BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	1,550,266	G→A	6.2%	P208P (CCG→CCA)	groL →	chaperonin GroEL

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	1,550,266	0	G	A	6.2%	401.0 / 11.0	132	P208P (CCG→CCA)	groL	chaperonin GroEL
Reads supporting (aligned to +/- strand): ref base G (59/64); new base A (5/3); total (64/68)
Fisher's exact test for biased strand distribution p-value = 4.86e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.38e-01

Only 100 of 134 total aligned reads displayed.

GTGAAATCATCGCCGAAGCCATGGAAAAAGTCGGTAAAGAAGGCGTGATCACCGTTGAAGAAGGCTCGGGCCTGGAAAACGAACTGTCCGTCGTAGAAGGCATGCAGTTCGACCGCGGCTACCTGTCGCCGTACTTCGTCAACAAGCCGGACACCATGGTTGCCGAGCTGGAAGGCCCGCTGCTGCTGCTGGTCGACAAGAAAATCTCCAACATCCGTGAGCTGCTGCCAGTTCTGGAAGCCGTTGCCAAGGCCGGCCGCCCACTGCTGATCGTTGCCGAAGACGTTGAAGGTGAAGC  >  NC_002947/1550118‑1550415

 tGAAATCATCGCCGAAGCCATGGAAAAAGTCGGTAAAGAAGGCGTGATCACCGTTGAAGAAGGCTCGGGCCTGGAAAACGAACTGTCCGTCGTAGAAGGCATGCAGTTCGACCGCGGCTACCTGTCGCCGTACTTCGTCAACAAGCCGGa                                                                                                                                                     <  1:2649368/150‑1 (MQ=255)

 tGAAATCATCGCCGAAGCCATGGAAAAAGTCGGTAAAGAAGGCGTGATCACCGTTGAAGAAGGCTCGGGCCTGGAAAACGAACTGTCCGTCGTAGAAGGCATGCAGTTCGACCGCGGCTACCTGTCGCCGTACTTCGTCAACAAGCCGGa                                                                                                                                                     <  2:1483914/150‑1 (MQ=255)

     atcatcGCCGAAGCCATGGAAAAAGTCGGTAAAGAAGGCGTGATCACCGTTGAAGAAGGCTCGGGCCTGGAAAACGAACTGTCCGTCGTAGAAGGCATGCAGTTCGACCGCGGCTACCTGTCGCCGTACTTCGTCAACAAGCCGGACAcc                                                                                                                                                 <  1:4638977/150‑1 (MQ=255)

     atcatcGCCGAAGCCATGGAAAAAGTCGGTAAAGAAGGCGTGATCACCGTTGAAGAAGGCTCGGGCCTGGAAAACGAACTGTCCGTCGTAGAAGGCATGCAGTTCGACCGCGGCTACCTGTCGCCGTACTTCGTCAACAAGCCGGACAcc                                                                                                                                                 <  1:4638923/150‑1 (MQ=255)

     atcatcGCCGAAGCCATGGAAAAAGTCGGTAAAGAAGGCGTGATCACCGTTGAAGAAGGCTCGGGCCTGGAAAACGAACTGTCCGTCGTAGAAGGCATGCAGTTCGACCGCGGCTACCTGTCGCCGTACTTCGTCAACAAGCCGGACAcc                                                                                                                                                 <  1:4638657/150‑1 (MQ=255)

     atcatcGCCGAAGCCATGGAAAAAGTCGGTAAAGAAGGCGTGATCACCGTTGAAGAAGGCTCGGGCCTGGAAAACGAACTGTCCGTCGTAGAAGGCATGCAGTTCGACCGCGGCTACCTGTCGCCGTACTTCGTCAACAAGCCGGACAcc                                                                                                                                                 >  1:4257527/1‑150 (MQ=255)

        atcGCTGAAGCGATGCAGAAGGTTGGCAACGAAGGCGTCATCACGGTTGAAGAAGCCAAGACCGCTGAAACCGAACTCGAAGTCGTTGAAGGCATGCAGTTCGACCGCGGCTACCTGTCGCCATACTTCGTCACCAACCCAGacaagatg                                                                                                                                              >  2:3704644/1‑145 (MQ=255)

        atcGCCGAAGCCATGGAAAAAGTCGGTAAAGAAGGCGTGATCACCGTTGAAGAAGGCTCGGGCCTGGAAAACGAACTGTCCGTCGTAGAAGGCATGCAGTTCGACCGCGGCTACCTGTCGCCGTACTTCGTCAACAAGCCGGACACCATg                                                                                                                                              <  1:4957426/150‑1 (MQ=255)

        atcGCCGAAGCCATGGAAAAAGTCGGTAAAGAAGGCGTGATCACCGTTGAAGAAGGCTCGGGCCTGGAAAACGAACTGTCCGTCGTAGAAGGCATGCAGTTCGACCGCGGCTACCTGTCGCCGTACTTCGTCAACAAGCCGGACACCATg                                                                                                                                              >  2:3248376/1‑150 (MQ=255)

         tcGCCGAAGCCATGGAAAAAGTCGGTAAAGAAGGCGTGATCACCGTTGAAGAAGGCTCGGGCCTGGAAAACGAACTGTCCGTCGTAGAAGGCATGCAGTTCGACCGCGGCTACCTGTCGCCGTACTTCGTCAACAAGCCGGACACCATgg                                                                                                                                             >  1:2598555/1‑150 (MQ=255)

