BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	474,202:1	+G	5.0%	coding (786/1983 nt)	dnaG ←	DNA primase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	474,202	1	.	G	5.0%	896.6 / 36.5	200	coding (786/1983 nt)	dnaG	DNA primase
Reads supporting (aligned to +/- strand): ref base . (101/89); new base G (2/8); total (103/97)
Fisher's exact test for biased strand distribution p-value = 5.28e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 212 total aligned reads displayed.

CCGTCAAAACAGAACAGCACGCTGGGCACCACGCGGAACAGGCGCTTGAGGTGCTCTTCGCTGGTGGCGGTGCCAAGGGTGGCCACGGCATTGCGCAGGCCTTGCTGGGCCAGGGCAATGACGTCCATGTAGCCCTCGACGACGATGATC‑TCGTCGAGGTTGCGGTTGTGCTTGCGCGCCTCGTACAGCCCGTACAGCTCCTGCCCCTTGTGGAAAACCGGGGTTTCCGGGGAGTTCAGGTACTTGGGCTTGTCGTCGCCGAGCACCCGGCCACCGAAGGCGATGATGCGCCCGCGGCT  >  NC_002947/474053‑474351

ccGTCAAAACAGACCAGCACGCTGGGCACCACGCGGAACAGGCGCTTGAGGTGCTCTTCGCTGGTGGCGGTGCCAAGGGTGGCCACGGCATTGCGCAGGCCTTGCTGGGCCAGGGCAATGACGTCCATGTAGCCCTCGACGACGATGAtc                                                                                                                                                        <  1:289318/150‑1 (MQ=255)

ccGTCAAAACAGAACAGCACGCTGGGCACCACGCGGAACAGGCGCTTGAGGTGCTCTTCGCTGGTGGCGGTGCCAAGGGTGGCCACGGCATTGCGCAGGCCTTGCTGGGCCAGGGCAATGACGTCCATGTAGCCCTCGACGACGATGAtc                                                                                                                                                        <  2:1705069/150‑1 (MQ=255)

  gTCAAAACAGAACAGCACGCTGGGCACCACGCGGAACAGGCGCTTGAGGTGCTCTTCGCTGGTGGCGGTGCCAAGGGTGGCCACGGCATTGCGCAGGCCTTGCTGGGCCAGGGCAATGACGTCCATGTAGCCCTCGACGACGATGAtc‑tc                                                                                                                                                     <  2:4323012/150‑1 (MQ=255)

     aaaaCAGAACAGCACGCTGGGCACCACGCGGAACAGGCGCTTGAGGTGCTCTTCGCTGGTGGCGGTGCCAAGGGTGGCCACGGCATTGCGCAGGCCTTGCTGGGCCAGGGCAATGACGTCCATGTAGCCCTCGACGACGATGATC‑tcgtc                                                                                                                                                  <  2:1872942/150‑1 (MQ=255)

      aaaCAGAACAGCACGCTGGGCACCACGCGGAACAGGCGCTTGAGGTGCTCTTCGCTGGTGGCGGTGCCAAGGGTGGCCACGGCATTGCGCAGGCCTTGCTGGGCCAGGGCAATGACGTCCATGTAGCCCTCGACGACGATGATC‑tcgtcg                                                                                                                                                 >  1:5067700/1‑150 (MQ=255)

       aacagaacagCACGCTGGGCACCACGCGGAACAGGCGCTTGAGGTGCTCTTCGCTGGTGGCGGTGCCAAGGGTGGCCACGGCATTGCGCAGGCCTTGCTGGGCCAGGGCAATGACGTCCATGTAGCCCTCGACGA‑GATCATCGTCGTCGa                                                                                                                                                <  2:1121021/150‑1 (MQ=255)

       aacagaacagCACGCTGGGCACCACGCGGAACAGGCGCGTGAGGTGCTCTTCGCTGGTGGCGGTGCCAAGGGTGGCCACGGCATTGCGCAGGCCTTGCTGGGCCAGGGCAATGACGTCCATGTAGCCCTCGACGACGATGATC‑TCGTCGa                                                                                                                                                >  2:3520203/1‑150 (MQ=255)

       aacagaacacCACGCTGGGCACCACGCGCAACAGGCGCTTGAGGTGCTCTTCGCTGGTGGCGGTGCCAAGGCTGCCCACGGCATTGCGCAGGCCTTGCTGGGCCAGGGCAATGACGTCCATGTAGCCCTCGACGACGATGATC‑TCGTCGa                                                                                                                                                <  2:183463/150‑1 (MQ=255)

        acagaacagCACGCTGGGCACCACGCGGAACAGGCGCTTGAGGTGCTCTTCGCTGGTGGCGGTGCCAAGGGTGGCCACGGCATTGCGCAGGCCTTGCTGGGCCAGGGCAATGACGTCCATGTAGCCCTCGACGA‑GATCATCGTCGTCGAg                                                                                                                                               <  2:836745/150‑1 (MQ=255)

          agaacagCACGCTGGGCACCACGCGGAACAGGCGCTTGAGGTGCTCTTCGCTGGTGGCGGTGCCAAGGGTGGCCACGGCATTGCGCAGGCCTTGCTGGGCCAGGGCAATGACGTCCATGTAGCCCTCGACGACGATGATC‑TCGTCGAGGt                                                                                                                                             >  1:4240910/1‑150 (MQ=255)

