BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	1,549,879	C→G	13.5%	S79S (TCC→TCG)	groL →	chaperonin GroEL

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	1,549,879	0	C	G	13.5%	594.5 / 73.0	207	S79S (TCC→TCG)	groL	chaperonin GroEL
Reads supporting (aligned to +/- strand): ref base C (91/88); new base G (19/9); total (110/97)
Fisher's exact test for biased strand distribution p-value = 1.06e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.04e-01

Only 100 of 212 total aligned reads displayed.

CCTGGGCCCGAAAGGCCGTAACGTGGTACTGGCCAAGAGCTTCGGCGCGCCGACCATCACCAAGGACGGCGTTTCCGTCGCCAAAGAAATCGAGCTGAAAGACGCTTTCGAAAACATGGGCGCCCAGCTGGTCAAGGAAGTTGCTTCCAAGGCCAACGACGCTGCCGGTGACGGCACCACCACCGCTACCGTTCTGGCTCAGGCCATCGTCAACGAAGGCCTGAAAGCCGTCGCTGCCGGCATGAACCCGATGGACCTGAAGCGCGGTATCGACAAGGCCACTGCTGCCGTCGTTGC  >  NC_002947/1549732‑1550028

ccTGGGCCCGAAAGGCCGTAACGTGGTACTGGCCAAGAGTTTCGGCGCGCCGACCATCACCAAGGACGGCGTTTCCGTCGCCAAAGAAATCGAGCTGAAAGACGCTTTCGAAAACATGGGCGCCCAGCTGGTCAAGGAAGTTGCTTCCaa                                                                                                                                                     >  2:3614934/1‑150 (MQ=255)

ccTGGGCCCGAAAGGCCGTAACGTGGTACTGGCCAAGAGCTTCGGCGCGCCGACCATCACCAAGGACGGCGTTTCCGTCGCCAAAGAAATCGAGCTGAAAGACGCTTTCGAAAACATGGGCGCCCAGCTGGTCAAGGAAGTTGCTTCCaa                                                                                                                                                     <  2:4703523/150‑1 (MQ=255)

      cccGAAAGGCCGTAACGTGGTACTGGCCAAGAGCTTCGGCGCGCCGACCATCACCAAGGACGGCGTTTCCGTCGCCAAAGAAATCGAGCTGAAAGACGCTTTCGAAAACATGGGCGCCCAGCTGGTCAAGGAAGTTGCTTCCAAGGCCaa                                                                                                                                               <  1:2130657/150‑1 (MQ=255)

       ccGAAAGGCCGTAACGTGGTACTGGCCAAGAGCTTCGGCGCGCCGACCATCACCAAGGACGGCGTTTCCGTCGCCAAAGAAATCGAGCTGAAAGACGCTTTCGAAAACATGGGCGCCCAGCTGGTCAAGGAAGTTGCTTCCAAGGCCAac                                                                                                                                              >  1:4015790/1‑150 (MQ=255)

       ccGAAAGGCCGTAACGTGGTACTGGCCAAGAGCTTCGGCGCGCCGACCATCACCAAGGACGGCGTTTCCGTCGCCAAAGAAATCGAGCTGAAAGACGCTTTCGAAAACATGGGCGCCCAGCTGGTCAAGGAAGTTGCTTCCAAGGCCAac                                                                                                                                              <  1:455438/150‑1 (MQ=255)

       ccGAAAGGCCGTAACGTGGTACTGGCCAAGAGCTTCGGCGCGCCGACCATCACCAAGGACGGCGTTTCCGTCGCCAAAGAAATCGAGCTGAAAGACGCTTTCGAAAACATGGGCGCCCAGCTGGTCAAGGAAGTTGCTTCCAAGGCCAac                                                                                                                                              <  1:272800/150‑1 (MQ=255)

       ccGAAAGGCCGTAACGTGGTACTGGCCAAGAGCTTCGGCGCGCCGACCATCACCAAGGACGGCGTTTCCGTCGCCAAAGAAATCGAGCTGAAAGACGCTTTCGAAAACATGGGCGCCCAGCTGGTCAAGGAAGTTGCTTCCAAGGCCAac                                                                                                                                              <  2:1335135/150‑1 (MQ=255)

       ccGAAAGGCCGTAACGTGGTACTGGCCAAGAGCTTCGGCGCGCCGACCATCACCAAGGACGGCGTTTCCGTCGCCAAAGAAATCGAGCTGAAAGACGCTTTCGAAAACATGGGCGCCCAGCTGGTCAAGGAAGTTGCTTCCAAGGCCAac                                                                                                                                              <  1:3168204/150‑1 (MQ=255)

           aaGGCCGTAACGTGGTACTGGCCAAGAGCTTCGGCGCGCCGACCATCACCAAGGACGGCGTTTCCGTCGCCAAAGAAATCGAGCTGAAAGACGCTTTCGAAAACATGGGCGCCCAGCTGGTCAAGGAAGTTGCTTCCAAGGCCAacgacg                                                                                                                                          <  1:1066322/150‑1 (MQ=255)

                cGTAACGTGGTACTGGCCAAGAGCTTCGGCGCGCCGACCATCACCAAGGACGGCGTTTCCGTCGCCAAAGAAATCGAGCTGAAAGACGCTTTCGAAAACATGGGCGCCCAGCTGGTCAAGGAAGTTGCTTCCAAGGCCAACGACGCTGcc                                                                                                                                     <  2:3333790/150‑1 (MQ=255)

