BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	6,332	C→A	6.8%	R17R (CGG→CGT)	trmE ←	GTPase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	6,332	0	C	A	6.8%	583.5 / 23.5	177	R17R (CGG→CGT)	trmE	GTPase
Reads supporting (aligned to +/- strand): ref base C (79/86); new base A (6/6); total (85/92)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.21e-01

Only 100 of 183 total aligned reads displayed.

AACAGAGCAATCCCTTCGTCCAGCACCAGCCCTTCATCGTCGCGGAATGGACCGTAATGGGCATGCCGCGGGGTCAGGGTGCGGCCGGTGATCAACAGGCCGGCCTTGGCAGCCAGAGGGCCGGACAACCTGACAATTCCCACACCGCCCCGGCCCTGGGCCGTGGCGATGGCGGCGATGGTTTCACGCACAGTGTTCATGCTCGAAAGCCTCTACGACAAAAACGACAGATAGCAAAAACGCCCCACTAGGGGGCGTTTTTTATTCACAGGCTAGCGTATTAGCCCGTCAAGCAGCA  >  NC_002947/6183‑6480

aaCAGAGCAATCCCTTCGTCCAGCACCAGCCATTCATCGTCGCGGAATGGACCGTAATGGGCATGCCGCGGGGTCAGGGTGCGGCCGGTGATCAACAGGCCGGCCTTGGCAGCCAGAGGGCCGGACAACCTGACAATTCCCACACCGccc                                                                                                                                                      >  2:2509486/1‑150 (MQ=255)

   agagCAATCCCTTCGTCCAGCACCAGCCCTTCATCGTCGCGGAATGGACCGTAATGGGCCTGCCGCGGGGTCAGGGTGCGGCCGGTGATCAACAGGCCGGCCTTGGCAGCTAGAGGGCCGGACAACCTGACAATTCCCACACCGCCCCgg                                                                                                                                                   >  1:4516019/1‑150 (MQ=255)

     agCAATCCCTTCGTCCAGCACCAGCCCTTCATCGTCGCGGAATGGACCGTAATGGGCATGCCGCGGGGTCAGGGTGCGGCCGGTGATCAACAGGCCGGCCTTGGCAGCCAGAGGGCCGGACAACCTGACAATTCCCACACCGCCCCGGcc                                                                                                                                                 <  1:5357310/150‑1 (MQ=255)

     agCAATCCCTTCGTCCAGCACCAGCCCTTCATCGTCGCGGAATGGACCGTAATGGGCATGCCGCGGGGTCAGGGTGCGGCCGGTGATCAACAGGCCGGCCTTGGCAGCCAGAGGGCCGGACAACCTGACAATTCCCACACCGCCCCGGcc                                                                                                                                                 <  1:5357301/150‑1 (MQ=255)

        aaTCCCTTCGTCCAGCACCAGCCCTTCATCGTCGCGGAATGGACCGTAATGGGCATGCCGCGGGGTCAGGGTGCGGCCGGTGATCAACAGGCCGGCCTTGGCAGCCAGAGGGCCGGACAACCTGACAATTCCCACACCGCCCCGGCCCTg                                                                                                                                              <  2:4027553/150‑1 (MQ=255)

             cTTCGTCCAGCACCAGCCCTTCATCGTCGCGGAATGGACCGTAATGGGCATGCCGCGGGGTCAGGGTGCGGCCGGTGATCAACAGGCCGGCCTTGGCAGCCAGAGGGCCGGACAACCTGACAATTCCCACACCGCCCCGGCCCTGGGCCg                                                                                                                                         >  1:4613212/1‑150 (MQ=255)

             cTTCGTCCAGCACCAGCCCTTCATCGTCGCGGAATGGACCGTAATGGGCATGCCGCGGGGTCAGGGTGCGGCCGGTGATCAACAGGCCGGCCTTGGCAGCCAGAGGGCCGGACAACCTGACAATTCCCACACCGCCCCGGCCCTGGGCCg                                                                                                                                         >  1:3086456/1‑150 (MQ=255)

             cTTCGTCCAGCACCAGCCCTTCATCGTCGCGGAATGGACCGTAATGGGCATGCCGCGGGGTCAGGGTGCGGCCGGTGATCAACAGGCCGGCCTTGGCAGCCAGAGGGCCGGACAACCTGACAATTCCCACACCGCCCCGGCCCTGGGCCg                                                                                                                                         <  1:2146608/150‑1 (MQ=255)

             cTTCGTCCAGCACCAGCCCTTCATCGTCGCGGAATGGACCGTAATGGGCATGCCGCGGGGTCAGGGTGCGGCCGGTGATCAACAGGCCGGCCTTGGCAGCCAGAGGGCCGGACAACCTGACAATTCCCACACCGCCCCGGCCCTGGGCCg                                                                                                                                         >  2:2404897/1‑150 (MQ=255)

              ttCGTCCAGCACCAGCCCTTCATCGTCGCGGAATGGACCGTAATTGGCATGCCGCGGGGTCAGGGTGCGGCCGGTGATCAACAGGCCGGCCTTGGCAGCCAGAGGGCCGGACAACCTGACAATTCCCACACCGCCCCGGCCCTGGGCCGt                                                                                                                                        >  1:4991800/1‑150 (MQ=255)

