGGGCCTGGAGTCGCACCGATGGCATGTGGTTAGTGCCATGAAGCAAGCACAGCGTGGTTTCACTCTGCTgg                                                                       <  2:1156765/136‑1 (MQ=255)
                                                        cACGCCGCATGGCTGGGCGCAGGCGGGTAGTGTGCGCTGGCGCTGGGCCGATGGCAGTCAGCAATGGGCCTGGAGTCGCACCGATGGCATGTGGTTAGTGCCATGAAGCAAGCACAGCGTGGTTTCACTCTGCTgg                                                                       >  2:1275379/1‑136 (MQ=255)
                                                         aCGCCGCATGGCTGGGCGCAGGCGGGTAGTGTGCGCTGGCGCTGGGCCGATGGCAGTCAGCAATGGGCCTGGAGTCGCACCGATGGCATGTGGTTAGTGCCATGAAGCAAGCACAGCGTGGTTTCACTCTGCTGGa                                                                      >  2:1207332/1‑136 (MQ=255)
                                                            ccgcATGGCTGGGCGCAGGCGGGTAGTGTGCGCTGGCGCTGGGCCGATGGCAGTCAGCAATGGGCCTGGAGTCGCACCGATGGCATGTGGTTAGTGCCATGAAGCAAGCACAGCGTGGTTTCACTCTGCTGGAGGt                                                                   >  2:652963/1‑136 (MQ=255)
                                                              gcATGGCTGGGCGCAGGCGGGTAGTGTGCGCTGGCGCTGGGCCGATGGCAGTCAGCAATGGGCCTGGAGTCGCACCGATGGCATGTGGTTAGTGCCATGAAGCAAGCACAGCGTGGTTTCACTCTGCTGGAGGTgc                                                                 >  2:815861/1‑135 (MQ=255)
                                                              gcATGGCTGGGCGCAGGCGGGTAGTGTGCGCTGGCGCTGGGCCGATGGCAGTCAGCAATGGGCCTGGAGTCGCACCGATGGCATGTGGTTAGTGCCATGAAGCAAGCACAGCGTGGTTTCACTCTGCTGGAGGTGa                                                                 >  1:1159600/1‑136 (MQ=255)
                                                              gcATGGCTGGGCGCAGGCGGGTAGTGTGCGCTGGCGCTGGGCCGATGGCAGTCAGCAATGGGCCTGGAGTCGCACCGATGGCATGTGGTTAGTGCCATGAAGCAAGCACAGCGTGGTTTCACTCTGCTGGAGGTGa                                                                 >  1:263667/1‑136 (MQ=255)
                                                              gcATGGCTGGGCGCAGGCGGGTAGTGTGCGCTGGCGCTGGGCCGATGGCAGTCAGCAATGGGCCTGGAGTCGCACCGATGGCATGTGGTTAGTGCCATGAAGCAAGCACAGCGTGGTTTCACTCTGCTGGAGGTGa                                                                 >  1:659085/1‑136 (MQ=255)
                                                              gcATGGCTGGGCGCAGGCGGGTAGTGTGCGCTGGCGCTGGGCCGATGGCAGTCAGCAATGGGCCTGGAGTCGCACCGATGGCATGTGGTTAGTGCCATGAAGCAAGCACAGCGTGGTTTCACTCTGCTGGAGGTGa                                                                 >  1:685112/1‑136 (MQ=255)
                                                                   gCTGGGCGCAGGCGGGTAGTGTGCGCTGGCGCTGGGCCGATGGCAGTCAGCAATGGGCCTGGAGTCGCACCGATGGCATGTGGTTAGTGCCATGAAGCAAGCACAGCGTGGTTTCACTCTGCTGGAGGTGACCGTa                                                            >  1:577444/1‑136 (MQ=255)
                                                                   gCTGGGCGCAGGCGGGTAGTGTGCGCTGGCGCTGGGCCGATGGCAGTCAGCAATGGGCCTGGAGTCGCACCGATGGCATGTGGTTAGTGCCATGAAGCAAGCACAGCGTGGTTTCACTCTGCTGGAGGTGACCGTa                                                            >  1:511505/1‑136 (MQ=255)