         tcGCCGAAGCCATGGAAAAAGTCGGTAAAGAAGGCGTGATCACCGTTGAAGAAGGCTCGGGCCTGGAAAACGAACTGTCCGTCGTAGAAGGCATGCAGTTCGACCGCGGCTACCTGTCGCCGTACTTCGTCAACAAGCCGGACACCATgg                                                                                                                                             >  2:4373908/1‑150 (MQ=255)

         tcGCCGAAGCCATGGAAAAAGTCGGTAAAGAAGGCGTGATCACCGTTGAAGAAGGCTCGGGCCTGGAAAACGAACTGTCCGTCGTAGAAGGCATGCAGTTCGACCGCGGCTACCTGTCGCCGTACTTCGTCAACAAGCCGGACACCATgg                                                                                                                                             >  2:1581516/1‑150 (MQ=255)

         tcGCCGAAGCCATGGAAAAAGTCGGTAAAGAAGGCGTGATCACCGTTGAAGAAGGCTCGGGCCTGGAAAACGAACTGTCCGTCGTAGAAGGCATGCAGTTCGACCGCGGCTACCTGTCGCCGTACTTCGTCAACAAGCCGGACACCATgg                                                                                                                                             >  1:1405263/1‑150 (MQ=255)

         tcGCCGAAGCCATGGAAAAAGTCGGTAAAGAAGGCGTGATCACCGTTGAAGAAGGCTCGGGCCTGGAAAACGAACTGTCCGTCGTAGAAGGCATGCAGTTCGACCGCGGCTACCTGTCGCCGTACTTCGTCAACAAGCCGGACACCATgg                                                                                                                                             >  1:4838727/1‑150 (MQ=255)

           gctgAAGCGATGCAGAAGGTTGGCAACGAAGGCGTCATCACGGTTGAAGAAGCCAAGACCGCTGAAACCGAACTCGAAGTCGTTGAAGGCATGCAGTTCGACCGCGGCTACCTGTCGCCATACTTCGTCACCAACCCAGACAAGATGGtt                                                                                                                                           >  2:1640083/4‑150 (MQ=255)

           gctgAAGCGATGCAGAAGGTTGGCAACGAAGGCGTCATCACGGTTGAAGAAGCCAAGACCGCTGAAACCGAACTCGAAGTCGTTGAAGGCATGCAGTTCGACCGCGGCTACCTGTCGCCATACTTCGTCACCAACCCAGACAAGATGGtt                                                                                                                                           >  2:1640099/4‑150 (MQ=255)

           gctgAAGCGATGCAGAAGGTTGGCAACGAAGGCGTCATCACGGTTGAAGAAGCCAAGACCGCTGAAACCGAACTCGAAGTCGTTGAAGGCATGCAGTTCGACCGCGGCTACCTGTCGCCATACTTCGTCACCAACCCAGACAAGATGGtt                                                                                                                                           <  1:708264/147‑1 (MQ=255)

             tgAAGCGATGCAGAAGGTTGGCAACGAAGGCGTCATCACGGTTGAAGAAGCCAAGACCGCTGAAACCGAACTCGAAGTCGTTGAAGGCATGCAGTTCGACCGCGGCTACCTGTCGCCATACTTCGTCACCAACCCAGACAAGATGGTTGc                                                                                                                                         <  2:1206031/149‑1 (MQ=255)

             cGAAGCCATGGAAAAAGTCGGTAAAGAAGGCGTGATCACCGTTGAAGAAGGCTCGGGCCTGGAAAACGAACTGTCCGTCGTAGAAGGCATGCAGTTCGACCGCGGCTACCTGTCGCCGTACTTCGTCAACAAGCCGGACACCATGGTTGc                                                                                                                                         >  1:754393/1‑150 (MQ=255)

              gAAGCCATGGAAAAAGTCGGTAAAGAAGGCGTGATCACCGTTGAAGAAGGCTCGGGCCTGGAAAACGAACTGTCCGTCGTAGAAGGCATGCAGTTCGACCGCGGCTACCTGTCGCCGTACTTCGTCAACAAGCCGGACACCATGGTTGcc                                                                                                                                        <  2:357938/150‑1 (MQ=255)

               aaGCCATGGAAAAAGTCGGTAAAGAAGGCGTGATCACCGTTGAAGAAGGCTCGGGCCTGGAAAACGAACTGTCCGTCGTAGAAGGCATGCAGTTCGACCGCGGCTACCTGTCGCCGTACTTCGTCAACAAGCCGGACACCATGGTTGCCg                                                                                                                                       >  1:1799413/1‑150 (MQ=255)

                  ccATGGAAAAAGTCGGTAAAGAAGGCGTGATCACCGTTGAAGAAGGCTCGGGCCTGGAAAACGAACTGTCCGTCGTAGAAGGCATGCAGTTCGACCGCGGCTACCTGTCGCCGTACTTCGTCAACAAGCCGGACACCATGGTTGCCGAGc                                                                                                                                    <  2:2207475/150‑1 (MQ=255)

                  ccATGGAAAAAGTCGGTAAAGAAGGCGTGATCACCGTTGAAGAAGGCTCGGGCCTGGAAAACGAACTGTCCGTCGTAGAAGGCATGCAGTTCGACCGCGGCTACCTGTCGCCGTACTTCGTCAACAAGCCGGACACCATGGTTGCCGAGc                                                                                                                                    >  1:4543610/1‑150 (MQ=255)

