BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	1,632,135	C→T	8.7%	P283S (CCT→TCT) ‡	lepA →	elongation factor 4

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	1,632,135	0	C	T	8.7%	468.8 / 25.4	161	P283S (CCT→TCT) ‡	lepA	elongation factor 4
Reads supporting (aligned to +/- strand): ref base C (71/76); new base T (9/5); total (80/81)
Fisher's exact test for biased strand distribution p-value = 2.78e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.99e-01

Only 100 of 163 total aligned reads displayed.

AGGTGCACTTGGTCGACAGCGTCGGTGTCTTCACCCCGAAGCACACCCAGACCGCTGATCTGAAAGCCGGCGAAGTAGGCTTCATCATCGCCAGCATCAAGGACATCCACGGTGCCCCGGTTGGTGACACCCTGACCCTGTCCTCGACCCCTGAGGTCGAGGTGTTGCCAGGCTTCAAGAAGATTCAGCCGCAGGTCTACGCCGGCCTGTTCCCGGTCAGCTCCGACGACTTCGAGGATTTCCGCGACGCTCTGCAGAAGCTGACCCTGAACGACTCCTCGCTGCAGTACATGCCGGAA  >  NC_002947/1631986‑1632284

aGGTGCACTTGGTCGACAGCGTCGGTGTCTTCACCCCGAAGCACACCCAGACCGCTGATCTGAAAGCCGGCGAAGTAGGCTTCATCATCGCCAGCATCAAGGACATCCACGGTGCCCCGGTTGGTGACACCCTGACCCTGTCCTCGAccc                                                                                                                                                       <  1:4071376/150‑1 (MQ=255)

          gggcGACAGCGTCGGGGTCGTCACCCCGAAGCACACCCAGACCGCTGATCTGAAAGCCGGCGAAGTAGGCTTCATCATCGCCAGCATCAAGGACATCCACGGTGCCCCGGTTGGTGACACCCTGACCCTGTCCTCGACCCCTGAGGTCGa                                                                                                                                             <  2:588266/147‑1 (MQ=255)

          ggTCGACAGCGTCGGTGTCTTCACCCCGAAGCACACCCAGACCGCTGATCTGAAAGCCGGCGAAGTAGGCTTCATCATCGCCAGCATCAAGGACATCCACGGTGCCCCGGTTGGTGACACCCTGACCCTGTCCTCGACCTCAGGGGTCGa                                                                                                                                             <  1:3033069/150‑1 (MQ=255)

          ggTCGACAGCGTCGGTGTCTTCACCCCGAAGCACACCCAGACCGCTGATCTGAAAGCCGGCGAAGTAGGCTTCATCATCGCCAGCATCAAGGACATCCACGGTGCCCCGGTTGGTGACACCCTGACCCTGTCCTCGACCCCTGAGGTCGa                                                                                                                                             <  2:588239/150‑1 (MQ=255)

              gACAGCGTCGGTGTCTTCACCCCGAAGCACACCCAGACCGCTGATCTGAAAGCCGGCGAAGTAGGCTTCATCATCGCCAGCATCAAGGACATCCACGGTGCCCCGGTTGGTGACACCCTGACCCTGTCCTCGACCCCTGAGGTCGAGgtg                                                                                                                                         >  2:4828195/1‑150 (MQ=255)

                cAGCGTCGGTGTCTTCACCCCGAAGCACACCCAGACCGCTGATCTGAAAGCCGGCGAAGTAGGCTTCATCATCGCCAGCATCAAGGACATCCACGGTGCCCCGGTTGGTGACACCCTGACCCTGTCCTCGACCCCTGAGGTCGAGgtcag                                                                                                                                       <  1:2652982/150‑4 (MQ=255)

                 aGCGGCGGTGTCTTCACCCCGAAGCACACCCAGACCGCTGATCTGAAAGCCGGCGAAGGAGGCTTCAGCATCGCCAGCAGCAAGGACATCCACGGTGCCGCGGTGGGTGCCACCCTGACCCGGTCCTCGACCCCGGCGGGCGCGGGGTTg                                                                                                                                      >  2:3672226/1‑150 (MQ=255)

                    gTCGGTGTCTTCACCCCGAAGCACACCCAGACCGCTGATCTGAAAGCCGGCGAAGTAGGCTTCATCATCGCCAGCATCAAGGACATCCACGGTGCCCCGGTTGGTGACACCCTGACCCTGTCCTCGACCCCTGAGGTCGAGGTGTTGCCa                                                                                                                                   <  2:2803562/150‑1 (MQ=255)

                    gTCGGTGTCTTCACCCCGAAGCACACCCAGACCGCTGATCTGAAAGCCGGCGAAGTAGGCTTCATCATCGCCAGCATCAAGGACATCCACGGTGCCCCGGTTGGTGACACCCTGACCCTGTCCTCGACCCCTGAGGTCGAGGTGTTGCCa                                                                                                                                   <  1:688109/150‑1 (MQ=255)

                      cGGTGTCTTCACCCCGAAGCACACCCAGACCGCTGATCTGAAAGCCGGCGAAGTAGGCTTCATCATCGCCAGCATCAAGGACATCCACGGTGCCCCGGTTGGTGACACCCTGACCCTGTCCTCGACCCCTGAGGTCGAGGTGTTGCCAgg                                                                                                                                 <  1:3410615/150‑1 (MQ=255)

