BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	100,116	T→G	5.6%	N98T (AAC→ACC)	PP_0094 ←	5'‑nucleotidase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	100,116	0	T	G	5.6%	691.5 / 17.0	220	N98T (AAC→ACC)	PP_0094	5'‑nucleotidase
Reads supporting (aligned to +/- strand): ref base T (106/100); new base G (9/5); total (115/105)
Fisher's exact test for biased strand distribution p-value = 4.16e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 221 total aligned reads displayed.

AATCAGCTCGACCTTGTTGGTGCGCGTGCCGTCCAGTTCACTGCCGTAGATCACCTTGAAGTGATGATCGAACGCAAAGTGCCGAGCGATTTCGCGGGCGAACTCCCATGGTTTGGAGGTGGCAATGTACAGCGTGCGGCCCTGCCCGTTCAGGGCCTCAAGCAACTCCGGCACACCTTCGAACACGAGGTTTTCGTAAAGACCGGTCACGCGGAAGCGCTCGCGGTAGAAGTTCACCGCATCCCAGGCCTTGGCTTCGTCGAAGCTGTAGAACTGCATGAAAGCCTGCAGCAGGGG  >  NC_002947/99968‑100264

 aTCAGCTCGACCTTGTTGGTGCGCGTGCCGTCCAGTTCACTGCCGTAGATCACCTTGAAGTGATGATCGAACGCAAAGTGCCGAGCGATTTCGCGGGCGAACTCCCATGGTTTGGAGGTGGCAATGTACAGCGTGCGGCCCTGCCCGGTc                                                                                                                                                    >  2:1686391/1‑150 (MQ=255)

    aGCTCGACCTTGTTGGTGCGCGTGCCGTCCAGTTCACTGCCGTAGATCACCTTGAAGTGATGATCGAACGCAAAGTGCCGAGCGATTTCGCGGGCGAACTCCCATGGTTTGGAGGTGGCAATGTACAGCGTGCGGCCCTGCCCGTTCAgg                                                                                                                                                 <  1:5099472/150‑1 (MQ=255)

    aGCTCGACCTTGTTGGTGCGCGTGCCGTCCAGTTCACTGCCGTAGATCACCTTGAAGTGATGATCGAACGCAAAGTGCCGAGCGATTTCGCGGGCGAACTCCCATGGTTTGGAGGTGGCAATGTACAGCGTGCGGCCCTGCCCGTTCAgg                                                                                                                                                 >  2:3868331/1‑150 (MQ=255)

     gCTCGACCTTGTTGGTGCGCGTGCCGTCAAGTTCACTGCCGTAGATCACCTTGAAGTGATGATCGAACGCAAAGTGCCGAGCGATTTCGCGGGCGAACTCCCATGGTTTGGAGGTGGCAATGTACAGCGAGCGGCCCTGCACGTTCAggg                                                                                                                                                >  1:4012702/1‑150 (MQ=255)

      cTCGACCTTGTTGGTGCGCGTGCCGTCCAGTTCACTGCCGTAGATCACCTTGAAGTGATGATCGAACGCAAAGTGCCGAGCGATTTCGCGGGCGAACTCCCATGGTTTGGAGGTGGCAATGTACAGCGTGCGGCCCTGAACGGGCAGGGc                                                                                                                                               <  1:4551077/150‑1 (MQ=255)

       tCGACCTTGTTGGTGCGCGTGCCGTCCAGTTCACTGCCGTAGATCACCTTGAAGTGATGATCGAACGCAAAGTGCCGAGCGATTTCGCGGGCGAACTCCCATGGTTTGGAGGTGGCAATGTACAGCGTGCGGCCCTGCCCGGTCAGGGcc                                                                                                                                              >  1:3809649/1‑150 (MQ=255)

         gACCTTGTTGGTGCGCGTGCCGTCCAGTTCACTGCCGTAGATCACCTTGAAGTGATGATCGAACGCAAAGTGCCGAGCGATTTCGCGGGCGAACTCCCATGGTTTGGAGGTGGCAATGTACAGCGTGCGGCCCTGCCCGTTCAGGGCCTc                                                                                                                                            >  1:4736059/1‑150 (MQ=255)

         gACCTTGTTGGTGCGCGTGCCGTCCAGTTCACTGCCGTAGATCACCTTGAAGTGATGATCGAACGCAAAGTGCCGAGCGATTTCGCGGGCGAACTCCCATGGTTTGGAGGTGGCAATGTACAGCGTGCGGCCCTGCCCGTTCAGGGCCTc                                                                                                                                            >  2:2118138/1‑150 (MQ=255)

           ccTTGTTGGTGCGCGTGCCGTCCAGTTCACTGCCGTAGATCACCTTGAAGTGATGATCGAACGCAAAGTGCCGAGCGATTTCGCGGGCGAACTCCCATGGTTTGGAGGTGGCAATGTACAGCGTGCGGCCCTGCCCGTTCAGGGCCTCaa                                                                                                                                          <  1:3169979/150‑1 (MQ=255)

           ccTTGTTGGTGCGCGTGCCGTCCAGTTCACTGCCGTAGATCACCTTGAAGTGATGATCGAACGCAAAGTGCCGAGCGATTTCGCGGGCGAACTCCCATGGTTTGGAGGTGGCAATGTACAGCGTGCGGCCCTGCCCGTTCAGGGCCTCaa                                                                                                                                          <  1:4400852/150‑1 (MQ=255)

           ccTTGTTGGTGCGCGTGCCGTCCAGTTCACTGCCGTAGATCACCTTGAAGTGATGATCGAACGCAAAGTGCCGAGCGATTTCGCGGGCGAACTCCCATGGTTTGGAGGTGGCAATGTACAGCGTGCGGCCCTGCCCGTTCAGGGCCTCaa                                                                                                                                          <  1:4500685/150‑1 (MQ=255)

           ccTTGTTGGTGCGCGTGCCGTCCAGTTCACTGCCGTAGATCACCTTGAAGTGATGATCGAACGCAAAGTGCCGAGCGATTTCGCGGGCGAACTCCCATGGTTTGGAGGTGGCAATGTACAGCGTGCGGCCCTGCCCGTTCAGGGCCTCaa                                                                                                                                          <  1:739889/150‑1 (MQ=255)

            cTTGTTGGTGCGCGTGCCGTCCAGTTCACTGCCGTAGATCACCTTGAAGTGATGATCGAACGCAAAGTGCCGAGCGATTTCGCGGGCGAACTCCCATGGTTTGGAGGTGGCAATGTACAGCGTGCGGCCCTGCCCGTTCAGGGCCTCAAg                                                                                                                                         <  2:937162/150‑1 (MQ=255)

            cTTGTTGGTGCGCGTGCCGTCCAGTTCACTGCCGTAGATCACCTTGAAGTGATGATCGAACGCAAAGTGCCGAGCGATTTCGCGGGCGAACTCCCATGGTTTGGAGGTGGCAATGTACAGCGTGAGGCCCTGAACGGGCAGGGCCGCa                                                                                                                                           <  2:4630483/148‑1 (MQ=255)

             ttgttgGTGCGCGTGCCGTCCAGTTCACTGCCGTAGATCACCTTGAAGTGATGATCGAACGCAAAGTGCCGAGCGATTTCGCGGGCGAACTCCCATGGTTTGGAGGTGGCAATGTACAGCGTGCGGCCCTGCCCGTTCAGGGCCTCAAGc                                                                                                                                        <  2:4556682/150‑1 (MQ=255)

             ttgttgGTGCGCGTGCCGTCCAGTTCACTGCCGTAGATCACCTTGAAGTGATGATCGAACGCAAAGTGCCGAGCGATTTCGCGGGCGAACTCCCATGGTTTGGAGGTGGCAATGTACAGCGTGCGGCCCTGCCCGTTCAGGGCCTCAAGc                                                                                                                                        <  2:5412923/150‑1 (MQ=255)

              ggtgggTGCGCGTGCCGTCCCGTCCCCGGCCGTAGATCACCTTGAAGTGAGGATCGACCGCAACGTGCCGCGCGATTTCGCGGGCGAACTCCCATGGTTTGGAGGTGGCAATGTCCCGCGTGCGGCCCTGACCGGGCCGGGCCGCACGct                                                                                                                                       <  2:429738/146‑2 (MQ=255)

                  ggTGCGCGTGCCGTCCAGTTCACTGCCGTAGATCACCTTGAAGTGATGATCGAACGCAAAGTGCCGAGCGATTTCGCGGGCGAACTCCCATGGTTTGGAGGTGGCAATGTACAGCGTGCGGCCCTGCCCGTTCAGGGCCTCAAGCAACTc                                                                                                                                   <  2:5330188/150‑1 (MQ=255)

                     gcgcGTGCCGTCCAGTTCACTGCCGTAGATCACCTTGAAGTGATGATCGAACGCAAAGTGCCGAGCGATTTCGCGGGCGAACTCCCATGGTTTGGAGGTGGCAATGTACAGCGTGCGGCCCTGCCCGTTCAGGGCCTCAAGCAACTCCgg                                                                                                                                <  2:5289112/150‑1 (MQ=255)

                     gcgcGTGCCGTCCAGTTCACTGCCGTAGATCACCTTGAAGTGATGATCGAACGCAAAGTGCCGAGCGATTTCGCGGGCGAACTCCCATGGTTTGGAGGTGGCAATGTACAGCGTGCGGCCCTGCCCGTTCAGGGCCTCAAGCAACTCCgg                                                                                                                                <  1:805402/150‑1 (MQ=255)

                      cgcgTGCCGTCCAGTTCACTGCCGTAGATCACCTTGAAGTGATGATCGAACGCAAAGTGCCGAGCGATTTCGCGGGCGAACTCCCATGGTTTGGAGGTGGCAATGTACAGCGTGCGGCCCTGCCCGTTCAGGGCCTCAAGCAACTCCGGc                                                                                                                               <  2:2426514/150‑1 (MQ=255)

                             cGTCCAGTTCACTGCCGTAGATCACCTTGAAGTGATGATCGAACGCAAAGTGCCGAGCGATTTCGCGGGCGAACTCCCATGGTTTGGAGGTGGCAATGTACAGCGTGCGGCCCTGCCCGTTCAGGGCCTCAAGCAACTCCGGCACACCtt                                                                                                                        <  2:4377395/150‑1 (MQ=255)

                             cGTCCAGTTCACTGCCGTAGATCACCTTGAAGTGATGATCGAACGCAAAGTGCCGAGCGATTTCGCGGGCGAACTCCCATGGTTTGGAGGTGGCAATGTACAGCGTGCGGCCCTGCCCGTTCAGGGCCTCAAGCAACTCCGGCACACCtt                                                                                                                        >  2:469360/1‑150 (MQ=255)

