BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	508,774	Δ1 bp	6.7%	coding (240/1890 nt)	estP ←	esterase EstP

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	508,774	0	C	.	6.7%	681.2 / 43.8	208	coding (240/1890 nt)	estP	esterase EstP
Reads supporting (aligned to +/- strand): ref base C (120/73); new base . (9/5); total (130/78)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.45e-01

Only 100 of 211 total aligned reads displayed.

AACCCGGCATTGGGGTTTCCTGGGGGGATGACGGTCTCGGACGTTGTCGTGATCGAGTCCAGGATCTGCTGGGTGGTGTACCCGCCGACTGCCCAGTTATTACCATCGGGCTGGATACCTACGGATGTCGACGGGTTAAGGTCGCCTGGCGCGACGC‑CCAGGCGGCCACCGAGGATCATCGGCGACACCGGGGCGAAGTTGCCGTCGGCGTCACGGTTGGTGAAACGCGCGCCTGGGGTACCGCCAACAAGATCGGGAAACTGCCCGGCATCGGCGAGGCTGTCGCCAAACACGATC  >  NC_002947/508625‑508921

aaCCCGGCATTGGGGTTTCCTGGGGGGATGACGGTCTCGGACGTTGTCGTGATCGAGTCCAGGATCTGCTGGGTGGTGTACCCGCCGACTGCCCAGTTATTACCATCGGGCTGGATACCTACGGATGTCGACGGGTTAAGGTCGCCTGgc                                                                                                                                                      <  2:564232/150‑1 (MQ=255)

    cGGCATTGGGGTTTCCTGGGGGGATGACGGTCTCGGACGTTGTCGTGATCGAGTCCAGGATCTGCTGGGTGGTGTACCCGCCGACTGCCCAGTTATTACCATCGGGCTGGATACCTACGGATGTCGACGGGTTAAGGTCGCCTGGCGCGa                                                                                                                                                  >  1:5364125/1‑150 (MQ=255)

             ggTTTCCTGGGGGGATGACGGTCTCGGACGTTGTCGTGATCGAGTCCAGGATCTGCTGGGTGGTGTACCCGCCGACTGCCCAGTTATTACCATCGGGCTGGATACCTACGGATGTCGACGGGTTAAGGTCGCCTGGCGCGACGC‑CCAggc                                                                                                                                        <  2:3462487/150‑1 (MQ=255)

               tttCCTGGGGGGATGACGGTCTCGGACGTTGTCGTGATCGAGTCCAGGATCTGCTGGGTGGTGTACCCGCCGACTGCCCAGTTATTACCATCGGGCTGGATACCTACGGATGTCGACGGGTTAAGGTCGCCTGGCGCGACGC‑CCAggcgg                                                                                                                                      <  1:1175113/150‑1 (MQ=255)

               tttCCTGGGGGGATGACGGTCTCGGACGTTGTCGTGATCGAGTCCAGGATCTGCTGGGTGGTGTACCCGCCGACTGCCCAGTTATTACCATCGGGCTGGATACCTACGGATGTCGACGGGTTAAGGTCGCCTGGCGCGACGC‑CCAggcgg                                                                                                                                      >  1:278714/1‑150 (MQ=255)

               tttCCTGGGGGGATGACGGTCTCGGACGTTGTCGTGATCGAGTCCAGGATCTGCTGGGTGGTGTACCCGCCGACTGCCCAGTTATTACCATCGGGCTGGATACCTACGGATGTCGACGGGTTAAGGTCGCCTGGCGCGACGC‑CCAggcgg                                                                                                                                      <  1:934743/150‑1 (MQ=255)

                   cTGGGGGGATGACGGTCTCGGACGTTGTCGTGATCGAGTCCAGGATCTGCTGGGTGGTGTACCCGCCGACTGCCCAGTTATTACCATCGGGCTGGATACCTACGGATGTCGACGGGTTAAGGTCGCCTGG‑GCGTCGCGCCAggcg                                                                                                                                       >  2:1527723/1‑145 (MQ=255)

                   cTGGGGGGATGACGGTCTCGGACGTTGTCGTGATCGAGTCCAGGATCTGCTGGGTGGTGTACCCGCCGACTGCCCAGTTATTACCATCGGGCTGGATACCTACGGATGTCGACGGGTTAAGGTCGCCTGG‑GCGTCGCGCCAggcg                                                                                                                                       <  1:1527723/145‑1 (MQ=255)

                      gggggATGACGGTCTCGGACGTTGTCGTGATCGAGTCCAGGATCTGCTGGGTGGTGTACCCGCCGACTGCCCAGTTATTACCATCGGGCTGGATACCTACGGATGTCGACGGGTTAAGGTCGCCTGGCGCGACGC‑CCAGGCGGCCACCGa                                                                                                                               <  1:5430596/150‑1 (MQ=255)

                      gggggATGACGGTCTCGGACGTTGTCGTGATCGAGTCCAGGATCTGCTGGGTGGTGTACCCGCCGACTGCCCAGTTATTACCATCGGGCTGGATACCTACGGATGTCGACGGGTTAAGGTCGCCTGGCGCGACGC‑CCAGGCGGCCACCGa                                                                                                                               >  2:29075/1‑150 (MQ=255)