                        gtgtCTTCACCCCGAAGCACACCCAGACCGCTGATCTGAAAGCCGGAGAAGTAGGCTTCATCATCGCCAGCATCAAGGACATCCACGGTGCCCCGGTTGGTGACACCCTGACCCTGTCCTCGACCCCTGAGGTCGAGGTGTTGCCAGGCt                                                                                                                               >  1:2814076/1‑150 (MQ=255)

                          gtCTTCACCCCGAAGCACACCCAGACCGCTGATCTGAAAGCCGGCGAAGTTGGCTTGATCATCGCCAGCATCAAGGACATCCACGGTGCCCCGGTTGGTGACACCCTGTCCCTGTCCTCGACCCCTGTGTTCGAGGTGTTGCCAGGCTTc                                                                                                                             <  2:147582/150‑1 (MQ=255)

                           tCTTCACCCCGAAGCACACCCAGACCGCTGATCTGAAAGCCGGCGAAGTAGGCTTCATCATCGCCAGCATCAAGGACATCCACGGTGCCCCGGTTGGTGACACCCTGACCCTGTCCTCGACCCCTGAGGTCGAGGTGTTGCCAGGCTTCa                                                                                                                            >  2:1369532/1‑150 (MQ=255)

                             ttCACCCCGAAGCACACCCAGACCGCTGATCTGAAAGCCGGCGAAGTAGGCTTCATCATCGCCAGCATCAAGGACATCCACGGTGCCCCGGTTGGTGACACCCTGACCCTGTCCTCGACCCCTGAGGTCGAGGTGTTGCCAGGCTTCaag                                                                                                                          <  1:4498698/150‑1 (MQ=255)

                             ttCACCCCGAAGCACACCCAGACCGCTGATCTGAAAGCCGGCGAAGTAGGCTTCATCATCGCCAGCATCAAGGACATCCACGGTGCCCCGGTTGGTGACACCCTGACCCTGTCCTCGACCCCTGAGGTCGAGGTGTTGCCAGGCTTCaag                                                                                                                          >  2:4199159/1‑150 (MQ=255)

                             ttCACCCCGAAGCACACCCAGACCGCTGATCTGAAAGCCGGCGAAGTAGGCTTCATCATCGCCAGCATCAAGGACATCCACGGTGCCCCGGTTGGTGACACCCTGACCCTGTCCTCGACCCCTGAGGTCGAGGTGTTGCCAGGCTTCaag                                                                                                                          >  1:864214/1‑150 (MQ=255)

                              tCACCCCGAAGCACACCCAGACCGCTGATCTGAAAGCCGGCGAAGTAGGCTTCATCATCGCCAGCATCAAGGACATCCACGGTGCCCCGGTTGGTGACACCCTGACCCTGTCCTCGACCCCTGAGGTCGAGGTGTTGCCAGGCTTCaaga                                                                                                                         <  2:3550615/150‑1 (MQ=255)

                                  cccGAAGCACACCCAGACCGCTGATCTGAAAGCCGGCGAAGTAGGCTTCATCATCGCCAGCATCAAGGACATCCACGGTGCCCCGGTTGGTGACACCCTGACCCTGTCCTCGACCCCTGAGGTCGAGGTGTTGCCAGGCTTCAAGAAGAt                                                                                                                     >  1:1659760/1‑150 (MQ=255)

                                   ccGAAGCACACCCAGACCGCTGATCTGAAAGCCGGCGAAGTAGGCTTCATCATCGCCAGCATCAAGGACATCCACGGTGCCCCGGTTGGTGACACCCTGACCCTGTCCTCGACCTCAGGGGTCGAggacagg                                                                                                                                      >  2:549596/1‑127 (MQ=255)

                                   ccGAAGCACACCCAGACCGCTGATCTGAAAGCCGGCGAAGTAGGCTTCATCATCGCCAGCATCAAGGACATCCACGGTGCCCCGGTTGGTGACACCCTGACCCTGTCCTCGACCTCAGGGGTCGAggacagg                                                                                                                                      <  1:549596/132‑6 (MQ=255)

                                   ccGAAGCACACCCAGACCGCTGATCTGAAAGCCGGCGAAGTAGGCTTCATCATCGCCAGCATCAAGGACATCCACGGTGCCCCGGTTGGTGACACCCTGACCCTGTCCTCGACCCCTGAGGTCGAGGTGTTGCCAGGCTTCAAGAAGAtt                                                                                                                    >  1:5440982/1‑150 (MQ=255)

                                    cGAAGCACACCCAGACCGCTGATCTGAAAGCCGGCGAAGTAGGCTTCATCATCGCCAGCATCAAGGACATCCACGGTGCCCCGGTTGGTGACACCCTGACCCTGTCCTCGACCCCTGAGGTCGAGGTGTTGcc                                                                                                                                    >  2:1275618/1‑133 (MQ=255)

                                    cGAAGCACACCCAGACCGCTGATCTGAAAGCCGGCGAAGTAGGCTTCATCATCGCCAGCATCAAGGACATCCACGGTGCCCCGGTTGGTGACACCCTGACCCTGTCCTCGACCCCTGAGGTCGAGGTGTTGcc                                                                                                                                    <  1:1275618/133‑1 (MQ=255)

