BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	2,106,663	C→T	5.1%	E62E (GAG→GAA)	PP_1879 ←	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	2,106,663	0	C	T	5.1%	427.9 / 7.4	135	E62E (GAG→GAA)	PP_1879	hypothetical protein
Reads supporting (aligned to +/- strand): ref base C (68/60); new base T (3/4); total (71/64)
Fisher's exact test for biased strand distribution p-value = 7.08e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 137 total aligned reads displayed.

TCAATAAGCTTGTCGACCGGTACCAATATGAATAGCTCTTCAGGTTCGATAGGCTTGCTGAAGGTCTTCTTGTCGACCAGTACGTCATTCCAGTAGAGGAAAAGGTATTCCTCATCTATTTCCGGGTGCGAATTTGCCCACGGGTGGAACTCCACCCGCAGGGGGGAATAGGTTGCCCGGAACTCGACCGTGTTGGGTTTCCCCCCTTCAACATCGTCCACCACGCTTGGTACGGTTGGGGGGACAAGAGAGGCCGAGTCCAGTTCAGGGGTTGGAGTTCTGCGGTTACCGGAGCGCTT  >  NC_002947/2106514‑2106812

tCAATAAGCTTGTCGACCGGTACCAATATGAATAGCTCGTCAGGTTCGATAGGCTTGCTGAAGGTCTTCTTGTCGACCAGTACGTCATTCCAGTAGAGGAAAAGGTATTCCTCATCTATTTCCGGGTGCGAATTTGCCCACGGGTGGAAc                                                                                                                                                       <  2:3953511/150‑1 (MQ=255)

  aaTAAGCTTGTCGACCGGTACCAATATGAATAGCTCTTCAGGTTCGATAGGCTTGCTGAAGGTCTTCTTGTCGACCAGTACGTCATTCCAGTAGAGGAAAAGGTATTCCTCATCTATTTCCGGGTGCGAATTTGCCCACGGGTGGAACTc                                                                                                                                                     >  2:3394042/1‑150 (MQ=255)

    tAAGCTTGTCGACCGGTACCAATATGAATAGCTCTTCAGGTTCGATAGGCTTGCTGAAGGTCTTCTTGTCGACCAGTACGTCATTCCAGTAGAGGAAAAGGTATTCCTCATCTATTTCCGGGTGCGAATTTGCCCACGGGTGGAACTCCa                                                                                                                                                   >  1:1529532/1‑150 (MQ=255)

         ttGTCGACCGGTACCAATATGAATAGCTCTTCAGGTTCGATAGGCTTGCTGAAGGTCTTCTTGTCGACCAGTACGTCATTCCAGTAGAGGAAAAGGTATTCCTCATCTATTTCCGGGTGCGAATTTGCCCACGGGTGGAACTCCACCCGc                                                                                                                                              <  2:4513090/150‑1 (MQ=255)

           gTCGACCGGTACCAATATGAATAGCTCTTCAGGTTCGATAGGCTTGCTGAAGGTCTTCTTGTCGACCAGTACGTCATTCCAGTAGAGGAAAAGGTATTCCTCATCTATTTCCGGGTGCGAATTTGCCCACGGGTGGAGTTCCACCCgtgg                                                                                                                                            <  2:5139106/150‑4 (MQ=255)

           gTCGACCGGTACCAATATGAATAGCTCTTCAGGTTCGATAGGATTGCTGAAGGTCTTCTTGTCGACCAGTACGTCATTCCAGTAGAGGAAAAGGTATTCCTCATCTATTTCCGGGTGCGAATTTGCCCACGGGTGGAACTCCACCCGCAg                                                                                                                                            <  2:1922081/150‑1 (MQ=255)

            tCGACCGGTACCAATATGAATAGCTCTTCAGGTTCGATAGGCTTGCTGAAGGTCTTCTTGTCGACCAGTACGTCATTCCAGTAGAGGAAAAGGTATTCCTCATCTATTTCCGGGTGCGAATTTGCCCACGGGTGGAACTCCACCCGCAgg                                                                                                                                           <  2:968444/150‑1 (MQ=255)

            tCGACCGGTACCAATATGAATAGCTCTTCAGGTTCGATAGGCTTGCTGAAGGTCTTCTTGTCGACCAGTACGTCATTCCAGTAGAGGAAAAGGTATTCCTCATCTATTTCCGGGTGCGAATTTGCCCACGGGTGGAACTCCACCCGCAgg                                                                                                                                           <  2:2087144/150‑1 (MQ=255)

            tCGACCGGTACCAATATGAACAGCTCTTCAGGTTCGATAGGCTTGCTGAAGGTCTTCTTGTCGACCAGTACGTCATTCCAGTAGAGGAAAAGGTATTCCTCATCTATTTCCGGGTGCGAATTTGCCCACGGGTGGAACTCCACCCGCAgg                                                                                                                                           <  1:2435456/150‑1 (MQ=255)

             cGACCGGTACCAATATGAATAGCTCTTCAGGTTCGATAGGCTTGCTGAAGGTCTTCTTGTCGACCAGTACGTCATTCCAGTAGAGGAAAAGGTATTCCTCATCTATTTCCGGGTGCGAATTTGCCCACGGGTGGAACTCCACCCGCAggg                                                                                                                                          <  1:5410399/150‑1 (MQ=255)