                      gggggATGACGGTCTCGGACGTTGTCGTGATCGAGTCCAGGATCTGCTGGGTGGTGTACCCGCCGACTGCCCAGTTATTACCATCGGGCTGGATACCTACGGATGTCGACGGGTTAAGGTCGCCTGGCGCGACGC‑CCAGGCGGCCACCGa                                                                                                                               <  1:1637816/150‑1 (MQ=255)

                      gggggATGACGGTCTCGGACGTTGTCGTGATCGAGTCCAGGATCTGCTGGGTGGTGTACCCGCCGACTGCCCAGTTATTACCATCGGGCTGGATACCTACGGATGTCGACGGGTTAAGGCCGCCTGG‑GCGTCGCGCCAGGCGAccttaac                                                                                                                               <  1:211805/150‑6 (MQ=255)

                      gggggATGACGGTCTCGGACGCTGTCGCGATCGAGTCCAGGAACCGCTGGGTGGGGTACCCGCCGACGGCCCAGTTATTCCCATCGGGCTGGATACCCACGGATGTCGACGGGTTCAGGTCGCCTGGCGCGACGC‑CCAGGCGGCCACCGa                                                                                                                               <  2:2555366/150‑1 (MQ=255)

                        gggATGACGGTCTCGGACGTTGTCGTGATCGAGTCCAGGATCTGCTGGGTGGTGTACCCGCCGACTGCCCAGTTATTACCATCGGGCTGGATACCTACGGATGTCGACGGGTTAAGGTCGCCTGGCGCGACGC‑CCAGGCGGCCACCGAgg                                                                                                                             <  1:2406161/150‑1 (MQ=255)

                        gggATGACGGTCTCGGACGTTGTCGTGATCGAGTCCAGGATCTGCTGGGTGGTGTACCCGCCGACTGCCCAGTTATTACCATCGGGCTGGATACCTACGGATGTCGACGGGTTAAGGTCGCCTGGCGCGACGC‑CCAGGCGGCCACCGAgg                                                                                                                             <  2:1230969/150‑1 (MQ=255)

                               cGGTCTCGGACGTTGTCGTGATCGAGTCCAGGATCTGCTGGGTGGTGTACCCGCCGACTGCCCAGTTATTACCATCGGGCTGGATACCTACGGATGTCGACGGGTTAAGGTCGCCTGGCGCGACGC‑CCAGGCGGCCACCGAGGATCATCg                                                                                                                      >  2:5142995/1‑150 (MQ=255)

                               cGGTCTCGGACGTTGTCGTGATCGAGTCCAGGATCTGCTGGGTGGTGTACCCGCCGACTGCCCAGTTATTACCATCGGGCTGGATACCTACGGATGTCGACGGGTTAAGGCCGCCTGGCGCGACGC‑CCAGGCGGCCACCGAGGATCATCg                                                                                                                      >  2:2916231/1‑150 (MQ=255)

                                ggTCTCGGACGTTGTCGTGATCGAGTCCAGGATCTGCTGGGTGGTGTACCCGCCGACTGCCCAGTTATTACCATCGGGCTGGATACCTACGGATGTCGACGGGTTAAGGTCGCCTGGCGCGACGC‑CCAGGCGGCCACCGAGGATCATCgg                                                                                                                     >  2:2165905/1‑150 (MQ=255)

                                 gTCTCGGACGTTGTCGTGATCGAGTCCAGGATCTGCTGGGTGGTGTACCCGCCGACTGCCCAGTTATTACCATCGGGCTGGATACCTACGGATGTCGACGGGTTAAGGTCGCCTGGCGCGACGC‑CCAGGCGGCCACCGAGGATCATCgac                                                                                                                    <  1:90268/150‑3 (MQ=255)

                                   ctcGGACGTTGTCGTGATCGAGTCCAGGATCTGCTGGGTGGTGTACCCGCCGACTGCCCAGTTATTACCATCGGGCTGGATACCTACGGATGTCGACGGGTTAAGGTCGCCTGGCGCGACGC‑CCAGGCGGCCACCGAGGATCATCGGCGa                                                                                                                  <  1:4757604/150‑1 (MQ=255)

                                      ggACGTTGTCGTGATCGAGTCCAGGATCTGCTGGGTGGTGTACCCGCCGACTGCCCAGTTATTACCATCGGGCTGGATACCTACGGATGTCGACGGGTTAAGGTCGCCTGGCGCGACGC‑CCAGGCGGCCACCGAGGATCATCGGCGacac                                                                                                               >  1:2950440/1‑150 (MQ=255)

                                        aCGTTGTCGTGATCGAGTCCAGGATCTGCTGGGTGGTGTACCCGCCGACTGCCCAGTTATTACCATCGGGCTGGATACCTACGGATGTCGACGGGTTAAGGTCGCCTGGCGCGACGC‑CCAGGCGGCCACCGAGGATCATCGGCGACACCg                                                                                                             >  1:3964325/1‑150 (MQ=255)

                                              tCGTGATCGAGTCCAGGATCTGCTGGGTGGTGTACCCGCCGACTGCCCAGTTATTACCATCGGGCTGGATACCTACGGATGTCGACGGGTTAAGGTCGCCTGGCGCGACGC‑CCAGGCGGCCACCGAGGATCATCGGCGACACCGGGGCGa                                                                                                       >  2:302971/1‑150 (MQ=255)

