BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	1,550,212	A→T	5.0%	V190V (GTA→GTT)	groL →	chaperonin GroEL

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	1,550,212	0	A	T	5.0%	539.3 / 12.0	159	V190V (GTA→GTT)	groL	chaperonin GroEL
Reads supporting (aligned to +/- strand): ref base A (67/83); new base T (5/3); total (73/86)
Fisher's exact test for biased strand distribution p-value = 4.70e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.41e-01

Only 100 of 164 total aligned reads displayed.

TCCAAGGCCATCGCCCAGGTAGGTACCATCTCTGCCAACTCCGACAACTCCATCGGTGAAATCATCGCCGAAGCCATGGAAAAAGTCGGTAAAGAAGGCGTGATCACCGTTGAAGAAGGCTCGGGCCTGGAAAACGAACTGTCCGTCGTAGAAGGCATGCAGTTCGACCGCGGCTACCTGTCGCCGTACTTCGTCAACAAGCCGGACACCATGGTTGCCGAGCTGGAAGGCCCGCTGCTGCTGCTGGTCGACAAGAAAATCTCCAACATCCGTGAGCTGCTGCCAGTTCTGGAAGCCGT  >  NC_002947/1550063‑1550361

tCCAAGGCCATCGCCCAGGTAGGTACCATCTCTGCCAACTCCGACAACTCCATCGGTGAAATCATCGCCGAAGCCATGGAAAAAGTCGGTAAAGAAGGCGTGATCACCGTTGAAGAAGGCTCGGGCCTGGAAAACGTACTGTCCGTCGTa                                                                                                                                                       >  2:4569508/1‑150 (MQ=255)

tCCAAGGCCATCGCCCAGGTAGGTACCATCTCTGCCAACTCCGACAACTCCATCGGTGAAATCATCGCCGAAGCCATGGAAAAAGTCGGTAAAGAAGGCGTGATCACCGTTGAAGAAGGCTCGGGCCTGGAAAACGAACTGTCCGTCGTa                                                                                                                                                       >  2:5299720/1‑150 (MQ=255)

  cAAGGCCATCGCCCAGGTAGGTACCATCTCTGCCAACTCCGACAACTCCATCGGTGAAATCATCGCCGAAGCCATGGAAAAAGTCGGTAAAGAAGGCGTGATCACCGTTGAAGAAGGCTCGGGCCTGGAAAACGAACTGTCCGTCGTAGa                                                                                                                                                     >  2:837723/1‑150 (MQ=255)

    aGGCCATCGCCCAGGTAGGTACCATCTCTGCCAACTCCGACAACTCCATCGGTGAAATCATCGCCGAAGCCATGGAAAAAGTCGGTAAAGAAGGCGTGATCACCGTTGAAGAAGGCTCGGGCCTGGAAAACGAACTGTCCGTCGTAGAAg                                                                                                                                                   <  1:5299550/150‑1 (MQ=255)

    aGGCCATCGCCCAGGTAGGTACCATCTCTGCCAACTCCGACAACTCCATCGGTGAAATCATCGCCGAAGCCATGGAAAAAGTCGGTAAAGAAGGCGTGATCACCGTTGAAGAAGGCTCGGGCCTGGAAAACGAACTGTCCGTCGTAGAAg                                                                                                                                                   <  1:5299811/150‑1 (MQ=255)

        cATCGCCCAGGTAGGTACCATCTCTGCCAACTCCGACAACTCCATCGGTGAAATCATCGCCGAAGCCATGGAAAAAGTCGGTAAAGAAGGCGTGATCACCGTTGAAGAAGGCTCGGGCCTGGAAAACGAACTGTCCGTCGTAGAAGGCAt                                                                                                                                               <  2:4775834/150‑1 (MQ=255)

         aTCGCCCAGGTAGGTACCATCTCTGCCAACTCCGACAACTCCATCGGTGAAATCATCGCCGAAGCCATGGAAAAAGTCGGTAAAGAAGGCGTGATCACCGTTGAAGAAGGCTCGGGCCTGGAAAACGAACTGTCCGTCGTAGAAGGCATg                                                                                                                                              <  2:3342615/150‑1 (MQ=255)

         aTCGCCCAGGTAGGTACCATCTCTGCCAACTCCGACAACTCCATCGGTGAAATCATCGCCGAAGCCATGGAAAAAGTCGGTAAAGAAGGCGTGATCACCGTTGAAGAAGGCTCGGGCCTGGAAAACGAACTGTCCGTCGTAGAAGGCATg                                                                                                                                              <  2:3897575/150‑1 (MQ=255)

             cccAGGTAGGTACCATCTCTGCCAACTCCGACAACTCCATCGGTGAAATCATCGCCGAAGCCATGGAAAAAGTCGGTAAAGAAGGCGTGATCACCGTTGAAGAAGGCTCGGGCCTGGAAAACGAACTGTCCGTCGTAGAAGGCATGCAGt                                                                                                                                          >  1:3025222/1‑150 (MQ=255)

              ccAGGTAGGTACCATCTCTGCCAACTCCGACAACTCCATCGGTGAAATCATCGCCGAAGCCATGGAAAAAGTCGGTAAAGAAGGCGTGATCACCGTTGAAGAAGGCTCGGGCCTGGAAAACGAACTGTCCGTCGTAGAAGGCATGCAGtt                                                                                                                                         <  2:1527855/150‑1 (MQ=255)