                 gTAACGTGGTACTGGCCAAGAGCTTCGGCGCGCCGACCATCACCAAGGACGGCGTTTCCGTCGCCAAAGAAATCGAGCTGAAAGACGCTTTCGAAAACATGGGCGCCCAGCTGGTCAAGGAAGTTGCTTCCAAGGCCAACGACGCTGCCg                                                                                                                                    <  2:4389113/150‑1 (MQ=255)

                 gTAACGTGGTACTGGCCAAGAGCTTCGGCGCGCCGACCATCACCAAGGACGGCGTTTCCGTCGCCAAAGAAATCGAGCTGAAAGACGCTTTCGAAAACATGGGCGCCCAGCTGGTCAAGGAAGTTGCTTCCAAGGCCAACGACGCTGCCg                                                                                                                                    <  1:1824792/150‑1 (MQ=255)

                  tAACGTGGTACTGGCCAAGAGCTTCGGCGCGCCGACCATCACCAAGGACGGCGTTTCCGTCGCCAAAGAAATCGAGCTGAAAGACGCTTTCGAAAACATGGGCGCCCAGCTGGTCAAGGAAGTTGCTTCCAAGGCCAACGACGCTGCCgg                                                                                                                                   <  2:4469905/150‑1 (MQ=255)

                  tAACGTGGTACTGGCCAAGAGCTTCGGCGCGCCGACCATCACCAAGGACGGCGTTTCCGTCGCCAAAGAAATCGAGCTGAAAGACGCTTTCGAAAACATGGGCGCCCAGCTGGTCAAGGAAGTTGCTTCCAAGGCCAACGACGCTGCCgg                                                                                                                                   >  2:3414326/1‑150 (MQ=255)

                       tGGTACTGGCCAAGAGCTTCGGCGCGCCGACCATCACCAAGGACGGCGTTTCCGTCGCCAAAGAAATCGAGCTGAAAGACGCTTTCGAAAACATGGGCGCCCAGCTGGTCAAGGAAGTTGCTTCCAAGGCCAACGACGCTGCCGGTGACg                                                                                                                              >  2:1232408/1‑150 (MQ=255)

                        ggTACTGGCCAAGAGCTTCGGCGCGCCGACCATCACCAAGGACGGCGTTTCCGTCGCCAAAGAAATCGAGCTGAAAGACGCTTTCGAAAACATGGGCGCCCAGCTGGTCAAGGAAGTTGCTTCCAAGGCCAACGACGCTGCCGGTGACgg                                                                                                                             <  2:4203639/150‑1 (MQ=255)

                         gTACTGGCCAAGAGCTTCGGCGCGCCGACCATCACCAAGGACGGCGTTTCCGTCGCCAAAGAAATCGAGCTGAAAGACGCTTTCGAAAACATGGGCGCCCAGCTGGTCAAGGAAGTTGCTTCCAAGGCCAACGACGCTGCCGGTGACGGc                                                                                                                            <  1:3403398/150‑1 (MQ=255)

                            cTGGCCAAGAGCTTCGGCGCGCCGACCATCACCAAGGACGGCGTTTCCGTCGCCAAAGAAATCGAGCTGAAAGACGCTTTCGAAAACATGGGCGCCCAGCTGGTCAAGGAAGTTGCTTCCAAGGCCAACGACGCTGCCGGTGACGGcacc                                                                                                                         <  2:4987752/150‑1 (MQ=255)

                                ccAAGAGCTTCGGCGCGCCGACCATCACCAAGGACGGCGTTTCCGTCGCCAAAGAAATCGAGCTGAAAGACGCTTTCGAAAACATGGGCGCCCAGCTGGTCAAGGAAGTTGCTTCCAAGGCCAACGACGCTGCCGGTGACGGcaccacca                                                                                                                     >  1:2302873/1‑150 (MQ=255)

                                  aaGAGCTTCGGCGCGCCGACCATCACCAAGGACGGCGTTTCCGTCGCCAAAGAAATCGAGCTGAAAGACGCTTTCGAAAACATGGGCGCCCAGCTGGTCAAGGAAGTTGCTTCCAAGGCCAACGACGCTGCCGGTGACGGCaccaccacc                                                                                                                   >  2:2883467/1‑150 (MQ=255)

                                      gCTTCGGCGCGCCGACCATCACCAAGGACGGCGTTTCCGTCGCCAAAGAAATCGAGCTGAAAGACGCTTTCGAAAACATGGGCGCCCAGCTGGTCAAGGAAGTTGCTTCCAAGGCCAACGACGCTGCCGGTGACGGCACCACCACCGCTa                                                                                                               <  1:1437285/150‑1 (MQ=255)

                                      ccTTCGGCGCTCCACGCATCACCAAGGACGGTGTATCGGTTGCCAAGGAAATCGAACTGGAAGACAAGTTCGAAAACATGGGCGCACAGATGCTGCGCGAAGTGGCTTCGAAGACCAACGACACTGCCGGTGACGGCACCACCACTGcga                                                                                                               >  1:4034462/2‑148 (MQ=255)

