BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	1,831,822	T→C	9.8%	L220P (CTG→CCG)	yfiP →	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	1,831,822	0	T	C	9.8%	445.3 / 27.1	153	L220P (CTG→CCG)	yfiP	hypothetical protein
Reads supporting (aligned to +/- strand): ref base T (58/80); new base C (7/8); total (65/88)
Fisher's exact test for biased strand distribution p-value = 7.87e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.65e-01

Only 100 of 153 total aligned reads displayed.

CTGCGGCGCTCCAAGCGCGATGATCATTTCTGTACGGCAGAAGTTGCGGCGATGTGCCTGGACCTTGCTGGCGATGGCCAGGCTTCGCAGGCGCTGGATGCCTATCTGGATGTGTTCAGCCTGCATTACCTGAGCGGCAAGCGGCGGCTGCCGCTTGACGAGCAGGACGACACCCACCAGCGTTTGCATACCTTCCTATAGTCCAAGGGGCACAACCCCTGCTTTGGTTCATAATGACGGAGCTGGCAGCCAGGGTGGGCGAGACGGCAGGGCGATTGGCTTGATCTCCCTTGACCG  >  NC_002947/1831674‑1831970

cTGCGGCGCTCCAAGCGCGATGATCATTTCTGTACGGCAGAAGTTGCGGCGATGTGCCTGGACCTTGCTGGCGATGGCCAGGCTTCGCAGGCGCTGGATGCCTATCTGGATGTGTTCAGCCTGCATTACCTGAGCGGCAAGCGGCGGCTg                                                                                                                                                     >  1:1867102/1‑150 (MQ=255)

    ggcgCTCCAAGCGCGATGATCATTTCTGTACGGCAGAAGTTGCGGCGATGTGCCTGGACCTTGCTGGCGATGGCCAGGCTTCGCAGGCGCTGGATGCCTATCTGGATGTGTTCAGCCTGCATTACCTGAGCGGCAAGCGGCGGCTGCCGc                                                                                                                                                 <  1:2005243/150‑1 (MQ=255)

      cgcTCCAAGCGCGATGATCATTTCTGTACGGCAGAAGTTGCGGCGATGTGCCTGGACCTTGCTGGCGATGGCCAGGCTTCGCAGGCGCTGGATGCCTATCTGGATGTGTTCAGCCTGCATTACCTGAGCGGCAAGCGGCGGCTGCCGCtt                                                                                                                                               >  1:4948159/1‑150 (MQ=255)

       gcTCCAAGCGCGATGATCATTTCTGTACGGCAGAAGTTGCGGCGATGTGCCTGGACCTTGCTGGCGATGGCCAGGCTTCGCAGGCGCTGGATGCCTATCTGGATGTGTTCAGCCTGCATTACCTGAGCGGCAGCCGCCGCTTGCCGCtca                                                                                                                                              <  2:4367333/150‑3 (MQ=255)

         tCCAAGCGCGATGATCATTTCTGTACGGCAGAAGTTGCGGCGATATGCCTGGACCTTGCTGGCGATGGCCAGGCTTCGCAGGCGCTGGATGCCTATCTGGATGTGTTCAGCCTGCATTACCTGAGCGGCAAGCGGCGGCTGCCGCTTGAc                                                                                                                                            >  1:5350656/1‑150 (MQ=255)

           cAAGCGCGATGATCATTTCTGTACGGCAGAAGTTGCGGCGATGTGCCTGGACCTTGCTGGCGATGGCCAGGCTTCGCAGGCGCTGGATGCCTATCTGGATGTGTTCAGCCTGCATTACCTGAGCGGCAAGCGGCGGCTGCCGCTTGACGa                                                                                                                                          <  1:2261349/150‑1 (MQ=255)

            aaGCGCGATGATCATTTCTGTACGGCAGAAGTTGCGGCGATGTGCCTGGACCTTGCTGGCGATGGCCAGGCTTCGCAGGCGCTGGATGCCTATCTGGATGTGTTCAGCCTGCATTACCTGAGCGGCAAGCGGCGGCTGCCGCTTGACGAg                                                                                                                                         >  2:2339358/1‑150 (MQ=255)

             aGCGCGATGATCATTTCTGTACGGCAGAAGTTGCGGCGATGTGCCTGGACCTTGCTGGCGATGGCCAGGCTTCGCAGGCGCTGGATGCCTATCTGGATGTGTTCAGCCTGCATTACCTGAGCGGCAAGCGGCGGCTGCCGCTTGACGAGc                                                                                                                                        <  2:754358/150‑1 (MQ=255)

             aGCGCGATGATCATTTCTGTACGGCAGAAGTTGCGGCGATATGCCTGGACCTTGCTGGCGATGGCCAGGCTTCGCAGGCGCTGGATGCCTATCTGGATGTGTTCAGCCTGCATTACCTGAGCGGCAAGCGGCGGCTGCCGCTTGACGAGc                                                                                                                                        <  2:5350656/150‑1 (MQ=255)

              gcgcGATGATCATTTCTGTACGGCAGAAGTTGCGGCGATGTGCCTGGACCTTGCTGGCGATGGCCAGGCTTCGCAGGCGCTGGATGCCTATCTGGATGTGTTCAGCCTGCATTACCTGAGCGTCAAGCGGCAGCCGCCGCTTGCCgctca                                                                                                                                       <  2:235581/150‑5 (MQ=255)

