Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 1,615,730 C→T 7.1% A22T (GCC→ACC)  PP_1415 ← membrane protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029471,615,7300CT 7.1% 395.7 / 13.3 142A22T (GCC→ACC) PP_1415membrane protein
Reads supporting (aligned to +/- strand):  ref base C (84/40);  new base T (4/6);  total (88/54)
Fisher's exact test for biased strand distribution p-value = 9.14e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 144 total aligned reads displayed.

CAGGCCAATACCGATGCCGATGATCCACTGCCCGCACTTGCGGCCGTTGGGGATTTCCGAAAGCTGCCAACGCGTCAGGCAGCGCACCAGGATGATCGCCAGCAATGAACCGACCATCCATGGCAAAGGCCAGCCGACCTTGCTGGCGGCAAAACCGCCAGCAAGGCCGACCAGCCCGGTCGCGACGAACAATGGCAACGACCGATCAGGCATCGGCCAGGGCCCGGCGCTGCAGGCTGCGTTTGCGCCAGATGCGCAGCAGCGGCAGGGCGAGCATTACCACTACCAGCGCCCA  >  NC_002947/1615581‑1615875
                                                                                                                                                     |                                                                                                                                                 
     cAATACCGATGCCGATGATCCACTGCCCGCACTTGCGGCCGTTGGGGATTTCCGAAAGCTGCCAACGCGTCAGGCAGCGCACCAGGATGATCGCCAGCAATGAACCGACCATCCATGGCAAAGGCCAGCCGACCTTGCTGGCGGCAAAAc                                                                                                                                              >  2:247242/1‑150 (MQ=255)
        tACCGATGCCGATGATCCACTGCCCGCACTTGCGGCCGTTGGGGATTTCCGAAAGCTGCCAACGCGTCAGGCAGCGCACCAGGATGATCGCCAGCAATGAACCGACCATCCATGGCAAAGGCCAGCCGACCTTGCTGGCGGCAAAACCGc                                                                                                                                           <  1:4242457/150‑1 (MQ=255)
         aCCGATGCCGATGATCCACTGCCCGCACTTGCGGCCGTTGGGGATTTCCGAAAGCTGCCAACGCGTCAGGCAGCGCACCAGGATGATCGCCAGCAATGAACCGACCATCCATGGCAAAGGCCAGCCGACCTTGCTGGCGGCAAAACCGcc                                                                                                                                          <  1:4871624/150‑1 (MQ=255)
          ccgatgccgatgATCCACTGCCCGCACTTGCGGCCGTTGGGGATTTCCGAAAGCTGCCAACGCGTCAGGCAGCGCACCAGGATGATCGCCAGCAATGAACCGACCATCCATGGCAAAGGCCAGCCGACCTTGCTGGCGGCAAAACCGCCa                                                                                                                                         <  1:4379411/150‑1 (MQ=255)
              tgccgatgATCCACTGCCCGCACTTGCGGCCGTTGGGGATTTCCGAAAGCTGCCAACGCGTCAGGCAGCGCACCAGGATGATCGCCAGCAATGAACCGACCATCCATGGCAAAGGCCAGCCGACCTTGCTGGCGGCAAAACCGCCAGCaa                                                                                                                                     <  2:1254647/150‑1 (MQ=255)
              tgccgatgATCCACTGCCCGCACTTGCGGCCGTTGGGGATTTCCGAAAGCTGCCAACGCGTCAGGCAGCGCACCAGGATGATCGCCAGCAATGAACCGACCATCCATGGCAAAGGCCAGCCGACCTTGCTGGCGGCAAAACCGCCAGCaa                                                                                                                                     <  2:14847/150‑1 (MQ=255)
                 cgatgATCCACTGCCCGCACTTGCGGCCGTTGGGGATTTCCGAAAGCTGCCAACGCGTCAGGCAGCGCACCAGGATGATCGCCAGCAATGAACCGACCATCCATGGCAAAGGCCAGCCGACCTTGCTGGCGGCAAAACCGCCAGCAAGGc                                                                                                                                  <  2:109588/150‑1 (MQ=255)
                  gatgatCCACTGCCCGCACTTGCGGCCGTTGGGGATTTCCGAAAGCTGCCAACGCGTCAGGCCGCGCACCAGGATGATCGCCAGCAATGAACCGACCATCCATGGCAAAGGCCAGCCGACCTTGCTGGCGGCCAAACCGCCAGCAAGGcc                                                                                                                                 >  1:3660809/1‑150 (MQ=255)
                  gatgatCCACTGCCCGCACTTGCGGCCGTTGGGGATTTCCGAAAGCTGCCAACGCGTCAGGCAGCGCACCAGGATGATCGCCAGCAATGAACCGACCATCCATGGCAAAGGCCAGCCGACCTTGCTGGCGGCAAAAACGCCAGCAAGGcc                                                                                                                                 >  1:1062369/1‑150 (MQ=255)
                  gatgatCCACTGCCCGCACTCGCGGCCGTTGGGGATTTCCGAAAGCTGCCAACGCGTCAGGCAGCGCACCAGGATGATCGCCAGCAATGAACCGACCATCCATGGCAAAGGCCAGCCGACCTTGCTGGCGGTTTTGCCGCCAGCAAggtc                                                                                                                                 <  2:2983229/150‑3 (MQ=255)
