Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 1,615,731 A→T 6.8% F21L (TTT→TTA) ‡ PP_1415 ← membrane protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029471,615,7310AT 6.8% 419.4 / 14.7 143F21L (TTT→TTA) ‡PP_1415membrane protein
Reads supporting (aligned to +/- strand):  ref base A (81/48);  new base T (4/6);  total (89/54)
Fisher's exact test for biased strand distribution p-value = 1.86e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.44e-01

Only 100 of 146 total aligned reads displayed.

CAATACCGATGCCGATGATCCACTGCCCGCACTTGCGGCCGTTGGGGATTTCCGAAAGCTGCCAACGCGTCAGGCAGCGCACCAGGATGATCGCCAGCAATGAACCGACCATCCATGGCAAAGGCCAGCCGACCTTGCTGGCGGCAAAACCGCCAGCAAGGCCGACCAGCCCGGTCGCGACGAACAATGGCAACGACCGATCAGGCATCGGCCAGGGCCCGGCGCTGCAGGCTGCGTTTGCGCCAGATGCGCAGCAGCGGCAGGGCGAGCATTACCACTACCAGCGCCCAGATGC  >  NC_002947/1615586‑1615880
                                                                                                                                                 |                                                                                                                                                     
    aCCGATGCCGATGATCCACTGCCCGCACTTGCGGCCGTTGGGGATTTCCGAAAGCTGCCAACGCGTCAGGCAGCGCACCAGGATGATCGCCAGCAATGAACCGACCATCCATGGCAAAGGCCAGCCGACCTTGCTGGCGGCAAAACCGcc                                                                                                                                               <  1:4871624/150‑1 (MQ=255)
      cgatgccgatgATCCACTGCCCGCACTTGCGGCCGTTGGGGATTTCCGAAAGCTGCCAACGCGTCAGGCAGCGCACCAGGATGATCGCCAGCAATGAACCGACCATCCATGGCAAAGGCCAGCCGACCTTGCTGGCGGCAAAACCGCCAg                                                                                                                                             >  1:3144826/1‑150 (MQ=255)
         tgccgatgATCCACTGCCCGCACTTGCGGCCGTTGGGGATTTCCGAAAGCTGCCAACGCGTCAGGCAGCGCACCAGGATGATCGCCAGCAATGAACCGACCATCCATGGCAAAGGCCAGCCGACCTTGCTGGCGGCAAAACCGCCAGCaa                                                                                                                                          <  2:14847/150‑1 (MQ=255)
         tgccgatgATCCACTGCCCGCACTTGCGGCCGTTGGGGATTTCCGAAAGCTGCCAACGCGTCAGGCAGCGCACCAGGATGATCGCCAGCAATGAACCGACCATCCATGGCAAAGGCCAGCCGACCTTGCTGGCGGCAAAACCGCCAGCaa                                                                                                                                          <  2:1254647/150‑1 (MQ=255)
          gccgatgATCCACTGCCCGCACTTGCGGCCGTTGGGGATTTCCGAAAGCTGCCAACGCGTCAGGCAGCGCACCAGGATGATCGCCAGCAATGAACCGACCATCCATGGCAAAGGCCAGCCGACCTTGCTGGCGGCAAAACCGCCAGCAAg                                                                                                                                         <  2:4405030/150‑1 (MQ=255)
            cgatgATCCACTGCCCGCACTTGCGGCCGTTGGGGATTTCCGAAAGCTGCCAACGCGTCAGGCAGCGCACCAGGATGATCGCCAGCAATGAACCGACCATCCATGGCAAAGGCCAGCCGACCTTGCTGGCGGCAAAACCGCCAGCAAGGc                                                                                                                                       <  2:109588/150‑1 (MQ=255)
             gatgatCCACTGCCCGCACTTGCGGCCGTTGGGGATTTCCGAAAGCTGCCAACGCGTCAGGCCGCGCACCAGGATGATCGCCAGCAATGAACCGACCATCCATGGCAAAGGCCAGCCGACCTTGCTGGCGGCCAAACCGCCAGCAAGGcc                                                                                                                                      >  1:3660809/1‑150 (MQ=255)
             gatgatCCACTGCCCGCACTTGCGGCCGTTGGGGATTTCCGAAAGCTGCCAACGCGTCAGGCAGCGCACCAGGATGATCGCCAGCAATGAACCGACCATCCATGGCAAAGGCCAGCCGACCTTGCTGGCGGCAAAACCGCCAGCAAGGcc                                                                                                                                      >  1:3915073/1‑150 (MQ=255)
             gatgatCCACTGCCCGCACTTGCGGCCGTTGGGGATTTCCGAAAGCTGCCAACGCGTCAGGCAGCGCACCAGGATGATCGCCAGCAATGAACCGACCATCCATGGCAAAGGCCAGCCGACCTTGCTGGCGGCAAAACCGCCAGCAAGGcc                                                                                                                                      >  1:3660709/1‑150 (MQ=255)
             gatgatCCACTGCCCGCACTTGCGGCCGTTGGGGATTTCCGAAAGCTGCCAACGCGTCAGGCAGCGCACCAGGATGATCGCCAGCAATGAACCGACCATCCATGGCAAAGGCCAGCCGACCTTGCTGGCGGCAAAAACGCCAGCAAGGcc                                                                                                                                      >  1:1062369/1‑150 (MQ=255)