                     ggtaCCATCTCTGCCAACTCCGACAACTCCATCGGTGAAATCATCGCCGAAGCCATGGAAAAAGTCGGTAAAGAAGGCGTGATCACCGTTGAAGAAGGCTCGGGCCTGGAAAACGAACTGTCCGTCGTAGaa                                                                                                                                                    <  2:3829909/132‑1 (MQ=255)

                     ggtaCCATCTCTGCCAACTCCGACAACTCCATCGGTGAAATCATCGCCGAAGCCATGGAAAAAGTCGGTAAAGAAGGCGTGATCACCGTTGAAGAAGGCTCGGGCCTGGAAAACGAACTGTCCGTCGTAGaa                                                                                                                                                    >  1:3829909/1‑132 (MQ=255)

                     ggtaCCATCTCTGCCAACTCCGACAACTCCATCGGTGAAATCATCGCCGAAGCCATGGAAAAAGTCGGTAAAGAAGGCGTGATCACCGTTGAAGAAGGCTCGGGCCTGGAAAACGAACTGTCCGTCGTAGaa                                                                                                                                                    >  2:5001540/1‑132 (MQ=255)

                     ggtaCCATCTCTGCCAACTCCGACAACTCCATCGGTGAAATCATCGCCGAAGCCATGGAAAAAGTCGGTAAAGAAGGCGTGATCACCGTTGAAGAAGGCTCGGGCCTGGAAAACGAACTGTCCGTCGTAGaa                                                                                                                                                    <  1:5001540/132‑1 (MQ=255)

                     gggaCCATCTCTGCCAACTCCGACAACTCCATCGGTGAAATCATCGCCGAAGCCATGGAAAAAGTCGGTAAAGAAGGCGTGATCACCGTTGAAGAAGGCTCGGGCCTGGAAAACGAACTGTCCGTCGTAGAAGGCATGCAGTTCGACcgc                                                                                                                                  <  1:3568002/147‑1 (MQ=255)

                       taCCATCTCTGCCAACTCCGACAACTCCATCGGTGAAATCATCGCCGAAGCCATGGAAAAAGTCGGTAAAGAAGGCGTGATCACCGTTGAAGAAGGCTCGGGCCTGGAAAACGAACTGTCCGTCGTAGAAGGCATGCAGTTCGACCGCgg                                                                                                                                <  2:347278/150‑1 (MQ=255)

                        aCCATCTCTGCCAACTCCGACAACTCCATCGGTGAAATCATCGCCGAAGCCATGGAAAAAGTCGGTAAAGAAGGCGTGATCACCGTTGAAGAAGGCTCGGGCCTGGAAAACGAACTGTCCGTCGTAGAAGGCATGCAGTTCGACCGCGGc                                                                                                                               >  2:4638923/1‑150 (MQ=255)

                        aCCATCTCTGCCAACTCCGACAACTCCATCGGTGAAATCATCGCCGAAGCCATGGAAAAAGTCGGTAAAGAAGGCGTGATCACCGTTGAAGAAGGCTCGGGCCTGGAAAACGAACTGTCCGTCGTAGAAGGCATGCAGTTCGACCGCGGc                                                                                                                               >  2:4638977/1‑150 (MQ=255)

                        aCCATCTCTGCCAACTCCGACAACTCCATCGGTGAAATCATCGCCGAAGCCATGGAAAAAGTCGGTAAAGAAGGCGTGATCACCGTTGAAGAAGGCTCGGGCCTGGAAAACGAACTGTCCGTCGTAGAAGGCATGCAGTTCGACCGCGGc                                                                                                                               >  2:2914554/1‑150 (MQ=255)

                          cATCTCTGCCAACTCCGACAACTCCATCGGTGAAATCATCGCCGAAGCCATGGAAAAAGTCGGTAAAGAAGGCGTGATCACCGTTGAAGAAGGCTCGGGCCTGGAAAACGAACTGTCCGTCGTAGAAGGCATGCAGTTCGACCGCGGCTa                                                                                                                             >  1:1483914/1‑150 (MQ=255)

                           aTCTCTGCCAACTCCGACAACTCCATCGGTGAAATCATCGCCGAAGCCATGGAAAAAGTCGGTAAAGAAGGCGTGATCACCGTTGAAGAAGGCTCGGGCCTGGAAAACGAACTGTCCGTCGTAGAAGGCATGTAGTTCGACCGCGGCTAc                                                                                                                            >  1:4194460/1‑150 (MQ=255)

                            tctcTGCCAACTCCGACAACTCCATCGGTGAAATCATCGCCGAAGCCATGGAAAAAGTCGGTAAAGAAGGCGTGATCACCGTTGAAGAAGGCTCGGGCCTGGAAAACGAACTGTCCGTCGTAGAAGGCATGCAGTTCGACCGCGGCTAcc                                                                                                                           >  1:1315697/1‑150 (MQ=255)

                                tGCCAACTCCGACAACTCCATCGGTGAAATCATCGCCGAAGCCATGGAAAAAGTCGGTAAAGAAGGCGTGATCACCGTTGAAGAAGGCTCGGGCCTGGAAAACGAACTGTCCGTCGTAGAAGGCATGCAGTTCGACcgcg                                                                                                                                 >  2:4100186/1‑140 (MQ=255)

