Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 1,889,401 A→T 7.3% L305Q (CTG→CAG) ‡ PP_1695 ← sodium‑solute symporter/two‑component system sensor histidine kinase/response regulator

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029471,889,4010AT 7.3% 539.2 / 23.1 164L305Q (CTG→CAG) ‡PP_1695sodium‑solute symporter/two‑component system sensor histidine kinase/response regulator
Reads supporting (aligned to +/- strand):  ref base A (86/66);  new base T (7/5);  total (93/71)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.99e-01

Only 100 of 180 total aligned reads displayed.

CCATGGTCGACAACGCCACGGCCTCGACGATCACCATGCCGGTAGCCGCCGAGGCACCGCCGATGAACGCCAGCAGGGCAAGGCTCGGGTGCGCCTCGGCCAGGGGCAGGCTGATGACGAACGAGTCGGAGATCACCGTGCCGGGCAGCAGCATCTGCCCGGCCAGGGCAATCGGCACCACGAACAGCGCGGCCAGTGCCAGGTACATCGGGAATACCCAGCGCGCCAGGCGCAAGTCCTGCGGTTCGATGTTCTCCACCACGGTAACGTGGAACTGTCGGGGCAGGCAGATGATTGCC  >  NC_002947/1889252‑1889550
                                                                                                                                                     |                                                                                                                                                     
ccATGGTCGACAACGCCACGGCCTCGACGATCACCATGCCGGTAGCCGCCGAGGCACCGCCGATGAACGCCAGCAGGGCAAGGCTCGGGTGCGCCTCGGCCAGGGGCAGGCTGATGACGAACGAGTCGGAGATCACCGTGCCGGgcagca                                                                                                                                                       <  1:1114545/150‑1 (MQ=255)
 cATGGTCGACAACGCCACGGCCTCGACGATCACCATGCCGGTAGCCGCCGAGGCACCGCCGATGAACGCCAGCAGGGCAAGGCTCGGGTGCGCCTCGGCCAGGGGCAGGCTGATGACGAACGAGTCGGAGATCACCGTGCCGGgcagcag                                                                                                                                                      >  2:670079/1‑150 (MQ=255)
 cATGGTCGACAACGCCACGGCCTCGACGATCACCATGCCGGTAGCCGCCGAGGCACCGCCGATGAACGCCAGCAGGGCAAGGCTCGGGTGCGCCTCGGCCAGGGGCAGGCTGATGACGAACGAGTCGGAGATCACCGTGCCGGgcagcag                                                                                                                                                      >  1:3294859/1‑150 (MQ=255)
 cATGGTCGACAACGCCACGGCCTCGACGATCACCATGCCGGTAGCCGCCGAGGCACCGCCGATGAACGCCAGCAGGGCAAGGCTCGGGTGCGCCTCGGCCAGGGGCAGGCTGATGACGAACGAGTCGGAGATCACCGTGCCGGgcagcag                                                                                                                                                      >  2:5333501/1‑150 (MQ=255)
 cATGGTCGACAACGCCACGGCCTCGACGATCACCATGCCGGTAGCCGCCGAGGCACCGCCGATGAACGCCAGCAGGGCAAGGCTCGGGTGCGCCTCGGCCAGGGGCAGGCTGATGACGAACGAGTCGGAGAGCACCGTGCCGGgcagcag                                                                                                                                                      >  2:3594523/1‑150 (MQ=255)
    ggTCGACAACGCCACGGCCTCGACGATCACCATGCCGGTAGCCGCCGAGGCACCGCCGATGAACGCCAGCGGGGCAAGGCGCGGGTGCGCCTCGGCCAGGGGCAGGCTGATGACGAACGAGTCGGAGATCACCGTGCCGGGCAGCAGCAt                                                                                                                                                   >  1:3379406/1‑150 (MQ=255)
        gACAACGCCACGGCCTCGACGATCACCATGCCGGTAGCCGCCGAGGCACCGCCGATGAACGCCAGCAGGGCAAGGCTCGGGTGCGCCTCGGCCAGGGGCAGGCTGATGACGAACGAGTCGGAGATCACCGTGCCGGGCAGCAGCATCTGc                                                                                                                                               <  2:1898382/150‑1 (MQ=255)
            aCGCCACGGCCTCGACGATCACCATGCCGGTAGCCGCCGAGGCACCGCCGATGAACGCCAGCAGGGCAAGGCTCGGGTGCGCCTCGGCCAGGGGCAGGCTGATGACGAACGAGTCGGAGATCACCGTGCCGGGCAGCAGCATCTGCCCgg                                                                                                                                           >  1:1799340/1‑150 (MQ=255)
              gCCACGGCCTCGACGATCACCCTGCCGGTAGCCGCCGAGGCACCGCCGATGAACGCCAGCAGGGCAAGGCTCGGGTGCTCCTCGGCCAGGGGCAGGCTGATGAAGAACGAGTCGGAGATCACCGTGCCGGGCAGATGCTGCTGCCCGGca                                                                                                                                         <  2:2006604/150‑2 (MQ=255)