              cagCACGCTGGGCACCACGCGGAACAGGCGCTTGAGGTGCTCTTCGCTGGTGGCGGTGCCAAGGGTGGCCACGGCATTGCGCAGGCCTTGCTGGGCCAGGGCAATGACGTCCATGTAGCCCTCGACGACGATGATC‑TCGTCGAGGTTGCg                                                                                                                                         >  1:4411949/1‑150 (MQ=255)

               agCACGCTGGGCACCACGCGGAACAGGCGCTTGAGGTGCTCTTCGCTGGTGGCGGTGCCAAGGGTGGCCACGGCATTGCGCAGGCCTTGCTGGGCCAGGGCAATGACGTCCATGTAGCCCTCGACGACGATGATC‑TCGTCGAGGTTGCgg                                                                                                                                        <  1:1495212/150‑1 (MQ=255)

                gCACGCTGGGCACCACGCGGAACAGGCGCTTGAGGTGCTCTTCGCTGGTGGCGGTGCCAAGGGTGGCCACGGCATTGCGCAGGCCTTGCTGGGCCAGGGCAATGACGTCCATGTAGCCCTCGACGACGATGATC‑TCGTCGAGGTTGCGGt                                                                                                                                       >  1:322178/1‑150 (MQ=255)

                gCACGCTGGGCACCACGCGGAACAGGCGCTTGAGGTGCTCTTCGCTGGTGGCGGTGCCAAGGGTGGCCACGGCATTGCGCAGGCCTTGCTGGGCCAGGGCAATGACGTCCATGTAGACCTCGACGA‑GATCATCGTCGTCGAgggctacat                                                                                                                                       <  1:829178/150‑8 (MQ=255)

                 cACGCTGGGCACCACGCGGAACAGGCGCTTGAGGTGCTCTTCGCTGGTGGCGGTGCCAAGGGTGGCCACGGCATTGCGCAGGCCTTGCTGGGCCAGGGCAATGACGTCCATGTAGCCCTCGACGACGATGATC‑TCGTCGAGGTTGCGGtg                                                                                                                                      >  2:4884159/1‑149 (MQ=255)

                       gggCACCACGCGGAACAGGCGCTTGAGGTGCTCTTCGCTGGTGGCGGTGCCAAGGGTGGCCACGGCATTGCGCAGGCCTTGCTGGGCCAGGGCAATGACGTCCATGTAGCCCTCGACGACGATGATC‑TCGTCGAGGTTGCGGTTGTGCtt                                                                                                                                <  2:750461/150‑1 (MQ=255)

                        ggCACCACGCGGAACAGGCGCTTGAGGTGCTCTTCGCTGGTGGCGGTGCCAAGGGTGGCCACGGCATTGCGCAGGCCTAGCTGGGCCAGGGCAATGACGTCCATGTAGCCCTCGACGACGATGATC‑TCGTCGAGGTTGCGGTTGTGCTTg                                                                                                                               <  2:385273/150‑1 (MQ=255)

                             cacGCGGAACAGGCGCTTGAGGTGCTCTTCGCTGGTGGCGGTGCCAAGGGTGGCCACGGCATTGCGCAGGCCTTGCTGGGCCAGGGCAATGACGTCCATGTAGCCCTCGACGACGATGATC‑TCGTCGAGGTTGCGGTTGTGCTTgcgcgc                                                                                                                          >  1:2471214/1‑150 (MQ=255)

                             cacGCGGAACAGGCGCTTGAGGTGCTCTTCGCTGGTGGCGGTGCCAAGGGTGGCCACGGCATTGCGCAGGCCTTGCTGGGCCAGGGCAATGACGTCCATGTAGCCCTCGACGACGATGATC‑TCGTCGAGGTTGCGGTTGTGCTTgcgcgc                                                                                                                          <  1:2790573/150‑1 (MQ=255)

                              acGCGGAACAGGCGCTTGAGGTGCTCTTCGCTGGTGGCGGTGCCAAGGGTGGCCACGGCATTGCGCAGGCCTTGCTGGGCCAGGGCAATGACGTCCATGTAGCCCTCGACGACGATGATC‑TCGTCGAGGTTGCGGTTGTGCTTGCGCGcc                                                                                                                         <  1:1199048/150‑1 (MQ=255)

                              acGCGGAACAGGCGCTTGAGGTGCTCTTCGCTGGTGGCGGTGCCAAGGGTGGCCACGGCATTGCGCAGGCCTTGCTGGGCCAGGGCAATGACGTCCATGTAGCCCTCGACGACGATGATC‑TCGTCGAGGTTGCGGTTGTGCTTGCGCGcc                                                                                                                         >  1:2546915/1‑150 (MQ=255)

                               cgcgGAACAGGCGCTTGAGGTGCTCTTCGCTGGTGGCGGTGCCAAGGGTGGCCACGGCATTGCGCAGGCCTTGCTGGGCCAGGGCAATGACGTCCATGTAGCCCTCGACGACGATGATC‑TCGTCGAGGTTGCGGTTGTGCTTGCGCGCCt                                                                                                                        <  2:2471214/150‑1 (MQ=255)

                                gcgGAACAGGCGCTTGAGGTGCTCTTCGCTGGTGGCGGTGCCAAGGGTGGCCACGGCATTGCGCAGGCCTTGCTGGGCCAGGGCAATGACGTCCATGTAGCCCTCGACGACGATGATC‑TCGTCGAGGTTGCGGTTGTGCTTGCGCGCCTc                                                                                                                       <  1:5357352/150‑1 (MQ=255)

