Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_002947	1,348,789	A→G	intergenic (‑249/+505)	galP‑I ← / ← PP_1174	porin‑like protein/hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	1,348,789	0	A	G	100.0%	174.0 / NA	48	intergenic (‑249/+505)	galP‑I/PP_1174	porin‑like protein/hypothetical protein
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (20/28);  total (20/28)

GCGCTACGTACTGGCAGGCGTACGCGAGCTGGCCGGGGGAAGAGGGGGGGCAACAGGTGCGGGTGCAGCGGGATGGGTGTCGACATGGGTTCGTCCACGGCTTGTTGTTTTTGTTATGTGTTGTCGTACAACTATGGGTGATTATTAGCAAGGGGAAGATGGGCTGTCAACGGCTGATAGCTGAGTAATAAAGGTCCAAGGACCCAAAATCCGGGCTGCAGAAGCTCTGCCGTGGACTCTGTACAGACA  >  NC_002947/1348659‑1348907                                                                                                                                   |                                                                                                                       gcgcTACGTACTGGCAGGCGTACGCGAGCTGGCCGGGGGAAGAGGGGGGGCAACAGGTGCGGGTGCAGCGGGATGGGTGTCGACATGGGTTCGTCCACGGCTTGTTGTTTTTGTTATGTGTTGTCGTACAGCTATg                                                                                                                   <  2:659270/136‑1 (MQ=255)   gcTACGTACTGGCAGGCGTACGCGAGCTGGCCGGGGGAAGAGGGGGGGCAACAGGTGCGGGTGCAGCGGGATGGGTGTCGACATGGGTTCGTCCACGGCTTGTTGTTTTTGTTATGTGTTGTCGTACAGCTATggg                                                                                                                 >  2:1311914/1‑136 (MQ=255)     tACGTACTGGCAGGCGTACGCGAGCTGGCCGGGGGAAGAGGGGGGGCAACAGGTGCGGGTGCAGCGGGATGGGTGTCGACATGGGTTCGTCCGCGGCTTGTTGTTTTTGTTATGTGTTGTCGTACAGCTATGGGTg                                                                                                               >  1:969226/1‑136 (MQ=255)           cTGGCAGGCGTACGCGAGCTGGCCGGGGGAAGAGGGGGGGCAACAGGGGCGGGTGCAGCGGGATGGGTGTCGACATGGGTTCGTCCACGGCTTGTTGTTTTTGTTATGTGTTGTCGTACAGCTATGGGTGattatt                                                                                                         <  2:288302/136‑1 (MQ=255)                 ggCGTACGCGAGCTGGCCGGGGGAAGAGGGGGGGCAACAGGTGCGGGTGCAGCGGGATGGGTGTCGACATGGGTTCGTCCACGGCTTGTTGTTTTTGTTATGTGTTGTCGTACAGCTATGGGTGATTATTAGCAAg                                                                                                   >  2:754367/1‑136 (MQ=255)                    gTACGCGAGCTGGCCGGGGGAAGAGGGGGGGCAACAGGTGCGGGTGCAGCGGGATGGGTGTCGACATGGGTTCGTCCACGGCTTGTTGTTTTTGTTATGTGTTGTCGTACAGCTATGGGTGATTATTAGCAAgggg                                                                                                >  2:1000395/1‑136 (MQ=255)                      cggcgAGCTGGCCGGGGCATGAGGGGCGGCAACAGGTGCGGGTGCAGCGGGATGGGTGTCGACATGGGTTCGTCCACGGCTTGTTGTTTTTGTTATGTGTTGTCGTACAGCTATGGGTGATTATTAGCAAGGGGaa                                                                                              <  2:241736/134‑1 (MQ=255)                      aCGCGAGCTGGCCGGGGGAAGAGGGGGGGCAACAGGTGCGGGTGCAGCGGGATGGGTGTCGACATGGGTTCGTCCACGGCTTGTTGTTTTTGTTATGTGTTGTCGTACAGCTATGGGTGATTATTAGCAAGGGGaa                                                                                              >  1:829730/1‑136 (MQ=255)                           tgCTGGCCGGGGGAAGAGGGGGGGCAACAGGTGCGGGTGCAGCGGGATGGGTGTCGACATGGGTTCGTCCACGGCTTGTTGTTTTTGTTATGTGTTGTCGTACAGCTATGGGTGATTATTAGCAAGGGGAAGATgg                                                                                         <  2:676046/135‑1 (MQ=255)                                ggcGGGGGAAGAGGGGGGGCATCTGGTGCGGGTGCAGGGGGTTGGGTGTCGACATGGGTTGGTGCACGGCTTGTTGTTTTTGTTATGTGTTGTCGTACAGCTTTGGGTGATTATTAGCAAGGGGAAGATGGGCTGt                                                                                    <  2:933520/134‑1 (MQ=255)                                