               ccACGGCCTCGACGATCACCATGCCGGTAGCCGCCGAGGCACCGCCGATGAACGCCAGCAGGGCAAGGCTCGGGTGCGCCTCGGCCAGGGGCAGGCTGATGACGAACGAGTCGGAGATCACCGTGCCGGGCAGCAGCATCTGCCCGGCCa                                                                                                                                        <  1:1307644/150‑1 (MQ=255)
                cACGGCCTCGACGATCACCATGCCGGTAGCCGCCGAGGCACCGCCGATGAACGCCAGCAGGGCAAGGCTCGGGTGCGCCTCGGCCAGGGGCAGGCTGATGACGAACGAGTCGGAGATCACCGTGCCGGGCAGCAGCATCTGCCCGGCCAg                                                                                                                                       <  1:832475/150‑1 (MQ=255)
                cACGGCCTCGACGATCACCATGCCGGTAGCCGCCGAGGCACCGCCGATGAACGCCAGCAGGGCAAGGCTCGGGTGCGCCTCGGCCAGGGGCAGGCTGATGACGAACGAGTCGGAGATCACCGTGCCGGGCAGCAGCATCTGCCCGGCCAg                                                                                                                                       >  2:4263061/1‑150 (MQ=255)
                 aCGGCCTCGACGATCACCATGCCGGTAGCCGCCGAGGCACCGCCGATGAACGCCAGCAGGGCAAGGCTCGGGTGCGCCTCGGCCAGGGGCAGGCTGATGACGAACGAGTCGGAGATCACCGTGCCGGGCAGCAGCATCTGCCCGGCCAgg                                                                                                                                      >  1:5356912/1‑150 (MQ=255)
                     ccTCGACGATCACCATGCCGGTAGCCGCCGAGGCACCGCCGATGAACGCCAGCAGGGCAAGGCTCGGGTGCGCCTCGGCCAGGGGCAGGCTGATGACGAACGAGTCGGAGATCACCGTGCCGGGCAGCAGCATCTGCCCGGCCAGGGCaa                                                                                                                                  >  1:2236437/1‑150 (MQ=255)
                       tCGACGATCACCATGCCGGTAGCCGCCGAGGCACCGCCGATGAACGCCAGCAGGGCAAGGCTCGGGTGCGCCTCGGCCAGGGGCAGGCTGATGACGAACGAGTCGGAGATCACCGTGCCGGGCAGCAGCATCTGCCCGGCCAGGGCAATc                                                                                                                                >  1:2049087/1‑150 (MQ=255)
                         gacgaTCACCATGCCGGTAGCCGCCGAGGCACCGCCGATGAACGCCAGCAGGGCAAGGCTCGGGTGCGCCTCGGCCAGGGGCAGGCTGATGACGAACGAGTCGGAGATCACCGTGCCGGGCAGCAGCATCTGCCCGGCCAGGGCagatgc                                                                                                                              <  2:2254723/150‑6 (MQ=255)
                              tCACCATGCCGGTAGCCGCCGAGGCACCGCCGATGAACGCCAGCAGGGCAAGGCTCGGGTGCGCCTCGGCCAGGGGCAGGCTGATGACGAACGAGTCGGAGATCACCGTGCCGGGCAGCAGCATCTGCCCGGCCAGGGCAATCGGcacca                                                                                                                         <  2:3072918/150‑1 (MQ=255)
                                  cATGCCGGTAGCCGCCGAGGCACCGCCGATGAACGCCAGCAGGGCAAGGCTCGGGTGCGCCTCGGCCAGGGGCAGGCTGATGACGAACGAGTCGGAGATCACCGTGCCGGGCAGCAGCATCTGCCCGGCCAGGGCAATCGGCACCACGaa                                                                                                                     >  2:2355188/1‑150 (MQ=255)
                                   aTGCCGGTAGCCGCCGAGGCACCGCCGATGAACGCCAGCAGGGCAAGGCTCGGGTGCGCCTCGGCCAGGGGCAGGCTGATGACGAACGAGTCGGAGATCACCGTGCCGGGCAGCAGCATCTGCCCGGCCAGGGCAATCGGCACCACGAAc                                                                                                                    >  2:2560915/1‑150 (MQ=255)
                                         ggaGCCGCCGAGGCACCGCCGATGAACGCCAGCAGGGCAAGGCTCGGGTGCGCCTCGGCCAGGGGCAGGCTGATGACGAACGAGTCGGAGATCACCGTGCCGGGCAGCAGCATCTGCCCGGCCAGGGCAATCGGCACCACGAACAGcgcg                                                                                                              <  2:4743118/148‑1 (MQ=255)
                                          tAGCCGCCGAGGCACCGCCGATGAACGCCAGCAGGGCAAGGCTCGGGTGCGCCTCGGCCAGGGGCAGGCTGATGACGAACGAGTCGGAGATCACCGTGCCGGGCAGCAGCATCTGCCCGGCCAGGGCAATCGGCACCACGAACAGCGCgg                                                                                                             <  2:4369202/150‑1 (MQ=255)
                                             ccgccgAGGCACCGCCGATGAACGCCAGCAGGGCAAGGCTCGGGTGCGCCTCGGCCAGGGGCAGGCTGATGACGAACGAGTCGGAGATCACCGTGCCGGGCAGCAGCATCTGCCCGGCCAGGGCAATCGGCACCACGAACAgc                                                                                                                 >  1:2290084/1‑143 (MQ=255)
                                             ccgccgAGGCACCGCCGATGAACGCCAGCAGGGCAAGGCTCGGGTGCGCCTCGGCCAGGGGCAGGCTGATGACGAACGAGTCGGAGATCACCGTGCCGGGCAGCAGCATCTGCCCGGCCAGGGCAATCGGCACCACGAACAgc                                                                                                                 <  2:2290084/143‑1 (MQ=255)