              gACCGGTACCAATATGAATAGCTCTTCAGGTTCGATAGGCTTGCTGAAGGTCTTCTTGTCGACCAGTACGTCATTCCAGTAGAGGAAAAGGTATTCCTCATCTATTTCCGGGTGCGAATTTGCCCACGGGTGGAACTCCACCCGCAgggg                                                                                                                                         <  2:794921/150‑1 (MQ=255)

              gACCGGTACCAATATGAATAGCTCTTCAGGTTCGATAGGCTTGCTGAAGGTCTTCTTGTCGACCAGTACGTCATTCCAGTAGAGGAAAAGGTATTCCTCATCTATTTCCGGGTGCGAATTTGCCCACGGGTGGAACTCCACCCGCAgggg                                                                                                                                         >  2:1672460/1‑150 (MQ=255)

                    tACCAATATGAATAGCTCTTCAGGTTCGATAGGCTTGCTGAAGGTCTTCTTGTCGACCAGTACGTCATTCCAGTAGAGGAAAAGGTATTCCTCATCTATTTCCGGGTGCGAATTTGCCCACGGGTGGAACTCCACCCGCAGGGGGGAATa                                                                                                                                   <  1:960214/150‑1 (MQ=255)

                    tACCAATATGAATAGCTCTTCAGGTTCGATAGGCTTGCTGAAGGTCTTCTTGTCGACCAGTACGTCATTCCAGTAGAGGAAAAGGTATTCCTCATCTATTTCCGGGTGCGAATTTGCCCACGGGTGGAACTCCACCCGCAGGGGGGAATa                                                                                                                                   <  1:4582030/150‑1 (MQ=255)

                     aCCAATATGAATAGCTCTTCAGGTTCGATAGGCTTGCTGAAGGTCTTCTTGTCGACCAGTACGTCATTCCAGTAGAGGAAAAGGTATTCCTCATCTATTTCCGGGTGCGAATTTGCCCACGGGTGGAACTCCACCCGCAGGGGGGAATAg                                                                                                                                  <  2:967804/150‑1 (MQ=255)

                     aCCAATATGAATAGCTCTTCAGGTTCGATAGGCTTGCTGAAGGTCTTCTTGTCGACCAGTACGTCATTCCAGTAGAGGAAAAGGTATTCCTCATCTATTTCCGGGTGCGAATTTGCCCACGGGTGGAACTCCACCCGCAGGGGGGAATAg                                                                                                                                  <  2:2142826/150‑1 (MQ=255)

                             gAATAGCTCTTCAGGTTCGATAGGCTTGCTGAAGGTCTTCTTGTCGACCAGTACGTCATTCCAGTAGAGGAAAAGGTATTCCTCATCTATTTCCGGGTGCGAATTTGCCCACGGGTGGAACTCCACCCGCAGGGGGGAATAGGTTGCCCg                                                                                                                          <  1:1740446/150‑1 (MQ=255)

                               aTAGCTCTTCAGGTTCGATAGGCTTGCTGAAGGTCTTCTTGTCGACCAGTACGTCATTCCAGTAGAGGAAAAGGTATTCCTCATCTATTTCCGGGTGCGAATTTGCCCACGGGTGGAACTCCACCCGCAGGGGGGAATAGGTTGCCCGGa                                                                                                                        >  1:3190042/1‑150 (MQ=255)

                                 aGCTCTTCAGGTTCGATAGGCTTGCTGAAGGTCTTCTTGTCGACCAGTACGTCATTCCAGTAGAGGAAAAGGTATTCCTCATCTATTTCCGGGTGCGAATTTGCCCACGGGTGGAACTCCACCCGCAGGGGGGAATAGGTTGCCCGGAAc                                                                                                                      <  2:4105574/150‑1 (MQ=255)

                                  gCTCTTCAGGTTCGATAGGCTTGCTGAAGGTCTTCTTGTCGACCAGTACGTCATTCCAGTAGAGGAAAAGGTATTCCTCATCTATTTCCGGGTGCGAATTTGCCCACGGGTGGAACTCCACCCGCAGGGGGGAATAGGTTGCCCGCAACt                                                                                                                     >  2:3603002/1‑150 (MQ=255)

                                    tctTCAGGTTCGATAGGCTTGCTGAAGGTCTTCTTGTCGACCAGTACGTCATTCCAGTAGAGGAAAAGGTATTCCTCATCTATTTCCGGGTGCGAATTTGCCCACGGGTGGAACTCCACCCGCAGGGGGGAATAGGTTGCCCGGAACTCg                                                                                                                   >  2:3114447/1‑150 (MQ=255)

                                      ttCAGGTTCGATAGGCTTGCTGAAGGTCTTCTTGTCGACCAGTACGTCATTCCAGTAGAGGAAAAGGTATTCCTCATCTATTTCCGGGTGCGAATTTGCCCACGGGTGGAACTCCACCCGCAGGGGGGAATAGGTTGCCCGGAACTCGAc                                                                                                                 >  2:1220818/1‑150 (MQ=255)

                                      ttCAGGTTCGATAGGCTTGCTGAAGGTCTTCTTGTCGACCAGTACGTCATTCCAGTAGAGGAAAAGGTATTCCTCATCTATTTCCGGGTGCGAATTTGCCCACGGGTGGAACTCCACCCGCAGGGGGGAATAGGTTGCCCGGAACTCGAc                                                                                                                 >  1:4060404/1‑150 (MQ=255)