gCCGGGGGAAGAGGGGGGGCAACAGGTGCGGGTGCAGCGGGATGGGTGTCGACATGGGTTCGTCCACGGCTTGTTGTTTTTGTTATGTGTTGTCGTACAGCTATGGGTGATTATTAGCAAGGGGAAGATGGGCTGt                                                                                    <  1:246337/136‑1 (MQ=255)                                gCCGGGGGAAGAGGGGGGGCAACAGGTGCGGGTGCAGCGGGATGGGTGTCGACATGGGTTCGTCCACGGCTTGTTGTTTTTGTTATGTGTTGTCGTACAGCTATGGGTGATTATTAGCAAGGGGAAGATGGGCTGt                                                                                    <  1:96055/136‑1 (MQ=255)                                gCCGGGGGAAGAGGGGGGGCAACAGGTGCGGGTGCAGCGGGATGGGTGTCGACATGGGTTCGTCCACGGCTTGTTGTTTTTGTTATGTGTTGTCGTACAGCTATGGGTGATTATTAGCAAGGGGAAGATGGGCTGt                                                                                    >  1:1342043/1‑136 (MQ=255)                                          gagGGGGGGCAAGAGGTGGGGGGGGAGCGGGATGGGTGTCGACATGGGTTCGTCCTCGGCTTGTTGTTTTTGTTTTGTGTTGTCGTACAGCTATTGGTGATTTTTAGCAAGGGGAAGATGGGCTGTCAACGGCTGa                                                                          <  1:303793/136‑1 (MQ=255)                                             ggggggCAACAGGTGCGGGTGCAGCGGGATGGGTGTCGACATGGGTTCGTCCACGGCTTGTTGTTTTTGTTATGTGTTGTCGTACAGCTATGGGTGATTATTAGCAAGGGGAAGATGGGCTGTCAACGGCTGATAg                                                                       <  1:1134964/136‑1 (MQ=255)                                                    aaCAGGTGCGGGTGCAGCGGGATGGGTGTCGACATGGGTTCGTCCACGGCTTGTTGTTTTTGTTATGTGTTGTCGTACAGCTATGGGTGATTATTAGCAAGGGGAAGATGGGCTGTCAACGGCTGATAGCTGAGta                                                                >  2:29726/1‑136 (MQ=255)                                                    aaCAGGTGCGGGTGCAGCGGGATGGGTGTCGACATGGGTTCGTCCACGGCTTGTTGTTTTTGTTATGTGTTGTCGTACAGCTATGGGTGATTATTAGCAAGGGGAAGATGGGCTGTCAACGGCTGATAGCTGAGta                                                                >  2:333379/1‑136 (MQ=255)                                                        ggTGCGGGTGCAGCGGGATGGGTGTCGACATGGGTTCGTCCACGGCTTGTTGTTTTTGTGATGTGTTGTCGTACAGCTATGGGTGATTATTAGCTAGGGGAAGATGGGCTGTCAACGGCTGATAGCTGAGtaataa                                                            >  2:489859/1‑136 (MQ=255)                                                               gTGCAGCGGGATGGGTGTCGACATGGGTTCGTCCACGGCTTGTTGTTTTTGTTATGTGTTGTCGTACAGCTATGGGTGat                                                                                                             >  1:1373260/1‑80 (MQ=255)                                                               gTGCAGCGGGATGGGTGTCGACATGGGTTCGTCCACGGCTTGTTGTTTTTGTTATGTGTTGTCGTACAGCTATGGGTGat                                                                                                             <  2:1373260/80‑1 (MQ=255)                                                               gTGCAGCGGGATGGGTGTCGACATGGGTTCGTCCACGGCTTGTTGTTTTTGTTATGTGTTGTCGTACAGCTATGGGTGATTATTAGCAAGGGGAAGATGGGCTGTCAACGGCTGATAGCTGAGTAATAAAGGTCCa                                                     >  1:439105/1‑136 (MQ=255)                                                                      gggATGGGTGTCGACATGGGTTCGTCCACGGCTTGTTGTTTTTGTTATGTGTTGTCGTACAGCTATGGGTGATTATTAGCAAGGGGAAGATGGGCTGTCAACGGCTGATAGCTGAGTAATAAAGGTCCAAGGAccc                                              >  1:142524/1‑136 (MQ=255)                                                                              tgtCGACATGGGTTCGTCCACGGCTTGTTGTTTTTGTTATGTGTTGTCGTACAGCTATGGGTGATTATTAGCAAGGGGAAGATGGGCTGTCAACGGCTGATAGCTGAGTAATAAAGGTCCAAGGACCCAAAATCCg                                      <  1:216906/136‑1 (MQ=255)                                                                                   