                                             ccgccgAGGCACCGCCGATGAACGCCAGCAGGGCAAGGCTCGGGTGCGCCTCGGCCAGGGGCAGGCTGATGACGAACGAGTCGGAGATCACCGTGCCGGGCAGCAGCATCTGCCCGGCCAGGGCAATCGGCACCACGAACAGCGCGGCCa                                                                                                          <  1:2960858/150‑1 (MQ=255)
                                              cgccgAGGCACCGCCGATGAACGCCAGCAGGGCAAGGCTCGGGTGCGCCTCGGCCAGGGGCAGGCTGATGACGAACGAGTCGGAGATCACCGTGCCGGGCAGCAGCATCTGCCCGGCCAGGGCAATCGGCACCACGAACAGCGCGGCCAg                                                                                                         >  1:3857373/1‑150 (MQ=255)
                                              cgccgAGGCACCGCCGATGAACGCCAGCAGGGCAAGGCTCGGGTGCGCCTCGGCCAGGGCCAGGCTGATGACGAACGAGTCGGAGATCACCGTGCCGGGCAGCAGCATCTGCCCGGCCAGGGCAATCGGCACCACGAACAGCGCGGCCAg                                                                                                         >  2:2554735/1‑150 (MQ=255)
                                                 cgAGGCACCGCCGATGAACGCCAGCAGGGCAAGGCTCGGGTGCGCCTCGGCCAGGGGCAGGCTGATGACGAACGAGTCGGAGATCACCGTGCCGGGCAGCAGCATCTGCCCGGCCAGGGCAATCGGCTCCACGAACAGCGCGGCCAGTGc                                                                                                      >  2:5056021/1‑150 (MQ=255)
                                                 cgAGGCACCGCCGATGAACGCCAGCAGGGCAAGGCTCGGGTGCGCCTCGGCCAGGGGCAGGCTGATGACGAACGAGTCGGAGATCACCGTGCCGGGCAGCAGCATCTGCCCGGCCAGGGCAATCGGCACCACGAACAGCGCGGCCAGTGc                                                                                                      >  1:3641060/1‑150 (MQ=255)
                                                  gAGGCACCGCCGATGAACGCCAGCAGGGCAAGGCTCGGGTGCGCCTCGGCCAGGGGCAGGCTGATGACGAACGAGTCGGAGATCACCGTGCCGGGCAGCAGCATCTGCCCGGCCAGGGCAATCGGCACCACGAACAGCGCGGCCAGTGcc                                                                                                     >  1:2334244/1‑150 (MQ=255)
                                                  gAGGCACCGCCGATGAACGCCAGCAGGGCAAGGCTCGGGTGCGCCTCGGCCAGGGGCAGGCTGAGGACGAACGAGTCGGAGATCACCGTGCCGGGCAGCAGCAGCTGCCCGGCCAGGGCAAGCGGCACCACGAACAGCGCGGCCCGTGcc                                                                                                     >  1:2321034/1‑150 (MQ=255)
                                                      cACCGCCGATGAACGCCAGCAGGGCAAGGCTCGGGTGCGCCTCGGCCAGGGTCAGGCTGATGACGAACGAGTCGGAGATCACCGTGCCGGGCAGCAGCATCTGCCCGGCCAGGGCAATCGGCACCACGAACAGCGCGGCCAGTGCCAGGt                                                                                                 <  1:2554735/150‑1 (MQ=255)
                                                      cACCGCCGATGAACGCCAGCAGGGCAAGGCTCGGGTGCGCCTCGGCCAGGGGCAGGCTGATGACGAACGAGTCGGAGATCACCGTGCCGGGCAGCAGCATCTGCCCGGCCAGGGCAATCGGCACCACGAACAGCGCGGCCAGTGCCAGGt                                                                                                 <  1:5135875/150‑1 (MQ=255)
                                                       aCCGCCGATGAACGCCAGCAGGGCAAGGCTCGGGTGCGCCTCGGCCAGGGGCAGGCTGATGACGAACGAGTCGGAGATCACCGTGCCGGGCAGCAGCATCTGCCCGGCCAGGGCAATCGGCACCACGACCAGCGCGGCCCGTGCCAGGtc                                                                                                >  1:3549554/1‑149 (MQ=255)
                                                       aCCGCCGATGAACGCCAGCAGGGCAAGGCTCGGGTGCGCCTCGGCCAGGGGCAGGCTGATGACGAACGAGTCGGAGATCACCGTGCCGGGCAGCAGCATCTGCCCGGCCAGGGCAATCGGCACCACGAACAGCGCGGCCAGTGCCAGGtc                                                                                                >  2:5048019/1‑149 (MQ=255)
                                                         cgccgATGAACGCCAGCAGGGCAAGGCTCGGGTGCGCCTCGGCCAGGGGCAGGCTGATGACGAACGAGTCGGAGATCACCGTGCCGGGCAGCAGCATCTGCCCGGCCAGGGCAATCGGCACCACGAACAGCGCGGCCAGTGCCAGGTACa                                                                                              >  2:2175771/1‑150 (MQ=255)
                                                             gATGAACGCCAGCAGGGCAAGGCTCGGGTGCGCCTCGGCCAGGGGCAGGCTGATGACGAACGAGTCGGAGATCACCGTGCCGGGCAGCAGCATCTGCCCGGCCAGGGCAATCGGCACCACGAACAGCGCGGCCAGTGCCAGGTACATCgg                                                                                          <  1:4379284/150‑1 (MQ=255)