             gatgatCCACTGCCCGCACTCGCGGCCGTTGGGGATTTCCGAAAGCTGCCAACGCGTCAGGCAGCGCACCAGGATGATCGCCAGCAATGAACCGACCATCCATGGCAAAGGCCAGCCGACCTTGCTGGCGGTTTTGCCGCCAGCAAggtc                                                                                                                                      <  2:2983229/150‑3 (MQ=255)
             gatgatCCACTGCCCGCACTCGCGGCCGTTGGGGATTTCCGAAAGCTGCCAACGCGTCAGGCAGCGCACCACGATGATCGCCAGCAATGAACCGACCATCCATGGCAAAGGCCAGCCGACCTTCCTGGCGGTTTTGCCGCCAGCAAggtc                                                                                                                                      <  2:3466125/150‑3 (MQ=255)
                   ccACTGCCCGCACTTGCGGCCGTTGGGGATTTCCGAAAGCTGCCAACGCGTCAGGCAGCGCACCAGGATGATCGCCAGCAATGAACCGACCATCCATGGCAAAGGCCAGCCGACCTTGCTGGCGGCAAAACCGCCCGCAAGGCCGACCAg                                                                                                                                >  2:4702964/1‑150 (MQ=255)
                   ccACTGCCCGCACTTGCGGCCGTTGGGGATTTCCGAAAGCTGCCAACGCGTCAGGCAGCGCACCAGGATGATCGCCAGCAATGAACCGACCATCCATGGCAAAGGCCAGCCGACCTTGCTGGCGGCAAAACCGCCAGCAAGGCCCACCAg                                                                                                                                >  1:635310/1‑150 (MQ=255)
                    cACTGCCCGCACTTGCGGCCGTTGGGGATTTCCGAAAGCTGCCAACGCGTCAGGCAGCGCACCAGGATGATCGCCAGCAATGAACCGACCATCCATGGCAAAGGCCAGCCGACCTTGCTGGCGGCAAAACCGCCAGCAAGGCCGACCAGc                                                                                                                               >  2:3608613/1‑150 (MQ=255)
                     aCTGCCCGCACTTGCGGCCGTTGGGGATTTCCGAAAGCTGCCAACGCGTCAGGCAGCGCACCAGGATGATCGCCAGCAATGAACCGACCATCCATGGCAAAGGCCAGCCGACCTTGCTGGCGGCAAAACCGc                                                                                                                                                <  2:5102122/132‑1 (MQ=255)
                     aCTGCCCGCACTTGCGGCCGTTGGGGATTTCCGAAAGCTGCCAACGCGTCAGGCAGCGCACCAGGATGATCGCCAGCAATGAACCGACCATCCATGGCAAAGGCCAGCCGACCTTGCTGGCGGCAAAACCGc                                                                                                                                                >  1:5102122/1‑132 (MQ=255)
                     aCTGCCCGCACTTGCGGCCGTTGGGGATTTCCGAAAGCTGCCAACGCGTCAGGCAGCGCACCAGGATGATCGCCAGCAATGAACCGACCATCCATGGCAAAGGCCAGCCGACCTTGCTGGCGGCAAAACCGCCAGCAAGGCCGACCAGcc                                                                                                                              >  2:5172368/1‑150 (MQ=255)
                         cccGCACTTGCGGCCGTTGGGGATTTCCGAAAGCTGCCAACGCGTCAGGCAGCGCACCAGGATGATCGCCAGCAATGAACCGACCATCCATGGCAAAGGCCAGCCGACCTTGCTGGCGGCAAAACCGCCAGCAAGGCCGACCAGCCCGGt                                                                                                                          >  2:4346616/1‑150 (MQ=255)
                           cGCACTTGCGGCCGTTGGGGATTTCCGAAAGCTGCCAACGCGTCAGGCAGCGCACCAGGATGATCGCCAGCAATGAACCGACCATCCATGGCAAAGGCCAGCCGACCTTGCTGGCGGCAAAACCGCCAGCAACGCCGACCCGCCCGGTcg                                                                                                                        >  1:5174209/1‑150 (MQ=255)
                            gCACTTGCGGCCGTTGGGGATTTCCGAAAGCTGCCAACGCGTCAGGCAGCGCACCAGGATGATCGCCAGCAATGAACCGACCATCCATGGCAAAGGCCAGCCGACCTTGCTGGCGGCAAAACCCCCAGCAAGGCCGACCAGCCCGGTcgc                                                                                                                       >  1:2327572/1‑150 (MQ=255)
                             cACTTGCGGCCGTTGGGGATTTCCGAAAGCTGCCAACGCGTCAGGCAGCGCACCAGGATGATCGCCAGCAATGAACCGACCATCCATGGCAAAGGCCAGCCGACCATGCAGGCGGCAAAACCCCCACCAAAGCCGACCACCCCCGgcgcc                                                                                                                      >  1:952556/1‑149 (MQ=255)
                              aCTTGCGGCCGTTGGGGATTTCCGAAAGCTGCCAACGCGTCAGGCAGCGCACCAGGATGATCGCCAGCAATGAACCGACCATCCATGGCAAAGGCCAGCCGACCTTGCTGGCGGCAAAACCGCCAGCAAGGCCGACCAGCCCGGTCGcga                                                                                                                     <  2:5346251/150‑1 (MQ=255)