                                     gAAGCACACCCAGACCGCTGATCTGAAAGCCGGCGAAGTAGGCTTCATCATCGCCAGCATCAAGGACATCCACGGTGCCCCGGTTGGTGACACCCTGACCCTGTCCTCGACCCCTGAGGTCGAGGTGTTGCCAGGCTTCAAGAAGATTCa                                                                                                                  <  1:1754956/150‑1 (MQ=255)

                                       aGCACACCCAGACCGCTGATCTGAAAGCCGGCGAAGTAGGCTTCATCATCGCCAGCATCAAGGACATCCACGGTGCCCCGGTTGGTGACACCCTGACCCTGTCCTCGACCCCTGAGGTCGAGGTGTTGCCAGGCTTCAAGAAGATTCAGc                                                                                                                <  1:880347/150‑1 (MQ=255)

                                           cacCCAGACCGCTGATCTGAAAGCCGGCGAAGTAGGCTTCATCCTCGCCAGCATCAAGGACATCCACGGTGCCCCGGTTGGTGACACCCTGACCCTGTCCTCGACCCCTGAGGTCGAGGTGTTGCCAGGCTTCAAGAAGATTCAGCCGcc                                                                                                            <  2:4598191/150‑2 (MQ=255)

                                           cacCCAGACCGCTGATCTGAAAGCCGGCGAAGTAGGCTTCATCATCGCCAGCATCAAGGACATCCACGGTGCCCCGGTTGGTGACACCCTGACCCTGTCCTCGACCCCTGAGGTCGAGGTGTTGCCAGGCTTCAAGAAGATTCAGCCGCa                                                                                                            <  1:163668/150‑1 (MQ=255)

                                            acCCAGACCGCTGATCTGAAAGCCGGCGAAGTAGGCTTCATCATCGCCAGCATCAAGGACATCCACGGTGCCCCGGTTGGTGACACCCTGACCCTGTCCTCGACCCCTGAGGTCGAGGTGTTGCCAGGCTTCAAGAAGATTCAGCCGCAg                                                                                                           >  2:4228790/1‑150 (MQ=255)

                                            acCCAGACCGCTGATCTGAAAGCCGGCGAAGTAGGCTTCATCATCGCCAGCATCAAGGACATCCACGGTGCCCCGGTTGGTGACACCCTGACCCTGTCCTCGACCCCTGAGGTCGAGGTGTTGCCAGGCTTCAAGAAGATTCAGCCGCAg                                                                                                           >  2:1880033/1‑150 (MQ=255)

                                              ccAGACCGCTGATCTGAAAGCCGGCGAAGTAGGCTTCATCATCGCCAGCATCAAGGACATCCACGGTGCCCCGGTTGGTGACACCCTGACCCTGTCCTCGACCCCTGAGGTCGAGGTGTTGCCAGGCTTCAAGAAGATTCAGCCGCAGGt                                                                                                         >  1:3577181/1‑150 (MQ=255)

                                              ccAGACCGCTGATCTGAAAGCCGGCGAAGTAGGCTTCATCATCGCCAGCATCAAGGACATCCACGGTGCCCCGGTTGGTGACACCCTGACCCTGTCCTCGACCCCTGAGGTCGAGGTGTTGCCAGGCTTCAAGAAGATTCAGCCGCAGGt                                                                                                         >  1:108064/1‑150 (MQ=255)

                                              ccAGACCGCTGATCTGAAAGCCGGCGAAGTAGGCTTCATCATCGCCAGCATCAAGGACATCCACGGTGCCCCGGTTGGTGACACCCTGACCCTGTCCTCGACCCCTGAGGTCGAGGTGTTGCCAGGCTTCAAGAAGATTCAGCCGCAGGt                                                                                                         >  2:786924/1‑150 (MQ=255)

                                                     gCTGATCTGAAAGCCGGCGAAGTAGGCTTCATCATCGCCAGCATCAAGGACATCCACGGTGCCCCGGTTGGTGACACCCTGACCCTGTCCTCGACCCCTGAGGTCGAGGTGTTGCCAGGCTTCAAGAAGATTCAGCCGCAGGTCTACGcc                                                                                                  >  2:514951/1‑150 (MQ=255)

                                                       tGATCTGAAAGCCGGCGAAGTAGGCTTCATCATCGCCAGCATCAAGGACATCCACGGTGCCCCGGTTGGTGACACCCTGACCCTGTCCTCGACCCCTGAGGTCGAGGTGTTGCCAGGc                                                                                                                                >  1:3270198/1‑118 (MQ=255)

                                                       tGATCTGAAAGCCGGCGAAGTAGGCTTCATCATCGCCAGCATCAAGGACATCCACGGTGCCCCGGTTGGTGACACCCTGACCCTGTCCTCGACCCCTGAGGTCGAGGTGTTGCCAGGc                                                                                                                                <  2:3270198/118‑1 (MQ=255)

                                                       tGATCTGAAAGCCGGCGAAGTAGGCTTCATCATCGCCAGCATCAAGGACATCCACGGTGCCCCGGTTGGTGACACCCTGACCCTGTCCTCGACCCCTGAGGTCGAGGTGTTGCCAGGCTTCAAGAAGATTCAGCCGCAGGTCTACGCCgg                                                                                                <  1:514951/150‑1 (MQ=255)

                                                        gATCTGAAAGCCGGCGAAGTAGGCTTCATCATCGCCAGCATCAAGGACATCCACGGTGCCCCGGTTGGTGACACCCTGACCCTGTCCTCGACCCCTGAGGTCGAGGTGTTGCCAGGCTTCAAGAAGATTCAGCCGCAGGTCTACGCCGGc                                                                                               <  1:2094801/150‑1 (MQ=255)