                                                              aTGAACGCCAGCAGGGCAAGGCTCGGGTGCGCCTCGGCCAGGGGCAGGCTGATGACGAACGAGTCGGAGATCACCGTGCCGGGCAGCAGCATCTGCCCGGCCAGGGCAATCGGCACCACGAACAGCGCGGCCAGTGCCAGGTACATCggg                                                                                         <  2:2297997/150‑1 (MQ=255)
                                                              aTGAACGCCAGCAGGGCAAGGCTCGGGTGCGCCTCGGCCAGGGGCAGGCTGATGACGAACGAGTCGGAGATCACCGTGCCGGGCAGCAGCATCTGCCCGGCCAGGGCAATCGGCACCACGAACAGCGCGGCCAGTGCCAGGTACATCggg                                                                                         <  2:3050905/150‑1 (MQ=255)
                                                                gAACGCCAGCAGGGCAAGGCTCGGGTGCGCCTCGGCCAGGGGCAGGCTGATGACGAACGAGTCGGAGATCACCGTGCCGGGCAGCAGCATCTGCCCGGCCAGGGCAATCGGCACCACGAACAGCGCGGCCAGTGCCAGGTACATCGGGaa                                                                                       >  2:1064154/1‑150 (MQ=255)
                                                                 aaCGCCAGCAGGGCAAGGCTCGGGTGCGCCTCGGCCAGGGGCAGGCTGATGACGAACGAGTCGGAGATCACCGTGCCGGGCAGCAGCATCTGCCCGGCCAGGGCAATCGGCACCACGAACAGCGCGGCCAGTGCCAGGTACATCGGGAAt                                                                                      >  2:3935342/1‑150 (MQ=255)
                                                                  aCGCCAGCAGGGCAAGGCTCGGGTGCGCCTCGGCCAGGGGCAGGCTGATGACGAACGAGTCGGAGATCACCGTGCCGGGCAGCAGCATCTGCCCGGCCAGGGCAATCGGCACCACGAACAGCGCGGCCAGTGCCAGGTACATCGGGAATa                                                                                     >  1:1674883/1‑150 (MQ=255)
                                                                    gCCAGCAGGGCAAGGCTCGGGTGCGCCTCGGCCAGGGGCAGGCTGATGACGAACGAGTCGGAGATCACCGTGCCGGGCAGCAGCATCTGCCCGGCCAGGGCAATCGGCACCACGAACAGCGCGGCCAGTGCCAGGTACATCGGGAATAcc                                                                                   <  1:2306536/150‑1 (MQ=255)
                                                                        gcagGGCAAGGCGCGGGTGCGCCTCGGCCAGGGGCAGGCTGATGACGAACGAGTCGGAGATCACCGTGCCGGGCAGCAGCATCTGCCCGGCCAGGGCAATCGGCACCACGAACAGCGCGGCCAGTGCCAGGTACATCGGGAATACCCAgc                                                                               >  2:5135642/1‑150 (MQ=255)
                                                                         cagGGCAAGGCTCGGGTGCGCCTCGGCCAGGGGCAGGCTGATGACGAACGAGTCGGAGATCACCGTGCCGGGCAGCAGCATCTGCCCGGCCAGGGCAATCGGCACCACGAACAGCGCGGCCAGTGCCAGGTACATCGGGAATACCCAgca                                                                              >  1:2184637/1‑149 (MQ=255)
                                                                              cAAGGCTCGGGTGCGCCTCGGCCAGGGGCAGGCTGATGACGAACGAGTCGGAGATCACCGTGCCGGGCAGCAGCATCTGCCCGGCCAGGGCAATCGGCACCACGAACAGCGCGGCCAGTGCCAGGTACATCGGGAATACCCAGCGCGCCa                                                                         >  1:2897201/1‑150 (MQ=255)
                                                                              cAAGGCTCGGGTGCGCCTCGGCCAGGGGCAGGCTGATGACGAACGAGTCGGAGATCACCGTGCCGGGCAGCAGCATCTGCCCGGCCAGGGCAATCGGCACCACGAACAGCGCGGCCAGTGCCAGGTACATCGGGAATACCCAGCGCGCCa                                                                         <  2:3268119/150‑1 (MQ=255)
                                                                               aaGGCTCGGGTGCGCCTCGGCCAGGGGCAGGCTGATGACGAACGAGTCGGAGATCACCGTGCCGGGCAGCAGCATCTGCCCGGCCAGGGCAATCGGCACCACGAACAGCGCGGCCAGTGCCAGGTACATCGGGAATACCCAGCGCGCCAg                                                                        >  2:5400027/1‑150 (MQ=255)
                                                                                aGGCTCGGGTGCGCCTCGGCCAGGGGCAGGCTGATGACGAACGAGTCGGAGATCACCGTGCCGGGCAGCAGCATCTGCCCGGCCAGGGCAATCGGCTCCACGAACAGCGCGGCCAGTGCCAGGTACATCGGGAATACCCAGCGCGCCAgg                                                                       <  1:5056021/150‑1 (MQ=255)
                                                                                       ggTGCGCCTCGGCCAGGGGCAGGCTGATGACGAACGAGTCGGAGATCACCGTGCCGGGCAGCAGCATCTGCCCGGCCAGGGCAATCGGCACCACGAACAGCGCGGCCAGTGCCAGGTACATCg                                                                                           <  2:1119639/123‑1 (MQ=255)