                                       cTTCGGCGCGCCGACCATCACCAAGGACGGCGTTTCCGTCGCCAAAGAAATCGAGCTGAAAGACGCTTTCGAAAACATGGGCGCCCAGCTGGTCAAGGAAGTTGCTTCCAAGGCCAACGACGCTGCCGGTGACGGCACCACCACCGCTAc                                                                                                              >  1:2170819/1‑150 (MQ=255)

                                        ttCGGCGCTCCACGCATCACCAAGGACGGTGTATCGGTTGCCAAGGAAATCGAACTGGAAGACAAGTTCGAAAACATGGGCGCACAGATGCTGCGCGAAGTGGCTTCGAAGACCAACGACACTGCCGGTGACGGCACCACCACTGCGAcc                                                                                                             >  2:5278307/1‑150 (MQ=255)

                                        ttCGGCGCTCCACGCATCACCAAGGACGGTGTATCGGTTGCCAAGGAAATCGAACTGGAAGACAAGTTCGAAAACATGGGCGCACAGATGCTGCGCGAAGTGGCTTCGAAGACCAACGACACTGCCGGTGACGGCACCACCACTGCGAcc                                                                                                             >  2:5341984/1‑150 (MQ=255)

                                        ttCGGCGCGCCGACCATCACCAAGGACGGCGTTTCCGTCGCCAAAGAAATCGAGCTGAAAGACGCTTTCGAAAACATGGGCGCCCAGCTGGTCAAGGAAGTTGCTTCCAAGGCCAACGACGCTGCCGGTGACGGCACCACCACCGCTAcc                                                                                                             >  1:1352239/1‑150 (MQ=255)

                                        ttCGGCGCGCCGACCATCACCAAGGACGGCGTTTCCGTCGCCAAAGAAATCGAGCTGAAAGACGCTTTCGAAAACATGGGCGCCCAGCTGGTCAAGGAAGTTGCTTCCAAGGCCAACGACGCTGCCGGTGACGGCACCACCACCGCTAcc                                                                                                             >  2:1416183/1‑150 (MQ=255)

                                            gcgcgcCGACCATCACCAAGGACGGCGTTTCCGTCGCCAAAGAAATCGAGCTGAAAGACGCTTTCGAAAACATGGGCGCCCAGCTGGTCAAGGAAGTTGCTTCCAAGGCCAACGACGCTGCCGGTGACGGCACCACCACCGCTACCGTTc                                                                                                         >  1:4934728/1‑150 (MQ=255)

                                            gcgcgcCGACCATCACCAAGGACGGCGTTTCCGTCGCCAAAGAAATCGAGCTGAAAGACGCTTTCGAAAACATGGGCGCCCAGCTGGTCAAGGAAGTTGCTTCCAAGGCCAACGACGCTGCCGGTGACGGCACCACCACCGCTACCGTTc                                                                                                         <  2:2231213/150‑1 (MQ=255)

                                             cgcgcCGACCATCACCAAGGACGGCGTTTCCGTCGCCGAAGAAATCGAGCTGAAAGACGCTTTCGAAAACATGGGCGCCCAGCTGGTCAAGGAAGTTGCTTCCAAGGCCAACGACGCTGCCGGTGACGGCACCACCACCGCTACCGTTCt                                                                                                        <  2:4049156/150‑1 (MQ=255)

                                             cgcgcCGACCATCACCAAGGACGGCGTTTCCGTCGCCAAAGAAATCGAGCTGAAAGACGCTTTCGAAAACATGGGCGCCCAGCTGGTCAAGGAAGTTGCTTCCAAGGCCAACGACGCTGCCGGTGACGGCACCACCACCGCTACCGTTCt                                                                                                        <  1:3607078/150‑1 (MQ=255)

                                             cgcgcCGACCATCACCAAGGACGGCGTTTCCGTCGCCAAAGAAATCGAGCTGAAAGACGCTTTCGAAAACATGGGCGCCCAGCTGGTCAAGGAAGTTGCTTCCAAGGCCAACGACGCTGCCGGTGACGGCACCACCACCGCTACCGTTCt                                                                                                        <  2:51194/150‑1 (MQ=255)

                                             cgcgcCGACCATCACCAAGGACGGCGTTTCCGTCGCCAAAGAAATCGAGCTGAAAGACGCTTTCGAAAACATGGGCGCCCAGCTGGTCAAGGAAGTTGCTTCCAAGGCCAACGACGCTGCCGGTGACGGCACCACCACCGCTACCGTTCt                                                                                                        <  1:414080/150‑1 (MQ=255)

                                              gcgcCGACCATCACCAAGGACGGCGTTTCCGTCGCCAAAGAAATCGAGCTGAAAGACGCTTTCGAAAACATGGGCGCCCAGCTGGTCAAGGAAGTTGCTTCCAAGGCCAACGACGCTGCCGGTGACGGCACCACCACCGCTACCGTTCTg                                                                                                       >  2:1002034/1‑150 (MQ=255)

                                               cgcCGACCCTCACCAAGGACGGCGTTTCCGTCGCCAAAGAACGCGCGCTGAAAGACGCTTTCGAAAACATGGGCGCCCAGCTGGTCAAGGAAGTTGCTTCCAAGGCCAACGACGCTGCCGGTGACGGCACCACCACCGCTACCGTTCTgg                                                                                                      <  2:2800328/150‑1 (MQ=255)

