BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	2,104,964	C→G	5.1%	noncoding (35/76 nt)	PP_t23 →	tRNA‑Gly

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	2,104,964	0	C	G	5.1%	499.6 / 11.7	155	noncoding (35/76 nt)	PP_t23	tRNA‑Gly
Reads supporting (aligned to +/- strand): ref base C (66/81); new base G (3/5); total (69/86)
Fisher's exact test for biased strand distribution p-value = 7.33e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.89e-01

Only 100 of 159 total aligned reads displayed.

GAGGTTCATGTTCTGGTGCACTGAAAAGTGTTGTGTGTCCCCTTCGTCTAGTGGCCTAGGACACCGCCCTTTCACGGCGGTAACAGGGGTTCGAGTCCCCTAGGGGACGCCAAATGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAGTTTAAATACTGTGGCGTCTATGCGGTGCAGTGGTGGTGAAGGTCGTAAAGGCGCTTCACGCCGAACGTGGTAAAGCTTCAAATTGCGGGAATAGCTCAGTTGGTAGA  >  NC_002947/2104815‑2105113

gAGGTTCATGTTCTGGTGCACTGAAAAGTGTTGTGTGTCCCCTTCGTCTAGTGGCCTAGGACACCGCCCTTTCACGGCGGTAACAGGGGTTCGAGTCCCCTAGGGGACGCCAAATGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGc                                                                                                                                                       <  1:4440881/150‑1 (MQ=255)

        tGTTCTGGTGCACTGAAAAGTGTTGTGTGTCCCCTTCGTCTAGTGGCCTAGGACACCGTCCTTTCACGGCGGTAACAGGGGTTCGAGTCCCCTAGGGGACGCCAAATGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCg                                                                                                                                               >  1:2212768/1‑150 (MQ=255)

        tGTTCTGGTGCACTGAAAAGTGTTGTGTGTCCCCTTCGTCTAGTGGCCTAGGACACCGCCCTTTCACGGCGGTAACAGGGGTTCGAGTCCCCTAGGGGACGCCAAATGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGGCAAGGTCg                                                                                                                                               <  2:5201426/150‑1 (MQ=255)

        tGTTCTGGTGCACTGAAAAGTGTTGTGTGTCCCCTTCGTCTAGTGGCCTAGGACACCGCCCTTTCACGGCGGTAACAGGGGTTCGAGTCCCCTAGGGGACGCCAAATGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCg                                                                                                                                               >  2:2439159/1‑150 (MQ=255)

         gTTCTGGTGCACTGAAAAGTGTTGTGTGTCCCCTTCGTCTAGTGGCCTAGGACACCGCCCTTTCACGGCGGTAACAGGGGTTCGAGTCCCCTAGGGGACGCCAAATGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCgg                                                                                                                                              >  2:1836283/1‑150 (MQ=255)

         gTTCTGGTGCACTGAAAAGTGTTGTGTGTCCCCTTCGTCTAGTGGCCTAGGACACCGCCCTTTCACGGCAGTAACAGGGGTTCGAGTCCCCTAGGGGACGCCAAATGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCgg                                                                                                                                              <  2:4247282/150‑1 (MQ=255)

          ttCTGGTGCACTGAAAAGTGTTGTGTGTCCCCTTCGTCTAGTGGCCTAGGACACCGCCCTTTCACGGCGGTAACAGGGGTTCGAGTCCCCTAGGGGACGCCAAATGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCggg                                                                                                                                             <  2:172023/150‑1 (MQ=255)

          ttCTGGTGCACTGAAAAGTGTTGTGTGTCCCCTTCGTCTAGTGGCCTAGGACACCGCCCTTTCACGGCGGTAACAGGGGTTCGAGTCCCCTAGGGGACGCCAAATGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCggg                                                                                                                                             <  2:2303584/150‑1 (MQ=255)

          ttCTGGTGCACTGAAAACAGCTGTGTGTCACCTTCTTCTCCTCCCCTTGGACACCCCCCACTCACTTCGGTAACATGGGGTCGTGTGCCCTATGGGTCGCCAAATGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCggg                                                                                                                                             <  2:1783546/150‑1 (MQ=9)