                              aCTTGCGGCCGTTGGGGATTTCCGAAAGCTGCCAACGCGTCAGGCAGCGCACCAGGATGATCGCCAGCAATGAACCGACCATCCATGGCAAAGGCCAGCCGACCTTGCTGGCGGCAAAACCGCCAGCAAGGCCGACCAGCCCGGTCGcga                                                                                                                     >  2:2735614/1‑150 (MQ=255)
                                ttGCGGCCGTTGGGGATTTCCGAAAGCTGCCAACGCGTCAGGCAGCGCACCAGGATGATCGCCAGCAATGAACCGACCATCCATGGCAAAGGCCAGCCGACCATGCATGCGGCAAAAACCCCAGCAAAGCCCACCACCCCCGTCGcgacc                                                                                                                   >  1:2436033/1‑149 (MQ=255)
                                     gCCGTTGGGGATTTCCGAAAGCTGCCAACGCGTCAGGCAGCGCACCAGGATGATCGCCAGCAATGAACCGACCATCCATGGCAAAGGCCAGCCGACCGTGCTGGCGGCCAAACCCCCCGCAAGGCCGACCAGCCCGGGCGCGACGAACaa                                                                                                              >  1:912480/1‑150 (MQ=255)
                                      cggTTGGGTATTTCCGAACGCTGCAAACGCGACTGGCAGCGCACCAGGATGACCGCCAGAAATGACCCCACCATCATTGGCAACGGCCAGCCGACTTTGGTGGGGGAAAACCCGCCAGCAAGGCCGACCAGCCCGGTCGCGACGAACAAt                                                                                                             <  1:4417656/148‑1 (MQ=255)
                                         ttGGGGATTTCCGAAAGCTGCCAACGCGTCAGGCAGCGCACCAGGATGATCGCCAGCAATGAACCGACCATCCATGGCAAAGGCCAGCCGACCTTGCTGGCGGCAAAACCGCCAGCAAGGCCGACCAGCCCGGTCGCGACGAACAATGGc                                                                                                          >  2:3179638/1‑150 (MQ=255)
                                             ggATTTCCGAAAGCTGCCAACGCGTCAGGCAGCGCACCAGGATGATCGCCAGCAATGAACCGACCATCCATGGCAAAGGCCAGCCGACCTTGCTGGCGGCAAAACCGCCAGCAAGGCCGACCAGCCCGGTCGCGACGAACAATGGCAACg                                                                                                      >  2:2121192/1‑150 (MQ=255)
                                             ggATTTCCGAAAGCTGCCAACGCGTCAGGCAGCGCACCAGGATGATCGCCAGCAATGAACCGACCATCCATGGCAAAGGCCAGCCGACCTTGCTGGCGGCAAAACCGCCAGCAAGGCCGACCAGCCCGGTCGCGACGAACAATGGCAACg                                                                                                      >  1:4233092/1‑150 (MQ=255)
                                             ggATTTCCGAAAGCTGCCAACGCGTCAGGCAGCGCACCAGGATGATCGCCAGCAATGAACCGACCATCCATGGCAAAGGCCAGCCGACCTTGCTGGCGGCAAAACCGCCAGCAAGGCCGACCAGCCCGGTCGCGACGAAACATGGCAACg                                                                                                      >  1:4233086/1‑150 (MQ=255)
                                              gATTTCCGAACGCTGCCAACGCGTCAGGCAGCGCACCAGGATGATCGCCAGCAATGAACCGACCATCCATGGCAACGGCCAGCCGACGTTGCTGGCGGCAAACCCGCCAGAAAGGCCGACCAGCCCGGTCGCGACGAACAATGGCAACGa                                                                                                     <  2:2077654/150‑1 (MQ=255)
                                                 ttCCGAAAGCTGCCAACGCGTCAGGCAGCGCACCAGGATGATCGCCAGCAATGAACCGACCATCCATGGCAAAGGCCAGCCGACCTTGCTGGCGGCAAAACCGCCAGCAAGGCCGACCAGCCCGGTCGCGACGAACAATGGCAACGACCg                                                                                                  >  2:3386581/1‑150 (MQ=255)
                                                 ttCCGAAAGCTGCCAACGCGTCAGGCAGCGCACCAGGATGATCGCCAGCAATGAACCGACCATCCATGGCAAAGGCCAGCCGACCTTGCTGGCGGCAAAACCGCCAGCAAGGCCGACCAGCCCGGTCGCGACGAACAATGGCAACGACCg                                                                                                  >  1:1152207/1‑150 (MQ=255)
                                                    cGAAAGCTGCCAACGCGTCAGGCAGCGCACCAGGATGATCGCCAGCAATGAACCGACCATCCATGGCAAAGGCCAGCCGACCTTGCTGGCGGCAAAACCGCCAGCAAGGCCGACCAGCCCGGTCGCGACGAACAATGGCAACGACCGATc                                                                                               >  1:1201161/1‑150 (MQ=255)
                                                    cGAAAGCTGCCAACGCGTCAGGCAGCGCACCAGGATGATCGCCAGCAATGAACCGACCATCCATGGCAAAGGCCAGCCGACCTTGCTGGCGGCAAAACCGCCAGCAAGGCCGACCAGCCCGGTCGCGACGAACAATGGCAACGACCGATc                                                                                               >  2:3545165/1‑150 (MQ=255)