              gcgcGATGATCATTTCTGTACGGCAGAAGTTGCGGCGATGTGCCTGGACCTTGCTGGCGATGGCCAGGCTTCGCAGGCGCTGGATGCCTATCTGGATGTGTTCAGCCTGCATTACCTGAGCGGCAAGCGGCGGCTGCCGCTTGACGAGCa                                                                                                                                       <  1:1418028/150‑1 (MQ=255)

               cgcgATGATCATTTCTGTACGGCAGAAGTTGCGGCGATGTGCCTGGACCTTGCTGGCGATGGCCAGGCTTCGCAGGCGCTGGATGCCTATCTGGATGTGTTCAGCCTGCATTACCTGCTCGTCAAGCGGCAGCCGCCGCTTGCCgctcag                                                                                                                                      <  1:4942873/150‑6 (MQ=38)

                gcgATGATCATTTCTGTACGGCAGAAGTTGCGGCGATGTGCCTGGACCTTGCTGGCGATGGCCAGGCTTCGCAGGCGCTGGATGCCTATCTGGATGTGTTCAGCCTGCATTACCTGAGCGGCAAGCGGCGGCTGCCGCTTGACGAGCAgg                                                                                                                                     <  1:5154733/150‑1 (MQ=255)

                  gatgatCATTTCTGTACGGCAGAAGTTGCGGCGATGTGCCTGGACCTTGCTGGCGATGGCCAGGCTTCGCAGGCGCTGGATGCCTATCTGGATGTGTTCAGCCTGCATTACCTGAGCGGCAAGCGGCGGCTGCCGCTTGACGAGCAGgac                                                                                                                                   >  2:1837684/1‑150 (MQ=255)

                    tgatCATTTCTGTACGGCAGAAGTTGCGGCGATGTGCCTGGACCTTGCTGGCGATGGCCAGGCTTCGCAGGCGCTGGATGCCTATCTGGATGTGTTCAGCCTGCATTACCTGAGCGGCAAGCGGCGGCTGCCGCTTGACGAGCAGgacga                                                                                                                                 <  2:1060527/150‑1 (MQ=255)

                     gatCATTTCTGTACGGCAGAAGTTGCGGCGATGTGCCTGGACCTTGCTGGCGATGGCCAGGCTTCGCAGGCGCTGGATGCCTATCTGGATGTGTTCAGCCTGCATTACCTGAGCGGCAAGCGGCGGCTGCCGCTTGACGAGCAGgacgac                                                                                                                                >  1:1931681/1‑150 (MQ=255)

                     gatCATTTCTGTACGGCAGAAGTTGCGGCGATGTGCCTGGACCGTGCTGGCGATGGCCAGGCTTCGCAGGCGCTGGATGCCTATCTGGATGTGTTCAGCCTGCATTACCTGAGCGGCAAGCGGCGGCTGCCGCTTGACGAGCAGgacgac                                                                                                                                <  2:5391706/150‑1 (MQ=255)

                      atCATTTCTGTACGGCAGAAGTTGCGGCGATGTGCCTGGACCTTGCTGGCGATGGCCAGGCTTCGCAGGCGCTGGATGCCTATCTGGATGTGTTCAGCCTGCATTACCTGAGCGGCAAGCGGCAGCCGCCGCTTgc                                                                                                                                             >  2:49151/1‑135 (MQ=255)

                        cATTTCTGTACGGCAGAATTTGCGGCGATGTGCCTGGACCTTGCTGGCGATGGCCAGGCTTCGCAGGCGCTGGATGCCTATCTGGATGTGTTCAGCCTGCATTACCTGAGCGGCAAGCGGCGGCTGCCGCTTGACGAGCAGGACGAcacc                                                                                                                             <  1:4293925/150‑1 (MQ=255)

                        cATTTCTGTACGGCAGAAGTTGCGGCGATGTGCCTGGACCTTGCTGGCGATGGCCAGGCTTCGCAGGCGCTGGATGCCTATCTGGATGTGTTCAGCCTGCATTACCTGAGCGGCAAGCGGCGGCTGCCGCTTGACGAGCAGGACGAcacc                                                                                                                             <  1:4293940/150‑1 (MQ=255)

                         aTTTCTGTACGGCAGAAGTTGCGGCGATGTGCCTGGACCTTGCTGGCGATGGCCAGGCTTCGCGGGCGCTGGATGCCTATCTGGATGTGTTCAGCCTGCATTACCTGAGCGGCAAGCGGCGGCTGCCGCTTGACGAGCAGGACGAcaccc                                                                                                                            <  1:3165017/150‑1 (MQ=255)

                          tttCTGTACGGCAGAAGTTGCGGCGATGTGCCTGGACCTTGCTGGCGATGGCCAGGCTTCGCAGGCGCTGGATGCCTATCTGGATGTGTTCAGCCTGCATTACCTGAGCGGCAAGCGGCGGCTGCCGCTTGACGAGCAGGACGAcaccca                                                                                                                           >  2:1291515/1‑150 (MQ=255)

                               gTACGGCAGAAGTTGCGGCGATGTGCCTGGACCTTGCTGGCGATGGCCAGGCTTCGCAGGCGCTGGATGCCTATCTGGATGTGTTCAGCCTGCATTACCTGAGCGGCAAGCGGCGGCTGCCGCTTGACGAGCAGGACGACACCCACCAGc                                                                                                                      <  1:4981066/150‑1 (MQ=255)