                  gatgatCCACTGCCCGCACTCGCGGCCGTTGGGGATTTCCGAAAGCTGCCAACGCGTCAGGCAGCGCACCACGATGATCGCCAGCAATGAACCGACCATCCATGGCAAAGGCCAGCCGACCTTCCTGGCGGTTTTGCCGCCAGCAAggtc                                                                                                                                 <  2:3466125/150‑3 (MQ=255)
                   atgatCCACTGCCCGCACTTGCGGCCGTTGGGGATTTCCGAAAGCTGCCAACGCGTCAGGCAGCGCACCAGGATGATCGCCAGCAATGAACCGACCATCCATGGCAAAGGCCAGCCGACCTTGCTGGCGGTTTTGCCGCCAGCAAGGTCg                                                                                                                                <  1:358972/150‑1 (MQ=255)
                        ccACTGCCCGCACTTGCGGCCGTTGGGGATTTCCGAAAGCTGCCAACGCGTCAGGCAGCGCACCAGGATGATCGCCAGCAATGAACCGACCATCCATGGCAAAGGCCAGCCGACCTTGCTGGCGGCAAAACCGCCCGCAAGGCCGACCAg                                                                                                                           >  2:4702964/1‑150 (MQ=255)
                          aCTGCCCGCACTTGCGGCCGTTGGGGATTTCCGAAAGCTGCCAACGCGTCAGGCAGCGCACCAGGATGCTCGCCAGCAATGAACCGACCATCCATGGCAAAGGCCAGCCGACCTTGCTGGCGGCAAAACCGCCAGCAAGGCCGACCAGcc                                                                                                                         >  2:4699540/1‑150 (MQ=255)
                          aCTGCCCGCACTTGCGGCCGTTGGGGATTTCCGAAAGCTGCCAACGCGTCAGGCAGCGCACCAGGATGATCGCCAGCAATGAACCGACCATCCATGGCAAAGGCCAGCCGACCTTGCTGGCGGCAAAACCGc                                                                                                                                           >  1:5102122/1‑132 (MQ=255)
                          aCTGCCCGCACTTGCGGCCGTTGGGGATTTCCGAAAGCTGCCAACGCGTCAGGCAGCGCACCAGGATGATCGCCAGCAATGAACCGACCATCCATGGCAAAGGCCAGCCGACCTTGCTGGCGGCAAAACCGc                                                                                                                                           <  2:5102122/132‑1 (MQ=255)
                          aCTGCCCGCACTTGCGGCCGTTGGGGATTTCCGAAAGCTGCCAACGCGTCAGGCAGCGCACCAGGATGATCGCCAGCAATGAACCGACCATCCATGGCAAAGGCCAGCCGACCTTGCTGGCGGCAAAACCGCCAGCAAGGCCGACCAGcc                                                                                                                         >  2:5172368/1‑150 (MQ=255)
                              cccGCACTTGCGGCCGTTGGGGATTTCCGAAAGCTGCCAACGCGTCAGGCAGCGCACCAGGATGATCGCCAGCAATGAACCGACCATCCATGGCAAAGGCCAGCCGACCTTGCTGGCGGCAAAACCGCCAGCAAGGCCGACCAGCCCggg                                                                                                                     >  2:4346738/1‑149 (MQ=255)
                              cccGCACTTGCGGCCGTTGGGGATTTCCGAAAGCTGCCAACGCGTCAGGCAGCGCACCAGGATGATCGCCAGCAATGAACCGACCATCCATGGCAAAGGCCAGCCGACCTTGCTGGCGGCAAAACCGCCAGCAAGGCCGACCAGCCCGGt                                                                                                                     >  2:4346616/1‑150 (MQ=255)
                                cGCACTTGCGGCCGTTGGGGATTTCCGAAAGCTGCCAACGCGTCAGGCAGCGCACCAGGATGATCGCCAGCAATGAACCGACCATCCATGGCAAAGGCCAGCCGACCTTGCTGGCGGCAAAACCGCCAGCAACGCCGACCCGCCCGGTcg                                                                                                                   >  1:5174209/1‑150 (MQ=255)
                                 gCACTTGCGGCCGTTGGGGATTTCCGAAAGCTGCCAACGCGTCAGGCAGCGCACCAGGATGATCGCCAGCAATGAACCGACCATCCATGGCAAAGGCCAGCCGACCTTGCTGGCGGCAAAACCCCCAGCAAGGCCGACCAGCCCGGTcgc                                                                                                                  >  1:2327572/1‑150 (MQ=255)
                                  cACTTGCGGCCGTTGGGGATTTCCGAAAGCTGCCAACGCGTCAGGCAGCGCACCAGGATGATCGCCAGCAATGAACCGACCATCCATGGCAAAGGCCAGCCGACCTTGCTGGCGGCAAAACCGCCAGCAAGGCCCACCAGCCCCGTcgcg                                                                                                                 >  1:3694508/1‑150 (MQ=255)
                                  cACTTGCGGCCGTTGGGGATTTCCGAAAGCTGCCAACGCGTCAGGCAGCGCACCAGGATGATCGCCAGCAATGAACCGACCATCCATGGCAAAGGCCAGCCGACCATGCAGGCGGCAAAACCCCCACCAAAGCCGACCACCCCCGgcgcc                                                                                                                 >  1:952556/1‑149 (MQ=255)
                                   aCTTGCGGCCGTTGGGGATTTCCGAAAGCTGCCAACGCGTCAGGCAGCGCACCAGGATGATCGCCAGCAATGAACCGACCATCCATGGCAAAGGCCAGCCGACCTTGCTGGCGGCAAAACCGCCAGCAAGGCCGACCAGCCCGGTCGcga                                                                                                                >  2:2735614/1‑150 (MQ=255)
                                   aCTTGCGGCCGTTGGGGATTTCCGAAAGCTGCCAACGCGTCAGGCAGCGCACCAGGATGATCGCCAGCAATGAACCGACCATCCATGGCAAAGGCCAGCCGACCTTGCTGGCGGCAAAACCGCCAGCAAGGCCGACCAGCCCGGTCGcga                                                                                                                <  2:3583048/150‑1 (MQ=255)
                                   aCTTGCGGCCGTTGGGGATTTCCGAAAGCTGCCAACGCGTCAGGCAGCGCACCAGGATGATCGCCAGCAATGAACCGACCATCCATGGCAAAGGCCAGCCGACCTTGCTGGCGGCAAAACCGCCAGCAAGGCCGACCAGCCCGGTCGcga                                                                                                                <  2:5346251/150‑1 (MQ=255)
                                    cTTGCGGCCGTTGGGGATTTCCGAAAGCTGCCAACGCGTCAGGCAGCGCACCAGGATGATCGCCAGCAATGAACCGACCATCCATGGCAAAGGCCAGCCGACCTTGCTGGCGGCAAAACCGc                                                                                                                                           <  2:5133180/122‑1 (MQ=255)
                                     ttGCGGCCGTTGGGGATTTCCGAAAGCTGCCAACGCGTCAGGCAGCGCACCAGGATGATCGCCAGCAATGAACCGACCATCCATGGCAAAGGCCAGCCGACCATGCATGCGGCAAAAACCCCAGCAAAGCCCACCACCCCCGTCGcgacc                                                                                                              >  1:2436033/1‑149 (MQ=255)
                                          gCCGTTGGGGTTTTCCGAAAGCTGCCAACGCGTCAGGCAGCGCACCAGGATGATCGCCAGCAATTAACCGACCATCCATGGCAACGGCCAGCCGACCTTGCTGGCGGCAAAACCGCCAGCAAGGCCGACCAGCCCGGTCGCGACGAACaa                                                                                                         <  2:3472197/150‑1 (MQ=255)
                                          gCCGTTGGGGATTTCCGAAAGCTGCCAACGCGTCAGGCAGCGCACCAGGATGATCGCCAGCAATGAACCGACCATCCATGGCAAAGGCCAGCCGACCGTGCTGGCGGCCAAACCCCCCGCAAGGCCGACCAGCCCGGGCGCGACGAACaa                                                                                                         >  1:912480/1‑150 (MQ=255)
                                           cggTTGGGTATTTCCGAACGCTGCAAACGCGACTGGCAGCGCACCAGGATGACCGCCAGAAATGACCCCACCATCATTGGCAACGGCCAGCCGACTTTGGTGGGGGAAAACCCGCCAGCAAGGCCGACCAGCCCGGTCGCGACGAACAAt                                                                                                        <  1:4417656/148‑1 (MQ=255)
                                            cGTTGGGTATTTCCTAAAGCTGCCAACGCGTCAGGCAGCGCACCAGGATGAACTCAATCAATTAACCCACCATCAATGGCAACGCCCAGCCTACCTTGCTGGCGGAAAAACCCACACCAAGCCCGACCAGCCCGGTCGCGACGAACAAtc                                                                                                       <  1:4546664/150‑2 (MQ=255)
                                              ttGGGGATTTCCGAAAGCTGCCAACGCGTCAGGCAGCGCACCAGGATGATCGCCAGCAATGAACCGACCATCCATGGCAAAGGCCAGCCGACCTTGCTGGCGGCAAAACCGCCAGCAAGGCCGACCAGCCCGGTCGCGACGAACAATGGc                                                                                                     >  2:3179638/1‑150 (MQ=255)
                                                  ggATTTCCGAAAGCTGCCAACGCGTCAGGCAGCGCACCAGGATGATCGCCAGCAATGAACCGACCATCCATGGCAAAGGCCAGCCGACCTTGCTGGCGGCAAAACCGCCAGCAAGGCCGACCAGCCCGGTCGCGACGAACAATGGCAACg                                                                                                 >  2:2121192/1‑150 (MQ=255)
                                                  ggATTTCCGAAAGCTGCCAACGCGTCAGGCAGCGCACCAGGATGATCGCCAGCAATGAACCGACCATCCATGGCAAAGGCCAGCCGACCTTGCTGGCGGCAAAACCGCCAGCAAGGCCGACCAGCCCGGTCGCGACGAACAATGGCAACg                                                                                                 >  1:4233092/1‑150 (MQ=255)
                                                   gATTTCCGAACGCTGCCAACGCGTCAGGCAGCGCACCAGGATGATCGCCAGCAATGAACCGACCATCCATGGCAACGGCCAGCCGACGTTGCTGGCGGCAAACCCGCCAGAAAGGCCGACCAGCCCGGTCGCGACGAACAATGGCAACGa                                                                                                <  2:2077654/150‑1 (MQ=255)
                                                   gATTTCCGAAAGCTGCCAACGCGTCAGGCAGCGCACCAGGATGATCGCCAGCAATGAACCGACCATCCATGGCAAAGGCCAGCCGACCTTGCTGGCGGCAAAACCGCCAGCAAGGCCGACCAGCCCGGTCGCGACGAACAAAGGCAACGa                                                                                                >  1:604540/1‑150 (MQ=255)
                                                      ttCCGAAAGCTGCCAACGCGTCAGGCAGCGCACCAGGATGATCGCCAGCAATGAACCGACCATCCATGGCAAAGGCCAGCCGACCTTGCTGGCGGCAAAACCGCCAGCAAGGCCGACCAGCCCGGTCGCGACGAACAATGGCAACGACCg                                                                                             >  2:5278865/1‑150 (MQ=255)
                                                      ttCCGAAAGCTGCCAACGCGTCAGGCAGCGCACCAGGATGATCGCCAGCAATGAACCGACCATCCATGGCAAAGGCCAGCCGACCTTGCTGGCGGCAAAACCGCCAGCAAGGCCGACCAGCCCGGTCGCGACGAACAATGGCAACGACCg                                                                                             >  1:1152207/1‑150 (MQ=255)
                                                      ttCCGAAAGCTGCCAACGCGTCAGGCAGCGCACCAGGATGATCGCCAGCAATGAACCGACCATCCATGGCAAAGGCCAGCCGACCTTGCTGGCGGCAAAACCGCCAGCAAGGCCGACCAGCCCGGTCGCGACGAACAATGGCAACGACCg                                                                                             >  2:3386581/1‑150 (MQ=255)
                                                        ccGAAAGCTGCCAACGCGTCAGGCAGCGCACCAGGATGATCGCCAGCAATGAACCGACCATCCATGGCAAAGGCCAGCCGACCTTGCTGGCGGCAAAACCGCCAGCAAGGCCGACCAGCCCGGTCGCGACGAACAATGGCAACGACCGAt                                                                                           >  1:2093839/1‑150 (MQ=255)
                                                         cGAAAGCTGCCAACGCGTCAGGCAGCGCACCAGGATGATCGCCAGCAATGAACCGACCATCCATGGCAAAGGCCAGCCGACCTTGCTGGCGGCAAAACCGCCAGCAAGGCCGACCAGCCCGGTCGCGACGAACAATGGCAACGACCGATc                                                                                          >  1:1201161/1‑150 (MQ=255)
                                                         cGAAAGCTGCCAACGCGTCAGGCAGCGCACCAGGATGATCGCCAGCAATGAACCGACCATCCATGGCAAAGGCCAGCCGACCTTGCTGGCGGCAAAACCGCCAGCAAGGCCGACCAGCCCGGTCGCGACGAACAATGGCAACGACCGATc                                                                                          >  2:3545165/1‑150 (MQ=255)
                                                             aGCTGCCAACGCGTCAGGCAGCGCACCAGGATGATCGCCAGCAATGAACCGACCATCCATGGCAAAGGCCAGCCGACCTTGCTGGCGGCAAAAACGCCAGCAAGGCCGACCACCACGGTCGCCACCAACAATGGCAACGACCGAACAGGc                                                                                      >  1:3294335/1‑150 (MQ=255)
                                                                  ccAACGCGTCAGGCAGCGCACCAGGATGATCGCCAGCAATGAACCGACCATCCATGGCAAAGGCCAGCCGACCTTGCTGGCGGCAAAACCGCCAGCAAGGCCGACCAGCCCGGTCGCGACGAACAATGGCAACGACCGATCAGGCATCgg                                                                                 >  2:3216505/1‑150 (MQ=255)
                                                                   cAACGCGTCAGGCAGCGCACCAGGATGATCGCCAGCAATGAACCGACCATCCATGGCAAAGGCCAGCCGACCTTGCTGGCGGCAAAACCGCCAGCAAGGCCGACCAGCCCGGTCGCGACGAACAAAGGCAACGACCGATCAGGCATCGGc                                                                                >  1:5233280/1‑150 (MQ=255)
                                                                     aCGCGTCTGGCAGCGCACCAGGATGATCGCCAGCAATGAACCGACCATCCATGGCAACGGCCAGCCGACCTTGCTGGCGGCAAAACCGCCAGCAAGGCCGACCAGCCCGGTCGCGACGAACAATGGCAACGACCGATCAGGCATCGGCCa                                                                              <  1:4963548/150‑1 (MQ=255)
                                                                       gcgTCAGGCAGCGCACCAGGATGATCGCCAGCAATGAACCGACCATCCATGGCAAAGGCCAGCCGACCTTGCTGGCGGCAAAACCGCCAGCAAGGCCGACCAGCCCGGTCGCGACGAACAATGGCAACGACCGATCAGGCATCGGCCAgg                                                                            >  2:4566020/1‑150 (MQ=255)
                                                                            aGGCCGCGCACCAGGATGATCGCCAGCAATGAACCGACCCTCCATGGCAAAGGCCAGCCGACCTTGCTGGCGGCAACACCGCCAGCAAGGCCGACCCGCCCGGGCGCGACGAACAATGGCCCCGACCGCTCAGGCCTCGGCCAGGGCCCg                                                                       >  2:3257734/1‑150 (MQ=255)
                                                                              gCAGCGCACCAGGATGATCGCCAGCAATGAACCGACCATCCATGGCAAAGGCCAGCCGACCTTGCTGGCGGCAAAACCGCCCGCAAGGCCGACCCGCCCGGTCGCGCCGAACAATGGCAACGACCGCTCAGGCATCGGCCCGGGCCCGgc                                                                     >  1:304215/1‑150 (MQ=255)
                                                                              gCAGCGCACCAGGATGATCGCCAGCAATGAACCGACCATCCATGGCAAAGGCCAGCCGACCTTGCTGGCGGCAAAACCGCCAGCAAGGCCGACCAGCCCGGTCGCGACGAACAATGGCAACGACCGATCAGGCATCGGCCAGGGCCCGgc                                                                     >  2:4845529/1‑150 (MQ=255)
                                                                                aGCGCACCAGGATGATCGCCAGCAATGAACCGACCATCCATGGCAAAGGCCAGCCGACCTTGCTGGCGGCCAAAACGCCCGCAAGGCCGACCAGCCCGGTCGCGACGAACAATGGCAACGACCCATCAGGCATCGGCCAGGGCCCGgcgc                                                                   >  1:630732/1‑150 (MQ=255)
                                                                                aGCGCACCAGGATGATCGCCAGCAATGAACCGACCATCCATGGCAAAGGCCAGCCGACCTTGCTGGCGGCAAAACCGCCAGCAAgg                                                                                                                                   >  1:749990/1‑86 (MQ=255)
                                                                                aGCGCACCAGGATGATCGCCAGCAATGAACCGACCATCCATGGCAAAGGCCAGCCGACCTTGCTGGCGGCAAAACCGCCAGCAAgg                                                                                                                                   <  2:749990/86‑1 (MQ=255)
                                                                                     aCCAGGATGATCGCCAGCAATGAACCGACCATCCATGGCAAAGGCCAGCCGACCTTGCTGGCGGCAAAACCGCCAGCAAGGCCGACCAGCCCGGTCGCGACGAACAATGGCAACGACCGATCAGGCATCGGCCAGGGCCCGGCGCTGCAg                                                              >  2:3567852/1‑150 (MQ=255)
                                                                                          gatgatCGCCAGCAATGACCCGACCAGCCATGGCAAAGGCCAGCCGACCTGGCTGGCGGCAAAACCGCCAGCAAGGCCGACCAGCCCGGTCGCGACGAACAATGGGAACGACCGATCAGGCATCGGCCAGGGCCCGGCGCTGCAGGCTGc                                                         <  2:3864852/150‑1 (MQ=255)
                                                                                          gatgatCGCCAGCAATGAACCGACCATCCATGGCAAAGGCCAGCCGACCTTGCTGGCGGCAAAACCGCCAGCAAGGCCGACCAGCCCGGTCGCGACGAACAATGGCAACGACCGATCAGGCATCGGCCAGGGCCCGGCGCTGCAGGCTGc                                                         >  1:809108/1‑150 (MQ=255)
                                                                                          gatgatCGCCAGCAATGAACCGACCATCCATGGCAAAGGCCAGCCGACCTTGCTGGCGGCAAAACCGCCAGCAAGGCCGACCAGCCCGGTCGCGACGAACAATGGCAACGACCGATCAGGCATCGGCCAGGGCCCGGCGCTGCAGGCTGc                                                         >  1:809119/1‑150 (MQ=255)
                                                                                          gatgatCGCCAGCAATGAACCGACCATCCATGGCAAAGGCCAGCCGACCTTGCTGGCGGCAAAACCGCCAGCAAGGCCGACCAGCCCGGTCGCGACAAACAATGGCAACGACCGATCAGGCATCGGCCAGGGCCCGGCGCTGCAGGCTGc                                                         <  2:3257755/150‑1 (MQ=255)
                                                                                          gatgatCCCCAGCAATCACCCCACCACCCATGGCACCGCCCCGCCGACCTTGCTGGCGGAAAACCCGCCAGCAAGGCCGACCAGCCCGGTCGCGACGAACAATGGCAACGACCGATCAGGCATCGGCCAGGGCCCGGCGCTGCAGGCTGc                                                         <  1:3038045/150‑1 (MQ=255)
                                                                                              atCGCCAGCAATGAACCGACCATCCATGGCAACGGCCCGCCGACCTGGGGGGCGCCAAAACCGCCAGAATGGCCGACCAGCCCGGTCGCGACGAACAATGGCAACGACCGATCAGGCATCGGCCAGGGCCCGGCGCTGCAGGCTGCGttt                                                     <  1:3469438/150‑1 (MQ=255)
                                                                                              atCGCAAGCATTGACCCCACCATCCTTGCCAACGGCCCGCCGACCTGGCTGGCGCCAAACCCGCCAGCACGGCCGACCAGCCCGGTCGCGACGAACAATGGCAACGACCGATCAGGCATCGGCCAGGGCCCGGCGCTGCAGGCTGCGttt                                                     <  1:4220546/150‑1 (MQ=255)