                                                   gACCATCACCAAGGACGGCGTTTCCGTCGCCAAAGAAATCGAGCTGAAAGACGCTTTCGAAAACATGGGCGCCCAGCTGGTCAAGGAAGTTGCTTCCAAGGCCAACGACGCTGCCGGTGACGGCACCACCACCGCTACCGTTCTGGCTCa                                                                                                  <  1:1867578/150‑1 (MQ=255)

                                                    aCCATCACCAAGGACGGCGTTTCCGTCGCCAAAGAAATCGAGCTGAAAGACGCTTTCGAAAACATGGGCGCCCAGCTGGTCAAGGAAGTTGCTTCCAAGGCCAACGACGCTGCCGGTGACGGCACCACCACCGCTACCGTTCTGGCTCAg                                                                                                 <  2:4189309/150‑1 (MQ=255)

                                                    aCCATCACCAAGGACGGCGTTTCCGTCGCCAAAGAAATCGAGCTGAAAGACGCTTTCGAAAACATGGGCGCCCAGCTGGTCAAGGAAGTTGCTTCCAAGGCCAACGACGCTGCCGGTGACGGCACCACCACCGCTACCGTTCTGGCTCAg                                                                                                 <  1:5339013/150‑1 (MQ=255)

                                                     gcATCACCAAGGACGGTGTATCGGTTGCCAAGGAAATCGAACTGGAAGACAAGTTCGAAAACATGGGCGCACAGATGCTGCGCGAAGTGGCTTCGAAGACCAACGACACTGCCGGTGACGGCACCACCACTGCGACCGTTCTCGGTCAgg                                                                                                >  1:4828461/2‑150 (MQ=255)

                                                        tCACCAAGGACGGCGTTTCCGTCGCCAAAGAAATCGAGCTGAAAGACGCTTTCGAAAACATGGGCGCCCAGCTGGTCAAGGAAGTTGCTTCCAAGGCCAACGACGCTGCCGGTGACGGCACCACCACCGCTACCGTTCTGGCTCAGGCCa                                                                                             >  2:1707257/1‑150 (MQ=255)

                                                          aCCAAGGACGGTGTATCGGTTGCCAAGGAAATCGAACTGGAAGACAAGTTCGAAAACATGGGCGCACAGATGCTGCGCGAAGTGGCTTCGAAGACCAACGACACTGCCGGTGACGGCACCACCACTGCGACCGTTCTCGGTCAGGCTATc                                                                                           <  1:3305332/150‑1 (MQ=255)

                                                          aCCAAGGACGGCGTTTCCGTCGCCAAAGAAATCGAGCTGAAAGACGCTTTCGAAAACATGGGCGCCCAGCTGGTCAAGGAAGTTGCTTCCAAGGCCAACGACGCTGCCGGTGACGGCACCACCACCGCTACCGTTCTGGCTCAGGCCATc                                                                                           <  1:1967786/150‑1 (MQ=255)

                                                          aCCAAGGACGGCGTTTCCGTCGCCAAAGAAATCGAGCTGAAAGACGCTTTCGAAAACATGGGCGCCCAGCTGGTCAAGGAAGTTGCTTCCAAGGCCAACGACGCTGCCGGTGACGGCACCACCACCGCTACCGTTCTGGCTCAGGCCATc                                                                                           >  1:549646/1‑150 (MQ=255)

                                                             aaGGACGGCGTTTCCGTCGCCAAAGAAATCGAGCTGAAAGACGCTTTCGAAAACATGGGCGCCCAGCTGGTCAAGGAAGTTGCTTCCAAGGCCAACGACGCTGCCGGTGACGGCACCACCACCGCTACCGTTCTGGCTCAGGCCATCGTc                                                                                        >  1:4073065/1‑150 (MQ=255)

                                                              aGGACGGCGTTTCCGTCGCCAAAGAAATCGAGCTGAAAGACGCTTTCGAAAACATGGGCGCCCAGCTGGTCAAGGAAGTTGCTTCCAAGGCCAACGACGCTGCCGGTGACGGCACCACCACCGCTACCGTTCTGGCTCAGGCCATCGTCa                                                                                       <  2:1730879/150‑1 (MQ=255)

                                                               ggACGGTGTATCGGTTGCCAAGGAAATCGAACTGGAAGACAAGTTCGAAAACATGGGCGCACAGATGCTGCGCGAAGTGGCTTCGAAGACCAACGACACTGCCGGTGACGGCACCACCACTGCGACCGTTCTCGGTCAGGCTATCGttca                                                                                      <  2:1493699/150‑4 (MQ=255)

                                                                gACGGTGTATCGGTTGCCAAGGAAATCGAACTGGAAGACAAGTTCGAAAACATGGGCGCACAGATGCTGCGCGAAGTGGCTTCGAAGACCAACGACACTGCCGGTGACGGCACCACCACTGCGACCGTTCTCGGTCAGGCTATCGttcag                                                                                     >  2:4901322/1‑146 (MQ=255)

                                                                   ggCGTTTCCGTCGCCAAAGAAATCGAGCTGAAAGACGCTTTCGAAAACATGGGCGCCCAGCTGGTCAAGGAAGTTGCTTCCAAGGCCAACGACGCTGCCGGTGACGGCACCACCACCGCTACCGTTCTGGCTCAGGCCATCGTCAACGaa                                                                                  >  1:1465819/1‑150 (MQ=255)