                                                     gAAAGCTGCCAACGCGTCAGGCAGCGCACCAGGATGATCGCCAGCAATGAACCGACCATCCATGGCAAAGGCCAGCCGACCTTGCTGGCGGCAAAACCGCCAGCAAGGCCGACCAGCCCGGTCGCGACGAACAATGGCAACGACCGATCa                                                                                              >  1:4507094/1‑150 (MQ=255)
                                                      aaaGCTGCCAACGCGTCAGGCAGCGCACCAGGATGATCGCCAGCAATGAACCGACCATCCATGGCAAAGGCCAGCCGACCTTGCTGGCGGCAAAACCGCCAGCAAGGCCGACCAGCCCGGTCGCGACGAACAATGGCAACGACCGATCAg                                                                                             >  2:4272178/1‑150 (MQ=255)
                                                        aGCTGCCAACGCGTCAGGCAGCGCACCAGGATGATCGCCAGCAATGAACCGACCATCCATGGCAAAGGCCAGCCGACCTTGCTGGCGGCAAAAACGCCAGCAAGGCCGACCACCACGGTCGCCACCAACAATGGCAACGACCGAACAGGc                                                                                           >  1:3294335/1‑150 (MQ=255)
                                                             ccAACGCGTCAGGCAGCGCACCAGGATGATCGCCAGCAATGAACCGACCATCCATGGCAAAGGCCAGCCGACCTTGCTGGCGGCAAAACCGCCAGCAAGGCCGACCAGCCCGGTCGCGACGAACAATGGCAACGACCGATCAGGCATCgg                                                                                      >  2:3216505/1‑150 (MQ=255)
                                                             ccAACGCGTCAGGCAGCGCACCAGGATGATCGCCAGCAATGAACCGACCATCCATGGCAAAGGCCAGCCGACCTTGCTGGCGGCAAAACCGCCAGCAAGGCCGACCAGCCCGGGCGCGACGAACAATGGCCACGACCGATCAGGCATCgg                                                                                      >  1:230485/1‑150 (MQ=255)
                                                              cAACGCGTCAGGCAGCGCACCAGGATGATCGCCAGCAATGAACCGACCATCCATGGCAAAGGCCAGCCGACCTTGCTGGCGGCAAAACCGCCAGCAAGGCCGACCAGCCCGGTCGCGACGAACAAAGGCAACGACCGATCAGGCATCGGc                                                                                     >  1:5233280/1‑150 (MQ=255)
                                                                aCGCGTCTGGCAGCGCACCAGGATGATCGCCAGCAATGAACCGACCATCCATGGCAACGGCCAGCCGACCTTGCTGGCGGCAAAACCGCCAGCAAGGCCGACCAGCCCGGTCGCGACGAACAATGGCAACGACCGATCAGGCATCGGCCa                                                                                   <  1:4963548/150‑1 (MQ=255)
                                                                  gcgTCAGGCAGCGCACCAGGATGATCGCCAGCAATGAACCGACCATCCATGGCAAAGGCCAGCCGACCTTGCTGGCGGCAAAACCGCCAGCAAGGCCGACCAGCCCGGTCGCGACGAACAATGGCAACGACCGATCAGGCATCGGCCAgg                                                                                 >  2:4566020/1‑150 (MQ=255)
                                                                       aGGCCGCGCACCAGGATGATCGCCAGCAATGAACCGACCCTCCATGGCAAAGGCCAGCCGACCTTGCTGGCGGCAACACCGCCAGCAAGGCCGACCCGCCCGGGCGCGACGAACAATGGCCCCGACCGCTCAGGCCTCGGCCAGGGCCCg                                                                            >  2:3257734/1‑150 (MQ=255)
                                                                         gCAGCGCACCAGGATGATCGCCAGCAATGAACCGACCATCCATGGCAAAGGCCAGCCGACCTTGCTGGCGGCAAAACCGCCAGCAAGGCCGACCAGCCCGGTCGCGACGAACAATGGCAACGACCGATCAGGCATCGGCCAGGGCCCGgc                                                                          >  2:46401/1‑150 (MQ=255)
                                                                         gCAGCGCACCAGGATGATCGCCAGCAATGAACCGACCATCCATGGCAAAGGCCAGCCGACCTTGCTGGCGGCAAAACCGCCAGCAAGGCCGACCAGCCCGGTCGCGACGAACAATGGCAACGACCGATCAGGCATCGGCCAGGGCCCGgc                                                                          >  2:4845529/1‑150 (MQ=255)
                                                                           aGCGCACCAGGATGATCGCCAGCAATGAACCGACCATCCATGGCAAAGGCCAGCCGACCTTGCTGGCGGCCAAAACGCCCGCAAGGCCGACCAGCCCGGTCGCGACGAACAATGGCAACGACCCATCAGGCATCGGCCAGGGCCCGgcgc                                                                        >  1:630732/1‑150 (MQ=255)
                                                                           aGCGCACCAGGATGATCGCCAGCAATGAACCGACCATCCATGGCAAAGGCCAGCCGACCTTGCTGGCGGCAAAACCGCCAGCAAgg                                                                                                                                        <  2:749990/86‑1 (MQ=255)