                                                        gATCTGAAAGCCGGCGAAGTAGGCTTCATCATCGCCAGCATCAAGGACATCCACGGTGCCCCGGTTGGTGACACCCTGACCCTGTCCTCGACCCCTGAGGTCGAGGTGTTGCCAGGCTTCAAGAAGATTCAGCCGCAGGTCTACGCCGGc                                                                                               <  1:2414295/150‑1 (MQ=255)

                                                        gATCTGAAAGCCGGCGAAGTAGGCTTCATCATCGCCAGCATCAAGGACATCCACGGTGCCCCGGTTGGTGACACCCTGACCCTGTCCTCGACCCCTGAGGTCGAGGTGTTGCCAGGCTTCAAGAAGATTCAGCCGCAGGTCTACGCCGGc                                                                                               <  1:4828195/150‑1 (MQ=255)

                                                            tGAAAGCCGGCGAAGTAGGCTTCATCATCGCCAGCATCAAGGACATCCACGGTGCCCCGGTTGGTGACACCCTGACCCTGTCCTCGACCCCTGAGGTCGAGGTGTTGCCAGGCTTCAAGAAGATTCAGCCGCAGGTCTACGCCGGCCTGt                                                                                           >  2:5069564/1‑150 (MQ=255)

                                                              aaaGCCGGCGAAGTAGGCTTCATCATCGCCAGCATCAAGGACATCCACGGTGCCCCGGTTGGTGACACCCTGACCCTGTCCTCGACCCCTGAGGTCGAGGTGTTGCCAGGCTTCAAGAAGATTCAGCCGCAGGTCTACGCCGGCCTGTTc                                                                                         <  1:2152740/150‑1 (MQ=255)

                                                                aGCCGGCGAAGTAGGCTTCATCATCGCCAGCATCAAGGACATCCACGGTGCCCCGGTTGGTGACACCCTGACCCTGTCCTCGACCCCTGAGGTCGAGGTGTTGCCAGGCTTCAAGAAGATTCAGCCGCAGGTCTACGCCGGCCTGTTccc                                                                                       >  2:4126028/1‑150 (MQ=255)

                                                                 gCCGGCGAAGTAGGCTTCATCATCGCCAGCATCAAGGACATCCACGGTGCCCCGGTTGGTGACACCCTGACCCTGTCCTCGACCCCTGAGGTCGAGGTGTTGCCAGGCTTCAAGAAGATTCAGCCGCAGGTCTACGCCGGCCTGTTCCCg                                                                                      <  1:3844133/150‑1 (MQ=255)

                                                                 gCCGGCGAAGTAGGCTTCATCATCGCCAGCATCAAGGACATCCACGGTGCCCCGGTTGGTGACACCCTGACCCTGTCCTCGACCCCTGAGGTCGAGGTGTTGCCAGGCTTCAAGAAGATTCAGCCGCAGGTCTACGCCGGCCTGTTCCCg                                                                                      <  1:472439/150‑1 (MQ=255)

                                                                 gCCGGCGAAGTAGGCTTCATCATCGCCAGCATCAAGGACATCCACGGTGCCCCGGTTGGTGACACCCTGACCCTGTCCTCGACCCCTGAGGTCGAGGTGTTGCCAGGCTTCAAGAAGATTCAGCCGCAGGTCTACGCCGGCCTGTTCCCg                                                                                      <  1:3844059/150‑1 (MQ=255)

                                                                   cGGCGAAGTAGGCTTCATCATCGCCAGCATCAAGGACATCCACGGTGCCCCGGTTGGTGACACCCTGACCCTGTCCTCGACCCCTGAGGTCGAGGTGTTGCCAGGCTTCAAGAAGATTCAGCCGCAGGTCTACGCCGGCCTGTTCCCGGt                                                                                    <  1:442597/150‑1 (MQ=255)

                                                                   cGGCGAAGTAGGCTTCATCATCGCCAGCATCAAGGACATCCACGGTGCCCCGGTTGGTGACACCCTGACCCTGTCCTCGACCCCTGAGGTCGAGGTGTTGCCAGGCTTCAAGAAGATTCAGCCGCAGGTCTACGCCGGCCTGTTCCCGGt                                                                                    >  2:1570623/1‑150 (MQ=255)

                                                                   cGGCGAAGTAGGCTTCATCATCGCCAGCATCAAGGACATCCACGGTGCCCCGGTTGGTGACACCCAGACCCGGTCCTCGACCCCTGAGGTCGAGGTGTGGCCAGGCTACAAGACGATTCAGCCGCAGGACTACGCCGGCCAGTTCCCGGt                                                                                    >  2:2831992/1‑150 (MQ=255)

                                                                     gCGAAGTAGGCTTCATCATCGCCAGCATCAAGGACATCCACGGTGCCCCGGTTGGTGACACCCTGACCCTGTCCTCGACCCCTGAGGTCGAGGTGTTGCCAGGCTTCAAGAAGATTCAGCCGCAGGTCTACGCCGGCCTGTTCCCGGTCa                                                                                  <  2:744911/150‑1 (MQ=255)