                               gTACGGCAGAAGTTGCGGCGATGTGCCTGGACCTTGCTGGCGATGGCCAGGCTTCGCAGGCGCTGGATGCCTATCTGGATGTGTTCAGCCTGCATTACCTGAGCGGCAAGCGGCGGCTGCCGCTTGACGAGCAGGACGACACCCACCAGc                                                                                                                      >  2:5108558/1‑150 (MQ=255)

                                tACGGCAGAAGTTGCGGCGATGTGCCTGGACCTTGCTGGCGATGGCCAGGCTTCGCAGGCGCTGGATGCCTATCTGGATGTGTTCAGCCTGCATTACCTGAGCGGCAAGCGGCGGCTGCCGCTTGACGAGCAGGACGACACCCACCAGCg                                                                                                                     >  1:1990827/1‑150 (MQ=255)

                                  cGGCAGAAGTTGCGGCGATGTGCCTGGACCTTGCTGGCGATGGCCAGGCTTCGCAGGCGCTGGATGCCTATCTGGATGTGTTCAGCCTGCATTACCTGAGCGGCAAGCGGCGGCTGCCGCTTGACGAGCAGGACGACACCCACCAGCGtt                                                                                                                   <  1:986530/150‑1 (MQ=255)

                                   ggCAGAAGTTGCGGCGATGTGCCTGGACCTTGCTGGCGATGGCCAGGCTTCGCAGGCGCTGGATGCCTATCTGGATGTGTTCAGCCTGCATTACCTGAGCGGCAAGCGGCGGCTGCCGCTTGACGAGCAGGACGACACCCACCAGCGttt                                                                                                                  <  1:875391/150‑1 (MQ=255)

                                    gCAGAAGTTGCGGCGATGTGCCTGGACCTTGCTGGCGATGGCCAGGCTTCGCAGGCGCTGGATGCCTATCTGGATGTGTTCAGCCTGCATTACCTGAGCGGCAAGCGGCGGCTGCCGCTTGACGAGCAGGACGACACCCACCAGCGTTTg                                                                                                                 <  1:3949255/150‑1 (MQ=255)

                                    gCAGAAGTTGCGGCGATGTGCCTGGACCTTGCTGGCGATGGCCAGGCTTCGCAGGCGCTGGATGCCTATCTGGATGTGTTCAGCCTGCATTACCTGAGCGGCAAGCGGCGGCTGCCGCTTGACGAGCAGGACGACACCCACCAGCGTTTg                                                                                                                 <  1:2292022/150‑1 (MQ=255)

                                     cAGAAGTTGCGGCGATGTGCCTGGACCTTGCTGGCGATGGCCAGGCTTCGCAGGCGCTGGATGCCTATCTGGATGTGTTCAGCCTGCATTACCTGAGCGGCAAGCGGCGGCTGCCGCTTGACGAGCAGGACGACACCCACCAGCGTTTGc                                                                                                                >  2:3839753/1‑150 (MQ=255)

                                       gAAGTTGCGGCGATGTGCCTGGACCTTGCTGGCGATGGCCAGGCTTCGCAGGCGCTGGATGCCTATCTGGATGTGTTCAGCCTGCATTACCTGAGCGGCAAGCGGCGGCTGCCGCTTGACGAGCAGGACGACACCCACCAGCGTTTGCAt                                                                                                              >  1:4700639/1‑150 (MQ=255)

                                                   aTGTGCCTGGACCTTGCTGGCGATGGCCAGGCTTCGCAGGCGCTGGATGCCTATCTGGATGTGTTCAGCCTGCATTACCTGAGCGGCAAGCGGCGGCTGCCGCTTGACGAGCAGGACGACACCCACCAGCGTTTGCATACCTTCCTATAg                                                                                                  <  1:5133401/150‑1 (MQ=255)

                                                       gCCTGGACCTTGCTGGCGATGGCCAGGCTTCGCAGGCGCTGGATGCCTATCTGGATGTGTTCAGCCTGCATTACCTGAGCGGCAAGCGGCGGCTGCCGCTTGACGAGCAGGACGACACCCACCAGCGTTTGCATACCTTCCTATAGTCCa                                                                                              >  2:1082521/1‑150 (MQ=255)

                                                        ccTGGACCTTGCTGGCGATGGCCAGGCTTCGCAGGCGCTGGATGCCTATCTGGATGTGTTCAGCCTGCATTACCTGAGCGGCAAGCGGCGGCTGCCGCTTGACGAGCAGGACGACACCCACCAGCGTTTGCATACCTTCCTATAGTCCaa                                                                                             <  2:2080813/150‑1 (MQ=255)

                                                         cTGGACCTTGCTGGCGATGGCCAGGCTTCGCAGGCGCTGGATGCCTATCTGGATGTGTTCAGCCTGCATTACCTGAGCGGCAAGCGGCGGCTGCCGCTTGACGAGCAGGACGACACCCACCAGCGTTTGCATACCTTCCTATAGTCCAAg                                                                                            <  1:5108558/150‑1 (MQ=255)