                   cATGGAAAAAGTCGGTAAAGAAGGCGTGATCACCGTTGAAGAAGGCTCGGGCCTGGAAAACGAACTGTCCGTCGTAGAAGGCATGCAGTTCGACCGCGGCTACCTGTCGCCGTACTTCGTCAACAAGCCGGACACCATGGTTGCCGAGCt                                                                                                                                   >  1:2072622/1‑150 (MQ=255)

                       gAAAAAGTCGGTAAAGAAGGCGTGATCACCGTTGAAGAAGGCTCGGGCCTGGAAAACGAACTGTCCGTCGTAGAAGGCATGCAGTTCGACCGCGGCTACCTGTCGCCGTACTTCGTCAACAAGCCGGACACCATGGTTGCCGAGCTGGaa                                                                                                                               <  2:3365230/150‑1 (MQ=255)

                       gAAAAAGTCGGTAAAGAAGGCGTGATCACCGTTGAAGAAGGCTCGGGCCTGGAAAACGAACTGTCCGTCGTAGAAGGCATGCAGTTCGACCGCGGCTACCTGTCGCCGTACTTCGTCAACAAGCCGGACACCATGGTTGCCGAGCTGGaa                                                                                                                               >  2:372604/1‑150 (MQ=255)

                       gAAAAAGTCGGTAAAGAAGGCGTGATCACCGTTGAAGAAGGCTCGGGCCTGGAAAACGAACTGTCCGTCGTAGAAGGCATGCAGTTCGACCGCGGCTACCTGTCGCCGTACTTCGTCAACAAGCCGGACACCATGGTTGCCGAGCTGGaa                                                                                                                               <  1:1549250/150‑1 (MQ=255)

                       gAAAAAGTCGGTAAAGAAGGCGTGATCACCGTTGAAGAAGGCTCGGGCCTGGAAAACGAACTGTCCGTCGTAGAAGGCATGCAGTTCGACCGCGGCTACCTGTCGCCGTACTTCGTCAACAAGCCGGACACCATGGTTGCCGAGCTGGaa                                                                                                                               <  2:5061170/150‑1 (MQ=255)

                          aaaGTCGGTAAAGAAGGCGTGATCACCGTTGAAGAAGGCTCGGGCCTGGAAAACGAACTGTCCGTCGTAGAAGGCATGCAGTTCGACCGCGGCTGCCTGTCGCCGTACTTCGTCAACAAGCCGGACACCATGGTTGCCGAGCTGGAAGGc                                                                                                                            >  1:3266636/1‑150 (MQ=255)

                            aGTCGGTAAAGAAGGCGTGATCACCGTTGAAGAAGGCTCGGGCCTGGAAAACGAACTGTCCGTCGTAGAAGGCATGCAGTTCGACCGCGGCTACCTGTCGCCGTACTTCGTCAACAAGCCGGACACCATGGTTGCCGAGCTGGAAGGccc                                                                                                                          <  2:2357134/150‑1 (MQ=255)

                              tCGGTAAAGAAGGCGTGATTACCGTTGAAGAAGGCTCGGGCCTGGAAAACGAACTGTCCGTCGTAGAAGGCATGCAGTTCGACCGCGGCTACCTGTCGCCGTACTTCGTCAACAAGCCGGACACCATGGTTGCCGAGCTGGAAGGCCCgc                                                                                                                        >  1:5029348/1‑150 (MQ=255)

                              tCGGTAAAGAAGGCGTGATCACCGTTGAAGAAGGCTCGGGCCTGGAAAACGAACTGTCCGTCGTAGAAGGCATGCAGTTCGACCGCGGCTACCTGTCGCCGTACTTCGTCAACAAGCCGGACACCATGGTTGCCGAGCTGGAAGGCCCgc                                                                                                                        <  2:2719932/150‑1 (MQ=255)

                               cGGTTAAGAAGGCGTGATCACCGTTGAAGAAGGCTCGGGCCTGGAAAACGAACTGTCCGTCGTAGAAGGCATGCAGTTCGACCGCGGCTACCTGTCGCCGTACTTCGTCAACAAGCCGGACACCATGGTTGCCGAGCTGGAAGGCCCgct                                                                                                                       <  1:4461255/150‑1 (MQ=255)

                                 gTAAAGAAGGCGTGATCACCGTTGAAGAAGGCTCGGGCCTGGAAAACGAACTGTCCGTCGTAGAAGGCATGCAGTTCGACCGCGGCTACCTGTCGCCGTACTTCGTCAACAAGCCGGACACCATGGTTGCCGAGCTGGAAGGCCCgctgc                                                                                                                     >  2:5070327/1‑150 (MQ=255)

                                    aagaagGCGTGATCACCGTTGAAGAAGGCTCGGGCCTGGAAAACGAACTGTCCGTCGTAGAAGGCATGCAGTTCGACCGCGGCTACCTGTCGCCGTACTTCGTCAACAAGCCGGACACCATGGTTGCCGAGCTGGAAGGCCCgctgctgc                                                                                                                  <  1:1042920/150‑1 (MQ=255)

                                         ggCGAGATCCCCGTTGAAGAAGGCTCGGGCCGGGAAAACGAACTGTCCGACGTAGAAGGCAAGCAGTTCGACCGCGGCAACCTGTCGCCGTACTTCGTCAACAAGCCGGACACCATGCTTGCCGAGCTGGAAGGCCCGctgctgctgctg                                                                                                             >  2:412156/1‑150 (MQ=255)

