BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	1,784,073	C→G	5.9%	A16P (GCG→CCG) ‡	glnD ←	uridylyltransferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	1,784,073	0	C	G	5.9%	565.4 / 17.6	170	A16P (GCG→CCG) ‡	glnD	uridylyltransferase
Reads supporting (aligned to +/- strand): ref base C (85/75); new base G (4/6); total (89/81)
Fisher's exact test for biased strand distribution p-value = 5.21e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.97e-01

Only 100 of 173 total aligned reads displayed.

TTGCAGGATATTGTCGACGAGCCAGGCGCGGGCCTCGATCAGCGGTCGGATGTCGCTGCCGGCGCGAAAACGTTTGTCGAGCACCTCGCCGGCCAGGCGGATGGCTTTCTTGAAGGCGGCGATGGGGCTCGCCTTGAGGGCCAGTTCCGCCTGGAACTGGCCACGGTCGAACAGCTCGGGGTCCACCTGGGGCATCGCGTCACGTTCCTGTCGTGGGGTGGCCTGTCGGGGTGCGGTCAGGCCGAAGTGCGCGGGATGGTGTCGTCCTTGCGCAGGGTGAAGATCTCGTAGCCAGTG  >  NC_002947/1783924‑1784220

ttGCAGGATATTGTCGACGAGCCAGGCGCGGGCCTCGATCAGCGGTCGGATGTCGCTGCCGGCGCGAAAACGTTTGTCGAGCACCTCGCCGGCCAGGCGGATGGCTTTCTTGAAGGCGGCGATGGGGCTCGCCTTGAGGGCCAGTTCCGc                                                                                                                                                     <  2:2858254/150‑1 (MQ=255)

  gCAGGATATTGTCGACGAGCCAGGCGCGGGCCTCGATCAGCGGTCGGATGTCGCTGCCGGCGCGAAAACGTTTGTCGAGCACCTCGCCGGCCAGGCGGATGGCTTTCTTGAAGGCGGCGATGGGGCTCGCCTTGAGGGCCAGTTCCGCCt                                                                                                                                                   >  1:4595062/1‑150 (MQ=255)

    aGGATATTGTCGACGAGCCAGGCGCGGGCCTCGATCAGCGGTCGGATGTCGCTGCCGGCGCGAAAACGTTTGTCGAGCACCTCGCCGGCCAGGCGGATGGCTTTCTTGAAGGCGGCGATGGGGCTCGCCTTGAGGGCCAGTTCCGCCTgg                                                                                                                                                 >  2:3822104/1‑150 (MQ=255)

         atTGTCGACGAGCCATGCGCGGGCCTCGATCAGCGGTCGGATGTCGCTGCCGGCGCGAAAACGTTTGTCGAGCACCTCGCCGGCCAGGCGGATGGCTTTCTTGAAGGCGGCGATGGGGCTCGCCTTGAGGGCCAGTTCCGCCTGGAACTg                                                                                                                                            <  2:1800290/150‑1 (MQ=255)

         atTGTCGACGAGCCAGGCGCGGGCCTCGATCAGCGGTCGGATGTCGCTGCCGGCGCGAAAACGTTTGTCGAGCACCTCGCCGGCCAGGCGGATGGCTTTCTTGAAGGCGGCGATGGGGCTCGCCTTGAGGGCCAGTTCCGCCTGGAACTg                                                                                                                                            <  2:652325/150‑1 (MQ=255)

          ttGTCGACGAGCCAGGCGCGGGCCTCGATCAGCGGTCGGATGTCGCTGCCGGCGCGAAAACGTTTGTCGAGCACCTCGCCGGCCAGGCGGATGGCTTTCTTGAAGGCGGCGATGGGGCTCGCCTTGAGGGCCAGTTCCAGGCGGAACTgg                                                                                                                                           <  2:1138049/150‑1 (MQ=255)

            gTCGACGAGCCAGGCGCGGGCCTCGATCAGCGGTCGGATGTCGCTGCCGGCGCGAAAACGTTTGTCGAGCACCTCGCCGGCCAGGCGGATGGCTTTCTTGAAGGCGGCGATGGGGCTCGCCTTGAGGGCCAGTTCCGCCTGGAACTGGcc                                                                                                                                         >  1:5319745/1‑150 (MQ=255)

             tCGACGAGCCAGGCGCGGGCCTCGATCAGCGGTCGGATGTCGCTGCCGGCGCGAAAACGTTTGTCGAGCACCTCGCCGGCCAGGCGGATGGCTTTCTTGAAGGCGGCGATGGGGCTCGCCTTGAGGGCCAGTTCCGCCTGGAACTGGCCa                                                                                                                                        >  1:4239439/1‑150 (MQ=255)

                acgaGCCAGGCGCGGGCCTCGATCAGCGGTCGGATGTCGCTGCCGGCGCGAAAACGTTTGTCGAGCACCTCGCCGGCCAGGCGGATGGCTTTCTTGAAGGCGGCGATGGGGCTCGCCTTGAGGGCCAGTTCCGCCTGGAACTGGCCACgg                                                                                                                                     >  1:4876026/1‑150 (MQ=255)

                  gaGCCAGGCGCGGGCCTCGATCAGCGGTCGGATGTCGCTGCCGGCGCGAAAACGTTTGTCGAGCACCTCGCCGGCCAGGCGGATGGCTTTCTTGAAGGCGGCGATGGGGCTCGCCTTGAGGGCCAGTTCCGCCTGGAACTGGCCACGGTc                                                                                                                                   <  1:5370790/150‑1 (MQ=255)

                   aGCCAGGCGCGGGCCTCGATCAGCGGTCGGATGTCGCTGCCGGCGCGAAAACGTTTGTCGAGCACCTCGCCGGCCAGGCGGATGGCTTTCTTGAAGGCGGCGATGGGGCTCGCCTTGAGGGCCAGTTCCGCCTGGAACTGGCCACGGTCg                                                                                                                                  >  2:4018439/1‑150 (MQ=255)

                    gCCAGGCGCGGGCCTCGATCAGCGGTCGGATGTCGCTGCCGGCGCGAAAACGTTTGTCGAGCACCTCGCCGGCCAGGCGGATGGCTTTCTTGAAGGCGGCGATGGGGCTCGCCTTGAGGGCCAGTTCCGCCTGGAACTGGCCACGGTCGa                                                                                                                                 >  2:2606417/1‑150 (MQ=255)

                     ccAGGCGCGGGCCTCGATCAGCGGTCGGATGTCGCTGCCGGCGCGAAACCGTTTGTCGAGCACCTCGCCGGCCAGGCGGATGGCTTTCTTGAAGGCGGCGATGGGGCTCGCCTTGAGGGCCAGTTCCGCCTGGAACTGGCCACGGTCGaa                                                                                                                                <  2:5419522/150‑1 (MQ=255)

                     ccAGGCGCGGGCCTCGATCAGCGGTCGGATGTCGCTGCCGGCGCGAAAACGTTTGTCGAGCACCTCGCCGGCCAGGCGGATGGCTTTCTTGAAGGCGGCGATGGGGCTCGCCTTGAGGGCCAGTTCCGCCTGGAACTGGCCACGGTCGaa                                                                                                                                <  2:4436573/150‑1 (MQ=255)

                       aGGCGCGGGCCTCGATCAGCGGTCGGATGTCGCTGCCGGCGCGAAAACGTTTGTCGAGCACCTCGCCGGCCAGGCGGATGGCTTTCTTGAAGGCGGCGATGGGGCTCGCCTTGAGGGCCAGTTCCGCCTGGAACTGGCCACGGTCGAACa                                                                                                                              >  2:3702552/1‑150 (MQ=255)

                            cgGGCCTCGATCAGCGGTCGGATGTCGCTGCCGGCGCGAAAACGTTTGTCGAGCACCTCGCCGGCCAGGCGGATGGCTTTCTTGAAGGCGGCGATGGGGCTCGCCTTGAGGGCCAGTTCCGCCTGGAACTGGCCACGGTCGAACAGCTCg                                                                                                                         <  2:3218770/150‑1 (MQ=255)

                             gggCCTCGATCAGCGGTCGGATGTCGCTGCCGGCGCGAAAACGTTTGTCGAGCACCTCGCCGGCCAGGCGGATGGCTTTCTTGAAGGCGGCGATGGGGCTCGCCTTGAGGGCCAGTTCCGCCTGGAACTGGCCACGGTCGAACAGCTCgg                                                                                                                        >  1:4357544/1‑150 (MQ=255)

                                 cTCGATCAGCGGTCGGATGTCGCTGCCGGCGCGAAAACGTTTGTCGAGCACCTCGCCGGCCAGGCGGATGGCTTTCTTGAAGGCGGCGATGGGGCTCGCCTTGAGGGCCAGTTCCGCCTGGAACTGGCCACGGTCGAACAGCTCGGGGTc                                                                                                                    >  1:2887220/1‑150 (MQ=255)

                                 cTCGATCAGCGGTCGGATGTCGCTGCCGGCGCGAAAACGTTTGTCGAGCACCTCGCCGGCCAGGCGGATGGCTTTCTTGAAGGCGGCGATGGGGCTCGCCTTGAGGGCCAGTTCCGCCTGGAACTGGCCACGGTCGAACAGCTCGGGGTc                                                                                                                    >  1:2795659/1‑150 (MQ=255)

                                 cTCGATCAGCGGTCGGATGTCGCTGCCGGCGCGAAAACGTTTGTCGAGCACCTCGCCGGCCAGGCGGATGGCTTTCTTGAAGGCGGCGATGGGGCTCGCCTTGAGGGCCAGTTCCGCCTGGAACTGGCCACGGTCGAACAGCTCGGGGTc                                                                                                                    >  2:2011098/1‑150 (MQ=255)

                                    gATCAGCGGTCGGATGTCGCTGCCGGCGCGAAAACGTTTGTCGAGCACCTCGCCGGCCAGGCGGATGGCTGTCTTGAAGGCGGCGATGGGGCTCGCCTTGAGGGCCAGTTCCGCCTGGAACTGGCCACGGTCGAACAGCTCGGGGTCCAc                                                                                                                 >  2:1333133/1‑150 (MQ=255)

                                      tCAGCGGTCGGATGTCGCTGCCGGCGCGAAAACGTTTGTCGAGCACCTCGCCGGCCAGGCGGATGGCTTTCTTGAAGGCGGCGATGGGGCTCGCCTTGAGGGCCAGTTCCGCCTGGAACTGGCCACGGTCGAACAGCTCGGGGTCCACCt                                                                                                               <  1:3717487/150‑1 (MQ=255)

                                       cAGCGGTGGGTTTTCGCTGCCGGCGCGAAAACGTTTGGCGAGCACCTCGGCGGCCAGGCGGATGGCTTTCTTGAAGGCGGCGATGGGGCTCGCCTTGAGGGCCAGTTCCGCCTGGAACTGGCCACGGTCGAACAGCTCGGGGTCCACCTg                                                                                                              <  1:1380797/150‑1 (MQ=255)

                                        aGCGGTCGGATGTCGCTGCCGGCGCGAAAACGTTTGTCGAGCACCTCGCCGGCCAGGCGGATGGCTTTCTTGAAGGCGGCGATGGGGCTCGCCTTGAGGGCCAGTTCCGCCTGGAACTGGCCACGGTCGAACAGCTCGGGGTCCACCTgg                                                                                                             <  2:3957139/150‑1 (MQ=255)

                                                  tGTCGCTGCCGGCGCGAAAACGTTTGTCGAGCACCTCGCCGGCCAGGCGGATGGCTTTCTTGAAGGCGGCGATGGGGCTCGCCTTGAGGGCCAGTTCCGCCTGGAACTGGCCACGGTCGAACAGCTCGGGGTCCACCTGGGGCATCGCGt                                                                                                   <  2:2927891/150‑1 (MQ=255)

                                                   gTCGCTGCCGGCGCGAAAACGTTTGTCGAGCACCTCGCCGGCCAGGCGGATGGCTTTCTTGAAGGCGGCGATGGGGCTCGCCTTGAGGGCCAGTTCCGCCTGGAACTGGCCACGGTCGAACAGCTCGGGGTCCACCTGGGGCATCGCGTc                                                                                                  <  2:306591/150‑1 (MQ=255)

                                                            ggCGCGAAAACGTTTGTCGAGCACCTCGCCGGCCAGGCGGATGGCTTTCTTGAAGGCGGCGATGGGGCTCGCCTTGAGGGCCAGTTCCGCCTGGAACTGGCCACGGTCGAACAGCTCGGGGTCCACCTGGGGCATCGCGTCACGTTCCTg                                                                                         >  1:3120353/1‑150 (MQ=255)

                                                               gcgAAAACGTTTGTCGAGCACCTCGCCGGCCAGGCGGATGGCTTTCTTGAAGGCGGCGATGGGGCTCGCCTTGAGGGCCAGTTCCGCCTGGAACTGGCCACGGTCGAACAGCTCGGGGTCCACCTGGGGCATCGCGTCACGTTCCTGTCg                                                                                      >  2:3533190/1‑150 (MQ=255)

                                                                cgAAAACGTTTGTCGAGCACCTCGCCGGCCAGGCGGATGGCTTTCTTGAAGGCGGCGATGGGGCTCGCCTTGAGGGCCAGTTCCGCCTGGAACTGGCCACGGTCGAACAGCTCGGGGTCCACCTGGGGCATCGCGTCACGTTCCTGTCgg                                                                                     >  2:3831469/1‑149 (MQ=255)

                                                                   aaaCGTTTGTCGAGCACCTCGCCGGCCAGGCGGATGGCTTTCTTGAAGGCGGCGATGGGGCTCGCCTTGAGGGCCAGTTCCGCCTGGAACTGGCCACGGTCGAACAGCTCGGGGTCCACCTGGGGCATCGCGTCACGTTCCTGTCGTggg                                                                                  >  1:685999/1‑150 (MQ=255)

                                                                      cGTTTGTCGAGCACCTCGCCGGCCAGGCGGATGGCTTTCTTGAAGGCGGCGATGGGGCTCGCCTTGAGGGCCAGTTCCGCCTGGAACTGGCCACGGTCGAACAGCTCGGGGTCCACCTGGGGCATCGCGTCACGTTCCTGTCGTGGGGTg                                                                               <  2:4449141/150‑1 (MQ=255)

                                                                       gTTTGTCGAGCACCTCGCCGGCCAGGCGGATGGCTTTCTTGAAGGCGGCGATGGGGCTCGCCTTGAGGGCCAGTTCCGCCTGGAACTGGCCACGGTCGAACAGCTCGGGGTCCACCTGGGGCATCGCGTCACGTTCCTGTCGTGGGGTgg                                                                              >  1:608893/1‑150 (MQ=255)

                                                                       gTTTGTCGAGCACCTCGCCGGCCAGGCGGATGGCTTTCTTGAAGGCGGCGATGGGGCTCGCCTTGAGGGCCAGTTCCGCCTGGAACTGGCCACGGTCGAACAGCTCGGGGTCCACCTGGGGCATCGCGTCACGTTCCTGTCGTGGGGTgg                                                                              >  1:3198693/1‑150 (MQ=255)

                                                                       gTTTGTCGAGCACCTCGCCGGCCAGGCGGATGGCTTTCTTGAAGGCGGCGATGGGGCTCGCCTTGAGGGCCAGTTCCGCCTGGAACTGGCCACGGTCGAACAGCTCGGGGTCCACCTGGGGCATCGCGTCACGTTCCTGTCGTGGGGTgg                                                                              >  1:4467396/1‑150 (MQ=255)

                                                                       gTTTGTCGAGCACCTCGCCGGCCAGGCGGATGGCTTTCTTGAAGGCGGCGATGGGGCTCGCCTTGAGGGCCAGTTCCGCCTGGAACTGGCCACGGTCGAACAGCTCGGGGTCCACCTGGGGCATCGCGTCACGTTCCTGTCGTGGGGTgg                                                                              >  1:4979677/1‑150 (MQ=255)

                                                                        tttGTCGAGCACCTCGCCGGCCAGGCGGATGGCTTTCTTGAAGGCGGCGATGGGGCTCGCCTTGAGGGCCAGTTCCGCCTGGAACTGGCCACGGTCGAACAGCTCGGGGTCCACCTGGGGCATCGCGTCACGTTCCTGTCGTGGGGTGGc                                                                             >  1:1809219/1‑150 (MQ=255)