           tCTGGTGCACTGAAAAGTGTTGTGTGTCCCCTTCGTCTCGTGGCCTAGGACACCGCCCTTTCACGGCGGTAACTGGGGTTCGAGTCCCCGAGGGGACGCCAAATGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGGCAAGGTCgtgc                                                                                                                                            <  2:1134203/150‑4 (MQ=17)

                     tGAAAAGTGTTGTGTGTCCCCTTCGTCTAGTGGCCTAGGACACCGCCCTTTCACGGCGGTAACAGGGGTTCGAGTCCCCTAGGGGACGCCAAATGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTc                                                                                                                                  >  2:1468442/1‑150 (MQ=255)

                       aaaaGTGTTGTGTGTCCCCTTCGTCTAGTGGCCTAGGACACCGCCCTTTCACGGCGGTAACAGGGGTTCGAGTCCCCTAGGGGACGCCAAATGCGGGAATAGCTCAGTTTGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGa                                                                                                                                <  1:5428219/150‑1 (MQ=255)

                       aaaaGTGTTGTGTGTCCCCTTCGTCTAGTGGCCTAGGACACCGCCCTTTCACGGCGGTAACAGGGGTTCGAGTCCCCTAGGGGACGCCAAATGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGa                                                                                                                                <  2:1988277/150‑1 (MQ=255)

                       aaaaGTGTTGTGTGTCCCCTTCGTCTAGTGGCCTAGGACACCGCCCTTTCACGGCGGTAACAGGGGTTCGAGTCCCCTAGGGGACGCCAAATGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGa                                                                                                                                >  1:2789355/1‑150 (MQ=255)

                                  gtgtCCCCTTCGTCTAGTGGCCTAGGACACCGCCCTTTCACGGCGGTAACAGGGGTTCGAGTCCCCTAGGGGACGCCAAATGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTcc                                                                                                                     <  1:3752936/150‑1 (MQ=34)

                                  gtgtCCCCTTCCTCTAGTGGCCGAGGACACCGCCCCTTCAGTTCGGTAACAGGGGTTCGCGTCCCCTATGGGAAGGCAAATGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTcc                                                                                                                     <  2:4854960/150‑1 (MQ=11)

                                   tgtCCCCTTCGTCTAGTGGCCTAGGACACCGCCCTTTCACGGCGGTAACAGGGGTTCGAGTCCCCGAGGGGACGCCAAATGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTccc                                                                                                                    >  1:1867023/1‑150 (MQ=21)

                                     tCCCCTTCGTCTAGTGGCCTAGGACACCGCCCTTTCACGGCGGTAACAGGGGTTCGAGTCCCCTAGGGGACGCCAAATGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGGCTCGTTTCCCGc                                                                                                                  >  1:1744490/1‑150 (MQ=21)

                                                tAGTGGCCTAGGACACCGCCCTTTCACGGCGGTAACAGGGGTTCGAGTCCCCTAGGGGACGCCAAATGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAGttt                                                                                                          <  2:4318044/147‑1 (MQ=34)

                                                      ccTAGGACACCGCCCTTTCACGGCGGTAACAGGGGTTCGAGTCCCCTAGGGGACGCCAAATGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAGTTTAAATACtgt                                                                                                 <  2:3392045/150‑1 (MQ=255)

                                                        tAGGACACCGCCCTTTCACGGCGGTAACAGGGGTTCGAGTCCCCTAGGGGACGCCAAATGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAGTTTAAATACTGTgg                                                                                               >  2:4480902/1‑150 (MQ=255)

                                                         ctgtcacCGCCCTTTCACGGCGGTAACAGGGGTTCGAGTCCCCTAGGGGACGCCAAATGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAGTTTAATTACTGAGGc                                                                                              >  1:1219008/5‑150 (MQ=255)

                                                         aGGACACCGCCCTTTCACGGCGGTAACAGGGGTTCGAGTCCCCTAGGGGACGCCAAATGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAGTTTAAATACTGTGGc                                                                                              >  1:583634/1‑150 (MQ=255)

                                                           gACACCGCCCTTTCACGGCGGTAACAGGGGTTCGAGTCCCCTAGGGGACGCCAAATGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAGTTTAAATACTGTGGCGt                                                                                            <  1:1467830/150‑1 (MQ=255)