                                                                                       ggTGCGCCTCGGCCAGGGGCAGGCTGATGACGAACGAGTCGGAGATCACCGTGCCGGGCAGCAGCATCTGCCCGGCCAGGGCAATCGGCACCACGAACAGCGCGGCCAGTGCCAGGTACATCGGGAATACCCAGCGCGCCAGGCGCAAGt                                                                >  2:5331638/1‑150 (MQ=255)
                                                                                        gTGCGCCTCGGCCAGGGGCAGGCTGATGACGAACGAGTCGGAGATCACCGTGCCGGGCAGCAGCATCTGCCCGGCCAGGGCAATCGGCACCACGAACAGCGCGGCCAGTGCCAGGTACATCGGGAATACCCAGCGCGCCAGGCGCAAGTc                                                               <  2:1239770/150‑1 (MQ=255)
                                                                                        gTGCGCCTCGGCCAGGGGCAGGCTGATGACGAACGAGTCGGAGATCACCGTGCCGGGCAGCAGCATCTGCCCGGCCAGGGCAATCGGCACCACGAACAGCGCGGCCAGTGCCAGGTACATCGGGAATACCCAGCGCGCCAGGCGCAAGTc                                                               <  2:4196016/150‑1 (MQ=255)
                                                                                        gTGCGCCTCGGCCAGGGGCAGGCTGATGACGAACGAGTCGGAGATCACCGTGCCGGGCAGCAGCATCTGCCCGGCCAGGGCAATCGGCACCACGAACAGCGCGGCCAGTGCCAGGTACATCGGGAATACCCAGCGCGCCAGGCGCAAGTc                                                               <  2:515076/150‑1 (MQ=255)
                                                                                          gcgcCTCGGCCAGGGGCAGGCTGATGACGAACGAGTCGGAGATCACCGTGCCGGGCAGCAGCATCTGCCCGGCCAGGGCAATCGGCACCACGAACAGCGCGGCCAGTGCCAGGTACATCGGGAATACCCAGCGCGCCAGGCGCAAGTCCt                                                             >  2:1573864/1‑150 (MQ=255)
                                                                                            gcCTCGGCCAGGGGCAGGCTGATGACGAACGAGTCGGAGATCTGCGTGCCGGTGAGCAGCATCTGCCCGGCCAGGGCAATCGGCACCACGAACAGCGCGGCCAGTGCCAGGTACATCGGGAATACCCAGCGCGCCAGGCGCAAGTCCTGc                                                           <  2:3468826/150‑1 (MQ=255)
                                                                                            gcCTCGGCCAGGGGCAGGCTGATGACGAACGAGTCGGAGATCACCGTGCCGGGCAGCAGCATCTGCCCGGCCAGGGCAATCGGCACCACGAACAGCGCGGCCAGTGCCAGGTACATCGGGAATACCCAGCGCGCCAGGCGCAAGTCCTGc                                                           >  2:2085137/1‑150 (MQ=255)
                                                                                            gcCTCGGCCAGGGGCAGGCTGATGACGAACGAGTCGGAGATCACCGTGCCGGGCAGCAGCATCTGCCCGGCCAGGGCAATCGGCACCACGAACAGCGCGGCCAGTGCCAGGTACATCGGGAATACCCAGCGCGCCAGGCGCAAGTCCTGc                                                           >  2:4606836/1‑150 (MQ=255)
                                                                                            gcCTCGGCCAGGGGCAGGCTGATGACGAACGAGTCGGAGATCACCGTGCCGGGCAGCAGCATCTGCCCGGCCAGGGCAATCGGCACCACGAACAGCGCGGCCAGTGCCAGGTACATCGGGAATACCCAGCGCGCCAGGCGCAAGTCCTGc                                                           <  2:2802974/150‑1 (MQ=255)
                                                                                              cTCGGCCAGGGGCAGGCTGATGACGAACGAGTCGGAGATCACCGTGCCGGGCAGCAGCATCTGCCCGGCCAGGGCAATCGGCACCACGAACAGCGCGGCCAGTGCCAGGTACATCGGGAATACCCAGCGCGCCAGGCGCAAGTCCTGCgg                                                         >  2:721411/1‑150 (MQ=255)
                                                                                              cTCGGCCAGGGGCAGGCTGATGACGAACGAGTCGGAGATCACCGTGCCGGGCAGCAGCATCTGCCCGGCCAGGGCAACCGGCACCACGAACAGCGCGGCCAGTGCCAGGTACATCGGGACTACCCAGCGCGCCAGGCGCAAGTCCTGCgg                                                         >  1:3671391/1‑150 (MQ=255)
                                                                                              cTCGGCCAGGGGCAGGCTGATGACGAACGAGTCGGAGATCACCGTGCCGGGCAGCAGCATCTGCCCGGCCAGGGCAACCGGCACCACGAACAGCGCGGCCAGTGCCAGGTACATCGGGAATACCCAGCGCGCCAGGCGCAAGTCCTGCgg                                                         >  2:4960416/1‑150 (MQ=255)
                                                                                                   ccAGGGGCAGGCTGATGACGAACGAGTCGGAGATCACCGTGCCGGGCAGCAGCATCTGCCCGGCCAGGGCAATCGGCACCACGAACAGCGCGGCCAGTGCCAGGTACATCGGGAATACCCAGCGCGCCAGGCGCAAGTCCTGCGGTTCGa                                                    <  2:1272130/150‑1 (MQ=255)