                                tGCCAACTCCGACAACTCCATCGGTGAAATCATCGCCGAAGCCATGGAAAAAGTCGGTAAAGAAGGCGTGATCACCGTTGAAGAAGGCTCGGGCCTGGAAAACGAACTGTCCGTCGTAGAAGGCATGCAGTTCGACcgcg                                                                                                                                 <  1:4100186/140‑1 (MQ=255)

                                tGCCAACTCCGACAACTCCATCGGTGAAATCATCGCCGAAGCCATGGAAAAAGTCGGTAAAGAAGGCGTGATCACCGTTGAAGAAGGCTCGGGCCTGGAAAACGAACTGTCCGTCGTAGAAGGCATGCAGTTCGACCGCGGCTACCTGTc                                                                                                                       >  2:2454467/1‑150 (MQ=255)

                                 gCCAACTCCGACAACTCCATCGGTGAAATCATCGCCGAAGCCATGGAAAAAGTCGGTAAAGAAGGCGTGATCACCGTTGAAGAAGGCTCGGGCCTGGAAAACGAACTGTCCGTCGTAGAAGGCATGCAGTTCGACCGCGGCTACCTGTCg                                                                                                                      <  2:4143668/150‑1 (MQ=255)

                                 gCCAACTCCGACAACTCCATCGGTGAAATCATCGCCGAAGCCATGGAAAAAGTCGGTAAAGAAGGCGTGATCACCGTTGAAGAAGGCTCGGGCCTGGAAAACGAACTGTCCGTCGTAGAAGGCATGCAGTTCGACCGCGGCTACCTGTCg                                                                                                                      <  2:4101378/150‑1 (MQ=255)

                                   cAACTCCGACAACTCCATCGGTGAAATCATCGCCGAAGCCATGGAAAAAGTCGGTAAAGAAGGCGTGATCACCGTTGAAGAAGGCTCGGGCCTGGAAAACGAACTGTCCGTCGTAGAAGGCATGCAGTTCGACCGCGGCTACCTGTCGcc                                                                                                                    <  2:4186369/150‑1 (MQ=255)

                                   cAACTCCGACAACTCCATCGGTGAAATCATCGCCGAAGCCATGGAAAAAGTCGGTAAAGAAGGCGTGATCACCGTTGAAGAAGGCTCGGGCCTGGAAAACGAACTGTCCGTCGTAGAAGGCATGCAGTTCGACCGCGGCTACCTGTCGcc                                                                                                                    >  1:2512778/1‑150 (MQ=255)

                                   cAACTCCGACAACTCCATCGGTGAAATCATCGCCGAAGCCATGGAAAAAGTCGGTAAAGAAGGCGTGATCACCGTTGAAGAAGGCTCGGGCCTGGAAAACGAACTGTCCGTCGTAGAAGGCATGCAGTTCGACCGCGGCTACCTGTCGcc                                                                                                                    <  2:352090/150‑1 (MQ=255)

                                   cAACTCCGACAACTCCATCGGTGAAATCATCGCCGAAGCCATGGAAAAAGTCGGTAAAGAAGGCGTGATCACCGTTGAAGAAGGCTCGGGCCTGGAAAACGAACTGTCCGTCGTAGAAGGCATGCAGTTCGACCGCGGCTACCTGTCGcc                                                                                                                    >  1:2956017/1‑150 (MQ=255)

                                    aaCTCCGACAACTCCATCGGTGAAATCATCGCCGAAGCCATGGAAAAAGTCGGTAAAGAAGGCGTGATCACCGTTGAAGAAGGCTCGGGCCTGGAAAACGAACTGTCCGTCGTAGAAGGCATGCAGTTCGACCGCGGCTACCTGTCGCCg                                                                                                                   <  1:362882/150‑1 (MQ=255)

                                     aCTCCGACAACTCCATCGGTGAAATCATCGCCGAAGCCATGGAAAAAGTCGGTAAAGAAGGCGTGATCACCGTTGAAGAAGGCTCGGGCCTGGAAAACGAACTGTCCGTCGTAGAAGGCATGCAGTTCGACCGCGGCTACCTGTCGCCGt                                                                                                                  <  2:2919858/150‑1 (MQ=255)

                                      cTCCGACAACTCCATCGGTGAAATCATCGCCGAAGCCATGGAAAAAGTCGGTAAAGAAGGCGTGATCACCGTTGAAGAAGGCTCGGGCCTGGAAAACGAACTGTCCGTCGTAGAAGGCATGCAGTTCGACCGCGGCTACCTGTCGCCGTa                                                                                                                 <  1:3360372/150‑1 (MQ=255)

                                          gACAACTCCATCGGTGAAATCATCGCCGAAGCCATGGAAAAAGTCGGTAAAGAAGGCGTGATCACCGTTGAAGAAGGCTCGGGCCTGGAAAACGAACTGTCCGTCGTAGAAGGCATGCAGTTCGACCGCGGCTACCTGTCGCCGTACTTc                                                                                                             >  1:839711/1‑150 (MQ=255)

                                          gACAACTCCAGCGGTGAAATCATCGCCGAAGCCATGGAAAAAGTCGGTAAAGAAGGCGTGATCACCGTTGAAGAAGGCTCGGGCCTGGAAAACGAACTGTCCGTCGTAGAAGGCATGCAGTTCGACCGCGGCTACCTGTCGCCGTACTTc                                                                                                             <  2:226155/150‑1 (MQ=255)