                                                                           gTCGAGCACCTCGCCGGCCAGGCGGATGGCTTTCTTGAAGGCGGCGATGGGGCTCGCCTTGAGGGCCAGTTCCGCCTGGAACTGGCCACGGTCGAACAGCTCGGGGTCCACCTGGGGCATCGCGTCACGTTCCTGTCGTgg                                                                                   >  2:1272229/1‑141 (MQ=255)

                                                                           gTCGAGCACCTCGCCGGCCAGGCGGATGGCTTTCTTGAAGGCGGCGATGGGGCTCGCCTTGAGGGCCAGTTCCGCCTGGAACTGGCCACGGTCGAACAGCTCGGGGTCCACCTGGGGCATCGCGTCACGTTCCTGTCGTgg                                                                                   <  1:1272229/141‑1 (MQ=255)

                                                                              gAGCACCTCGCCGGCCAGGCGGATGGCTTTCTTGAAGGCGGCGATGGGGCTCGCCTTGAGGGCCAGTTCCGCCTGGAACTGGCCACGGTCGAACAGCTCGGGGTCCACCTGGGGCATCGCGTCACGTTCCTGTCGTGGGGTgg                                                                              >  2:2398280/1‑143 (MQ=255)

                                                                              gAGCACCTCGCCGGCCAGGCGGATGGCTTTCTTGAAGGCGGCGATGGGGCTCGCCTTGAGGGCCAGTTCCGCCTGGAACTGGCCACGGTCGAACAGCTCGGGGTCCACCTGGGGCATCGCGTCACGTTCCTGTCGTGGGGTGGCCTGTCg                                                                       <  2:608893/150‑1 (MQ=255)

                                                                              gAGCACCTCGCCGGCCAGGCGGATGGCTTTCTTGAAGGCGGCGATGGGGCTCGCCTTGAGGGCCAGTTCCGCCTGGAACTGGCCACGGTCGAACAGCTCGGGGTCCACCTGGGGCATCGCGTCACGTTCCTGTCGTGGGGTGGCCTGTCg                                                                       >  1:113847/1‑150 (MQ=255)

                                                                              gAGCACCTCGCCGGCCAGGCGGATGGCTTTCTTGAAGGCGGCGATGGGGCTCGCCTTGAGGGCCAGTTCCGCCTGGAACTGGCCACGGTCGAACAGCTCGGGGTCCACCTGGGGCATCGCGTCACGTTCCTGTCGTGGGGTGGCCTGTCg                                                                       >  2:1532159/1‑150 (MQ=255)

                                                                              gAGCACCTCGCCGGCCAGGCGGATGGCTTTCTTGAAGGCGGCGATGGGGCTCGCCTTGAGGGCCAGTTCCGCCTGGAACTGGCCACGGTCGAACAGCTCGGGGTCCACCTGGGGCATCGCGTCACGTTCCTGTCGTGGGGTGGCCTGTCg                                                                       >  2:799286/1‑150 (MQ=255)

                                                                              gAGCACCTCGCCGGCCAGGCGGATGGCTTTCTTGAAGGCGGCGATGGGGCTCGCCTTGAGGGCCAGTTCCAGGCGGAACTGGcc                                                                                                                                         >  1:2099074/1‑84 (MQ=38)

                                                                              gAGCACCTCGCCGGCCAGGCGGATGGCTTTCTTGAAGGCGGCGATGGGGCTCGCCTTGAGGGCCAGTTCCAGGCGGAACTGGcc                                                                                                                                         <  2:2099074/84‑1 (MQ=38)

                                                                                   ccTCGCCGGCCAGGCGGATGGCTTTCTTGAAGGCGGCGATGGGGCTCGCCTTGAGGGCCAGTTCCGCCTGGAACTGGCCACGGTCGAACAGCTCGGGGTCCACCTGGGGCATCGCGTCACGTTCCTGTCGTGGGGTGGCCTGTCGGGGTg                                                                  >  1:1609199/1‑150 (MQ=255)

                                                                                    cTCGCCGGCCAGGCGGATGGCTTTCTTGAAGGCGGCGATGGGGCTCGCCTTGAGGGCCAGTTCCGCCTGGAACTGGCCACGGTCGAACAGCTCGGGGTCCACCTGGGGCATCGCGTCACGTTCCTGTCGTGGGGt                                                                                >  1:5309031/1‑135 (MQ=255)

                                                                                    cTCGCCGGCCAGGCGGATGGCTTTCTTGAAGGCGGCGATGGGGCTCGCCTTGAGGGCCAGTTCCGCCTGGAACTGGCCACGGTCGAACAGCTCGGGGTCCACCTGGGGCATCGCGTCACGTTCCTGTCGTGGGGt                                                                                <  2:5309031/135‑1 (MQ=255)

                                                                                    cTCGCCGGCCAGGCGGATGGCTTTCTTGAAGGCGGCGATGGGGCTCGCCTTGAGGGCCAGTTCCGCCTGGAACTGGCCACGGTCGAACAGCTCGGGGTCCACCTGGGGCATCGCGTCACGTTCCTGTCGTGGGGt                                                                                <  2:5308915/135‑1 (MQ=255)

                                                                                    cTCGCCGGCCAGGCGGATGGCTTTCTTGAAGGCGGCGATGGGGCTCGCCTTGAGGGCCAGTTCCGCCTGGAACTGGCCACGGTCGAACAGCTCGGGGTCCACCTGGGGCATCGCGTCACGTTCCTGTCGTGGGGTGGCCTGTCGGGGTGc                                                                 >  1:5151277/1‑150 (MQ=255)