aCATGGGGTCGTCCACGGCTTGTTGTTTTTGTTATGTGTTGTCGTACAGCTATGGGTGATTATTAGCAAGGGGAAGATGGGCTGTCAACGGCTGATAGCTGAGTAATAAAGGTCCAAGGACCCAAAATCCGGGCTg                                 <  2:923824/136‑1 (MQ=255)                                                                                    cATGGGTTCGTCCACGGCTTGTTGTTTTTGTTATGTGTTGTCGTACAGCTATGGGTGATTATTAGCAAGGGGAAGATGGGCTGTCAACGGCTGATAGCTGAGTAATAAAGGTCCAAGGACCCAAAATCCGGGCTGc                                >  1:240185/1‑136 (MQ=255)                                                                                        ggTTCGTCCACGGCTTGTTGTTTTTGTTATGTGTTGTCGTACAGCTATGGGTGATTATTAGCAAGGGGGAGATGGGCTGTCAACGGCTGATAGCTGAGTAATAAAGGTCCAAGGACCCAAAATCCGGGCTGCAGaa                            <  1:464436/136‑1 (MQ=255)                                                                                        ggTTCGTCCACGGCTTGTTGTTTTTGTTATGTGTTGTCGTACAGCTATGGGTGATTATTAGCAAGGGGAAGATGGGCTGTCAACGGCTGATAGCTGAGTAATAAAGGTCCAAGGACCCAAAATCCGGGCTGCAGaa                            <  1:698773/136‑1 (MQ=255)                                                                                        ggTTCGTCCACGGCTTGTTGTTTTTGTTATGTGTTGTCGTACAGCTATGGGTGATTATTAGCAAGGGGAAGATGGGCTGTCAACGGCTGATAGCTGAGTAATAAAGGTCCAAGGACCCAAAATCCGGGCTGCAGaa                            <  1:1000395/136‑1 (MQ=255)                                                                                        ggTTCGTCCACGGCTTATTGTTTTTGTTATGTGTTGTCGTACAGCTATGGGTGATTATTAGCAAGGGGAAGATGGGCTGTCAACGGCTGATAGCTGAGTAATAAAGGTCCAAGGACCCAAAATCCGGGCTGCAGaa                            <  1:95022/136‑1 (MQ=255)                                                                                           tCGTCCACGGCTTGTTGTTTTTGTTATGTGTTGTCGTACAGCTATg                                                                                                                   <  1:861929/46‑1 (MQ=255)                                                                                           tCGTCCACGGCTTGTTGTTTTTGTTATGTGTTGTCGTACAGCTATg                                                                                                                   >  2:861929/1‑46 (MQ=255)                                                                                             gTCCACGGCTTGTTGTTTTTGTTATGTGTTGTCGTACAGCTATGGGTGATTATTAGCAAGGGGAAGATGGGCTGTCAACGGCTGATAGCTGAGTAATAAAGGTCCAAGGACCCAAAATCCGGGCTGCAGAAGctct                       >  1:918038/1‑136 (MQ=255)                                                                                               ccACGGCTTGTTGTTTTTGTTATGTGTTGTCGTACTGCTATGGGTGATTATTAGCAAGGGGAAGATGGGCTGTCAACGGCTGATAGCTGAGTAATAAAGGTCCAAGGACCCAAAATCCGGGCTGCAGAAGCTCTGc                     <  1:129036/136‑1 (MQ=255)                                                                                               ccACGGCTTGTTGTTTTTGTTATGTGTTGTCGTACAGCTATGGGTGATTATTAGCAAGGGGAAGATGGGCTGTCAACGGCTGATAGCTGAGTAATAAAGGTCCAAGGACCCAAAATCCGGGCTGCAGAAGCTCTGc                     <  2:142524/136‑1 (MQ=255)                                                                                               ccACGGCTTGTTGTTTTTGTTATGTGTTGTCGTACAGCTATGGGTGATTATTAGCAAGGGGAAGATGGGCTGTCAACGGCTGATAGCTGAGTAATAAAGGTCCAAGGACCCAAAATCCGGGCTGCAGAAGCTCTGc                     <  1:1311914/136‑1 (MQ=255)                                                                                                 aCGGCTTGTTGTTTTTGTTATGTGTTGTCGTACAGCTATGGGTGATTATTAGCAAGGGGAAGATGGGCTGTCAACGGCTGATAGCTGAGTAATAAAGGTCCAAGGACCCAAAATCCGGGCTGCAGAAGCTCTGCCg                   >  2:1167684/1‑136 (MQ=255)                                                                                                 aCGGCTTGTTGTTTTTGTTATGTGTTGTCGTACAGCTATGGGTGATTATTAGCAAGGGGAAGATGGGCTGTCAACGGCTGATAGCTGAGTAATAAAGGTCCAAGGACCCAAAATCCGGGCTGCAGAAGCTCTGCCg                   >  1:158252/1‑136 (MQ=255)                                                                                                  cGGCTTGTTGTTTTTGTTATGTGTTGTCGTACAGCTTTGGGTGATTATTAGCATGGGGAAGATGGGCTGTCAACGGCTGATAGCTGAGTAATAAAGGTCCAAGGACCCAAAATCCGGGCTGCAGAAGCTCTGCCGt                  <  2:1364786/136‑1 (MQ=255)                                                                                                  cGGCTTGTTGTTTTTGTTATGTGTTGTCGTACAGCTATGGGTGATTATTAGCAAGGTGAAGATGGGCTGTCAACGGCTGATAGCTGAGTAATAAAGGTCCAAGGACCCAAAATCCGGGCTGCAGAAGCTCTGCCGt                  <  2:538874/136‑1 (MQ=255)                                                                                                    gCTTGTTGTTTTTGTTATGTGTTGTCGTACAGCTATGGGTGATTATTAGCAAGGTGAAGATGGGCTGTCAACGGCTGATAGCTGAGTAATAAAGGTCCAAGGACCCAAAATCCGGGCTGCAGAAGCTCTGCCGTgg                <  2:14292/136‑1 (MQ=255)                                                                                                    gCTTGTTGTTTTTGTTATGTGTTGTCGTACAGCTATGGGTGATTATTAGCAAGGGGAAGATGGGCTGTCAACGGCTGATAGCTGAGTAATAAAGGTCCAAGGACCCAAAATCCGGGCTGCAGAAGCTCTGCCGTgg                <  1:75171/136‑1 (MQ=255)                                                                                                         ttgttTTTGTTATGTGTTGTCGTACAGCTATGGGTGATTATTAGCAAGGGGAAGATGGGCTGTCAACGGCTGATAGCTGAGTAATAAAGGTCCAAGGACCCAAAATCCGGGCTGCAGAAGCTCTGCCGTGGActct           <  1:497451/136‑1 (MQ=255)                                                                                                           gttTTTGTTATGTGTTGTCGTACAGCTATGGGTGATTATTAGCAAGGGGAAGATGGGCTGTCAACGGCTGATAGCTGAGTAATAAAGGTCCAAGGACCCAAAATCCGGGCTGCAGAAGCTCTGCCGTGGACTCTGt         >  1:1010154/1‑136 (MQ=255)                                                                                                            tttttGTTATGTGTTGTCGTACAGCTATGGGTGATTATTAGCAAGGGGAAGATGGGCTGTCAACGGCTGATAGCTGAGTAATAAAGGTCCAAGGACCCAAAATCCGGGCTGCAGAAGCTCTGCCGTGGACTCTGTa        >  1:830080/1‑136 (MQ=255)                                                                                                             ttttGTTATGTGTTGTCGTACAGCTATGGGTGATTATTAGCAAGGGGAAGATGGGCTGTCAACGGCTGATAGCTGAGTAATAAAGGTCCAAGGACCCAAAATCCGGGCTGCAGAAGCTCTGCCGTGGACTCTGTAc       <  1:333379/136‑1 (MQ=255)                                                                                                             ttttGTTATGTGTTGTCGTACAGCTATGGGTGATTATTAGCAAGGGGAAGATGGGCTGTCAACGGCTGATAGCTGAGTAATAAAGGTCCAAGGACCCAAAATCCGGGCTGCAGAAGCTCTGCCGTGGACTCTGTAc       <  2:1041010/136‑1 (MQ=255)                                                                                                                 gTTATGTGTTGTCGTACAGCTATGGGTGATTATTAGCAAGGGGAAGATGGGCTGTCAACGGCTGATAGCTGAGTAATAAAGGTCCAAGGACCCAAAATCCGGGCTGCAGAAGCTCTGCCGTGGACTCTGTACAgcc   >  1:737168/1‑134 (MQ=255)                                                                                                                  ttATGTGTTGTCGTACAGCTATGGGTGATTATTAGCAAGGGGAAGATGGGCTGTCAACGGCTGATAGCTGAGTAATAAAGGTCCAAGGACCCAAAATCCGGGCTGCAGAAGCTCTGCCGTGGACTCTGTACAGACa  <  2:1342043/136‑1 (MQ=255)                                                                                                                                   |                                                                                                                       GCGCTACGTACTGGCAGGCGTACGCGAGCTGGCCGGGGGAAGAGGGGGGGCAACAGGTGCGGGTGCAGCGGGATGGGTGTCGACATGGGTTCGTCCACGGCTTGTTGTTTTTGTTATGTGTTGTCGTACAACTATGGGTGATTATTAGCAAGGGGAAGATGGGCTGTCAACGGCTGATAGCTGAGTAATAAAGGTCCAAGGACCCAAAATCCGGGCTGCAGAAGCTCTGCCGTGGACTCTGTACAGACA  >  NC_002947/1348659‑1348907

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