                                               aTCACCGTTGAAGAAGGCTCGGGCCTGGAAAACGAACTGTCCGTCGTAGAAGGCATGCAGTTCGACCGCGGCTACCTGTCGCCGTACTTCGTCAACAAGCCGGACACCATGGTTGCCGAGCTGGAAGGCCCGCTGCTGCTGCTGGTCGAc                                                                                                       >  1:1275492/1‑150 (MQ=255)

                                               aTCACCGTTGAAGAAGGCTCGGGCCTGGAAAACGAACTGTCCGTCGTAGAAGGCATGCAGTTCGACCGCGGCTACCTGTCGCCGGACTTCGTCAACAAGCCGGACACCATGGTTGCCGAGCTGGAAGGCCCGCTGCTGCTGCTGGTCGAc                                                                                                       <  2:411710/150‑1 (MQ=255)

                                                 cACCGTTGAAGAAGGCTCGGGCCTGGAAAACGAACTGTCCGTCGTAGAAGGCATGCAGTTCGACCGCGGCTACCTGTCGCCGTACTTCGTCAACAAGCCGGACACCATGGTTGCCGAGCTGGAAGGCCCGCTGCTGCTGCTGGTCGACaa                                                                                                     >  1:4585825/1‑150 (MQ=255)

                                                  aCCGTTGAAGAAGGCTCGGGCCTGGAAAACGAACTGTCCGTCGTAGAAGGCATGCAGTTCGACCGCGGCTACCTGTCGCCGTACTTCGTCAACAAGCCGGACACCATGGTTGCCGAGCTGGAAGGCCCGCTGCTGCTGCTGGTCGACAAg                                                                                                    >  1:2734522/1‑150 (MQ=255)

                                                   ccGTTGAAGAAGGCTCGGGCCTGGAAAACGAACTGTCCGTCGTAGAAGGCATGCAGTTCGACCGCGGCTACCTGTCGCCGTACTTCGTCAACAAGCCGGACACCATGGTTGCCGAGCTGGAAGGCCCGCTGCTGCTGCTGGTCGACAAGa                                                                                                   >  1:1020613/1‑150 (MQ=255)

                                                   ccGTTGAAGAAGGCTCGGGCCTGGAAAACGAACTGTCCGTCGTAGAAGGCATGCAGTTCGACCGCGGCTACCTGTCGCCGTACTTCGTCAACAAGCCGGACACCATGGTTGCCGAGCTGGAAGGCCCGCTGCTGCTGCTGGTCGACAAGa                                                                                                   <  2:2061347/150‑1 (MQ=255)

                                                     gTTGAAGTAGGCTAGGGCCTGGAAAACGAACTGTCCGTCGTAGAAGTCATGCAGTTCGACCGCGGCTACCTGTCGACGTACTTCGTCATCCAGCCGGACTCCATGGTTGCCGAGCTGGAAGGCCCGCTGCTGCAGCTGGTCGACAAGaaa                                                                                                 <  2:841994/150‑1 (MQ=255)

                                                     gTTGAAGAAGGCTCGGGCCTGGAAAACGAACTGTCCGTCGTAGAAGGCATGCAGTTCGACCGCGGCTACCTGTCGCCGTACTTCGTCAACAAGCCGGACACCATGGTTGCCGAGCTGGAAGGCCCGCTGCTGCTGCTGGTCGACAAGaaa                                                                                                 >  1:509875/1‑150 (MQ=255)

                                                        gaagaaGGCTCGGGCCTGGAAAACGAACTGTCCGTCGTAGAAGGCATGCAGTTCGACCGCGGCTACCTGTCGCCGTACTTCGTCAACAAGCCGGACACCATGGTTGCCGAGCTGGAAGGCCCGCTGCTGCTGCTGGTCGACAAGAAAAtc                                                                                              <  1:877663/150‑1 (MQ=255)

                                                         aagaagGCTCGGGCCTGGAAAACGAACTGTCCGTCGTAGAAGGCATGCAGTTCGACCGCGGCTACCTGTCGCCGTACTTCGTCAACAAGCCGGACACCATGGTTGCCGAGCTGGAAGGCCCGCTGCTGCTGCTGGTCGACACGAAAAtct                                                                                             >  2:1212196/1‑150 (MQ=255)

                                                               gCTCGGGCCTGGAAAACGAACTGTCCGTCGTAGAAGGCATGCAGTTCGACCGCGGCTACCTGTCGCCGTACTTCGTCAACAAGCCGGACACCATGGTTGCCGAGCTGGAAGGCCCGCTGCTGCTGCTGGTCGACAAGAAAATCTCCAACa                                                                                       >  2:135237/1‑150 (MQ=255)

                                                               gCTCGGGCCTGGAAAACGAACTGTCCGTCGTAGAAGGCATGCAGTTCGACCGCGGCTACCTGTCGCCGTACTTCGTCAACAAGCCGGACACCATGGTTGCCGAGCTGGAAGGCCCGCTGCTGCTGCTGGTCGACAAGAAAATCTCCAACa                                                                                       >  2:1517862/1‑150 (MQ=255)

                                                                cTCGGGTCTGGAAAACGAACTGTCCGTCGTAGAAGGCATGCAGTTCGACCGCGGCTACCTGTCGCCGTACTTCGTCAACAAGCCGGACACCATGGTTGCCGAGCTGGAAGGCCCGCTGCTGCTGCTGGTCGACAAGAAAATCTCCAACAt                                                                                      <  2:670751/150‑1 (MQ=255)

                                                                 tCGGGCCTGGAAAACGAACTGTCCGTCGTAGAAGGCATGCAGTTCGACCGCGGCTACCTGTCGCCGTACTTCGTCAACAAGCCGGACACCATGGTTGCCGAGCTGGAAGGCCCGCTGCTGCTGCTGGTCGACAAGAAAATCTCCAACATc                                                                                     <  2:2169622/150‑1 (MQ=255)

                                                                 tCGGGCCTGGAAAACGAACTGTCCGTCGTAGAAGGCATGCAGTTCGACCGCGGCTACCTATCGCCGTACTTCGTCAACAAGCCGGACACCATGGTTGCCGAGCTGGAAGGCCCGCTGCTGCTGCTGGTCGACAAGAAAATCTCCAACATc                                                                                     >  1:2278669/1‑150 (MQ=255)

                                                                  cGGGCCTGGAAAACGAACTGTCCGTCGTAGAAGGCATGCAGTTCGACCGCGGCTACCTGTCGCCGTACTTCGTCAACAAGCCGGACACCATGGTTGCCGAGCTGGAAGGCCCGCTGCTGCTGCTGGTCGACAAGAAAATCTCCAACATcc                                                                                    <  2:1514774/150‑1 (MQ=255)

                                                                  cGGGCCTGGAAAACGAACTGTCCGTCGTAGAAGGCATGCAGTTCGACCGCGGCTACCTGTCGCCGTACTTCGTCAACAAGCCGGACACCATGGTTGCCGAGCTGGAAGGCCCGCTGCTGCTGCTGGTCGACAAGAAAATCTCCAACATcc                                                                                    <  2:2244103/150‑1 (MQ=255)

                                                                    ggCCTGGAAAACGAACTGTCCGTCGTAGAAGGCATGCAGTTCGACCGCGGCTACCTGTCGCCGTACTTCGTCAACAAGCCGGACACCATGGTTGCCGAGCTGGAAGGCCCGCTGCTGCTGCTGGTCGACAAGAAAATCTCCAACATCCGt                                                                                  <  1:2593070/150‑1 (MQ=255)

                                                                        acgAAAACCAACTCTCCGTCCTAGAACGCAACCAGTTCGACCGCGGCTACCTGTCGCCGTACTTCGTCAACAAGCCCGACACCATGCTAGCCGAGCTGGAAGGCCCGCTGCTGCTGCACGTCGACAACAAAATCTCCAACATCCGTGAgc                                                                              <  2:3490645/148‑1 (MQ=255)

                                                                            aaaCGAACTGTCCGTCGTAGAAGGCATGCAGTTCGACCGCGGCTACCTGTCGCCGTACTTCGTCAACAAGCCGGACACCATGGTTGCCGAGCTGGAAGGCCCGCTGCTGCTGCTGGTCGACAAGAAAATCTCCAACATCCGTGAgctgct                                                                          <  1:837723/150‑1 (MQ=255)

                                                                            aaaCGAACTGTCCGTCGTAGAAGGCATGCAGTTCGACCGCGGCTACCTGTCGCCGTACTTCGTCAACAAGCCGGACACCATGGTTGCCGAGCTGGAAGGCCCGCTGCTGCTGCTGGTCGACAAGAAAATCTCCAACATCCGTGAgctgct                                                                          >  2:346314/1‑150 (MQ=255)

                                                                             aacgaacgGTCCGTCGTAGAAGGCATGCAGTTCGACCGCGGCTACCTGTCGCCGTACTTCGTCAACAAGCCGGACACCATGGTTGCCGAGCTGGAAGGCCCGCTGCTGCTGCTGGTCGACAAGAAAATCTCCAACATCCGTGAGctgctg                                                                         <  1:3787619/150‑1 (MQ=255)

                                                                             aaCGAACTGTCCGTCGTAGAAGGCATGCAGTTCGACCGCGGCTACCTGTCGCCGTACTTCGTCAACAAGCCGGACACCATGGTTGCCGAGCTGGAAGGCCCGCTGCTGCTGCTGGTCGACAAGAAAATCTCCAACATCCGTGAGctgctg                                                                         >  1:5181232/1‑150 (MQ=255)

                                                                              aCGAACTGTCCGTCGTAGAAGGCATGCAGTTCGACCGCGGCTACCTGTCGCCGTACTTCGTCAACAAGCCGGACACCATGGTTGCCGAg                                                                                                                                     >  1:1376258/1‑89 (MQ=255)

                                                                              aCGAACTGTCCGTCGTAGAAGGCATGCAGTTCGACCGCGGCTACCTGTCGCCGTACTTCGTCAACAAGCCGGACACCATGGTTGCCGAg                                                                                                                                     <  2:1376258/89‑1 (MQ=255)

                                                                              aCGAACTGTCCGTCGTAGAAGGCATGCAGTTCGACCGCGGCTACCTGTCGCCGTACTTCGTCAACAAGCCGGACACCATGGTTGCCGAGCTGGAAGGCCCGCTGCTGCTGCTGGTCGACAAGAAAATCTCCAACATCCGTGAGCtgctgc                                                                        <  1:3730789/150‑1 (MQ=255)