              ttCGTCCAGCACCAGCCCTTCATCGTCGCGGAATGGACCGTAATTGGCATGCCGCGGGGTCAGGGTGCGGCCGGTGATCAACAGGCCGGCCTTGGCAGCCAGAGGGCCGGACAACCTGACAATTCCCACACCGCCCCGGCCCTGGGCCGt                                                                                                                                        >  1:3859890/1‑150 (MQ=255)

              ttCGTCCAGCACCAGCCCTTCATCGTCGCGGAATGGACCGTAATGGGCATGCCGCGGGGTCAGGGTGCGGCCGGTGATCAACAGGCCGGCCTTGGCAGCCAGAGGGCCGGACAACCTGACAATTCCCACACCGCCCCGGCCCTGGGCCGt                                                                                                                                        >  1:3844639/1‑150 (MQ=255)

                cGTCCAGCACCAGCCCTTCATCGTCGCGGAATGGACCGTAATGGGCATGCCGCGGGGTCAGGGTGCGGCCGGTGATCAACAGGCCGGCCTTGGCAGCCAGAGGGCCGGACAACCTGACAATTCCCACATCGCCACGGCCCAGGGCCgggg                                                                                                                                      <  2:3595285/150‑1 (MQ=255)

                 gTCCAGCACCAGCCCTTCATCGTCGCGGAATGGACCGTAATGGGCATGCCGCGGGGTCAGGGTGCGGCCGGTGATCAACAGGCCGGCCTTGGCAGCCAGAGGGCCGGACAACCTGACAATTCCCACACCGCCCCGGCCCTGGGCCGTGGc                                                                                                                                     >  2:3478423/1‑150 (MQ=255)

                  tCCAGCACCAGCCCTTCATCGTCGCGGAATGGACCGTAATGGGCATGCCGCGGGGTCAGGGTGCGGCCGGTGATCAACAGGCCGGCCTTGGCAGCCAGAGGGCCGGACAACCTGACAATTCCCACACCGCCCCGGCCCTGGGCCGTGGCg                                                                                                                                    >  1:2204132/1‑150 (MQ=255)

                  tCCAGCACCAGCCCTTCATCGTCGCGGAATGGACCGTAATGGGCATGCCGCGGGGTCAGGGTGCGGCCGGTGATCAACAGGCCGGCCTTGGCAGCCAGAGGGCCGGACAACCTGACAATTCCCACACCGCCCCGGCCCTGGGCCGTGGCg                                                                                                                                    >  1:4012335/1‑150 (MQ=255)

                     aGCACCAGCCCTTCAGCGTCGCGGAATGGACCGTAATGGGCATGCCGCGGGGTCAGGGTGCGGCCGGTGATCAACAGGCCGGCCTTGGCAGCCAGAGGGCCGGACACCCTGACAATTCCCACACCGCCCCGGCCCGGGGCCGTGGCGATg                                                                                                                                 >  2:3346301/1‑150 (MQ=255)

                        aCCAGCCCTTCATCGTCGCGGAATGGACCGTAATGGGCATGCCGCGGGGTCAGGGTGCGGCCGGTGATCAACAGGCCGGCCTTGGCAGCCAGAGGGCCGGACAACCTGACAATTCCCACACCGCCACGGCCCAGGGCCGGGGCGGTgtgg                                                                                                                              <  1:4508904/150‑4 (MQ=255)

                          cAGCCCTTCATCGTCGCGGAATGGACCGTAATGGGCATGCCGCGGGGTCAGGGTGCGGCCGGTGATCAACAGGCCGGCCTTGGCAGCCAGAGGGCCGGACAACCTGACAATTCCCACACCGCCCCGGCCCTGGGCCGTGGCGATggcggc                                                                                                                            >  2:4477222/1‑150 (MQ=255)

                          cAGCCCTTCATCGTCGCGGAATGGACCGTAATGGGCATGCCGCGGGGTCAGGGTGCGGCCGGTGATCAACAGGCCGGCCTTGGCAGCCAGAGGGCCGGACAACCTGACAATTCCCACACCGCCCCGGCCCTGGGCCGTGGCGATggcggc                                                                                                                            <  2:3844639/150‑1 (MQ=255)

                           aGCCCTTCATCGTCGCGGAATGGACCGTAATGGGCATGCCGCGGGGTCAGGGTGCGGCCGGTGATCAACAGGCCGGCCTTGGCAGCCAGAGGGCCGGACAACCTGACAATTCCCACACCGCCCCGGCCCTGGGCCGTGGCGATGgcggcg                                                                                                                           >  1:2615665/1‑150 (MQ=255)

                            gCCCTTCATCGTCGCGGAATGGACCGTAATGGGCATGCCGCGGGGTCAGGGTGCGGCCGGTGATCAACAGGCCGGCCTTGGCAGCCAGAGGGCCGGACAACCTGACAATTCCCACACCGCCCCGGCCCTGGGCCGTGGCGATGGCGGCGa                                                                                                                          <  1:905498/150‑1 (MQ=255)

                            gCCCTTCATCGTCGCGGAATGGACCGTAATGGGCATGCCGCGGGGTCAGGGTGCGGCCGGTGATCAACAGGCCGGCCTTGGCAGCCAGAGGGCCGGACAACCTGACAATTCCCACACCGCCCCGGCCCTGGGCCGTGGCGATGGCGGCGa                                                                                                                          <  2:2615665/150‑1 (MQ=255)

