| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 85,091 | Δ1 bp | 6.2% | intergenic (+69/+61) | aroE → / ← PP_0075 | shikimate 5‑dehydrogenase/choline sulfate transporter |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 85,091 | 0 | A | . | 6.2% | 432.8 / 24.7 | 145 | intergenic (+69/+61) | aroE/PP_0075 | shikimate 5‑dehydrogenase/choline sulfate transporter |
| Reads supporting (aligned to +/- strand): ref base A (62/70); new base . (2/7); total (68/77) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.83e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.35e-01 | |||||||||||
Only 100 of 148 total aligned reads displayed.
GCCTTCTTTATCTGGCGTGGCGTGAGGCCGGATACGGCGCCGGTGTTGGCTGAACTGCGCCGGCAACTCGCTCGGGGCTGAGCTGGCCGGGGCCGCTTGGCGGCCCTATCGCGACGCAAGGCCGTTCCCACAAAGTA‑CGTCGTACGCCGATCCTCTGTGGGAGCGGCCTTGCGTCGCGAT‑GGGCTGCAAAGCAGACCCAGAATCCCAACCTCATTCCTCAAACCGGATCGGGCACAACTCCACCCCTTCCAGCTTCTGCAACTCCTCGATCACCTGCGGCCTGGCCCGGTGCAAGGT > NC_002947/84942‑85238 | ccTTCTTTATCTGGCGTGGCGTGAGGCCGGATACGGCGCCGGTGTTGGCTGAACTGCGCCGGCAACTCGCTCGGGGCTGAGCTGGCCGGGGCCGCTTGGCGGCCCTATCGCGACGCAAGGCCGTTCCCACAAAGTA‑CGTCGTACGCCGAt > 1:1140699/1‑150 (MQ=255) ttcttTATCTGGCGTGGCGTGAGGCCGGATACGGCGCCGGTGTTGGCTGAACTGCGCCGGCAACTCGCTCGGGGCTGAGCTGGCCGGGGCCGCTTGGCGGCCCTATCGCGACGCAAGGCCGTTCCCCCAAAGTA‑CGGCGGACGCCGATcc > 1:1581410/1‑150 (MQ=255) ttcttTATCTGGCGTGGCGTGAGGCCGGATACGGCGCCGGTGTTGGCTGAACTGCGCCGGCAACTCGCTCGGGGCTGAGCTGGCCGGGGCCGCTTGGCGGCCCTATCGCGACGCAAGGCCGTTCCCACAAAGTA‑CGTCGTACGCCGATcc > 1:1127699/1‑150 (MQ=255) ttcttTATCTGGCGTGGCGTGAGGCCGGATACGGCGCCGGTGTTGGCTGAACTGCGCCGGCAACTCGCTCGGGGCTGAGCTGGCCGGGGCCGCTTGGCGGCCCTATCGCGACGCAAGGCCGTTCCCACAAAGTA‑CGTCGTACGCCGATcc > 2:3827074/1‑150 (MQ=255) tttATCTGGCGTGGCGTGAGGCCGGATACGGCGCCGGTGTTGGCTGAACTGCGCCGGCAACTCGCTCGGGGCTGAGCTGGCCGGGGCCGCTTGGCGGCCCTATCGCGACGCAAGGCCGTTCCCACAAAGTA‑CGTCGTACGCCGATCctct < 2:91216/150‑1 (MQ=255) tttATCTGGCGTGGCGTGAGGCCGGATACGGCGCCGGTGTTGGCTGAACTGCGCCGGCAACTCGCTCGGGGCTGAGCTGGCCGGGGCCGCTTGGCGGCCCTATCGCGACGCAAGGCCGTTCCCACAAAGTA‑CGTCGTACGCCGATCctct > 2:909030/1‑150 (MQ=255) tttATCTGGCGTGGCGTGAGGCCGGATACGGCGCCGGTGTTGGCTGAACTGCGCCGGCAACTCGCTCGGGGCTGAGCTGGCCGGGGCCGCTTGGCGGCCCTATCGCGACGCAAGGCCGTTCCCACAAAGTA‑CGTCGTACGCCGATCctct > 1:434548/1‑150 (MQ=255) tATCTGGCGTGGCGTGAGGCCGGATACGGCGCCGGTGTTGGCTGAACTGCGCCGGCAACTCGCTCGGGGCTGAGCTGGCCGGGGCCGCTTGGCGGCCCTATCGCGACGCAAGGCCGTTCCCACAAAGTA‑CGTCGTACGCCGATCCTCtgt < 2:5045522/150‑1 (MQ=255) aTCTGGCGTGGCGTGAGGCCGGATACGGCGCCGGTGTTGGCTGAACTGCGCCGGCAACTCGCTCGGGGCTGAGCTGGCCGGGGCCGCTTGGCGGCCCTATCGCGACGCCAGGCCGGTGCCACAAAATA‑CGTAGGAAGCCGAACCACagtg > 1:7702/1‑150 (MQ=255) aTCTGGCGTGGCGTGAGGCCGGATACGGCGCCGGTGTTGGCTGAACTGCGCCGGCAACTCGCTCGGGGCTGAGCTGGCCGGGGCCGCTTGGCGGCCCTATCGCGACGCAAGGCCGTTCCCACAAAGTA‑CGTCGTACGCCGATCCTCtgtg < 1:4360930/150‑1 (MQ=255) aTCTGGCGTGGCGTGAGGCCGGATACGGCGCCGGTGTTGGCTGAACTGCGCCGGCAACTCGCTCGGGGCTGAGCTGGCCGGGGCCGCTTGGCGGCCCTATCGCGACGCAAGGCCGTTCCCACAAAGTA‑CGTCGTACGCCGATCCTCtgtg < 1:1454028/150‑1 (MQ=255) aTCTGGCGTGGCGTGAGGCCGGATACGGCGCCGGTGTTGGCTGAACTGCGCCGGCAACTCGCTCGGGGCTGAGCTGGCCGGGGCCGCTTGGCGGCCCTATCGCGACGCAAGGCCGTTCCCACAAAGTA‑CGTCGTACGCCGATCCTCtgtg < 1:909030/150‑1 (MQ=255) tCTGGCGTGGCGTGAGGCCGGATACGGCGCCGGTGTTGGCTGAACTGCGCCGGCAACTCGCTCGGGGCTGAGCTGGCCGGGGCCGCTTGGCGGCCCTATCGCGACGCAAGGCCGTTCCCACAAAGTA‑CGTCGTACGCCGATCCTCTGTgg < 1:478751/150‑1 (MQ=255) cTGGCGTGGCGTGAGGCCGGATACGGCGCCGGTGTTGGCTGAACTGCGCCGGCAACTCGCTCGGGGCTGAGCTGGCCGGGGCCGCTTGGCGGCCCTATCGCGACGCAAGGCCGTTCCCACAAAGTA‑CGTCGTACGCCGATCCTCTGTggg < 1:2843121/150‑1 (MQ=255) ggcgtggcgtGAGGCCGGATACGGCGCCGGTGTTGGCTGAACTGCGCCGGCAACTCGCTCGGGGCTGAGCTGGCCGGGGCCGCTTGGCGGCCCTATCGCGACGCAAGGCCGTTCCCACAAAGTA‑CGTCGTACGCCGATCCTCTGTGGGAg < 1:1746660/150‑1 (MQ=255) gcgtggcgtgAGGCCGGATACGGCGCCGGTGTTGGCTGAACTGCGCCGGCAACTCGCTCGGGGCTGAGCTGGCCGGGGCCGCTTGGCGGCCCTATCGCGACGCAAGGCCGCTCCCACAGAGGATCGGCGTACGACG‑TACTTTGTGGGaac < 2:412653/150‑3 (MQ=25) cgtggcgtgAGGCCGGATACGGCGCCGGTGTTGGCTGATCTGCGCCGGCAACTCGCTCGGGGCTGAGCTGGCCGGGGCCGCTTGGCGGCCCTATCGCGACGCAAGGCCGCTCCCACAGAGGATCGGCGTACGACG‑TACTTTGTGGGAACg < 1:4171928/150‑1 (MQ=21) cgtggcgtgAGGCCGGATACGGCGCCGGTGTTGGCTGAACTGCGCCGGCAACTCGCTCGGGGCTGAGCTGGCCGGGGCCGCTTGGCGGCCCTATCGCGACGCAAGGCCGTTCCCACAAAGTA‑CGTCGTACGCCGATCCTCTGTGGGAGCg < 2:1351914/150‑1 (MQ=255) cgtggcgtgAGGCCGGATACGGCGCCGGTGTTGGCTGAACTGCGCCGGCAACTCGCTCGGGGCTGAGCTGGCCGGGGCCGCTTGGCGGCCCTATCGCGACGCAAGGCCGCTCCCACAGAGGATCGGCGTACGACG‑TACTTTGTGGGAACg < 2:1253563/150‑1 (MQ=21) gtggcgtgAGGCCGGATACGGCGCCGGTGTTGGCTGAACTGCGCCGGCAACTCGCTCGGGGCTGAGCTGGCCGGGGCCGCTTGGCGGCCCCATCGCGACGCAAGGCCGCTCCCACAGAGGATCGGCGTACGACG‑TACTTTGTGGGAACgg < 1:2580480/150‑1 (MQ=21) tggcgtgAGGCCGGATACGGCGCCGGTGTTGGCTGAACTGCGCCGGCAACTCGCTCGGGGCTGAGCTGGCCGGGGCCGCTTGGCGGTCCTATCGCGACGCAAGGCCGTTCCCACAAAGTA‑CGTCGTACGCCGATCCTCTGTGGGAGCGGc < 2:4439871/150‑1 (MQ=255) tggcgtgAGGCCGGATACGGCGCCGGTGTTGGCTGAACTGCGCCGGCAACTCGCTCGGGGCTGAGCTGGCCGGGGCCGCTTGGCGGCCCTATCGCGACGCAAGGCCGTTCCCACAAAGTA‑CGTCGTACGCCGATCCTCTGTGGGAGCGGc < 2:3165774/150‑1 (MQ=255) gcgtgAGGCCGGATACGGCGCCGGTGTTGGCTGAACTGCGCCGGCAACTCGCTCGGGGCTGAGCTGGCCGGGGCCGCTTGGCGGCCCTATCGCGACGCAAGGCCGTTCCCACAAAGTA‑CGTCGTACGCCGATCCTCTGTGGGAGCGGCCt > 1:3150277/1‑150 (MQ=255) cgtgAGGCCGGATACGGCGCCGGTGTTGGCTGAACTGCGCCGGCAACTCGCTCGGGGCTGAGCTGGCCGGGGCCGCTTGGCGGCCCTATCGCGACGCAAGGCCGTTCCCACAAAGTA‑CGTCGTACGCCGATCCTCTGTGGGAGCGGCCtt < 2:2774574/150‑1 (MQ=255) tgAGGCCGGATACGGCGCCGGTGTTGGCTGAACTGCGCCGGCAACTCGCTCGGGGCTGAGCTGGCCGGGGCCGCTTGGCGGCCCTATCGCGACGCAAGGCCGTTCCCACAAAGTA‑CGTCGTACGCCGATCCTCTGTGGGAGCGGCCTTGc < 1:742045/150‑1 (MQ=255) gCCGGATACGGCGCCGGTGTTGGCTGAACTGCGCCGGCAACTCGCTCGGGGCTGAGCTGGCCGGGGCCGCTTGGCGGCCCTATCGCGACGCAAGGCCGTTCCCACAAAGTA‑CGGCGTACGCCGATCCTCTGTGGGAGCGGCCTTgcggcg > 2:2410153/1‑150 (MQ=255) ccGGATATGGCGCCGGTGTTGGCTGAACTGCGCCGGCAACTCGCTCGGGGGTGAGCTGGCCGGGGCCGCTTGGCGGCCCTATCGCGACGCAAGGCCGTTCCCACAAAGTA‑CGTCGTACGCCGATCCTCTGTGGGAGCGGCCTTGCGTcgc < 1:1908616/150‑1 (MQ=255) ccGGATACGGCGCCGGTGTTGGCTGAACTGCGCCGGCAACTCGCTCGGGGCTGAGCTGGCCGGGGCCGCTTGGCGGCCCTATCGCGACGCAAGGCCGTTCCCACAAAGTA‑CGTCGTACGCCGATCCTCTGTGGGAGCGGCCTTGCGTcgc < 2:4430795/150‑1 (MQ=255) ggATACGGCGCCGGTGTTGGCTGAACTGCGCCGGCAACTCGCTCGGGGCTGAGCTGGCCGGGGCCGCTTGGCGGCCCTATCGCGACGCAAGGCCGGTACCACAAAGTA‑CGTCGTACGCCGATCCACAGTGGGAGCGGCCTTGCGTCGCGa > 1:1002917/1‑150 (MQ=255) gATACGGCGCCGGTGTTGGCTGAACTGCGCCGGCAACTCGCTCGGGGCTGAGCTGGCCGGGGCCGCTTGGCGGCCCTATCGCGACGCAAGGCCGTTCCCACAAAGTA‑CGTCGTACGCCGATCCTCTGTGGGAGCGGCCTTGCGTCGCGAt < 2:447945/150‑1 (MQ=255) gATACGGCGCCGGTGTTGGCTGAACTGCGCCGGCAACTCGCTCGGGGCTGAGCTGGCCGGGGCCGCTTGGCGGCCCTATCGCGACGCAAGGCCGTTCCCACAAAGTA‑CGTCGTACGCCGATCCTCTGTGGGAGCGGCCTTGCGTCGCGAt < 2:3546003/150‑1 (MQ=255) gATACGGCGCCGGTGTTGGCTGAACTGCGCCGGCAACTCGCTCGGGGCTGAGCTGGCCGGGGCCGCTTGGCGGCCCTATCGCGACGCAAGGCCGTTCCCACAAAGTA‑CGTCGTACGCCGATCCTCTGTGGGAGCGGCCTTGCGTCGCGAt < 2:4733955/150‑1 (MQ=255) gATACGGCGCCGGTGTTGGCTGAACGGCGCCGGCAACTCGCTCGGGGCTGAGCTGGCCGGGGCCGCTTGGCGGCCCTATCGCGACGCAAGGCCGTTCCCACAAAGTA‑CGTCGTACGCCGATCCTCTGTGGGAGCGGCCTTGCGTCGCGAt < 2:4909628/150‑1 (MQ=255) tACGGCGCGGGTGTTGGCTGAACTGCGCCGGCAACTCGCTCGGGGCTGAGCTGGCGGGGGCCGCTTGGCGGCCCTATCGCGACGCAAGGCCGCTCCAACAGAGGATCGGCTTACGACG‑TACTTTGTGGGAACGGCCTTGCGTCGCGAtag < 1:1766584/150‑3 (MQ=17) ggCGCCGGTGTTGGCTGAACTGCGCCGGCAACTCGCTCGGGGCTGAGCTGGCCGGGGCCGCTTTGCAGCCC‑ATCGCGACGCAAGGCCGTTCCCACAAAGTA‑CGTCGTACGCCGATCCTCtgt > 1:2420683/1‑122 (MQ=255) ggCGCCGGTGTTGGCTGAACTGCGCCGGCAACTCGCTCGGGGCTGAGCTGGCCGGGGCCGCTTGGCGGCCCTATCGCGACGCAAGGCCGTTCCCACAAAGTA‑CGTCGTACGCCGATCCTCTGTGGGAGCGGCCTTGCGTCGCGAT‑GGGCt > 2:1096102/1‑150 (MQ=255) cGGTGTTGGCTGAACTGCGCCGGCAACTCGCTCGGGGCTGAGCTGGCCGGGGCCGCTTGGCGGCCCTATCGCGACGCAAGGCCGTTCCCTCAAAGTA‑CGTCGTACGCCGATCCTCTGTGGGAGCGGCCTTGCGTCGCGAT‑GGGCTGCaaa < 2:2812721/150‑1 (MQ=255) ggTGTTGGCTGAACTGCGCCGGCAACTCGCTCGGGGCTGAGCTGGCCGGGGCCGCTTGGCGGCCCTATCGCGACGCAAGGCCGCTCCCACAGAGGATCGGCGTACGACG‑TACTTTGTGGGAACGGc < 2:3581858/126‑1 (MQ=18) ggTGTTGGCTGAACTGCGCCGGCAACTCGCTCGGGGCTGAGCTGGCCGGGGCCGCTTGGCGGCCCTATCGCGACGCAAGGCCGCTCCCACAGAGGATCGGCGTACGACG‑TACTTTGTGGGAACGGc > 1:3581858/1‑126 (MQ=18) ggTGTTGGCTGAACTGCGCCGGCAACGCGCTCGGGGCTGCGCTGGCGGGGGCCGATTGGCGGCCATAGCGCGACGCACGGCGGTTCCCACAAAGTA‑GGGCGTACGCCGATCCTCTGGGGTAGCGGCCTTGCGTCGCGAT‑GGGCTGCAAAg < 2:1832082/150‑1 (MQ=255) ttGGCTGAACTGCGCCGGCAACTCGCTCGTGGCTGAGCTGGCCGGGGCCGCTTGGCGGCCCTATCGCGACGCAAGGCCGTTCCCACAAAGTA‑CGTCGTACGCCGATCCTCTGTGGGAGCGGCCTTGCGTCGCGAT‑GGGCTGCAAAGCAGa > 2:3893051/1‑150 (MQ=255) ttGGCTGAACTGCGCCGGCAACTCGCTCGGGGCTGAGCTGGCCGGGGCCGCTTGGCGGCCCTATCGCGACGCAAGGCCGTTCCCACAAAGTA‑CGTCGTACGCCGATCCTCTGTGGGAGCGGCCTTGCGTCGCGAT‑GGGCTGCAAAGCAGa > 1:2700542/1‑150 (MQ=255) tGGCTGAACTGCGCCGGCAACTCGCTCGGGGCTGAGCTGGCCGGGGCCGCTTGGCGGCCCTATCGCGACGCAAGGCCGTTCCCACAAAGTA‑CGTCGTACGCCGATCCTCTGTGGGAGCGGCCTTGCGTCGCGAT‑GGGCTGCAAAGCAGAc < 2:4248312/150‑1 (MQ=255) gCTGAACTGCGCCGGCAACTCGCTCGGGGCTGAGCTGGCCGGGGCCGCTTGGCGGCCCTATCGCGACGCCAGGCCGGTGCCACAAAATA‑AGGCGTAACCCCATACAAAGTGGGGGCGGGCATTCGTCGCGAT‑GGGCAGCAAAGCAGAccc > 1:2442240/1‑150 (MQ=255) gCTGAACTGCGCCGGCAACTCGCTCGGGGCTGAGCTGGCCGGGGCCGCTTGGCGGCCCTATCGCGACGCAAGGCCGTTCCCACAAAGTA‑CGTCGTACGCCGATCCTCTGTGGGAGCGGCCTTGCGTCGCGAT‑GGGCTGCAAAg < 2:3171568/143‑1 (MQ=255) tGAACTGCGCCGGCAACTCGCTCGGGGCTGAGCTGGCCGGGGCCGCTTGGCGGCCCTATCGCGACGCAAGGCCGTTCCCACAAAGTA‑CGTCGTACGCCGAt < 2:4364310/101‑1 (MQ=255) cTGCGCCGGCAACTCGCTCGGGGCTGAGCTGGCCGGGGCCGCTTGGCGGCCCTATCGCGACGCAAGGCCGTTCCCACAAAGTA‑CGTCGTACGCCGATCCTCTGTGGGAGCGGCCTTGCGTCGCGAT‑GGGCTGCAAAGCAGACCCAGAATc > 2:3695312/1‑150 (MQ=255) gcgcCGGCAACTCGCTCGGGGCTGAGCTGGCCGGGGCCGCTTGGCGGCCCTATCGCGACGCAAGGCCGTTCCCACAAAGTA‑CGTCGTACGCCGATCCTCTGTGGGAGCGGCCTTGCGTCGCGAT‑GGGCTGCAAAGCAGACCCatcgcgac < 2:1798293/150‑8 (MQ=255) cgcCGGCAACTCGCTCGGGGCTGAGCTGGCCGGGGCCGCTTGGCGGCCCTATCGCGACGCAAGGCCGTTCCCACAAAGTA‑CGTCGTACGCCGATCCTCTGTGGGAGCGGCCTTGCGTCGCGAT‑GGGCTGCAAAGCAGACCCAGAATCCCa < 2:3011153/150‑1 (MQ=255) cgcCGGCAACTCGCTCGGGGCTGAGCTGGCCGGGGCCGCTTGGCGGCCCTATCGCGACGCAAGGCCGTTCCCACAAAGTA‑CGTCGTACGCCCCTCCACAGTGGGAGCGGCCGTGCGTCGCGAT‑GGGCGGCAAAGCAGAACCCCAATCCCa > 1:3031914/1‑150 (MQ=38) cAACTCGCTCGGGGCTGAGCTGGCCGGGGCCGCTTGGCGGCCCTATCGCGACGCAAGGCCGTTCCCACAAAGTA‑AGTCGTAAGCCGATCCTATGTGGGAGCGGCCTTGCGTCGCGAT‑GGGCTGCAAAGCAGACCCAGAAAACCAACCGCa > 1:4694579/1‑150 (MQ=38) aCTCGCTCGGGGCTGAGCTGGCCGGGGCCGCTTGGCGGCCCTATCGCTACGCAAGGCCGTTCCCACAAAGTA‑CGTCGTACGCCGATCCTCTGTGGGAGCGGCCTTGCGTCGCGAT‑GGGCTGCAAAGCAGACCCAGAATCCCAACCTCAtt < 2:1546730/150‑1 (MQ=255) ctcgctcgGGGCTGAGCTGGCCGGGGCCGCTTGGCGGCCCTATCGCGACGCAAGGCCGTTCCCACAAAGTA‑CGTCGTACGCCGATCCTCTGTGGGAGCGGCCTTGCGTCGCGAT‑GGGCTGCAAAGCAGACCCAGAATCCCAACCTCATTc > 2:2668947/1‑150 (MQ=255) tcgGGGCTGAGCTGGCCGGGGCCGCTTGGCGGCCCTATCGCGACGCAAGGCCGTTCCCACAAAGTA‑CGTCGTACGCCGATCCTCTGTGGGAGCGGCCTTGCGTCGCGAT‑GGGCTGCAAAGCAGACCCAGAATCCCAACCTCATTCCTCaa < 2:5306489/150‑1 (MQ=255) ggggCTGAGCTGGCGGGGGCCGCTTGGCGGCCCTATCGCGACGCAAGGCCGTTCCCACAAAGTA‑CGTCGTACGCCGATCCTCTGTGGGAGCGGCCTTGCGTCGCGAT‑GGGCTGCAAAGCAGACCCAGAATCCCAACCTCATTCCTCAAAc < 2:4561044/150‑1 (MQ=255) acgctgCTGGCCGGGGCCGCTTGGCGGCCCTATCGCGACGCAAGGCCGTTCCCACAAAGTA‑CGTCGTACGCCGATCCTCTGTGGGAGCGGCCTTGCGTCGCGAT‑GGGCTGCAAAGCAGACCCAGAATCCCAACCTCATTCCTCAAACCgg > 2:763262/6‑150 (MQ=255) ctgtgggggCGGGGGCCGTTTGGCGGCCCTTGCGCGACGCAAGGCCGTTCCAAAAAAGTA‑CGTGGTACGCCGATCCTGGGTGGGAGGGGCCTTGCGTCGCGAT‑GGGCTGCAAAGCAGACCCAGAATCCCAACCTCATTCCTCAAACCGGa < 1:2565939/143‑1 (MQ=255) gCTGGCCGGGGCCGCTTGGCGGCCCTATCGCGACGCAAGGCCGTTCCCACAAAGTA‑CGTCGTACGCCGATCCTCTGTGGGAGCGGCCTTGCGTCGCGAT‑GGGCTGCAAAGCAGACCCAGAATCCCAACCTCATTCCTCAAACCGGATCgg < 2:2851402/150‑1 (MQ=255) cgatggggCCGCTTGGCGGCCCTATCGCGACGCAAGGCCGTTCCCCCAAAGTA‑CGTCGTACGCCGCGCCGCAGTGGGAGCGGCCGTGGGTCGCGCT‑GGGCGGCAAAGCAGACCCAGACACCCCAACGCATTCCTCAAAACCGATCGGGcc > 1:4293419/5‑149 (MQ=25) tagggCCGCCAAGCGGCCCTATCGCGACGCAAGGCCGTTCCCACAAAGTA‑CGTCGTACGCCGATCCTCTGTGGGAGCGGCCTTGCGTCGCGAT‑GGGCTGCAAAGCAGACCCCGAATCCCAACCTCAATCCTCCAACCGGATCGGGcacac > 2:4458025/3‑149 (MQ=38) gggCCGCTTGGCGGCCCTATCGCGACGCAAGGCCGTTCCCACAAAGTA‑CGTCGTACGCCGATCCTCTGTGGGAGCGGCCTTGCGTCGCGAT‑GGGCTGCAAAGCAGACCCAGAATCCCAACCTCATTCCTCAAACCGGATCGGGCACAACt < 2:1960700/150‑1 (MQ=255) gCCGCTTGGCGGCCCTATCGCGACGCAAGGCCGTTCCCACAAAGTA‑CGTCGTACGCCGATCCTCTGTGGGAGCGGCCTTGCGTCGCGAT‑GGGCTGCAAAGCAGACCCAGAATCCCAACCTCATTCCTCAAACCGGATCGGGCACAACTcc > 2:1192542/1‑150 (MQ=255) cGCTTGGCGGCCCTATCGCGACGCAAGGCCGTTCCCACAAAGTA‑CGTCGTACGCCGATCCTCTGTGGGAGCGGCCTTGCGTCGCGAT‑GGGCTGCAAAGCAGACCCAGAATCCCAACCTCATTCCTCAAACCGGATCGGGCACAACTCCAc < 1:3893051/150‑1 (MQ=255) gCTTGGCGGCCCTATCGCGACGCAAGGCCGTTCCCACAAAGTA‑CGTCGTACGCCGATCCTCTGTGGGAGCGGCCTTGCGTCGCGAT‑GGGCTGCAAAGCAGACCCAGAATCCCAACCTCATTCCTCAAACCGGATCGGGCACAACTCCAcc < 2:891071/150‑1 (MQ=255) gCTTGGCGGCCCTATCGCGACGCAAGGCCGGTCCCACAAAGTA‑CGTCGTACGCCGCTCCTCTGTGGGAGCGGCCGTGCGTCGCGCT‑GGGCGGCAAAGCAGACCCAGAATACCAACCTCAATCCGCAAACCGGATCGGGCACAACTCCAcc > 1:4847550/1‑150 (MQ=255) cccTATCGCGACGCAAGCCCGTTCCCACAAACTA‑CGACGTACCCCGATCCTCTGTGGGAGCGGCCTTGCGACGCGAT‑GGGCTGCAAAGCAGACCCAGAATCCCAACCTCATTCCTCAAACCGGATCGGGCACAACTCCACCCCTTCCAGc < 1:3092907/150‑1 (MQ=39) cTATCGCGACGCAAGGCCGTTCCCACAAAGTA‑CGTCGTACGCCGATCCTCTGTGGGAGCGGCCTTGCGTCGCGAT‑GGGCTGCAAAGCAGACCCAGAATCCCAACCTCAATCCTCAAACCGGATCGGGCCCAACTCCACCCCTTCCAGCtt > 1:5444990/1‑150 (MQ=255) agcgcgCCGCACGGCCGTGCCCACAATGTA‑CGTCGTCCGCCGATCCTCTGGGGGAGCGGCGTTGCGTCGCGAT‑GGGCTGCAAAGCAGACCCAGAATCCCAACCTCATTCCTCAAACCGGATCGGGCACAACTCCACCCCTTCCAGCTTCt < 1:3679381/148‑1 (MQ=255) tCGCGACGCAAGGCCGTTCCCACAAAGTA‑CGTCGTACGCCGATCCTCTGTGGGAGCGGCCTTGCGTCGCGAT‑GGGCTGCAAAGCAGACCCAGAATCCCAACCTCATTCCTCAAACCGGATCGGGCACAACTCCACCCCATCCAGCTTCTg > 1:4053873/1‑150 (MQ=255) cgAGGCAAGGCCGTACCCACAAAGTA‑CGTCGTACGCCGATCCTCTGTGGGCGCGGCCTTGGGCCGCGAT‑GGGCTGCAAAGCAGACCCAGAATCCCAACCTCATTCCTCAAACCGGATCGGGCACAACTCCACCCCTTCCAGCTTCTGCaa < 1:1576890/150‑1 (MQ=255) catggCCTTTCCCAAAATGTT‑CGTCGTACGCCGATCCTCTGTGGGAGGGGCCTTGCGTCGCGAT‑GGGCTGCAAAGCAGACCCAGAATCCCAACCTCATTCCTCAAACCGGATCGGGCACAACTCCACCCCTTCCAGCTTCTGCAActcct < 1:3367434/147‑1 (MQ=39) cAAGGCCGTTCCCACAAAGTA‑CGTCGTACGCCGATCCTCTGTGGGAGCGGCCTTGCGTCGCGAT‑GGGCTGCAAAGCAGACCCAGAATCCCAACCTCATTCCTCAAACCGGATCGGGCACAACTCCACCCCTTCCAGCTTCTGCAActcct < 2:434548/150‑1 (MQ=255) atggCCGTTCCCACAATGTA‑CGTCGTACGCCTATCCTCTGTGGGAGGGGCCTTGCGTCGCGAT‑GGGCTGCAAAGCAGACCCAGAATCCCAACCTCATTCCTCAAACCGGATCGGGCACAACTCCACCCCTTCCAGCTTCTGCAActcctc < 1:3239258/148‑1 (MQ=255) aaGGCCGTTCCCACAAAGTA‑CGTCGTACGCCGATCCTCTGTGGGAGCGGCCTTGCGTCGCGAT‑GGGCTGCAAAGCAg > 1:3538634/1‑77 (MQ=255) aaGGCCGTTCCCACAAAGTA‑CGTCGTACGCCGATCCTCTGTGGGAGCGGCCTTGCGTCGCGAT‑GGGCTGCAAAGCAg < 2:3538634/77‑1 (MQ=255) aaGGCCGTTCCCACAAAGTA‑CGTCGTACGCCGATCCTCTGTGGGAGCGGCCTTGCGTCGCGAT‑GGGCTGCAAAGCAg < 2:3538685/77‑1 (MQ=255) aaGGCCGCTCCCACAGAGGATCGGCGTACGACG‑TACTTTGTGGGAACGGCCTTGCGTCGCGATAGGGCCGCAAAGCAGACCCAGAATCCCAACCTCATTCCTCAAACCGGATCGGGCACAACTCCACCCCTTCCAGCTTCTGCAActcct > 1:2964405/1‑150 (MQ=17) gCCGTTCCCACAAAGTA‑CGTCGTACGCCGATCCTCTGTGGGAGCGGCCTTGCGTCGCGAT‑GGGCTGCAAAGCAGACCCAGAATCCCAACCTCATTCCTCAAACCGGATCGGGCACAACTCCACCCCTTCCAGCTTCTGCAACTCCTCGAt > 2:4874803/1‑150 (MQ=255) ccGTTCCCACAAAGTA‑CGTCGTACGCCGATCCTCTGTGGGAGCGGCCTTGCGTCGCGAT‑GGGCTGCAAAGCAGACCCAGAATCCCAACCTCATTCCTCAAACCGGATCGGGCACAACTCCACCCCTTCCAGCTTCTGCAACTCCTCGATc > 2:5417887/1‑150 (MQ=255) cGTTCCCACAAAGTA‑CGTCGTACGCCGATCCTCTGTGGGAGCGGCCTTGCGTCGCGAT‑GGGCTGCAAAGCAGACCCAGAATCCCAACCTCATTCCTCAAACCGGATCGGGCACAACTCCACCCCTTCCAGCTTCTGCAACTCCTCGATCa > 2:1695779/1‑150 (MQ=255) gTTCCCACAAAGTA‑CGTCGTACGCCGATCCTCTGTGGGAGCGGCCTTGCGTCGCGAT‑GGGCTGCAAAGCAGACCCAGAATCCCAACCTCATTCCTCAAACCGGATCGGGCACAACTCCACCCCTTCCAGCTTCTGCAACTCCTCGATCAc > 1:2360347/1‑150 (MQ=255) ttCCCACAAAGTA‑CGTCGTACGCCGATCCTCTGTGGGAGCGGCCTTGCGTCGCGAT‑GGGCTGCAAAGCAGACCCAGAATCCCAACCTCATTCCTCAAACCGGATCGGGCACAACTCCACCCCTTCCAGCTTCTGCAACTCCTCGATCAcc > 2:4642641/1‑150 (MQ=255) tCCCACAAAGTA‑CGTCGTACGCCGATCCTCTGTGGGAGCGGCCTTGCGTCGCGAT‑GGGCTGCAAAGCAGACCCAGAATCCCAACCTCATTCCTCAAACCGGATCGGGCACAACTCCACCCCTTCCAGCTTCTGCAACTCCTCGATCACCt > 1:581788/1‑150 (MQ=255) cccACAAAGTA‑CGTCGTACGCCGATCCTCTGTGGGAGCGGCCTTGCGTCGCGAT‑GGGCTGCAAAGCAGACCCAGAATCCCAACCTCATTCCTCAAACCGGATCGGGCACAACTCCACCCCTTCCAGCTTCTGCAACTCCTCGATCACCTg > 2:2483619/1‑150 (MQ=255) cacaAAGTA‑CGTCGTACGCCGATCCTCTGTGGGAGCGGCCTTGCGTCGCGAT‑GGGCTGCAAAGCAGACCCAGAATCCCAACCTCATTCCTCAAACCGGATCGGGCACAACTCCACCCCTTCCAGCTTCTGCAACTCCTCGATCACCTGCg > 2:1931904/1‑150 (MQ=255) cacaAAGTA‑CGTCGTACGCCGATCCTCTGTGGGAGCGGCCTTGCGTCGCGAT‑GGGCTGCAAAGCAGACCCAGAATCCCAACCTCATTCCTCAAACCGGATCGGGCACAACTCCACCCCTTCCAGCTTCTGCAACTCCTCGATCACCTGCg > 2:1538699/1‑150 (MQ=255) cacaAAGTA‑CGTCGTACGCCGATCCTCTGTGGGAGCGGCCTTGCGTCGCGAT‑GGGCTGCAAAGCAGACCCAGAATCCCAACCTCATTCCTCAAACCGGATCGGGCACAACTCCACCCCTTCCAGCTTCTGCAACTCCTCGATCACCTGCg > 1:4737806/1‑150 (MQ=255) tcggcgTA‑CGACGTACGCCGATCCTCTGTGGGAGCGGCCTTGCGTCGCGAT‑GGGCTGCAAAGCAGACCCAGAATCCCAACCTCATTCCTCAAACCGGATCGGGCACAACTCCACCCCTTCCAGCTTCTGCAACTCCTCGATCACCTGCgg > 1:3191064/6‑150 (MQ=255) caAAGTA‑CGTCGTACGCCGATCCTCTGTGGGAGCGGCCTTGCGTCGCGAT‑GGGCTGCAAAGCAGACCCAGAATCCCAACCTCATTCCTCAAACCGGATCGGGCACAACTCCACCCCTTCCAGCTTCTGCAACTCCTCGATCACCTGCGGc > 1:224186/1‑150 (MQ=255) caAAGTA‑CGTCGTACGCCGATCCTCTGTGGGAGCGGCCTTGCGTCGCGAT‑GGGCTGCAAAGCAGACCCAGAATCCCAACCTCATTCCTCAAACCGGATCGGGCACAACTCCACCCCTTCCAGCTTCTGCAACTCCTCGATCACCTGCGGc > 1:4576024/1‑150 (MQ=255) gTA‑CTTCGTACGCCGAGCCTCGGTGGGAGCGGCCTTGCGTCGCGAT‑GGGCTGCAAAGCAGACCCAGAATCCCAACCTCATTCCTCAAACCGGATCGGGCACAACTCCACCCCTTCCAGCTTCTGCAACTCCTCGATCACCTGCGGCCTgg < 1:5220316/150‑1 (MQ=255) gtcgtACGCCGATCCTCTGTGGGAGCGGCCTTGCGTCGCGAT‑GGGCTGCAAAGCAGACCCAGAATCCCAACCTCATTCCTCAAACCGGATCGGGCACAACTCCAccc > 2:1493988/1‑107 (MQ=255) gtcgtACGCCGATCCTCTGTGGGAGCGGCCTTGCGTCGCGAT‑GGGCTGCAAAGCAGACCCAGAATCCCAACCTCATTCCTCAAACCGGATCGGGCACAACTCCAccc < 1:1493988/107‑1 (MQ=255) tcgtACGCCGATCCTCTGTGGGAGCGGCCTTGCGTCGCGAT‑GGGCTGCAAAGCAGACCCAGAATCCCAACCTCATTCCTCAAACCGGATCGGGCACAACTCCACCCCTTCCAGCTTCTGCAACTCCTCGATCACCTGCGGCCTGGCCCgg > 2:5140152/1‑150 (MQ=255) cgtACGCCGATCCTCTGTGGGAGCGGCCTTGCGTCGCGAT‑GGGCTGCAAAGCAGACCCAGAATCCCAACCTCATTCCTCAAACCGGATCGGGCACAACTCCACCCCTTCCAGCTTCTGCAACTCCTCGATCACCTGCGGCCTGGCCCGGt < 2:224186/150‑1 (MQ=255) tACGCCGATCCTCTGTGGGAGCGGCCTTGCGTCGCGAT‑GGGCTGCAAAGCAGACCCAGAATCCCAACCTCATTCCTCAAACCGGATCGGGCACAACTCCACCCCTTCCAg > 1:1854903/1‑110 (MQ=255) gCCGATCCTCTGTGGGAGCGGCCTTGCGTCGCGAT‑GGGCTGCAAAGCAGACCCAGAATCCCAACCTCATTCCTCAAACCGGATCGGGCACAACTCCACCCCTTCCAGCTTCTGCAACTCCTCGATCACCTGCGGCCTGGCCCGGTGCAAg > 2:4457340/1‑150 (MQ=255) cGATCCTGTGTGGGTGCGGCCTTGCGTCGCGAT‑GGGCTGCAAAGCAGACCCAGAATCCCAACCTCATTCCTCAAACCGGATCGGGCACAACTCCACCCCTTCCAGCTTCTGCAACTCCTCGATCACCTGCGGCCTGGCCCGGTGCAAGGt < 1:5244790/150‑1 (MQ=255) cGATCCTCTGTGGGAGCGGCCTTGCGTCGCGAT‑GGGCTGCAAAGCAGACCCAGAATCCCAACCTCATTCCTCAAACCGGATCGGGCACAACTCCACCCCTTCCAGCTTCTGCAACTCCTCGATCACCTGCGGCCTGGCCCGGTGCAAGGt > 1:3321452/1‑150 (MQ=255) aTCCTCTGTGGGAGCGGCCTTGCGTCGCGAT‑GGGCTGCAAAGCAGACCCAGAATCCCAACCTCATTCCTCAAACCGGATCGGGCACAACTCCACCCCTTCCAGCt > 1:4366690/1‑105 (MQ=255) | GCCTTCTTTATCTGGCGTGGCGTGAGGCCGGATACGGCGCCGGTGTTGGCTGAACTGCGCCGGCAACTCGCTCGGGGCTGAGCTGGCCGGGGCCGCTTGGCGGCCCTATCGCGACGCAAGGCCGTTCCCACAAAGTA‑CGTCGTACGCCGATCCTCTGTGGGAGCGGCCTTGCGTCGCGAT‑GGGCTGCAAAGCAGACCCAGAATCCCAACCTCATTCCTCAAACCGGATCGGGCACAACTCCACCCCTTCCAGCTTCTGCAACTCCTCGATCACCTGCGGCCTGGCCCGGTGCAAGGT > NC_002947/84942‑85238 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |