Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 161,731 G→T 6.0% intergenic (‑226/+20) PP_0152 ← / ← PP_0153 hypothetical protein/hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_002947161,7310GT 6.0% 492.6 / 4.8 174intergenic (‑226/+20)PP_0152/PP_0153hypothetical protein/hypothetical protein
Reads supporting (aligned to +/- strand):  ref base G (95/61);  new base T (3/11);  total (98/76)
Fisher's exact test for biased strand distribution p-value = 8.61e-03
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 178 total aligned reads displayed.

ACTGCTGTGCCAGACAACTGTTAAGGCGAATTTTAATTTTTGTAAAACTGTACTGGTCGCAATCTGAAGCACGCGACTGCGCGTCTCTCGGGCAATAAAAAACCCGCTGCAGTCACCTGCAACGG‑G‑TTTGAGAAATTCACGGTGCCGGCGAAGCCGGCAAGCCGGGAGCTTAGCGCCAGAAAGGCTTGCTCAGCTCTTCGTAACGCTGCGACTCGCTGATACCGGCGTCTGCCAGCAGGCGGGAGTCCAGGCGAGCCAGTTGACGACGGCTGGTCATACGGCGCTGCCACAGCAGCAGG  >  NC_002947/161582‑161880
                                                                                                                                                       |                                                                                                                                                     
aCTGCTGTGCCAGACAACTGTTAAGGCGAATTTTAATTTTTGTAAAACTGTACTGGTCGCAATCTGAAGCACGCGACTGCGCGTCTCTCGGGCAATAAAAAACCCGCTGCAGTCACCTGCAACGG‑G‑TTTGAGAAATTCACGGTGCCGGCg                                                                                                                                                       >  2:3695143/1‑150 (MQ=255)
        gCCAGACAACTGTTAAGGCGAATTTTAATTTTTGTAAAACTGTACTGGTCGCAATCTGAAGCACGCGACTGCGCGTCTCTCGGGCAATAAAAAACCCGCTGCAGTCACCTGCAACGG‑G‑TTTGAGAAATTCACGGTGCCGGCGACGCCGGc                                                                                                                                               <  2:95356/150‑1 (MQ=255)
          cAGACAACTGTTAAGGCGAATTTTAATTTTTGTAAAACTGTACTGGTCGCAATCTGAAGCACGCGACTGCGCGTCTCTCGGGCAATAAAAAACCCGCTGCAGTCACCTGCAACGG‑G‑TTTGAGAAATTCACGGTGCCGGCGAAGCCGGCaa                                                                                                                                             >  1:2542215/1‑150 (MQ=255)
             aCAACTGTTAAGGCGAATTTTAATTTTTGTAATACTGTAGTGGTCGCAATCTGATGCACGCGACTGCGCGTCTGTCGGGCAATAAAATTCCCGCTGCTGTCAGCTGCATCGG‑G‑TTTGTGAAATTCACGGTGCCGGGGAAGCGGGCAAGcc                                                                                                                                          >  2:4025988/1‑150 (MQ=255)
              cAACTGTTATGGCTATTTTTATTTTTTTTAAAACTGTACGGGTCGAAATCTTAAGCACGCGACTGCGCGTCTCTCGGGAAATAAAAAACCCGCTGCAGTCACCTGCAACGG‑G‑TTTGAGAAATTCACGGTGCCGGCGAAGCCGGCAAGCCg                                                                                                                                         <  2:1886336/150‑1 (MQ=255)
                  tGTTACGGCTATTTTTATTTTTTTCAAAAGTGTCCGGGCCGAAATCTGACGCACGCGAGTGCGCTACGCTCGGGAAAAAAAAAACCCGCTGCAGTCACCTGCACGGG‑G‑TTTGAGAAATCCCGGGTGCGGGCTCCGCCGGCAAGCCGGGAg                                                                                                                                     <  2:313102/150‑1 (MQ=255)
                    ttAAGGCGAATTTTAATTTTTGTAAAACTGTACTGGTCGCAATCTGAAGCACGCGACTGCGCGTCTCTCGGGCAATAAAAAACCCGCTGCAGTCACCTGCAACGG‑G‑TTTGAGAAATTCACGGTGCCGGCGAAGCCGGCAAGCCGGGAGCt                                                                                                                                   >  2:5233331/1‑150 (MQ=255)
                         gCGAATTTTAATTTTTGTAAAACTGTACTGGTCGCAATCTGAAGCACGCGACTGCGCGTCTCTCGGGCAATAAAAAACCCGCTGCAGTCACCTGCAACGG‑G‑TTTGAGAAATTCACGGTGCCGGCGAAGCCGGCAAGCCGGGAGCTTAgcg                                                                                                                              >  1:499144/1‑150 (MQ=255)
                         gCGAATTTTAATTTTTGTAAAACTGTACTGGTCGCAATCTGAAGCACGCGACTGCGCGTCTCTCGGGCAATAAAAAACCCGCTGCAGTCACCTGCAACGG‑G‑TTTGAGAAATTCACGGTGCCGGCGAAGCCGGCAAGCCGGGAGCTTAgcg                                                                                                                              >  2:4839766/1‑150 (MQ=255)
                         gCGAATTTTAATTTTTGTAAAACTGTACTGGTCGCAATCTGAAGCACGCGACTGCGCGTCTCTCGGGCAATAAAAAACCCGCTGCAGTCACCTGCAACGG‑G‑TTTGAGAAATTCACGGTGCCGGCGAAGCCGGCAAGCCGGGAGCTTAgcg                                                                                                                              >  2:4152710/1‑150 (MQ=255)
                              ttttaattttTGTAAAACTGTACTGGTCGCAATCTGAAGCACGCGACTGCGCGTCTCTCGGGCAATAAAAAACCCGCTGCAGTCACCTGCAACGG‑G‑TTTGAGAAATTCACGGTGCCGGCGAAGCCGGCAAGCCGGGAGCTTAGCGCCAGa                                                                                                                         >  2:268475/1‑150 (MQ=255)
                               tttaattttTGTAAAACTGTACTGGTCGCAATCTGAAGCACGCGACTGCGCGTCTCTCGGGCAATAAAAAACCCGCTGCAGTCACCTGCAACGG‑G‑TTTGAGAAATTCACGGTGCCGGCGAAGCCGGCAAGCCGGGAGCTTAGCGCCAGaa                                                                                                                        >  1:873978/1‑150 (MQ=255)
                               tttaattttTGTAAAACTGTACTGGTCGCAATCTGAAGCACGCGACTGCGCGTCTCTCGGGCAATAAAAAACCCGCTGCAGTCACCTGCAACGG‑G‑TTTGAGAAATTCACGGTGCCGGCGAAGCCGGCAAGCCGGGAGCTTAGCGCCAGaa                                                                                                                        >  1:5060432/1‑150 (MQ=255)
                                ttatttttTGTAAAACTGTACTGGACGCAATCTGAAGCACGCGACTGCGCGTCTCTCGGGCAATAAAAAACCCGCGGCAGTCACCTGCAACGT‑G‑TTTGAGAAATTCCCGGTGCCGGCGAAGCCGGCAAGCCGGGAGCTTAGCGCCAGaaa                                                                                                                       <  2:1632464/150‑1 (MQ=255)
                                   attttTGTAAAACTGTACTGGTCGCAATCTGAAGCACGCGACTGCGCGTCTCTCGGGCAATAAAAAACCCGCTGCAGTCACCTGCAACGG‑G‑TTTGAGAAATTCACGGTGCCGGCGAAGCCGGCAAGCCGGGAGCTTAGCGCCAGAAAGGc                                                                                                                    >  2:3953273/1‑150 (MQ=255)
                                    tttttGTAAAACTGTACTGGTCGCAATCTGAAGCACGCGACTGCGCGTCTCTCGGGCAATAAAAAACCCGCTGCAGTCACCTGCAACGG‑G‑TTTGAGAAATTCACGGTGCCGGCGAAGCCGGCAAGCCGGGAGCTTAGCGCCAGAAAGGCt                                                                                                                   >  1:4068840/1‑150 (MQ=255)
                                    tttttGTAAAACTGTACTGGTCGCAATCTGAAGCACGCGACTGCGCGTCTCTCGGGCAATAAAAAACCCGCTGCAGTCACCTGCAACGG‑G‑TTTGAGAAATTCACGGTGCCGGCGAAGCCGGCAAGCCGGGAGCTTAGCGCCAGAAAGGCt                                                                                                                   >  2:2964995/1‑150 (MQ=255)
                                      tttGTAAAACTGTAGTGGGCGCAATCTTACGCCCGCGAGTGCGCGTCTCTCGGGAAATAAAAAACCCGCTGCATCCACCTGCAACGG‑T‑TTTCACAAATACACGGTGCCGGCGACGCCGGCAAGCCGGGAGCTTAGCGCCAGAAAGGCTTg                                                                                                                 <  2:129972/150‑1 (MQ=255)
                                       ttGTAAAACTGTACTGGTCGCAATCTGAAGCACGCGACTGCGCGTCTCTCGGGCAATAAAAAACCCGCTGCAGTCACCTGCAACGG‑G‑TTTGAGAAATTCACGGTGCCGGCGAAGCCGGCAAGCCGGGAGCTTAGCGCCAGAAAGGCTTGc                                                                                                                >  1:53536/1‑150 (MQ=255)
                                       ttGTAAAACTGTACTGGTCGCAATCAGAAGCACGCGACTGCGCGTCTCTCGGGCAATAAAAAACCCGCTGCAGTCACCTGCAACGG‑G‑TTTGAGAAATTCACGGTGCCGGCGAAGCCGGCAAGCCGGGAGCTTAGCGCCAGAAAGGCTTGc                                                                                                                >  1:53462/1‑150 (MQ=255)
                                        tGTAAACCTGTACGGGTCGCAAGCTGACGCCCGCGACTGCGCGTCTCAGGGGCAATAAAAACCCCGCGGCCGGCCCCGGCAAGGG‑G‑TTTGAGAAAGCCCCGGGGCCGGCTACGCGGGCAAGCCGGGAGCTTAGCGCCAGAAAGGCTTGCt                                                                                                               <  2:3404171/150‑1 (MQ=255)
                                        tGTAAAATTGTACTGTTCCAAATCTGACGCAGGCGACTGCGCTTCTCTCGGGAAATAAAAAACCGGCTGCAGTCACCTGCAACGG‑T‑TTTGATAAATTCCGGGTGCCGGCGACGCGGGCAAGCCGGGAGCTTAGCGCCAGAAAGGCTTGCt                                                                                                               <  1:3596386/150‑1 (MQ=255)
                                          tAAAACTTTACGGTTCGCATTCTTATGCACGCGACTGGGGTTCTCTGGGGCAATAAAAACCCCGCTGCAGTCACCTGCAACGG‑T‑TTTGAGAATTGCCCGGGGCCGGCGACGCGGGCAAGCCGGGAGCTTAGCGCCAGAAAGGCTTGCTCa                                                                                                             <  2:1572380/150‑1 (MQ=255)