                                                                              aCGAACTGGCAGTCGTAGAAGGCATGCAGTTCGACCGCGGCTACCTGTCGCCGTACTTCGTCAACAAGCCGGACACCATGGGTGCCGAGCTGGAAGGCCCGCTGCTGCTGCTGGTCGACAAGAAAATCTCCACCATCCGTGAGCtgctgc                                                                        <  1:3730760/150‑1 (MQ=255)

                                                                                  aCTGTCCGTCGTAGAAGGCATGCAGTTCGACCGCGGCTACCTGTCGCCGTACTTCGTCAACAAGCCGGACACCATGGTTGCCGAGCTGGAAGGCCCGctgctgctgctg                                                                                                             >  2:4974126/1‑109 (MQ=255)

                                                                                    tGTCCGTCGTAGAAGGCATGCAGTTCGACCGCGGCTACCTGTCGCCGTACTTCGTCAACAAGCCGGACACCATGGTTGCCGAGCTGGAAGGCCCGCTGCTGCTGCTGGTCGACAAGAAAATCTCCAACATCCGTGAGCTGCTGCCAGTTc                                                                  <  1:4569508/150‑1 (MQ=255)

                                                                                     gTCCGTCGTAGAAGGCATGCAGTTCGACCGCGGCTACCTGTCGCCGTACTTCGTCAACAAGCCGGACACCATGGTTGCCGAGCTGGAAGGCCCGCTGCTGCTGCTGGTCGACAAGAAAATCTCCAACATCCGTGAGCTGCTGCCAGTTCt                                                                 <  1:3978714/150‑1 (MQ=255)

                                                                                     gTCCGTCGTAGAAGGCATGAAGTTCGACCGCGGCTACCTGTCGCCGTACTTCGTCAACAAGCCGGACACCATGGTTGCCGAGCTGGAAGGCCCGCTGCTGCTGCTGGTCGACAAGAAAATCTCCAACATCCGTGAGCTGCTGCCAGTTCt                                                                 <  1:524131/150‑1 (MQ=255)

                                                                                        cgtcgtAGAAGGCATGCAGTTCGACCGCGGCTACCTGTCGCCGTACTTCGTCAACAAGCCGGACACCATGGTTGCCGAGCTGGAAGGCCCGCTGCTGCTGCTGGTCGAc                                                                                                       >  1:2213476/1‑109 (MQ=255)

                                                                                         gtcgtAGAAGGCATGCAGTTCGACCGCGGCTACCTGTCGCCGTACTTCGTCAACAAGCCGGACACCATGGTTGCCGAGCTGGAAGGCCCGCTGCTGCTGCTGGTCGACAAGAAAATCTCCAACATCCGTGAGCTGCTGCCAGTTCTGGaa                                                             <  1:472586/150‑1 (MQ=255)

                                                                                           cgtAGAAGGCATGCAGTTCGACCGCGGCTACCTGTCGCCGTACTTCGTCAACAAGCCGGACACCATGGTTGCCGAGCTGGAAGGCCCGCTGCTGCTGCTGGTCGACAAGAAAATCTCCAACATCCGTGAGCTGCTGCCAGTTCTGGAAGc                                                           <  2:2956017/150‑1 (MQ=255)

                                                                                             tAGAAGGCATGCAGTTCGACCGCGGCTACCTGTCGCCGTACTTCGTCAACAAGCCGGACACCATGGTTGCCGAGCTGGAAGGCCCGCTGCTGCTGCTGGTCGACAAGAAAATCTCCAACATCCGTGAGCTGCTGCCAGTTCTGGAAGCCg                                                         >  2:5172331/1‑150 (MQ=255)

                                                                                             tAGAAGGCATGCAGTTCGACCGCGGCTACCTGTCGCCGTACTTCGTCAACAAGCCGGACACCATGGTTGCCGAGCTGGAAGGCCCGCTGCTGCTGCTGGTCGACAAGAAAATCTCCAACATCCGTGAGCTGCTGCCAGTTCTGGAAGCCg                                                         >  2:1991931/1‑150 (MQ=255)

                                                                                                aaGGCATGCAGTTCGACCGCGGCTACCTGTCGCCGTACTTCGTCAACAAGCCGGACACCATGGTTGCCGAGCTGGAAGGCCCGCTGCTGCTGCTGGTCGACAAGAAAATCTCCAACATCCGTGAGCTGCTGCCAGTTCTGGAAGCCGTTg                                                      <  2:2972965/150‑1 (MQ=255)

                                                                                                 aGGCATGCAGTTCGACCGCGGCTACCTGTCGCCGTACTTCGTCAACAAGCCGGACACCATGGTTGCCGAGCTGGAAGGCCCGCTGCTGCTGCTGGTCGACAAGAAAATCTCCAACATCCGTGAGCTGCTGCCAGTTCTGGAAGCCGTTGc                                                     >  1:2216835/1‑150 (MQ=255)

                                                                                                   gCATGCAGTTCGACCGCGGCTACCTGTCGCCGTACTTCGTCAACAAGCCGGACACCATGGTTGCCGAGCTGGAAGGCCCGCTGCTGCTGCTGGTCGACAAGAAAATCTCCAACATCCGTGAGCTGCTGCCAGTTCTGGAAGCCGTTGCCa                                                   >  2:466020/1‑150 (MQ=255)

                                                                                                     aTGCAGTTCGACCGCGGCTACCTGTCGCCGTACTTCGTCAACAAGCCGGACACCATg                                                                                                                                              >  1:2180961/1‑57 (MQ=255)