                                      cAGGCGCTTGAGGTGCTCTTCGCTGGTGGCGGTGCCAAGGGTGGCCACGGCATTGCGCAGGCCTTGCTGGGCCAGGGCAATGACGTCCATGTAGCCCTCGACGACGATGATC‑TCGTCGAGGTTGCGGTTGTGCTTGCGCGCCTCGTACAg                                                                                                                 <  2:2965538/150‑1 (MQ=255)

                                         gcgcTTGAGGTGCTCTTCGCTGGTGGCGGTGCCAAGGGTGGCCACGGCATTGCGCAGGCCTTGCTGGGCCAGGGCAATGACGTCCATGTAGCCCTCGACGACGATGATC‑TCGTCGAGGTTGCGGTTGTGCTTGCGCGCCTCGTACAGccc                                                                                                              >  1:1807750/1‑150 (MQ=255)

                                         gcgcTTGAGGTGCTCTTCGCTGGTGGCGGTGCCAAGGGTGGCCACGGCATTGCGCAGGCCTTGCTGGGCCAGGGCAATGACGTCCATGTAGCCCTCGACGACGATGATC‑TCGTCGAGGTTGCGGTTGTGCTTGCGCGCCTCGTACAGccc                                                                                                              >  1:4056541/1‑150 (MQ=255)

                                            cTTGAGGTGCTCTTCGCTGGTGGCGGTGCCAAGGGTGGCCACGGCATTGCGCAGGCCTTGCTGGGCCAGGGCAATGACGTCCATGTAGCCCTCGACGACGATGATC‑TCGTCGAGGTTGCGGTTGTGCTTGCGCGCCTCGTACAGCCCGTa                                                                                                           <  1:4959077/150‑1 (MQ=255)

                                               gAGGTGCTCTTCGCTGGTGGCGGTGCCAAGGGTGGCCACGGCATTGCGCAGGCCTTGCTGGGCCAGGGCAATGACGTCCATGTAGCCCTCGACGACGATGATC‑TCGTCGAGGTTGCGGTTGTGCTTGCGCGCCTCGTACAGCCCGTACAg                                                                                                        <  1:3879477/150‑1 (MQ=255)

                                                    gCTCTTCGCTGGTGGCGGTGCCAAGGGTGGCCACGGCATTGCGCAGGCCTTGCTGGGCCAGGGCAATGACGTCCATGTAGCCCTCGACGACGATGATC‑TCGTCGAGGTTGCGGTTGTGCTTGCGCGCCTCGTACAGCCCGTACAGCTCCt                                                                                                   >  1:4834928/1‑150 (MQ=255)

                                                      tctTCGCTGGTGGCGGTGCCAAGGGTGGCCACGGCATTGCGCAGGCCTTGCTGGGCCAGGGCAATGACGTCCATGTAGCCCTCGACGACGATGATC‑TCGTCGAGGTTGCGGTTGTGCTTGCGCGCCTCGTACAGCCCGTACAGCTCCTGc                                                                                                 >  1:1373870/1‑150 (MQ=255)

                                                        ttCGCTGGTGGCGGTGCCAAGGGTGGCCACGGCATTGCGCAGGCCTTGCTGGGCCAGGGCAATGACGTCCATGTAGCCCTCGACGACGATGATC‑TCGTCGAGGTTGCGGTTGTGCTTGCGCGCCTCGTACAGCCCGTACAGCTCCTGccc                                                                                               <  2:4838000/150‑1 (MQ=255)

                                                        ttCGCTGGTGGCGGTGCCAAGGGTGGCCACGGCATTGCGCAGGCCTTGCTGGGCCAGGGCAATGACGTCCATGTAGCCCTCGACGACGATGATC‑TCGTCGAGGTTGCGGTTGTGCTTGCGCGCCTCGTACAGCCCGTACAGCTCCTGccc                                                                                               >  1:4978327/1‑150 (MQ=255)

                                                          cGCTGGTGGCGGTGCCAAGGGTGGCCACGGCATTGCGCAGGCCTTGCTGGGCCAGGGCAATGACGTCCATGTAGCCCTCGACGACGATGATC‑TCGTCGAGGTTGCGGTTGTGCTTGCGCGCCTCGTACAGCCCGTACAGCTCCTGCCCCt                                                                                             <  2:1473735/150‑1 (MQ=255)

                                                             tggtggCGGTGCCAAGGGTGGCCACGGCATTGCGCAGGCCTTGCTGGGGCAGGGCAATGAGGTCCATGTAGCCCTCGACGACGATGATC‑TCGTCGAGGTTGCGGTTGTGCTTGCGCGCGTCGTACAGCCCGTACAGCTCCTGCCCCTtgt                                                                                          >  2:5159410/1‑150 (MQ=255)

                                                             tggtggCGGTGCCAAGGGTGGCCACGGCATTGCGCAGGCCTTGCTGGGCCAGGGCAATGACGTCCATGTAGCCCTCGACGACGATGATC‑TCGTCGAGGTTGCGGTTGTGCTTGCGCGCCTCGTACAGCCCGTACAGCTCCTGCCCCTtgt                                                                                          >  2:264916/1‑150 (MQ=255)

                                                             tggtggCGGTGCCAAGGGTGGCCACGGCATTGCGCAGGCCTTGCTGGGCCAGGGCAATGACGTCCATGTAGCCCTCGACGACGATGATC‑TCGTCGAGGTTGCGGTTGTGCTTGCGCGCCTCGTACAGCCCGTACAGCTCCTGCCCCTtgt                                                                                          >  2:2590119/1‑150 (MQ=255)