                                                              acCGCCCTTTCACGGCGGTAACAGGGGTTCGAGTCCCCTAGGGGACGCCAAATGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAGTTTAAATACTGTGGCGTCTa                                                                                         >  1:4849531/1‑150 (MQ=255)

                                                              acCGCCCTTTCACGGCGGTAACAGGGGTTCGAGTCCCCTAGGGGACGCCAAATGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAGTTTAAATACTGTGGCGTCTa                                                                                         >  2:517158/1‑150 (MQ=255)

                                                                 gCCCTTTCACGGCGGTAACAGGGGTTCGAGTCCCCTAGGGGACGCCAAATGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAGTTTAAATACTGTGGCGTCTATGc                                                                                      >  1:3482471/1‑150 (MQ=255)

                                                                 gCCCTTTCACGGCGGTAACAGGGGTTCGAGTCCCCTAGGGGACGCCAAATGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAGTTTAAATACTGTGGCGTCTATGc                                                                                      <  2:2110722/150‑1 (MQ=255)

                                                                  cccTTTCACGGCGGTAACAGGGGTTCGAGTCCCCTAGGGGACGCCAAATGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAGTTTAAATACTGTGGCGTCTATGCg                                                                                     >  2:3400010/1‑150 (MQ=255)

                                                                     tttCACGGCGGTAACAGGGGTTCGAGTCCCCTAGGGGACGCCAAATGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAGTTTAAATACTGTGGCGTCTATGCGGTg                                                                                  >  1:824982/1‑150 (MQ=255)

                                                                     tttCACGGCGGTAACAGGGGTTCGAGTCCCCTAGGGGACGCCAAATGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAGATTAAATACTGTGGCGTCTATGAGGTg                                                                                  >  1:3005230/1‑150 (MQ=255)

                                                                         aCGGCGGTAACAGGGGTTCGAGTCCCCTAGGGGACGCCAAATGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAGTTTAAATAATGTGGCGTCTATGCGGGGCAgt                                                                              >  2:5104401/1‑150 (MQ=255)

                                                                          gggcggTAACAGGGGTTCGAGTCCCCTAGGGGACGCCAAATGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAGTTTAAATACTGTGGCGTCTATGCGGTGCAgtg                                                                             <  2:5072319/149‑1 (MQ=255)

                                                                           ggcggTAACAGGGGTTCGAGTCCCCTAGGGGTCGCCATATGCGGGAAGAGCTCCGTTGGTAGAGCACGACCTTGCCATGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAGTTTAAATACTGTGGCGTCTATGCGGTGCAgtgg                                                                            <  1:5188963/150‑1 (MQ=17)

                                                                           ggcggTAACAGGGGTTCGAGTCCCCTAGGGGACGCCAAATGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAGTTTAAATACTGTGGCGTCTATGCGGTGCAgtgg                                                                            <  1:4631817/150‑1 (MQ=255)

                                                                            gcggTAACAGGGGTTCGAGTCCCCTAGGGGACTCCAAATGCGGGAATAGCTCAGTTTGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAGTTTAAATACTGTGGCGTCTATGCGGTGCAgtggt                                                                           <  1:495898/150‑1 (MQ=255)

                                                                             cggTAACAGGGGTTCGAGTCCCCTAGGGGACGCCAAATGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAGTTTAAATACTGTGGCGTCTATGCGGTGCAgtggtg                                                                          <  2:3983585/150‑1 (MQ=255)

                                                                               gTAACAGGGGTTCGAGTCCCCTAGGGTACGCCAAATGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAGTTTAAATACTGTGGCGTCTATGCGGTGCAgtggtggt                                                                        <  1:1290329/150‑1 (MQ=255)

                                                                               gTAACAGGGGTTCGAGTCCCCTAGGGGACGCCAAATGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAGTTTAAATACTGTGGCGTCTATGCGGTGCAgtggtggt                                                                        >  2:613923/1‑150 (MQ=255)

                                                                                   cAGGGGTTCGAGTCCCCTAGGGGACGCCAAATGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAGTTTAAATACTGTGGCGTCTATGCGGTGCAGTGGTGGTGAAg                                                                    <  2:824982/150‑1 (MQ=255)