                                             aaCTCCATCGGTGAAATCATCGCCGAAGCCATGGAAAAAGTCGGTAAAGAAGGCGTGATCACCGTTGAAGAAGGCTCGGGCCTGGAAAACGAACTGTCCGTCGTAGAAGGCATGCAGTTCGACCGCGGCTACCTGTCGCCGTACTTCGTc                                                                                                          <  1:4924865/150‑1 (MQ=255)

                                               cTCCATCGGTGAAATCATCGCCGAAGCCATGGAAAAAGTCGGTAAAGAAGGCGTGATCACCGTTGAAGAAGGCTCGGGCCTGGAAAACGAACTGTCCGTCGTAGAAGGCATGCAGTTCGACCGCGGCTACCTGTCGCCGTACTTCGTcaa                                                                                                        <  2:747816/150‑1 (MQ=255)

                                                 ccATCGGTGAAATCATCGCCGAAGCCATGGAAAAAGTCGGTAAAGAAGGCGTGATCACCGTTGAAGAAGGCTCGGGCCTGGAAAACGAACTGTCCGTCGTAGAAGGCATGCAGTTCGACCGCGGCTACCTGTCGCCGTACTTCGTcaaca                                                                                                      >  2:586285/1‑150 (MQ=255)

                                                   aTCGGTGAAATCATCGCCGAAGCCATGGAAAAAGTCGGTAAAGAAGGCGTGATCACCGTTGAAGAAGGCTCGGGCCTGGAAAACGAACTGTCCGTCGTAGAAGGCATGCAGTTCGACCGCGGCTACCTGTCGCCGTACTTCGTCAACAAg                                                                                                    >  1:2662799/1‑150 (MQ=255)

                                                       gTGAAATCATCGCCGAAGCCATGGAAAAAGTCGGTAAAGAAGGCGTGATCACCGTTGAAGAAGGCTCGGGCCTGGAAAACGAACTGTCCGTCGTAGAAGGCATGCAGTTCGACCGCGGCTACCTGTCGCCGTACTTCGTCAACAAGCCgg                                                                                                >  1:463132/1‑150 (MQ=255)

                                                        tGAAATCATCGCCGAAGCCATGGAAAAAGTCGGTAAAGAAGGCGTGATCACCGTTGAAGAAGGCTCGGGCCTGGAAAACGAACTGTCCGTCGTAGAAGGCATGCAGTTCGACCGCGGCTACCTGTCGCCGTACTTCGTCAACAAGCCGGa                                                                                               <  1:2649368/150‑1 (MQ=255)

                                                            atcatcGCCGAAGCCATGGAAAAAGTCGGTAAAGAAGGCGTGATCACCGTTGAAGAAGGCTCGGGCCTGGAAAACGAACTGTCCGTCGTAGAAGGCATGCAGTTCGACCGCGGCTACCTGTCGCCGTACTTCGTCAACAAGCCGGACAcc                                                                                           <  1:4638977/150‑1 (MQ=255)

                                                            atcatcGCCGAAGCCATGGAAAAAGTCGGTAAAGAAGGCGTGATCACCGTTGAAGAAGGCTCGGGCCTGGAAAACGAACTGTCCGTCGTAGAAGGCATGCAGTTCGACCGCGGCTACCTGTCGCCGTACTTCGTCAACAAGCCGGACAcc                                                                                           <  1:4638923/150‑1 (MQ=255)

                                                            atcatcGCCGAAGCCATGGAAAAAGTCGGTAAAGAAGGCGTGATCACCGTTGAAGAAGGCTCGGGCCTGGAAAACGAACTGTCCGTCGTAGAAGGCATGCAGTTCGACCGCGGCTACCTGTCGCCGTACTTCGTCAACAAGCCGGACAcc                                                                                           >  1:4257527/1‑150 (MQ=255)

                                                               atcGCCGAAGCCATGGAAAAAGTCGGTAAAGAAGGCGTGATCACCGTTGAAGAAGGCTCGGGCCTGGAAAACGAACTGTCCGTCGTAGAAGGCATGCAGTTCGACCGCGGCTACCTGTCGCCGTACTTCGTCAACAAGCCGGACACCATg                                                                                        >  2:3248376/1‑150 (MQ=255)

                                                                tcGCCGAAGCCATGGAAAAAGTCGGTAAAGAAGGCGTGATCACCGTTGAAGAAGGCTCGGGCCTGGAAAACGAACTGTCCGTCGTAGAAGGCATGCAGTTCGACCGCGGCTACCTGTCGCCGTACTTCGTCAACAAGCCGGACACCATgg                                                                                       >  2:1581516/1‑150 (MQ=255)

                                                                tcGCCGAAGCCATGGAAAAAGTCGGTAAAGAAGGCGTGATCACCGTTGAAGAAGGCTCGGGCCTGGAAAACGAACTGTCCGTCGTAGAAGGCATGCAGTTCGACCGCGGCTACCTGTCGCCGTACTTCGTCAACAAGCCGGACACCATgg                                                                                       >  2:4373908/1‑150 (MQ=255)

                                                                tcGCCGAAGCCATGGAAAAAGTCGGTAAAGAAGGCGTGATCACCGTTGAAGAAGGCTCGGGCCTGGAAAACGAACTGTCCGTCGTAGAAGGCATGCAGTTCGACCGCGGCTACCTGTCGCCGTACTTCGTCAACAAGCCGGACACCATgg                                                                                       >  1:4838727/1‑150 (MQ=255)