                                                                               cACCAGGATGATCGCCAGCAATGAACCGACCATCCATGGCAAAGGCCAGCCGACCTTGCTGGCGGCAAAACCGCCAGCAAGGCCGACCAGCCCGGTCGCGACGAACAATGGCAACGACCGATCAGGCATCGGCCAGGGCCCGGCGCTGCa                                                                    >  2:2305524/1‑150 (MQ=255)
                                                                                     gatgatCGCCAGCAATGAACCGACCATCCATGGCAAAGGCCAGCCGACCTTGCTGGCGGCAAAACCGCCAGCAAGGCCGACCAGCCCGGTCGCGACGAACAATGGCAACGACCGATCAGGCATCGGCCAGGGCCCGGCGCTGCAGGCTGc                                                              >  1:809119/1‑150 (MQ=255)
                                                                                     gatgatCGCCAGCAATGAACCGACCATCCATGGCAAAGGCCAGCCGACCTTGCTGGCGGCAAAACCGCCAGCAAGGCCGACCAGCCCGGTCGCGACGAACAATGGCAACGACCGATCAGGCATCGGCCAGGGCCCGGCGCTGCAGGCTGc                                                              >  1:809108/1‑150 (MQ=255)
                                                                                     gatgatCGCCAGCAATGAACCGACCATCCATGGCAAAGGCCAGCCGACCTTGCTGGCGGCAAAACCGCCAGCAAGGCCGACCAGCCCGGTCGCGACAAACAATGGCAACGACCGATCAGGCATCGGCCAGGGCCCGGCGCTGCAGGCTGc                                                              <  2:3257755/150‑1 (MQ=255)
                                                                                     gatgatCCCCAGCAATCACCCCACCACCCATGGCACCGCCCCGCCGACCTTGCTGGCGGAAAACCCGCCAGCAAGGCCGACCAGCCCGGTCGCGACGAACAATGGCAACGACCGATCAGGCATCGGCCAGGGCCCGGCGCTGCAGGCTGc                                                              <  1:3038045/150‑1 (MQ=255)
                                                                                          tCGCCAGCAATGAACCGACCATCCATGGCAAAGGCCAGCCGACCTTGCTGGCGGCAAAAACGCCAGCAAGGCCGACCAGCCCGGTCGCGACGAACAATGGCCACCACCGATCACGCATCGGCCAGGGCCCGGCGCTGCAGGCTGCGTTTg                                                         >  1:2572315/1‑150 (MQ=255)
                                                                                             ccAGCAATGAACCGACCATCCATGGCAAAGGCCAGCCGACCTTGCTGGCGGCAAAACCGCCAGCAAGGCCGACCAGCCCGGTCGCGACGAACAATGGCAACGACCGATCAGGCATCGGCCAGGGCCCGGCGCTGCAGGCTGCGTTTgcgc                                                      <  2:809108/150‑1 (MQ=255)
                                                                                              cAGCAATGAACCGACCATCCATGGCAAAGGCCAGCCGACCTTGCTTGCGGCAAAAACGCCAGCAAGGCCGACCAGCCCGGTCGCGACGAACAATGGCAACGACCGATCAGGCATCGGCCAGGGCCCGGCGCTGCAGGCTGCGTTTGCGcc                                                     >  1:129530/1‑150 (MQ=255)
                                                                                              cAGCAATGAACCGACCATCCATGGCAAAGGCCAGCCGACCTTGCTGGCGGCAAAACCGCCAGCAAGGCCGACCCGCCCGGTCGCGACGAACAATGGCAACGACCGATCAGGCATCGGCCAGGGCCCGGCGCTGCAGGCTGCGTGTGCGcc                                                     >  2:814944/1‑150 (MQ=255)
                                                                                                   aTGAACCGACCATCCATGGCAAAGGCCAGCCGACCTTGCTGGCGGCAAAACCGCCAGCAAGGCCGACCAGCCCGGTCGCGACGAACAATGGCAACGACCGATCAGGCATCGGCCAGGGCCCGGCGCTGCAGGCTGCGTTTGCGCCAGATg                                                >  2:4449312/1‑150 (MQ=255)
                                                                                                   aTGAACCGACCATCCATGGCAAAGGCCAGCCGACCTTGCTGGCGGCAAAACCGCCAGCAAGGCCGACCAGCCCGGTCGCGACGAACAATGGCAACGACCGATCAGGCATCGGCCAGGGCCCGGCGCTGCAGGCTGCGTTTGCGCCAGATg                                                >  2:837821/1‑150 (MQ=255)
                                                                                                     gAACCGACCATCCTTGGCAAAGGCCAGCCGACCTTGCTGGCGGCAAAACCGCCTGCAAGGCCGACCAGCCCGGTCGCGACGAACAATGGCAACGACCGATCAGGCATCGGCCAGGGCCCGGCGCTGCAGGCTGCGTTTGCGCCAGATgcg                                              <  1:3926130/150‑1 (MQ=255)
                                                                                                     gAACCGACCATCCATGGCAAAGGCCAGCCGACCTTGCTGGCGGCAAAACCGCCAGCAAGGCCGACCAGCCCGGTCGCGACGAACAATGGCAACGACCGATCAGGCATCGGCCAGGGCCCGGCGCTGCAGGCTGCGTTTGCGCCAGATgcg                                              >  2:36669/1‑150 (MQ=255)
                                                                                                     gAACCGACCATCCATGGCAAAGGCCAGCCGACCTTGCTGGCGGCAAAACCGCCAGCAAGGCCGACCAGCCCCGTCGCGCCGAACAATGGCAACGACCGATCAGGCATCGGCCAGGGCCCGGCGCTGCAGGCTGCGGTTGCGCCCGATgcg                                              >  1:233386/1‑150 (MQ=255)