                                               cGTGATCGAGTCCAGGATCTGCTGGGTGGTGTACCCGCCGACTGCCCAGTTATTACCATCGGGCTGGATACCTACGGATGTCGACGGGTTAAGGTCGCCTGGCGCGACGC‑CCAGGCGGCCACCGAGGATCATCGGCGACACCGGGGCGaa                                                                                                      <  1:3538704/150‑1 (MQ=255)

                                                gTGATCGAGTCCAGGATCTGCTGGGTGGTGTACCCGCCGACTGCCCAGTTATTACCATCGGGCTGGATACCTACGGATGTCGACGGGTTAAGGTCGCCTGGCGCGACGC‑CCAGGCGGCCACCGAGGATCATCGGCGACACCGGGGCGAAg                                                                                                     >  2:3174046/1‑150 (MQ=255)

                                                   aTCGAGTCCAGGATCTGCTGGGTGGTGTACCCGCCGACTGCCCAGTTATTACCATCGGGCTGGATACCTACGGATGTCGACGGGTTAAGGTCGCCTGGCGCGACGC‑CCAGGCGGCCACCGAGGATCATCGGCGACACCGGGGCGAAGTTg                                                                                                  >  1:1984056/1‑150 (MQ=255)

                                                   aTCGAGTCCAGGATCTGCTGGGTGGTGTACCCGCCGACTGCCCAGTTATTACCATCGGGCTGGATACCTACGGATGTCGACGGGTTAAGGTCGCCTGGCGCGACGC‑CCAGGCGGCCACCGAGGATCATCGGCGACACCGGGGCGAAGTTg                                                                                                  >  2:88628/1‑150 (MQ=255)

                                                    tCGAGTCCAGGATCTGCTGGGTGGTGTACCCGCCGACTGCCCAGTTATTACCATCGGGCTGGATACCTACGGATGTCGACGGGTTAAGGTCGCCTGGCGCGACGC‑CCAGGCGGCCACCGAGGATCATCGGCGACACCGGGGCGAAGTTGc                                                                                                 >  2:2326991/1‑150 (MQ=255)

                                                      gAGTCCAGGATCTGCTGGGTGGTGTACCCGCCGACTGCCCAGTTATTACCATCGGGCTGGATACCTACGGATGTCGACGGGTTAAGGTCGCCTGGCGCGACGC‑CCAGGCGGCCACCGAGGATCATCGGCGACACCGGGGCGAAGTTGCCg                                                                                               >  2:316810/1‑150 (MQ=255)

                                                       aGTCCAGGATCTGCTGGGTGGTGTACCCGCCGACTGCCCAGTTATTACCATCGGGCTGGATACCTACGGATGTCGACGGGTTAAGGTCGCCTGGCGCGACGC‑CCAGGCGGCCACCGAGGATCATCGGCGACACCGGGGCGAAGTTGCCGt                                                                                              >  1:822114/1‑150 (MQ=255)

                                                         tCCAGGATCTGCTGGGTGGTGTACCCGCCGACTGCCCAGTTATTACCATCGGGCTGGATACCTACGGATGTCGACGGGTTAAGGTCGCCTGGCGCGACGC‑CCAGGCGGCCACCGAGGATCATCgg                                                                                                                     <  2:3579068/125‑1 (MQ=255)

                                                         tCCAGGATCTGCTGGGTGGTGTACCCGCCGACTGCCCAGTTATTACCATCGGGCTGGATACCTACGGATGTCGACGGGTTAAGGTCGCCTGGCGCGACGC‑CCAGGCGGCCACCGAGGATCATCGGCGACACCGGGGCGAAGTTGCCGTct                                                                                            >  1:4742388/1‑149 (MQ=255)

                                                         tCCAGGATCTGCTGGGTGGTGTACCCGCCGACTGCCCAGTTATTACCATCGGGCTGGATACCTACGGATGTCGACGGGTTAAGGTCGCCTGGCGCGACGC‑CCAGGCGGCCACCGAGGATCATCGGCGACACCGGGGCGAAGTTGCCGTCg                                                                                            >  1:378931/1‑150 (MQ=255)

                                                           cAGGATCTGCTGGGTGGTGTACCCGCCGACTGCCCAGTTATTACCATCGGGCTGGATACCTACGGATGTCGACGGGTTAAGGTCGCCTGGCGCGACGC‑CCAGGCGGCCACCGAGGATCATCGGCGACACCGGGGCGAAGTTGCCGTCGGc                                                                                          >  2:4913767/1‑150 (MQ=255)

                                                            aGGATCTGCTGGGTGGTGTACCCGCCGACTGCCCAGTTATTACCATCGGGCTGGATACCTACGGATGTCGACGGGTTAAGGTCGCCTGGCGCGACGC‑CCAGGCGGCCACCGAGGATCATCGGCGACACCGGGGCGAAGTTGCCGTCGGCg                                                                                         <  2:3478252/150‑1 (MQ=255)

                                                             ggATCTGCTGGGTGGTGTACCCGCCGACTGCCCAGTTATTACCATCGGGCTGGATACCTACGGATGTCGACGGGTTAAGGTCGCCTGGCGCGACGC‑CCAGGCGGCCACCAAGGATCATCGGCGACACCGGGGCGAAGTTGCCGTCGGCGt                                                                                        >  1:4504026/1‑150 (MQ=255)

                                                                  tgctgGGTGTTGTACCCGCCGACTGCCCAGTTATTACCATCGGGCTGGATACCTACGGATGTCGACGGGTTAAGTTCTCCTGGCGCGACGC‑CCAGGCGGCCACCGAGGATCATCGGCGACACCGTGGAGAAGTTGCCGTCGGCGTCACgg                                                                                   <  1:2604128/150‑1 (MQ=255)