                                                                                               tCGCCAGCAATGAACCGACCATCCATGGCAAAGGCCAGCCGACCTTGCTGGCGGCAAAAACGCCAGCAAGGCCGACCAGCCCGGTCGCGACGAACAATGGCCACCACCGATCACGCATCGGCCAGGGCCCGGCGCTGCAGGCTGCGTTTg                                                    >  1:2572315/1‑150 (MQ=255)
                                                                                                cGCCAGCAATCAACCGCCCACCCATGGCAACGCCCAGCCGCCCTGGCGGGCGGCAAACCCGCCAGCACGGCCGACCAGCCCGGTCGCGACGAACAATGGCAACGACCGATCAGGCATCGGCCAGGGCCCGGCGCTGCAGGCTGCGTTTgc                                                   <  2:228932/150‑1 (MQ=255)
                                                                                                  ccAGCAATGAACCGACCATCCATGGCAAAGGCCAGCCGACCTTGCTGGCGGCAAAACCGCCAGCAAGGCCGACCAGCCCGGTCGCGACGAACAATGGCAACGACCGATCAGGCATCGGCCAGGGCCCGGCGCTGCAGGCTGCGTTTgcgc                                                 <  2:809108/150‑1 (MQ=255)
                                                                                                   cAGCAATGAACCGACCATCCATGGCAAAGGCCAGCCGACCTTGCTTGCGGCAAAAACGCCAGCAAGGCCGACCAGCCCGGTCGCGACGAACAATGGCAACGACCGATCAGGCATCGGCCAGGGCCCGGCGCTGCAGGCTGCGTTTGCGcc                                                >  1:129530/1‑150 (MQ=255)
                                                                                                   cAGCAATGAACCGACCATCCATGGCAAAGGCCAGCCGACCTTGCTGGCGGCAAAACCGCCAGCAAGGCCGACCCGCCCGGTCGCGACGAACAATGGCAACGACCGATCAGGCATCGGCCAGGGCCCGGCGCTGCAGGCTGCGTGTGCGcc                                                >  2:814944/1‑150 (MQ=255)
                                                                                                        aTGAACCGACCATCCATGGCAAAGGCCAGCCGACCTTGCTGGCGGCAAAACCGCCAGCAAGGCCGACCAGCCCGGTCGCGACGAACAATGGCAACGACCGATCAGGCATCGGCCAGGGCCCGGCGCTGCAGGCTGCGTTTGCGCCAGATg                                           >  2:837821/1‑150 (MQ=255)
                                                                                                        aTGAACCGACCATCCATGGCAAAGGCCAGCCGACCTTGCTGGCGGCAAAACCGCCAGCAAGGCCGACCAGCCCGGTCGCGACGAACAATGGCAACGACCGATCAGGCATCGGCCAGGGCCCGGCGCTGCAGGCTGCGTTTGCGCCAGATg                                           >  2:4449312/1‑150 (MQ=255)
                                                                                                          gAACCGACCATCCTTGGCAAAGGCCAGCCGACCTTGCTGGCGGCAAAACCGCCTGCAAGGCCGACCAGCCCGGTCGCGACGAACAATGGCAACGACCGATCAGGCATCGGCCAGGGCCCGGCGCTGCAGGCTGCGTTTGCGCCAGATgcg                                         <  1:3926130/150‑1 (MQ=255)
                                                                                                          gAACCGACCATCCATGGCAAAGGCCAGCCGACCTTGCTGGCGGCAAAACCGCCAGCAAGGCCGACCAGCCCGGTCGCGACGAACAATGGCAACGACCGATCAGGCATCGGCCAGGGCCCGGCGCTGCAGGCTGCGTTTGCGCCAGATgcg                                         >  2:36669/1‑150 (MQ=255)
                                                                                                          gAACCGACCATCCATGGCAAAGGCCAGCCGACCTTGCTGGCGGCAAAACCGCCAGCAAGGCCGACCAGCCCCGTCGCGCCGAACAATGGCAACGACCGATCAGGCATCGGCCAGGGCCCGGCGCTGCAGGCTGCGGTTGCGCCCGATgcg                                         >  1:233386/1‑150 (MQ=255)
                                                                                                              cGACCATCCATGGCAAAGGCCAGCCGACCTTGCTGGCGGCAAAACCGCCAGCAAGGCCGACCAGCCCGGTCGCGACGAACAATGGCAACGACCGATCAGGCATCGGCCAGGGCCCGGCGCTGCAGGCTGCGTTTGCGCCAGATGCgcagc                                     >  1:3961182/1‑150 (MQ=255)
                                                                                                               gACCATCCATGGCAAAGGCCAGCCGACCTTGCTGGCGGCAAAACCGCCAGCAAGGCCGACCAGCCCGGTCGCGACGAACAATGGCAACGACCGATCAGGCATCGGCCAGGGCCCGGCGCTGCAGGCTGCGTTTGCGCCAGATGCgcagca                                    >  2:2600087/1‑150 (MQ=255)
                                                                                                                   atccatGGCAACGGCCAGCCGACCTGGCGGGCGGCAAAACCGCCAGCAAGGCCGACCAGCCCGGTCGCGACGAACAATGGCAACGACCGATCAGGCATCGGCCAGGGCCCGGCGCTGCAGGCTGCGTTTGCGCCAGATGCGCAGCAGCgg                                <  2:2780686/150‑1 (MQ=255)