                                                                  gctgAAGCGATGCAGAAGGTTGGCAACGAAGGCGTCATCACGGTTGAAGAAGCCAAGACCGCTGAAACCGAACTCGAAGTCGTTGAAGGCATGCAGTTCGACCGCGGCTACCTGTCGCCATACTTCGTCACCAACCCAGACAAGATGGtt                                                                                     >  1:375046/4‑150 (MQ=255)

                                                                  gctgAAGCGATGCAGAAGGTTGGCAACGAAGGCGTCATCACGGTTGAAGAAGCCAAGACCGCTGAAACCGAACTCGAAGTCGTTGAAGGCATGCAGTTCGACCGCGGCTACCTGTCGCCATACTTCGTCACCAACCCAGACAAGATGGtt                                                                                     <  1:708264/147‑1 (MQ=255)

                                                                  gctgAAGCGATGCAGAAGGTTGGCAACGAAGGCGTCATCACGGTTGAAGAAGCCAAGACCGCTGAAACCGAACTCGAAGTCGTTGAAGGCATGCAGTTCGACCGCGGCTACCTGTCGCCATACTTCGTCACCAACCCAGACAAGATGGtt                                                                                     >  2:1640083/4‑150 (MQ=255)

                                                                     gAAGCCATGGAAAAAGTCGGTAAAGAAGGCGTGATCACCGTTGAAGAAGGCTCGGGCCTGGAAAACGAACTGTCCGTCGTAGAAGGCATGCAGTTCGACCGCGGCTACCTGTCGCCGTACTTCGTCAACAAGCCGGACACCATGGTTGcc                                                                                  <  2:357938/150‑1 (MQ=255)

                                                                     gAAGCCATGGAAAAAGTCGGTAAAGAAGGCGTGATCACCGTTGAAGAAGGCTCGGGCCTGGAAAACGAACTGTCCGTCGTAGAAGGCATGCAGTTCGACCGCGGCTACCTGTCGCCGTACTTCGTCAACAAGCCGGACACCATGGTTGcc                                                                                  >  1:2190167/1‑150 (MQ=255)

                                                                      aaGCCATGGAAAAAGTCGGTAAAGAAGGCGTGATCACCGTTGAAGAAGGCTCGGGCCTGGAAAACGAACTGTCCGTCGTAGAAGGCATGCAGTTCGACCGCGGCTACCTGTCGCCGTACTTCGTCAACAAGCCGGACACCATGGTTGCCg                                                                                 >  1:1799413/1‑150 (MQ=255)

                                                                         ccATGGAAAAAGTCGGTAAAGAAGGCGTGATCACCGTTGAAGAAGGCTCGGGCCTGGAAAACGAACTGTCCGTCGTAGAAGGCATGCAGTTCGACCGCGGCTACCTGTCGCCGTACTTCGTCAACAAGCCGGACACCATGGTTGCCGAGc                                                                              >  1:4543610/1‑150 (MQ=255)

                                                                         ccATGGAAAAAGTCGGTAAAGAAGGCGTGATCACCGTTGAAGAAGGCTCGGGCCTGGAAAACGAACTGTCCGTCGTAGAAGGCATGCAGTTCGACCGCGGCTACCTGTCGCCGTACTTCGTCAACAAGCCGGACACCATGGTTGCCGAGc                                                                              <  2:2207475/150‑1 (MQ=255)

                                                                          cATGGAAAAAGTCGGTAAAGAAGGCGTGATCACCGTTGAAGAAGGCTCGGGCCTGGAAAACGAACTGTCCGTCGTAGAAGGCATGCAGTTCGACCGCGGCTACCTGTCGCCGTACTTCGTCAACAAGCCGGACACCATGGTTGCCGAGCt                                                                             >  1:2072622/1‑150 (MQ=255)

                                                                              gAAAAAGTCGGTAAAGAAGGCGTGATCACCGTTGAAGAAGGCTCGGGCCTGGAAAACGAACTGTCCGTCGTAGAAGGCATGCAGTTCGACCGCGGCTACCTGTCGCCGTACTTCGTCAACAAGCCGGACACCATGGTTGCCGAGCTGGaa                                                                         >  2:372604/1‑150 (MQ=255)

                                                                              gAAAAAGTCGGTAAAGAAGGCGTGATCACCGTTGAAGAAGGCTCGGGCCTGGAAAACGAACTGTCCGTCGTAGAAGGCATGCAGTTCGACCGCGGCTACCTGTCGCCGTACTTCGTCAACAAGCCGGACACCATGGTTGCCGAGCTGGaa                                                                         <  2:3365230/150‑1 (MQ=255)

                                                                              gAAAAAGTCGGTAAAGAAGGCGTGATCACCGTTGAAGAAGGCTCGGGCCTGGAAAACGAACTGTCCGTCGTAGAAGGCATGCAGTTCGACCGCGGCTACCTGTCGCCGTACTTCGTCAACAAGCCGGACACCATGGTTGCCGAGCTGGaa                                                                         <  2:5061170/150‑1 (MQ=255)

                                                                              gAAAAAGTCGGTAAAGAAGGCGTGATCACCGTTGAAGAAGGCTCGGGCCTGGAAAACGAACTGTCCGTCGTAGAAGGCATGCAGTTCGACCGCGGCTACCTGTCGCCGTACTTCGTCAACAAGCCGGACACCATGGTTGCCGAGCTGGaa                                                                         <  1:1549250/150‑1 (MQ=255)