                                           aaaaCTGTACGGGTCGCAATCTGAAGCACGCGACTGCGCGTCTCTCGGGCAATAAAAAACCCGCTGCAGTCACCTGCAACGG‑G‑TTTGATAAATTCACGGTGCGGGCGAAGCCGGCAAGCCGGGAGCTTAGCGCCAGAAAGGCTTGCTCAg                                                                                                            <  2:3138319/150‑1 (MQ=255)
                                             atcTGTACGGGTCTAAATCTTAAGCCCGCTAGTGCGGGTCTCTCGGTAAATAAAAAACCCGCTGACGACACCTGAAAGGG‑T‑TTTGATAAATTCACGGTGCGGGCGAAGCCGGCAAGCCGGGAGCTTAGCGCCAGAAAGGCTTGCTCAGct                                                                                                          <  2:2001371/148‑1 (MQ=255)
                                             aaCTTTAGTGGTCGCAATCTGAAGCACGCGACTGCGCGTCTCTGGGGCAATAAAAAACCCGCTGCAGTCACCTGCACCGG‑G‑TTTGAGAAATTCACGGTGCCGGCGAAGCCGGCAAGCCGGGAGCTTAGCGCCAGAAAGGCTTGCTCAGct                                                                                                          <  2:3821240/150‑1 (MQ=255)
                                                 gTAGTGGTCGAATTCTTACGCCCGCGACTGCGCGTCTCTGGGCCAAAAAAAAACCCGCGGCAGTCACCTGCACCGG‑T‑TTTGAGAAATCCCGGGGGCCGGCTACGCCGGCAAGCCGGGAGCTTAGCGCCAGAAAGGCTTGCTCAGCTCTTc                                                                                                      <  1:4416372/150‑1 (MQ=255)
                                                 gTACTGGCCCCAATCGCACGCCCGCGACGGCGCGTCCCGCGGCCAAAAAAAAACCCGCTGCAGCCACCTGCAAGGG‑G‑TTTGAGAAATTCACGGTGCCGGCGAAGCCGCCAAGCCGGGAGCTTAGCGCCAGAAAGGCTTGCTCAGCTCTTc                                                                                                      <  1:4417114/150‑1 (MQ=255)
                                                     tGGTCGCAATCTGAAGCACGCGACTGCGCGTCTCTCGGGCAATAAAAAACCCGCTGCAGTCACCTGCAACGG‑G‑TTTGAGAAATTCACGGTGCCGGCGAAGCCGGCAAGCCGGGAGCTTAGCGCCAGAAAGGCTTGCTCAGCTCTTCGTaa                                                                                                  >  1:847319/1‑150 (MQ=255)
                                                     tGGTCGCAATCTGAAGCACGCGACTGCGCGTCTCTCGGGCAATAAAAAACCCGCTGCAGTCACCTGCAACGG‑G‑TTTGAGAAATTCACGGTGCCGGCGAAGCCGGCAAGCCGGGAGCTTAGCGCCAGAAAGGCTTGCTCAGCTCTTCGTaa                                                                                                  >  1:1042774/1‑150 (MQ=255)
                                                     tGGTCGCAATCTGAAGCACGCGACTGCGCGTCTCTCGGGCAATAAAAAACCCGCTGCAGTCACCTGCAACGG‑G‑TTTGAGAAATTCACGGTGCCGGCGAAGCCGGCAAGCCGGGAGCTTAGCGCCAGAAAGGCTTGCTCAGCTCTTCGTaa                                                                                                  >  1:4363444/1‑150 (MQ=255)
                                                       gTCGCAATCTGAAGCACGCGACTGCGCGTCTCTCGGGCAATAAAAAACCCGCTGCAGTCACCTGCAACGG‑G‑TTTGAGAAATTCACGGTGCCGGCGAAGCCGGCAAGCCGGGAGCTTAGCGCCAGAAAGGCTTGCTCAGCTCTTCGTAACg                                                                                                >  1:2099072/1‑150 (MQ=255)
                                                          gCAATCTGAAGCACGCGACTGCGCGTCTCTCGGGCAATAAAAAACCCGCTGCAGTCACCTGCAACGG‑G‑TTTGAGAAATTCACGGTGCCGGCGAAGCCGGCAAGCCGGGAGCTTAGCGCCAGAAAGGCTTGCTCAGCTCTTCGTAACGCTg                                                                                             >  2:3196736/1‑150 (MQ=255)
                                                           cAATCTGAAGCCCGCGACGGCGCGCCTCTGGGGAAATAAAAAACCCGCGGCCGTCACCTGCAACGG‑G‑TTTGAGAAATGCACGGTGCCGGCGACGCCGGCAAGCCGGGAGCTTAGCGCCAGAAAGGCTTGCTCAGCTCTTCGTAACGCTGc                                                                                            <  2:309528/150‑1 (MQ=255)
                                                           cAATCTGAAGCACGCGACTGCGCGTCTCTCGGGAAATAAAAAACCCGCTGCCGTCACCTGCAACGG‑G‑TTGGAGAAATTCACGGTGCCGGCGACGCCGGCAAGCCGGGAGCTTAGCGCCAGAAAGGCTTGCTCAGCTCTTCGTAACGCTGc                                                                                            <  2:5261396/150‑1 (MQ=255)
                                                              tCTGAAGCACGCGACTGCGCGTCTCTCGGGCAATAAAAAACCCGCTGCAGTCACCTGCAACGG‑G‑TTTGAGAAATTCACGGTGCCGGCGAAGCCg                                                                                                                                                 >  2:5023923/1‑94 (MQ=255)