                                                                         aGTAGGCTTCATCATCGCCAGCATCAAGGACATCCACGGTGCCCCGGTTGGTGACACCCTGACCCTGTCCTCGACCCCTGAGGTCGAGGTGTTGCCAGGCTTCAAGAAGATTCAGCCGCAGGTCTACGCCGGCCTGTTCCCGGTCAGCTc                                                                              <  1:230123/150‑1 (MQ=255)

                                                                          gTAGGCTTCATCATCGCCAGCATCAAGGACATCCACGGTGCCCCGGTTGGTGACACCCTGACCCTGTCCTCGACCCCTGAGGTCGAGGTGTTGCCAGGCTTCAAGAAGATTCAGCCGCAGGTCTACGCCGGCCTGTTCCCGGTCAGCTcc                                                                             <  1:124315/150‑1 (MQ=255)

                                                                          gTAGGCTTCATCATCGCCAGCATCAAGGACATCCACGGTGCCCCGGTTGGTGACACCCTGACCCTGTCCTCGACCCCTGAGGTCGAGGTGTTGCCAGGCTTCAAGAAGATTCAGCCGCAGGTCTACGCCGGCCTGTTCCCGGTCAGCTcc                                                                             <  2:2078688/150‑1 (MQ=255)

                                                                            aGGCTTCATCATCGCCAGCATCAAGGACATCCACGGTGCCCCGGTTGGTGACACCCTGACCCTGTCCTCGACCCCTAAGGTCGAGGTGTTGCCAGGCTTCAAGAAGATTCAGCCGCAGGTCTACGCCGGCCTGTTCCCGGTCAGCTCcga                                                                           <  2:3079031/150‑1 (MQ=255)

                                                                                   atcatcGCCAGCATCAAGGACATCCACGGTGCCCCGGTTGGTGACACCCTGACCCTGTCCTCGACCCCTGAGGTCGAGGTGTTGCCAGGCTTCAAGAAGATTCAGCCGCAGGTCTACGCCGGCCTGTTCCCGGTCAGCTCCGACGACTTc                                                                    <  2:4948491/150‑1 (MQ=255)

                                                                                    tcatcGCCAGCATCAAGGACATCCACGGTGCCCCGGTTGGTGACACCCTGACCCTGTCCTCGACCCCTGAGGTCGAGGTGTTGCCAGGCTTCAAGAAGATTCAGCCGCAGGTCTACGCCGGCCTGTTCCCGGTCAGCTCCGACGACTTCg                                                                   >  1:2572996/1‑150 (MQ=255)

                                                                                     catcGCCAGCATCAAGGACATCCACGGTGCCCCGGTTGGTGACACCCTGACCCTGTCCTCGACCCCTGAGGTCGAGGTGTTGCCAGGCTTCAAGAAGATTCAGCCGCAGGTCTACGCCGGCCTGTTCCCGGTCAGCTCCGACGACTTCGa                                                                  <  1:2228446/150‑1 (MQ=255)

                                                                                     catcGCCAGCATCAAGGACATCCACGGTGCCCCGGTTGGTGACACCCTGACCCTGTCCTCGACCCCTGAGGTCGAGGTGTTGCCAGGCTTCAAGAAGATTCAGCCGCAGGTCTACGCCGGCCTGTTCCCGGTCAGCTCCGACGACTTCGa                                                                  <  1:2281681/150‑1 (MQ=255)

                                                                                     catcGCCAGCATCAAGGACATCCACGGTGCCCCGGTTGGTGACACCCTGACCCTGTCCTCGACCCCTGAGGTCGAGGTGTTGCCAGGCTTCAAGAAGATTCAGCCGCAGGTCTACGCCGGCCTGTTCCCGGTCAGCTCCGACGACTTCGa                                                                  <  2:5215702/150‑1 (MQ=255)

                                                                                              cATCAAGGACATCCTCGGTGCCCCGGTTGGTGACACCCTGACCCTGTCCTCGACCCCTGAGGTCGAGGTGTTGCCAGGCTTCAAGAAGATTCAGCCGCAGGTCTACGCCGGCCTGTTCCCGGTCAGCTCCGACGACTTCGAGGATTTCcg                                                         <  2:1006651/150‑1 (MQ=255)

                                                                                                   aGGACATCCACGGTGCCCCGGTTGGTGACACCCTGACCCTGTCCTCGACCCCTGAGGTCGAGGTGTTGCCAGGCTTCAAGAAGATTCAGCCGCAGGTCTACGCCGGCCTGTTCCCGGTCAGCTCCGACGACTTCGAGGATTTCCGCGACg                                                    >  1:3262425/1‑150 (MQ=255)

                                                                                                      aCATCCACGGTGCCCCGGTTGGTGACACCCTGACCCTGTCCTCGACCCCTGAGGTCGAGGTGTTGCCAGGCTTCAAGAAGATTCAGCCGCAGGTCTACGCCGGCCTGTTCCCGGTCAGCTCCGACGACTTCGAGGATTTCCGCGACGctc                                                 >  2:2033262/1‑150 (MQ=255)

                                                                                                          ccACGGTGCCCCGGTTGGTGACACCCTGACCCTGTCCTCGACCCCTGAGGTCGAGGTGTTGCCAGGCTTCAAGAAGATTCAGCCGCAGGTCTACGCCGGCCTGTTCCCGGTCAGCTCCGACGACTTCGAGGATTTCCGCGACGCTCTGCa                                             <  2:2814059/150‑1 (MQ=255)