                                                                               aaaaaGTCGGTAAAGAAGGCGTGATCACCGTTGAAGAAGGCTCGGGCCTGGAAAACGAACTCTCCGTCGTAGAAGGCATGCAGTTCGACCGCGGCTACCTGTCGCCGTACTTCGTCAACAAGCCGGACACCATGGCTGCCGAGCTGGAAg                                                                        <  1:198000/150‑1 (MQ=255)

                                                                                   aGTCGGTAAAGAAGGCGTGATCACCGTTGAAGAAGGCTCGGGCCTGGAAAACGAACTGTCCGTCGTAGAAGGCATGCAGTTCGACCGCGGCTACCTGTCGCCGTACTTCGTCAACAAGCCGGACACCATGGTTGCCGAGCTGGAAGGccc                                                                    <  2:2434435/150‑1 (MQ=255)

                                                                                     tCGGTAAAGAAGGCGTGATTACCGTTGAAGAAGGCTCGGGCCTGGAAAACGAACTGTCCGTCGTAGAAGGCATGCAGTTCGACCGCGGCTACCTGTCGCCGTACTTCGTCAACAAGCCGGACACCATGGTTGCCGAGCTGGAAGGCCCgc                                                                  >  1:5029348/1‑150 (MQ=255)

                                                                                     tCGGTAAAGAAGGCGTGATCACCGTTGAAGAAGGCTCGGGCCTGGAAAACGAACTGTCCGTCGTAGAAGGCATGCAGTTCGACCGCGGCTACCTGTCGCCGTACTTCGTCAACAAGCCGGACACCATGGTTGCCGAGCTGGAAGGCCCgc                                                                  <  2:2719932/150‑1 (MQ=255)

                                                                                     tCGGTAAAGAAGGCGTGATCACCGTTGAAGAAGGCTCGGGCCTGGAAAACGAACTGTCCGTCGTAGAAGGCATGCAGTTCGACCGCGGCTACCTGTCGCCGTACTTCGTCAACAAGCCGGACACCATGGTTGCCGAGCTGGAAGGCCCgc                                                                  <  2:1413316/150‑1 (MQ=255)

                                                                                      cGGTTAAGAAGGCGTGATCACCGTTGAAGAAGGCTCGGGCCTGGAAAACGAACTGTCCGTCGTAGAAGGCATGCAGTTCGACCGCGGCTACCTGTCGCCGTACTTCGTCAACAAGCCGGACACCATGGTTGCCGAGCTGGAAGGCCCgct                                                                 <  1:4461255/150‑1 (MQ=255)

                                                                                           aagaagGCGTGATCACCGTTGAAGAAGGCTCGGGCCTGGAAAACGAACTGTCCGTCGTAGAAGGCATGCAGTTCGACCGCGGCTACCTGTCGCCGTACTTCGTCAACAAGCCGGACACCATGGTTGCCGAGCTGGAAGGCCCgctgctgc                                                            <  1:1042920/150‑1 (MQ=255)

                                                                                                      aTCACCGTTGAAGAAGGCTCGGGCCTGGAAAACGAACTGTCCGTCGTAGAAGGCATGCAGTTCGACCGCGGCTACCTGTCGCCGGACTTCGTCAACAAGCCGGACACCATGGTTGCCGAGCTGGAAGGCCCGCTGCTGCTGCTGGTCGAc                                                 <  2:411710/150‑1 (MQ=255)

                                                                                                        cACCGTTGAAGAAGGCTCGGGCCTGGAAAACGAACTGTCCGTCGTAGAAGGCATGCAGTTCGACCGCGGCTACCTGTCGCCGTACTTCGTCAACAAGCCGGACACCATGGTTGCCGAGCTGGAAGGCCCGCTGCTGCTGCTGGTCGACaa                                               >  1:4585825/1‑150 (MQ=255)

                                                                                                         aCCGTTGAAGAAGGCTCGGGCCTGGAAAACGAACTGTCCGTCGTAGAAGGCATGCAGTTCGACCGCGGCTACCTGTCGCCGTACTTCGTCAACAAGCCGGACACCATGGTTGCCGAGCTGGAAGGCCCGCTGCTGCTGCTGGTCGACAAg                                              >  1:2734522/1‑150 (MQ=255)

                                                                                                          ccGTTGAAGAAGGCTCGGGCCTGGAAAACGAACTGTCCGTCGTAGAAGGCATGCAGTTCGACCGCGGCTACCTGTCGCCGTACTTCGTCAACAAGCCGGACACCATGGTTGCCGAGCTGGAAGGCCCGCTGCTGCTGCTGGTCGACAAGa                                             <  2:2061347/150‑1 (MQ=255)

                                                                                                          ccGTTGAAGAAGGCTCGGGCCTGGAAAACGAACTGTCCGTCGTAGAAGGCATGCAGTTCGACCGCGGCTACCTGTCGCCGTACTTCGTCAACAAGCCGGACACCATGGTTGCCGAGCTGGAAGGCCCGCTGCTGCTGCTGGTCGACAAGa                                             >  1:1020613/1‑150 (MQ=255)

                                                                                                            gTTGAAGAAGGCTCGGGCCTGGAAAACGAACTGTCCGTCGTAGAAGGCATGCAGTTCGACCGCGGCTACCTGTCGCCGTACTTCGTCAACAAGCCGGACACCATGGTTGCCGAGCTGGAAGGCCCGCTGCTGCTGCTGGTCGACAAGaaa                                           >  1:509875/1‑150 (MQ=255)