                                                                         tCCGTCGCCAAAGAAATCGAGCTGAAAGACGCTTTCGAACACATGGGCGCCCAGCTGGTCAAGGAAGTTGCTTCCAAGGCCAACGACGCTGCCGGTGACGGCACCACCACCGCTACCGTTCTGGCTCAGGCCATCGTCAACGAAGGCCTg                                                                            <  2:842396/150‑1 (MQ=255)

                                                                            gTCGCCAAAGAAATCGAGCTGAAAGACGCTTTCGAAAACATGGGCGCCCAGCTGGTCAAGGAAGTTGCTTCCAAGGCCAACGACGCTGCCGGTGACGGCACCACCACCGCTACCGTTCTGGCTCAGGCCATCGTCAACGAAGGCCTGaaa                                                                         <  1:2074508/150‑1 (MQ=255)

                                                                            gTCGCCAAAGAAATCGAGCTGAAAGACGCTTTCGAAAACATGGGCGCCCAGCTGGTCAAGGAAGTTGCTTCCAAGGCCAACGACGCTGCCGGTGACGGCACCACCACCGCTACCGTTCTGGCTCAGGCCATCGTCAACGAAGGCCTGaaa                                                                         <  1:4020978/150‑1 (MQ=255)

                                                                               gCCAAAGAAATCGAGCTGAAAGACGCTTTCGAAAACATGGGCGCCCAGCTGGTCAAGGAAGTTGCTTCCAAGGCCAACGACGCTGCCGGTGACGGCACAACCACCGCTACCGTTCTGGCTCAGGCCATCGTCAACGAAGGCCTGAAAGcc                                                                      >  1:2219153/1‑150 (MQ=255)

                                                                                cctaaGAATTCGAGCTGAAAGTCGCTTTCGAATACCTGGGCGCCCAGCTGGTCTAGGATGTTGCTTCCAAGGCCAACGACGGTGCCGGTGACGGCAGCACCACCGCTACCGTTCTGGCTCAGGCCATCGTCAACGAAGGCCTGAAAGCCg                                                                     <  2:2002704/147‑1 (MQ=255)

                                                                                 cAAAGAAATCGAGCTGAAAGACGCTTTCGAAAACATGGGCGCCCAGCTGGTCAAGGAAGTTGCTTCCAAGGCCAACGACGCTGCCGGTGACGGCGCCACCACCGCTACCGTTCTGGCTCAGGCCATCGTCAACGAAGGCCTGAAAGCCGt                                                                    >  2:4522324/1‑150 (MQ=255)

                                                                                 cAAAGAAATCGAGCTGAAAGACGCTTTCGAAAACATGGGCGCCCAGCTGGTCAAGGAAGTTGCTTCCAAGGCCAACGACGCTGCCGGTGACGGCACCACCACCGCTACCGTTCTGGCTCAGGCCATCGTCAACGAAGGCCTGAAAGCCGt                                                                    >  1:4284876/1‑150 (MQ=255)

                                                                                  aaaGAAATCGAGCTGAAAGACGCTTTCGAAAACATGGGCGCCCAGCTGGTCAAGGAAGTTGCTTCCAAGGCCAACGACGCTGCCGGTGACGGCACCACCACCGCTACCGTTCTGGCTCAGGCCATCGTCAACGAAGGCCTGAAAGCCGTc                                                                   >  1:2345739/1‑150 (MQ=255)

                                                                                            aGCTGAAAGACGCTTTCGAAAACATGGGCGCCCATCTGGTCAAGGAAGTTGCTTCCAAGGCCAACGACGCTGCCGTTGACGGCACCACCACCGCTACCGTTCTGGCTCAGGCAATCGTCAACGAAGGCCTGAAAGCCGTCGCTGCCGGCa                                                         <  1:4672526/150‑1 (MQ=255)

                                                                                             gCTGAAAGACGCTTTCGAAAACATGGGCGCCCAGCTGGTCAAGGAAGTTGCTTCCAAGGCCAACGACGCTGCCGGTGACGGCACCACCACCGCTACCGTTCTGGCTCAGGCCATCGTCAACGAAGGCCTGAAAGCCGTCGCTGCCGGCAt                                                        <  2:2931253/150‑1 (MQ=255)

                                                                                                    gACGCTTTCGAAAACATGGGCGCCCAGCTGGTCAAGGAAGTTGCTTCCAAGGCCAACGACGCTGCCGGTGACGGCACCACCACCGCTACCGTTCTGGCTCAGGCCATCGTCAACGAAGGCCTGAAAGCCGTCGCTGCCGGCATGAACCCg                                                 >  2:3768277/1‑150 (MQ=255)

                                                                                                     acaagttCGAAAACATGGGCGCACAGATGCTGCGCGAAGTGGCTTCGAAGACCAACGACACTGCCGGTGACGGCACCACCACTGCGACCGTTCTCGGTCAGGCTATCGTTCAGGAAGGCGCCAAGGCTGTTGCCGCTGGCATGAAcccaa                                                >  1:4172471/6‑148 (MQ=255)

                                                                                                     aCGCTTTCGAAAACATGGGCGCCCAGCTGGTCAAGGAAGTTGCTTCCAAGGCCAACGACGCTGCCGGTGACGGCACCACCACCGCTACCGTTCTGGCTCAGGCCATCGTCAACGAAGGCCTGAAAGCCGTCGCTGCCGGCATGAACCCGa                                                >  2:782035/1‑150 (MQ=255)