                                      gtcgCGGAATGGACCGTAATGGGCATGCCGCGGGGTCAGGGTGCGGCCGGTGATCAACAGGCCGGCCTTGGCAGCCAGAGGGCCGGACAACCTGACAATTCCCACACCGCCCCGGCCCTGGGCCGTGGCGATGGCGGCGATGGTTTCACg                                                                                                                >  2:5333737/1‑150 (MQ=255)

                                       tcgCGGAATGCACCGTAATGGGCATGCCGCGGGGTCAGGGTGCGGCCGGTGAACAACAGGCCGTCCTAGGCAGCCAGACGGCCCGACAACCTGACAAATCCCACACCGCCCCGGCCCTGGGACGTGGCGATGGCGGCGAAGGttagatcg                                                                                                               >  2:681518/1‑144 (MQ=255)

                                        cgcgGAATGGACCGTAATGGGCATGCCGCGGGGTCAGGGTGCGGCCGGTGATCAACAGGCCGGCCTTGGCAGCCAGAGGGCCGGACAACCTGACAATTCCCACACCGCCCCGGCCCTGGGCCGTGGCGATGGCGGCGATGGTTTCACGca                                                                                                              <  1:1936370/150‑1 (MQ=255)

                                          cgGAATGGACCGTAATGGGCATGCCGCGGGGTCAGGGTGCGGCCGGTGATCAACAGGCCGGCCTTGGCAGCCAGAGGGCCGGACAACCTGACAATTCCCACACCGCCCCGGCCCTGGGCCGTGGCGATGGCGGCGATGGTTTCACGcaca                                                                                                            >  2:1442884/1‑150 (MQ=255)

                                           ggAATGGACCGTCATGGGCATGCCGCGGGGTCAGGGTGCGGCCGGTGATCAACAGGCCGGCCTTGGCAGCCAGAGGGCCGGACAACCTGACAATTCCCACACCGCCCCGGCCCTGGGCCGTGGCGATGGCGGCGATGGTTTCACGcaccg                                                                                                           >  2:5142704/1‑148 (MQ=255)

                                               tGGACCGTAATGGGCATGCCGCGGGGTCAGGGTGCGGCCGGTGATCAACAGGCCGGCCTTGGCAGCCAGAGGGCCGGACAACCTGACAAGTCCCACACCGCCCCGGCCCTGGGCCGTGGCGATGGCGGCGATGGTTTCACGCACAGTGtt                                                                                                       <  2:39432/150‑1 (MQ=255)

                                                 gACCGTAATGGGCATGCCGCGGGGTCAGGGTGCGGCCGGTGATCAACAGGCCGGCCTTGGCAGCCAGAGGGCCGGACAACCTGACAATTCCCACACCGCCCCGGCCCTGGGCCGTGGCGATGGCGGCGATGGTTTCACGCACAGTGTTCa                                                                                                     >  2:1517933/1‑150 (MQ=255)

                                                  aCCGTAATGGGCATGCCGCGGGGTCAGGGTGCGGCCGGTGATCAACAGGCCGGCCTTGGCAGCCAGAGGGCCGGACAACCTGACAATTCCCACACCGCCCCGGCCCTGGGCCGTGGCGATGGCGGCGATGGTTTCACGCACAGTGTTCAt                                                                                                    <  1:4807957/150‑1 (MQ=255)

                                                    cGTAATGGGCATGCCGCGGGGTCAGGGTGCGGCCGGTGATCAACAGGCCGGCCTTGGCAGCCAGAGGGCCGGACAACCTGACAATTCCCACACCGCCCCGGCCCTGGGCCGTGGCGATGGCGGCGATGGTTTCACGCACAGTGTTCATGc                                                                                                  >  1:2086298/1‑150 (MQ=255)

                                                      tAATGGGCATGCCGCGGGGTCAGGGTGCGGCCGGTGATCAACAGGCCGGCCTTGGCAGCCAGAGGGCCGGACAACCTGACAATTCCCACACCGCCCCGGCCCTGGGCCGTGGCGATGGCGGCGATGGTTTCACGCACAGTGTTCATGCTc                                                                                                <  2:4210631/150‑1 (MQ=255)

                                                        aTGGGCATGCCGCGGGGTCAGGGTGCGGCCGGTGATCAACAGGCCGGCCTTGGCAGCCAGAGGGCCGGACAACCTGACAATTCCCACACCGCCCCGGCCCTGGGCCGTGGCGATGGCGGCGATGGTTTCACGCACAGTGTTCATGCTCGa                                                                                              >  1:4704735/1‑150 (MQ=255)

                                                         tGGGCATGCCGCGGGGTCAGGGTGCGGCCGGTGATCAACAGGCCGGCCTTGGCAGCCAGAGGGCCGGACAACCTGACAATTCCCACACCGCCCCGGCCCTGGGCCGTGGCGATGGCGGCGATGGTTTCACGCACAGTGTTCATGCTCGaa                                                                                             <  2:4367665/150‑1 (MQ=255)

                                                         tGGGCAGGCCGCGGGGTCAGGGTGCGGCCGGTGATCAACAGGCCGGCCTTGGCAGCCAGAGGGCCGGACAACCTGACAATTCCCACACCGCCCCGGCCCTGGGCCGTGGCGATGGCGGCGATGGTTTCACGCACAGTGTTCATGCTCGaa                                                                                             <  1:5244722/150‑1 (MQ=255)