                                                                                                               gaagaaGGCTCGGGCCTGGAAAACGAACTGTCCGTCGTAGAAGGCATGCAGTTCGACCGCGGCTACCTGTCGCCGTACTTCGTCAACAAGCCGGACACCATGGTTGCCGAGCTGGAAGGCCCGCTGCTGCTGCTGGTCGACAAGAAAAtc                                        <  1:877663/150‑1 (MQ=255)

                                                                                                                aagaagGCTCGGGCCTGGAAAACGAACTGTCCGTCGTAGAAGGCATGCAGTTCGACCGCGGCTACCTGTCGCCGTACTTCGTCAACAAGCCGGACACCATGGTTGCCGAGCTGGAAGGCCCGCTGCTGCTGCTGGTCGACACGAAAAtct                                       >  2:1212196/1‑150 (MQ=255)

                                                                                                                      gCTCGGGCCTGGAAAACGAACTGTCCGTCGTAGAAGGCATGCAGTTCGACCGCGGCTACCTGTCGCCGTACTTCGTCAACAAGCCGGACACCATGGTTGCCGAGCTGGAAGGCCCGCTGCTGCTGCTGGTCGACAAGAAAATCTCCAACa                                 >  2:1517862/1‑150 (MQ=255)

                                                                                                                        tCGGGCCTGGAAAACGAACTGTCCGTCGTAGAAGGCATGCAGTTCGACCGCGGCTACCTATCGCCGTACTTCGTCAACAAGCCGGACACCATGGTTGCCGAGCTGGAAGGCCCGCTGCTGCTGCTGGTCGACAAGAAAATCTCCAACATc                               >  1:2278669/1‑150 (MQ=255)

                                                                                                                         cGGGCCTGGAAAACGAACTGTCCGTCGTAGAAGGCATGCAGTTCGACCGCGGCTACCTGTCGCCGTACTTCGTCAACAAGCCGGACACCATGGTTGCCGAGCTGGAAGGCCCGCTGCTGCTGCTGGTCGACAAGAAAATCTCCAACATcc                              <  2:2244103/150‑1 (MQ=255)

                                                                                                                         cGGGCCTGGAAAACGAACTGTCCGTCGTAGAAGGCATGCAGTTCGACCGCGGCTACCTGTCGCCGTACTTCGTCAACAAGCCGGACACCATGGTTGCCGAGCTGGAAGGCCCGCTGCTGCTGCTGGTCGACAAGAAAATCTCCAACATcc                              <  2:1514774/150‑1 (MQ=255)

                                                                                                                           ggCCTGGAAAACGAACTGTCCGTCGTAGAAGGCATGCAGTTCGACCGCGGCTACCTGTCGCCGTACTTCGTCAACAAGCCGGACACCATGGTTGCCGAGCTGGAAGGCCCGCTGCTGCTGCTGGTCGACAAGAAAATCTCCAACATCCGt                            <  1:2593070/150‑1 (MQ=255)

                                                                                                                               tGGAAAACGAACTGTCCGTCGTAGAAGGCATGCAGTTCGACCGCGGCTACCTGTCGCCGTACTTCGTCAACAAGCCGGACACCATGGTTGCCGAGCTGGAAGGCCCGCTGCTGCTGCTGGTCGACAAGAAAATCTCCAACATCCGTGAgc                        <  2:3025222/150‑1 (MQ=255)

                                                                                                                                   aaaCGAACTGTCCGTCGTAGAAGGCATGCAGTTCGACCGCGGCTACCTGTCGCCGTACTTCGTCAACAAGCCGGACACCATGGTTGCCGAGCTGGAAGGCCCGCTGCTGCTGCTGGTCGACAAGAAAATCTCCAACATCCGTGAgctgct                    >  2:346314/1‑150 (MQ=255)

                                                                                                                                    aaCGAACTGTCCGTCGTAGAAGGCATGCAGTTCGACCGCGGCTACCTGTCGCCGTACTTCGTCAACAAGCCGGACACCATGGTTGCCGAGCTGGAAGGCCCGCTGCTGCTGCTGGTCGACAAGAAAATCTCCAACATCCGTGAGctgctg                   >  1:5181232/1‑150 (MQ=255)

                                                                                                                                     aCGAACTGTCCGTCGTAGAAGGCATGCAGTTCGACCGCGGCTACCTGTCGCCGTACTTCGTCAACAAGCCGGACACCATGGTTGCCGAg                                                                               <  2:1376258/89‑1 (MQ=255)

                                                                                                                                     aCGAACTGTCCGTCGTAGAAGGCATGCAGTTCGACCGCGGCTACCTGTCGCCGTACTTCGTCAACAAGCCGGACACCATGGTTGCCGAg                                                                               >  1:1376258/1‑89 (MQ=255)

                                                                                                                                     aCGAACTGTCCGTCGTAGAAGGCATGCAGTTCGACCGCGGCTACCTGTCGCCGTACTTCGTCAACAAGCCGGACACCATGGTTGCCGAGCTGGAAGGCCCGCTGCTGCTGCTGGTCGACAAGAAAATCTCCAACATCCGTGAGCtgctgc                  <  2:1700604/150‑1 (MQ=255)