                                    ttCACTGCCGTAGATCACCTTGAAGTGATGATCGAACGCAAAGTGCCGAGCGATTTCGCGGGCGAACTCCCATGGTTTGGAGGTGGCAATGTACAGCGTGCGGCCCTGCCCGTTCAGGGCCTCAAGCAACTCCGGCACACCTTCGAacac                                                                                                                 >  1:3019248/1‑150 (MQ=255)

                                    ttCACTGCCGTAGATCACCTTGAAGTGATGATCGAACGCAAAGTGCCGAGCGATTTCGCGGGCGAACTCCCATGGTTTGGAGGTGGCAATGTACAGCGTGCGGCCCTGCCCGTTCAGGGCCTCAAGCAACTCCGGCACACCTTCGAacac                                                                                                                 >  2:558768/1‑150 (MQ=255)

                                    ttCACTGCCGTAGATCACCTTGAAGTGATGATCGAACACAAAGTGCCGAGCGATTTCGCGGGCGAACTCCCATGGTTTGGAGGTGGCAATGTACAGCGTGCGGCCCTGCCCGTTCAGGGCCTCAAGCAACTCCGGCACACCTTCGAacac                                                                                                                 <  2:4850581/150‑1 (MQ=255)

                                      cACTGCCGTAGATCACCTTGAAGTGATGATCGAACGCAAAGTGCCGAGCGATTTCGCGGGCGAACTCCCATGGTTTGGAGGTGGCAATGTACAGCGTGCGGCCCTGCCCGTTCAGGGCCTCAAGCAACTCCGGCACACCTTCGAACACGa                                                                                                               <  2:2311947/150‑1 (MQ=255)

                                       aCTGCCGTAGATCACCTTGAAGTGATGATCGAACGCAAAGTGCCGAGCGATTTCGCGGGCGAACTCCCATGGTTTGGAGGTGGCAATGTACAGCGTGCGGCCCTGCCCGTTCAGGGCCTCAAGCAACTCCGGCACACCTTCGAACACGAg                                                                                                              <  1:2310080/150‑1 (MQ=255)

                                         tGCCGTAGATCACCTTGAAGTGATGATCGAACGCAAAGTGCCGAGCGATTTCGCGGGCGAACTCCCATGGTTTGGAGGTGGCAATGTACAGCGTGCGGCCCTGCCCGTTCAGGGCCTCAAGCAACTCCGGCACACCTTCGAACACGAGGt                                                                                                            >  2:5011543/1‑150 (MQ=255)

                                         tGCCGTAGATCACCTTGAAGTGATGATCGAACGCAAAGTGCCGAGCGATTTCGCGGGCGAACTCCCATGGTTTGGAGGTGGCAATGTACAGCGTGCGGCCCTGCCCGTTCAGGGCCTCAAGCAACTCCGGCACACCTTCGAACACGAGGt                                                                                                            >  2:2282269/1‑150 (MQ=255)

                                         tGCCGTAGATCACCTTGAAGTGATGATCGAACGCAAAGTGCCGAGCGATTTCGCGGGCGAACTCCCATGGTTTGGAGGTGGCAATGTACAGCGTGCGGCCCTGCCCGTTCAGGGCCTCAAGCAACTACGGCACACCTTCCAACACGAGGt                                                                                                            >  1:1015/1‑150 (MQ=255)

                                          gCCGTAGATCACCTTGAAGTGATGATCGAACGCAAAGTGCCGAGCGATTTCGCGGGCGAACTCCCATGGTTTGGAGGTGGCAATGTACAGCGTGCGGCCCTGCCCGTTCAGGGCCTCAAGCAACTCCGGCACACCTTCGAACACGAGGtt                                                                                                           >  1:2120248/1‑150 (MQ=255)

                                          gCCGTAGATCACCTTGAAGTGATGATCGAACGCAAAGTGCCGAGCGATTTCGCGGGCGAACTCCCATGGTTTGGAGGTGGCAATGTACAGCGTGCGGCCCTGCCCGTTCAGGGCCTCAAGCAACTCCGGCACACCTTCGAACACGAGGtt                                                                                                           >  1:4855976/1‑150 (MQ=255)

                                           ccGTAGATCACCTTGAAGTGATGATCGAACGCAAAGTGCCGAGCGATTTCGCGGGCGAACTCCCATGGTTTGGAGGTGGCAATGTACAGCGTGCGGCCCTGCCCGTTCAGGGCCTCAAGCAACTCCGGCACACCTTCGAACACGAGGttt                                                                                                          >  2:3397647/1‑150 (MQ=255)

                                           ccGTAGATCACCTTGAAGTGATGATCGAACGCAAAGTGCCGAGCGATTTCGCGGGCGAACTCCCATGGTTTGGAGGTGGCAATGTACAGCGTGCGGCCCTGCCCGTTCAGGGCCTCAAGCAACTCCGGCACACCTTCGAACACGAGGttt                                                                                                          >  2:2890750/1‑150 (MQ=255)

                                           ccGTAGATCACCTTGAAGTGATGATCGAACGCAAAGTGCCGAGCGATTTCGCGGGCGAACTCCCATGGTTTGGAGGTGGCAATGTACAGCGTGCGGCCCTGCCCGTTCAGGGCCTCAAGCAACTCCGGCACACCTTCGAACACGAGGttt                                                                                                          >  1:3244884/1‑150 (MQ=255)

                                           ccGTAGATCACCTTGAAGTGATGATCGAACGCAAAGTGCCGAGCGATTTCGCGGGCGAACTCCCATGGTTTGGAGGTGGCAATGTACAGCGTGCGGCCCTGCCCGTTCAGGGCCTCAAGCAACTCCGGCACACCTTCGAACACGAGGttt                                                                                                          >  2:1003753/1‑150 (MQ=255)

                                           ccGTAGATCACCTTGAAGTGATGATCGAACGCAAAGTGCCGAGCGATTTCGCGGGCGAACTCCCATGGTTTGGAGGTGGCAATGTACAGCGTGCGGCCCTGCCCGTTCAGGGCCTCAAGCAACTCCGGCACACCTTCGAACACGAGGttt                                                                                                          <  1:5420120/150‑1 (MQ=255)

                                             gTAGATCACCTTGAAGTGATGATCGAACGCAAAGTGCCGAGCGATTTCGCGGGCGAACTCCCATGGTTTGGAGGTGGCAATGTACAGCGTGCGGCCCTGCCCGTTCAGGGCCTCAAGCAACTCCGGCACACCTTCGAACACGAGGTTTTc                                                                                                        >  1:4813093/1‑150 (MQ=255)

                                             gTAGATCACCTTGAAGTGATGATCGAACGCAAAGTGCCGAGCGATTTCGCGGGCGAACTCCCATGGTTTGGAGGTGGCAATGTACAGCGTGCGGCCCTGCCCGTTCAGGGCCTCAAGCAACTCCGGCACACCTTCGAACACGAGGTTTTc                                                                                                        >  2:2597580/1‑150 (MQ=255)

                                             gTAGATCACCTTGAAGTGATGATCGAACGCAAAGTGCCGAGCGATTTCGCGGGCGAACTCCCATGGTTTGGAGGTGGCAATGTACAGCGTGCGGCCCTGCCCGTTCAGGGCCTCAAGCAACTCCGGCACACCTTCGAACACGAGGTTTTc                                                                                                        <  2:3045579/150‑1 (MQ=255)

                                             gTAGATCACCTTGAAGTGATGATCGAACGCAAAGTGCCGAGCGATTTCGCGGGCGAACTCCCATGGTTTGGAGGTGGCAATGTACAGCGTGCGGCCCTGCCCGTTCAGGGCCTCAAGCAACTCCGGCACACCTTCGAACACGAGGTTTTc                                                                                                        <  1:958140/150‑1 (MQ=255)

                                                gATCACCTTGAAGTGATGATCGAACGCAAAGTGCCGAGCGATTTCGCGGGCGAACTCCCATGGTTTGGAGGTGGCAATGTACAGCGTGCGGCCCTGCCCGTTCAGGGCCTCAAGCAACTCCGGCACACCTTCGAACACGAGGTTTTCGTa                                                                                                     <  1:2058986/150‑1 (MQ=255)

                                                gATCACCTTGAAGTGATGATCGAACGCAAAGTGCCGAGCGATTTCGCGGGCGAACTCCCATGGTTTGGAGGTGGCAATGTACAGCGTGCGGCCCTGCCCGTTCAGGGCCTCAAGCAACTCCGGCACACCTTCGAACACGAGGTTTTCGTa                                                                                                     <  1:4176993/150‑1 (MQ=255)

                                                  tCACCTTGAAGTGATGATCGAACGCAAAGTGCCGAGCGATTTCGCGGGCGAACTCCCATGGTTTGGAGGTGGCAATGTACAGCGTGCGGCCCTGCCCGTTCAGGGCCTCAAGCAACTCCGGCACACCTTCGAACACGAGGTTTTCGTaaa                                                                                                   <  2:2731432/150‑1 (MQ=255)

                                                  tCACCTTGAAGTGATGATCGAACGCAAAGTGCCGAGCGATTTCGAGGTCGAACTCCCATGGTTTGGAGGTGGCAATGTACTGCGTGCGGACCTGCCCGTTGAGGGCCTCATGCTACTCCGTCACTCATTCGAACTCGAGGTTTTCGTaaa                                                                                                   <  2:2639709/150‑1 (MQ=255)

                                                     ccTTGAAGTGATGATCGAACGCAAAGTGCCGAGCGATTTCGCGGGCGAACTCCCATGGTTTGGAGGTGGCAATGTACAGCGTGCGGCCCTGCCCGTTCAGGGCCTCAAGCAACTCCGGCACACCTTCGAACACGAGGTTTTCGTAAAGAc                                                                                                >  1:2466794/1‑150 (MQ=255)

                                                      cTTGAAGTGATGATCGAACGCAAAGTGCCGAGCGATTTCGCGGGCGAACTCCCATGGTTTGGAGGTGGCAATGTACAGCGTGCGGCCCTGCCCGTTCAGGGCCTCAAGCAACTCCGGCACACCTTCGAACACGAGGTTTTCGTAAAGAcc                                                                                               >  2:3702277/1‑150 (MQ=255)

                                                                tgatCGAACGCAAAGTGCCGAGCGATTTCGCGGGCGAACTCCCATGGTTTGGAGGTGGCAATGTACAGCGTGCGGCCCTGCCCGTTCAGGGCCTCAAGCAACTCCGGCACACCTTCGAACACGAGGTTTTCGTAAAGACCGGTCACGCgg                                                                                     >  1:4611528/1‑150 (MQ=255)

                                                                  atCGAACGCAAAGTGCCGAGCGATTTCGCGGGCGAACTCCCATGGTTTGGAGGTGGCAATGTACAGCGTGCGGCCCTGCCCGTTCAGGGCCTCAAGCAACTCCGGCACACCTTCGAACACGAGGTTTTCGTAAAGACCGGTCACGCGGaa                                                                                   <  1:2837598/150‑1 (MQ=255)