                                                                  tgctgGGTGGTGTACCCGCCGACTGCCCAGTTATTACCATCGGGCTGGATACCTACGGATGTCGACGGGTTAAGGTCGCCTGGCGCGACGC‑CCAGGCGGCCACCGAGGATCATCGGCGACACCGGGGCGAAGTTGCCGTCGGCGTCACgg                                                                                   >  1:3282128/1‑150 (MQ=255)

                                                                   gctgGGTGGTGTACCCGCCGACTGCCCAGTTATTACCATCGGGCTGGATACCTACGGATGTCGACGGGTTAAGGTCGCCTGGCGCGACGC‑CCAGGCGGCCACCGAGGATCATCGGCGACACCGGGGCGAAGTTGCCGTCGGCGTCACGGt                                                                                  >  1:3671299/1‑150 (MQ=255)

                                                                   gctgGGTGGTGTACCCGCCGACTGCCCAGTTATTACCATCGGGCTGGATACCTACGGATGTCGACGGGTTAAGGTCGCCTGGCGCGACGC‑CCAGGCGGCCACCGAGGATCATCGGCGACACCGGGGCGAAGTTGCCGTCGGCGTCACGGt                                                                                  >  2:1088113/1‑150 (MQ=255)

                                                                   gctgGGTGGTGTACCCGCCGACTGCCCAGTTATTACCATCGGGCTGGATACCTACGGATGTCGACGGGTTAAGGTCGCCTGGCGCGACGC‑CCAGGCGGCCACCGAGGATCATCGGCGACACCGGGGCGAAGTTGCCGTCGGCGTCACGGt                                                                                  >  1:4853427/1‑150 (MQ=255)

                                                                       ggtggtGTACCCGCCGACTGCCCAGTTATTACCATCGGGCTGGATACCTACGGATGTCGACGGGTTAAGGTCGCCTGGCGCGACGC‑CCAGGCGGCCACCGAGGATCATCGGCGACACCGGGGCGAAGTTGCCGTCGGCGTCACGGTTGGt                                                                              >  2:4735462/1‑150 (MQ=255)

                                                                          ggtgTACCCGCCGACTGCCCAGTTATTACCATCGGGCTGGATACCTACGGATGTCGACGGGTTAAGGTCGCCTGGCGCGACGC‑CCAGGCGGCCACCGAGGATCATCGGCGACACCGGGGCGAAGTTGCCGTCGGCGTCACGGTTGGTGaa                                                                           <  1:302971/150‑1 (MQ=255)

                                                                                cccGCCGACTGCCCAGTTATTACCATCGGGCTGGATACCTACGGATGTCGACGGGTTAAGGTCGCCTGGCGCGACGC‑CCAGGCGGCCACCGAGGATCATCGGCGACACCGGGGCGAAGTTGCCGTCGGCGTCACGGTTGGTGAAAcgcgc                                                                     <  2:2786122/150‑1 (MQ=255)

                                                                                cccGCCGACTGCCCAGTTATTACCATCGGGCTGGATACCTACGGATGTCGACGGGTTAAGGTCGCCTGGCGCGACGC‑CCAGGCGGCCACCGAGGATCATCGGCGACACCGGGGCGAAGTTGCCGTCGGCGTCACGGTTGGTGAAAcgcgc                                                                     >  1:1897220/1‑150 (MQ=255)

                                                                                      gACTGCCCAGTTATTACCATCGGGCTGGATACCTACGGATGTCGACGGGTTAAGGTCGCCTGGCGCGACGC‑CCAGGCGGCCACCGAGGATCATCGGCGACACCGGGGCGAAGTTGCCGTCGGCGTCACGGTTGGTGAAACGCGCGCCTgg                                                               >  1:2256914/1‑150 (MQ=255)

                                                                                            ccAGTTATTACCATCGGGCTGGATACCTACGGATGTCGACGGGTTAAGGTCGCCTGGCGCGACGC‑CCAGGCGGCCACCGAGGATCATCGGCGACACCGGGGCGAAGTTGCCGTCGGCGTCACGGTTGGTGAAACGCGCGCCTGGGGTAcc                                                         <  2:2256914/150‑1 (MQ=255)

                                                                                            ccAGTTATTACCATCGGGCTGGATACCTACGGATGTCGACGGGTTAAGGTCGCCTGGCGCGACGC‑CCAGGCGGCCACCGAGGATCATCGGCGACACCGGGGCGAAGTTGCCGTCGGCGTCACGGTTGGTGAAACGCGCGCCTGGGGTAcc                                                         >  2:4025151/1‑150 (MQ=255)

                                                                                            ccAGTTATTACCATCGGGCTGGATACCTACGGATGTCGACGGGTTAAGGTCGCCTGGCGCGACGC‑CCAGGCGGCCACCGAGGATCATCGGCGACACCGGGGCGAAGTTGCCGTCGGCGTCACGGTTGGTGAAACGCGCGCCTGGGGTAcc                                                         <  1:4289990/150‑1 (MQ=255)

                                                                                            ccAGTTATTACCATCGGGCTGGATACCTACGGATGTCGACGGGTTAAGGTCGCCTGGCGCGACGC‑CCAGGCGGCCACCGAGGATCATCGGCGACACCGGGGCGAAGTTGCCGTCGGCGTCACGGTTGGTGAAACGCGCGCCTGGGGTAcc                                                         >  1:2928254/1‑150 (MQ=255)