                                                                                   cAGGGGTTCGAGTCCCCTAGGGGACGCCAAATGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAGTTTAAATACTGTGGCGTCTATGCGGTGCAGTGGTGGTGAAg                                                                    <  1:136472/150‑1 (MQ=255)

                                                                                    aGGGGTTCGAGTCCCCTAGGGGACGCCAAATGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAGTTTAAATACTGTGGCGTCTATGCGGTGCAGTGGTGGTGAAgg                                                                   >  2:2771737/1‑150 (MQ=255)

                                                                                    aGGGGTTCGAGTCCCCTAGGGGACGCCAAATGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAGTTTAAATACTGTGGCGTCTATGCGGTGCAGTGGTGGTGAAgg                                                                   >  1:5033719/1‑150 (MQ=255)

                                                                                    aGGGGTTCGAGTCCCCTAGGGGACGCCAAATGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAGTTTAAATACTGTGGCGTCTATGCGGTGCAGTGGTGGTGAAgg                                                                   >  2:1454799/1‑150 (MQ=255)

                                                                                     ggggTTCGAGTCCCCTAGGGGACGCCAAATGCGGGAAGAGCCCAGTATGGAGCGCACGACGTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAGTTTAAATACTGTGGCGTCTATGCGGTGCAGTGGTGGTGAAGGt                                                                  <  2:2554009/150‑1 (MQ=21)

                                                                                       ggTTCGAGTCCCCTAGGGGACGCCAAATGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAGTTTAAATACTGTGGCGTCTATGCGGTGCAGTGGTGGTGAAGGTCg                                                                <  2:2863031/150‑1 (MQ=255)

                                                                                        gTTCGAGTCCCCTAGGGGACGCC‑AATGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAGTTTAAATACTGTGGCGTCTATGCGGt                                                                                   >  1:3356742/1‑129 (MQ=255)

                                                                                               tCCCCTAGGGGACGCCAAATGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGa                                                                                                                                      >  1:2476886/1‑72 (MQ=39)

                                                                                               tCCCCTAGGGGACGCCAAATGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGa                                                                                                                                      <  2:2476886/72‑1 (MQ=39)

                                                                                                ccccTAGGGGACGCCAAATGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAGTTTAAATACTGTGGCGTCTATGCGGTGCAGTGGTGGTGAAGGTCGTAAAGgcgc                                                       <  1:1468442/150‑1 (MQ=255)

                                                                                                ccccTAGGGGACGCCAAATGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAGTTTAAATACTGTGGCGTCTATGCGGTGCAGTGGTGGTGAAGGTCGTAAAGgcgc                                                       <  2:2283037/150‑1 (MQ=255)

                                                                                                ccccTAGGGGACGCCAAATGCGGGAATAGCTCAGTTGGTAGAGCACGACCGTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAGTTTAAATACTGTGGCGTCTATGCGGTGCAGTGGTGGTGAAGGTCGTAAAGgcgc                                                       <  2:742058/150‑1 (MQ=255)

                                                                                                 cccTAGGGGACGCCAAATGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAGTTTAATAACTGTGGCGTATATGCGGTGAAGTTGTGGTGAAGGTCGTAAAGGCGCt                                                      >  2:2562461/1‑150 (MQ=18)

                                                                                                 cccTAGGGGACGCCAAATGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAGTTTAAATACTGTGGCGTCTATGCGGTGCAgtggtggt                                                                        >  1:2926723/1‑132 (MQ=255)

                                                                                                 cccTAGGGGACGCCAAATGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAGTTTAAATACTGTGGCGTCTATGCGGTGCAgtggtggt                                                                        <  2:2926723/132‑1 (MQ=255)

                                                                                                  ccTAGGGGACGCCAAATGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAGTTTAAATACTGTGGCGTCTATGCGGTGCAGTGGTGGTGAAGGTCGTAAAGGCGCtt                                                     >  1:2354162/1‑150 (MQ=255)

                                                                                                      ggggACGCCAAATGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAGTTTAAATACTGTGGCGTCTATGCGGTGCAGTGGTGGTGAAGGTCGTAAAGGCGCTTCACg                                                 >  1:4596128/1‑150 (MQ=255)

                                                                                                       gggACGCCAAATGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAGTTTAAATACTGTGGCGTCTATGCGGTGCAGTGGTGGTGAAGGTCGTAAAGGCGCTTCACGc                                                >  1:5280131/1‑150 (MQ=255)