                                                             tggtggCGGTGCCAAGGGTGGCCACGGCATTGCGCAGGCCTTGCTGGGCCAGGGCAATGACGTCCATGTAGCCCTCGACGACGATGATC‑TCGTCGAGGTTGCGGTTGTGCTTGCGCGCCTCGTACAGCCCGTACAGCTCCTGCCCCTtgt                                                                                          <  1:4766993/150‑1 (MQ=255)

                                                             tggtggCGGTGCCAAGGGTGGCCACGGCATTGCGCAGGCCTTGCTGGGCCAGGGCAATGACGTCCATGTAGCCCTCGACGACGATGATC‑TCGTCGAGGTTGCGGTTGTGCTTGCGCGCCTCGTACAGCCCGTACAGCTCCTGCCCCTtgt                                                                                          >  1:4770553/1‑150 (MQ=255)

                                                                  gCGGTGCCAAGGGTGGCCACGGCATTGCGCAGGCCTTGCTGGGCCAGGGCAATGACGTCCATGTAGCCCTCGACGACGATGATC‑TCGTCGAGGTTGCGGTTGTGCTTGCGCGCCTCGTACAGCCCGTACAGCTCCTGCCCCTTGTGGaaa                                                                                     >  2:2889925/1‑150 (MQ=255)

                                                                   cGGTGCCAAGGGTGGCCACGGCATTGCGCAGGCCTTGCTGGGCCAGGGCAATGACGTCCATGTAGCCCTCGACGACGATGATC‑TCGTCGAGGTTGCGGTTGTGCTTGCGCGCCTCGTACAGCCCGTACAGCTCCTGCCCCTTGTGGaaaa                                                                                    >  1:3515383/1‑150 (MQ=255)

                                                                      tGCCAAGGGTGGCCACGGCATTGCGCAGGCCTTGCTGGGCCAGGGCAATGACGTCCATGTAGCCCTCGACGACGATGATC‑TCGTCGAGGTTGCGGTTGTGCTTGCGCGCCTCGTACAGCCCGTACAGCTCCTGCCCCTTGTGGAAAACCg                                                                                 <  2:2913730/150‑1 (MQ=255)

                                                                       gCCAAGGGTGGCCACGGCATTGCGCAGGCCTTGCTGGGCCAGGGCAATGACGTCCATGTAGCCCTCGACGACGATGATC‑TCGTCGAGGTTGCGGTTGTGCTTGCGCGCCTCGTACAGCCCGTACAGCTCCTGCCCCTTGTGGAAAACCgg                                                                                <  2:1688216/150‑1 (MQ=255)

                                                                       gCCAAGGGTGGCCACGGCATTGCGCAGGCCTTACTGGGCCAGGGCAATGACGTCCATGTAGCCCTCGACGACGATGATC‑TCGTCGAGGTTGCGGTTGTGCTTGCGCGCCTCGTACAGCCCGTACAGCTCCTGCCCCTTGTGGAAAACCgg                                                                                <  2:2901456/150‑1 (MQ=255)

                                                                        ccAAGGGTGGCCACGGCATTGCGCAGGCCTTGCTGGGCCAGGGCAATGACGTCCATGTAGCCCTCGACGACGATGATC‑TCGTCGAGGTTGCGGTTGTGCTTGCGCGCCTCGTACAGCCCGTACAGCTCCTGCCCCTTGTGGAAAACCggg                                                                               <  2:4489192/150‑1 (MQ=255)

                                                                        ccAAGGGTGGCCACGGCATTGCGCAGGCCTTGCTGGGCCAGGGCAATGACGTCCATGTAGCCCTCGACGACGATGATC‑TCGTCGAGGTTGCGGTTGTGCTTGCGCGCCTCGTACAGCCCGTACAGCTCCTGCCCCTTGTGGAAAACCggg                                                                               <  1:2680571/150‑1 (MQ=255)

                                                                        ccAAGGGTGGCCACGGCATTGCGCAGGCCTTGCTGGGCCAGGGCAATGACGTCCATGTAGCCCTCGACGACGATGATC‑TCGTCGAGGTTGCGGTTGTGCTTGCGCGCCTCGTACAGCCCGTACAGCTCCTGCCCCTTGTGGAAAACCggg                                                                               <  1:4175611/150‑1 (MQ=255)

                                                                        ccAAGGGTGGCCACGGCATTGCGCAGGCCTTGCTGGGCCAGGGCAATGACGTCCATGTAGCCCTCGACGACGATGATC‑TCGTCGAGGTTGCGGTTGTGCTTGCGCGCCTCGTACAGCCCGTACAGCTCCTGCCCCTTGTGGAAAACCggg                                                                               <  2:3219314/150‑1 (MQ=255)

                                                                        ccAAGGGTGGCCACGGCATTGCGCAGGCCTTGCTGGGCCAGGGCAATGACGTCCATGTAGCCCTCGACGACGATGATC‑TCGTCGAGGTTGCGGTTGTGCTTGCGCGCCTCGTACAGCCCGTACAGCTCCTGCCCCTTGTGGAAAACCggg                                                                               <  1:533171/150‑1 (MQ=255)

                                                                        ccAAGGGTGGCCACGGCATTGCGCAGGCCTTGCTGGGCCAGGGCAATGACGTCCATGTAGCCCTCGACGACGATGATC‑TCGTCGAGGTTGCGGTTGTGCTTGCGCGCCTCGTACAGCCCGTACAGCTCCTGCCCCTTGTGGAAAACCggg                                                                               <  1:3063867/150‑1 (MQ=255)