                                       tCAGGTTCGATAGGCTTGCTGAAGGTCTTCTTGTCGACCAGTACGTCATTCCAGTAGAGGAAAAGGTATTCCTCATCTATTTCCGGGTGCGAATTTGCCCACGGGTGGAACTCCACCCGCAGGGGGGAATAGGTTGCCCGGAACTCGAcc                                                                                                                >  2:3943891/1‑150 (MQ=255)

                                        cAGGTTCGATAGGCTTGCTGAAGGTCTTCTTGTCGACCAGTACGTCATTCCAGTAGAGGAAAAGGTATTCCTCATCTATTTCCGGGTGCGAATTTGCCCACGGGTGGAACTCCACCCGCAGGGGGGACTAGGTTGCCCGGAACTCGACCg                                                                                                               >  1:4114939/1‑150 (MQ=255)

                                            ttCGATAGGCTTGCTGAAGGTCTTCTTGTCGACCAGTACGTCATTCCAGTAGAGGAAAAGGTATTCCTCATCTATTTCCGGGTGCGAATTTGCCCACGGGTGGAACTCCACCCGCAggggg                                                                                                                                        <  1:3668302/121‑1 (MQ=255)

                                            ttCGATAGGCTTGCTGAAGGTCTTCTTGTCGACCAGTACGTCATTCCAGTAGAGGAAAAGGTATTCCTCATCTATTTCCGGGTGCGAATTTGCCCACGGGTGGAACTCCACCCGCAggggg                                                                                                                                        >  2:3668302/1‑121 (MQ=255)

                                            ttCGATAGGCTTGCTGAAGGTCTTCTTGTCGACCAGTACGTCATTCCAGTAGAGGAAAAGGTATTCCTCATCTATTTCCGGGTGCGAATTTGCCCACGGGTGGAACTCCACCCGCAGGGGGGAATAGGTTGCCCGGAACTCGACCGTGtt                                                                                                           >  2:3404224/1‑150 (MQ=255)

                                                aTAGGCTTGCTGAAGGTCTTCTTGTCGACCAGTACGTCATTCCAGTAGAGGAAAAGGTATTCCTCATCTATTTCCGGGTGCGAATTTGCCCACGGGTGGAACTCCACCCGCAGGGGGGAATAGGTTGCCCGGAACTCGACCGTGTTGGGt                                                                                                       >  2:4660513/1‑150 (MQ=255)

                                                   ggCTTGCTGAAGGTCTTCTTGTCGACCAGTACGTCATTCCAGTAGAGGAAAAGGTATTCCTCATCTATTTCCGGGTGCGAATTTGCCCACGGGTGGAACTCCACCCGCAGGGGGGAATAGGTTGCCCGGAACTCGACCGTGTTGGGTTTc                                                                                                    <  1:4377723/150‑1 (MQ=255)

                                                    gCTTGCTGAAGGTCTTCTTGTCGACCAGTACGTCATTCCAGTAGAGGAAAAGGTATTCCTCATCTATTTCCGGGTGCGAATTTGCCCACGGGTGGAACTCCACCCGCAGGGGGGAATAGGTTGCCCGGAACTCGACCGTGTTGGGTTTcc                                                                                                   <  2:5066622/150‑1 (MQ=255)

                                                     cTTGCTGAAGGTCTTCTTGTCGACCAGTACGTCATTCCAGTAGAGGAAAAGGTATTCCTCATCTATTTCCGGGTGCGAATTTGCCCACGGGTGGAACTCCACCCGCAGGGGGGAATAGGTTGCCCGGAACTCGACCGTGTTGGGTTTccc                                                                                                  <  1:4953518/150‑1 (MQ=255)

                                                      ttGCTGAAGGTCTTCTTGTCGACCAGTACGTCATTCCAGTAGAGGAAAAGGTATTCCTCATCTATTTCCGGGTGCGAATTTGCCCACGGGTGGAACTCCACCCGCAGGGGGGAATAGGTTGCCCGGAACTCGACCGTGTTGGGTTTcccc                                                                                                 <  1:1672460/150‑1 (MQ=255)

                                                          tGAAGGTCTTCTTGTCGACCAGTACGTCATTCCAGTAGAGGAAAAGGTATTCCTCATCTATTTCCGGGTGCGAATTTGCCCACGGGTGGAACTCCACCCGCAGGGGGGAATAGGTTGCCCGGaa                                                                                                                       >  1:3183017/1‑124 (MQ=255)

                                                          tGAAGGTCTTCTTGTCGACCAGTACGTCATTCCAGTAGAGGAAAAGGTATTCCTCATCTATTTCCGGGTGCGAATTTGCCCACGGGTGGAACTCCACCCGCAGGGGGGAATAGGTTGCCCGGAACTCGACCGTGTTGGGTTTCCCCCCtt                                                                                             >  1:1053392/1‑150 (MQ=255)

                                                          tGAAGGTCTTCTTGTCGACCAGTACGTCATTCCAGTAGAGGAAAAGGTATTCCTCATCTATTTCCGGGTGCGAATTTGCCCACGGGTGGAACTCCACCCGCAGGGGGGAATAGGTTGCCCGGAACTCGACCGTGTTGGGTTTCCCCCCtt                                                                                             >  1:4913324/1‑150 (MQ=255)