                                                                                              aGTTATTACCATCGGGCTGGATACCTACGGATGTCGACGGGTTAAGGTCGCCTGGCGCGACGC‑CCAGGCGGCCACCGAGGATCATCGGCGACACCGGGGCGAAGTTGCCGTCGGCGTCACGGTTGGTGAAACGCGCGCCTGGGGTACCGc                                                       <  2:1259760/150‑1 (MQ=255)

                                                                                               gTTATTACCATCGGGCTGGATACCTACGGATGTCGACGGGTTAAGGTCGCCTGGCGCGACGC‑CCAGGCGGCCACCGAGGATCATCGGCGACACCGGGGCGAAGTTGCCGTCGGCGTCACGGTTGGTGAAACGCGCGCCTGGGGTACCGcc                                                      >  2:5263189/1‑150 (MQ=255)

                                                                                                ttattaCCATCGGGCTGGATACCTACGGATGTCGACGGGTTAAGGTCGCCTGGCGCGACGC‑CCAGGCGGCCACCGAGGATCATCGGCGACACCGGGGc                                                                                                         <  1:1302905/98‑1 (MQ=255)

                                                                                                ttattaCCATCGGGCTGGATACCTACGGATGTCGACGGGTTAAGGTCGCCTGGCGCGACGC‑CCAGGCGGCCACCGAGGATCATCGGCGACACCGGGGc                                                                                                         >  2:1302905/1‑98 (MQ=255)

                                                                                                  attaCCATCGGGCTGGATACCTACGGATGTCGACGGGTTAAGGTCGCCTGGCGCGACGC‑CCAGGCGGCCACCGAGGATCATCGGCGACACCGGGGCGAAGTTGCCGTCGGCGTCACGGTTGGTGAAACGCGCGCCTGGGGTACCGCcaac                                                   >  1:940284/1‑150 (MQ=255)

                                                                                                   ttaCCATCGGGCTGGATACCTACGGATGTCGACGGGTTAAGGTCGCCTGGCGCGACGC‑CCAGGCGGCCACCGAGGATCATCGGCGACACCGGGGCGAAGTTGCCGTCGGCGTCACGGTTGGTGAAACGCGCGCCTGGGGTACCGCcaaca                                                  <  2:1424068/150‑1 (MQ=255)

                                                                                                     aCCATCGGGCTGGATACCTACGGATGTCGACGGGTTAAGGTCGCCTGGCGCGACGC‑CCAGGCGGCCACCGAGGATCATCGGCGACACCGGGGCGAAGTTGCCGTCGGCGTCACGGTGGGTGAAACGCGCGCCTGGGGTACCGCCAACAAg                                                >  2:4599780/1‑150 (MQ=255)

                                                                                                      ccATCGGGCTGGATACCTACGGATGTCAACGGGTTAAGGTCGCCTGGCGCGACGC‑CCAGGCGGCCACCGAGGATCATCGGCGACACCGGGGCGAAGTTGCCGTCGGCGTCACGGTTGGTGAAACGCGCGCCTGGGGTACCGCCAACAAGa                                               >  1:2959345/1‑150 (MQ=255)

                                                                                                       cATCGGGCTGGATACCTACGGATGTCGACGGGTTAAGGTCGCCTGGCGCGACGC‑CCAGGCGGCCACCGAGGATCATCGGCGACACCGGGGCGAAGTTGCCGTCGGCGTCACGGTTGGTGaaa                                                                          <  2:1101443/122‑1 (MQ=255)

                                                                                                       cATCGGGCTGGATACCTACGGATGTCGACGGGTTAAGGTCGCCTGGCGCGACGC‑CCAGGCGGCCACCGAGGATCATCGGCGACACCGGGGCGAAGTTGCCGTCGGCGTCACGGTTGGTGaaa                                                                          >  1:1101443/1‑122 (MQ=255)

                                                                                                       cATCGGGCTGGATACCTACGGATGTCGACGGGTTAAGGTCGCCTGGCGCGACGC‑CCAGGCGGCCACCGAGGATCATCGGCGACACCGGGGCGAAGTTGCCGTCGGCGTCACGGTTGGTGAAACGCGCGCCTGGGGTACCGCCAACAAGAt                                              >  1:1153413/1‑150 (MQ=255)

                                                                                                       cATCGGGCTGGATACCTACGGATGTCGACGGGTTAAGGTCGCCTGGCGCGACGC‑CCAGGCGGCCACCGAGGATCATCGGCGACACCGGGGCGAAGTTGCCGTCGGCGTCACGGTTGGTGAAACGCGCGCCTGGGGTACCGCCAACAAGAt                                              >  2:4155462/1‑150 (MQ=255)

                                                                                                        aTCGGGCTGGATACCTACGGATGTCGACGGGTTAAGGTCGCCTGGCGCGACGC‑CCAGGCGGCCACCGAGGATCATCGGCGACACCGGGGCGAAGTTGCCGTCGGCGTCACGGTTGGTGAAACGCGCGCCTGGGGTACCGCCAACAAGATc                                             <  1:1003706/150‑1 (MQ=255)