                                                                          aaGGGTGGCCACGGCATTGCGCAGGCCTTGCTGGGCCAGGGCAATGACGTCCATGTAGCCCTCGACGACGATGATC‑TCGTCGAGGTTGCGGTTGTGCTTGCGCGCCTCGTACAGCCCGTACAGCTCCTGCCCCTTGTGGAAAACCGGGGt                                                                             >  2:832652/1‑150 (MQ=255)

                                                                          aaGGGTGGCCACGGCATAGCGCAGGCCTTGCTGGGCCAGGGCAATGACGTCCATGTAGCCCTCGACGACGATGATC‑TCGTCGAGGTTGCGGTTGTGCTTGCGCGCCTCGTACAGCCCGTACAGCTCCTGCCCCTTGTGGAAAACCGGGGt                                                                             >  2:522614/1‑150 (MQ=255)

                                                                             ggTGGCCACGGCATTTCGCAGGCCTTGCTGGGCCAGGGCAATGACGTCCATGTAGCCCTCGACGACGATGATC‑TCGTCGAGGTTGCGGTTGTGCGTGCGCGCCTCGTACATCCCGTACAGCTCCTGCCCCTTGTGGAAAACCGGGGTTTc                                                                          >  2:1108777/1‑150 (MQ=255)

                                                                               tGGCCACGGCATTGCGCAGGCCTTGCTGGGCCAGGGCAATGACGTCCATGTAGCCCTCGACGACGATGATC‑TCGTCGAGGTTGCGGTTGTGCTTGCGCGCCTCGTACAGCCCGTACAGCTCCTGCCCCTTGTGGAAAACCGGGGTTTCCg                                                                        >  2:4550861/1‑150 (MQ=255)

                                                                                 gCCACGGCATTGCGCAGGCCTTGCTGGGCCAGGGCAATGACGTCCATGTAGCCCTCGACGACGATGATC‑TCGTCGAGGTTGCGGTTGTGCTTGCGCGCCTCGTACAGCCCGTACAGCTCCTGCCCCTTGTGGAAAACCGGGGTTTCCggg                                                                      <  2:358373/150‑1 (MQ=255)

                                                                                 gCCACGGCATTGCGCAGGCCTTGCTGGGCCAGGGCAATGACGTCCATGTAGCCCTCGACGACGATGATC‑TCGTCGAGGTTGCGGTTGTGCTTGCGCGCCTCGTACAGCCCGTACAGCTCCTGCCCCTTGTGGAAAACCGGGGTTTCCggg                                                                      <  2:4673247/150‑1 (MQ=255)

                                                                                  ccACGGCATTGCGCAGGCCTTGCTGGGCCAGGGCAATGACGTCCATGTAGCCCTCGACGACGATGATC‑TCGTCGAGGTTGCGGTTGTGCTTGCGCGCCTCGTACAGCCCGTACAGCTCCTGCCCCTTGTGGAAAACCGGGGTTTCCgggg                                                                     >  2:405588/1‑150 (MQ=255)

                                                                                  ccACGGCATTGCGCAGGCCTTGCTGGGCCAGGGCAATGACGTCCATGTAGCCCTCGACGACGATGATC‑TCGTCGAGGTTGCGGTTGTGCTTGCGCGCCTCGTACAGCCCGTACAGCTCCTGCCCCTTGTGGAAAACCGGGGTTTCCgggg                                                                     >  2:2180449/1‑150 (MQ=255)

                                                                                   cACGGCATTGCGCAGGCCTTGCTGGGCCAGGGCAATGACGTCCATGTAGCCCTCGACGACGATGATC‑TCGTCGAGGTTGCGGTTGTGCTTGCGCGCCTCGTACAGCCCGTACAGCTCCTGCCCCTTGTGGAAAACCGGGGTTTCCGGGGa                                                                    <  1:3184644/150‑1 (MQ=255)

                                                                                   cACGGCATTGCGCAGGCCTTGCTGGGCCAGGGCAATGACGTCCATGTAGCCCTCGACGACGATGATC‑TCGTCGAGGTTGCGGTTGTGCTTGCGCGCCTCGTACAGCCCGTACAGCTCCTGCCCCTTGTGGAAAACCGGGGTTTCCGGGGa                                                                    <  1:2300367/150‑1 (MQ=255)

                                                                                    aCGGCATTGCGCAGGCCTTGCTGGGCCAGGGCAATGACGTCCATGTAGCCCTCGACGACGATGATC‑TCGTCGAGGTTGCGGTTGTGCTTGCGCGCCTCGTACAGCCCGTACAGCTCCTGCCCCTTGTGGAAAACCGGGGTTTCCg                                                                        >  1:3548362/1‑145 (MQ=255)

                                                                                    aCGGCATTGCGCAGGCCTTGCTGGGCCAGGGCAATGACGTCCATGTAGCCCTCGACGACGATGATC‑TCGTCGAGGTTGCGGTTGTGCTTGCGCGCCTCGTACAGCCCGTACAGCTCCTGCCCCTTGTGGAAAACCGGGGTTTCCGGGGAg                                                                   >  1:1901956/1‑150 (MQ=255)

                                                                                      ggCATTGCGCAGGCCTTGCTGGGCCAGGGCAATGACGTCCATGTAGCCCTCGACGACGATGATC‑TCGTCGAGGTTGCGGTTGTGCTTGCGCGCCTCGTACAGCCCGTACAGCTCCTGCCCCTTGTGGAAAACCGGGGTTTCCGGGGAGtt                                                                 >  1:1576903/1‑150 (MQ=255)