                                                                                                            gCCAAATGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAGTTTAAATACTGTGGCGTCTATGCGGTGCAGTGGTGGTGAAGGTCGTAAAGGCGCTTCACGCCGAAc                                           <  2:5280131/150‑1 (MQ=255)

                                                                                                             gcAAATGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGa                                                                                                                                      <  1:2053509/57‑1 (MQ=18)

                                                                                                              cAAATGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAGTTTAATTAATGTGGCGTCTATGCGGTGCAGTGGTGGTGAAGGTCGTAAAGGCGCTTCACGCCGAACGt                                         >  2:3457576/1‑150 (MQ=255)

                                                                                                              cAAATGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAGTTTAAATACTGTGGCGTCTATGCGGTGCAGTGGTGGTGAAGGTCGTAAAGGCGCTTCACGCCGAACGt                                         >  1:2398158/1‑150 (MQ=255)

                                                                                                               aactGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAGTTTAAATACTGTGGCGTCTATGCGGTGCAGTGGTGGTGAAGGTCGTAAAGGCGCTTCACGCCGAACGTg                                        <  2:4359208/147‑1 (MQ=255)

                                                                                                               aaaTGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGtt                                                                                                                        <  1:4585098/70‑1 (MQ=31)

                                                                                                                 aTGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAGTTTAAATACTGTGGCGTCTATGCGGTGCAGTGGTGGTGAAGGTCGTAAAGGCGCTTCACGCCGAACGTGGt                                      <  2:2212768/150‑1 (MQ=255)

                                                                                                                  tGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAGTTTAAATACTGTGGCGTCTATGCGGTGCAGTGGTGGTGAAGGTCGTAAAGGCGCTTCACGCCGAACGTGGTa                                     <  2:3482471/150‑1 (MQ=255)

                                                                                                                   gCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAGTTTAAATACTGTGGCGTCTATGCGGTGCAGTGGTGGTGAAGGTCGTAAAGGCGCTTCACGCCGAACGTGGTaa                                    >  1:975506/1‑150 (MQ=255)

                                                                                                                     gggAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAGTTTAAATACTGTGGCGTCTATGCGGTGCAGTGGTGGTGAAGGTCGTAAAGGCGCTTCACGCCGAAAGAGGTAAAg                                  >  2:1556582/1‑150 (MQ=255)

                                                                                                                     gggAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAGATTAAATACTGTGGCGTCTATCCGGTGCAGTGGTGGTGAAGGTCGTAAAGGCGCTTCACGCCGAACGTGGTCAAg                                  >  2:1384509/1‑150 (MQ=255)

                                                                                                                         aTAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAGTTTAAATACTGTGGCGTCTATGCGGTGCAGTGGTGGTGAAGGTCGTAAAGGCGCTTCACGCCGAACGTGGTAAAGCTTc                              >  2:5325586/1‑150 (MQ=255)

                                                                                                                             cTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCATATTAAATAATCAGGCGTCGATCCGGTGCAGTGGTGTAGAAGGTCGTAAAGGCGCTTCACGCAGAACGTGGTAAAGATTAAAAt                          >  2:3811879/1‑150 (MQ=21)

                                                                                                                                aGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAGTTTAAATACTGTGGCGTCTATGCGGTGCCGTGGTGGGGACGGTCGTAAAGGCGCGTCACGCCGAACGGGGTAAAGCTTCAAATTGc                       >  2:792736/1‑150 (MQ=25)

                                                                                                                                aGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAGTTTAAATACTGTGGCGTCTATGCGGTGCAGTGGTGGTGAAGGTCGTAAAGGCGCTTCACGCCGAACGTGGTAAAGCTTCAAATTGc                       <  1:613923/150‑1 (MQ=255)

                                                                                                                                  ttGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAGTTTAAATACTGTGGCGTCTATGCGGTGCAGTGGTGGTGAAGGTCGTAAAGGCGCTTCACGCCGAACGTGGTAAAGCTTCAAATTGCgg                     <  2:4618913/150‑1 (MQ=255)

                                                                                                                                  ttGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAGTTTAAATACTGTGGCGTCTATGCGGTGCAGTGGTGGTGAAGGTCGTAAAGGCGCTTCACGCCGAACGTGGTAAAGCTTCAAATTGCgg                     <  2:4585550/150‑1 (MQ=255)