                                                                                                                    tccatGGCAAAGGCCAGCCGACCTTGCTGGCGGCAAAACCGCCAGCAAGGCCGACCAGCCCGGGCGCGACGAACAATGGCAACGACCGATCAGGCATCGGCCAGGGCCCGGCGCTGCAGGCTGCGTTTGCGCCAGATGCGCAGCAGCGGc                               >  2:2166339/1‑150 (MQ=255)
                                                                                                                    cccatGGCAAAGGCCAGCCGACCTGCCTGGCGGCAAACCCGCCAGCAAGGCCGCCCAGCCCGGTCGCGACGAACAATGGCAACGACCGATCAGGCATCGGCCAGGGCCCGGCGCTGCAGGCTGCGTTTGCGCCAGATGCGCAGCAGCGGc                               <  1:2205663/149‑1 (MQ=255)
                                                                                                                     ccttGGCAACGGCCAGCCGACGGGGCGGGCGGAAACACCCCCAGCACGCCCGACCAGCCCGGTCGCGACGAACAATGGCAACGACCGATCAGGCATCGGCCAGGGCCCGGCGCTGCAGGCTGCGTTTGCGCCAGATGCGCAGCAGCGGCa                              <  1:1859094/147‑1 (MQ=255)
                                                                                                                     ccatGGCAAAGGCCAGCCGACCTTGCTGGCGGCAAAACCGCCAGCAAGGCCGACCAGCCCGGTCGCGACGAACAATGGCAACGACCGATCAGGCATCGGCCAGGGCCCGGCGCTGCAGGCTGCGTTTGCGCCAGATGCGCAGCAGCGGCa                              <  2:4935508/150‑1 (MQ=255)
                                                                                                                      catGGCAACGGCCAGCCGACCTTGCTGGCGGCAAAACCGCCAGCAAGGCCGACCAGCCCGGTCGCGACGAACAATGGCAACGACCGATCAGGCATCGGCCAGGGCCCGGCGCTGCAGGCTGCGTTTGCGCCAGATGCGCAGCAGCGGCAg                             <  2:2699524/150‑1 (MQ=255)
                                                                                                                          gCAAAGGCCAGCCGACCTTGCTGGCGGCCAAACCCCCAGCAAGGCCGACCAGCCCCGTCGCGACGAACAATGGCAACGACCGATCAGGCATCGGCCAGGGCCCGGCGCTGCAGGCTGCGTTTTCGCCAGATGCGCAGCAGCGGCAGGGCg                         >  1:4794203/1‑150 (MQ=255)
                                                                                                                             acgccccgCCGACCTGGCTGGCGGAAAAACCGCCAGCACGGCCGACCAGCCCGGTCGCGACGAACAATGGCAACGACCGATCAGGCATCGGCCAGGGCCCGGCGCTGCAGGCTGCGTTTGCGCCAGATGCGCAGCAGCGGCAGGGCGAGc                      <  1:1917994/143‑1 (MQ=255)
                                                                                                                              aGGCCAGCCGACCTTGCTGGCGGCAAAACCGCCAGCAAGGCCGACCAGCCCGGTCGCGACGAACAATGGCAACGACCGATCAGGCATCGGCCAGGGCCCGGCGCTGCAGGCTGCGTTTGCGCCAGATGCGCAGCAGCGGCAGGGCGAGCa                     >  2:5428332/1‑150 (MQ=255)
                                                                                                                               ggCCACCCGACCTTGCTGGCGGCAAAACCGCCAGCACGGCCGACCAGCCCGGTCGCGACGAACAATGGCAACGACCGATCAGGCATCGGCCAGGGCCCGGCGCTGCAGGCTGCGTTTGCGCCAGATGCGCAGCAGCGGCAGGGCGAGCAt                    <  2:1432682/150‑1 (MQ=255)
                                                                                                                                 ccAGACGACCTTGCTGGCGGCAAAACCGCCAGCAAGGCCGACCAGCCCGGTCGCGACGAACAATGGCAACGACCGATCAGGCATCGGCCAGGGCCCGGCGCTGCAGGCTGAGTTTGCGCCAGATGCGCAGCAGCGGCAGGGCGAGCATTa                  >  2:5363233/1‑150 (MQ=255)
                                                                                                                                    gCCGACCTTGCTGGCGGCAAAACCGCCAGCAAGGCCGACCAGCCCGGTCGCGACGAACAATGGCAACGACCGATCAGGCATCGGCCAGGGCCCGGCGCTGCAGGCTGCGt                                                       <  1:3568629/110‑1 (MQ=255)
                                                                                                                                    gCCGACCTTGCTGGCGGCAAAACCGCCAGCAAGGCCGACCAGCCCGGTCGCGACGAACAATGGCAACGACCGATCAGGCATCGGCCAGGGCCCGGCGCTGCAGGCTGCGt                                                       >  2:3568629/1‑110 (MQ=255)
                                                                                                                                    ccccaCCTTGCTGGGGGAAAACCCGCAAGCACGCCCGACCAGCCCGGTCGCGACGAACAATGGCAACGACCGATCAGGCATCGGCCAGGGCCCGGCGCTGCAGGCTGCGTTTGCGCCAGATGCGCAGCAGCGGCATGGCGAGCATTACCa               <  1:2792761/146‑1 (MQ=255)