                                                                                       gCATTGCGCAGGCCTTGCTGGGCCAGGGCAATGACGTCCATGTAGCCCTCGACGACGATGATC‑TCGTCGAGGTTGCGGTTGTGCTTGCGCGCCTCGTACAGCCCGTACAGCTCCTGcccc                                                                                              >  2:2752474/1‑120 (MQ=255)

                                                                                       gCATTGCGCAGGCCTTGCTGGGCCAGGGCAATGACGTCCATGTAGCCCTCGACGACGATGATC‑TCGTCGAGGTTGCGGTTGTGCTTGCGCGCCTCGTACAGCCCGTACAGCTCCTGCCCCTTGTGGAAAACCGGGGTTTCCGGGGAGTTc                                                                <  2:3039296/150‑1 (MQ=255)

                                                                                        cATTGCGCAGGCCTTGCTGGGTCAGGGCAATGACGTCCATGTAGCCCTCGACGCCGATGATC‑TCGTCGAGGTTGCGGTTGTGCTTGCGCGCCTCGTACAGCCCGTACAGCTCCTGCCCCTTGTGGAAAACCGGGGTTTCCGGGGAGTTCa                                                               <  2:1740853/150‑1 (MQ=255)

                                                                                            gcgcAGGCCTTGCTGGGCCAGGGCAATGACGTCCATGTAGCCCTCGACGACGATGATC‑TCGTCGAGGTTGCGGTTGTGCTTGCGCGCCTCGTACAGCCCGTACAGCTCCTGCCCCTTGTGGAAAACCGGGGTTTCCGGGGAGTTCAGGtc                                                           >  2:340646/1‑149 (MQ=255)

                                                                                              gcAGGCCTTGCTGGGCCAGGGCAATGACGTCCATGTAGCCCTCGACGACGATGATC‑TCGTCGAGGTTGCGGTTGTGCTTGCGCGCCTCGTACAGCCCGTACAGCTCCTGCCCCTTGTGGAAAACCGGGGTTTCCGGGGAGTTCAGGTACt                                                         >  2:149572/1‑150 (MQ=255)

                                                                                               cAGGCCTTGCTGGGCCAGGGCAATGACGTCCATGTAGCCCTCGACGACGATGATC‑TCGTCGAGGTTGCGGTTGTGCTTGCGCGCCTCGTACAGCCCGTACAGCTCCTGCCCCTTGTGGAAAACCGGGGTTTCCGGGGAGTTCAGGTACtt                                                        <  2:1000064/150‑1 (MQ=255)

                                                                                                  gCCTTGCTGGGCCAGGGCAATGACGTCCATGTAGCCCTCGACGACGATGATC‑TCGTCGAGGTTGCGGTTGTGCTTGCGCGCCTCGTACAGCCCGTACAGCTCCTGCCCCTTGTGGAAAACCGGGGTTTCCGGGGAGTTCAGGTACTTggg                                                     >  2:1303247/1‑150 (MQ=255)

                                                                                                         tGGGCCAGGGCAATGACGTCCATGTAGCCCTCGACGACGATGATC‑TCGTCGAGGTTGCGGTTGTGCTTGCGCGCCTCGTACAGCCCGTACAGCTCCTGCCCCTTGTGGAAAACCGGGGTTTCCGGGGAGTTCAGGTACTTGGGCTTgtcg                                              <  2:4441898/150‑1 (MQ=255)

                                                                                                          gggCCAGGGCAATGACGTCCATGTAGCCCTCGACGACGATGATC‑TCGTCGAGGTTGCGGTTGTGCTTGCGCGCCTCGTACAGCCCGTACAGCTCCTGCCCCTTGTGGAAAACCGGGGTTTCCGGGGAGTTCAGGTACTTGGGCTTgtcgt                                             >  2:1928217/1‑150 (MQ=255)

                                                                                                              cAGGGCAATGACGTCCATGTAGCCCTCGACGACGATGATC‑TCGTCGAGGTTGCGGTTGTGCTTGCGCGCCTCGTACAGCCCGTACAGCTCCTGCCCCTTGTGGAAAACCGGGGTTTCCGGGGAGTTCAGGTACTTGGGCTTGTCGTCGcc                                         >  1:3283266/1‑150 (MQ=255)

                                                                                                                 ggCAATGACGTCCATGTAGCCCTCGACGACGATGATC‑TCGTCGAGGTTGCGGTTGTGCTTGCGCGCCTCGTACAGCCCGTACAGCTCCTGCCCCTTGTGGAAAACCGGGGTTTCCGGGGAGTTCAGGTACTTGGGCTTGTCGTCGCCGAg                                      >  2:2396076/1‑150 (MQ=255)

                                                                                                                       gACGTCCATGTAGCCCTCGACGACGATGATC‑TCGTCGAGGTTGCGGTTGTGCTTGCGCGCCTCGTACAGCCCGTACAGCTCCTGCCCCTTGTGGAAAACCGGGGTTTCCGGGGAGTTCAGGTACTTGGGCTTGTCGTCGCCGAGCACCCg                                <  2:4995867/150‑1 (MQ=255)

                                                                                                                           tCCATGTAGCCCTCGACGACGATGATC‑TCGTCGAGGTTGCGGTTGTGCTTGCGCGCCTCGTACAGCCCGTACAGCTCCTGCCCCTTGTGGAAAACCGGGGTTTCCGGGGAGTTCAGGTACTTGGGCTTGTCGTCGCCGAGCACCCGGCCa                            >  1:2981237/1‑150 (MQ=255)