                                                                                                    cAGGGGCAGGCTGATGACGAACGAGTCGGAGATCACCGTGCCGGGCAGCAGCATCTGCCCGGCCAGGGCAATCGGCACCACGAACAGCGCGGCCAGTGCCAGGTACATCGGGAATACCCAGCGCGCCAGGCGCAAGTCCTg                                                            >  1:4890287/1‑141 (MQ=255)
                                                                                                    cAGGGGCAGGCTGATGACGAACGAGTCGGAGATCACCGTGCCGGGCAGCAGCATCTGCCCGGCCAGGGCAATCGGCACCACGAACAGCGCGGCCAGTGCCAGGTACATCGGGAATACCCAGCGCGCCAGGCGCAAGTCCTGCGGTTCGAt                                                   >  1:2911112/1‑150 (MQ=255)
                                                                                                          cAGGCTGATGACGAACGAGTCGGAGATCACCGTGCCGGGCAGCAGCATCTGCCCGGCCAGGGCAATCGGCACCACGAACAGCGCGGCCAGTGCCAGGTACATCGGGAATACCCAGCGCGCCAGGCGCAAGTCCTGCGGTTCGATGTtctc                                             <  1:611565/150‑1 (MQ=255)
                                                                                                               tgatgaCGAACGAGTCGGAGATCACCGTGCCGGGCAGCAGCATCTGCCCGGCCAGGGCAATCGGCACCACGAACAGCGCGGCCAGTGCCAGGTACATCGGGAATACCCAGCGCGCCAGGCGCAAGTCCTGCGGTTCGATGTTCTccacca                                        >  1:4609437/1‑150 (MQ=255)
                                                                                                                gatgaCGAACGAGTCGGAGATCACCGTGCCGGGCAGCAGCATCTGCCCGGCCAGGGCAATCGGCACCACGAACAGCGCGGCCAGTGCCAGGTACATCGGGAATACCCAGCGCGCCAGGCGCAAGTCCTGCGGTTCGATGTTCTCcaccac                                       <  1:1019484/150‑1 (MQ=255)
                                                                                                                gatgaCGAACGAGTCGGAGATCACCGTGCCGGGCAGCAGCATCTGCCCGGCCAGGGCAATCGGCACCACGAACAGCGCGGCCAGTGCCAGGTACATCGGGAATACCCAGCGCGCCAGGCGCAAGTCCTGCGGTTCGATGTTCTCcaccac                                       >  1:5304829/1‑150 (MQ=255)
                                                                                                                  tgaCGAACGAGTCGGAGATCACCGTGCCGGGCAGCAGCATCTGCCCGGCCAGGGCAATCGGCACCACGAACAGCGCGGCCAGTGCCAGGTACATCGGGAATACCCAGCGCGCCAGGCGCAAGTCCTGCGGTTCGATGTTCTCCACCACgg                                     >  1:3784381/1‑150 (MQ=255)
                                                                                                                  tgaCGAACGAGTCGGAGATCACCGTGCCGGGCAGCAGCATCTGCCCGGCCAGGGCAATCGGCACCACGAACAGCGCGGCCAGTGCCAGGTACATCGGGAATACCCAGCGCGCCAGGCGCAAGTCCTGCGGTTCGATGTTCTCCACCACgg                                     >  1:150586/1‑150 (MQ=255)
                                                                                                                   gaCGAACGAGTCGGAGATCACCGTGCCGGGCAGCAGCATCTGCCCGGCCAGGGCAATCGGCACCACGAACAGCGCGGCCAGTGCCAGGTACATCGGGAATACCCAGCGCGCCAGGCGCAAGTCCTGCGGTTCGATGTTCTCCACCACGGt                                    <  1:4944006/150‑1 (MQ=255)
                                                                                                                        acgaGTCGGAGATCACCGTGCCGGGCAGCAGCATCTGCCCGGCCAGGGCAATCGGCACCACGAACAGCGCGGCCAGTGCCAGGTACATCGGGAATACCCAGCGCGCCAGGCGCAAGTCCTGCGGTTCGATGTTCTCCACCACGGTAACGt                               >  1:5117127/1‑150 (MQ=255)
                                                                                                                         cgaGTCGGAGATCACCGTGCCGGGCAGCAGCATCTGCCCGGCCAGGGCAATCGGCACCACGAACAGCGCGGCCAGTGCCAGGTACATCGGGAATACCCAGCGCGCCAGGCGCAAGTCCTGCGGTTCGATGTTCTCCACCACGGTAACGTg                              <  1:2423960/150‑1 (MQ=255)
                                                                                                                            gTCGGAGATCACCGTGCCGGGCAGCAGCATCTGCCCGGCCAGGGCAATCGGCACCACGAACAGCGCGGCCAGTGCCAGGTACATCGGGAATACCCAGCGCGCCAGGCGCAAGTCCTGCGGTTCGATGTTCTCCACCACGGTAACGTGGaa                           <  2:4161326/150‑1 (MQ=255)
                                                                                                                             tCGGAGATCACCGTGCCGGGCAGCAGCATCTGCCCGGCCAGGGCAATCGGCACCACGAACAGCGCGGCCAGTGCCAGGTACATCGGGAATACCCAGCGCGCCAGGCGCAAGTCCTGCGGTTCGATGTTCTCCACCACGGTAACGTGGAAc                          >  1:1495821/1‑150 (MQ=255)