                                                                                                     aTGCAGTTCGACCGCGGCTACCTGTCGCCGTACTTCGTCAACAAGCCGGACACCATg                                                                                                                                              <  2:2180961/57‑1 (MQ=255)

                                                                                                     aTGCAGTTCGACCGCGGCTACCTGTCGCCGTACTTCGTCAACAAGCCGGACACCATGGTTGCCGAGCTGGAAGGCCCGCTGCTGCTGCTGGTCGACAAGAAAATCTCCAACATCCGTGAGCTGCTGCCAGTTCTGGAAGCCGTTGCCAAg                                                 <  1:4628122/150‑1 (MQ=255)

                                                                                                           ttCGACCGCGGCTACCTGTCGCCGTACTTCGTCAACAAGCCGGACACCATGGTTGCCGAGCTGGAAGGCCCGCTGCTGCTGCTGGTCGACAAGAAAATCTCCAACATCCGTGAGCTGCTGCCAGTTCTGGAAGCCGTTGCCAAggccggc                                           <  2:2512778/150‑1 (MQ=255)

                                                                                                           ttCGACCGCGGCTACCTGTCGCCGTACTTCGTCAACAAGCCGGACACCATGGTTGCCGAGCTGGAAGGCCCGCTGCTGCTGCTGGTCGACAAGAAAATCTCCAACATCCGTGAGCTGCTGCCAGTTCTGGAAGCCGTTGCCAAggccggc                                           >  2:5431162/1‑150 (MQ=255)

                                                                                                                ccGCGGCTACCTGTCGCCGTACTTCGTCAACAAGCCGGACACCATGGTTGCCGAGCTGGAAGGCCCGCTGCTGCTGCTGGTCGACAAGAAAATCTCCAACATCCGTGAGCTGCTGCCAGTTCTGGAAGCCGTTGCCAAGGCCGGCCGccc                                      >  1:3696362/1‑150 (MQ=255)

                                                                                                                 cgcgGCTACCTGTCGCCGTACTTCGTCAACAAGCCGGACACCATGGTTGCCGAGCTGGTAGGCCCGCTGCTGCTGCTGGTCGCCAAGAAAATCTCCAACATCCGTGAGCTGCTGCCAGTTCTGGAAGCCGGTGCCAGGGCCGgccgcgcg                                     >  2:4771114/1‑147 (MQ=255)

                                                                                                                     gCTACCTGTCGCCGTACTTCGTCAACAAGCCGGACACCATGGTTGCCGAGCTGGAAGGCCCGCTGCTGCTGCTGGTCGACAAGAAAATCTCCAACATCCGTGAGCTGCTGCCAGTTCTGGAAGCCGTTGCCAAGGCCGGCCGCCCActgc                                 <  2:1405263/150‑1 (MQ=255)

                                                                                                                               gCCGTACTTCGTCAACAAGCCGGACACCATGGTTGCCGAGCTGGAAGGCCCGCTGCTGCTGCTGGTCGACAAGAAAATCTCCAACATCCGTGAGCTGCTGCCAGTTCTGGAAGCCGTTGCCAAGGCCGGCCGCCCACTGCTGATCGTTGc                       <  1:4217718/150‑1 (MQ=255)

                                                                                                                               gCCGTACTTCGTCAACAAGCCGGACACCATGGTTGCCGAGCTGGAAGGCCCGCTGCTGCTGCTGGTCGACAAGAAAATCTCCAACATCCGTGAGCTGCTGCCAGTTCTGGAAGCCGTTGCCAAGGCCGGCCGCCCACTGCTGATCGTTGc                       <  1:2010047/150‑1 (MQ=255)

                                                                                                                                ccGTACTTCGTCAACAAGCCGGACACCATGGTTGCCGAGCTGGAAGGCCCGCTGCTGCTGCTGGTCGACAAGAAAATCTCCAACATCCGTGAGCTGCTGCCAGTTCTGGAAGCCGTTGCCAAGGCCGGCCGCCCACTGCTGATCGTTGcc                      >  2:401866/1‑150 (MQ=255)

                                                                                                                                ccGTACTTCGTCAACAAGCCGGACACCATGGTTGCCGAGCTGGAAGGCCCGCTGCTGCTGCTGGTCGACAAGAAAATCTCCAACATCCGTGAGCTGCTGCCAGTTCTGGAAGCCGTTGCCAAGGCCGGCCGCCCACTGCTGATCGTTGcc                      >  2:3149089/1‑150 (MQ=255)

                                                                                                                                     cTTCGTCAACAAGCCGGACACCATGGTTGCCGAGCTGGAAGGCCCGCTGCTGCTGCTGGTCGACAAGAAACTCTCCAACATCCGTGAGCTGCTGCCAGTTCTGGAAGCCGTTGCCAAGGCCGGCCGCCCCCTGCTGATCGTTGCCGAAGa                 >  2:296867/1‑150 (MQ=255)

                                                                                                                                     cTTCGTCAACAAGCCGGACACCATGGTTGCCGAGCTGGAAGGCCCGCTGCTGCTGCTGGTCGACAAGAAAATCTCCAACATCCGTGAGCTGCTGCCAGTTCTGGAAGCCGTTGCCAAGGCCGGCCGCCCACTGCTGATCGTTGCCGAAGa                 >  1:274088/1‑150 (MQ=255)