                                                           gAAGGTCTTCTTGTCGACCAGTACGTCATTCCAGTAGAGGAAAAGGTATTCCTCATCTATTTCCGGGTGCGAATTTGCCCACGGGTGGAACTCCACCCGCAGGGGGGAATAGGTTGCCCGGAACTCGACCGTGTTGGGTTTCCCCCCTTc                                                                                            >  2:3580295/1‑150 (MQ=255)

                                                            aaGGTCTTCTTGTCGACCAGTACGTCATTCCAGTAGAGGAAAAGGTATTCCTCATCTATTTCCGGGTGCGAATTTGCCCACGGGTGGAACTCCACCCGCAGGGGGGAATAGGTTGCCCGGAACTCGACCGTGTTGGGTTTCCCCCCTTCa                                                                                           >  1:4277575/1‑150 (MQ=255)

                                                            aaGGTCTTCTTGTCGACCAGAACGTCATTCCAGTAGAGGAAAAGGTATTCCTCATCTATTTCCGGGTGCGAATTTGCCCACGGGTGGAACTCCACCCGCAGGGGGGAATAGGTTGCCCGGAACTCGACCGTGTTGGGTTTCCCCCCTTCa                                                                                           >  2:3095762/1‑150 (MQ=255)

                                                              ggTCTTCTTGTCGACCAGTACGTCATTCCAGTAGAGGAAAAGGTATTCCTCATCTATTTCCGGGTGCGAATTTGCCCACGGGTGGAACTCCACCCGCAGGGGGGAATAGGTTGCCCGGAACTCGACCGTGTTGGGTTTcccccccacggg                                                                                         >  2:1842395/1‑144 (MQ=255)

                                                                  ttcttGTCGACCAGTACGTCATTCCAGTAGAGGAAAAGGTATTCCTCATCTATTTCCGGGTGCGAATTTGCCCACGGGTGGAACTCCACCCGCAGGGGGGAATAGGTTGCCCGGAACTCGACCGTGTTGGGTTTCCCCCCTTCAACATCg                                                                                     >  2:3512636/1‑150 (MQ=255)

                                                                     ttGTCGACCAGTACGTCATTCCAGTAGAGGAAAAGGTATTCCTCATCTATTTCCGGGTGCGAATTTGCCCACGGGTGGAACTCCACCCGCAGGGGGGAATAGGTTGCCCGGAACTCGACCGTGTTg                                                                                                          <  2:4810053/126‑1 (MQ=255)

                                                                     ttGTCGACCAGTACGTCATTCCAGTAGAGGAAAAGGTATTCCTCATCTATTTCCGGGTGCGAATTTGCCCACGGGTGGAACTCCACCCGCAGGGGGGAATAGGTTGCCCGGAACTCGACCGTGTTGGGTTTCCCCCCTTCAACATCGTcc                                                                                  >  2:4240844/1‑150 (MQ=255)

                                                                       gTCGACCAGTACGTCATTCCAGTAGAGGAAAAGGTATTCCTCATCTATTTCCGGGTGCGAATTTGCCCACGGGTGGAACTCCACCCGCAGGGGGGAATAGGTTGCCCGGAACTCGACCGTGTTGGGTTTCCCCCCTTCAACATCGTccac                                                                                >  2:2213627/1‑150 (MQ=255)

                                                                        tCGACCAGTACGTCATTCCAGTAGAGGAAAAGGTATTCCTCATCTATTTCCGGGTGCGAATTTGCCCACGGGTGGAACTCCACCCGCAGGGGGGAATAGGTTGCCCGGAACTCGACCGTGTTGGGTTTCCCCCCTTCAACATCGTccac                                                                                >  1:285270/1‑149 (MQ=255)

                                                                          gACCAGTACGTCATTCCAGTAGAGGAAAAGGTATTCCTCATCTATTTCCGGGTGCGAATTTGCCCACGGGTGGAACTCCACCCGCAGGGGGGAATAGGTTGCCCGGAACTCGACCGTGTTGGGTTTCCCCCCTTCAACATCGTCcaccac                                                                             >  1:2642002/1‑150 (MQ=255)

                                                                                           aGTAGAGGAAAAGGTATTCCTCATCTATTTCCGGGTGCGAATTTGCCCACGGGTGGAACTCCACCCGCAGGGGGGAATAGGTTGCCCGGAACTCGACCGTGTTGGGTTTCCCCCCTTCAACATCGTCCACCACGCTTGGTACGGTTgggg                                                            <  2:2850921/150‑1 (MQ=255)

                                                                                           aGTAGAGGAAAAGGTATTCCTCATCTATTTCCGGGTGCGAATTTGCCCACGGGTGGAACTCCACCCGCAGGGGGGAATAGGTTGCCCGGAACTCGACCGTGTTGGGTTTCCCCCCTTCAACATCGTCCACCACGCTTGGTACGGTTgggg                                                            <  1:1620702/150‑1 (MQ=255)

                                                                                           aGTAGAGGAAAAGGTATTCCTCATCTATTTCCGGGTGCGAATTTGCCCACGGGTGGAACTCCACCCACAGGGGGGAATAGGTTGCCCGGAACTCGACCGTGTTGGGTTTCCCCCCTTCAACATCGTCCACCACGCTTGGTACGGTTgggg                                                            <  1:97106/150‑1 (MQ=255)