                                                                                                     aCGCTTTCGAAAACATGGGCGCCCAGCTGGTCAAGGAAGTTGCTCCCAAGGCCAACGACGCTGCCGGTGACGGCACCACCACCGCTACCGTTCTGGCTCAGGCCATCGTCAACGAAGGCCTGAAAGCCGTCGCTGCCGGCATGAACCCGa                                                >  1:3587997/1‑150 (MQ=255)

                                                                                                       gCTTTCGAAAACATGGGCGCCCAGCTGGTCAAGGAAGTTGCTTCCAAGGCCAACGACGCTGCCGGTGACGGCACCACCACCGCTACCGTTCTGGCTCAGGCCATCGTCAACGAAGGCCTGAAAGCCGTCGCTGCCGGCATGAACCCGATg                                              >  2:3197772/1‑150 (MQ=255)

                                                                                                       gCTTTCGAAAACATGGGCGCCCAGCTGGTCAAGGAAGTTGCTTCCAAGGCCAACGACGCTGCCGGTGACGGCACCACCACCGCTACCGTTCTGGCTCAGGCCATCGTCAACGAAGGCCTGAAAGCCGTCGCTGCCGGCATGAACCCGATg                                              <  1:782035/150‑1 (MQ=255)

                                                                                                       gCTTTCGAAAACATGGGCGCCCAGCTGGTCAAGGAAGTTGCTTCCAAGGCCAACGACGCTGCCGGTGACGGCACCACCACCGCTACCGTTCTGGCTCAGGCCATCGTCAACGAAGGCCTGAAAGCCGTCGCTGCCGGCATGAACCCGATg                                              <  2:2341306/150‑1 (MQ=255)

                                                                                                       gCTTTCGAAAACATGGGCGCCCAGCTGGTCAAGGAAGTTGCTTCCAAGGCCAACGACGCTGCCGGTGACGGCACCACCACCGCTACCGTTCTGGCTCAGGCCATCGTCAACGAAGGCCTGAAAGCCGTCGCTGCCGGCATGAACCCGATg                                              >  2:1853486/1‑150 (MQ=255)

                                                                                                       gCTTTCGAAAACATGGGCGCCCAGCTGGTCAAGGAAGTTGCTTCCAAGGCCAACGACGCTGCCGGTGACGGCACCACCACCGCTACCGTTCTGGCTCAGGCCATCGTCAACGAAGGCCTGAAAGCCGTCGCTGCCGGCATGAACCCGATg                                              <  1:2744422/150‑1 (MQ=255)

                                                                                                       aagttCGAAAACATGGGCGCACAGATGCTGCGCGAAGTGGCTTCGAAGACCAACGACACTGCCGGTGACGGCACCACCACTGCGACCGTTCTCGGTCAGGCTATCGTTCAGGAAGGCGCCAAGGCTGTTGCCGCTGGCATGAACCCAATg                                              >  2:465880/4‑150 (MQ=255)

                                                                                                         tttCGAAAACATGGGCGCCCAGCTGGTCAAGGAAGTTGCTTCCAAGGCCAACGACGCTGCCGGTGACGGCACCACCACCGCTACCGTTCTGGCTCAGGCCATCGTCAACGAAGGCCTGAAAGCCGTCGCTGCCGGCATGAACCCGATGGa                                            >  2:4102516/1‑150 (MQ=255)

                                                                                                          ttCGAAAACATGGGCGCCCAGCTGGTCAAGGAAGTTGCTTCCAAGGCCAACGACGCTGCCGGTGACGGCACCACCACCGCTACCGTTCTGGCTCAGGCCATCGTCAACGAAGGCCTGAAAGCCGTCGCTGCCGGCATGAACCCGATGGAc                                           <  2:2131630/150‑1 (MQ=255)

                                                                                                          ttCGAAAACATGGGCGCCCAGCTGGTCAAGGAAGTTGCTTCCAAGGCCAACGACGCTGCCGGTGACGGCACCACCACCGCTACCGTTCTGGCTCAGGCCATCGTCAACGAAGGCCTGAAAGCCGTCGCTGCCGGCATGAACCCGATGGAc                                           >  2:3452918/1‑150 (MQ=255)

                                                                                                          ttCGAAAACATGGGCGCACAGATGCTGCGCGAAGTGGCTTCGAAGACCAACGACACTGCCGGTGACGGCACCACCACTGCGACCGTTCTCGGTCAGGCTATCGTTCAGGAAGGCGCCAAGGCTGTTGCCGCTGGCATGaa                                                     >  1:1174108/1‑140 (MQ=255)

                                                                                                          ttCGAAAACATGGGCGCACAGATGCTGCGCGAAGTGGCTTCGAAGACCAACGACACTGCCGGTGACGGCACCACCACTGCGACCGTTCTCGGTCAGGCTATCGTTCAGGAAGGCGCCAAGGCTGTTGCCGCTGGCATGaa                                                     <  2:1174108/140‑1 (MQ=255)

                                                                                                          ttCGAAAACATGGGCGCACAGATGCTGCGCGAAGTGGCTTCGAAGACCAACGACACTGCCGGTGACGGCACCACCACTGCGACCGTTCTCGGTCAGGCTATCGTTCAGGAAGGCGCCAAGGCTGTTGCCGCTGGCATGAACCCAATGGAc                                           >  1:2847167/1‑150 (MQ=255)