                                                                   tCGAACGCAAAGTGCCGAGCGATTTCGCGGGCGAACTCCCATGGTTTGGAGGTGGCAATGTACAGCGTGCGGCCCTGCCCGTTCAGGGCCTCAAGCAACTCCGGCACACCTTCGAACACGAGGTTTTCGTAAAGACCGGTCACGCGGAAg                                                                                  >  2:1448512/1‑150 (MQ=255)

                                                                      aaCGCAAAGTGCCGAGCGATTTCGCGGGCGAACTCCCATGGTTTGGAGGTGGCAATGTACAGCGTGCGGCCCTGCCCGTTCAGGGCCTCAAGCAACTCCGGCACACCTTCGAACACGAGGTTTTCGTAAAGACCGGTCACGCGGAAgcgc                                                                               >  1:667651/1‑150 (MQ=255)

                                                                       aCGCAAAGTGCCGAGCGATTTCGCGGGCGAACTCCCATGGTTTGGAGGTGGCAATGTACAGCGTGCGGCCCTGCCCGTTCAGGGCCTCAAGCAACTCCGGCACACCTTCGAACACGAGGTTTTCGTAAAGACCGGTCACGCGGAAGCGCt                                                                              >  1:2700379/1‑150 (MQ=255)

                                                                         gCAAAGTGCCGAGCGATTTCGCGGGCGAACTCCCATGGTTTGGAGGTGGCAATGTACAGCGTGCGGCCCTGCCCGTTCAGGGCCTCAAGCAACTCCGGCACACCTTCGAACACGAGGTTTTCGTAAAGACCGGTCACGCGGAAGCGCTcg                                                                            >  1:1304904/1‑150 (MQ=255)

                                                                          cAAAGTGCCGAGCGATTTCGCGGGCGAACTCCCATGGTTTGGAGGTGGCAATGTACAGCGTGCGGCCCTGCCCGTTCAGGGCCTCAAGCAACTCCGGCACACCTTCGAACACGAGGTTTTCGTAAAGACCGGTCACGCGGAAGCGCTcgc                                                                           >  2:2172948/1‑150 (MQ=255)

                                                                                gCCGAGCGATTTCGCGGGCGAACTCCCATGGTTTGGAGGTGGCAATGTACAGCGTGCGGCCCTGCCCGTTCAGGGCCTCAAGCAACTCCGGCACACCTTCGAACACGAGGTTTTCGTAAAGACCGGTCACGCGGAAGCGCTCGCGGTAGa                                                                     >  1:2099045/1‑150 (MQ=255)

                                                                                 ccGAGCGATTTCGCGGGCGAACTCCCATGGTTTGGAGGTGGCAATGTACAGCGTGCGGCCCTGCCCGTTCAGGGCCTCAAGCAACTCCGGCACACCTTCGAACACGAGGTTTTCGTAAAGACCGGTCACGCGGAAGCGCTCGCGGTAGaa                                                                    <  2:3072316/150‑1 (MQ=255)

                                                                                       gATTTCGCGGGCGAACTCCCATGGTTTGGAGGTGGCAATGTACAGCGTGCGGCCCTGCCCGTTCAGGGCCTCAAGCAACTCCGGCACACCTTCGAACACGAGGTTTTCGTAAAGACCGGTCACGCGGAAGCGCTCGCGGTAGAAGTTCAc                                                              <  2:3244884/150‑1 (MQ=255)

                                                                                          ttCGCGGGCGAACTCCCATGGTTTGGAGGTGGCAATGTACAGCGTGCGGCCCTGCCCGTTCAGGGCCTCAAGCAACTCCGGCACACCTTCGAACACGAGGTTTTCGTAAAGACCGGTCACGCGGAAGCGCTCGCGGTAGAAGTTCACCGc                                                           <  2:4314348/150‑1 (MQ=255)

                                                                                             gcgGGCGAACTCCCATGGTTTGGAGGTGGCAATGTACAGCGTGCGGCCCTGCCCGTTCAGGGCCTCAAGCAACTCCGGCACACCTTCGAACACGAGGTTTTCGTAAAGACCGGTCACGCGGAAGCGCTCGCGGTAGAAGTTCACCGCATc                                                        >  1:4739034/1‑150 (MQ=255)

                                                                                                ggCGAACTCCCATGGTTTGGAGGTGGCAATGTACAGCGTGCGGCCCTGCCCGTTCAGGGCCTCAAGCAACTCCGGCACACCTTCGAACACGAGGTTTTCGTAAAGACCGGTCACGCGGAAGCGCTCGCGGTAGAAGTTCACCGCATCCCa                                                     <  1:533444/150‑1 (MQ=255)

                                                                                                 gCGAACTCCCATGGTTTGGAGGTGGCAATGTACAGCGTGCGGCCCTGCCCGTTCAGGGCCTCAAGCAACTCCGGCACACCTTCGAACACGAGGTTTTCGTAAAGACCGGTCACGCGGAAGCGCTCGCGGTAGAAGTTCACCGCATCCCAg                                                    <  1:1990819/150‑1 (MQ=255)

                                                                                                   gAACTCCCATGGTTTGGAGGTGGCAATGTACAGCGTGCGGCCCTGCCCGTTCAGGGCCTCAAGCAACTCCGGCACACCTTCGAACACGAGGTTTTCGTAAAGACCGGTCACGCGGAAGCGCTCGCGGTAGAAGTTCACCGCATCCCAGGc                                                  >  2:4779283/1‑150 (MQ=255)