                                                                                              agagGAAAAGGTATTCCTCATCTATTTCCGGGTGCGAATTTGCCCACGGGTGGAACTCCACCCGCAGGGGGGAATAGGTTGCCCGGAACTCGACCGTGTTGGGTTTCCCCCCTTCAACATCGTCCACCACGCTTGGTACGGTTGGGGGGa                                                         <  2:5037191/150‑1 (MQ=255)

                                                                                              agagGAAAAGGTATTCCTCATCTATTTCCGGGTGCGAATTTGCCCACGGGTGGAACTCCACCCGCAGGGGGGAATAGGTTGCCCGGAACTCGACCGTGTTGGGTTTCCCCCCTTCAACATCGTCCACCACGCTTGGTACGGTTGGGGGGa                                                         <  1:5317744/150‑1 (MQ=255)

                                                                                                  gAAAAGGTATTCCTCATCTATTTCCGGGTGCGAATTTGTCCACGGGTGGAACTCCACCCGCAGGGGGGAATAGGTTGCCCGGAACTCGACCGTGTTGGGTTTCCCCCCTTCAACATCGTCCACCACGCTTGGTACGGTTGGGGGGACAag                                                     >  2:863124/1‑150 (MQ=255)

                                                                                                  gAAAAGGTATTCCTCATCTATTTCCGGGTGCGAATTTGCCCACGGGTGGAACTCCACCCGCAGGGGGGAATAGGTTGCCCGGAACTCGACCGTGTTGGGTTTCCCCCCTTCAACATCGTCCACCACGCTTGGTACGGTTGGGGGGACAag                                                     >  1:1211105/1‑150 (MQ=255)

                                                                                                   aaaaGGTATTCCTCATCTATTTCCGGGTGCGAATTTGCCCACGGGTGGAACTCCACCCGCAGGGGGGAATAGGTTGCCCGGAACTCGACCGTGTTGGGTTTCCCCCCTTCAACATCGTCCACCACGCTTGGTACGGTTGGGGGGACAaga                                                    <  2:2126086/150‑1 (MQ=255)

                                                                                                   aaaaGGTATTCCTCATCTATTTCCGGGTGCGAATTTGCCCACGGGTGGAACTCCACCCGCAGGGGGGAATAGGTTGCCCGGAACTCGACCGTGTTGGGTTTCCCCCCTTCAAAATCGTCCACCACGCTTGGTACGGTTGGGGGGACAaga                                                    >  2:4147685/1‑150 (MQ=255)

                                                                                                    aaaGGTATTCCTCATCTATTTCCGGGTGCGAATTTGCCCACGGGTGGAACTCCACCCGCAGGGGGGAATAGGTTGCCCGGAACTCGACCGTGTTGGGTTTCCCCCCTTCAACATCGTCCACCACGCTTGGTACGGTTGGGGGGACAagag                                                   <  1:4602682/150‑1 (MQ=255)

                                                                                                    aaaGGTATTCCTCATCTATTTCCGGGTGCGAATTTGCCCACGGGTGGAACTCCACCCGCAGGGGGGAATAGGTTGCCCGGAACTCGACCGTGTTGGGTTTCCCCCCTTCAACATCGTCCACCACGCTTGGTACGGTTGGGGGGACAagag                                                   >  2:4642429/1‑150 (MQ=255)

                                                                                                      aGGTAGTCCTCATCTATTTCCGGGTGCGAATTTGCCCACGGGTGGAACTCCACCCGCAGGGGGGAATAGGTTGCCCGGAACTCGACCGTGTTGGGTTTCCCCCCTTCAACATCGTCCACCACGCTTGGTACGGTTGGGGGGACAagagag                                                 <  1:1762201/150‑1 (MQ=255)

                                                                                                         tATTCCTCATCTATTTCCGGGTGCGAATTTGCCCACGGGTGGAACTCCACCCGCAGGGGGGAATAGGTTGCCCGGAACTCGACCGTGTTGGGTTTCCCCCCTTCAACATCGTCCACCACGCTTGGTACGGTTGGGGGGACAAGAGAGGcc                                              <  1:3417020/150‑1 (MQ=255)

                                                                                                            tCCTCATCTATTTCCGGGTGCGAATTTGCCCACGGGTGGAACTCCACCCGCAGGGGGGAATAGGTTGCCCGGAACTCGACCGTGTTGGGTTTCCCCCCTTCAACATCGTCCACCACGCTTGGTACGGTTGGGGGGACAAGAGAGGCCGAg                                           <  2:2461099/150‑1 (MQ=255)

                                                                                                            tCCTCATCTATTTCCGGGTGCGAATTTGCCCACGGGTGGAACTCCACCCGCA‑GGGGGAATAGGTTGCCCGGAACTCGACCGTGTTGGGTTTCCCCCCTTCAACATCGTCCACCACGCTTGGTACGGTTGGGGGGACAAGAGAGGCCGAGt                                          <  2:2249646/150‑1 (MQ=255)

                                                                                                             ccTCATCTATTTCCGGGTGCGAATTTGCCCACGGGTGGAACTCCACCCGCAGGGGGGAATAGGTTGCCCGGAACTCGACCGTGTTGGGTTTCCCCCCTTCAACATCGTCCACCACGCTTGGTACGGTTGGGGGGACAAGAGAGGCCGAGt                                          >  1:3326365/1‑150 (MQ=255)