                                                                                                         tCGGGCTGGATACCTACGGATGTCGACGGGTTAAGGTCGCCTGGCGCGACGC‑CCAGGCGGCCACCGAGGATCATCGGCGACACCGGGGCGAAGTTGCCGTCGGCGTCACGGTTGGTGAAACGCGCGCCTGGGGTACCGCCAACAAGATCg                                            <  1:600272/150‑1 (MQ=255)

                                                                                                          cGGGCTGGATACCTACGGATGTCGACGGGTTAAGGTCGCCTGGCGCGACGC‑CCAGGCGGCCACCGAGGATCATCGGCGACACCGGGGCGAAGTTGCCGTCGGCGTCACGGTTGGTGAAACGCGCGCCTGGGGTACCGCCAACAAGATCgg                                           <  2:4985032/150‑1 (MQ=255)

                                                                                                                  aTACCTACGGATGTCGACGGGTTAAGGTCGCCTGGCGCGACGC‑CCAGGCGGCCACCGAGGATCATCGGCGACACCGGGGCGAAGTTGCCGTCGGCGTCACGGTTGGTGAAACGCGCGCCTGGGGTACCGCCAACAAGATCGGGAAACTGc                                   >  1:2225448/1‑150 (MQ=255)

                                                                                                                  aTACCTACGGATGTCGACGGGTTAAGGTCGCCTGGCGCGACGC‑CCAGGCGGCCACCGAGGATCATCGGCGACACCGGGGCGAAGTTGCCGTCGGCGTCACGGTTGGTGAAACGCGCGCCTGGGGTACCGCCAACAAGATCGGGAAACTGc                                   >  1:3978789/1‑150 (MQ=255)

                                                                                                                     ccTACGGATGTCGACGGGTTAAGGTCGCCTGGCGCGACGC‑CCAGGCGGCCACCGAGGATCATCGGCGACACCGGGGCGAAGTTGCCGTCGGCGTCACGGTTGGTGAAACGCGCGCCTGGGGTACCGCCAACAAGATCGGGAAACTGCCCg                                <  2:5223161/150‑1 (MQ=255)

                                                                                                                      cTACGGATGTCGACGGGTTAAGGTCGCCTGGCGCGACGC‑CCAGGCGGCCACCGAGGATCATCGGCGACACCGGGGCGAAGTTGCCGTCGGCGTCACGGTTGGTGAAACGCGCGCCTGGGGTACCGCCAACAAGATCGGGAAACTGCCCgg                               >  2:674624/1‑150 (MQ=255)

                                                                                                                       tACGGATGTCGACGGGTTAAGGTCGCCTGGCGCGACGC‑CCAGGCGGCCACCGAGGATCATCGGCGACACCGGGGCGAAGTTGCCGTCGGCGTCACGGTTGGTGAAACGCGCGCCTGGGGTACCGCCAACAAGATCGGGAAACTGCCCGGc                              >  1:2722462/1‑150 (MQ=255)

                                                                                                                       tACGGATGTCGACGGGTTAAGGTCGCCTGGCGCGACGC‑CCAGGCGGCCACCGAGGATCATCGGCGACACCGGGGCGAAGTTGCCGTCGGCGTCACGGTTGGTGAAACGCGCGCCTGGGGTACCGCCAACAAGATCGGGAAACTGCCCGGc                              >  2:4325830/1‑150 (MQ=255)

                                                                                                                       tACGGATGTCGACGGGTTAAGGTCGCCTGGCGCGACGC‑CCAGGCGGCCACCGAGGATCATCGGCGACACCGGGGCGAAGTTGCCGTCGGCGTCACGGTTGGTGAAACGCGCGCCTGGGGTACCGCCAACAAGATCGGGAAACTGCCCGGc                              >  1:3382755/1‑150 (MQ=255)

                                                                                                                          ggATGTCGACGGGTTAAGGTCGCCTGGCGCGACGC‑CCAGGCGGCCACCGAGGATCATCGGCGACACCGGGGCGAAGTTGCCGTCGGCGTCACGGTTGGTGAAACGCGCGCCTGGGGTACCGCCAACAAGATCGGGAAACTGCCCGGCATc                           >  1:283560/1‑150 (MQ=255)

                                                                                                                           gATGTCGACGGGTTAAGGTCGCCTGGCGCGACGC‑CCAGGCGGCCACCGAGGATCATCGGCGACACCGGGGCGAAGTTGCCGTCGGCGTCACGGTTGGTGAAACGCGCGCCTGGGGTACCGCCAACAAGATCGGGAAACTGCCCGGCATCg                          <  2:3697959/150‑1 (MQ=255)

                                                                                                                               tCGACGGGTTAAGGTCGCCTGGCGCGACGC‑CCAGGCGGCCACCGAGGATCATCGGCGACACCGGGGCGAAGTTGCCGTCGGCGTCACGGTTGGTGAAACGCGCGCCTGGGGTACCGCCAACAAGATCGGGAAACTGCCCGGCATCGGCGa                      >  2:5118165/1‑150 (MQ=255)

                                                                                                                               tCGACGGGTTAAGGTCGCCTGGCGCGACGC‑CCAGGCGGCCACCGAGGATCATCGGCGACACCGGGGCGAAGTTGCCGTCGGCGTCACGGTTGGTGAAACGCGCGCCTGGGGTACCGCCAACAAGATCGGGAAACTGCCCGGCATCGGCGa                      <  1:4961700/150‑1 (MQ=255)