                                                                                                                                     cTTCGTCAACAAGCCGGACACCATGGTTGCCGAGCTGGAAGGCCCGCTGCTGCTGCTGGTCGACAAGAAAATCTCCAACATCCGTGAGCTGCTGCCAGTTCTGGAAGCCGTTGCCAAGGCCGGCCGCCCACTGCTGATCGTTGCCGAAGa                 >  1:835797/1‑150 (MQ=255)

                                                                                                                                        cGTCAACAAGCCGGACACCATGGTTGCCGAGCTGGAAGGCCCGCTGCTGCTGCTGGTCGACAAGAAAATCTCCAACATCCGTGAGCTGCTGCCAGTTCTGGAAGCCGTTGCCAAGGCCGGCCGCCCACTGCTGATCGTTGCCGAAGACGt              >  2:363266/1‑150 (MQ=255)

                                                                                                                                         gTCAACAAGCCGGACACCATGGTTGCCGAGCTGGAAGGCCCGCTGCTGCTGCTGGTCGACAAGAAAATCTCCAACATCCGTGAGCTGCTGCCAGTTCTGGAAGCCGTTGCCAAGGCCGGCCGCCCACTGCTGATCGTTGCCGAAGACGtt             <  2:839711/150‑1 (MQ=255)

                                                                                                                                             acaaGCCGGACACCATGGTTGCCGAGCTGGAAGGCCCGCTGCTGCTGCTGGTCGACAAGAAAATCTCCAACATCCGTGAGCTGCTGCCAGTTCTGGAAGCCGTTGCCAAGGCCGGCCGCCCACTGCTGATCGTTGCCGAAGACGtt             >  2:1909917/1‑146 (MQ=255)

                                                                                                                                             acaaGCCGGACACCATGGTTGCCGAGCTGGAAGGCCCGCTGCTGCTGCTGGTCGACAAGAAAATCTCCAACATCCGTGAGCTGCTGCCAGTTCTGGAAGCCGTTGCCAAGGCCGGCCGCCCACTGCTGATCGTTGCCGAAGACGtt             <  1:1909917/146‑1 (MQ=255)

                                                                                                                                                 gCCGGACACCATGGTTGCCGAGCTGGAAGGCCCGCTGCTGCTGCTGGTCGACAAGAAAATCTCCAACATCCGTGAGCTGCTGCCAGTTCTGGAAGCCGTTGCCAAGGCCGGCCGCCCACTGCTGATCGTTGCCGAAGAc                <  1:1074259/139‑1 (MQ=255)

                                                                                                                                                  ccGGACACCATGGTTGCCGAGCTGGAAGGCCCGCTGCTGCTGCTGGTCGACAAGAAAATCTCCAACATCCGTGAGCTGCTGCCAGTTCTGGAAGCCGTTGCCAAGGCCGGCCGCCCACTGCTGATCGTTGCCGAAGACGTTGAAGGTGaa    <  2:4543638/150‑1 (MQ=255)

                                                                                                                                                  ccGGACACCATGGTTGCCGAGCTGGAAGGCCCGCTGCTGCTGCTGGTCGACAAGAAAATCTCCAACATCCGTGAGCTGCTGCCAGTTCTGGAAGCCGTTGCCAAGGCCGGCCGCCCACTGCTGATCGTTGCCGAAGACGTTGAAGGTGaa    <  2:4543610/150‑1 (MQ=255)

                                                                                                                                                  ccGGACACCATGGTTGCCGAGCTGGAAGGCCCGCTGCTGCTGCTGGTCGACAAGAAAATCTCCAACATCCGTGAGCTGCTGCCAGTTCTGGAAGCCGTTGCCAAGGCCGGCCGCCCACTGCTGATCGTTGCCGAAGACGTTGAAGGTGaa    <  2:2278669/150‑1 (MQ=255)

                                                                                                                                                  ccGGACACCAGGGGTGCCGAGCTGGAAGGCCCGCTGCTGCTGCTGGTCGACAAGAAAATCTCCAACCTCCGTGAGCTGCTGCCAGTTCTGGAAGCCGTTGCCAAGGCCGGCCGCCCACTGCTGATCGTTGCCGAAGACGTTGAAGGTGaa    <  2:463132/150‑1 (MQ=255)

                                                                                                                                                    ggACACCATGGTTGCCGAGCTGGAAGGCCCGCTGCTGCTGCTGGTCGACAAGAAAATCTCCAACATCCGTGAGCTGCTGCCAGTTCTGGAAGCCGTTGCCAAGGCCGGCCGCCCACTGCTGATCGTTGCCGAAGACGTTGAAGGTGAAgc  >  2:2624043/1‑150 (MQ=255)

GTGAAATCATCGCCGAAGCCATGGAAAAAGTCGGTAAAGAAGGCGTGATCACCGTTGAAGAAGGCTCGGGCCTGGAAAACGAACTGTCCGTCGTAGAAGGCATGCAGTTCGACCGCGGCTACCTGTCGCCGTACTTCGTCAACAAGCCGGACACCATGGTTGCCGAGCTGGAAGGCCCGCTGCTGCTGCTGGTCGACAAGAAAATCTCCAACATCCGTGAGCTGCTGCCAGTTCTGGAAGCCGTTGCCAAGGCCGGCCGCCCACTGCTGATCGTTGCCGAAGACGTTGAAGGTGAAGC  >  NC_002947/1550118‑1550415

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 11 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`