                                                              tCTGAAGCACGCGACTGCGCGTCTCTCGGGCAATAAAAAACCCGCTGCAGTCACCTGCAACGG‑G‑TTTGAGAAATTCACGGTGCCGGCGAAGCCGGCAAGCCGGGAGCTTAGCGCCAGAAAGGCTTGCTCAGCTCTTCGTAACGCTGCGAc                                                                                         >  2:5098909/1‑150 (MQ=255)
                                                                tGAAGCACGCGACTGCGCGTCTCTCGGGCAATAAAAAACCCGCTGCAGTCACCTGCAACGG‑G‑TTTGAGAAATTCACGGTGCCGGCGAAGCCGGCAAGCCGGGAGCTTAGCGCCAGAAAGGCTTGCTCAGCTCTTCGTAACGCTGCGACTc                                                                                       >  2:2816411/1‑150 (MQ=255)
                                                                  aaGCCCGCGACTGCGCGTCTCTCGGGAAATAAAAAACCCGCTGCAGTCACCTGCACGGG‑T‑TTTGATAAATTCAGGGTGCCGGCGAAGCCGCCAAGCCGGGAGCTTAGCGCCAGAAAGGCTTGCTCAGCTCTTCGTAACGCTGCGACTCGc                                                                                     <  1:3146466/150‑1 (MQ=255)
                                                                  aaGCACGCGACTGCGCGTCTCTCGGGCAATAAAAAACCCGCTGCAGTCACCTGCAACGG‑G‑TTTGAGAAATTCACGGTGCCGGCGAAGCCGGCAAGCCGGGAGCTTAGCGCCAGAAAGGCTTGCTCAGCTCTTCGTAACGCTGCGACTCGc                                                                                     >  1:5112726/1‑150 (MQ=255)
                                                                     cACGCGACTGCGCGTCTCTCGGGCAATAATAATCCCGCTGCAGTCACCTGCAACGG‑G‑TTTGAGAAATTCACGGTGCCGGCGAAGCCGGCAAGCCGGGAGCTTAGCGCCAGAAAGGCTTGCTCAGCTCTTCGTAACTCTGAGACTCGCTGa                                                                                  >  1:1932539/1‑150 (MQ=255)
                                                                     cACGCGACTGCGCGTCTCTCGGGCAATAAAAAACCCGCTGCAGTCACCTGCAACGG‑G‑TTTGAGAAATTCACGGTGCCGGCGAAGCCGGCAAGCCGGGAGCTTAGCGCCAGAAAGGCTTGCTCAGCTCTTCGTAACGCTGCGACTCGCTGa                                                                                  <  2:4926449/150‑1 (MQ=255)
                                                                      aCGCGACTGCGCGTCTCTCGGGCAATAAAAAACCCGCTGCAGTCACCTGCAACGG‑G‑TTTGAGAAATTCACGGTGCCGGCGAAGCCGGCAAGCCGGGAGCTTAGCGCCAGAAAGGCTTGCTCAGCTCTTCGTAACGCTGCGACTCGCTGAt                                                                                 >  1:3059994/1‑150 (MQ=255)
                                                                      aCGCGACTGCGCGTCTCTCGGGCAATAAAAAACCCGCTGCAGTCACCTGCAACGG‑G‑TTTGAGAAATTCACGGTGCCGGCGAAGCCGGCAAGCCGGGAGCTTAGCGCCAGAAAGGCTTGCTCAGCTCTTCGTAACGCTGCGACTCGCTGAt                                                                                 >  1:2936694/1‑150 (MQ=255)
                                                                      aCGCGACTGCGCGTCTCTCGGGCAATAAAAAACCCGCTGCAGTCACCTGCAACGG‑G‑TTTGAGAAATTCACGGTGCCGGCGAAGCCGGCAAGCCGGGAGCTTAGCGCCAGAAAGGCTTGCTCAGCTCTTCGTAACGCTGCGACTCGCTGAt                                                                                 >  1:205962/1‑150 (MQ=255)
                                                                          gacggggcgTATCTCGGCAAAAAAAAACCCCGCGGCCTCCACCT‑CAATGG‑GTTTTGACAAATTCCCGGGGCGGGCGACGCCGCCAAGCCGGGAGCTTAGCGCCAGAAAGGCTTGCTCAGCTCTTCGTAACGCTGCGACTCGCTGATACCg                                                                             <  1:1100603/144‑1 (MQ=255)
                                                                                    ctctcGGGCAATAAAAAACCCGCTGCAGTCACCTGCACGGG‑T‑TTTGAGAAATTCACGGTGCCGGCGAAGCCGGCAAGCCGGGAGCTTAGCGCCAGAAAGGCTTGCTCAGCTCTTCGTAACGCTGCGACTCGCTGATACCGGCGTCTGCca                                                                   <  1:2229744/150‑1 (MQ=255)
                                                                                    ctctcGGGCAATAAAAAACCCGCTGCAGTCACCTGCACCGG‑G‑TTTGAGAAATTCAGGGTGCCGGCGACGCCGGCAAGCCGGGAGCTTAGCGCCAGAAAGGCTTGCTCAGCTCTTCGTAACGCTGTGACTCGCTGATACCGGCGTCTGCca                                                                   <  1:5420482/150‑1 (MQ=255)
                                                                                     tctcGGGCAATAAAAAACCCGCTGCAGTCACCTGCAACGG‑G‑TTTGAGAAATTCACGGTGCCGGCGAAGCCGGCAAGCCGGGAGCTTAGCGCCAGAAAGGCTTGCTCAGCTCTTCGTAACGCTGCGACTCGCTGATACCGGCGTCTGCcag                                                                  >  1:2306004/1‑150 (MQ=255)