                                                                                                                                     aCGAACTGTCCGTCGTAGAAGGCATGCAGTTCGACCGCGGCTACCTGTCGCCGTACTTCGTCAACAAGCCGGACACCATGGTTGCCGAGCTGGAAGGCCCGCTGCTGCTGCTGGTCGACAAGAAAATCTCCAACATCCGTGAGCtgctgc                  <  1:3730789/150‑1 (MQ=255)

                                                                                                                                         aCTGTCCGTCGTAGAAGGCATGCAGTTCGACCGCGGCTACCTGTCGCCGTACTTCGTCAACAAGCCGGACACCATGGTTGCCGAGCTGGAAGGCCCGctgctgctgctg                                                       >  2:4974126/1‑109 (MQ=255)

                                                                                                                                         aCTGTCCGTCGTAGAAGGCATGCAGTTCGACCGCGGCTACCTGTCGCCGTACTTCGTCAACAAGCCGGACACCATGGTTGCCGAGCTGGAAGGCCCGctgctgctgctg                                                       <  1:4974126/109‑1 (MQ=255)

                                                                                                                                           tGTCCGTCGTAGAAGGCATGCAGTTCGACCGCGGCTACCTGTCGCCGTACTTCGTCAACAAGCCGGACACCATGGTTGCCGAGCTGGAAGGCCCGCTGCTGCTGCTGGTCGACAAGAAAATCTCCAACATCCGTGAGCTGCTGCCAGTTc            >  1:817423/1‑150 (MQ=255)

                                                                                                                                           tGTCCGTCGTAGAAGGCATGCAGTTCGACCGCGGCTACCTGTCGCCGTACTTCGTCAACAAGCCGGACACCATGGTTGCCGAGCTGGAAGGCCCGCTGCTGCTGCTGGTCGACAAGAAAATCTCCAACATCCGTGAGCTGCTGCCAGTTc            <  1:4569508/150‑1 (MQ=255)

                                                                                                                                                gtcgtAGAAGGCATGCAGTTCGACCGCGGCTACCTGTCGCCGTACTTCGTCAACAAGCCGGACACCATGGTTGCCGAGCTGGAAGGCCCGCTGCTGCTGCTGGTCGACAAGAAAATCTCCAACATCCGTGAGCTGCTGCCAGTTCTGGaa       <  1:472586/150‑1 (MQ=255)

                                                                                                                                                 tcgtAGAAGGCATGCAGTTCGACCGCGGCTACCTGTCGCCGTACTTCGTCAACAAGCCGGACACCATGGTTGCCGAGCTGGAAGGCCCGCTGCTGCTGCTGGTCGACAAGAAAATCTCCAACATCCGTGAGCTGCTGCCAGTTCTGGAAg      >  2:884232/1‑150 (MQ=255)

                                                                                                                                                  cgtAGAAGGCATGCAGTTCGACCGCGGCTACCTGTCGCCGTACTTCGTCAACAAGCCGGACACCATGGTTGCCGAGCTGGAAGGCCCGCTGCTGCTGCTGGTCGACAAGAAAATCTCCAACATCCGTGAGCTGCTGCCAGTTCTGGAAGc     <  2:3591730/150‑1 (MQ=255)

                                                                                                                                                   gtAGAAGGCATGCAGTTCGACCGCGGCTACCTGTCGCCGTACTTCGTCAACAAGCCGGACACCATGGTTGCCGAGCTGGAAGGCCCGCTGCTGCTGCTGGTCGACAAGAAAATCTCCAACATCCGTGAGCTGCTGCCAGTTCTGGAAGcc    <  1:3525968/150‑1 (MQ=255)

                                                                                                                                                    tAGAAGGCATGCAGTTCGACCGCGGCTACCTGTCGCCGTACTTCGTCAACAAGCCGGACACCATGGTTGCCGAGCTGGAAGGCCCGCTGCTGCTGCTGGTCGACAAGAAAATCTCCAACATCCGTGAGCTGCTGCCAGTTCTGGAAGCCg   >  2:1991931/1‑150 (MQ=255)

                                                                                                                                                    tAGAAGGCATGCAGTTCGACCGCGGCTACCTGTCGCCGTACTTCGTCAACAAGCCGGACACCATGGTTGCCGAGCTGGAAGGCCCGCTGCTGCTGCTGGTCGACAAGAAAATCTCCAACATCCGTGAGCTGCTGCCAGTTCTGGAAGCCg   >  2:5172331/1‑150 (MQ=255)

                                                                                                                                                     aGAAGGCATGCAGTTCGACCGCGGCTACCTGTCGCCGTACTTCGTCAACAAGCCGGACACCATGGTTGCCGAGCTGGAAGGCCCGCTGCTGCTGCTGGTCGACAAGAAAATCTCCAACATCCGTGAGCTGCTGCCAGTTCTGGAAGCCGt  >  2:4628122/1‑150 (MQ=255)

TCCAAGGCCATCGCCCAGGTAGGTACCATCTCTGCCAACTCCGACAACTCCATCGGTGAAATCATCGCCGAAGCCATGGAAAAAGTCGGTAAAGAAGGCGTGATCACCGTTGAAGAAGGCTCGGGCCTGGAAAACGAACTGTCCGTCGTAGAAGGCATGCAGTTCGACCGCGGCTACCTGTCGCCGTACTTCGTCAACAAGCCGGACACCATGGTTGCCGAGCTGGAAGGCCCGCTGCTGCTGCTGGTCGACAAGAAAATCTCCAACATCCGTGAGCTGCTGCCAGTTCTGGAAGCCGT  >  NC_002947/1550063‑1550361

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`