                                                                   gCTGGGCGCAGGCGGGTAGTGTGCGCTGGCGCTGGGCCGATGGCAGTCAGCAATGGGCCTGGAGTCGCACCGATGGCATGTGGTTAGTGCCATGAAGCAAGCACAGCGTGGTTTCACTCTGCTGGAGGTGACCGTa                                                            >  1:81087/1‑136 (MQ=255)
                                                                        gcgcAGGCGGGTAGTGTGCGCTGGCGCTGGGCCGATGGCAGTCAGCAATGGGCCTGGAGTCGCACCGATGGCATGTGGTTAGTGCCATGAAGCAAGCACAGCGTGGTTTCACTCTGCTGGAGGTGACCGTAGCCCt                                                       <  1:92425/136‑1 (MQ=255)
                                                                          gcAGGCGGGTAGTGTGCGCTGGCGCTGGGCCGATGGCAGTCAGCAATGGGCCTGGAGTCGCACCGATGGCATGTGGTTAGTGCCATGAAGCAAGCACAGCGTGGTTTCACTCTGCTGGAGGTGACCGTAGCCCTgg                                                     >  2:59275/1‑136 (MQ=255)
                                                                           ctggCGGGTTGTGTGCGCGGGCGCGTGGCCGATGGCAGTCAGCAATGGGCCTGGAGTCGCACCGATGGCATGTGGTTAGTGCCATGAAGCAAGCACAGCGTGGTTTCACTCTGCTGGAGGTGACCGTAGCCCTGGc                                                    <  2:725336/134‑1 (MQ=255)
                                                                                      tgtgCGCTGGCGCTGGGCCGATGGCAGTCAGCAATGGGCCTGGAGTCGCACCGATGGCATGTGGTTAGTGCCATGAAGCAAGCACAGCGTGGTTTCACTCTGCTGGAGGTGa                                                                 >  2:962851/1‑112 (MQ=255)
                                                                                      tgtgCGCTGGCGCTGGGCCGATGGCAGTCAGCAATGGGCCTGGAGTCGCACCGATGGCATGTGGTTAGTGCCATGAAGCAAGCACAGCGTGGTTTCACTCTGCTGGAGGTGa                                                                 <  1:962851/112‑1 (MQ=255)
                                                                                      tgtgCGCTGGCGCTGGGCCGATGGCAGTCAGCAATGGGCCTGGAGTCGCACCGATGGCATGTGGTTAGTGCCATGAAGCAAGCACAGCGTGGTTTCACTCTGCTGGAGGTGACCGTAGCCCTGGCAATCGCTGCCg                                         >  2:745767/1‑136 (MQ=255)
                                                                                           gctggcgctggGCCGATGGCAGTCAGCAATGGGCCTGGAGTCGCACCGATGGCATGTGGTTAGTGCCATGAAGCAAGCACAGCGTGGTTTCACTCTGCTGGAGGTGACCGTAGCCCTGGCAATCGCTGCCGTGCTg                                    >  2:942054/1‑136 (MQ=255)
                                                                                            ctggcgctggGCCGATGGCAGTCAGCAAT‑GGCCTGGAGTCGCACCGATGGCATGTGGTTAGTGCCATGAAGCAAGCACAGCGTGGTTTCACTCTGCTGGAGGTGACCGTAGCCCTGGCAATCGCTGCCGTGCTGGc                                  >  1:924858/1‑136 (MQ=255)
                                                                                             tggcgctggGCCGATGGCAGTCAGCAATGGGCCTGGAGTCGCACCGATGGCATGTGGTTAGTGCCATGAAGCAAGCACAGCGTGGTTTCACTCTGCTGGAGGTGACCGTAGCCCTGGCAATCGCTGCCGTGCTGGc                                  >  1:1144844/1‑136 (MQ=255)
                                                                                               gcgctggGCCGATGGCAGTCAGCAATGGGCCTGGAGTCGCACCGATGGCATGTGGTTAGTGCCATGAAGCAAGCACAGCGTGGTTTCACTCTGCTGGAGGTGACCGTAGCCCTGGCAATCGCTGCCGTGCTGGCgg                                >  2:726483/1‑136 (MQ=255)