                                                         cTGGACCTTGCTGGCGATGGCCAGGCTTCGCAGGCGCTGGATGCCTATCTGGATGTGTTCAGCCTGCATTACCTGAGCGGCAAGCGGCGGCTGCCGCTTGACGAGCAGGACGACACCCACCAGCGTTTGCATACCTTCCTATAGTCCAAg                                                                                            >  2:1952944/1‑150 (MQ=255)

                                                          tGGACCTTGCTGGCGATGGCCAGGCTTCGCAGGCGCTGGATGCCTATCTGGATGTGTTCAGCCTGCATTACCTGAGCGGCAAGCGGCGGCTGCCGCTTGACGAGCAGGACGACACCCACCAGCGTTTGCATACCTTCCTATAGTCCAAgg                                                                                           <  2:2902423/150‑1 (MQ=255)

                                                           ggACCTTGCTGGCGATGGCCAGGCTTCGCAGGCGCTGGATGCCTATCTGGATGTGTTCAGCCTGCATTACCTGAGCGGCAAGCGGCGGCTGCCGCTTGACGAGCAGGACGACACCCACCAGCGTTTGCATACCTTCCTATAGTCCAAggg                                                                                          <  1:1454608/150‑1 (MQ=255)

                                                           ggACCTTGCTGGCGATGGCCAGGCTTCGCAGGCGCTGGATGCCTATCTGGATGTGTTCAGCCTGCATTACCTGAGCGGCAAGCGGCGGCTGCCGCTTGACGAGCAGGACGACACCCACCAGCGTTTGCATACCTTCCTATAGTCCAAggg                                                                                          <  1:1454463/150‑1 (MQ=255)

                                                            gACCTTGCTGGCGATGGCCAGGCTTCGCAGGCGCTGGATGCCTATCTGGATGTGTTCAGCCTGCATTACCTGAGCGGCAAGCGGCGGCTGCCGCTTGACGAGCAGGACGACACCCACCAGCGTTTGCATACCTTCCTATAGTCCAAgggg                                                                                         >  2:1639317/1‑150 (MQ=255)

                                                              ccTTGCTGGCGATGGCCAGGCTTCGCAGGCGCTGGATGCCTATCTGGATGTGTTCAGCCTGCATTACCTGAGCGGCAAGCGGCGGCTGCCGCTTGACGAGCAGGACGACACCCACCAGCGTTTGCATACCTTCCTATAGTCCAAGGGGca                                                                                       >  1:3005691/1‑150 (MQ=255)

                                                                            gCCAGGCTTCGCAGGCGCTGGATGCCTATCTGGATGTGTTCAGCCTGCATTACCTGAGCGGCAAGCGGCGGCTGCCGCTTGACGAGCAGGACGACACCCACCAGCGTTTGCATACCTTCCTATAGTCCAAGGGGCACAACCCCTGCTTTg                                                                         <  1:4492988/150‑1 (MQ=255)

                                                                            gCCAGGCTTCGCAGGCGCTGGATGCCTATCTGGATGTGTTCAGCCTGCATTACCTGAGCGGCAAGCGGCGGCTGCCGCTTGACGAGCAGGACGACACCCACCAGCGTTTGCATACCTTCCTATAGTCCAAGGGGCACAACCCCTGCTTTg                                                                         <  1:3370321/150‑1 (MQ=255)

                                                                            gCCAGGCTTCGCAGGCGCTGGATGCCTATCTGGATGTGTTCAGCCTGCATTACCTGAGCGGCAAGCGGCGGCTGCCGCTTGACGAGCAGGACGACACCCACCAGCGTTTGCATACCTTCCTATAGTCCAAGGGGCACAACCCCTGCTTTg                                                                         <  2:1563613/150‑1 (MQ=255)

                                                                             ccAGGCTTCGCAGGCGCTGGATGCCTATCTGGATGTGTTCAGCCTGCATTACCTGAGCGGCAAGCGGCGGCTGCCGCTTGACGAGCAGGACGACACCCACCAGCGttt                                                                                                                  >  2:3965490/1‑108 (MQ=255)

                                                                             ccAGGCTTCGCAGGCGCTGGATGCCTATCTGGATGTGTTCAGCCTGCATTACCTGAGCGGCAAGCGGCGGCTGCCGCTTGACGAGCAGGACGACACCCACCAGCGTTTGCATACCTTCCTATAGTCCAAGGGGCACAACCCCTGCTTTgg                                                                        >  2:2667482/1‑150 (MQ=255)

                                                                             ccAGGCTTCGCAGGCGCTGGATGCCTATCTGGATGTGTTCAGCCTGCATTACCTGAGCGGCAAGCGGCGGCTGCCGCTTGACGAGCAGGACGACACCCACCAGCGTTTGCATACCTTCCTATAGTCCAAGGGGCACAACCCCTGCTTTgg                                                                        >  1:3374859/1‑150 (MQ=255)

                                                                              cAGGCTTCGCAGGCGCTGGATGCCTATCTGGATGTGTTCAGCCTGCATTACCTGAGCGGCAAGCGGCGGCTGCCGCTTGACGAGCAGGACGACACCCACCAGCGTTTGCATACCTTCCTATAGTCCAAgg                                                                                           >  2:111511/1‑130 (MQ=255)