                                                                                     tCGCCGGCCAGGCGGATGGCTTTCTTGAAGGCGGCGATGGGGCTCGCCTTGAGGGCCAGTTCCGCCTGGAACTGGCCACGGTCGAACAGCTCGGGGTCCACCTGGGGCATCGCGTCACGTTCCTGTCGTGGGGTGGCCTGTCGGGGTGCg                                                                <  1:2609916/150‑1 (MQ=255)

                                                                                     tCGCCGGCCAGGCGGATGGCTTTCTTGAAGGCGGCGATGGGGCTCGCCTTGAGGGCCAGTTCCAGGCGGAACTGGcc                                                                                                                                         <  2:1680273/77‑1 (MQ=38)

                                                                                     tCGCCGGCCAGGCGGATGGCTTTCTTGAAGGCGACGATGGGGCTCGCCTTGAGGGCCAGTTCCGCCTGGAACTGGCCACGGTCGAACAGCTCGGGGTCCACCTGGGGCATCGCGTCACGTTCCTGTCGTGGGGTGGCCTGTCGGGGTGCg                                                                >  1:772342/1‑150 (MQ=255)

                                                                                      cGCCGGCCAGGCGGATGGCTTTCTTGAAGGCGGCGATGGGGCTCGCCTTGAGGGCCAGTTCCGCCTGGAACTGGCCACGGTCGAACAGCTCGGGGTCCACCTGGGGCATCGCGTCACGTTCCTGTCGTGGGGTGGCCTGTCGGGGTGCgg                                                               >  1:4595992/1‑150 (MQ=255)

                                                                                       gCCGGCCAGGCGGATGGCTTTCTTGAAGGCGGCGATGGGGCTCGCCTTGAGGGCCAGTTCCGCCTGGAACTGGCCACGGTCGAACAGCTCGGGGTCCACCTGGGGCATCGCGTCACGTTCCTGTCGTGGGGTGGCCTGTCGGGGTGCGGt                                                              >  2:2259140/1‑150 (MQ=255)

                                                                                          ggCCAGGCGGATGGCTTTCTTGAAGGCGGCGATGGGGCTCGCCTTGAGGGCCAGTTCCGCCTGGAACTGGCCACGGTCGAACAGCTCGGGGTCCACCTGGGGCATCGCGTCACGTTCCTGTCGTGGGGTGGCCTGTCGGGGTGCGGTCAg                                                           <  1:4816313/150‑1 (MQ=255)

                                                                                          ggCCAGGCGGATGGCTTTCTTGAAGGCGGCGATGGGGCTCGCCTTGAGGGCCAGTTCCGCCTGGAACTGGCCACGGTCGAACAGCTCGGGGTCCACCTGGGGCATCGCGTCACGTTCCTGTCGTGGGGTGGCCTGTCGGGGTGCGGTCAg                                                           >  1:2343135/1‑150 (MQ=255)

                                                                                              aGGCGGATGGCTTTCTTGAAGGCGGCGATGGGGCTCGCCTTGAGGGCCAGTTCCGCCTGGAACTGGCCACGGTCGAACAGCTCGGGGTCCACCTGGGGCATCGCGTCACGTTCCTGTCGTGGGGTGGCCTGTCGGGGTGCGGTCAGGCCg                                                       >  2:4174010/1‑150 (MQ=255)

                                                                                                gCGGATGGCTTTCTTGAAGGCGGCGATGGGGCTCGCCTTGAGGGCCAGTTCCGCCTGGAACTGGCCACGGTCGAACAGCTCGGGGTCCACCTGGGGCATCGCGTCACGTTCCTGTCGTGGGGTGGCCTGTCGGGGTGCGGTCAGGCCGaa                                                     <  1:4152568/150‑1 (MQ=255)

                                                                                                gCGGATGGCTTTCTTGAAGGCGGCGATGGGGCTCGCCTTGAGGGCCAGTTCCGCCTGGAACTGGCCACGGTCGAACAGCTCGGGGTCCACCTGGGGCATCGCGTCACGTTCCTGTCGTGGGGTGGCCTGTCGGGGTGCGGTCAGGCCGaa                                                     >  2:4153240/1‑150 (MQ=255)

                                                                                                gCGGATGGCTGTCTTGAGGGCGGCGCTGGGGCTCGCCTTGAGGGCCGGTTGCGCCTGGCACTGGCCACGGTCGACCCGCTCGGGGTCCACCTGGGGCATCGCGTCACGTGCCTGTGGTGGGGTGGCGTGTGGGGGTGCGGTCGGGCCggg                                                     >  2:4153358/1‑148 (MQ=255)

                                                                                                  ggATGGCTTTCTTGAAGGCGGCGATGGGGCTCGCCTTGAGGGCCAGTTCCGCCTGGAACTGGCCACGGTCGAACAGCTCGGGGTCCACCTGGGGCATCGCGTCACGTTCCTGTCGTGGGGTGGCCTGTCGGGGTGCGGTCAGGCCGAAGt                                                   >  2:922627/1‑150 (MQ=255)

                                                                                                   gATGGCTTTCTTGAAGGCGGCGATGGGGCTCGCCTTGAGGGCCAGTTCCGCCTGGAACTGGCCACGGTCGAACAGCTCGGGGTCCACCTGGGGCATCGCGTCACGTTCCTGTCGTGGGGTGGCCTGTCGGGGTGCGGTCAGGCCGAAGTg                                                  >  1:4008157/1‑150 (MQ=255)