                                                                                                         cGACCATCCATGGCAAAGGCCAGCCGACCTTGCTGGCGGCAAAACCGCCAGCAAGGCCGACCAGCCCGGTCGCGACGAACAATGGCAACGACCGATCAGGCATCGGCCAGGGCCCGGCGCTGCAGGCTGCGTTTGCGCCAGATGCgcagc                                          >  1:3961182/1‑150 (MQ=255)
                                                                                                          gACCATCCTGGGCAACGGCCAGCCGCCGTTGCTGGCGGAAAAACCGCCAGCTCGGCCGACCAGCCCGGTCGCGACGAACAATGGCAACGACCGATCAGGCATCGGCCAGGGCCCGGCGCTGCAGGCTGCGTTTGCGCCAGATGCgcagca                                         <  1:2142480/150‑1 (MQ=255)
                                                                                                          gACCATCCATGGCAAAGGCCAGCCGACCTTGCTGGCGGCAAAACCGCCAGCAAGGCCGACCAGCCCGGTCGCGACGAACAATGGCAACGACCGATCAGGCATCGGCCAGGGCCCGGCGCTGCAGGCTGCGTTTGCGCCAGATGCgcagca                                         >  2:2600087/1‑150 (MQ=255)
                                                                                                              acccatGGCAAAGGCCAGCCGACCTTGCGGGCGGCAAAACCGCCAGCAAGGCCGACCAGCCCGGTCGCGACGAACAATGGCAACGACCGATCAGGCATCGGCCAGGGCCCGGCGCTGCAGGCTGCGTTTGCGCCAGATGCGCAGCAGCgg                                     <  2:2780599/148‑1 (MQ=255)
                                                                                                               tccatGGCAAAGGCCAGCCGACCTTGCTGGCGGCAAAACCGCCAGCAAGGCCGACCAGCCCGGTCGCGACGAACAATGGCAACGACCGATCAGGCATCGGCCCGGGCCCGGCGCTGCAGGCTGCGTTTGCGCCAGATGCGCCGCAGCGGc                                    >  2:353143/1‑150 (MQ=255)
                                                                                                               tccatGGCAAAGGCCAGCCGACCTTGCTGGCGGCAAAACCGCCAGCAAGGCCGACCAGCCCGGTCGCGACGAACAATGGCAACGACCGATCAGGCATCGGCCAGGGCCCGGCGCTGCAGGCTGCGTTTGCGCCAGATGCGCAGCAGCGGc                                    >  2:2593323/1‑150 (MQ=255)
                                                                                                                ccttGGCAACGGCCAGCCGACGGGGCGGGCGGAAACACCCCCAGCACGCCCGACCAGCCCGGTCGCGACGAACAATGGCAACGACCGATCAGGCATCGGCCAGGGCCCGGCGCTGCAGGCTGCGTTTGCGCCAGATGCGCAGCAGCGGCa                                   <  1:1859094/147‑1 (MQ=255)
                                                                                                                ccatGGCAAAGGCCAGCCGACCTTGCTGGCGGCAAAACCGCCAGCAAGGCCGACCAGCCCGGTCGCGACGAACAATGGCAACGACCGATCAGGCATCGGCCAGGGCCCGGCGCTGCAGGCTGCGTTTGCGCCAGATGCGCAGCAGCGGCa                                   <  2:4935508/150‑1 (MQ=255)
                                                                                                                     gCAAAGGCCAGCCGACCTTGCTGGCGGCCAAACCCCCAGCAAGGCCGACCAGCCCCGTCGCGACGAACAATGGCAACGACCGATCAGGCATCGGCCAGGGCCCGGCGCTGCAGGCTGCGTTTTCGCCAGATGCGCAGCAGCGGCAGGGCg                              >  1:4794203/1‑150 (MQ=255)
                                                                                                                      cAAAGGCCAGCCGACCTGGCTGGCGGCAAAACCGCCAGCAAGGCCGACCAGCCCGGTCGCGACGAACAATGGCAACGACCGATCAGGCATCGGCCAGGGCCCGGCGCTGCAGGCTGCGTTTGCGCCAGATGCGCAGCAGCGGCAGGGCGa                             <  1:4348311/150‑1 (MQ=255)
                                                                                                                       aaaGGCCAGCCGACCTTGCTGGCGGCAAAAACGCCAGAAAGGCCGAACAGCCCGGTCGCGAAGAACAAATGCAAAGAACGATCAGGCATATGCCATGGCCCGGCGCAGCAGGCTGCGTTTTCGCCAGATTCGCAGCAGCGGCAGGGCGAg                            >  1:5276724/1‑150 (MQ=255)
                                                                                                                        acgccccgCCGACCTGGCTGGCGGAAAAACCGCCAGCACGGCCGACCAGCCCGGTCGCGACGAACAATGGCAACGACCGATCAGGCATCGGCCAGGGCCCGGCGCTGCAGGCTGCGTTTGCGCCAGATGCGCAGCAGCGGCAGGGCGAGc                           <  1:1917994/143‑1 (MQ=255)
                                                                                                                        aaGGCCAGCCGACCTTGCTGGCGGCAAAACCGCCAGCAAGGCCGACCAGCCCGGTCGCGACGAACAATGGCAACGACCGATCAGGCATCGGCCAGGGCCCGGCGCTGCAGGCTGCGTTTGCGCCAGATGCGCAGCAGCGGCAGGGCGAGc                           >  2:5264359/1‑150 (MQ=255)