                                                                                     actcGGGCAATAAAAACCCCGCTGCAGTCACCTGCAACGG‑G‑TTTGAGAAATCCACGGTGCCGGCGAAGCCGGCAAGCCGGGAGCTTAGCGCCAGAAAGGCTTGCTCAGCTCTTCGTAACGCTGCGACTCGCTGATACCGGCGTCTGCcag                                                                  <  2:2656865/149‑1 (MQ=255)
                                                                                       tcGGGCAATAAAAAACCCGCTGCAGTCACCTGCAACGG‑G‑TTTGAGAAATTCACGGTGCCGGCGAAGCCGGCAAGCCGGGAGCTTAGCGCCAGAAAGGCTTGCTCAGCTCTTCGTAACGCTGCGACTCGCTGATACCGGCGTCTGCcagca                                                                >  1:754329/1‑150 (MQ=255)
                                                                                       tcGGGCAATAAAAAACCCGCTGCAGTCACCTGCAACGG‑G‑TTTGAGAAATTCACGGTGCCGGCGAAGCCGGCAAGCCGGGAGCTTAGCGCCAGAAAGGCTTGCTCAGCTCTTCGTAACGCTGCGACTCGCTGATACCGGCGTCTGCcagca                                                                >  1:754487/1‑150 (MQ=255)
                                                                                         gggCAATAAAAAACCCGCAGCAGTCACCTGCAACGG‑G‑TTTGAGAAATTCACGGTGCCGGCGAAGCCGGCAAGCCGGGAGCTTAGCGCCAGAAAGGCTTGCTCAGCTCTTCGTAACGCTGCGACTCGCTGATACCGGCGTCTGCCAGCAgg                                                              >  2:2779412/1‑150 (MQ=255)
                                                                                           gaaaTAAAAAACCCGCTGCAGTCACCTGAAAGGG‑G‑TTTGAGAATTTCAGGGTGCGGGCGACGCGGGCAAGCCGGGAGCTTAGCGCCAGAAAGGCTTGCTCAGCTCTTCGTAACGCTGCGACTCGCTGATACCGGCGTCTGCCAGCAGGCg                                                            <  2:1038891/148‑1 (MQ=255)
                                                                                           gCAATAAAAAACCCGCTGCAGTCACCTGCAACGG‑G‑TTTGAGAAATTCACGGTGCCGGCGAAGCCGGCAAGCCGGGAGCTTAGCGCCAGAAAGGCTTGCTCAGCTCTTCGTAACGCTGCGACTCGCTGATACCGGCGTCTGCCAGCAGGCg                                                            >  1:2762022/1‑150 (MQ=255)
                                                                                           gCAATAAAAAACCCGCTGCAGTCACCTGCAACGG‑G‑TTTGAGAAATTCACGGTGCCGGCGAAGCCGGCAAGCCGGGAGCTTAGCGCCAGAAAGGCTTGCTCAGCTCTTCGTAACGCTGCGACTCGCTGATACCGGCGTCTGCCAGCAGGCg                                                            >  2:2452755/1‑150 (MQ=255)
                                                                                            cAATAAAAACCCCGCTGCAGTCACCTGCAACGG‑G‑TTTGAGAAAGCCACGGTGCCGGCGAAGCCGGCAAGCCGGGAGCTTAGCGCCAGAAAGGCTTGCTCAGCTCTTCGTAACGCTGCGACTCGCTGATACCGGCGTCTGCCAGCAGGCgg                                                           <  2:5290762/150‑1 (MQ=255)
                                                                                            cAATAAAAAACCCGCTGCAGTCACCTGCAAGGG‑T‑TTTGATAAATTCAGGGTGCGGGCGAAGCCGGCAAGCCGGGAGCTTAGCGCCAGAAAGGCTTGCTCAGCTCTTCGTAACGCTGCGACTCGCTGATACCGGCGTCTGCCAGCAGGCgg                                                           <  1:3080526/150‑1 (MQ=255)
                                                                                            cAAAAAAAAACCCGCTGCAGTCACCTGCAACGG‑G‑TTTGAGAAATTCCCGGTGCCGGCGAAGCCGGCAAGCCGGGAGCTTAGCGCCAGAAAGGCTTGCTCAGCTCTTCGTAACGCTGCGACTCGCTGATACCGGCGTCTGCCAGCAGGCgg                                                           <  2:1022596/150‑1 (MQ=255)
                                                                                             aaTAAAAAACCCGCTGCAGTCACCTGCAACGG‑G‑TTTGAGAAATTCACGGTGCCGGCGAAGCCGGCAAGCCGGGAGCTTAGCGCCAGAAAGGCTTGCTCAGCTCTTCGTAACGCTGCGACTCGCTGATACCGGCGTCTGCCAGCAGGCggg                                                          >  2:1090771/1‑150 (MQ=255)
                                                                                             aaTAAAAAACCCGCTGCAGTCACCTGCAACGG‑G‑TTTGAGAAATTCACGGTGCCGGCGAAGCCGGCAAGCCGGGAGCTTAGCGCCAGAAAGGCGTGCTCAGCTCTTCGTAACGCTGCGACTCGCTGATACCGGCGTCTGCCAGCAGGCggg                                                          >  2:1626849/1‑150 (MQ=255)
                                                                                              aTAAAAACCCCGCGGCAGCCACCGGCAAGGG‑G‑TTTGAGAAATCCCCGGGGCCGGCGACGCCGGCAAGCCGGGAGCTTAGCGCCAGAAAGGCTTGCTCAGCTCTTCGTAACGCTGCGACTCGCTGATACCGGCGTCTGCCAGCAGGCGGGa                                                         <  2:705993/150‑1 (MQ=255)