                                                                                                                                    ggTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAGTTTaaa                                                                                                       <  1:3877978/66‑1 (MQ=35)

                                                                                                                                    ggTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAGTTTAAATACTGTGGCGTCTATGCGGTGCAGTGGTGGTGAAGGTCGTAAAGGCGCTTCACGCCGAACGTGGTAAAGCTTCAAATTGCGGGa                   <  1:1454799/150‑1 (MQ=255)

                                                                                                                                          gCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAGTTTAAATACTGTGGCGTCTATGCGGTGCAGTGGTGGTGAAGGTCGTAAAGGCGCTTCACGCCGAACGTGGTAAAGCTTCAAATTGCGGGAATAGCt             >  1:5392721/1‑150 (MQ=255)

                                                                                                                                          gCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAGTTTAAATACTGTGGCGTCTATGCGGTGCAGTGGTGGTGAAGGTCGTAAAGGCGCTTCACGCCGAACGTGGGAAAGCTGCCAATTGCGGGAATAGCt             >  2:4025789/1‑150 (MQ=255)

                                                                                                                                           cACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAGTTTAAATACTGTGGCGTCTATGCGGTGCAGTGGTGGTGAAGGTCGTAAAGGCGCTTCACGCCGACCGTGGTAAAGCTTCAAATTGCGGGAATAGCTc            >  1:4341784/1‑150 (MQ=255)

                                                                                                                                            aCGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAGTTTAAATACTGTGGCGTCTATGCGGTGCAGTGGTGGTGAAGGTCGTAAAGGCGCTTCACGCCGAACGTg                                        >  1:4912460/1‑121 (MQ=255)

                                                                                                                                            aCGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAGTTTAAATACTGTGGCGTCTATGCGGTGCAGTGGTGGTGAAGGTCGTAAAGGCGCTTCACGCCGAACGTg                                        <  2:4912460/121‑1 (MQ=255)

                                                                                                                                            aCGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAGTTTAAATACTGTGGCGTCTATGCGGTGCAGTGGTGGTGAAGGTCGTAAAGGCGCTTCACGCCGAACGTGGTAAAGCTTCAAATTGCGGGAATAGCTCa           <  2:134284/150‑1 (MQ=255)

                                                                                                                                            aCGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAGTTTAAATACTGTGGCGTCTATGCGGTGCAGTGGTGGTGAAGGTCGTAAAGGCGCTTCACGCCGAACGTGGTAAAGCTTCAAATTGCGGGAATAGCTCa           >  2:323715/1‑150 (MQ=255)

                                                                                                                                            aCGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAGTTTAAATACTGTGGCGTCTATGCGGTGCAGTGGTGGTGAAGGTCGTAAAGGCGCTTCACGCCGAACGTGGTAAAGCTTCAAATTGCGGGAATAGCTCa           <  1:2127475/150‑1 (MQ=255)

                                                                                                                                            aCGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAGTTTAAATACTGTGGCGTCTATGCGGTGCAGTGGTGGTGAAGGTCGTAAAGGCGCTTCACGCCGAACGTGGTAAAGCTTCAAATTGCGGGAATAGCTCa           <  2:2354162/150‑1 (MQ=255)

                                                                                                                                                ccTTGGCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAGTTTAAATACTGTGGCGTCTATGCGGTGCAGTGGTGGTGAAGGTCGTAAAGGCGCTTCAc                                                  >  1:2677711/1‑107 (MQ=255)

                                                                                                                                                ccTTGGCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAGTTTAAATACTGTGGCGTCTATGCGGTGCAGTGGTGGTGAAGGTCGTAAAGGCGCTTCAc                                                  <  2:2677711/107‑1 (MQ=255)

                                                                                                                                                ccTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAGTTTAAATACTGTGGCGTCTATGCGGTGCAGTGGTGGTGAAGGTCGTAAAGGCGCTTCACGCCGAACGTGGTAAAGCTTCAAATTGCGGGaa                  <  2:1873653/139‑1 (MQ=255)