                                                                                                            cGAAAACATGGGCGCACAGATGCTGCGCGAAGTGGCTTCGAAGACCAACGACACTGCCGGTGACGGCACCACCACTGCGACCGTTCTCGGTCAGGCTATCGTTCAGGAAGGCGCCAAGGCTGTTGCCGCTGGCATGAACCCAATGGACCt                                         >  1:539582/1‑150 (MQ=255)

                                                                                                             gAAAACATGGGCGCCCAGCTGGTCAAGGAAGTTGCTTCCAAGGCCAACGACGCTGCCGGTGACGGCACCACCACCGCTACCGTTCTGGCTCAGGCCATCGTCAACGAAGGCCTGAAAGCCGTCGCTGCCGGCATGAACCCGATGGACCTg                                        >  1:1246359/1‑150 (MQ=255)

                                                                                                               aaaCATGGGCGCCCAGCTGGTCAAGGAAGTTGCTTCCAAGGCCAACGACGCTGCCGGTGACGGCACCACCACCGCTACCGTTCTGGCTCAGGCCATCGTCAACGAAGGCCTGAAAGCCGTCGCTGCCGGCATGAACCCGATGGACCTGaa                                      <  1:3451200/150‑1 (MQ=255)

                                                                                                                   aTGGGCGCCCAGCTGGTCAAGGACGGTGCTTCCAAGGCCAACGACGCTGCCGGTGACGGCACCACCACCGCTACCGTTCTGGCTCAGGCCATCGTCAACGAAGGCCTGAAAGCCGTCGCTGCCGGCATGAACCCGATGGACCTGAAgcgc                                  <  2:4558128/150‑1 (MQ=255)

                                                                                                                   aTGGGCGCCCAGCTGGTCAAGGAAGTTGCTTCCAAGGCCAACGACGCTGCCGGTGACGGCACCACCACCGCTACCGTTCTGGCTCAGGCCATCGTCAACGAAGGCCTGAAAGCCGTCGCTGCCGGCATGAACCCGATGGACCTGAAgcgc                                  >  1:3873803/1‑150 (MQ=255)

                                                                                                                    tGGGCGCCCAGCTGGTCAAGGAAGTTGCTTCCAAGGCCAACGACGCTGCCGGTGACGGCACCACCACCGCTACCGTTCTGGCTCAGGCCATCGTCAACGAAGGCCTGAAAGCCGTCGCTGCCGGCATGAACCCGATGGACCTGAAGcgcg                                 <  2:3971086/150‑1 (MQ=255)

                                                                                                                     gggCGCCCAGCTGGTCAAGGAAGTTGCTTCCAAGGCCAACGACGCTGCCGGTGACGGCACCACCACCGCTACCGTTCTGGCTCAGGCCATCGTCAACGAAGGCCTGAAAGCCGTCGCTGc                                                              >  1:4456802/1‑120 (MQ=255)

                                                                                                                     gggCGCCCAGCTGGTCAAGGAAGTTGCTTCCAAGGCCAACGACGCTGCCGGTGACGGCACCACCACCGCTACCGTTCTGGCTCAGGCCATCGTCAACGAAGGCCTGAAAGCCGTCGCTGCCGGCATGAACCCGATGGACCTGAAGCGCgg                                <  2:2750421/150‑1 (MQ=255)

                                                                                                                         gcCCAGCTGGTCAAGGAAGTTGCTTCCAAGGCCAACGACGCTGCCGGTGACGGCACCACCACCGCTACCGTTCTGGCTCAGGCCATCGTCAACGAAGGCCTGAAAGCCGTCGCTGCCGGCATGAACCCGATGGACCTGAAGCGCGGTATc                            >  1:1522263/1‑150 (MQ=255)

                                                                                                                         gcCCAGCTGGTCAAGGAAGTTGCTTCCAAGGCCAACGACGCTGCCGGTGACGGCACCACCACCGCTACCGTTCTGGCTCAGGCCATCGTCAACGAAGGCCTGAAAGCCGTCGCTGCCGGCATGAACCCGATGGACCTGAAGCGCGGTATc                            <  1:3953869/150‑1 (MQ=255)

                                                                                                                                tGGTCAAGGAAGTTGCTTCCAAGGCCAACGACGCTGCCGGTGACGGCACCACCACCGCTACCGTTCTGGCTCAGGCCATCGTCAACGAAGGCCTGAAAGCCGTCGCTGCCGGCATGAACCCGATGGACCTGAAGCGCGGTATCGACAAgg                     <  1:2863731/150‑1 (MQ=255)

                                                                                                                                 ggTCAAGGAAGTTGCTTCCAAGGCCAACGACGCTGCCGGTGACGGCACCACCACCGCTACCGTTCTGGCTCAGGCCATCGTCAACGAAGGCCTGAAAGCCGTCGCTGCCGGCATGAACCCGATGGACCTGAAGCGCGGTATCGACAAGGc                    <  2:549646/150‑1 (MQ=255)

                                                                                                                                  gTCAAGGAAGTTGCTTCCAAGGCCAACGACGCTGCCGGTGACGGCACCACCACCGCTACCGTTCTGGCTCAGGCCATCGTCAACGAAGGCCTGAAAGCCGTCGCTGCCGGCATGAACCCGATGGACCTGAAGCGCGGTATCGACAAGGcc                   >  1:266473/1‑150 (MQ=255)