                                                                                                             ccTCATCTATTTCCGGGTGCGAATTTGCCCACGGGTGGAACTCCACCCGCAGGGGGGAATAGGTTGCCCGGAACTCGACCGTGTTGGGTTTCCCCCCTTCAACATCGTCCACCACGCTTGGTACGGTTGGGGGGACAAGAGAGGCCGAGt                                          >  1:1346688/1‑150 (MQ=255)

                                                                                                             ccTCATCTATTTCCGGGTGCGAATTTGCCCACGGGTGGAACTCCACCCGCAGGGGGGAATAGGTTGCCCGGAACTCGACCGTGTTGGGTTTCCCCCCTTCAACATCGTCCACCACGCTTGGTACGGTTGGGGGGACAAGAGAGGCCGAGt                                          >  1:1346674/1‑150 (MQ=255)

                                                                                                              cTCATCTATTTCCGGGTGCGAATTTGCCCACGGGTGGAACTCCACCCGCCGGGGGGAATAGGTTGCCCGGAACTCGACCGTGTTGGGTTTCCCCCCTTCAACATCGTCCACCACGCTTGGTACGGTTGGGGGGACAAGAGAGGCCGAGTc                                         <  2:3140907/150‑1 (MQ=255)

                                                                                                              cTCATCTATTTCCGGGTGCGAATTTGCCCACGGGTGGAACTCCACCCGCAGGGGGGAATAGGTTGCCCGGAACTCGACCGTGTTGGGTTTCCCCCCTTCAACATCGTCCACCACGCTTGGTACGGTTGGGGGGACAAGAGAGGCCGAGTc                                         >  2:661390/1‑150 (MQ=255)

                                                                                                              cTCATCTATTTCCGGCAGCGAATTTGCCAACGGGTGGAACTCCACCCGCAGGGCGGAATAGGTTGCCCGGAACACGACCGAGATGGGTTTCCCCCCTACAACATCGTCCACCACGCTTGCTACGGTACGGGGCACAAGAGAGGCCGAgac                                         >  2:661210/1‑148 (MQ=255)

                                                                                                               tCATCTATTTCCGGGTGCGAATTTGCCCACGGGTGGAACTCCACCCGCAGGGGGGAATAGGTTGCCCGGAACTCGACCGTGTTGGGTTTCCCCCCTTCAACATCGTCCACCACGCTTGGTACGGTTGGGGGGACAAGAGAGGCCGAGTcc                                        >  1:1095/1‑150 (MQ=255)

                                                                                                                 aTCTATTTCCGGGTGCGAATTTGCCCACGGGTGGAACTCCACCCGCAGGGGGGAATAGGTTGCCCGGAACTCGACCGTGTTGGGTTTCCCCCCTTCAACATCGTCCACCACGCTTGGTACGGTTGGGGGGACAAGAGAGGCCGAGTCCAg                                      >  1:3524066/1‑150 (MQ=255)

                                                                                                                 aTCTATTTCCGGGTGCGAATTTGCCCACGGGTGGAACTCCACCCGCAGGGGGGAATAGGTTGCCCGGAACTCGACCGTGTTGGGTTTCCCCCCTTCAACATCGCCCACCACGCTTGGTACGGTTGGGGGGACAAGAGAGGCCGAGTCCAg                                      >  1:5382646/1‑150 (MQ=255)

                                                                                                                   cTATTTCCGGGTGCGAATTTGCCCACGGGTGGAACTCCACCCGCAGGGGGGAATAGGTTGCCCGGAACTCGACCGTGTTGGGTTTCCCCCCTTCAACATCGTCCACCACGCTTGGTACGGTTGGGGGGACAAGAGAGGCCGAGTCCAGtt                                    <  2:794608/150‑1 (MQ=255)

                                                                                                                      tttCCGGGTGCGAATTTGCCCACGGGTGGAACTCCACCCGCAGGGGGGAATAGGTTGCCCGGAACTCGACCGTGTTGGGTTTCCCCCCTTCAACATCGTCCACCACGCTTGGTACGGTTGGGGGGACAAGAGAGGCCGAGTCCAGTTCAg                                 >  1:219725/1‑150 (MQ=255)

                                                                                                                       ttCCGGGTGCGAATTTGCCCACGGGTGGAACTCCACCCGCAGGGGGGAATAGGTTGCCCGGAACTCGACCGTGTTGGGTTTCCCCCCTTCAACATCGTCCACCACGCTTGGTACGGTTGGGGGGACAAGAGAGGCCGAGTCCAGTTCAgg                                >  2:3943768/1‑150 (MQ=255)

                                                                                                                       ttCCGGGTGCGAATTTGCCCACGGGTGGAACTCCACCCGCAGGGGGGAATAGGTTGCCCGGAACTCGACCGTGTTGGGTTTCCCCCCTTCAACATCGTCCACCACGCTTGGTACGGTTGGGGGGACAAGAGAGGCCGAGTCCAGTTCAgg                                >  2:1289282/1‑150 (MQ=255)

                                                                                                                        tCCGGGTGCGAATTTGCCCACGGGTGGAACTCCACCCGCAGGGGGGAATAGGTTGCCCGGAACTCGACCGTGTTGGGTTTCCCCCCTTCAACATCGTCCACCACGCTTGGTACGGTTGGGGGGACAAGAGAGGCCGAGTCCAGTTCAggg                               <  2:4277575/150‑1 (MQ=255)