                                                                                                      ggCTTTCTTGAAGGCGGCGATGGGGCTCGCCTTGAGGGCCAGTTCCGCCTGGAACTGGCCACGGTCGAACAGCTCGGGGTCCACCTGGGGCATCGCGTCACGTTCCTGTCGTGGGGTGGCCTGTCggg                                                                     >  1:4376558/1‑128 (MQ=255)

                                                                                                      ggCTTTCTTGAAGGCGGCGATGGGGCTCGCCTTGAGGGCCAGTTCCGCCTGGAACTGGCCACGGTCGAACAGCTCGGGGTCCACCTGGGGCATCGCGTCACGTTCCTGTCGTGGGGTGGCCTGTCGGGGTGCGGTCAGGCCGAAGTgcgc                                               <  2:5328155/150‑1 (MQ=255)

                                                                                                         tttCTTGAAGGCGGCGATGGGGCTCGCCTTGAGGGCCAGTTCCGCCTGGAACTGGCCACGGTCGAACAGCTCGGGGTCCACCTGGGGCATCGCGTCACGTTCCTGTCGTGGGGTGGCCTGTCGGGGTGCGGTCAGGCCGAAGTGCGCggg                                            >  2:3863528/1‑150 (MQ=255)

                                                                                                          ttCTTGAAGGCGGCGATGGGGCTCGCCTTGAGGGCCAGTTCCGCCTGGAACTGGCCACGGTCGAACAGCTCGGGGTCCACCTGGGGCATCGCGTCACGTTCCTGTCGTGGGGTGGCCTGTCGGGGTGCGGTCAGGCCGAAGTGCGCGGGa                                           <  1:4094901/150‑1 (MQ=255)

                                                                                                            cTTGAAGGCGGCGATGGGGCTCGCCTTGAGGGCCAGTTCCGCCTGGAACTGGCCACGGTCGAACAGCTCGGGGTCCACCTGGGGCATCGCGTCACGTTCCTGTCGTGGGGTGGCCTGTCGGGGTGCGGTCAGGCCGAAGTGCGCGGGATg                                         <  2:4922134/150‑1 (MQ=255)

                                                                                                            cTTGAAGGCGGCGATGGGGCTCGCCTTGAGGGCCAGTTCCGCCTGGAACTGGCCACGGTCGAACAGCTCGGGGTCCACCTGGGGCATCGCGTCACGTTCCTGTCGTGGGGTGGCCTGTCGGGGTGCGGTCAGGCCGAAGTGCGCGGGATg                                         <  2:4868367/150‑1 (MQ=255)

                                                                                                            cTTGAAGGCGGCGATGGGGCTCGCCTTGAGGGCCAGTTCCGCCTGGAACTGGCCACGGTCGAACAGCTCGGGGTCCACCTGGGGCATCGCGTCACGTTCCTGTCGTGGGGTGGCCTGTCGGGGTGCGGTCAGGCCGAAGTGCGCGGGATg                                         <  1:3863528/150‑1 (MQ=255)

                                                                                                            cTTGAAGGCGGCGATGGGGCTCGCCTTGAGGGCCAGGTCCGCCTGGAACTGGCCACGGTCGAACAGCTCGGGGTCCACCTGGGGCATCGCGTCACGTTCCTGTCGTGGGGTGGCCTGTCGGGGTGCGGTCAGGCCGAAGTGCGCGGGATg                                         <  2:4868124/150‑1 (MQ=255)

                                                                                                             ttGAAGGCGGCGATGGGGCTCGCCTTGAGGGCCAGTTCCGCCTGGAACTGGCCACGGTCGAACAGCTCGGGGTCCACCTGGGGCATCGCGTCACGTTCCTGTCGTGGGGTGGCCTGTCGGGGTGCGGTCAGGCCGAAGTGCGCGGGATgg                                        >  2:4636257/1‑150 (MQ=255)

                                                                                                             ttGAAGGCGGCGATGGGGCTCGCCTTGAGGGCCAGTTCCGCCTGGAACTGGCCACGGTCGAACAGCTCGGGGTCCACCTGGGGCATCGCGTCACGTTCCTGTCGTGGGGTGGCCTGTCGGGGTGCGGTCAGGCCGAAGTGCGCGGGATgg                                        >  2:3609413/1‑150 (MQ=255)

                                                                                                              tGAAGGCGGCGATGGGGCTCGCCTTGAGGGCCAGTTCCGCCTGGAACTGGCCACGGTCGAACAGCTCGGGGTCCACCTGGGGCATCGCGTCACGTTCCTGTCGTGGGGTGGCCTGTCGGGGTGCGGTCAGGCCGAAGTGCGCGGGATGgt                                       >  2:2688008/1‑150 (MQ=255)

                                                                                                                     ggcgATGGGGCTCGCCTTGAGGGCCAGTTCCGCCTGGAACTGGCCACGGTCGAACAGCTCGGGGTCCACCTGGGGCATCGCGTCACGTTCCTGTCGTGGGGTGGCCTGTCGGGGTGCGGTCAGGCCGAAGTGCGCGGGATGGTGTCGTcc                                >  2:3891140/1‑150 (MQ=255)

                                                                                                                       cgATGGGGCTCGGCTTGAGGGCCAGTTCCGCGTGGAACTGGCCACGGTCGAACAGCTCGGGGTCGACCTGGGGCTTCGCGTCTCGTTCCTGTCGTGGGGTGGCCTGTCGGGGTGCGGTCAGGCCGAAGTGCGCGGGATGGTGTGGTCCtt                              <  1:1165398/150‑1 (MQ=255)