                                                                                                              ggTGCCCCGGTTGGTGACACCCTGACCCTGTCCTCGACCCCTGAGGTCGAGGTGTTGCCAGGCTTCAAGAAGATTCAGCCGCAGGTCTACGCCGGCCTGTTCCCGGTCAGCTCCGACGACTTCGAGGATTTCCGCGACGCTCTGCAGAAg                                         >  2:2940762/1‑150 (MQ=255)

                                                                                                              ggTGCCCCGGTTGGTGACACCCTGACCCTGTCCTCGACCCCTGAGGTCGAGGTGTTGCCAGGCTTCAAGAAGATTCAGCCGCAGGTCTACGCCGGCCTGTTCCCGGTCAGCTCCGACGACTTCGAGGATTTCCGCGACGCTCTGCAGAAg                                         <  2:3552699/150‑1 (MQ=255)

                                                                                                               gTGCCCCGGTTGGTGACACCCTGACCCTGTCCTCGACCCCTGAGGTCGAGGTGTTGCCAGGCTTCAAGAAGATTCAGCCGCAGGTCTACGCCGGCCTGTTCCCGGTCAGCTCCGACGACTTCGAGGATTTCCGCGACGCTCTGCAGAAGc                                        <  2:4153634/150‑1 (MQ=255)

                                                                                                                  ccccGGTTGGTGACACCCTGACCCTGTCCTCGACCCCTGAGGTCGAGGTGTTGCCAGGCTTCAAGAAGATTCAGCCGCAGGTCTACGCCGGCCTGTTCCCGGTCAGCTCCGACGACTTCGAGGATTTCCGCGACGCTCTGCAGAAGCTGa                                     <  2:2143748/150‑1 (MQ=255)

                                                                                                                   cccGGTTGGTGACACCCTGACCCTGTCCTCGACCCCTGAGGTCGAGGTGTTGCCAGGCTTCAAGAAGATTCAGCCGCAGGTCTACGCCGGCCTGTTCCCGGTCAGCTCCGACGACTTCGAGGATTTCCGCGACGCTCTGCAGAAGCTGAc                                    >  2:5240327/1‑150 (MQ=255)

                                                                                                                   cccGGTTGGTGACACCCTGACCCTGTCCTCGACCCCTGAGGTCGAGGTGTTGCCAGGCTTCAAGAAGATTCAGCCGCAGGTCTACGCCGGCCTGTTCCCGGTCAGCTCCGACGACTTCGAGGATTTCCGCGACGCTCTGCAGAAGCTGAc                                    >  2:295851/1‑150 (MQ=255)

                                                                                                                       gTTGGTGACACCCTGACCCTGTCCTCGACCCCTGAGGTCGAGGTGTTGCCAGGCTTCAAGAAGATTCAGCCGCAGGTCTACGCCGGCCTGTTCCCGGTCAGCTCCGACGACTTCGAGGATTTCCGCGACGCTCTGc                                              <  1:4550816/136‑1 (MQ=255)

                                                                                                                       gTTGGTGACACCCTGACCCTGTCCTCGACCCCTGAGGTCGAGGTGTTGCCAGGCTTCAAGAAGATTCAGCCGCAGGTCTACGCCGGCCTGTTCCCGGTCAGCTCCGACGACTTCGAGGATTTCCGCGACGCTCTGc                                              >  2:4550816/1‑136 (MQ=255)

                                                                                                                          ggTGACACCCTGACCCTGTCCTCGACCCCTGAGGTCGAGGTGTTGCCAGGCTTCAAGAAGATTCAGCCGCAGGTCTACGCCGGCCTGTTCCCGGTCAGCTCCGACGACTTCGAGGATTTCCGCGACGCTCTGCAGAAGCTGACCCTGAAc                             >  2:2530303/1‑150 (MQ=255)

                                                                                                                            tGACACCCTGACCCTGTCCTCGACCCCTGAGGTCGAGGTGTTGCCAGGCTTCAAGAAGATTCAGCCGCAGGTCTACGCCGGCCTGTTCCCGGTCAGCTCCGACGACTTCGAg                                                                 >  2:1401624/1‑112 (MQ=255)

                                                                                                                            tGACACCCTGACCCTGTCCTCGACCCCTGAGGTCGAGGTGTTGCCAGGCTTCAAGAAGATTCAGCCGCAGGTCTACGCCGGCCTGTTCCCGGTCAGCTCCGACGACTTCGAg                                                                 <  1:1401624/112‑1 (MQ=255)

                                                                                                                                accctgaccctgTCCTCGACCCCTGAGGTCGAGGTGTTGCCAGGCTTCAAGAAGATTCAGCCGCAGGTCTACGCCGGCCTGTTCCCGGTCAGCTCCGACGACTTCGAGGATTTCCGCGACGCTCTGCAGAAGCTGACCCTGAACGActcc                       >  2:5386075/1‑150 (MQ=255)

                                                                                                                                  cctgaccctgTCCTCGACCCCTGAGGTCGAGGTGTTGCCAGGCTTCAAGAAGATTCAGCCGCAGGTCTACGCCGGCCTGTTCCCGGTCAGCTCCGACGACTTCGAGGATTTCCGCGACGCTCTGCAGAAGCTGACCCTGAACGActcctc                     >  2:855354/1‑150 (MQ=255)