                                                                                                       tCCCATGGTTTGGAGGTGGCAATGTACAGCGTGCGGCCCTGCCCGTTCAGGGCCTCAAGCAACTCCGGCACACCTTCGAACACGAGGTTTTCGTAAAGACCGGTCACGCGGAAGCGCTCGCGGTAGAAGTTCACCGCATCCCAGGCCTTg                                              >  1:1113851/1‑150 (MQ=255)

                                                                                                       tCCCATGGTTTGGAGGTGGCAATGTACAGCGTGCGGCCCTGCCCGTTCAGGGCCTCAAGCAACTCCGGCACACCTTCGAACACGAGGTTTTCGTAAAGACCGGTCACGCGGAAGCGCTCGCGGTAGAAGTTCACCGCATCCCAGGCCTTg                                              >  1:4090994/1‑150 (MQ=255)

                                                                                                       tCCCATGGTTTGGAGGTGGCAATGTACAGCGTGCGGCCCTGCCCGTTCAGGGCCTCAAGCAACTCCGGCACACCTTCGAACACGAGGTTTTCGTAAAGACCGGTCACGCGGAAGCGCTCGCGGTAGAAGTTCACCGCATCCCAGGCCATg                                              >  1:3937943/1‑150 (MQ=255)

                                                                                                        cccATGGTTTGGAGGTGGCAATGTACAGCGTGCGGCCCTGCCCGTTCAGGGCCTCAAGCAACTCCGGCACACCTTCGAACACGAGGTTTTCGTAAAGACCGGTCACGCGGAAGCGCTCGCGGTAGAAGTTCACCGCATCCCAGGCCTTgg                                             <  2:528681/150‑1 (MQ=255)

                                                                                                         ccATGGTTTGGAGGTGGCAATGTACAGCGTGCGGCCCTGCCCGTTCAGGGCCTCAAGCAACTCCGGCACACCTTCGAACACGAGGTTTTCGTAAAGACCGGTCACGCGGAAGCGCTCGCGGTAGAAGTTCACCGCATCCCAGGCCTTgac                                            >  2:1202997/1‑148 (MQ=255)

                                                                                                         ccATGGTTTGGAGGTGGCAATGTACAGCGTGCGGCCCTGCCCGTTCAGGGCCTCAAGCAACTCCGGCACACCTTCGAACACGAGGTTTTCGTAAAGACCGGTCACGCGGAAGCGCTCGCGGTAGAAGTTCACCGCATCCCAGGCCTTGGc                                            >  1:76579/1‑150 (MQ=255)

                                                                                                         ccATGGTTTGGAGGTGGCAATGTACAGCGTGCGGCCCTGCCCGTTCAGGGCCTCAAGCAACTCCGGCACACCTTCGAACACGAGGTTTTCGTAAAGACCGGTCACGCGGAAGCGCTCGCGGTAGAAGTTCACCGCATCCCAGGCCTTGGc                                            >  1:4792737/1‑150 (MQ=255)

                                                                                                          cATGGTTTGGAGGTGGCAATGTACAGCGTGCGGCCCTGCCCGTTCAGGGCCTCAAGCAACTCCGGCACACCTTCGAACACGAGGTTTTCGTAAAGACCGGTCACGCGGAAGCGCTCGCGGTAGAAGTTCACCGCATCCCAGGCCTTGGCt                                           <  1:625468/150‑1 (MQ=255)

                                                                                                              gTTTGGAGGTGGCAATGTACAGCGTGCGGCCCTGCCCGTTCAGGGCCTCAAGCAACTCCGGCACACCTTCGAACACGAGGTTTTCGTAAAGACCGGTCACGCGGAAGCGCTCGCGGTAGAAGTTCACCGCATCCCAGGCCTTGGCTtcgt                                       <  1:601977/150‑1 (MQ=255)

                                                                                                                   gAGGTGGCAATGTACAGCGTGCGGCCCTGCCCGTTCAGGGCCTCAAGCAACTCCGGCACACCTTCGAACACGAGGTTTTCGTAAAGACCGGTCACGCGGAAGCGCTCGCGGTAGAAGTTCACCGCATCCCAGGCCTTGGCTTCGTCGAAg                                  >  1:1923170/1‑150 (MQ=255)

                                                                                                                   gAGGTGGCAATGTACAGCGTGCGGCCCTGCCCGGTCAGGGCCTCAAGCAACTCCGGCACACCTTCGAACACGAGGTTTTCGTAAAGACCGGTCACGCGGAAGCGCTCGCGGTAGAAGTTCACCGCATCCCAGGCCTTGGCTTCGTCGAAg                                  >  1:3416369/1‑150 (MQ=255)

                                                                                                                      gTGGCAATGTACAGCGTGCGGCCCTGCCCGTTCAGGGCCTCAAGCAACTCCGGCACACCTTCGAACACGAGGTTTTCGTAAAGACCGGTCACGCGGAAGCGCTCGCGGTAGAAGTTCACCGCATCCCAGGCCTTGGCTTCGTCGAAGCTg                               <  2:4927218/150‑1 (MQ=255)