                                                                                              aTAAAAAACCCGCTGCAGTCACCTTCAACGG‑G‑TTTTATAAATTCACGGTGCCGGCTAAGCCGGCAAGCCGGGAGCTTAGCGCCAGAAAGGCTTGCTCAGCTCTTCGTAACGCTGCGACTCGCTGATACCGGCGTCTGCCAGCAGGCGGGa                                                         <  2:3853664/150‑1 (MQ=255)
                                                                                              aTAAAAAACCCGCTGCAGTCACCTGCAACGG‑G‑TTTGAGAATTTCCCGGTGCCGGCGAAGCCGGCAAGCCGGGAGCTTAGCGCCAGAAAGGCTTGCTCAGCTCTTCGTAACGCTGCGACTCGCTGATACCGGCGTCTGCCAGCAGGCGGGa                                                         <  1:4490922/150‑1 (MQ=255)
                                                                                              aTAAAAAACCCGCGGCTCTCA‑CTTCAACGGTT‑TTTGATAATTTCGGGGTGCCGGCCACGCCGGCAAGCCGGGAGCTTAGCGCCAGAAAGGCTTGCTCAGCTCTTCGTAACGCTGCGACTCGCTGATACCGGCGTCTGCCAGCAGGCGGGa                                                         <  1:4219615/150‑1 (MQ=255)
                                                                                               ttaaaaaCCCGCTGCAGTCACCTGCAACGG‑G‑TTTGAGAAATTCACGGTGCCGGCGAAGCCGGCAAGCCGGGAGCTTAGCGCCAGAAAGGCTTGCTCAGCTCTTCGTAACGCTGCGACTCGCTGATACCGGCGTCTGCCAGCAGGCGGGAg                                                        >  2:1215880/3‑150 (MQ=255)
                                                                                               tAAAAAACCCGCTGCAGTCACCTGCAACGG‑G‑TTTGAGAAATTCACGGTGCCGGCGAAGCCGGCAAGCCGGGAGCTTAGCGCCAGAAAGGCTTGCTCAGCTCTTCGTAACGCTGCGACTCGCTGATACCGGCGTCTGCCAGCAGGCGGGAg                                                        >  1:908744/1‑150 (MQ=255)
                                                                                               tAAAAAACCCGCTGCAGTCACCTGCAACGG‑G‑TTTGAGAAATTCACGGTGCCGGCGAAGCCGGCAAGCCGGGAGCTTAGCGCCAGAAAGGCTTGCTCAGCTCTTCGTAACGCTGCGACTCGCTGATACCGGCGTCTGCCAGCAGGCGGGAg                                                        >  2:1241264/1‑150 (MQ=255)
                                                                                                 aaaaaCCCGCTGCAGTCACCTGCAACGG‑G‑TTTGAGAAATTCACGGTGCCGGCGAAGCCGGCAAGCCGGGAGCTTAGCGCCAGAAAGGCTTGCTCAGCTCTTCGTAACGCTGCGACTCGCTGATACCGGCGTCTGCCAGCAGGCGGGAgag                                                      >  1:3853664/1‑148 (MQ=255)
                                                                                                 aaaaaCCCGCTGCAGTCACCTGCAACGG‑G‑TTTGAGAAATTAACTGTGCCGGCGAAGCCGGCAAGCCGGGAGCTTAGCGCCAGAAAGGCTTGCTAAGCTCTTCGTAACGCTGCGACTCGCTGATACCGGCGTCTCCCAGCAGGCGGGAGTc                                                      >  1:3761738/1‑150 (MQ=255)
                                                                                                  aaaaCCCGCTGCAGTCACCTGAAACGT‑T‑TTTCACAAATTCACGGTCCCGGCGAAGCCGGCAAGCCGGGAGCTTAGCGCCAGAAAGGCTTGCTCAGCTCTTCGAAACGCTGCGACTCGCTGATACCGGCGTCTGCCAGCAGGCGGGAGAcc                                                     <  2:873978/150‑1 (MQ=255)
                                                                                                   aaaCCCGCTGCAGTCACCTGCAACGG‑G‑TTTGAGAAATTCACGGTGCCGGCGAAGCCGGCAAGCCGGGAGCTTAGCGCCAGAAAGGCTTGCTCAGCTCTTCGTAACGCTGCGACTCGCTGATACCGGCGTCTGCCAGCAGGCGGGAGTCCa                                                    >  1:1549827/1‑150 (MQ=255)
                                                                                                     ccccGCGCCAGACACCTGCAAGGT‑T‑TTTTATAAATCCCCGGTGCCGGCTACGCCGCCAAGCCGGGAGCTTAGCGCCAGAAAGGCTTGCTCAGCTCTTCGTAACGCTGCGACTCGCTGATACCGGCGTCTGCCAGCAGGCGGGAGTCCAgg                                                  <  1:4617690/149‑1 (MQ=255)
                                                                                                     aCCCGCTGCAGTCACCTGCAAGGG‑T‑TTTGAGAAATTCAGGGTGCGGGCTACGCCGGCAAGCCGGGAGCTTAGCGCCAGAAAGGCTTGCTCAGCTCTTCGTAACGCTGCGACTCGCTGATACCGGCGTCTGCCAGCAGGCGGGAGTCCAgg                                                  <  1:2194948/150‑1 (MQ=255)
                                                                                                        ctcTGCAGTCACCTGAATCGG‑G‑TTTGAGAATTTCACGGTGCGGGCGAAGCCGGCAAGCCGGGAGCTTAGCGCCAGAAAGGCTTGCTCAGCTCTTCGTAACGCTGCGACTCGCTGATACCGGCGTCTGCCAGCAGGCGGGAGTCCAGGCGa                                               <  2:2936694/148‑1 (MQ=255)
                                                                                                        cGCTGCTGTCACCTGCAACGG‑G‑TTTGAGAAATTCCCGGTGCCGGCGAAGCCGGCAAGCCGGGAGCTTAGCGCCAGAAAGGCTTGCTCAGCTCTTCGTAACGCTGCGACTCGCTGATACCGGCGTCTGCCAGCAGGCGGGAGTCCAGGCGa                                               <  2:3567028/150‑1 (MQ=255)