                                                                                                                                  cctgaccctgTCCTCGACCCCTGAGGTCGAGGTGTTGCCAGGCTTCAAGAAGATTCAGCCGCAGGTCTACGCCGGCCTGTTCCCGGTCAGCTCCGACGACTTCGAGGATTTCCGCGACGCTCTGCAGAAGCTGACCCTGAACGActcctc                     >  2:4094655/1‑150 (MQ=255)

                                                                                                                                  cctgaccctgTCCTCGACCCCTGAGGTCGAGGTGTTGCCAGGCTTCAAGAAGATTCAGCCGCAGGTCTACGCCGGCCTGTTCCCGGTCAGCTCCGACGACTTCGAGGATTTCCGCGACGCTCTGCAGAAGCTGACCCTGAACGActcctc                     >  1:2052199/1‑150 (MQ=255)

                                                                                                                                  cctgaccctgTCCTCGACCCCTGAGGTCGAGGTGTTGCCAGGCTTCAAGAAGATTCAGCCGCAGGTCTACGCCGGCCTGTTCCCGGTCAGCTCCGACGACTTCGAGGATTTCCGCGACGCTCTGCAGAAGCTGACCCTGAACGActcctc                     >  1:2339967/1‑150 (MQ=255)

                                                                                                                                  cctgaccctgTCCTCGACCCCTGAGGTCGAGGTGTTGCCAGGCTTCAAGAAGATTCAGCCGCAGGTCTACGCCGGCCTGTTCCCGGTCAGCTCCGACGACTTCGAGGATTTCCGCGACGCTCTGCAGAAGCTGACCCTGAACGActcctc                     >  1:3946199/1‑150 (MQ=255)

                                                                                                                                   ctgaccctgTCCTCGACCCCTGAGGTCGAGGTGTTGCCAGGCTTCAAGAAGATTCAGCCGCAGGTCTACGCCGGCCTGTTCCCGGTCAGCTCCGACGACTTCGAGGATTTCCGCGACGCTCTGCAGAAGCTGACCCTGAACGACTCCTCg                    <  2:1670372/150‑1 (MQ=255)

                                                                                                                                     gaccctgTCCTCGACCCCTGAGGTCGAGGTGTTGCCAGGCTTCAAGAAGATTCAGCCGCAGGTCTACGCCGGCCTGTTCCCGGTCAGCTCCGACGACTTCGa                                                                  <  2:4342679/102‑1 (MQ=255)

                                                                                                                                     gaccctgTCCTCGACCCCTGAGGTCGAGGTGTTGCCAGGCTTCAAGAAGATTCAGCCGCAGGTCTACGCCGGCCTGTTCCCGGTCAGCTCCGACGACTTCGa                                                                  >  1:4342679/1‑102 (MQ=255)

                                                                                                                                      accctgTCCTCGACCCCTGAGGTCGAGGTGTTGCCAGGCTTCAAGAAGATTCAGCCGCAGGTCTACGCCGGCCTGTTCCCGGTCAGCTCCGACGACTTCGAGGATTTCCGCGACGCTCTGCAGAAGCTGACCCTGAACGACTCCTCGCTg                 <  1:3858018/150‑1 (MQ=255)

                                                                                                                                        cctgTCCTCGACCCCTGAGGTCGAGGTGTTGCCAGGCTTCAAGAAGATTCAGCCGCAGGTCTACGCCGGCCTGTTCCCGGTCAGCTCCGACGACTTCGAGGATTTCCGCGACGCTCTGCAGAAGCTGACCCTGAACGACTCCTCGCTGCa               >  2:3983666/1‑150 (MQ=255)

                                                                                                                                        caacaccTCGACCTCAGGGGTCGAGGTGTTGCCAGGCTTCAAGAAGATTCAGCCGCAGGTCTACGCCGGCCTGTTCCCGGTCAGCTCCGACGACTTCGAGGATTTCCGCGACGCTCTGCAGAAGCTGACCCTGAACGACTCCTCGCTGCa               >  2:3934412/6‑150 (MQ=255)

                                                                                                                                        caacaccTCGACCTCAGGGGTCGAGGTGTTGCCAGGCTTCAAGAAGATTCAGCCGCAGGTCTACGCCGGCCTGTTCCCGGTCAGCTCCGACGACTTCGAGGATTTCCGCGACGCTCTGCAGAAGCTGACCCTGAACGACTCCTCGCTGCa               >  2:16601/6‑150 (MQ=255)

                                                                                                                                         aacaccTCGACCTCAGGGGTCGAGGTGTTGCCAGGCTTCAAGAAGATTCAGCCGCAGGTCTACGCCGGCCTGTTCCCGGTCAGCTcc                                                                             <  1:1932353/83‑1 (MQ=38)

                                                                                                                                          tgTCCTCGACCCCTGAGGTCGAGGTGTTGCCAGGCTTCAAGAAGATTCAGCCGCAGGTCTACGCCGGCCTGTTCCCGGTCAGCTCCGACGACTTCGAGGATTTCCGCGACGCTCTGCAGAAGCTGACCCTGAACGACTCCTCGCTGCAGt             >  1:5075078/1‑150 (MQ=255)

                                                                                                                                            accTCGACCTCAGGGGTCGAGGTGTTGCCAGGCTTCAAGAAGATTCAGCCGCAGGTCTACGCCGGCCTGTTCCCGGTCAGCTCCGACGACTTCGAGGATTTCCGCGACGCTCTGCAGAAGCTGACCCTGAACGACTCCTCGCTGCAGTAc           >  1:5318223/2‑150 (MQ=255)