                                                                                                                       cgATGGGGCTCGCCTTGAGGGCCAGTTCCGCCTGGAACTGGCCACGGTCGAACAGCTCGGGGTCCACCTGGGGCATCGCGTCACGTTCCTGTCGTGGGGTGGCCTGTCGGGGTGCGGTCAGGCCGAAGTGCGCGGGATGGTGTCGTCCtt                              <  2:3388065/150‑1 (MQ=255)

                                                                                                                              gCTCGCCTTGAGGGCCAGTTCCGCCTGGAACTGGCCACGGTCGAACAGCTCGGGGTCCACCTGGGGCATCGCGTCACGTTCCTGTCGTGGGGTGGCCTGTCGGGGTGCGGTCTGGCCGAAGTGCGCGGGATGGTTTCGTCCTTGCGCAgg                       <  2:4538086/150‑1 (MQ=255)

                                                                                                                              gCTCGCCTTGAGGGCCAGTTCCGCCTGGAACTGGCCACGGTCGAACAGCTCGGGGTCCACCTGGGGCATCGCGTCACGTTCCTGTCGTGGGGTGGCCTGTCGGGGTGCGGTCAGGCCGAAGTGCGCGGGATGGTGTCGTCCTTGCGCAgg                       <  1:2011098/150‑1 (MQ=255)

                                                                                                                              gCTCGCCTTGAGGGCCAGTTCCGCCTGGAACTGGCCACGGTCGAACAGCTCGGGGTCCACCTGGGGCATCGCGTCACGTTCCTGTCGTGGGGTGGCCTGTCGGGGTGCGGTCAGGCCGAAGTGCGCGGGATGGTGTCGTCCTTGCGCAgg                       <  2:2660201/150‑1 (MQ=255)

                                                                                                                              gCTCGCCTTGAGGGCCAGTTCCGCCTGGAACTGGCCACGGTCGAACAGCTCGGGGTCCACCTGGGGCATCGCGTCACGTTCCTGTCGTGGGGTGGCCTGTCGGGGTGCGGTCAGGCCGAAGTGCGCGGGATGGTGTCGTCCTTGCGCAgg                       <  2:5032562/150‑1 (MQ=255)

                                                                                                                               cTCGCCTTGAGGGCCAGTTCCGCCTGGAACTGGCCACGGTCGAACAGCTCGGGGTCCACCTGGGGCATCGCGTCACGTTCCTGTCGTGGGGt                                                                                >  1:2983635/1‑92 (MQ=255)

                                                                                                                               cTCGCCTTGAGGGCCAGTTCCGCCTGGAACTGGCCACGGTCGAACAGCTCGGGGTCCACCTGGGGCATCGCGTCACGTTCCTGTCGTGGGGt                                                                                <  2:2983635/92‑1 (MQ=255)

                                                                                                                                tCGCCTTGAGGGCCAGTTCCGCCTGGAACTGGCCACGGTCGAACAGCTCGGGGTCCACCTGGGGCATCGCGTCACGTTCCTGTCGTGGGGTGGCCTGTCGGGGTGCGGTCAGGCCGAAGTGCGCGGGATGGTGTCGTCCTTGCGCAGGGt                     >  1:919024/1‑150 (MQ=255)

                                                                                                                                  gCCTTGAGGGCCAGTTCCGCCTGGAACTGGCCACGGTCGAACAGCTCGGGGTCCACCTGGGGCATCGCGTCACGTTCCTGTCGTGGGGTGGCCTGTCGGGGTGCGGTCAGGCCGAAGTGCGCGGGATGGTGTCGTCCTTGCGCAGGGTGa                   >  1:3844526/1‑150 (MQ=255)

                                                                                                                                  gCCTTGAGGGCCAGTTCCGCCTGGAACTGGCCACGGTCGAACAGCTCGGGGTCCACCTGGGGCATCGCGTCACGTTCCTGTCGTGGGGTGGCCTGTCGGGGTGCGGTCAGGCCGAAGTGCGCGGGATGGTGTCGTCCTTGCGCAGGGTGa                   >  1:3844516/1‑150 (MQ=255)

                                                                                                                                  gCCTTGAGGGCCAGTTCCGCCTGGAACTGGCCACGGTCGAACAGCTCGGGGTCCACCTGGGGCATCGCGTCACGTTCCTGTCGTGGGGTGGCCTGTCGGGGTGCGGTCAGGCCGAAGTGCGCGGGATGGTGTCGTCCTTGCGCAGGGTGa                   <  1:3633463/150‑1 (MQ=255)

                                                                                                                                   ccTTGAGGGCCAGTTCCGCCTGGAACTGGCCACGGTCGAACAGCTCGGGGTCCACCTGGGGCATCGCGTCACGTTCCTGTCGTGGGGTGGCCTGTCGGGGTGCGGTCAGGCCGAAGTGCGCGGGATGGTGTCGTCCTTGCGCAGGGTGaa                  >  1:1145244/1‑150 (MQ=255)

                                                                                                                                   ccTTGAGGGCCAGTTCCGCCTGGAACTGGCCACGGTCGAACAGCTCGGGGTCCACCTGGGGCATCGCGTCACGTTCCTGTCGTGGGGTGGCCTGTCGGGGTGCGGTCAGGCCGAAGTGCGCGGGATGGTGTCGTCCTTGCGCAGGGTGaa                  <  1:3914009/150‑1 (MQ=255)