                                                          gggCATGCCGCGGGGTCAGGGTGCGGCCGGTGATCAACAGGCCGGCCTTGGCAGCCAGAGGGCCGGACAACCTGACAATTCCCACACCGCCCCGGCCCTGGGCCGTGGCGATGGCGGCGATGGTTTCACGCACAGTGTTCATGCTCGaaa                                                                                            <  1:1481201/150‑1 (MQ=255)

                                                          gggCATGCCGCGGGGTCAGGGTGCGGCCGGTGATCAACAGGCCGGCCTTGGCAGCCAGAGGGCCGGACAACCTGACAATTCCCACACCGCCCCGGCCCTGGGCCGTGGCGATGGCGGCGATGGTTTCACGCACAGTGTTCATGCTCGaaa                                                                                            <  1:1088224/150‑1 (MQ=255)

                                                          gggCATGCCGCGGGGTCAGGGTGAGGCCGGTGATCAACAGGCCGGCCTTGGCAGCCAGAGGGCCGGACAACCTGACAATTCCCACACCGCCCCGGCCCTGGGCCGTGGCGATGGCGGCGATGGTTTCACGCACAGTGTTCATGCTCGaaa                                                                                            <  2:1695598/150‑1 (MQ=255)

                                                              aTGCCGCGGGGTCAGGGTGCGGCCGGTGATCAACAGGCCGGCCTTGGCAGCCAGAGGGCCGGACAACCGGACAATTCCCACACCGCCCCGGCCCTGGGCCGTGGCGATGGCGGCGATGGTTTCACGCACAGTGTTCATGCTCGAAAGCct                                                                                        <  2:3764394/150‑1 (MQ=255)

                                                               tGCCGCGGGGTCAGGGTGCGGCCGGTGATCAACAGGCCGGCCTTGGCAGCCAGAGGGCCGGACAACCTGACAATTCCCACACCGCCCCGGCCCTGGGCCGTGGCGATGGCGGCGATGGTTTCACGCACAGTGTTCATGCTCGAAAGCctc                                                                                       <  2:1363605/150‑1 (MQ=255)

                                                                gCCGCGGGGTCAGGGTGCGGCCGGTGATCAACAGGCCGGCCTTGGCAGCCAGAGGGCCGGACAACCTGACAATTCCCACACCGCCCCGGCCCTGGGCCGTGGCGATGGCGGCGATGGTTTCACGCACAGTGTTCATGCTCGAAAGCctct                                                                                      <  2:3064042/150‑1 (MQ=255)

                                                                 ccGCGGGGTCAGGGTGCGGCCGGTGATCAACAGGCCGGCCTTGGCAGCCAGAGGGCCGGACAACCTGACAATTCCCACACCGCCCCGGCCCTGGGCCGTGGCGATGGCGGCGATGGTTTCACGCACAGTGTTCATGCTCGAAAGCCTCTa                                                                                     <  1:4292402/150‑1 (MQ=255)

                                                                 ccGCGGGGTCAGGGTGCGGCCGGTGATCAACAGGCCGGCCTTGGCAGCCAGAGGGCCGGACAACCTGACAATTCCCACACCGCCCCGGCCCTGGGCCGTGGCGATGGCGGCGATGGTTTCACGCACAGTGTTCATGCTCGAAAGCCTCTa                                                                                     <  1:1569631/150‑1 (MQ=255)

                                                                  cgcgGGGTCAGGGTGCGGCCGGTGATCAACAGGCCGGCCTTGGCAGCCAGAGGGCCGGACAACCTGACAATTCCCACACCGCCCCGGCCCTGGGCCGTGGCGATGGCGGCGATGGTTTCACGCACAGTGTTCATGCTCGAAAGCCTCTAc                                                                                    <  1:2566140/150‑1 (MQ=255)

                                                                        gTCAGGGTGCGGCCGGTGATCAACAGGCCGGCCTTGGCAGCCAGAGGGCCGGACAACCTGACAATTCCCACACCGCCCCGGCCCTGGGCCGTGGCGATGGCGGCGATGGTTTCACGCACAGTGTTCAt                                                                                                    >  1:16892/1‑128 (MQ=255)

                                                                        gTCAGGGTGCGGCCGGTGATCAACAGGCCGGCCTTGGCAGCCAGAGGGCCGGACAACCTGACAATTCCCACACCGCCCCGGCCCTGGGCCGTGGCGATGGCGGCGATGGTTTCACGCACAGTGTTCATGCTCGAAAGCCTCTACGACaaa                                                                              >  2:1935912/1‑150 (MQ=255)

                                                                         tCAGGGTGCGGCCGGTGATCAACAGGCCGGCCTTGGCAGCCAGAGGGCCGGACAACCTGACAATTCCCACACCGCCCCGGCCCTGGGCCGTGGCGATGGCGGCGATGGTTTCACGCACAGTGTTCATGCTCGAAAGCCTCTACGACaaaa                                                                             <  2:4121210/150‑1 (MQ=255)

                                                                             ggTGCGGCCGGTGATCAACAGGCCGGCCTTGGCAGCCAGAGGGCCGGACAACCTGACAATTCCCACACCGCCCCGGCCCTGGGCCGTGGCGATGGCGGCGATGGTTTCACGCACAGTGTTCATGCTCGAAAGCCTCTACGACAAAAACGa                                                                         <  2:2187446/150‑1 (MQ=255)