                                                                                                                      gTGGCAATGTACAGCGTGCGGCCCTGCCCGTTCAGGGCCTCAAGCAACTCCGGCACACCTTCGAACACGAGGTTTTCGTAAAGACCGGTCACGCGGAAGCGCTCGCGGTAGAAGTTCACCGCATCCCAGGCCTTGGCTTCGTCGAAGCTg                               <  2:2964890/150‑1 (MQ=255)

                                                                                                                      gTGGCAATGTACAGCGTGCGGCCCTGCCCGTTCAGGGCCTCAAGCAACTCCGGCACACCTTCGAACACGAGGTTTTCGTAAAGACCGGTCACGCGGAAGCGCTCGCGGTAGAAGTTCACCGCATCCCAGGCCTTGGCTTCGTCGAAGCTg                               >  1:36038/1‑150 (MQ=255)

                                                                                                                      gTGGCAATGTACAGCGTGCGGCCCTGCCCGTTCAGGGCCTCAAGCAACTCCGGCACACCTTCGAACACGAGGTTTTCGTAAAGACCGGTCACGCGGAAGCGCTCGCGGTAGAAGTTCACCGCATCCCAGGCCTTGGCTGCGTCGAAGCTg                               >  1:2763382/1‑150 (MQ=255)

                                                                                                                       tGGCAATGTACAGCGTGCGGCCCTGCCCGTTCAGGGCCTCAAGCAACTCCGGCACACCTTCGAACACGAGGTTTTCGTAAAGACCGGTCACGCGGAAGCGCTCGCGGTAGAAGTTCACCGCATCCCAGGCCTTGGCTTCGTCGAAGCTGt                              <  1:1686568/150‑1 (MQ=255)

                                                                                                                           aaTGTACAGCGTGCGGCCCTGCCCGTTCAGGGCCTCAAGCAACTCCGGCACACCTTCGAACACGAGGTTTTCGTAAAGACCGGTCACGCGGAAGCGCTCGCGGTAGAAGTTCACCGCATCCCAGGCCTTGGCTtcgt                                       <  1:854261/137‑1 (MQ=255)

                                                                                                                           aaTGTACAGCGTGCGGCCCTGCCCGTTCAGGGCCTCAAGCAACTCCGGCACACCTTCGAACACGAGGTTTTCGTAAAGACCGGTCACGCGGAAGCGCTCGCGGTAGAAGTTCACCGCATCCCAGGCCTTGGCTtcgt                                       >  2:854261/1‑137 (MQ=255)

                                                                                                                            aTGTACAGCGTGCGGCCCTGCCCGTTCAGGGCCTCAAGCAACTCCGGCACACCTTCGAACACGAGGTTTTCGTAAAGACCGGTCACGCGGAAGCGCTCGCGGTAGAAGTTCACCGCATCCCAGGCCTTGGCTTCGTCGAAGCTGTAGAAc                         <  1:4646225/150‑1 (MQ=255)

                                                                                                                             tGTACAGCGTGCGGCCCTGCCCGTTCAGGGCCTCAAGCAACTCCGGCACACCTTCGAACACGAGGTTTTCGTAAAGACCGGTCACGCGGAAGCGCTCGCGGTAGAAGTTCACCGCATCCCAGGCCTTGGCTTCGTCGAAGCTGTAGAACt                        <  1:1901109/150‑1 (MQ=255)

                                                                                                                             tGTACAGCGTGCGGCCCTGCCCGTTCAGGGCCTCAAGCAACTCCGGCACACCTTCGAACACGAGGTTTTCGTAAAGACCGGTCACGCGGAAGCGCTCGCGGTAGAAGTTCACCGCATCCCAGGCCTTGGCTTCGTCGAAGCTGTAGAACt                        <  2:2763083/150‑1 (MQ=255)

                                                                                                                             tGTACAGCGTGCGGCCCTGCCCGTTCAGGGCCTCAAGCAACTCCGGCACACCTTCGAACACGAGGTTTTCGTAAAGACCGGTCACGCGGAAGCGCTCGCGGTAGAAGTTCACCGCATCCCAGGCCTTGGCTTCGTCGAAGCTGTAGAACt                        <  2:460475/150‑1 (MQ=255)

                                                                                                                                aCAGCGTGCGGCCCTGCCCGTTCAGGGCCTCAAGCAACTCCGGCACACCTTCGAACACGAGGTTTTCGTAAAGACCGGTCACGCGGAAGCGCTCGCGGTAGAAGTTCACCGCATCCCAGGCCTTGGCTTCGTCGAAGCTGTGGAACTGCa                     >  1:2956550/1‑150 (MQ=255)

                                                                                                                                aCAGCGTGCGGCCCTGCCCGTTCAGGGCCTCAAGCAACTCCGGCACACCTTCGAACACGAGGTTTTCGTAAAGACCGGTCACGCGGAAGCGCTCGCGGTAGAAGTTCACCGCATCCCAGGCCTTGGCTTCGTCGAAGCTGTAGAACTGCa                     >  1:498987/1‑150 (MQ=255)

                                                                                                                                   gCGTGCGGCCCTGCCCGTTCAGGGCCTCAAGCAACTCCGGCACACCTTCGAACACGAGGTTTTCGTAAAGACCGGTCACGCGGAAGCGCTCGCGGTAGAAGTTCACCGCATCCCAGGCCTTGGCTTCGTCGAAGCTGTAGAACTGCATGa                  <  2:5253981/150‑1 (MQ=255)

                                                                                                                                       gCGGCCCTGCCCGTTCAGTGCCTCTAGCAACTCCTGCACACCTTCGAACACGAGGTTTTCGTAAAGACCGGTCACGCGGAAGCGCTCGCGGTAGAAGTTCACCGCATCCCAGGCCTTGGCTACGTCGAAGCTGTAGAACTGCATGAAAGc              <  2:3142810/150‑1 (MQ=255)