                                                                                                                                             ccTCGACCTCAGGGGTCGAGGTGTTGCCAGGCTTCAAGAAGATTCAGCCGCAGGTCTACGCCGGCCTGTTCCCGGTCAGCTCCGACGACTTCGAGGATTTCCGCGACGCTCTGCAGAAGCTGACCCTGAACGACTCCTCGCTGCAGTACa          >  1:2366437/1‑150 (MQ=255)

                                                                                                                                              cTCGACCTCAGGGGTCGAGGTGTTGCCAGGCTTCAAGAAGATTCAGCCGCAGGTCTACGCCGGCCTGTTCCCGGTCAGCTCCGACGACTTCGAGGATTTCCGCGACGCTCTGCAGAAGCTGACCCTGAACGACTCCTCGCTGCAGTACAt         >  1:3618630/1‑150 (MQ=255)

                                                                                                                                              cTCGACCTCAGGGGTCGAGGTGTTGCCAGGCTTCAAGAAGATTCAGCCGCAGGTCTACGCCGGCCTGTTCCCGGTCAGCTCCGACGACTTCGAGGATTTCCGCGACGCTCTGCAGAAGCTGACCCTGAACGACTCCTCGCTGCAGTACAt         >  2:3193627/1‑150 (MQ=255)

                                                                                                                                               tCGACCCCTGAGGTCGAGGTGTTGCCAGGCTTCAAGAAGATTCAGCCGCAGGTCTACGCCGGCCTGTTCCCGGTCAGCTCCGACGACTTCGAGGATTTCCGCGACGCTCTGCAGAAGCTGACCCTGAACGACTCCTCGCTGCAGt             <  1:5035250/145‑1 (MQ=255)

                                                                                                                                               tCGACCCCTGAGGTCGAGGTGTTGCCAGGCTTCAAGAAGATTCAGCCGCAGGTCTACGCCGGCCTGTTCCCGGTCAGCTCCGACGACTTCGAGGATTTCCGCGACGCTCTGCAGAAGCTGACCCTGAACGACTCCTCGCTGCAGt             >  2:5035250/1‑145 (MQ=255)

                                                                                                                                               tCGACCCCTGAGGTCGAGGTGTTGCCAGGCTTCAAGAAGATTCAGCCGCAGGTCTACGCCGGCCTGTTCCCGGTCAGCTCCGACGACTTCGAGGATTTCCGCGACGCTCTGCAGAAGCTGACCCTGAACGACTCCTCGCTGCAGTACATg        >  1:4569453/1‑150 (MQ=255)

                                                                                                                                                cGACCCCTGAGGTCGAGGTGTTGCCAGGCTTCAAGAAGATTCAGCCGCAGGTCTACGCCGGCCTGTTCCCGGTCAGCTCCGACGACTTCGAGGATTTCCGCGACGCTCTGCAGAAGCTGACCCTGAACGACTCCTCGCTGCAGTACATGc       <  1:295851/150‑1 (MQ=255)

                                                                                                                                                 gACCCCTGAGGTCGAGGTGTTGCCAGGCTTCAAGAAGATTCAGCCGCAGGTCTACGCCGGCCTGTTCCCGGTCAGCTCCGACGACTTCGAGGATTTCCGCGACGCTCTGCAGAAGCTGACCCTGAACGACTCCTCGCTGCAGTACATGcg      >  2:5089278/1‑149 (MQ=255)

                                                                                                                                                 gACCCCTGAGGTCGAGGTGTTGCCAGGCTTCAAGAAGATTCAGCCGCAGGTCTACGCCGGCCTGTTCCCGGTCAGCTCCGACGACTTCGAGGATTTCCGCGACGCTCTGCAGAAGCTGACCCTGAACGACTCCTCGCTGCAGTACATGcc      >  1:1084120/1‑150 (MQ=255)

                                                                                                                                                  aCCCCTGAGGTCGAGGTGTTGCCAGGCTTCAAGAAGATTCAGCCGCAGGTCTACGCCGGCCTGTTCCCGGTCAGCTCCGACGACTTCGAGGATTTCCGCGACGCTCTGCAGAAGCTGACCCTGAACGACTCCTCGCTGCAGTACATGCCg     <  1:3169433/150‑1 (MQ=255)

                                                                                                                                                     ccTGAGGTCGAGGTGTTGCCAGGCTTCAAGAAGATTCAGCCGCAGGTCTACGCCGGCCTGTTCCCGGTCAGCTCCGACGACGTCGAGGATTTCCGCGACGCTCTGCAGAAGCTGACCCTGAACGACTCCTCGCTGCAGTACATGCCGGaa  >  2:2463540/1‑150 (MQ=255)

AGGTGCACTTGGTCGACAGCGTCGGTGTCTTCACCCCGAAGCACACCCAGACCGCTGATCTGAAAGCCGGCGAAGTAGGCTTCATCATCGCCAGCATCAAGGACATCCACGGTGCCCCGGTTGGTGACACCCTGACCCTGTCCTCGACCCCTGAGGTCGAGGTGTTGCCAGGCTTCAAGAAGATTCAGCCGCAGGTCTACGCCGGCCTGTTCCCGGTCAGCTCCGACGACTTCGAGGATTTCCGCGACGCTCTGCAGAAGCTGACCCTGAACGACTCCTCGCTGCAGTACATGCCGGAA  >  NC_002947/1631986‑1632284

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`