                                                                                                                                cGACGGGTTAAGGTCGCCTGGCGCGACGC‑CCAGGCGGCCACCGAGGATCATCGGCGACACCGGGGCGAAGTTGCCGTCGGCGTCACGGTTGGTGAAACGCGCGCCTGGGGTACCGCCAACAAGATCGGGAAACTGCCCGGCATCGGCGAg                     <  2:360778/150‑1 (MQ=255)

                                                                                                                                 gACGGGTTAAGGTCGCCTGGCGCGACGC‑CCAGGCGGCCACCGAGGATCATCGGCGACACCGGGGCGAAGTTGCCGTCGGCGTCACGGTTGGTGAAACGCGCGCCTGGGGTACCGCCAACAAGATCGGGAAACTGCCCGGCATCGGCGAgg                    >  1:4376479/1‑150 (MQ=255)

                                                                                                                                 gACGGGTTAAGGTCGCCTGGCGCGACGC‑CCAGGCGGCCACCGAGGATCATCGGCGACACCGGGGCGAAGTTGCCGTCGGCGTCACGGTTGGTGAAACGCGCGCCTGGGGTACCGCCAACAAGATCGGGAAACTGCCCGGCATCGGCGAgg                    >  2:1839982/1‑150 (MQ=255)

                                                                                                                                 gACGGGTTAAGGTCGCCTGGCGCGACGC‑CCAGGCGGCCACCGAGGATCATCGGCGACACCGGGGCGAAGTTGCCGTCGGCGTCACGGTTGGTGAAACGCGCGCCTGGGGTACCGCCAACAAGATCGGGAAACTGCCCGGCATCGGCGAgg                    >  1:942257/1‑150 (MQ=255)

                                                                                                                                  aCGGGTTAAGGTCGCCTGGCGCGACGC‑CCAGGTGGCCACCGAGGATCATCGGCGACACCGGGGCGAAGTTGCCGTCGGCGTCACGGTTGGTGAAACGCGCGCCTGGGGTACCGCCAACAAGATCGGGAAACTGCCCGGCATCGGCGAGGc                   >  2:1732499/1‑150 (MQ=255)

                                                                                                                                    gggTTAAGGTCGCCTGGCGCGACGC‑CCAGGCGGCCACCGAGGATCATCGGCGACACCGGGGCGAAGTTGCCGTCGGCGTCACGGTTGGTGAAACGCGCGCCTGGGGTACCGCCAACAAGATCGGGAAACTGCCCGGCATCGGCGAGGCTg                 >  1:2637795/1‑150 (MQ=255)

                                                                                                                                      gTTAAGGTCGCCTGGCGCGACGC‑CCAGGCGGCCACCGAGGATCATCGGCGACACCGGGGCGAAGTTGCCGTCGGCGTCACGGTTGGTGAAACGCGCGCCTGGGGTACCGCCAACAAGATCGGGAAACTGCCCGGCATCGGCGAGGCTGTc               >  1:3843694/1‑150 (MQ=255)

                                                                                                                                      gTTAAGGTCGCCTGGCGCGACGC‑CCAGGCGGCCACCGAGGATCATCGGCGACACCGGGGCGAAGTTGCCGTCGGCGTCACGGTTGGTGAAACGCGCGCCTGGGGTACCGCCAACAAGATCGGGAAACTGCCCGGCATCGGCGAGGCTGTc               >  1:3167896/1‑150 (MQ=255)

                                                                                                                                       ttAAGGTCGCCTGGCGCGACGC‑CCAGGCGGCCACCGAGGATCATCGGCGACACCGGGGCGAAGTTGCCGTCGGCGTCACGGTTGGTGAAACGCGCGCCTGGGGTACCGCCAACAAGATCGGGAAACTGCCCGGCATCGGCGAGGCTGTCg              >  2:2471982/1‑150 (MQ=255)

                                                                                                                                         gtggccGCCTGG‑GCGTCGCGCCAGGCGACCACCGAGGATCATCGGCGACACCGGGGCGAAGTTGCCGTCGGCGTCACGGTTGGTGAAACGCGCGCCTGGGGTACCGCCAACAAGATCGGGAAACTGCCCGGCATCGGCGAGGCTGTCGcc            >  1:3919046/6‑150 (MQ=255)

                                                                                                                                          aGGTCGCCTGGCGCGACGC‑CCAGGCGGCCACCGAGGATCATCGGCGACACCGGGGCGAAGTTGCCGTCGGCGTCACGGTTGGTGAAACGCGCGCCTGGGGTACCGCCAACAAGATCGGGAAACTGCCCGGCATCGGCGAGGCTGTCGCCa           >  1:5379983/1‑150 (MQ=255)

                                                                                                                                           ggTCGCCTGGCGCGACGC‑CCAGGCGGCCACCGAGGATCATCGGCGACACCGGGGCGAAGTTGCCGTCGGCGTCACGGTTGGTGAAACGCGCGCCTGGGGTACCGCCAACAAGATCGGGAAACTGCCCGGCATCGGCGAGGCTGTCGCCaa          >  1:4178276/1‑150 (MQ=255)

                                                                                                                                           ggTCGCCTGGCGCGACGC‑CCAGGCGGCCACCGAGGATCATCGGCGACACCGGGGCGAAGTTGCCGTCGGCGTCACGGTTGGTGAAACGCGCGCCTGGGGTACCGCCAACAAGATCGGGAAACTGCCCGGCATCGGCGAGGCTGTCGCCaa          >  1:4562336/1‑150 (MQ=255)