                                                                                                                         aGGCCAGCCGACCTTGCTGGCGGCAAAACCGCCAGCAAGGCCGACCAGCCCGGTCGCGACGAACAATGGCAACGACCGATCAGGCATCGGCCAGGGCCCGGCGCTGCAGGCTGCGTTTGCGCCAGATGCGCAGCAGCGGCAGGGCGAGCa                          >  2:5428332/1‑150 (MQ=255)
                                                                                                                          ggCCACCCGACCTTGCTGGCGGCAAAACCGCCAGCACGGCCGACCAGCCCGGTCGCGACGAACAATGGCAACGACCGATCAGGCATCGGCCAGGGCCCGGCGCTGCAGGCTGCGTTTGCGCCAGATGCGCAGCAGCGGCAGGGCGAGCAt                         <  2:1432682/150‑1 (MQ=255)
                                                                                                                            ccAGACGACCTTGCTGGCGGCAAAACCGCCAGCAAGGCCGACCAGCCCGGTCGCGACGAACAATGGCAACGACCGATCAGGCATCGGCCAGGGCCCGGCGCTGCAGGCTGAGTTTGCGCCAGATGCGCAGCAGCGGCAGGGCGAGCATTa                       >  2:5363233/1‑150 (MQ=255)
                                                                                                                               gCCGACCTTGCTGGCGGCAAAACCGCCAGCAAGGCCGACCAGCCCGGTCGCGACGAACAATGGCAACGACCGATCAGGCATCGGCCAGGGCCCGGCGCTGCAGGCTGCGt                                                            >  2:3568629/1‑110 (MQ=255)
                                                                                                                               gCCGACCTTGCTGGCGGCAAAACCGCCAGCAAGGCCGACCAGCCCGGTCGCGACGAACAATGGCAACGACCGATCAGGCATCGGCCAGGGCCCGGCGCTGCAGGCTGCGt                                                            <  1:3568629/110‑1 (MQ=255)
                                                                                                                               gCCGACCTTGCTGGCGGCAAAACCGCCAGCAAGGCCGACCAGCCCGGTCGCGACGAACAATGGCAACGACCGATCAGGCATCGGCCAGGGCCCGGCGCTGCAGGCTGCGt                                                            <  1:3568557/110‑1 (MQ=255)
                                                                                                                               gCCGACCTTGCTGGCGGCAAAACCGCCAGCAAGGCCGACCAGCCCGGTCGCGACGAACAATGGCAACGACCGATCAGGCATCGGCCAGGGCCCGGCGCTGCAGGCTGCGt                                                            >  2:3568557/1‑110 (MQ=255)
                                                                                                                               gCCGACCTTGCTGGCGGCAAAACCGCCAGCAAGGCCGACCAGCCCGGTCGCGACGAACAATGGCAACGACCGATCAGGCATCGGCCAGGGCCCGGCGCTGCAGGCTGCGTTTGCGCCAGATGCGCAGCAGCGGCAGGGCGAGCATTACCa                    <  2:5118807/150‑1 (MQ=255)
                                                                                                                               ccccaCCTTGCTGGGGGAAAACCCGCAAGCACGCCCGACCAGCCCGGTCGCGACGAACAATGGCAACGACCGATCAGGCATCGGCCAGGGCCCGGCGCTGCAGGCTGCGTTTGCGCCAGATGCGCAGCAGCGGCATGGCGAGCATTACCa                    <  1:2792761/146‑1 (MQ=255)
                                                                                                                                   gccTTGCTGGCGGTTTTGCCGCCAGCAAGGCCGACCAGCCCGGTCGCGACGAACAATGGCAACGACCGATCAGGCATCGGCCAGGGCCCGGCGCTGCAGGCTGCGTTTGCGCCAGATGCGCAGCAGCGGCCGGGCGAGCATTACCACTAc                >  2:4716743/2‑150 (MQ=255)
                                                                                                                                   gccTTGCTGGCGGTTTTGCCGCCAGCAAGGCCCACCAGCCCGGTCGCGACGAAAAATGGCAACGACCGATCAGGCAACGGCCAGGGCCCGGCGCTGCAGGCTGCGTTTGCGCCAGATGCGCAGCAGCGGCAGGGCGAGCATTACCACAAc                >  2:2275068/2‑150 (MQ=255)
                                                                                                                                   aCCTTGCTGGCGGCAAAACCGCCAGCAAGGCCGACCAGCCCGGTCGCGACGAACAATGGCAACGACCGATCAGGCATCGGCCAGGGCCCGGCGCTGCAGGCTGCGTTTGCGCCAGATGCGCAGCAGCGGCAGGGCGAGCATTACCACTAc                <  2:5049428/150‑1 (MQ=255)
                                                                                                                                   aCCTTGCTGGCGGCAAAACCGCCAGCAAGGCCGACCAGCCCGGTCGCGACGAACAATGGCAACGACCGATCAGGCATCGGCCAGGGCCCGGCGCTGCAGGCTGCGTTTGCGCCAGATGCGCAGCAGCGGCAGGGCGAGCATTACCACTAc                <  1:2033799/150‑1 (MQ=255)