                                                                                                                               ggAGATCACCGTGCCGGGCAGCAGCATCTGCCCGGCCAGGGCAATCGGCACCACGAACAGCGCGGCCAGTGCCAGGTACATCGGGAATACCCAGCGCGCCAGGCGCAAGTCCTGCGGTTCGATGTTCTCCACCACGGTCACGTGGAACTg                        >  1:927569/1‑150 (MQ=255)
                                                                                                                                gagaTCACCGTGCCGGGCAGCAGCATCTGCCCGGCCAGGGCAATCGGCACCACGAACAGCGCGGCCAGTGCCAGGTACATCGGGAATACCCAGCGCGCCAGGCGCAAGTCCTGCGGTTCGATGTTCTCCACCACGTTACCGTggtggaga                       <  2:1087913/150‑7 (MQ=255)
                                                                                                                                gagaTCACCGTGCCGGGCAGCAGCATCTGCCCGGCCAGGGCAATCGGCACCACGAACAGCGCGGCCAGTGCCAGGTACATCGGGAATACCCAGCGCGCCAGGCGCAAGTCCTGCGGTTCGATGTTCTCCACCACGGTAACGTGGAACTGt                       >  1:805433/1‑150 (MQ=255)
                                                                                                                                gagaTCACCGTGCCGGGCAGCAGCATCTGCCCGGCCAGGGCAATCGGCACCACGAACAGCGCGGCCAGTGCCAGGTACATCGGGAATACCCAGCGCGCCAGGCGCAAGTCCTGCGGTTCGATGTTCTCCACCACGGTAACGTGGAACTGt                       >  1:3927804/1‑150 (MQ=255)
                                                                                                                                  gaTCACCGTGCCGGGCAGCAGCATCTGCCCGGCCAGGGCAATCGGCACCACGAACAGCGCGGCCAGTGCCAGGTACATCGGGAATACCCAGCGCGCCAGGCGCAAGTCCTGCGGTTCGATGTTCTCCACCACGGTAACGTGGAACTGTCg                     <  1:4708174/150‑1 (MQ=255)
                                                                                                                                  gaTCACCGTGCCGGGCAGCAGCATCTGCCCGGCCAGGGCAATCGGCACCACGAACAGCGCGGCCAGTGCCAGGTACATCGGGAATACCCAGCGCGCCAGGCGCAAGTCCTGCGGTTCGATGTTCTCCACCACGGTAACGTGGAACTGTCg                     >  2:2486299/1‑150 (MQ=255)
                                                                                                                                  gaTCACCGTGCCGGGCAGCAGCATCTGCCCGGCCAGGGCAATCGGCACCACGAACAGCGCGGCCAGTGCCAGGTACATCGGGAATACCCAGCGCGCCAGGCGCAAGTCCTGCGGTTCGATGTTCTCCACCACGGTAACGTGGAACTGTCg                     <  1:2028489/150‑1 (MQ=255)
                                                                                                                                  gaTCACCGTGCCGGGCAGCAGCATCTGCCCGGCCAGGGCAATCGGCACCACGAACAGCGCGGCCAGTGCCAGGTACATCGGGAATACCCAGCGCGCCAGGCGCAAGTCCTGCGGTTCGATGTTCTCCACCACGGTAACGTGGAACTGTCg                     >  2:2731920/1‑150 (MQ=255)
                                                                                                                                      aCCGTGCCGGGCAGCAGCATCTGCCCGGCCAGGGCAATCGGCACCACGAACAGCGCGGCCAGTGCCAGGTACATCGGGAATACCCAGCGCGCCAGGCGCAAGTCCTGCGGTTCGATGTTCTCCACCACGGTAACGTGGAACTGTCGGggc                 >  2:5336339/1‑150 (MQ=255)
                                                                                                                                       ccGTGCCGGGCAGCAGCATCTGCCCGGCCAGGGCAATCGGCACCACGAACAGCGCGGCCAGTGCCAGGTACATCGGGAATACCCAGCGCGCCAGGCGCAAGTCCTGCGGTTCGATGTTCTCCACCACGGTAACGTGGAACTGTCGGggca                >  1:5386709/1‑150 (MQ=255)
                                                                                                                                       ccGTGCCGGGCAGCAGCATCTGCCCGGCCAGGGCAATCGGCACCACGAACAGCGCGGCCAGTGCCAGGTACATCGGGAATACCCAGCGCGCCAGGCGCAAGTCCTGCGGTTCGATGTTCTCCACCACGGTAACGTGGAACTGTCGGggca                <  2:2423091/150‑1 (MQ=255)
                                                                                                                                       ccGTGCCGGGCAGCAGCATCTGCCCGGCCAGGGCAATCGGCACCACGAACAGCGCGGCCAGTGCCAGGTACATCGGGAATACCCAGCGCGCCAGGCGCAAGTCCTGCGGTTCGATGTTCTCCACCACGGTAACGTGGAACTGTCGGggca                <  1:3594523/150‑1 (MQ=255)
                                                                                                                                       ccGTGCCGGGCAGCAGCATCTGCCCGGCCAGGGCAATCGGCACCACGAACAGCGCGGCCAGTGCCAGGTACATCGGGAATACCCAGCGCGCCAGGCGCAAGTCCTGCGGTTCGATGTTCTCCACCACGGTAACGTGGAACTGTCGGggca                <  1:1122887/150‑1 (MQ=255)