                                                                                                                            ccATGTAGCCCTCGACGACGATGATC‑TCGTCGAGGTTGCGGTTGTGCTTGCGCGCCTCGTACAGCCCGTACAGCTCCTGCCCCTTGTGGAAAACCGGGGTTTCCGGGGAGTTCAGGTACTTGGGCTTGTCGTCGCCGAGCACCCGGCCAc                           >  1:4576078/1‑150 (MQ=255)

                                                                                                                               tGTAGCCCTCGACGACGATGATC‑TCGTCGAGGTTGCGGTTGTGCTTGCGCGCCTCGTACAGCCCGTACAGCTCCTGCCCCTTGTGGAAAACCGGGGTTTCCGGGGAGTTCAGGTACTTGGGCTTGTCGTCGCCGAGCACCCGGCCACCGa                        <  2:3543515/150‑1 (MQ=255)

                                                                                                                                gTAGCCCTCGACGACGGTGATC‑TCGTCGAGGTTGCGGTTGTGCTTGCGCGCCTCGTACAGCCCGTACAGCTCCTGCCCCTtgtg                                                                                         >  1:4952314/1‑84 (MQ=255)

                                                                                                                                gTAGCCCTCGACGACGATGATC‑TCGTCGAGGTTGCGGTTGTGCTTGCGCGCCTCGTACAGCCCGTACAGCTCCTGCCCCTTGTGGAAAACCGGGGTTTCCGGGGAGTTCAGGTACTTGGGCTTGTCGTCGCCGAGCACCCGGCCACCGaa                       >  1:3931059/1‑150 (MQ=255)

                                                                                                                                 tAGCCCTCGACGACGATGATC‑TCGTCGAGGTTGCGGTTGTGCTTGCGCGCCTCGTACAGCCCGTACAGCTCCTGCCCCTTGTGGAAAACCGGGGTTTCCGGGGAGTTCAGGTACTTGGGCTTGTCGTCGCCGAGCACCCGGCCACCGAAg                      >  1:674362/1‑150 (MQ=255)

                                                                                                                                   gCCCTCGACGACGATGATC‑TCGTCGAGGTTGCGGTTGTGCTTGCGCGCCTCGTACAGCCCGTACAGCTCCTGCCCCTTGTGGAAAACCGGGGTTTCCGGGGAGTTCAGGTACTTGGGCTTGTCGTCGCCGAGCACCCGGCCACCGAAGGc                    <  2:4939582/150‑1 (MQ=255)

                                                                                                                                     ccTCGACGACGATGATC‑TCGTCGAGGTTGCGGTTGTGCTTGCGCGCCTCGTACAGCCCGTACAGCTCCTGCCCCTTGTGGAAAACCGGGGTTTCCGGGGAGTTCAGGTACTTGGGCTTGTCGTCGCCGAGCACCCGGCCACCGAAGGCga                  <  1:5164112/150‑1 (MQ=255)

                                                                                                                                     ccTCGACGACGATGATC‑TCGTCGAGGTTGCGGTTGTGCTTGCGCGCCTCGTACAGCCCGTACAGCTCCTGCCCCTTGTGGAAAACCGGGGTTTCCGGGGAGTTCAGGTACTTGGGCTTGTCGTCGCCGAGCACCCGGCCACCGAAGGCga                  >  2:592491/1‑150 (MQ=255)

                                                                                                                                     ccTCGACGA‑GATCATCGTCGTCGAGGTTGCGGTTGTGCTTGCGCGCCTCGTACAGCCCGTACAGCTCCTGCCCCTTGTGGAAAACCgg                                                                                <  2:1597964/88‑1 (MQ=38)

                                                                                                                                      cTCGACGACGATGATC‑TCGTCGAGGTTGCGGTTGTGCTTGCGCGCCTCGTACAGCCCGTACAGCTCCTGCCCCTTGTGGAAAACCGGGGTTTCCGGGGAGTTCAGGTACTTGGGCTTGTCGTCGCCGAGCACCCGGCCACCGAAGGCgat                 <  1:2396076/150‑1 (MQ=255)

                                                                                                                                      cTCGACGA‑GATCATCGTCGTCGAGGTTGCGGTTGTGCTTGCGCGCCTCGTACAGCCCGTACAGCTCCTGCCCCTTGTGGAAAACCGGGGTTTCCGGGGAGTTCAGGTACTTGGGCTTGTCGTCGCCGAGCACCCGGCCACCGAAGGCgat                 >  1:1600892/1‑150 (MQ=255)

                                                                                                                                       tCGACGACGATGATC‑TCGTCGAGGTTGCGGTTGTGCTTGCGCGCCTCGTACAGCCCGTACAGCTCCTGCCCCTTGTGGAAAACCGGGGTTTCCGGGGAGTTCAGGTACTTGGGCTTGTCGTCGCCGAGCACCCGGCCACCGAAGGCgatg                >  2:3658484/1‑150 (MQ=255)

                                                                                                                                       tCGACGACGATGATC‑TCGTCGAGGTTGCGGTTGTGCTTGCGCGCCTCGTACAGCCCGTACAGCTCCTGCCCCTTGTGGAAAACCGGGGTTTCCGGGGAGTTCAGGTACTTGGGCTTGTCGTCGCCGAGCACCCGGCCACCGAAGGCgatg                <  2:3287308/150‑1 (MQ=255)