                                                                                                                           gggTGCGAATTTGCCCACGGGTGGAACTCCACCCGCTGGGGGGAATAGGTTGCCCGGAACTCGACCGTGTTGGGTTTCCCCCCTTCAACATCGTCCACCACGCTTGGTACGGTTGGGGGGACAAGAGAGGCCGAGTCCAGTTCAGGGGtt                            <  2:3531484/150‑1 (MQ=255)

                                                                                                                           gggTGCGAATTTGCCCACGGGTGGAACTCCACCCGCAGGGGGGAATAGGTTGCCCGGAACTCGACCGTGTTGGGTTTCCCCCCTTCAACATCGTCCACCACGCTTGGTACGGTTGGGGGGACAAGAGAGGCCGAGTCCAGTTCAGGGGtt                            <  2:5213340/150‑1 (MQ=255)

                                                                                                                           gggTGCGAATTTGCCCACGGGTGGAACTCCACCCGCAGGGGGGAATAGGTTGCCCGGAACTCGACCGTGTTGGGTTTCCCCCCTTCAACATCGTCCACCACGCTTGGTACGGTTGGGGGGACAAGAGAGGCCGAGTCCAGTTCAGGGGtt                            <  2:2334352/150‑1 (MQ=255)

                                                                                                                           gggTGCGAATTTGCCCACGGGTGGAACTCCACCCGCAGGGGGGAATAGGTTGCCCGGAACTCGACCGTGTTGGGTTTCCCCCCTTCAACATCGTCCACCACGCTTGGTACGGTTGGGGGGACAAGAGAGGCCGAGTCCAGTTCAGGGGtt                            >  1:685711/1‑150 (MQ=255)

                                                                                                                             gTGCGAATTTGCCCACGGGTGGAACTCCACCCGCAGGGGGGAATAGGTTGCCCGGAACTCGACCGTGTTGGGTTTCCCCCCTTCAACATCGTCCACCACGCTTGGTACGGTTGGGGGGACAAGAGAGGCCGAGTCCAGTTCAGGGGTTgg                          <  1:2242905/150‑1 (MQ=255)

                                                                                                                               gCGAATTTGCCCACGGGTGGAACTCCACCCGCAGGGGGGAATAGGTTGCCCGGAACTCGACCGTGTTGGGTTTCCCCCCTTCAACATCGTCCACCACGCTTGGTACGGTTGGGGGGACAAGAGAGGCCGAGTCCAGTTCAGGGGTTGGAg                        <  1:2752602/150‑1 (MQ=255)

                                                                                                                                 gAATTTGCCCACGGGTGGAACTCCACCCGCAGGGGGGAATAGGTTGCCCGGAACTCGACCGTGTTGGGTTTCCCCCCTTCAACATCGTCCACCACGCTTGGTACGGTTGGGGGGACAAGAGAGGCCGAGTCCAGTTCAGGGGTTGGAGtt                      >  2:1991509/1‑150 (MQ=255)

                                                                                                                                      tGCCCACGGGTGGAACTCCACCCGCAGGGGGGAATAGGTTGCCCGGAACTCGACCGTGTTGGGTTTCCCCCCTTCAACATCGTCCACCACGCTTGGTACGGTTGGGGGGACAAGAGAGGCCGAGTCCAGTTCAGGGGTTGGAGTTCTGCg                 <  1:3321030/150‑1 (MQ=255)

                                                                                                                                      tGCCCACGGGTGGAACTCCACCCGCAGGGGGGAATAGGTTGCCCGGAACTCGACCGTGTTGGGTTTCCCCCCTTCAACATCGTCCACCACGCTTGGTACGGTTGGGGGGACAAGAGAGGCCGAGTCCAGTTCAGGGGTTGGAGTTCTGCg                 <  1:473677/150‑1 (MQ=255)

                                                                                                                                      cccctgcGGGTGGAGTTCCACCCGCAGGGGGGAATAGGTTGCCCGGAACTCGACCGTGTTGGGTTTCCCCCCTTCAACATCGTCCACCACGCTTGGTACGGTTGGGGGGACAAGAGAGGCCGAGTCCAGTTCAGGGGTTGGAGTTCTGCg                 >  2:2903187/7‑150 (MQ=255)

                                                                                                                                       ccctgcGGGTGGAGTTCCACCCGCAGGGGGGAATAGGTTGCCCGGAACTCGACCGTGTTGGGTTTCCCCCCTTCAACATCGTCCACCACGCTTGGTACGGTTGGGGGGACAAGAGAGGCCGa                                            <  2:2826743/117‑1 (MQ=255)

                                                                                                                                         ctgcGGGTGGAGTTCCACCCGCAGGGGGGAATAGGTTGCCCGGAACTCGACCGTGTTGGGTTTCCCCCCTTCAACATCGTCCACCACGCTTGGTACGGTTGGGGGGACAAGAGAGGCCGAGTCCAGTTCAGGGGTTGGAGTTCTGCGGtt              >  2:2392219/4‑150 (MQ=255)

                                                                                                                                         ctgcGGGTGGAACTCCACCCGCAGGGGGGAATAGGTTGCCCGGAACTCGACCGTGTTGGGTTTCCCCCCTTCAACATCGTCCACCACGCTTGGTACGGTTGGGGGGACAAGAGAGGCCGAGTCCAGTTCAGGGGTTGGAGTTCTGCGGtt              >  1:1156465/4‑150 (MQ=255)