                                                                                                                                      cggccTTGCTGGCGGTTTTGCCGCCAGCAAGGTCGACCAGCCCGGTCGCGACGAACAATGGCAACGACCGATCAGGCATCGGCCAGGGCCCGGCGCTGCAGGCTGCGTTTGCGCCAGATGCGCAGCAGCGGCAGGGCGAGCATTACCACt             >  2:4255831/4‑150 (MQ=255)
                                                                                                                                        gccTTGCTGGCGGTTTTGCCGCCAGCAAGGCCGACCAGCCCGGTCGCGACGAACAATGGCAACGACCGATCAGGCATCGGCCAGGGCCCGGCGCTGCAGGCTGCGTTTGCGCCAGATGCGCAGCAGCGGCCGGGCGAGCATTACCACTAc           >  2:4716743/2‑150 (MQ=255)
                                                                                                                                        gccTTGCTGGCGGTTTTGCCGCCAGCAAGGCCCACCAGCCCGGTCGCGACGAAAAATGGCAACGACCGATCAGGCAACGGCCAGGGCCCGGCGCTGCAGGCTGCGTTTGCGCCAGATGCGCAGCAGCGGCAGGGCGAGCATTACCACAAc           >  2:2275068/2‑150 (MQ=255)
                                                                                                                                        aCCTTGCTGGCGGCAAAACCGCCAGCAAGGCCGACCAGCCCGGTCGCGACGAACAATGGCAACGACCGATCAGGCATCGGCCAGGGCCCGGCGCTGCAGGCTGCGTTTGCGCCAGATGCGCAGCAGCGGCAGGGCGAGCATTACCACTAc           <  2:5049428/150‑1 (MQ=255)
                                                                                                                                        aCCTTGCTGGCGGCAAAACCGCCAGCAAGGCCGACCAGCCCGGTCGCGACGAACAATGGCAACGACCGATCAGGCATCGGCCAGGGCCCGGCGCTGCAGGCTGCGTTTGCGCCAGATGCGCAGCAGCGGCAGGGCGAGCATTACCACTAc           <  1:2033799/150‑1 (MQ=255)
                                                                                                                                          cTTGCTGGCGGTTTTGCCGCCAGCAAGGCCGACCAGCCCGGTCGCGACGAACAATGGCAACGACCGATCAGGCATCGGCCAGGGCCCGGCGCTGCAGGCTGCGtt                                                      >  1:2191145/1‑105 (MQ=38)
                                                                                                                                          cTTGCTGGCGGCAAAACCGCCAGCAAGGCCGACCAGCCCGGTCGCGACGAACAATGGCAACGACCGATCAGGCATCGGCCAGGGCCCGGCGCTGCAGGCTGCGTTTGCGCCAGATGCGCAGCAGCGGCAGGGCGAGCATTACCACTACCa         >  1:2067299/1‑150 (MQ=255)
                                                                                                                                               tGGCGGCAAAACCGCCAGCAAGGCCGACCAGCCCGGTCGCGACGAACAATGGCAACGACCGATCAGGCATCGGCCATGGCCCGGCGCTGCAGGCTGCGTTTGCGCCAGATGCGCAGCAGCGGCAGGGCGAGCAGTACCACTACCCGCGcc    >  2:4155994/1‑150 (MQ=255)
                                                                                                                                               tGGCGGCAAAACCGCCAGCAAGGCCGACCAGCCCGGTCGCGACGAACAATGGCAACGACCGATCAGGCATCGGCCAGGGCCCGGCGCTGCAGGCTGCGTTTGCGCCAGATGCGCAGCAGCGGCAGGGCGAGCATTACCACTACCAGCGcc    >  1:1582710/1‑150 (MQ=255)
                                                                                                                                               tGGCGGCAAAACCGCCAGCAAGGCCGACCAGCCCGGTCGCGACGAACAATGGCAACGACCGATCAGGCATCGGCCAGGGCCCGGCGCTGCAGGCTGCGTTTGCGCCAGATGCGCAGCAGCGGCAGGGCGAGCATTACCACTACCAGCGcc    >  2:2417296/1‑150 (MQ=255)
                                                                                                                                               tGGCGGCAAAACCGCCAGCAAGGCCGACCAGCCCGGTCGCGACGAACAATGGCAACGACCGATCAGGCATCGGCCAGGGCCCGGCGCTGCAGGCTGCGTTTGCGCCAGAGGCGCAGCAGCGGCAGGGCGAGCATTACCACTACCAGCGcc    >  2:3967497/1‑150 (MQ=255)
                                                                                                                                                 gcggcAAAACCGCCAGCAAGGCCGACCAGCCCGGTCGCGACGAACAATGGCAACGACCGATCAGGCATCGGCCAGGGCCCGGCGCTGCAGGCTGCGTTTGCGCCAGATGCGCAGCAGCGGCAGGGCGAGCATTACCACTACCAGCGCCCa  >  2:5331994/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                 
CAGGCCAATACCGATGCCGATGATCCACTGCCCGCACTTGCGGCCGTTGGGGATTTCCGAAAGCTGCCAACGCGTCAGGCAGCGCACCAGGATGATCGCCAGCAATGAACCGACCATCCATGGCAAAGGCCAGCCGACCTTGCTGGCGGCAAAACCGCCAGCAAGGCCGACCAGCCCGGTCGCGACGAACAATGGCAACGACCGATCAGGCATCGGCCAGGGCCCGGCGCTGCAGGCTGCGTTTGCGCCAGATGCGCAGCAGCGGCAGGGCGAGCATTACCACTACCAGCGCCCA  >  NC_002947/1615581‑1615875

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