                                                                                                        cGCTGCAGTCACCTGCAACGG‑G‑TTGGAGAAATTCACGGTGCCGGCGAAGCCGGCAAGCCGGGAGCTTAGCGCCAGAAAGGCTTGCTCAGCTCTTCGTAACGCTGCGACTCGCTGATACCGGCGTCTGCCAGCAGGCGGGAGTCCAGGCGa                                               <  2:1755992/150‑1 (MQ=255)
                                                                                                              aGTCACCTGCAACGG‑G‑TTTGAGAAATTCACGGTGCCGGCGAAGCCGGCAAGCCGGGAGCTTAGCGCCAGAAAGGCTTGCTCAGCTCTTCGTAACGCTGCGACTCGCTGATACCGGCGTCTGCCAGCAGGCGGGAGTCCAGGCGAGCCAGt                                         >  1:1400014/1‑150 (MQ=255)
                                                                                                                gtgacTGCAGCGG‑G‑TTTGAGAAATTCACGGTGCCGGCGAAGCCGGCAAGCCGGGAGCTTAGCGCCAGAAAGGCTTGCTCAGCTCTTCGTAACGCTGCGACTCGCTGATACCGGCGTCTGCCAGCAGGCGGGAGTCCAGGCGAGCCAGTTg                                       >  1:5393584/5‑150 (MQ=255)
                                                                                                                    cTGCAACGG‑G‑TTTGAGAAATTCACGGTGCCGGCGAAGCCGGCAAGCCGGGAGCTTAGCGCCAGAAAGGCTTGCTCAGCTCTTCGTAACGCTGCGACTCGCTGATACCGGCGTCTGCCAGCAGGCGGGAGTCCAGGCGAGCCAGTTgacga                                   >  1:619971/1‑150 (MQ=255)
                                                                                                                    cTGCAACGG‑G‑TTTGAGAAATTCACGGTGCCGGCGAAGCCGGCAAGCCGGGAGCTTAGCGCCAGAAAGGCTTGCTCAGCTCTTCGTAACGCTGCGACTCGCTGATACCGGCGTCTGCCAGCAGGCGGGAGTCCAGGCGAGCCAGTTgacga                                   >  2:1620941/1‑150 (MQ=255)
                                                                                                                          cGG‑G‑TTTGAGAAATTCACGGTGCCGGCGAAGCCGGCAAGCCGGGAGCTTAGCGCCAGAAAGGCTTGCTCAGCTCTTCGTAACGCTGCGACTCGCTGATACCGGCGTCTGCCAGCAGGCGGGAGTCCAGGCGAGCCAGTTGACGACGGCTg                             >  1:3669197/1‑150 (MQ=255)
                                                                                                                          cGG‑G‑TTTGAGAAATCCCCGGTGCCGGCTACGCCGGCAAGCCGGGAGCTTAGCGCCAGAAAGGCTTGCTCAGCTCTTCGTAACGCTGCGACTCGCTGATACCGGCGTCTGCCAGCAGGCGGGAGTCCAGGCGAGCCAGTTGACGACGGCTg                             <  2:1372709/150‑1 (MQ=255)
                                                                                                                           gg‑g‑TTTGATAAAGTCCCGGTGCCGGCGAAGCCGGCAAGCCGGGAGCTTAGCGCCAGAAAGGCTTGCTCAGCTCTTCGTAACGCTGCGACTCGCTGATACCGGCGTCTGCCAGCAGGCGGGAGTCCAGGCGAGCCAGTTGACGACGGCTgg                            <  2:1504557/150‑1 (MQ=255)
                                                                                                                           gg‑g‑TTTGAGAAATTCACGGTGCCGGCGAAGCCGGCAAGCCGGGAGCTTAGCGCCAGAAAGGCTTGCTCAGCTCTTCGTAACGCTGCGACTCGCTGATACCGGCGTCTGCcagcag                                                               >  1:4962432/1‑115 (MQ=255)
                                                                                                                              g‑TTTGAGAAATTCACGGTGCCGGCGAAGCCGGCAAGCCGGGAGCTTAGCGCCAGAAAGGCTTGCTCAGCTCTTCGTAACGCTGCGACTCGCTGATACCGGCGTCTGCCAGCAGGCGGGAGTCCAGGCGAGCCAGTTGACGACGGCTGGTc                          >  1:3087165/1‑150 (MQ=255)
                                                                                                                                  tGAGAATTTCAGGGTGCCGGCGACGCCGGCAAGCCGGGAGCTTAGCGCCAGAAAGGCTTGCTCAGCTCTTCGTAACGCTGCGACTCGCTGATACAGGCGTCTGCCAGCAGGCGGGAGTCCAGGCGAGCCAGTTGACGACGGCTGGTCATa                       <  2:499144/150‑1 (MQ=255)
                                                                                                                                    agaAATTCACGGTGCCGGCGAAGCCGGCAAGCCGGGAGCTTAGCGCCAGAAAGGCTTGCTCAGCTCTTCGTAACGCTGCGACTCGCTGATACCGGCGTCTGCCAGCAGGCGGGAGTCCAGGCGAGCCAGTTGACGACGGCTGGTCATACg                     >  1:2580784/1‑150 (MQ=255)
                                                                                                                                      aaaTTCACGGTGCCGGCGAAGCCGGCAAGCCGGGAGCTTAGCGCCAGAAAGGCTTGCTCAGCTCTTCGTAACGCTGCGACTCGCTGATACCGGCGTCTGCCAGCAGGCGGGAGTCCAGGCGAGCCAGTTGACGACGGCTGGTCATACGgc                   >  1:1658183/1‑150 (MQ=255)