                                                                                               gcgctggGCCGATGGCAGTCAGCAATGGGCCTGGAGTCGCACCGATGGCATGTGGTTAGTGCCATGAAGCAAGCACAGCGTGGTTTCACTCTGCTGGAGGTGACCGTAGCCCTGGCAATCGCTGCCGTGCTGGCgg                                >  1:1010090/1‑136 (MQ=255)
                                                                                                cgctggGCCGATGGCAGTCAGCAATGGGCCTGGAGTCGCACCGATGGCATGTGGTTAGTGCCATGAAGCAAGCACAGCGTAGTTTCACTCTGCTGGAGGTGACCGTAGCCCTGGCAATCGCTGCCGTGCTGGCGGt                               >  2:289004/1‑136 (MQ=255)
                                                                                                   tggGCCGATGGCAGTCAGCAATGGGCCTGGAGTCGCACCGATGGCATGTGGTTAGTGCCATGAAGCAAGCACAGCGTTGTTTCACTCTGCTGGAGGTGACCGTAGCCCTGGCAATCGCTGCCGTGCTGGCGGTGAt                            <  1:289004/136‑1 (MQ=255)
                                                                                                   tggGCCGATGGCAGTCAGCAATGGGCCTGGAGTCGCACCGATGGCATGTGGTTAGTGCCATGAAGCAAGCACAGCGTGGTTTCACTCTGCTGGAGGTGACCGTAGCCCTGGCAATCGCTGCCGTGCTGGCGGTGAt                            <  1:894551/136‑1 (MQ=255)
                                                                                                   tggGCCGATGGCAGTCAGCAATGGGCCTGGAGTCGCACCGATGGCATGTGGTTAGTGCCATGAAGCAAGCACAGCGTGGTTTCACTCTGCTGGAGGTGACCGTAGCCCTGGCAATCGCTGCCGTGCTGGCGGTGAt                            <  2:291107/136‑1 (MQ=255)
                                                                                                        ggATGGCAGTCAGCAATGGGCCTGGAGTCGCACCGATGGCATGTGGTTAGTGCCATGAAGCAAGCACAGCGTGGTTTCACTCTGCTGGAGGTGACCGTAGCCCTGGCAATCGCTGCCGTGCTGGCGGTGATCAcca                       <  1:193461/135‑1 (MQ=255)
                                                                                                        cGATGGCAGTCAGCAATGGGCCTGGAGTCGCACCGATGGCATGTGGTTAGTGCCATGAAGCAAGCACAGCGTGGTTTCACTCTGCTGGAGGTGACCGTAGCCCTGGCAATCGCTGCCGTGCTGGCGGTGATCAcca                       <  2:469463/136‑1 (MQ=255)
                                                                                                        cGATGGCAGTCAGCAATGGGCCTGGAGTCGCACCGATGGCATGTGGTTAGTGCCATGAAGCAAGCACAGCGTGGTTTCACTCTGCTGGAGGTGACCGTAGCCCTGGCAATCGCTGCCGTGCTGGCGGTGATCAcca                       <  2:1157278/136‑1 (MQ=255)
                                                                                                             gCAGTCAGCAATGGGCCTGGAGTCGCACCGATGGCATGTGGTTAGTGCCATGAAGCAAGCACAGCGTGGTTTCACTCTGCTGGAGGTGACCGTAGCCCTGGCAATCGCTGCCGTGCTGGCGGTGATCAccagccag                  >  2:799216/1‑136 (MQ=255)
                                                                                                              cAGTCAGCAATGGGCCTGGAGTCGCACCGATGGCATGTGGTTAGTGCCATGAAGCAAGCACAGCGTGGttt                                                                                  >  2:1322210/1‑71 (MQ=255)