                                                                                                                                                ccTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAGTTTAAATACTGTGGCGTCTATGCGGTGCAGTGGTGGTGAAGGTCGTAAAGGCGCTTCACGCCGAACGTGGTAAAGCTTCAAATTGCGGGaa                  >  1:1873465/1‑139 (MQ=255)

                                                                                                                                                 cTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAGTTTAAATACTGTGGCGTCTATGCGGTGCAGTGGTGGTGAAGGTCGTAAAGGCGCTTCACGCCGAACGTGGTAAAGCTTCAAATTGCGGGAATAGCTCAGTTgg      <  1:4048329/150‑1 (MQ=255)

                                                                                                                                                 cTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAGTTTAAAAACTGTGGCGTCTATGCGGTGCAGTGGTGGTGAAGGTCGTAAAGGCGCTTCACGCCGAACGTGGTAAAGCTTCAAAATGCGGGAATAGATCAGTTgg      >  1:4889866/1‑150 (MQ=255)

                                                                                                                                                 cTTGCCAAGGTCGGGGTCGAGAGTTCGAGTCTCTTTTCCCGCTCCAGTTTAAATACTGTGGCGTCTATGCGGTGCAGTGGTGGTGAAGGTCGTAAAGGCGCTTCACGCCGAACGTGGTAAAGCTTCAAATTGCGGGAATAGCTCAGTTgt      <  2:2789160/150‑2 (MQ=255)

                                                                                                                                                  ttGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAGTTTAAATACTGTGGCGTCTATGCGGTGCAGTGGTGGTGAAGGTCGTAAAGGCGCTTCACGCCGAACGTGGTAAAGCTTCAAATTGCGGGAATAGCTCAGTTGGt     <  2:4596128/150‑1 (MQ=255)

                                                                                                                                                  ttGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAGTTTAAATACTGTGGCGTCTATGCGGTGCAGTGGTGGTGAAGGTCGTAAAGGCGCTTCACGCCGAACGTGGTAAAGCTTCAAATTGCGGGAATAGCTCAGTTGGt     <  1:5323596/150‑1 (MQ=255)

                                                                                                                                                   tGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAGTTTAAATACTGTGGCGTCTATGCGGTGCAGTGGTGGTGAAGGTCGTAAAGGCGCTTCACGCCGAACGTGGTAAAGCTTCAAATTGCGGGAATAGCTCAGTTGGTa    <  2:4952507/150‑1 (MQ=255)

                                                                                                                                                   tGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAGTTTAAATACTGTGGCGTCTATGCGGTGCAGTGGTGGTGAAGGTCGTAAAGGCGCTTCACGCCGAACGTGGTAAAGCTTCAAATTGCGGGAATAGCTCAGTTGGTa    <  1:4253650/150‑1 (MQ=255)

                                                                                                                                                    gCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAGTTTAAATACTGTGGCGTCTATGCGGTGCAGTGGTGGTGAAGGTCGTAAAGGCGCTTCACGCCGAACGTGGTAAAGCTTCAAAATGCGGGAATAGCTCAGTTGGTag   >  1:2857391/1‑150 (MQ=255)

                                                                                                                                                     ccAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAGTTTAAATACTGTGGCGTCTATGCGGTGCAGTGGTGGTGAAGGTCGTAAAGGCGCTTCACGCCGAACGTGGTAAAGCTTCAAATTGCGGGAATAGCTCAGTTGGTaga  <  1:3320258/150‑1 (MQ=255)

                                                                                                                                                     ccAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAGTTTAAATACTGTGGCGTCTATGCGGTGCAGTGGTGGTGAAGGTCGTAAAGGCGCTTCACGCCGAACGTGGTAAAGCTTCAAATTGCGGGAATAGCTCAGTTGGTaga  <  1:2626510/150‑1 (MQ=255)

GAGGTTCATGTTCTGGTGCACTGAAAAGTGTTGTGTGTCCCCTTCGTCTAGTGGCCTAGGACACCGCCCTTTCACGGCGGTAACAGGGGTTCGAGTCCCCTAGGGGACGCCAAATGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAGTTTAAATACTGTGGCGTCTATGCGGTGCAGTGGTGGTGAAGGTCGTAAAGGCGCTTCACGCCGAACGTGGTAAAGCTTCAAATTGCGGGAATAGCTCAGTTGGTAGA  >  NC_002947/2104815‑2105113

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 11 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`