                                                                                                                                            gccGCCTGG‑GCGTCGCGCCAGGCGGCCACCGAGGATCATCGGCGACACCGGGGCGAAGTTGCCGTCGGCGTCACGGTTGGTGAAACGCGCGCCTGGGGTACCGCCAACAAGATCGGGAAACTGCCCGGCATCGGCGAGGCTGTCGCCaaa         >  1:249435/3‑150 (MQ=255)

                                                                                                                                            gccGCCTGG‑GCGTCGCGCCAGGCGACCACCGAGGATCATCGGCGACACCGGGGCGAAGTTGCCGTCGGCGTCACGGTTGGTGAAACGCGCGCCTGGGGTACCGCCAACAAGATCGGGaa                                        <  2:3357685/117‑1 (MQ=255)

                                                                                                                                            gTCGCCTGGCGCGACGC‑CCAGGCGGCCACCGAGGATCATCGGCGACACCGGGGCGAAGTTGCCGTCGGCGTCACGGTTGGTGAAACGCGCGCCTGGGGTACCGCCAACAAGATCGGGAAACTGCCCGGCATCGGCGAGGCTGTCGCCaaa         >  2:4261920/1‑150 (MQ=255)

                                                                                                                                            gTCGCCTGGCGCGACGC‑CCAGGCGGCCACCGAGGATCATCGGCGACACCGGGGCGAAGTTGCCGTCGGCGTCACGGTTGGTGAAACGCGCGCCTGGGGTACCGCCAACAAGATCGGGAAACTGCCCGGCATCGGCGAGGCTGTCGCCaaa         >  2:1574999/1‑150 (MQ=255)

                                                                                                                                            gTCGCCTGGCGCGACGC‑CCAGGCGGCCACCGAGGATCATCGGCGACACCGGGGCGAAGTTGCCGTCGGCGTCACGGTTGGTGAAACGCGCGCCTGGGGTACCGCCAACAAGATCGGGAAACTGCCCGGCATCGGCGAGGCTGTCGCCaaa         >  1:5083906/1‑150 (MQ=255)

                                                                                                                                             tCGCCTGGCGCGACGC‑CCAGGCGGCCACCGAGGATCATCGGCGACACCGGGGCGAAGTTGCCGTCGGCGTCACGGTTGGTGAAACGCGCGCCTGGGGTACCGCCAACAAGATCGGGAAACTGCCCGGCATCGGCGAGGCTGTCGCCAAac        <  2:1997962/150‑1 (MQ=255)

                                                                                                                                             tCGCCTGGCGCGACGC‑CCAGGCGGCCACCGAGGATCATCGGCGACACCGGGGCGAAGTTGCCGTCGGCGTCACGGTTGGTGAAACGCGCGCCTGGGGTACCGCCAACAAGATCGGGAAACTGCCCGGCATCGGCGAGGCTGTCGCCAAac        <  2:5364125/150‑1 (MQ=255)

                                                                                                                                             tCGCCTGGCGCGACGC‑CCAGGCGGCCACCGAGGATCATCGGCGACACCGGGGCGAAGTTGCCGTCGGCGTCACGGTTGGTGAAACGCGCGCCTGGGGTACCGCCAACAAGATCGGGAAACTGCCCGGCATCGGCGAGGCTGTCGCCAAac        >  1:4411228/1‑150 (MQ=255)

                                                                                                                                              cGCCTGGCGCGACGC‑CCAGGCGGCCACCGAGGATCATCGGCGACACCGGGGCGAAGTTGCCGTCGGCGTCACGGTTGGTGAAACGCGCGCCTGGGGTACCGCCAACAAGATCGGGAAACTGCCCGGCATCGGCGAGGCTGTCGCCAAaca       >  1:227819/1‑150 (MQ=255)

                                                                                                                                              cGCCTGG‑GCGTCGCGCCAGGCGGTCACCGAGGATCATCGGCGACACCGGGGCGAAGTTGCCGTCGGCGTCACGGTTGGTGAAACGCGCGCCTGGGGTACCGCCAACAAGATCGGGAAACTGCCCGGCATCGGCGAGGCTGTCGCCAAaca       >  2:2842258/1‑150 (MQ=255)

                                                                                                                                                   ggCGCGACGC‑CCAGGCGGCCACCGAGGATCATCGGCGACACCGGGGCGAAGTTGCCGTCGGCGTCACGGTTGGTGAAACGCGCGCCTGGGGTACCGCCAACAAGATCGGGAAACTGCCCGGCATCGGCGAGGCTGTCGCCAAACACGATc  >  1:3015791/1‑150 (MQ=255)

AACCCGGCATTGGGGTTTCCTGGGGGGATGACGGTCTCGGACGTTGTCGTGATCGAGTCCAGGATCTGCTGGGTGGTGTACCCGCCGACTGCCCAGTTATTACCATCGGGCTGGATACCTACGGATGTCGACGGGTTAAGGTCGCCTGGCGCGACGC‑CCAGGCGGCCACCGAGGATCATCGGCGACACCGGGGCGAAGTTGCCGTCGGCGTCACGGTTGGTGAAACGCGCGCCTGGGGTACCGCCAACAAGATCGGGAAACTGCCCGGCATCGGCGAGGCTGTCGCCAAACACGATC  >  NC_002947/508625‑508921

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`