                                                                                                              cAGTCAGCAATGGGCCTGGAGTCGCACCGATGGCATGTGGTTAGTGCCATGAAGCAAGCACAGCGTGGttt                                                                                  <  1:1322210/71‑1 (MQ=255)
                                                                                                               aGTCAGCAATGGGCCTGGAGTCGCACCGATGGCATGTGGTTAGTGCCATGAAGCAAGCACAGCGTGGTTTCACTCTGCTGGAGGTGACCGTAGCCCTGGCAATCGCTGCCGTGCTGGCGGTGATCACCAGCCAGGt                <  2:291259/136‑1 (MQ=255)
                                                                                                                    gCAATGGGCCTGGAGTCGCACCGATGGCATGTGGTTAGTGCCATGAAGCAAGCACAGCGTGGTTTCACTCTGCTGGAGGTGACCGTAGCCCTGGCAATCGCTGCCGTGCTGGCGGTGATCACCAGCCAGGTACTGc           >  1:562959/1‑136 (MQ=255)
                                                                                                                      aaTGGGCCTGGAGTCGCACCGATGGCATGTGGTTAGTGCCATGAAGCAAGCACAGCGTGGTTTCACTCTGCTGGAGGTGACCGTAGCCCTGGCAATCGCTGCCGTGCTGGCGGTGATCACCAGCCAGGTACTGCAc         >  1:349013/1‑136 (MQ=255)
                                                                                                                             cTGGAGTCGCACCGATGGCATGTGGTTAGTGCCATGAAGCAAGCACAGCGTGGTTTCACTCTGCTGGAGGTGACTGTAGCCCTGGCAATCGCTGCCGTGCTGGCGGTGATCACCAGCCAGGTACTGCACCAGCGGc  >  2:1308276/1‑136 (MQ=255)
                                                                                                                             cTGGAGTCGCACCGATGGCATGTGGTTAGTGCCATGAAGCAAGCACAGCGTGGTTTCACTCTGCTGGAGGTGACCGTAGCCCTGGCAATCGCTGCCGTGCTGGCGGTGATCACCAGCCAGGTACTGCACCAGCGGc  <  1:433408/136‑1 (MQ=255)
                                                                                                                             cTGGAGTCGCACCGATGGCATGTGGTTAGTGCCATGAAGCAAGCACAGCGTGGTTTCACTCTGCTGGAGGTGACCGTAGCCCTGGCAATCGCTGCCGTGCTGGCGGTGATCACCAGCCAGGTACTGCACCAGCGGc  >  2:1288003/1‑136 (MQ=255)
                                                                                                                             cTGGAGTCGCACCGATGGCATGTGGTTAGTGCCATGAAGCAAGCACAGCGTGGTTTCACTCTGCTGGAGGTGACCGTAGCCCTGGCAATCGCTGCCGTGCTGGCGGTGATCACCAGCCAGGTACTGCACCAGCGGc  >  2:1282543/1‑136 (MQ=255)
                                                                                                                             cTGGAGTCGCACCGATGGCATGTGGTTAGTGCCATGAAGCAAGCACAGCGTGGTTTCACTCTGCTGGAGGTGACCGTAGCCCTGGCAATCGCTGCCGTGCTGGCGGTGATCACCAGCCAGGTACTGCACCAGCGGc  <  2:293210/136‑1 (MQ=255)
                                                                                                                                   |                                                                                                                                 
TGGCCCAAGGGCCTGCACCCGGACTGGCCGGCCAGCCCGCAACCGCGCGTACTGTTCACGCCGCATGGCTGGGCGCAGGCGGGTAGTGTGCGCTGGCGCTGGGCCGATGGCAGTCAGCAATGGGCCTGGAGCCGCACCGATGGCATGTGGTTAGTGCCATGAAGCAAGCACAGCGTGGTTTCACTCTGCTGGAGGTGACCGTAGCCCTGGCAATCGCTGCCGTGCTGGCGGTGATCACCAGCCAGGTACTGCACCAGCGGC  >  NC_002947/1201080‑1201340

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