                                                                                                                                     cTTGCTGGCGGTTTTGCCGCCAGCAAGGCCGACCAGCCCGGTCGCGACGAACAATGGCAACGACCGATCAGGCATCGGCCAGGGCCCGGCGCTGCAGGCTGCGtt                                                           >  1:2191145/1‑105 (MQ=38)
                                                                                                                                     cTTGCTGGCGGTTTTGCCGCCAGCAAGGCCGACCAGCCCGGTCGCGACGAACAATGGCAACGACCGATCAGGCATCGGCCAGGGCCCGGCGCTGCAGGCTGCGtt                                                           <  2:2191145/105‑1 (MQ=38)
                                                                                                                                     cTTGCTGGCGGCAAAACCGCCAGCAAGGCCGACCAGCCCGGTCGCGACGAACAATGGCAACGACCGATCAGGCATCGGCCAGGGCCCGGCGCTGCAGGCTGCGTTTGCGCCAGATGCGCAGCAGCGGCAGGGCGAGCATTACCACTACCa              >  1:2067299/1‑150 (MQ=255)
                                                                                                                                          tGGCGGCAAAACCGCCAGCAAGGCCGACCAGCCCGGTCGCGACGAACAATGGCAACGACCGATCAGGCATCGGCCATGGCCCGGCGCTGCAGGCTGCGTTTGCGCCAGATGCGCAGCAGCGGCAGGGCGAGCAGTACCACTACCCGCGcc         >  2:4155994/1‑150 (MQ=255)
                                                                                                                                          tGGCGGCAAAACCGCCAGCAAGGCCGACCAGCCCGGTCGCGACGAACAATGGCAACGACCGATCAGGCATCGGCCAGGGCCCGGCGCTGCAGGCTGCGTTTGCGCCAGATGCGCAGCAGCGGCAGGGCGAGCATTACCACTACCAGCGcc         >  2:2417296/1‑150 (MQ=255)
                                                                                                                                          tGGCGGCAAAACCGCCAGCAAGGCCGACCAGCCCGGTCGCGACGAACAATGGCAACGACCGATCAGGCATCGGCCAGGGCCCGGCGCTGCAGGCTGCGTTTGCGCCAGATGCGCAGCAGCGGCAGGGCGAGCATTACCACTACCAGCGcc         >  2:3967487/1‑150 (MQ=255)
                                                                                                                                          tGGCGGCAAAACCGCCAGCAAGGCCGACCAGCCCGGTCGCGACGAACAATGGCAACGACCGATCAGGCATCGGCCAGGGCCCGGCGCTGCAGGCTGCGTTTGCGCCAGATGCGCAGCAGCGGCAGGGCGAGCATTACCACTACCAGCGcc         >  1:1582710/1‑150 (MQ=255)
                                                                                                                                          tGGCGGCAAAACCGCCAGCAAGGCCGACCAGCCCGGTCGCGACGAACAATGGCAACGACCGATCAGGCATCGGCCAGGGCCCGGCGCTGCAGGCTGCGTTTGCGCCAGAGGCGCAGCAGCGGCAGGGCGAGCATTACCACTACCAGCGcc         >  2:3967497/1‑150 (MQ=255)
                                                                                                                                            gcggcAAAACCGCCAGCAAGGCCGACCAGCCCGGTCGCGACGAACAATGGCAACGACCGATCAGGCATCGGCCAGGGCCCGGCGCTGCAGGCTGCGTTTGCGCCAGATGCGCAGCAGCGGCAGGGCGAGCATTACCACTACCAGCGCCCa       >  2:5331994/1‑150 (MQ=255)
                                                                                                                                              ggaaaaaCCGCCATCAACCCCGACCAGCCCGGTCGCGACGAACAATGGCAACGACCGATCAGGCATCGGCCAGGGCCCGGCGCTGCAGGCTGCGTTTGCGCCAGATGCGCAGCAGCGGCAGGGCGAGCATTACCACTACCAGCTCCCAGa     <  2:1762664/147‑1 (MQ=255)
                                                                                                                                               gaaaaaCCGACAGCAAGGCCGACCAGCCCGGTCGCGACGAACAATGGCAACGACCGATCAGGCATCGGCCAGGGCCCGGCGCTGCAGGCTGCGTTTGCGCCAGATGCGCAGCAGCGGCAGGGCGAGCATTACCACTACCAGCGCCCAGAt    <  1:4346378/148‑1 (MQ=255)
                                                                                                                                                 |                                                                                                                                                     
CAATACCGATGCCGATGATCCACTGCCCGCACTTGCGGCCGTTGGGGATTTCCGAAAGCTGCCAACGCGTCAGGCAGCGCACCAGGATGATCGCCAGCAATGAACCGACCATCCATGGCAAAGGCCAGCCGACCTTGCTGGCGGCAAAACCGCCAGCAAGGCCGACCAGCCCGGTCGCGACGAACAATGGCAACGACCGATCAGGCATCGGCCAGGGCCCGGCGCTGCAGGCTGCGTTTGCGCCAGATGCGCAGCAGCGGCAGGGCGAGCATTACCACTACCAGCGCCCAGATGC  >  NC_002947/1615586‑1615880

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