                                                                             ggTGCGGCCGGTGATCAACAGGCCGGCCTTGGCAGCCAGAGGGCCGGACAACCTGACAATTCCCACACCGCCCCGGCCCTGGGCCGTGGCGATGGCGGCGATGGTTTCACGCACAGTGTTCATGCTCGAAAGCCTCTACGACAAAAACGa                                                                         >  1:993971/1‑150 (MQ=255)

                                                                               tGCGGCCGGTGATCAACAGGCCGGCCTTGGCAGCCAGAGGGCCGGACAACCTGACAATTCCCACACCGCCCCGGCCCTGGGCCGTGGCGATGGCGGCGATGGTTTCACGCACAGTGTTCATGCTCGAAAGCCTCTACGACAAAAACGACa                                                                       <  2:5046070/150‑1 (MQ=255)

                                                                               tGCGGCCGGTGATCAACAGGCCGGCCTTGGCAGCCAGAGGGCCGGACAACCTGACAATTCCCACACCGCCCCGGCCCTGGGCCGTGGCGATGGCGGCGATGGTTTCACGCACAGTGTTCATGCTCGAAAGCCTCTACGACAAAAACGACa                                                                       <  1:1951122/150‑1 (MQ=255)

                                                                                  ggCCGGTGATCAACAGGCCGGCCTTGGCAGCCAGAGGGCCGGACAACCTGACAATTCCCACACCGCCCCGGCCCTGGGCCGTGGCGATGGCGGCGATGGTTTCACGCACAGTGTTCATGCTCGAAAGCCTCTACGACAAAAACGACAGAt                                                                    >  2:4661715/1‑150 (MQ=255)

                                                                                   gCCGGTGATCAACTGGCCGGCCTTGGCAGCCAGAGGGCCGGACAACCTGACAATTCCCACACCGCCCCGGCACTGGGCCGTGGAGATGGCGGCTATGGTTTCACGCACAGTGTTCATGCTCGAAAGCCTCTACGACAAAAACGACAGATa                                                                   <  2:300333/150‑1 (MQ=255)

                                                                                        tGATCAACAGGCCGGCCTTGGCAGCCAGAGGGCCGGACAACCTGACAATTCCCACACCGCCCCGGCCCTGGGCCGTGGCGATGGCGGCGATGGTTTCACGCACAGTGTTCATGCTCGAAAGCCTCTACGACAAAAACGACAGATAGCaaa                                                              >  1:5207882/1‑150 (MQ=255)

                                                                                        tGATCAACAGGCCGGCCTTGGCAGCCAGAGGGCCGGACAACCTGACAATTCCCACACCGCCCCGGCCCTGGGCCGTGGCGATGGCGGCGATGGTTTCACGCACAGTGTTCATGCTCGAAAGCCTCTACGACAAAAACGACAGATAGCaaa                                                              >  1:1585606/1‑150 (MQ=255)

                                                                                           tCAACAGGCCGGCCTTGGCAGCCAGAGGGCCGGACAACCTGACAATTCCCACACCGCCCCGGCCCTGGGCCGTGGCGATGGCGGCGATGGTTTCACGCACAGTGTTCATGCTCGAAAGCCTCTACGACAAAAACGACa                                                                       >  2:3104596/1‑138 (MQ=255)

                                                                                           tCAACAGGCCGGCCTTGGCAGCCAGAGGGCCGGACAACCTGACAATTCCCACACCGCCCCGGCCCTGGGCCGTGGCGATGGCGGCGATGGTTTCACGCACAGTGTTCATGCTCGAAAGCCTCTACGACAAAAACGACAGATAGCAAAAAc                                                           >  1:944222/1‑150 (MQ=255)

                                                                                           tCAACAGGCCGGCCTTGGCAGCCAGAGGGCCGGACAACCTGACAATTCCCACACCGCCCCGGCCCTGGGCCGTGGCGATGGCGGCGATGGTTTCACGCACAGTGTTCATGCTCGAAAGCCTCTACGACAAAAACGACAGATAGCAAAAAc                                                           >  1:3686521/1‑150 (MQ=255)

                                                                                            cAACAGGCCGGCCTTGGCAGCCAGAGGGCCGGACAACCTGACAATTCCCACACCGCCCCGGCCCTGGGCCGTGGCGATGGCGGCGATGGTTTCACGCACAGTGTTCATGCTCGAAAGCCTCTACGACAAAAACGACAGATAGCAAAAACg                                                          >  2:3606097/1‑150 (MQ=255)

                                                                                             aaCAGGCCGGCCTTGGCAGCTAGAGGGCCGGACAACCTGACAATTCCCACACCGCCCCGGCCCTGGGCCGTGGCGATGGCGGCGATGGTTTCACGCACAGTGTTCATGCTCGAAAGCCTCTACGACAAAAACGACAGATAGCAAAAACGc                                                         <  2:4516019/150‑1 (MQ=255)

                                                                                             aaCAGGCCGGCCTTGGCAGCCAGAGGGCCGGACAACCTGACAATTCCCACACCGCCCCGGCCCTGGGCCGTGGCGATGGCGGCGATGGTTTCACGCACAGTGTTCATGCTCGAAAGCCTCTACGACAAAAACGACAGATAGCAAAAACGc                                                         >  1:365736/1‑150 (MQ=255)

                                                                                                  gccggccTTGGCAGCCAGAGGGCCGGACAACCTGACAATTCCCACACCGCCCCGGCCCTGGGCCGTGGCGATGGCGGCGATGGTTTCATGCACAGTGTTCATGCTCGAAAGCCTCTACGACAAAAACGACAGATAGCAAAAACGCCCCAc                                                    >  1:710702/1‑150 (MQ=255)

                                                                                                    cggccTTGGCAGCCAGAGGGCCGGACAACCTGACAATTCCCACACCGCCCCGGCCCTGGGCCGTGGCGATGGCGGCGATGGTTTCACGCACAGTGTTCATGCTCGAAAGCCTCTACGACAAAAACGACAGATAGCAAAAACGCCCCACTa                                                  >  1:1304005/1‑150 (MQ=255)

                                                                                                     ggccTTGGCAGCCAGAGGGCCGGACAACCTGACAATTCCCACACCGCCACGGCCCAGGGCCGGGGCggt                                                                                                                                  <  1:4195435/69‑3 (MQ=37)

                                                                                                       ccTTGGCAGCCAGAGGGCCGGACAACCTGACAATTCCCACACCGCCCCGGCCCTGGGCCGTGGCGATGGCGGCGATGGTTTCACGCACAGTGTTCATGCTCGAAAGCCTCTACGACAAAAACGACAGATAGCAAAAACGCCCCACTAggg                                               <  2:5173875/150‑1 (MQ=255)

                                                                                                       ccTTGGCAGCCAGAGGGCCGGACAACCTGACAATTCCCACACCGCCCCGGCCCTGGGCCGTGGCGATGGCGGCGATGGTTTCACGCACAGTGTTCATGCTCGAAAGCCTCTACGACAAAAACGACAGATAGCAAAAACGCCCCACTAggg                                               <  2:1618262/150‑1 (MQ=255)

                                                                                                              aGCCAGAGGGCCGGACAACCTGACAATTCCCACACCGCCCCGGCCCTGGGCCGTGGCGATGGCGGCGATGGTTTCACGCACAGTGTTCATGCTCGAAAGCCTCTACGACAAAAACGACAGAt                                                                    <  2:839641/122‑1 (MQ=255)

                                                                                                              aGCCAGAGGGCCGGACAACCTGACAATTCCCACACCGCCCCGGCCCTGGGCCGTGGCGATGGCGGCGATGGTTTCACGCACAGTGTTCATGCTCGAAAGCCTCTACGACAAAAACGACAGAt                                                                    >  1:839641/1‑122 (MQ=255)

                                                                                                               gCCAGAGGGCCGGACAACCTGACAATTCCCACACCGCCCCGGCCCTGGGCCGTGGCGATGGCGGCGATGGTTTCACGCACAGTGTTCATGCTCGAAAGCCTCTACGACAAAAACGACAGATAGCAAAAACGCCCCACTAGGGGGCGtttt                                       <  2:4671707/150‑1 (MQ=255)

                                                                                                                ccAGCGGGCCGGACAACCTGACAATTCCCACACCGCCCCGGCCCTGGGCGGTGGCGATGGCGGCGATGGTTTCACGCACAGTGTTCATGCTCGAAAGCCTCTACGACAAAAACGACAGATAGCAAAAACGCCCCACTAGGGGGCGttttt                                      <  1:620321/150‑1 (MQ=255)

                                                                                                                ccAGAGGGCCGGACAACCTGACAATTCCCACACCGCCCCGGCCCTGGGCCGTGGCGATGGCGGCGATGGTTTCACGCACAGTGTTCATGCTCGAAAGCCTCTACGACAAAAACGACAGATAGCAAAAACGCCCCACTAGGGGGCGttttt                                      <  2:2770303/150‑1 (MQ=255)

                                                                                                                 cAGAGGGCCGGACAACCTGACAATTCCCACACCGCCCCGGCCCTGGGCCGTGGCGATGGCGGCGATGGTTTCACGCACAGTGTTCATGCTCGAAAGCCTCTACGACAAAAACGACAGATAGCAAAAACGCCCCACTAGGGGGCGtttttt                                     <  1:3605488/150‑1 (MQ=255)

                                                                                                                   gagGGCCGGACAACCTGACAATTCCCACACCGCCCCGGCCCTGGGCCGTGGCGATGGCGGCGATGGTTGCACGCACAGTGTTCATGCTCGAAAGCCTCTACGACCAACACGACCGATAGCAAAAACGCCCCACTAGGGGGCGTTTTTTAt                                   <  2:2547956/150‑1 (MQ=255)

                                                                                                                    cgggCCGGACACCCTGACACTGCCCACCCCGCCCCGGCCGTGGGCCGGGGCGCTGGCGGCGATGGTTTCCCGCACCGTGTTCATGCTCGAACGCCTCTACGACAAAAACGACCGATCGCACAAACGCCCCACTAGGGGGCGTTTTTTAtt                                  <  1:5142704/149‑1 (MQ=255)

                                                                                                                    cgggCCGGACAACCGGACAATGCCCCCCCCGCCCCGGCCCTGGGCCGTGGCGATGGCGGCGATGGTTTCACGCACAGTGTTCATGCTCGAAAGCCTCTACGACAAAAACGACAGATAGCAAAAACGCCCCACTAGGGGGCGTTTTTTAtt                                  <  1:3344908/149‑1 (MQ=255)