                                                                                                                                         ccACGGGTGGAACTCCACCCGCAGGGGGGAATAGGTTGCCCGGAACTCGACCGTGTTGGGTTTCCCCCCTTCAACATCGTCCACCACGCTTGGTACGGTTGGGGGGACAAGAGAGGCCGAGTCCAGTTCAGGGGTTGGAGTTc                     >  1:3290042/1‑143 (MQ=255)

                                                                                                                                         ccACGGGTGGAACTCCACCCGCAGGGGGGAATAGGTTGCCCGGAACTCGACCGTGTTGGGTTTCCCCCCTTCAACATCGTCCACCACGCTTGGTACGGTTGGGGGGACAAGAGAGGCCGAGTCCAGTTCAGGGGTTGGAGTTc                     <  2:3290042/143‑1 (MQ=255)

                                                                                                                                         ccACGGGTGGAACTCCACCCGCAGGGGGGAAGAGGTTGCCCGGAACTCGACCGTGGTGGGTTTCCCCCCTTCCACATCGTCCACCACGCTTGGTACGGTTGGGGGGACAAGAGAGGCCGAGTCCAGTTCAGGGGTTGGAGTTCTGCGGtt              >  1:420440/1‑150 (MQ=255)

                                                                                                                                          cACGGGTGGAACTCCACCCGCAGGGGGGAATAGGTTGCCCGGAACTCGACCGTGTTGGGTTTCCCCCCTTCAACATCGTCCACCACGCTTGGTACGGTTGGGGGGACAAGAGAGGCCGAGTCCAGTTCAGGGGTTGGAGTTCTGCGGTTa             >  2:2761985/1‑150 (MQ=255)

                                                                                                                                           aCGGGTGGAACTCCACCCGCAGGGGGGAATAGGTTGCCCGGAACTCGACCGTGTTGGGTTTCCCCCCTTCAACATCGTCCACCACGCTTGGTACGGTTgggg                                                            >  1:289057/1‑102 (MQ=255)

                                                                                                                                           aCGGGTGGAACTCCACCCGCAGGGGGGAATAGGTTGCCCGGAACTCGACCGTGTTGGGTTTCCCCCCTTCAACATCGTCCACCACGCTTGGTACGGTTgggg                                                            <  2:289057/102‑1 (MQ=255)

                                                                                                                                             gggTGGAACTCCACCCGCAGGGGGGAATAGGTTGCCCGGAACTCGACCGTGTTGGGTTTCCCCCCTTCAACATCGTCCACCACGCTTGGTACGGTTGGGGGGACAAGAGAGGCCGAGTCCAGTTCAGGGGTTGGAGTTCTGCGGTTACCg          <  2:1529532/150‑1 (MQ=255)

                                                                                                                                                  gAACTCCACCCGCAGGGGGGAATAGGTTGCCCGGAACTCGACCGTGTTGGGTTTCCCCCCTTCAACATCGTCCACCACGCTTGGTACGGTTgg                                                              <  1:3531905/93‑1 (MQ=255)

                                                                                                                                                  gAACTCCACCCGCAGGGGGGAATAGGTTGCCCGGAACTCGACCGTGTTGGGTTTCCCCCCTTCAACATCGCCCACCACGCTTGGTACGGTTGGGGGGACAAGAGAGGCCGAGTCCAGTTCAGGGGTTGGAGTTCTGCGGTTACCGGAgcg     <  2:5382646/150‑1 (MQ=255)

                                                                                                                                                   aaCTCCACCCGCAGGGGGGAATAGGTTGCCCGGAACTCGACCGTGTTGGGTTTCCCCCCTTCAACATCGTCCACCACGCTTGGTACGGTTGGGGGGACAAGAGAGGCCGAGTCCAGTTCAGGGGTTGGAGTTCTGCGGTTACCGGAgcgc    >  2:1988738/1‑150 (MQ=255)

                                                                                                                                                   aaCTCCACCCGCAGGGGGGAATAGGTTGCCCGGAACTCGACCGTGTTGGGTTTCCCCCCTTCAACATCGTCCACCACGCTTGGTACGGTTGGGGGGACAAGAGAGGCCGAGTCCAGTTCAGGGGTTGGAGTTCTGCGGTTACCGGAgcgc    <  2:685711/150‑1 (MQ=255)

                                                                                                                                                     cTCCACCCGCAGGGGGGAATAGGTTGCCCGGAACTCGACCGTGTTGGGTTTCCCCCCTTCAACATCGTCCACCACGCTTGGTACGGTTGGGGGGACAAGAGAGGCCGAGTCCAGTTCAGGGGTTGGAGTTCTGCGGTTACCGGAGCGCtt  >  1:266540/1‑150 (MQ=255)

TCAATAAGCTTGTCGACCGGTACCAATATGAATAGCTCTTCAGGTTCGATAGGCTTGCTGAAGGTCTTCTTGTCGACCAGTACGTCATTCCAGTAGAGGAAAAGGTATTCCTCATCTATTTCCGGGTGCGAATTTGCCCACGGGTGGAACTCCACCCGCAGGGGGGAATAGGTTGCCCGGAACTCGACCGTGTTGGGTTTCCCCCCTTCAACATCGTCCACCACGCTTGGTACGGTTGGGGGGACAAGAGAGGCCGAGTCCAGTTCAGGGGTTGGAGTTCTGCGGTTACCGGAGCGCTT  >  NC_002947/2106514‑2106812

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`