                                                                                                                                        aTTCACGGTGCCGGCGAAGCCGGCAAGCCGGGAGCTTAGCGCCAGAAAGGCTTGCTCAGCTCTTCGTAACGCTGCGACTCGCTGATACCGGCGTCTGCCAGCAGGCGGGAGTCCAGGCGAGCCAGTTGACGACGGCTGGTCATACGgcgc                 >  2:3302363/1‑150 (MQ=255)
                                                                                                                                         ttccgggTGCCGGCGACGCGGGCAAGCCGGGAGCTTAGCGCCAGAAAGGCTTGCTCAGCTCTTCGTAACGCTGCGACTCGCTGATACCGGCGTCTGCCAGCAGGCGGGAGTCCAGGCGAGCCAGTTGACGACGGCTGGTCATACGGCGCt                <  1:4152710/145‑1 (MQ=255)
                                                                                                                                           cACGGTGCCGGCGAAGCCGGCAAGCCGGGAGCTTAGCGCCAGAAAGGCTTGCTCAGCTCTTCGTAACGCTGCGACTCGCTGATACCGGCGTCTGCCAGCAGGCGGGAGTCCAGGCGAGCCAGTTGACGACGGCTGGTCATACGGCGCTGc              <  2:5060448/150‑1 (MQ=255)
                                                                                                                                           cACGGTGCCGGCGAAGCCGGCAAGCCGGGAGCTTAGCGCCAGAAAGGCTTGCTCAGCTCTTCGTAACGCTGCGACTCGCTGATACCGGCGTCTGCCAGCAGGCGGGAGTCCAGGCGAGCCAGTTGACGACGGCTGGTCATACGGCGCTGc              >  1:242356/1‑150 (MQ=255)
                                                                                                                                               ttGCCGGCTTCGCCGGCAAGCCGGGAGCTTAGCGCCAGAAAGGCTTGCTCAGCTCTTCGTAACGCTGCGACTCGCTGATACCGGCGTCTGCCAGCAGGCGGGAGTCCAGGCGAGCCAGTTGACGACGGCTGGTCATACGGCGCTGCcacc          >  2:18390/2‑149 (MQ=255)
                                                                                                                                                ggcgggCGACGCCGGCAAGCCGGGAGCTTAGCGCCAGAAAGGCTTGCTCAGCTCTTCGTAACGCTGCGACTCGCTGATACCGGCGTCTGCCAGCAGGCGGGAGTCCAGGCGAGCCAGTTGACGACGGCTGGTCATACGGCGCTGCCAcag         <  1:4839766/146‑1 (MQ=255)
                                                                                                                                                  ccGGCGAAGCCGGCAAGCCGGGAGCTTAGCGCCAGAAAGGCTTGCTCAGCTCTTCGTAACGCTGCGACTCGCTGATACCGGCGTCTGCCAGCAGGCGGGAGTCCAGGCGAGCCAGTTGACGACGGCTGGTCATACGGCGCTGCCAcagca       >  2:4546841/1‑150 (MQ=255)
                                                                                                                                                   cGGCGAAGCCGGCAAGCCGGGAGCTTAGCGCCAGAAAGGCTTGCTCAGCTCTTCGTAACGCTGCGACTCGCTGATACCGGCGTCTGCCAGCAGGCGGGAGTCCAGGCGAGCCAGTTGACGACGGCTGGTCATACGGCGCt                <  1:1228491/140‑1 (MQ=255)
                                                                                                                                                     gCGAAGCCGGCAAGCCGGGAGCTTAGCGCCAGAAAGGCTTGCTCAGCTCTTCGTAACGCTGCGACTCGCTGATACCGGCGTCTGCCAGCAGGCGGGAGTCCAGGCGAGCCAGTTGACGACGGCTGGTCATACGGCGCTGCCAcagcagca    >  1:4305387/1‑150 (MQ=255)
                                                                                                                                                       gAAGCCGGCAAGCCGGGAGCTTAGCGCCAGAAAGGCTTGCTCAGCTCTTCGTAACGCTGCGACTCGCTGATACCGGCGTCTGCCAGCAGGCGGGAGTCCAGGCGAGCCAGTTGACGACGGCTGGTCATACGGCGCTGCCAcagcag      <  2:4610199/146‑1 (MQ=255)
                                                                                                                                                       gAAGCCGGCAAGCCGGGAGCTTAGCGCCAGAAAGGCTTGCTCAGCTCTTCGTAACGCTGCGACTCGCTGATACCGGCGTCTGCCAGCAGGCGGGAGTCCAGGCGAGCCAGTTGACGACGGCTGGTCATACGGCGCTGCCACAGCAGCAgg  >  1:3265375/1‑150 (MQ=255)
                                                                                                                                                       |                                                                                                                                                     
ACTGCTGTGCCAGACAACTGTTAAGGCGAATTTTAATTTTTGTAAAACTGTACTGGTCGCAATCTGAAGCACGCGACTGCGCGTCTCTCGGGCAATAAAAAACCCGCTGCAGTCACCTGCAACGG‑G‑TTTGAGAAATTCACGGTGCCGGCGAAGCCGGCAAGCCGGGAGCTTAGCGCCAGAAAGGCTTGCTCAGCTCTTCGTAACGCTGCGACTCGCTGATACCGGCGTCTGCCAGCAGGCGGGAGTCCAGGCGAGCCAGTTGACGACGGCTGGTCATACGGCGCTGCCACAGCAGCAGG  >  NC_002947/161582‑161880

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