                                                                                                                      ggCCGGACAACCGGACAATTCCCCCCCCGCCCCGGCCCTGGGCCGTGGCGATGGCGGCGATGGTTTCACGCACAGTGTTCAGGCTCGAACGCCTCTACGCCAAAAACGACCGATAGCAAAAACGCCCCACTAGGGGGCGTTTTTTATTca                                <  2:2340099/150‑1 (MQ=255)

                                                                                                                        ccGGACAACCTGACAATTCCCACACCGCCCCGGCCCTGGGCCGTGGCGATGGCGGCGATGGTTTCACGCACAGTGTTCATGCTCGAAAGCCTCAACGACAAAAACGACAGATAGCAAAAACTCCCCACTAGGGGGCGTTTTTTATTcaca                              >  1:3775353/1‑150 (MQ=255)

                                                                                                                        ccGGACAACCTGACAATCCCCCCCCCGCCCCGGCCCTGGGCCGTGGCGAGGGCGGCGATGGTTTCACGCACCGTGTCCAGGCTCGAAAGCCACTACGACAAAAACGACAGATAGCAAAAACGCCCCACTAGGGGGCGTTTTTTATTcaca                              <  1:2509486/150‑1 (MQ=255)

                                                                                                                        ccGGACAACCTGACAATACCCACACCGCCCCGGCCCTGGGCCGGGGCGAGGGCGGCGATGGTTTCACGCACAGTGTTCATGCTCGAAAGCCTCTCCGACAAAAACGACAGATAGCAAAAACGCCCCACTAGGGGGCGATTTTTATTcaca                              <  2:4628199/150‑1 (MQ=255)

                                                                                                                         cGGACAACCTGACAATTCCCACACCGCCCCGGCCCTGGGCCGTGGCGATGGCGGCGATGGTTTCACGCACAGTGTTCATGCTCGAAAGCCTCTACGACAAAAACGACAGATAGCAAAAACGCCCCACTAGGGGGCGTTTTTTATTCACAg                             >  2:194136/1‑150 (MQ=255)

                                                                                                                          ggACAACCTTACACTCCCCACCCCGCCCCGGCCCTGGGCCGTGGCGATGGCGGCGATGGTTTCACGAACAGTTTTCCTGCTCGAAAGCCTCTACTACAAAAACGACAGATAGCAAAAACGCCCCACTAGGGGGCGTTTTTTATTCACAgg                            <  1:2302065/150‑1 (MQ=255)

                                                                                                                            aCAACCTGACAATTCCCACACCGCCCCGGCCCTGGGCCGTGGCGATGGCGGCGATGGTTTCACGCACAGTGTTCATGCTCGAAAGCCTCTACGACAAAAACGACAGATAGCAAAAACGCCCCACTAGGGGGCGTTTTTTATTCACAGGCt                          >  2:2535062/1‑150 (MQ=255)

                                                                                                                             cAACCTGACAATTCCCACACCGCCCCGGCCCTGGGCCGTGGCGATGGCGGCGATGGTTTCACGCACAGTGTTCATGCTCGAAAGCCTCTACGACAAAAACGACAGATAGCAAAAACGCCCCACTAGGGGGCGTTTTTTATTCACAGGCTa                         <  1:2404457/150‑1 (MQ=255)

                                                                                                                               aCCTGACAATTCCCACACCGCCCCGGCCCTGGGCCGTGGCGATGGCGGCGATGGTTTCACGCACAGTGTTCATGCTCGAAAGCCTCTACGACAAAAACGACAGATAGCAAAAACGCCCCACTAGGGGGCGTTTTTTATTCACAGGCTAGc                       >  2:4263868/1‑150 (MQ=255)

                                                                                                                               aCCTGACAATTCCCACACCGCCCCGGCCCTGGGCCGTGGCGATGGCGGCGATGGTTTCACGCACAGTGTTCATGCTCGAAAGCCTCTACGACAAAAACGACAGATAGCAAAAACGCCCCACTAGGGGGCGTTTTTTATTCACAGGCTAGc                       <  2:1585606/150‑1 (MQ=255)

                                                                                                                                   gACAATTCCCACACCGCCCCGGCCCTGGGCCGTGGCGATGGCGGCGATGGTTTCACGCACAGTGTTCATGCTCGAAAGCCTCTACGACAAAAACGACAGATAGCAAAAACGCCCCACTAGGGGGCGTTTTTTATTCACAGGCTAGCGTAt                   >  1:139783/1‑150 (MQ=255)

                                                                                                                                     cAATTCCCACACCGCCCCGGCCCGGGGCCGTGGCGATGGCGGCTATGGTTTCACGCACAGTTTTAATGCTCGAAAGCCCCAACCACAAAAACGACAGATAGCAAAAACGCCCCACTAGGGGGCGTTTTTTATTCACAGGCTAGCGTATTa                 <  1:2346604/150‑1 (MQ=255)

                                                                                                                                        ttCCCACACCGCCCCGGCCCTGGGCCGTGGCGATGGCGGCGATGGTTTCACGCACAGTGTTCATGCTCGAAAGCCTCTACGACAAAAACGACAGATAGCAAAAACGCCCCACTAGGGGGCGTTTTTTATTCACAGGCTAGCGTATTAGcc              <  2:2398067/150‑1 (MQ=255)