                                                                                                                                       ccGTGCCGGGCAGCAGCATCTGCCCGGCCAGGGCAATCGGCACCACGAACAGCGCGGCCAGTGCCAGGTACATCGGGAATACCCAGCGCGCCAGGCGCAAGTCCTGCGGTTCGATGTTCTCCACCACGGTAACGTGGAACTGTCGGggca                >  1:2662542/1‑150 (MQ=255)
                                                                                                                                         tggCCGGGCAGATGCTGCTGCCCGGCCAGGGCAATCGGCACCACGAACAGCGCGGCCAGTGCCAGGTACATCGGGAATACCCAGCGCGCCAGGCGCAAGTCCTGCGGTTCGATGTTCTCCACCACGGTAACGTGGAACTGTCGGggcagg              >  2:445707/3‑150 (MQ=255)
                                                                                                                                         gTGCCGGGCAGCAGCATCTGCCCGGCCAGGGCAATCGGCACCACGAACAGCGCGGCCAGTGCCAGGTACATCGGGAATACCCAGCGCGCCAGGCGCAAGTCCTGCGGTTCGATGTTCTCCACCACGGTAACGTGGAACTGTCGGggcagg              >  2:4829012/1‑150 (MQ=255)
                                                                                                                                          ggCCGGGCAGATGCTGCTGCCCGGCCAGGGCAATCGGCACCACGAACAGCGCGGCCAGTGCCAGGTACATCGGGAATACCCAGCGCGCCAGGCGCAAGTCCTGCGGTTCGATGTTCTCCACCACGGTAACGTGGAACTGTCGGggcaggc             >  1:4910832/2‑150 (MQ=255)
                                                                                                                                            ccGGGCAGCAGCATCTGCCCGGCCAGGGCAATCGGCACCACGAACAGCGCGGCCAGTGCCAGGTACATCGGGAATACCCAGCGCGCCAGGCGCAAGTCCTGCGGTTCGATGTTCTCCACCACGGTAACGTGGAACTGTCGGGgcaggcag           >  1:3319795/1‑150 (MQ=255)
                                                                                                                                             cGGGCAGATGCTGCTGCCCGGCCAGGGCAATCGGCACCACGAACAGCGCGGCCAGTGCCAGGTACATCGGGAATACCCAGCGCGCCAGGCGCAAGTCCTGCGGTTCGATGTtctc                                             <  2:2251829/115‑1 (MQ=255)
                                                                                                                                             cGGGCAGATGCTGCTGCCCGGCCAGGGCAATCGGCACCACGAACAGCGCGGCCAGTGCCAGGTACATCGGGAATACCCAGCGCGCCAGGCGCAAGTCCTGCGGTTCGATGTTCTCCACCACGGTAACGTGGAACTGTCGGGGCAGGCAga          >  1:1280753/1‑150 (MQ=255)
                                                                                                                                              gggCAGCAGCATCTGCCCGGCCAGGGCAATCGGCACCACGAACAGCGCGGCCAGTGCCAGGTACATCGGGAATACCCAGCGCGCCAGGCGCAAGTCCTGCGGTTCGATGTTCTCCACCACGGTAACGTGGAACTGTCGGGGCAGGCAgat         <  1:202129/150‑1 (MQ=255)
                                                                                                                                              gggCAGCAGCATCTGCCCGGCCAGGGCAATCGGCACCACGAACAGCGCGGCCAGTGCCAGGTACATCGGGAATACCCAGCGCGCCAGGCGCAAGTCCTGCGGTTCGATGTTCTCCACCACGGTAACGTGGAACTGTCGGGGCAGGCAgat         <  2:2906116/150‑1 (MQ=255)
                                                                                                                                                  agcagcaTCTGCCCGGCCAGGGCAATCGGCACCACGAACAGCGCGGCCAGTGCCAGGTACATCGGGAATACCCAGCGCGCCAGGCGCAAGTCCTGCGGTTCGATGTTCTCCACCACGGTAACGTGGAACTGTCGGGGCAGGCAGATGAtt     <  1:1851785/150‑1 (MQ=255)
                                                                                                                                                    cagcaTCTGCCCGGCCAGGGCAATCGGCACCACGAACAGCGCGGCCAGTGCCAGGTACATCGGGAATACCCAGCGCGCCAGGCGCAAGTCCTGCGGTTCGATGTTCTCCACCACGGTAACGTGGAACTGTCGGGGCAGGCAGATGATTGc   <  1:3096331/150‑1 (MQ=255)
                                                                                                                                                    cagcaTCTGCCCGGCCAGGGCAATCGGCACCACGAACAGCGCGGCCAGTGCCAGGTACATCGGGAATACCCAGCGCGCCAGGCGCAAGTCCTGCGGTTCGATGTTCTCCACCACGGTAACGTGGAACTGTCGGGGCAGGCAGATGATTGc   <  1:4263061/150‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
CCATGGTCGACAACGCCACGGCCTCGACGATCACCATGCCGGTAGCCGCCGAGGCACCGCCGATGAACGCCAGCAGGGCAAGGCTCGGGTGCGCCTCGGCCAGGGGCAGGCTGATGACGAACGAGTCGGAGATCACCGTGCCGGGCAGCAGCATCTGCCCGGCCAGGGCAATCGGCACCACGAACAGCGCGGCCAGTGCCAGGTACATCGGGAATACCCAGCGCGCCAGGCGCAAGTCCTGCGGTTCGATGTTCTCCACCACGGTAACGTGGAACTGTCGGGGCAGGCAGATGATTGCC  >  NC_002947/1889252‑1889550

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