                                                                                                                                       gCGGCCCTGCCCGTTCAGGGCCTCAAGCAACTCCGGCACACCTTCGAACACGAGGTTTTCGTAAAGACCGGTCACGCGGAAGCGCTCGCGGTAGAAGTTCACCGCATCCCAGGCCTTGGCTTCGTCGAAGCTGTAGAACTGCATGAAAGc              >  1:1664272/1‑150 (MQ=255)

                                                                                                                                       gCGGCCCTGCCCGTTCAGGGCCTCAAGCAACTCCGGCACACCTTCGAACACGAGGTTTTCGTAAAGACCGGTCACGCGGAAGCGCTCGCGGTAGAAGTTCACCGCATCCCAGGCCTTGGCTTCGTCGAAGCTGTAGAACTGCATGAAAGc              <  1:1448512/150‑1 (MQ=255)

                                                                                                                                         ggCCCTGCCCGTTCAGGGCCTCAAGCAACTCCGGCACACCTTCGAACACGAGGTTTTCGTAAAGACCGGTCACGCGGAAGCGCTCGCGGTAGAAGTTCACCGCATCCCAGGCCTTGGCTTCGTCGAAGCTGTAGAACTGCATGAAAGCCt            <  1:5221739/150‑1 (MQ=255)

                                                                                                                                          gCCCTGCCCGTTCAGGGCCTCAAGCAACTCCGGCACACCTTCGAACACGAGGTTTTCGTAAAGACCGGTCACGCGGAAGCGCTCGCGGTAGAAGTTCACCGCATCCCAGGCCTTGGCTTCGTCGAAGCTGTAGAACTGCATGAAAGCCTg           <  2:3480712/150‑1 (MQ=255)

                                                                                                                                          gCCCTGCCCGTTCAGGGCCTCAAGCAACTCCGGCACACCTTCGAACACGAGGTTTTCGTAAAGACCGGTCACGCGGAAGCGCTCGCGGTAGAAGTTCACCGCATCCCAGGCCTTGGCTTCGTCGAAGCTGTAGAACTGCATGAAAGCCTg           <  2:1791947/150‑1 (MQ=255)

                                                                                                                                              tGCCCGTTCAGGGCCTCAAGCAACTCCGGCACACCTTCGAACACGAGGTTTTCGTAAAGACCGGTCACGCGGAAGCGCTCGCGGTAGAAGTTCACCGCATCCCAGGCCTTGGCTTCGTCGAAGCTGTAGAACTGCATGAAAGCCTgcagc       >  2:5091407/1‑150 (MQ=255)

                                                                                                                                               gCCCGTTCAGGGCCTCAAGCAACTCCGGCACACCTTCGAACACGAGGTTTTCGTAAAGACCGGTCACGCGGAAGCGCTCGCGGTAGAAGTTCACCGCATCCCAGGCCTTGGCTTCGTCGAAGCTGTAGAACTGCATGAAAGCCTgcagca      >  2:824462/1‑150 (MQ=255)

                                                                                                                                                  cGTTCAGGGCCTCAAGCAACTCCGGCACACCTTCGAACACGAGGTTTTCGTAAAGACCGGTCACGCGGAAGCGCTCGCGGTAGAAGTTCACCGCATCCCAGGCCTTGGCTTCGTCGAAGCTGTAGAACTGCATGAAAGCCTGCAGCAggg   <  1:1044978/150‑1 (MQ=255)

                                                                                                                                                   gTTTAGGGCCTCAAGCAACTCCGGCACACCTTCGAACACGAGGTTTTCGTAAAGACCGGTCACGCGGAAGCGCTCGCGGTAGAAGTTCACCGCATCCCAGGCCTTGGCTTCGTCGAAg                                  <  2:5085711/118‑1 (MQ=255)

                                                                                                                                                   gTTTAGGGCCTCAAGCAACTCCGGCACACCTTCGAACACGAGGTTTTCGTAAAGACCGGTCACGCGGAAGCGCTCGCGGTAGAAGTTCACCGCATCCCAGGCCTTGGCTTCGTCGAAg                                  >  1:5085711/1‑118 (MQ=255)

                                                                                                                                                   gTTCAGGGCCTCAAGCAACTCCGGCACACCTTCGAACACGAGGTTTTCGTAAAGACCGGTCACGCGGAAGCGCTCGCGGTAGAAGATCACCGCATCCCAGGCCTTGGCTTCGTCGAAGCTGTAGAACTGCATGAAAGCCTGCAGCAgggg  >  2:4183123/1‑150 (MQ=255)

AATCAGCTCGACCTTGTTGGTGCGCGTGCCGTCCAGTTCACTGCCGTAGATCACCTTGAAGTGATGATCGAACGCAAAGTGCCGAGCGATTTCGCGGGCGAACTCCCATGGTTTGGAGGTGGCAATGTACAGCGTGCGGCCCTGCCCGTTCAGGGCCTCAAGCAACTCCGGCACACCTTCGAACACGAGGTTTTCGTAAAGACCGGTCACGCGGAAGCGCTCGCGGTAGAAGTTCACCGCATCCCAGGCCTTGGCTTCGTCGAAGCTGTAGAACTGCATGAAAGCCTGCAGCAGGGG  >  NC_002947/99968‑100264

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 11 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`