                                                                              cAGGCTTCGCAGGCGCTGGATGCCTATCTGGATGTGTTCAGCCTGCATTACCTGAGCGGCAAGCGGCGGCTGCCGCTTGACGAGCAGGACGACACCCACCAGCGTTTGCATACCTTCCTATAGTCCAAgg                                                                                           <  1:111511/130‑1 (MQ=255)

                                                                              cAGGCTTCGCAGGCGCTGGATGCCTATCTGGATGTGTTCAGCCTGCATTACCTGAGCGGCAAGCGGCGGCTGCCGCTTGACGAGCAGGACGACACCCACCAGCGTTTGCATACCTTCCTATAGTCCAAGGGGCACAACCCCTGCTTTGGt                                                                       <  2:3607076/150‑1 (MQ=255)

                                                                               aGGCTTCGCAGGCGCTGGATGCCTATCTGGATGTGTTCAGCCTGCATTACCTGAGCGGCAAGCGGCGGCTGCCGCTTGACGAGCAGGACGACACCCACCAGCGTTTGCATACCTTCCTATAGTCCAAGGGGCACAACCCCTGCTTTGGtt                                                                      <  2:1829552/150‑1 (MQ=255)

                                                                                   ttCGCAGGCGCTGGATGCCTATCTGGATGTGTTCAGCCTGCATTACCTGAGCGGCAAGCGGCGGCTGCCGCTTGACGAGCAGGACGACACCCACCAGCGTTTGCATACCTTCCTATAGTCCAAGGGGCACAACCCCTGCTTTGGTTCATa                                                                  >  2:3430094/1‑150 (MQ=255)

                                                                                     cGCAGGCGCTGGATGCCTATCTGGATGTGTTCAGCCTGCATTACCTGAGCGGCAAGCGGCGGCTGCCGCTTGACGAGCAGga                                                                                                                                    >  1:2469079/1‑82 (MQ=255)

                                                                                       cAGGCGCTGGATGCCTATCTGGATGTGTTCAGCCTGCATTACCTGAGCGGCAAGCGGCGGCTGCCGCTTGACGAGCAGGACGACACCCACCAGCGTTTGCATACCTTCCTATAGTCCAAGGGGCACAACCCCTGCTTTGGTTCATAATGa                                                              <  1:1291515/150‑1 (MQ=255)

                                                                                        aGGCGCTGGATGCCTATCTGGATGTGTTCAGCCTGCATTACCTGAGCGGCAAGCGGCGGCTGCCGCTTGACGAGCAGGACGACACCCACCAGCGTTTGCATACCTTCCTATAGTCCAAGGGGCACAACCCCTGCTTTGGTTCATAATGAc                                                             <  1:4490979/150‑1 (MQ=255)

                                                                                         ggCGCTGGATGCCTATCTGGATGTGTTCAGCCTGCATTACCTGAGCGGCAAGCGGCGGCTGCCGCTTGACGAGCAGGACGACACCCACCAGCGTTTGCATACCTTCCTATAGTCCAAGGGGCACAACCCCTGCTTTGGTTCATAATGACg                                                            <  1:3535856/150‑1 (MQ=255)

                                                                                         ggCGCTGGATGCCTATCTGGATGTGTTCAGCCTGCATTACCTGAGCGGCAAGCGGCGGCTGCCGCTTGACGAGCAGGACGACACCCACCAGCGTTTGCATACCTTCCTATAGTCCAAGGGGCACAACCCCTGCTTTGGTTCATAATGACg                                                            <  1:5109968/150‑1 (MQ=255)

                                                                                           cgcTGGATGCCTATCTGGATGTGTTCAGCCTGCATTACCTGAGCGGCAAGCGGCGGCTGCCGCTTGACGAGCAGGACGACACCCACCAGCGTTTGCATACCTTCCTATAGTCCAAGGGGCACAACCCCTGCTTTGGTTCATAATGACGGa                                                          >  2:2887032/1‑150 (MQ=255)

                                                                                           cgcTGGATGCCTATCTGGATGTGTTCAGCCTGCATTACCTGAGCGGCAAGCGGCGGCTGCCGCTTGACGAGCAGGACGACACCCACCAGCGTTTGCATACCTTCCTATAGTCCAAGGGGCACAACCCCTGCTTTGGTTCATAATGACGGa                                                          <  2:2047681/150‑1 (MQ=255)

                                                                                              tGGATGCCTATCTGGATGTGTTCAGCCTGCATTACCTGAGCGGCAAGCGGCGGCTGCCGCTTGACGAGCAGGACGACACCCACCAGCGTTTGCATACCTTCCTATAGTCCAAGGGGCACAACCCCTGCTTTGGTTCATAATGACGGAGCt                                                       >  2:91686/1‑150 (MQ=255)

                                                                                               ggATGCCTATCTGGATGTGTTCAGCCTGCATTACCTGAGCGGCAAGCGGCGGCTGCCGCTTGACGAGCAGGACGACACCCACCAGCGTTTGCATACCTTCCTATAGTCCAAGGGGCACAACCCCTGCTTTGGTTCATAATGACGGAGCTg                                                      <  1:1774090/150‑1 (MQ=255)

                                                                                                gATGCCTATCTGGATGTGTTCAGCCTGCATTACCTGAGCGGCAAGCGGCGGCTGCCGCTTGACGAGCAGGACGACACCCACCAGCGTTTGCATACCTTCCTATAGTCCAAGGGGCACAACCCCTGCTTTGGTTCATAATGACGGAGCTgg                                                     <  2:1703167/150‑1 (MQ=255)