                                                                                                                                          cccACACCGCCCCGGCCCTGGGCCGTGGCGATGGCGGCGATGGTTTCACGCACAGTGTTCATGCTCGAAAGCCTCTACGACAAAAACGACAGATAGCAAAAACGCCCCACTAGGGGGCGTTTTTTATTCACAGGCTAGCGTATTAGCCCg            >  1:4480996/1‑150 (MQ=255)

                                                                                                                                            cacaCCGCCCCGGCCCTGGGCCGTGGCGATGGCGGCGATGGTTTCACGCACAGTGTTCATGCTCGAAAGCCTCTACGACAAAAACGACAGATAGCAAAAACGCCCCACTAGGGGGCGTTTTTTATTCACAGGCTAGCGTATTAGCCCGTc          >  2:1667885/1‑150 (MQ=255)

                                                                                                                                            cacaCCGCCCCGGCCCTGGGCCGTGGCGATGGCGGCGATGGTTTCACGCACAGTGTTCATGCTCGAAAGCCTCTACGACAAAAACGACAGATAGCAAAAACGCCCCACTAGGGGGCGTTTTTTATTCACAGGCTAGCGTATTAGCCCGTc          >  2:3527950/1‑150 (MQ=255)

                                                                                                                                             acacCGCCCCGGCCCTGGGCCGTGGCGATGGCGGCGATGGTTTCACGCACAGTGTTCATGCTCGAAAGCCTCTATGACAAAAACGACAGATAGCAAAAACGCCCCACTAGGGGGCGTTTTTTATTCACAGGCTAGCGTATTAGCCCGTCa         >  2:4815617/1‑150 (MQ=255)

                                                                                                                                                tcGCCACGGCCCAGGGCCGGGGCGGTGGCGGCGATGGTTTCACGCACAGTGTTCATGCTCGAAAGCCTCTACGACAAAAACGACAGATAGCAAAAACGCCCCACTAGGGGGCGTTTTTTATTCACAGGCTAGCGTATTAGCCCGTCAagc      >  2:4539370/2‑150 (MQ=255)

                                                                                                                                                tcGCCACGGCCCAGGGCCGGGGCGGTGGCGGCGATGGTTTCACGCACAGTGTTCATGCTCGAAAGCCTCTACGACAAAAACGACAGATAGCAAAAACGCCCCACTAGGGGGCGTTTTTTATTCACAGGCTAGCGTATTAGCCCGTCAagc      >  1:2882744/2‑150 (MQ=255)

                                                                                                                                                ccGCCCCGGCCCTGGGCCGTGGCGATGGCGGCGATGGTTTCACGCACAGTGTTCATGCTCGAAAGCCTCTACGACAAAAACGACAGATAGCAAAAACGCCCCACTAGGGGGCGTTTTTTATTCACAGGCTAGCGTATTAGCCCGTCAagc      >  2:485164/1‑150 (MQ=255)

                                                                                                                                                 cGCCCCGGCCCTGGGCCGTGGCGATGGCGGCGATGGTTTCACGCACAGTGTTCATGCTCGAAAGCCTCTACGACAAAAACGACAGATAGCAAAAACGCCCCACTAGGGGGCGTTTTTTATTCACAGGCTAGCGTATTAGCCCGTCAagca     >  2:2274036/1‑150 (MQ=255)

                                                                                                                                                 cGCCCCGGCCCTGGGCCGTGGCGATGGCGGCGATGGTTTCACGCACAGTGTTCATGCTCGAAAGCCTCTACGACAAAAACGACAGATAGCAAAAACGCCCCACTAGGGGGCGTTTTTTATTCACAGGCTAGCGTATTAGCCCGTCAagca     >  1:1644734/1‑150 (MQ=255)

                                                                                                                                                   ccccGGCCCTGGGCCGTGGCGATGGCGGCGATGGTTTCACGCACAGTGTTCATGCTCGAAAGCCTCTACGACAAAAACGACAGATAGCAAAAACGCCCCACTAGGGGGCGTTTTTTATTCACAGGCTAGCGTATTAGCCCGTCAagcagc   <  2:5216403/150‑1 (MQ=255)

                                                                                                                                                    cccGGCCCTGGGCCGTGGCGATGGCGGCGATGGTTTCACGCACAGTGTTCATGCTCGAAAGCCTCTACGACAAAAACGACAGATAGCAAAAACGCCCCACTAGGGGGCGTTTTTTATTCACAGGCTAGCGTATTAGCCCGTCAagcagca  >  2:2371285/1‑150 (MQ=255)

AACAGAGCAATCCCTTCGTCCAGCACCAGCCCTTCATCGTCGCGGAATGGACCGTAATGGGCATGCCGCGGGGTCAGGGTGCGGCCGGTGATCAACAGGCCGGCCTTGGCAGCCAGAGGGCCGGACAACCTGACAATTCCCACACCGCCCCGGCCCTGGGCCGTGGCGATGGCGGCGATGGTTTCACGCACAGTGTTCATGCTCGAAAGCCTCTACGACAAAAACGACAGATAGCAAAAACGCCCCACTAGGGGGCGTTTTTTATTCACAGGCTAGCGTATTAGCCCGTCAAGCAGCA  >  NC_002947/6183‑6480

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 11 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`