                                                                                                                                        cgacgacgaTGATC‑TCGTCGAGGTTGCGGTTGTGCTTGCGCGCCTCGTACAGCCCGTACAGCTCCTGCCCCTTGTGGAAAACCGGGGTTTCCGGGGAGTTCAGGTACTTGGGCTTGTCGTCGCCGAGCACCCGGCCACCGAAGGCgatga               <  1:1435221/150‑1 (MQ=255)

                                                                                                                                         gacgacgaTGATC‑TCGTCGAGGTTGCGGTTGTGCTTGCGCGCCTCGTACAGCCCGTACAGCTCCTGCCCCTTGTGGAAAACCGGGGTTTCCGGGGAGTTCAGGTACTTGGGCTTGTCGTCGCCGAGCACCCGGCCACCGAAGGCgatgat              <  1:4746530/150‑1 (MQ=255)

                                                                                                                                             acgaTGATC‑TCGTCGAGGTTGCGGTTGTGCTTGCGCGCCTCGTACAGCCCGTACAGCTCCTGCCCCTTGTGGAAAACCGGGGTTTCCGGGGAGTTCAGGTACTTGGGCTTGTCGTCGCCGAGCACCCGGCCACCGAAGGCGATGATgcgc          <  2:4411949/150‑1 (MQ=255)

                                                                                                                                               gatgatC‑TCGTCGAGGTTGCGGTTGTGCTTGCGCGCCTCGTACAGCCCGTACAGCTCCTGCCCCTTGTGGAAAACCGGGGTTTCCGGGGAGTTCAGGTACTTGGGCTTGTCGTCGCCGAGCACCCGGCCACCGAAGGCGATGATGCGccc        >  2:1787348/1‑150 (MQ=255)

                                                                                                                                               gatgatC‑TCGTCGAGGTTGCGGTTGTGCTTGCGCGCCTCGTACAGCCCGTACAGCTCCTGCCCCTTGTGGAAAACCGGGGTTTCCGGGGAGTTCAGGTACTTGGGCTTGTCGTCGCCGAGCACCCGGCCACCGAAGGCGATGATGCGccc        >  1:3736097/1‑150 (MQ=255)

                                                                                                                                               gatgatC‑TCGTCGAGGTTGCGGTTGCGCTTGCGCGCCTCGTACAGCCCGTACAGCTCCTGCCCCTTGTGGAAAACCGGGGTTTCCGGGGAGTTCAGGTACTTggg                                                     <  2:2263414/105‑1 (MQ=255)

                                                                                                                                                atgatC‑TCGTCGAGGTTGCGGTTGTGCTTGCGCGCCTCGTACAGCCCGTACAGCTCCTGCCCCTTGTGGAAAACCGGGGTTTCCGGGGAGTTCAGGTACTTGGGCTTGTCGTCGCCGAGCACCCGGCCACCGAAGGCGATGATGCGCCcg       >  1:379368/1‑150 (MQ=255)

                                                                                                                                                atgatC‑TCGTCGAGGTTGCGGTTGTGCTTGCGCGCCCCGTACAGCCCGTACAGCTCCTGCCCCTTGTGGAAAACCGGGGTTTCCGGGGAGTTCAGGTACTTGGGCTTGTCGTCGCCGAGCACCCGGCCACCGAAGGCGATGATGCGCCcg       >  1:546542/1‑150 (MQ=255)

                                                                                                                                                   atC‑TCGTCGAGGTTGCGGTTGTGCTTGCGCGCCTCGTACAGCCCGTACAGCTCCTGCCCCTTGTGGAAAACCGGGGTTTCCGGGGAGTTCAGGTACTTGGGCTTGTCGTCGCCGAGCACCCGGCCACCGAAGGCGATGATGCGCCCGCgg    >  1:2337047/1‑150 (MQ=255)

                                                                                                                                                   atC‑TCGTCGAGGTTGCGGTTGTGCTTGCGCGCCTCGTACAGCCCGTACAGCTCCTGCCCCTTGTGGAAAACCGGGGTTTCCGGGGAGTTCAGGTACTTGGGCTTGTCGTCGCCGAGCACCCGGCCACCGAAGGCGATGATGCGCCCGCgg    >  1:2336967/1‑150 (MQ=255)

                                                                                                                                                    tc‑tcGTCGAGGTTGCGGTTGTGCTTGCGCGCCTCGTACAGCCCGTACAGCTCCTGCCCCTTGTGGAAAACCggg                                                                               >  1:1092919/1‑74 (MQ=255)

                                                                                                                                                    tc‑tcGTCGAGGTTGCGGTTGTGCTTGCGCGCCTCGTACAGCCCGTACAGCTCCTGCCCCTTGTGGAAAACCGGGGTTTCCGGGGAGTTCAGGTACTTGGGCTTGTCGTCGCCGAGCACCCGGCCACCGAAGGCGATGATGCGCCCGCGGc   >  1:2211014/1‑150 (MQ=255)

CCGTCAAAACAGAACAGCACGCTGGGCACCACGCGGAACAGGCGCTTGAGGTGCTCTTCGCTGGTGGCGGTGCCAAGGGTGGCCACGGCATTGCGCAGGCCTTGCTGGGCCAGGGCAATGACGTCCATGTAGCCCTCGACGACGATGATC‑TCGTCGAGGTTGCGGTTGTGCTTGCGCGCCTCGTACAGCCCGTACAGCTCCTGCCCCTTGTGGAAAACCGGGGTTTCCGGGGAGTTCAGGTACTTGGGCTTGTCGTCGCCGAGCACCCGGCCACCGAAGGCGATGATGCGCCCGCGGCT  >  NC_002947/474053‑474351

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`