                                                                                                gATGCCTATCTGGATGTGTTCAGCCTGCATTACCTGAGCGGCAAGCGGCGGCTGCCGCTTGACGAGCAGGACGACACCCACCAGCGTTTGCATACCTTCCTATAGTCCAAGGGGCACAACCCCTGCTTTGGTTCATAATGACGGAGCTgg                                                     <  2:3761624/150‑1 (MQ=255)

                                                                                                 aTGCCTATCTGGATGTGTTCAGCCTGCATTACCTGAGCGGCAAGCGGCGGCTGCCGCTTGACGAGCAGGACGACACCCACCAGCGTTTGCATACCTTCCTATAGTCCAAGGGGCACAACCCCTGCTTTGGTTCATAATGACGGAGCTGGc                                                    <  1:5280035/150‑1 (MQ=255)

                                                                                                  tGCCTATCTGGATGTGTTCAGCCTGCATTACCTGAGCGGCAAGCGGCGGCTGCCGCTTGACGAGCAGGACGACACCCACCAGCGTTTGCATACCTTCCTATAGTCCAAGGGGCACAACCCCTGCTTTGGTTCATAATGACGGAGCTGGCa                                                   <  2:4212415/150‑1 (MQ=255)

                                                                                                      tATCTGGATGTGTTCAGCCTGCATTACCTGAGCGGCAAGCGGCGGCTGCCGCTTGACGAGCAGGACGACACCCACCAGCGTTTGCATACCTTCCTATAGTCCAAGGGGCACAACCCCTGCttt                                                                          <  2:2285774/123‑1 (MQ=255)

                                                                                                        tCTGGATGTGTTCAGCCTGCATTACCCGAGCGGCAAGCGGCGGCTGCCGCTTGACGAGCAGGACGACACCCACCAGCGTTTGCATACCTTCCTATAGTCCAAGGGGCACAACCCCTGCTTTGGTTCATAATGACGGAGCTGGCAGCCAgg                                             <  2:4436677/150‑1 (MQ=255)

                                                                                                            gATGTGTTCAGCCTGCATTACCTGAGCGGCAAGCGGCGGCTGCCGCTTGACGAGCAGGACGACACCCACCAGCGTTTGCATACCTTCCTATAGTCCAAGGGGCACAACCCCTGCTTTGGTTCATAATGACGGAGCTGGCAGCCAGGGTgg                                         >  1:280367/1‑150 (MQ=255)

                                                                                                              tgtgTTCAGCCTGCATTACCTGAGCGGCAAGCGGCGGCTGCCGCTTGACGAGCAGGACGACACCCACCAGCGTTTGCATACCTTCCTATAGTCCAAGGGGCACAACCCCTGCTTTGGTTCATAATGACGGAGCTGGCAGCCAGGGTGGGc                                       <  2:4948159/150‑1 (MQ=255)

                                                                                                                 gtTCAGCCTGCATTACCTGAGCGGCAAGCGGCGGCTGCCGCTTGACGAGCAGGACGACACCCACCAGCGTTTGCATACCTTCCTATAGTCCAAGGGGCACAACCCCTGCTTTGGTTCATAATGACGGAGCTGGCAGCCAGGGTGGGCgat                                    >  1:3558843/1‑149 (MQ=255)

                                                                                                                  ttCAGCCTGCATTACCTGAGCGGCAAGCGGCGGCTGCCGCTTGACGAGCAGGACGACACCCACCAGCGTTTGCATACCTTCCTATAGTCCAAGGGGCACAACCCCTGCTTTGGTTCATAATGACGGAGCTGGCAGCCAGGGTGGGCgaga                                   >  2:2271506/1‑150 (MQ=255)

                                                                                                                     aGCCTGCATTACCTGAGCGGCAAGCGGCGGCTGCCGCTTGACGAGCAGGACGACACCCACCAGCGTTTGCATACCTTCCTATAGTCCAAGGGGCACAACCCCTGCTTTGGTTCATAATGACGGAGCTGGCAGCCAGGGTGGGCGAGACgg                                >  2:1699406/1‑150 (MQ=255)

                                                                                                                       ccTGCATTACCTGAGCGGCAAGCGGCGGCTGCCGCTTGACGAGCAGGACGACACCCACCAGCGTTTGCATACCTTCCTATAGTCCAAGGGGCACAACCCCTGCTTTGGTTCATAATGACGGAGCTGGCAGCCAGGGTGGGCGAGACGGCa                              >  1:922984/1‑150 (MQ=255)

                                                                                                                       ccTGCATTACCTGAGCGGCAAGCGGCGGCTGCCGCTTGACGAGCAGGACGACACCCACCAGCGTTTGCATACCTTCCTATAGTCCAAGGGGCACAACCCCTGCTTTGGTTCATAATGACGGAGCTGGCAGCCAGGGTGGGCGAGACGGCa                              <  1:4810170/150‑1 (MQ=255)

                                                                                                                       ccTGCATTACCTGAGCGGCAAGCGGCGGCTGCCGCTTGACGAGCAGGACGACACCCACCAGCGTTTGCATACCTTCCTATAGTCCAAGGGGCACAACCCCTGCTTTGGTTCATAATGACGGAGCTGGCAGCCAGGGTGGGCGAGACGGCa                              <  1:4810256/150‑1 (MQ=255)