                                                                                                                                      tGAGGGCCAGTTCCGCCTGGAACTGGCCACGGTCGAACAGCTCGGGGTCCACCTGGGGCATCGCGTCACGTTCCTGTCGTGGGGTGGCCTGTCGGGGTGCGGTCAGGCCGAAGTGCGCGGGATGGTGTCGTCCTTGCGCAGGGTGAAGAt               <  2:2543099/150‑1 (MQ=255)

                                                                                                                                       cgtggCCAGTTCCAGGCGGAACTGGCCACGGTCGAACAGCTCGGGGTCCACCTGGGGCATCGCGTCACGTTCCTGTCGTGGGGTGGCCTGTCGGGGTGCGGTCAGGCCGAAGTGCGCGGGATGGTGTCGTCCTTGCGCAGGGTGAAGAtc              >  1:338306/4‑150 (MQ=255)

                                                                                                                                         gggCCAGTTCCGCCTGGAACTGGCCACGGTCGAACAGCTCGGGGTCCACCTGGGGCATCGCGTCACGTTCCTGTCGTGGGGTGGCCTGTCGGGGTGCGGTCAGGCCGAAGTGCGCGGGATGGTGTCGTCCTTGCGCAGGGTGAAGAtctc            <  1:799286/150‑1 (MQ=255)

                                                                                                                                         gggCCAGTTCCGCCGGGAACTGGCCACGGTCGAACAGCTCGGGGTCCACCTGGGGCATCGCGTCACGTTCCTGTCGTGGGGTGGCCTGTCGGGGTGCGGTCAGGCCGAAGTGCGCGGGATGGTGTCGTCCTTGCGCAGGGTGAAGAtctc            >  2:296858/1‑150 (MQ=255)

                                                                                                                                          ggCCAGTTCCGCCTGGAACTGGCCACGGTCGAACAGCTCGGGGTCCACCTGGGGCATCGCGTCACGTTCCTGTCGTGGGGTGGCCTGTCGGGGTGCGGTCAGGCCGAAGTGCGCGGGATGGTGTCGTCCTTGCGCAGGGTGAAGATCTCg           <  2:3882767/150‑1 (MQ=255)

                                                                                                                                          ggCCAGTTCCGCCCGGAACTGGCCACGGTCGAACAGCTCGGGGTCCACCTGGGGCATCGCGTCACGTTCCTGTCGTGGGGTGGCCTGTCGGGGTGCGGTCAGGCCGAAGTGCGCGGGATGGTGTCGTCCTTGCGCAGGGTGAAGATCTCg           <  1:296858/150‑1 (MQ=255)

                                                                                                                                           gCCAGTTCCAGGCGGAACTGGCCACGGCCGAACAGCTCGGGGTCCACCTGGGGCATCGCGTCACGTTCCTGTCGTGGGGTGGCCTGTCGGGGTGCGGTCAGGCCGAAGTGCGCGGGATGGTGTCGTCCTTGCGCAGGGTGAAGATCTCGt          >  1:3282899/1‑150 (MQ=255)

                                                                                                                                             cAGTTCCGCCTGGAACTGGCCACGGTCGAACAGCTCGGGGTCCACCTGGGGCATCGCGTCACGTTCCTGTCGTGGGGTGGCCTGTCGGGGTGCGGTCAGGCCGAAGTGCGCGGGATGGTGTCGTCCTTGCGCAGGGTGAAGATCTCGTAg        >  2:2497591/1‑150 (MQ=255)

                                                                                                                                             cAGTTCCGCCTGGAACTGGCCACGGTCGAACAGCTCGGGGTCCACCTGGGGCATCGCGTCACGTTCCTGTCGTGGGGTGGCCTGTCGGGGTGCGGTCAGGCCGAAGTGCGCGGGATGGTGTCGTCCTTGCGCAGGGTGAAGATCTCGTAg        >  2:1086692/1‑150 (MQ=255)

                                                                                                                                              aGTTCCGCCTGGAACTGGCCACGGTCGAACAGCTCGGGGTCCACCTGGGGCATCGCGTCACGTTCCTGTCGTGGGGTGGCCTGTCGGGGTGCGGTCAGGCCGAAGTGCGCGGGATGGTGTCGTCCTTGCGCAGGGTGAAGATCTCGTAGc       <  1:5081470/150‑1 (MQ=255)

                                                                                                                                                ttCCGCCTGGAACTGGCCACGGTCGAACAGCTCGGGGTCCACCTGGGGCATCGCGTCACGTTCCTGTCGTGGGGTGGCCTGTCGGGGTGCGGTCAGGCCGAAGTGCGCGGGATGGTGTCGTCCTTGCGCAGGGTGAAGATCTCGTAGCCa     <  1:2905235/150‑1 (MQ=255)

TTGCAGGATATTGTCGACGAGCCAGGCGCGGGCCTCGATCAGCGGTCGGATGTCGCTGCCGGCGCGAAAACGTTTGTCGAGCACCTCGCCGGCCAGGCGGATGGCTTTCTTGAAGGCGGCGATGGGGCTCGCCTTGAGGGCCAGTTCCGCCTGGAACTGGCCACGGTCGAACAGCTCGGGGTCCACCTGGGGCATCGCGTCACGTTCCTGTCGTGGGGTGGCCTGTCGGGGTGCGGTCAGGCCGAAGTGCGCGGGATGGTGTCGTCCTTGCGCAGGGTGAAGATCTCGTAGCCAGTG  >  NC_002947/1783924‑1784220

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`