                                                                                                                                   tCAAGGAAGTTGCTTCCAAGGCCAACGACGCTGCCGGTGACGGCACCACCACCGCTACCGTTCTGGCTCAGGCCATCGTCAACGAAGGCCTGAAAGCCGTCGCTGCCGGCa                                                         <  2:4493767/111‑1 (MQ=255)

                                                                                                                                   tCAAGGAAGTTGCTTCCAAGGCCAACGACGCTGCCGGTGACGGCACCACCACCGCTACCGTTCTGGCTCAGGCCATCGTCAACGAAGGCCTGAAAGCCGTCGCTGCCGGCa                                                         >  1:4493767/1‑111 (MQ=255)

                                                                                                                                     aaGGAAGTTGCTTCCAAGGCCAACGACGCTGCCGGTGACGGCACCACCACCGCTACCGTTCTGGCTCAGGCCATCGTCAACGAAGGCCTGAAAGCCGTCGCTGCCGGCATGAACCCGATGGACCTGAAGCGCGGTATCGACAAGGCCAct                <  2:5036373/150‑1 (MQ=255)

                                                                                                                                      gcgAAGTGGCTTCGAAGACCAACGACACTGCCGGTGACGGCACCACCACTGCGACCGTTCtc                                                                                                       >  1:3023006/3‑61 (MQ=255)

                                                                                                                                      gcgAAGTGGCTTCGAAGACCAACGACACTGCCGGTGACGGCACCACCACTGCGACCGTTCtc                                                                                                       <  2:3023006/60‑2 (MQ=255)

                                                                                                                                      aGGAAGTTGCTTCCAAGGCCAACGACGCTGCCGGTGACGGCACCACCACCGCTACCGTTCTGGCTCAGGCCATCGTCAACGAAGGCCTGAAAGCCGTCGCTGCCGGCATGAACCCGATGGACCTGAAGCGCGGTATCGACAAGGCCActg               >  1:4341707/1‑150 (MQ=255)

                                                                                                                                       ggAAGTTGCTTCCAAGGCCAACGACGCTGCCGGTGACGGCACCACCACCGCTACCGTTCTGGCTCAGGCCATCGTCAACGAAGGCCTGAAAGCCGTCGCTGCCGGCATGAACCCGATGGACCTGAAGCGCGGTATCGACAAGGCCActgc              >  1:3286465/1‑150 (MQ=255)

                                                                                                                                        gAAGTTGCTTCCAAGGCCAACGACGCTGCCGGTGACGGCACCACCACCGCTACCGTTCTGGCTCAGGCCATCGTCAACGAAGGCCTGAAAGCCGTCGCTGCCGGCATGAACCCGATGGACCTGAAGCGCGGTATCGACAAGGCCActgct             >  1:889024/1‑150 (MQ=255)

                                                                                                                                               cTTCCAAGGCCAACGACGCTGCCGGTGACGGCACCACCACCGCTACCGTTCTGGCTCAGGCCATCGt                                                                                         <  1:1729751/67‑1 (MQ=255)

                                                                                                                                                ttCCAAGGCCAACGACGCTGCCGGTGACGGCACCACCACCGCTACCGTTCTGGCTCAGGCCATCGTCAACGAAGGCCTGAAAGCCGTCGCTGCCGGCATGAACCCGATGGACCTGAAGCGCGGTATCGACAAGGCCACTGCTGCcgtcgt     >  1:2592348/1‑150 (MQ=255)

                                                                                                                                                 tCCAAGGCCAACGACGCTGCCGGTGACGGCACCACCACCGCTACCGTTCTGGCTCAGGCCATCGTCAACGAAGGCCTGAAAGCCGTCGCTGCCGGCATGAACCCGATGGACCTGAAGCGCGGTATCGACAAGGCCACTGCTGCCGTCGtt    >  2:1955200/1‑150 (MQ=255)

                                                                                                                                                  ccAAGGCCAACGACGCTGCCGGTGACGGCACCACCACCGCTACCGTTCTGGCTCAGGCCATCGTCAACGAAGGCCTGAAAGCCGTCGCTGCCGGCATGAACCCGATGGACCTGAAGCGCGGTATCGACAAGGCCAc                 <  2:4857979/136‑1 (MQ=255)

                                                                                                                                                  ccAAGGCCAACGACGCTGCCGGTGACGGCACCACCACCGCTACCGTTCTGGCTCAGGCCATCGTCAACGAAGGCCTGAAAGCCGTCGCTGCCGGCATGAACCCGATGGACCTGAAGCGCGGTATCGACAAGGCCACTGCTGCCGTCGTTg   >  1:936506/1‑150 (MQ=255)

CCTGGGCCCGAAAGGCCGTAACGTGGTACTGGCCAAGAGCTTCGGCGCGCCGACCATCACCAAGGACGGCGTTTCCGTCGCCAAAGAAATCGAGCTGAAAGACGCTTTCGAAAACATGGGCGCCCAGCTGGTCAAGGAAGTTGCTTCCAAGGCCAACGACGCTGCCGGTGACGGCACCACCACCGCTACCGTTCTGGCTCAGGCCATCGTCAACGAAGGCCTGAAAGCCGTCGCTGCCGGCATGAACCCGATGGACCTGAAGCGCGGTATCGACAAGGCCACTGCTGCCGTCGTTGC  >  NC_002947/1549732‑1550028

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`