                                                                                                                       ccTGCATTACCTGAGCGGCAAGCGGCGGCTGCCGCTTGACGAGCAGGACGACACCCACCAGCGGTTGCATACCTTCCTATAGTCCAAGGGGCACAACCCCTGCTTTGGTTCATAATGACGGAGCTGGCAGCCAGGGTGGGCGAGACGGCa                              <  1:3939186/150‑1 (MQ=255)

                                                                                                                        cTGCATTACCTGAGCGGCAAGCGGCGGCTGCCGCTTGACGAGCAGGACGACACCCACCAGCGTTTGCATACCTTCCTATAGTCCAAGGGGCACAACCCCTGCTTTGGTTCATAATGACGGAGCTGGCAGCCAGGGTGGGCGAGACGGCAg                             <  2:3719780/150‑1 (MQ=255)

                                                                                                                            aTTACCTGAGCGGCAAGCGGCGGCTGCCGCTTGACGAGCAGGACGACACCCACCAGCGTTTGCATACCTTCCTATAGTCCAAGGGGCACAACCCCTGCTTTGGTTCATAATGACGGAGCTGGCAGCCAGGGTGGGCGAGACGGCAGGGCg                         >  1:2535388/1‑150 (MQ=255)

                                                                                                                                ccTGAGCTGCAAGCGGCGGCTGCCGCTTGACGAGCAGGACGACACCCACCAGCGTTTGCATACCTTCCTATAGTCCAAGGGGCACAACCCCTGCTTTGGTTCATAATGACGGAGCTGGCAGCCAGGGTGGGCGAGACGGCAGGGCGATTg                     >  2:5055545/1‑150 (MQ=255)

                                                                                                                                  tgctcgtcAAGCGGCAGCCGCCGCTTGACGAGCAGGACGACACCCACCAGCGGTTGCATACCTTCCTATAGTCCAAGGGGCACAACCCCTGCTTTGGTTCATAATGACGGAGCTGGCAGCCAGGGTGGGCGAGACGGCAGGGCGATTGGc                   >  2:2001626/8‑150 (MQ=255)

                                                                                                                                  tGAGCGGCAAGCGGCGGCTGCCGCTTGACGAGCAGGACGACACCCACCAGCGTTTGCATACCTTCCTATAGTCCAAGGGGCACAACCCCTGCTTTGGTTCATAATGACGGAGCTGGCAGCCAGGGTGGGCGAGACGGCAGGGCGATTGGc                   >  2:2537192/1‑150 (MQ=255)

                                                                                                                                  tGAGCGGCAAGCGGCGGCTGCCGCTTGACGAGCAGGACGACACCCACCAGCGTTTGCATACCTTCCTATAGTCCAAGGGGCACAACCCCTGCTTTGGTTCATAATGACGGAGCTGGCAGCCAGGGTGGGCGAGACGGCAGGGCGATTGGc                   <  2:730622/150‑1 (MQ=255)

                                                                                                                                  tGAGCGGCAAGCGGCGGCTGCCGCTTGACGAGCAGGACGACACCCACCAGCGTTTGCATACCTTCCTATAGTCCAAGGGGCACAACCCCTGCTTTGGTTCATAATGACGGAGCTGGCAGCCAGGGTGGGCGAGACGGCAGGGCGATTGGc                   >  2:5264533/1‑150 (MQ=255)

                                                                                                                                   aaGCGGCAGCCGCCGCTTGCCGCTTGACGAGCAGGACGACACCCACCAGCGTTTGCATACCTTCCTATAGTCCAAGGGGCACAACCCCTGCtt                                                                           >  1:1552980/2‑93 (MQ=38)

                                                                                                                                   aaGCGGCAGCCGCCGCTTGCCGCTTGACGAGCAGGACGACACCCACCAGCGTTTGCATACCTTCCTATAGTCCAAGGGGCACAACCCCTGCtt                                                                           <  2:1552980/92‑1 (MQ=38)

                                                                                                                                    aGCGGCAAGCGGCGGCTGCCGCTTGACGAGCAGGACGACACCCACCAGCGTTTGCATACCTTCCTATAGTCCAAGGGGCACAACCCCTGCTTTGGTTCATAATGACGGAGCTGGCAGCCAGGGTGGGCGAGACGGCAGGGCGATTGGCtt                 <  1:2246503/150‑1 (MQ=255)

                                                                                                                                     tcgtcAAGCGGCAGCCGCCGCTTGCCGAGCAGGACGACACCCACCAGCGTTTGCATACCTTCCTATAGTCCAAGGGGCACAACCCCTGCTTTGGTTCATAATGACGGAGCTGGCAGCCAGGGTGGGCGAGACGGCAGGGCGATTGGCTTg                >  2:1300630/5‑150 (MQ=255)

                                                                                                                                     gCGGCAAGCGGCGGCTGCCGCTTGACGAGCAGGACGACACCCACCAGCGTTTGCATACCTTCCTATAGTCCAAGGGGCACAACCCCTGCTTTGGTTCATAATGACGGAGCTGGCAGCCAGGGTGGGCGAGACGGCAGGGCGATTGGCTTg                <  1:4733963/150‑1 (MQ=255)

                                                                                                                                     gCGGCAAGCGGCGGCTGCCGCTTGACGAGCAGGACGACACCCACCAGCGTTTGCATACCTTCCTATAGTCCAAGGGGCACAACCCCTGCTTTGGTTCATAATGACGGAGCTGGCAGCCAGGGTGGGCGAGACGGCAGGGCGATTGGCTTg                <  2:4588177/150‑1 (MQ=255)

                                                                                                                                      cgtcAAGCGGCAGCCGCCGCTTGCCGAGCAGGACGACACCCACCAGCGTTTGCATACCTTCCTATAGTCCAAGGGGCACAACCCCTGCTTTGGTTCATAATGACGGAGCTGGCAGCCAGGGTGGGCGAGACGGCAg                             <  1:1161980/133‑1 (MQ=255)

                                                                                                                                      cgtcAAGCGGCAGCCGCCGCTTGCCGAGCAGGACGACACCCACCAGCGTTTGCATACCTTCCTATAGTCCAAGGGGCACAACCCCTGCTTTGGTTCATAATGACGGAGCTGGCAGCCAGGGTGGGCGAGACGGCAg                             >  2:1161980/4‑136 (MQ=255)

                                                                                                                                       ggCAAGCGGCGGCTGCCGCTTGACGAGCAGGACGACACCCACCAGCGTTTGCATACCTTCCTATAGTCCAAGGGGCACAACCCCTGCTTTGGTTCATAATGACGGAGCTGGCAGCCAGGGTGGGCGAGACGGCAGGGCGATTGGCTTGAt              >  2:4337655/1‑150 (MQ=255)

                                                                                                                                         cAAGCGGCGGCTGCCGCTTGACGAGCAGGACGACACCCACCAGCGTTTGCATACCTTCCTATAGTCCAAGGGGCACAACCCCTGCTTTGGTTCATAATGACGGAGCTGGCAGCCAGGGTGGGCGAGACGGCAGGGCGATTGGCTTGAtct            >  1:2565929/1‑150 (MQ=255)

                                                                                                                                          aaGCGGCAGCCGCCGCTTGACGAGCAGGACGACACCCACCAGCGTTTGCATACCTTCCTATAGTCCAAGGGGCACAACCCCTGCTTTGGTTCATAATGACGGAGCTGGCAGCCAGGGTGGGCGAGACGGCAGGGCGATTGGCTTGAtctc           >  1:1464946/1‑150 (MQ=255)

                                                                                                                                            gcggcaGCCGCCGCTTGACGAGCAGGACGACACCCACCAGCGTTTGCATACCTTCCTATAGTCCAAGGGGCACAACCCCTGCTTTGGTTCATAATGACGGAGCTGGCAGCCAGGGTGGGCGAGACGGCAGGGCGATTGGc                   >  1:1584766/1‑140 (MQ=255)

                                                                                                                                            gcggcaGCCGCCGCTTGACGAGCAGGACGACACCCACCAGCGTTTGCATACCTTCCTATAGTCCAAGGGGCACAACCCCTGCTTTGGTTCATAATGACGGAGCTGGCAGCCAGGGTGGGCGAGACGGCAGGGCGATTGGc                   <  2:1584766/140‑1 (MQ=255)

                                                                                                                                             cggcggCTGCCGCTTGACGAGCAGGACGACACCCACCAGCGTTTGCATACCTTCCTATAGTCCAAGGGGCACAACCCCTGCTTTGGTTCATAATGACGGAGCTGGCAGCCAGGGTg                                          <  2:5300584/116‑1 (MQ=255)

                                                                                                                                             cggcggCTGCCGCTTGACGAGCAGGACGACACCCACCAGCGTTTGCATACCTTCCTATAGTCCAAGGGGCACAACCCCTGCTTTGGTTCATAATGACGGAGCTGGCAGCCAGGGTg                                          >  1:5300584/1‑116 (MQ=255)

                                                                                                                                              ggcggcTGCCGCTTGACGAGCAGGACGACACCCACCAGCGTTTGCATACCTTCCTATAGTCCAAGGGGCACAACCCCTGCTTTGGTTCATAATGACGGAGCTGGCAGCCAGGGTGGGCGAGACGGCAGGGCGATTGGCTTGATCTCCCtt       <  1:4799596/150‑1 (MQ=255)

                                                                                                                                                   cTGCCGCTTGACGAGCAGGACGACACCCACCAGCGTTTGCATACCTTCCTATAGTCCAAGGGGCACAACCCCTGCTTTGGTTCATAATGACGGAGCTGGCAGCCAGGGTGGGCGAGACGGCAGGGCGATTGGCTTGATCTCCCTTGACCg  <  2:1867102/150‑1 (MQ=255)

CTGCGGCGCTCCAAGCGCGATGATCATTTCTGTACGGCAGAAGTTGCGGCGATGTGCCTGGACCTTGCTGGCGATGGCCAGGCTTCGCAGGCGCTGGATGCCTATCTGGATGTGTTCAGCCTGCATTACCTGAGCGGCAAGCGGCGGCTGCCGCTTGACGAGCAGGACGACACCCACCAGCGTTTGCATACCTTCCTATAGTCCAAGGGGCACAACCCCTGCTTTGGTTCATAATGACGGAGCTGGCAGCCAGGGTGGGCGAGACGGCAGGGCGATTGGCTTGATCTCCCTTGACCG  >  NC_002947/1831674‑1831970

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`